目的：有报告称，金属会改变氧化还原活性金属和氧化还原非活性金属的氧化状态，同时诱发氧化应激。在水生生态系统中，金属污染被认为是严重的污染物和生物累积，尤其是涉及水生海产品时，会对人类造成危害。蓝游蟹（Portunus pelagicus）是泰国非常受欢迎的海鲜蟹种。其肉质部分和肝胰脏（HP）连同生殖腺都被食用，需求量很大。因此，本研究旨在调查鳗鲡体内镉（Cd）和铅（Pb）的生物累积以及组织氧化反应：方法：从 Trang 省沿岸的小型渔户处获得 67 份中上层鲈鱼样本。评估了镉和铅的生物累积以及鳃、肌肉和 HP + 性腺的氧化反应：结果：镉和铅在鳃和生殖腺中的积累水平最高，其次是鳃，然后是肌肉，这表明镉和铅在鳃和生殖腺中具有较高的亲和力。镉和铅在鳃和肌肉组织中显著激活超氧化物歧化酶（SOD）的活性，而铅仅在鳃和生殖腺中显著激活SOD的活性。只有镉在鳃组织中的积累才显著激活了脂质过氧化反应，丙二醛水平也表明了这一点：本研究表明，中上层鲈鱼对金属积累引起的组织氧化反应表现出 "适应阶段"。此外，本文提供的数据进一步表明，只食用肉的部分并去除HP和生殖腺可减少人类接触金属毒性的机会。
{"title":"Oxidative response to cadmium and lead accumulations in the tissues of blue swimming crabs Portunus pelagicus from the Trang Province coastline, Southern Thailand","authors":"Kanjana Imsilp, Phanwimol Tanhan, Niyada Lansubsakul, Wachiryah Thong-asa","doi":"10.1002/aah.10229","DOIUrl":"10.1002/aah.10229","url":null,"abstract":"<p>\u0000 \u0000 </p>","PeriodicalId":15235,"journal":{"name":"Journal of aquatic animal health","volume":"36 3","pages":"265-274"},"PeriodicalIF":1.5,"publicationDate":"2024-08-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141995769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"A perspective: Molecular detections of new agents in finfish—Interpreting biological significance for fish health management: Response to comment","authors":"Theodore R. Meyers, Nora Hickey","doi":"10.1002/aah.10222","DOIUrl":"10.1002/aah.10222","url":null,"abstract":"<p>\u0000 \u0000 </p>","PeriodicalId":15235,"journal":{"name":"Journal of aquatic animal health","volume":"36 3","pages":"231-238"},"PeriodicalIF":1.5,"publicationDate":"2024-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/aah.10222","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141751761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Gideon Mordecai, Emiliano Di Cicco, Christoph Deeg, Andrew Bateman, Amy Teffer, Kristi Miller
<p>Emerging infectious diseases in wildlife populations—and fish in particular—have drawn increasing attention in recent decades (Daszak et al. <span>2000</span>; Alexander and McNutt <span>2010</span>; Miller et al. <span>2014</span>; Tompkins et al. <span>2015</span>; Krkošek <span>2017</span>), especially as climate change and biodiversity loss present a shifting context that will almost certainly affect disease dynamics across taxa (Daszak et al. <span>2000</span>; Miller et al. <span>2014</span>). Management of infectious disease is especially important when populations of conservation concern are potentially impacted. In this context, healthy and transparent discussion and debate about how scientists, veterinary professionals, conservationists, policymakers, and regulators approach emerging infectious diseases in wildlife are much needed.</p><p>Primarily, we are concerned that the perspective offered by Meyers and Hickey (<span>2022</span>) does not adequately consider precautionary management or the conservation status of wildlife populations. Additionally, although Meyers and Hickey recognize some of the potential power of molecular technologies (e.g., next-generation DNA sequencing and quantitative polymerase chain reaction [qPCR]) in finfish disease research, they make several statements and present misconceptions that require clarification, including instances in which relevant details of our own work (Mordecai et al. <span>2019</span>) are misrepresented or inappropriately discounted.