Journal of Cellular Physiology最新文献

Exercise and diet are the best-known methods for attenuating aging-related health decline. However, exercise in older age has diminished gains of strength and agility, and a danger of unrepaired muscle damage. Improving the understanding of age-related differences in response to exercise, our results demonstrate that in old mice, downhill treadmill (eccentric) exercise causes increased influx of CD45+ cells (inflammation) and fibrotic index (fibrosis) in the heart and skeletal muscles. To explain these changes, we identified newly synthesized proteins through bio-orthogonal noncanonical amino acid tagging (BONCAT) and established that exercise exacerbated age-associated protein patterns through a dysregulated transforming growth factor (TGF)-β, Ras/MAPK/PI3Akt, and JAK/STAT pathways. Testing causality, we found that an inhibitor of TGF-β (Alk5 inhibitor, A5i) in combination with the age-diminished peptide oxytocin, previously shown to rejuvenate muscle and brain in sedentary animals, allowed aged mice to exercise without pathologies of skeletal and heart muscles and youthfully restored their de novo proteomes.

Podocytes are highly specialized glomerular visceral epithelial cells critical for maintaining the structure and function of the glomerular filtration barrier. These cells adhere to the glomerular basement membrane (GBM) and envelop the outer surfaces of the glomerular capillaries to prevent protein leakage during blood ultrafiltration. The GBM is a dense network of extracellular matrix composed of type IV collagen, laminin, nidogen, and heparan sulfate proteoglycans. In this study, we investigated the protective effect of a heparanase inhibitor on puromycin aminonucleoside (PAN)-induced podocyte injury. Our results demonstrate that PAN treatment significantly disrupted the cytoskeletal architecture of cultured podocytes, reducing the formation of focal adhesions and stress fibers. Interdigitating intercellular junctions were replaced by dot-like structures with accumulated filamentous actin. Co-treatment with the heparanase inhibitor PI-88 effectively prevented these PAN-induced cytoskeletal abnormalities. Furthermore, a BSA filtration assay revealed that PI-88 attenuated PAN-induced increases in podocyte monolayer permeability. Taken together, our findings suggest that heparanase inhibition protects against podocyte injury and may represent a potential therapeutic strategy for glomerular diseases.

RETRACTION: E. Nourmohammadi, H. Khoshdel–Sarkarizi, R. Nedaeinia, H. R. Sadeghnia, L. Hasanzadeh, M. Darroudi and R. K. Oskuee, “Evaluation of Anticancer Effects of Cerium Oxide Nanoparticles on Mouse Fibrosarcoma Cell Line,” Journal of Cellular Physiology 234, no. 4 (2019): 4987–4996, https://doi.org/10.1002/jcp.27303.

The above article, published online on 06 September 2018 in Wiley Online Library (wileyonlinelibrary.com), has been retracted by agreement between the journal Editor-in-Chief, Robert Heath; and Wiley Periodicals LLC. The retraction has been agreed upon following an investigation into concerns raised by a third party. The investigation revealed duplication between the L929-Control flow cytometry plot and the L929-Conc30 plot shown in Figure 7b. Furthermore, duplications were identified between the Conc 30 BAX western blot band presented in Figures 9a and the Control BAX western blot band in Figure 9b. These two figures represent different cell lines. An additional duplication was observed between the Control BCL2 band and the Conc 60 BAX band in Figure 9a. The authors provided an explanation and some data, but this was not deemed sufficient. The editors consider the results and conclusions from this study to be substantially compromised. The authors disagree with the retraction.

RETRACTION: W. Zhang, Y. Bi, Y. Wang, M. Wang, D. Li, S. Cheng, J. Jin, T. Li, B. Li, and Y. Zhang, “Nanos2 Promotes Differentiation of Male Germ Cells Basing on the Negative Regulation of Foxd3 and the Treatment of 5-Azadc and TSA,” Journal of Cellular Physiology, 234, no. 4 (2019): 3762–3774, https://doi.org/10.1002/jcp.27139.

The above article, published online on 26 August 2018 in Wiley Online Library (wileyonlinelibrary.com), has been retracted by agreement between the authors, the journal Editor-in-Chief, Robert Heath; and Wiley Periodicals LLC. The retraction has been agreed due to concerns raised by third parties. Specifically, it was brought to the journal's attention that Nanos2 has not been identified in Gallus gallus. Further investigation confirmed that the gene examined in the article was actually Nos2, which encodes nitric oxide synthase 2 (NOS2), a protein with different and unrelated functions from those proposed in the study. Author B. Li stated that she did not directly participate in the experiments conducted for the study and was unaware of its submission. The corresponding author Y. Zhang, on behalf of the remaining co-authors, stated that they mistakenly assumed Nos2, which serves as an alias for Nanos2 in other species, to be homologous to Nanos2 in chickens. This misidentification undermines the study's rationale, methodology, and conclusions. Accordingly, the article is retracted.

RETRACTION: Mansoori, B., A. Mohammadi, S. Naghizadeh, et al. 2020. “MiR-330 Suppresses EMT and Induces Apoptosis by Downregulating HMGA2 in Human Colorectal Cancer,” Journal of Cellular Physiology 235, no. 2: 920–931. https://doi.org/10.1002/jcp.29007.

The above article, published online on June 26, 2019 in Wiley Online Library (wileyonlinelibrary.com), has been retracted by agreement between the journal Editor-in-Chief, Robert Heath; and Wiley Periodicals LLC. The retraction has been agreed upon following an investigation by the publisher in response to concerns raised by third parties. Several flaws and inconsistencies have been identified between the described methodology and the presented results. Furthermore, the study's rationale and conclusions drawn are not supported by either the existing literature or the data extracted from The Cancer Genome Atlas (TCGA). Accordingly, the article is retracted as the editors consider its conclusions to be invalid. The authors have been informed of the decision of retraction but not available for a final confirmation.