</p><p>Drawing on consultation with a confidential group of fish health professionals and administrators, Meyers and Hickey propose that upon molecular detection of a novel infectious agent in fish or shellfish (or a known agent in a new host or setting), a decision pathway should be followed to avoid wasting resources prior to any changes to regulatory policy. Their proposed pathway suggests that an infectious agent should be detected with multiple assays, should be shown to replicate in its host, should be associated with clinical disease and mortality, and should fulfill Koch's postulates of disease causality. In theory, this series of scientific confirmations, which are to be addressed prior to enacting changes in surveillance or regulation, would inform a conceptually valid decision-making pathway. While we welcome guidelines to facilitate management of emerging infectious disease (World Organisation for Animal Health <span>2021</span>), we argue that the criteria proposed by Meyers and Hickey fail to capture the complex reality of managing emerging infectious diseases in wildlife populations, particularly those of conservation concern, and are not suitable in practice.</p><p>Here lies perhaps the most salient point of what we see as our disagreement with Meyers and Hickey: while the decision pathway that they propose seems (to us) to err on the side of avoiding false positives (where infectious agent risks are truly low and changes to policy or mana
{"title":"Comment on a perspective: Molecular detections of new agents in finfish—Interpreting biological significance for fish health management","authors":"Gideon Mordecai, Emiliano Di Cicco, Christoph Deeg, Andrew Bateman, Amy Teffer, Kristi Miller","doi":"10.1002/aah.10221","DOIUrl":"10.1002/aah.10221","url":null,"abstract":"<p>Emerging infectious diseases in wildlife populations—and fish in particular—have drawn increasing attention in recent decades (Daszak et al. <span>2000</span>; Alexander and McNutt <span>2010</span>; Miller et al. <span>2014</span>; Tompkins et al. <span>2015</span>; Krkošek <span>2017</span>), especially as climate change and biodiversity loss present a shifting context that will almost certainly affect disease dynamics across taxa (Daszak et al. <span>2000</span>; Miller et al. <span>2014</span>). Management of infectious disease is especially important when populations of conservation concern are potentially impacted. In this context, healthy and transparent discussion and debate about how scientists, veterinary professionals, conservationists, policymakers, and regulators approach emerging infectious diseases in wildlife are much needed.</p><p>Primarily, we are concerned that the perspective offered by Meyers and Hickey (<span>2022</span>) does not adequately consider precautionary management or the conservation status of wildlife populations. Additionally, although Meyers and Hickey recognize some of the potential power of molecular technologies (e.g., next-generation DNA sequencing and quantitative polymerase chain reaction [qPCR]) in finfish disease research, they make several statements and present misconceptions that require clarification, including instances in which relevant details of our own work (Mordecai et al. <span>2019</span>) are misrepresented or inappropriately discounted.</p><p>Drawing on consultation with a confidential group of fish health professionals and administrators, Meyers and Hickey propose that upon molecular detection of a novel infectious agent in fish or shellfish (or a known agent in a new host or setting), a decision pathway should be followed to avoid wasting resources prior to any changes to regulatory policy. Their proposed pathway suggests that an infectious agent should be detected with multiple assays, should be shown to replicate in its host, should be associated with clinical disease and mortality, and should fulfill Koch's postulates of disease causality. In theory, this series of scientific confirmations, which are to be addressed prior to enacting changes in surveillance or regulation, would inform a conceptually valid decision-making pathway. While we welcome guidelines to facilitate management of emerging infectious disease (World Organisation for Animal Health <span>2021</span>), we argue that the criteria proposed by Meyers and Hickey fail to capture the complex reality of managing emerging infectious diseases in wildlife populations, particularly those of conservation concern, and are not suitable in practice.</p><p>Here lies perhaps the most salient point of what we see as our disagreement with Meyers and Hickey: while the decision pathway that they propose seems (to us) to err on the side of avoiding false positives (where infectious agent risks are truly low and changes to policy or mana","PeriodicalId":15235,"journal":{"name":"Journal of aquatic animal health","volume":"36 3","pages":"220-230"},"PeriodicalIF":1.5,"publicationDate":"2024-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/aah.10221","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141751762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Long-term effects of iopamidol as a contrast medium for computed tomography in Cloudy Catsharks Scyliorhinus torazame","authors":"Takaomi Ito, Masaru Furuya, Toshiyuki Tanaka, Yusuke Yoshii, Mikito Murata, Kazumi Sasai","doi":"10.1002/aah.10219","DOIUrl":"10.1002/aah.10219","url":null,"abstract":"<p>\u0000 \u0000 </p>","PeriodicalId":15235,"journal":{"name":"Journal of aquatic animal health","volume":"36 3","pages":"239-249"},"PeriodicalIF":1.5,"publicationDate":"2024-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140634253","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
目的2023年5月，在泰国巴真武里府浮游网箱养殖的红罗非鱼（Oreochromis sp.）出现急性死亡，并伴有类似链球菌感染的临床症状。方法从病鱼的大脑和肾脏中分离出细菌后，使用基质辅助激光解吸/电离飞行时间质谱和 VITEK 2 系统进行鉴定。采用 16S 核糖体 RNA（rRNA）基因测序和系统进化分析来确认细菌种类。还进行了抗菌药敏感性测试。结果从 20 份鱼类样本中鉴定出 L. garvieae（n = 18 个分离物）和无乳链球菌（n = 2 个分离物）。16S rRNA 基因的系统发生树显示，泰国分离出的 L. garvieae 或 S. agalactiae 与来自洲际地区的参考鱼类分离物聚集在一起。我们的分离物对喹诺酮类药物有抗药性，但对其他抗菌药物敏感。组织病理学变化显示出严重的败血症状况，与 S. agalactiae 感染的鱼相比，L. garvieae 感染的鱼有更多的侵袭性病变，尤其是心脏和肝脏。
{"title":"Characterization of Lactococcus garvieae and Streptococcus agalactiae in cultured red tilapia Oreochromis sp. in Thailand","authors":"Sasibha Jantrakajorn, Watcharapol Suyapoh, Janenuj Wongtavatchai","doi":"10.1002/aah.10217","DOIUrl":"10.1002/aah.10217","url":null,"abstract":"<p>\u0000 \u0000 </p>","PeriodicalId":15235,"journal":{"name":"Journal of aquatic animal health","volume":"36 2","pages":"192-202"},"PeriodicalIF":1.2,"publicationDate":"2024-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140630655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Wade Cavender, Christine Swan, Skylar Wolf, Danielle Van Vliet, Alison Aceves Johnson, Anna Forest, Robert Shields, Thomas Loch, Christopher Knupp, John Drennan, Gavin Glenney, Sascha L. Hallett, Joe Marcino, Aimee Reed
{"title":"Effect of tea polyphenol–trehalose complex coating solutions on physiological stress and flesh quality of marine-cultured Turbot Scophthalmus maximus during waterless transport","authors":"Jie Cao, Meijie Guo, Weiqiang Qiu, Jun Mei, Jing Xie","doi":"10.1002/aah.10213","DOIUrl":"10.1002/aah.10213","url":null,"abstract":"<p>\u0000 \u0000 </p>","PeriodicalId":15235,"journal":{"name":"Journal of aquatic animal health","volume":"36 2","pages":"151-163"},"PeriodicalIF":1.2,"publicationDate":"2024-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140101643","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Karima A. Bakry, Walaa F. A. Emeish, Hamdy M. Embark, Ahmad A. Elkamel, Haitham H. Mohammed
Objective
During Egypt's hot summer season, Aeromonas veronii infection causes catastrophic mortality on Nile Tilapia Oreochromis niloticus farms. Egypt is ranked first in aquaculture production in Africa, sixth in aquaculture production worldwide, and third in global tilapia production. This study aimed to investigate, at the molecular level, the early innate immune responses of Nile Tilapia to experimental A. veronii infection.
Methods
The relative gene expression, co-expression clustering, and correlation of four selected immune genes were studied by quantitative real-time polymerase chain reaction in four organs (spleen, liver, gills, and intestine) for up to 72 h after a waterborne A. veronii challenge. The four genes studied were nucleotide-binding oligomerization domain 1 (NOD1), lipopolysaccharide-binding protein (LBP), natural killer-lysin (NKL), and interleukin-1 beta (IL-1β).
Result
The four genes showed significant transcriptional upregulation in response to infection. At 72 h postchallenge, the highest NOD1 and IL-1β expression levels were recorded in the spleen, whereas the highest LBP and NKL expression levels were found in the gills. Pairwise distances of the data points and the hierarchical relationship showed that NOD1 clustered with IL-1β, whereas LBP clustered with NKL; both genes within each cluster showed a significant positive expression correlation. Tissue clustering indicated that the responses of only the gill and intestine exhibited a significant positive correlation.
Conclusion
The results suggest that NOD1, LBP, NKL, and IL-1β genes play pivotal roles in the early innate immune response of Nile Tilapia to A. veronii infection, and the postinfection expression profile trends of these genes imply tissue-/organ-specific responses and synchronized co-regulation.
{"title":"Expression profiles of four Nile Tilapia innate immune genes during early stages of Aeromonas veronii infection","authors":"Karima A. Bakry, Walaa F. A. Emeish, Hamdy M. Embark, Ahmad A. Elkamel, Haitham H. Mohammed","doi":"10.1002/aah.10214","DOIUrl":"10.1002/aah.10214","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objective</h3>\u0000 \u0000 <p>During Egypt's hot summer season, <i>Aeromonas veronii</i> infection causes catastrophic mortality on Nile Tilapia <i>Oreochromis niloticus</i> farms. Egypt is ranked first in aquaculture production in Africa, sixth in aquaculture production worldwide, and third in global tilapia production. This study aimed to investigate, at the molecular level, the early innate immune responses of Nile Tilapia to experimental <i>A. veronii</i> infection.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>The relative gene expression, co-expression clustering, and correlation of four selected immune genes were studied by quantitative real-time polymerase chain reaction in four organs (spleen, liver, gills, and intestine) for up to 72 h after a waterborne <i>A. veronii</i> challenge. The four genes studied were nucleotide-binding oligomerization domain 1 (<i>NOD1</i>), lipopolysaccharide-binding protein (<i>LBP</i>), natural killer-lysin (<i>NKL</i>), and interleukin-1 beta (<i>IL-1</i>β).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Result</h3>\u0000 \u0000 <p>The four genes showed significant transcriptional upregulation in response to infection. At 72 h postchallenge, the highest <i>NOD1</i> and <i>IL-1</i>β expression levels were recorded in the spleen, whereas the highest <i>LBP</i> and <i>NKL</i> expression levels were found in the gills. Pairwise distances of the data points and the hierarchical relationship showed that <i>NOD1</i> clustered with <i>IL-1</i>β, whereas <i>LBP</i> clustered with <i>NKL</i>; both genes within each cluster showed a significant positive expression correlation. Tissue clustering indicated that the responses of only the gill and intestine exhibited a significant positive correlation.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>The results suggest that <i>NOD1</i>, <i>LBP</i>, <i>NKL,</i> and <i>IL-1</i>β genes play pivotal roles in the early innate immune response of Nile Tilapia to <i>A. veronii</i> infection, and the postinfection expression profile trends of these genes imply tissue-/organ-specific responses and synchronized co-regulation.</p>\u0000 </section>\u0000 </div>","PeriodicalId":15235,"journal":{"name":"Journal of aquatic animal health","volume":"36 2","pages":"164-180"},"PeriodicalIF":1.2,"publicationDate":"2024-02-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/aah.10214","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139996328","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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