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Identification and analysis of pathogenic bacteria causing outbreaks in Indian major carp aquaculture of Tripura 印度特里普拉邦主要鲤鱼养殖场爆发病原菌的鉴定与分析。
IF 1.2 4区 农林科学 Q3 FISHERIES
Journal of aquatic animal health
Pub Date : 2023-08-16 DOI: 10.1002/aah.10198
C. Laltlanmawia, Himadri Saha, Lija Ghosh, Ratan Kumar Saha, Supratim Malla

目的:本研究的目的是调查印度特里普拉(Tripura)养殖系统中印度主要鲤鱼的细菌性疾病暴发，并确定与这些暴发相关的细菌种类。方法:在特里普拉邦的8个区进行了为期3年的监测，期间记录了9起细菌性疾病暴发。采用表型分析、分子分析(16S核糖体RNA基因)和系统发育分析等方法，对从病鲤中分离的14株细菌进行了鉴定。进行体外致病性研究以评估分离细菌的潜在致病性。结果:所选分离菌株初步鉴定为气单胞菌属(9株)、不动杆菌属(1株)、柠檬酸杆菌属(3株)和假单胞菌属(1株)。分子和系统发育分析证实了分离细菌的种类，包括江氏气单胞菌(菌株COF_AHE09和COF_AHE61)、维罗氏气单胞菌(菌株COF_AHE13、COF_AHE52、COF_AHE55、COF_AHE56和COF_AHE62)、嗜水气单胞菌(菌株COF_AHE51和COF_AHE58)、皮氏不动杆菌(菌株COF_AHE14)、弗氏柠檬酸杆菌(菌株COF_AHE20、COF_AHE57和COF_AHE59)和铜绿假单胞菌(菌株COF_AHE54)。病鱼表现为嗜睡、皮肤出血、溃疡、翅尾腐烂、突出眼、腹部肿胀、鳞片脱落、皮肤变色等行为和临床表现，提示细菌性败血症。体外致病性研究强调了这些细菌在疾病发展中的潜在作用，特别是在环境胁迫下。结论:本研究为了解与特里普拉邦养殖系统中印度主要鲤鱼细菌性疾病暴发相关的细菌物种多样性提供了有价值的见解。这是第一次全面的调查，有助于我们了解印度主要鲤鱼的细菌感染。
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引用次数: 0
Effects of haloperidol on peripheral erythrocytes and brain neurotransmitter levels of juvenile African Sharptooth Catfish Clarias gariepinus 氟哌啶醇对幼年非洲尖牙鲶鱼外周血红细胞和脑神经递质水平的影响。
IF 1.2 4区 农林科学 Q3 FISHERIES
Journal of aquatic animal health
Pub Date : 2023-07-28 DOI: 10.1002/aah.10195
Chike Obinna Chiejina, Ifeanyi Mulumba Ikeh, Florence A. Enebe, Ifeanyi Oscar Aguzie, Malachy Nwigwe Okechukwu Ajima, Daoud Ali, Gokhlesh Kumar, Christopher Didigwu Nwani

目的：研究氟哌啶醇对幼年非洲尖牙鲶鱼外周血红细胞及脑神经递质水平的影响。方法：青少年暴露于不同浓度的氟哌啶醇（0.12、0.24和0.48 mg/L）15 天，随后停药5天 天。在第1天、第5天、第10天和第15天以及5天停药期后，分析鱼的血液样本的突变变化，之后处死鱼。对大脑进行5-羟色胺能和多巴胺能分析。结果：鱼外周血中有微核形成，微核随着用药时间和浓度的增加而增加。该药物显著降低了血清素活性，但增加了多巴胺活性。然而，在5天的停药期后，一些研究参数从药物的影响中恢复。结论：氟哌啶醇对鱼类具有毒性，应注意其在环境中的使用，避免对鱼类等非目标物种产生不利影响。
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引用次数: 0
Metal–amino acid complexes (Zn, Se, Cu, Fe, and Mn) enhance immune response, antioxidant capacity, liver function enzymes, and expression of cytokine genes in Nile Tilapia reared under field conditions 金属-氨基酸复合物(锌、硒、铜、铁和锰)可增强尼罗罗非鱼的免疫反应、抗氧化能力、肝功能酶和细胞因子基因的表达。
IF 1.2 4区 农林科学 Q3 FISHERIES
Journal of aquatic animal health
Pub Date : 2023-07-28 DOI: 10.1002/aah.10194
Abdel-Fattah M. El-Sayed, Claudia Figueiredo-Silva, Salma M. S. Zeid, Sarah O. Makled

目的:研究了在野外条件下，用金属-氨基酸复合物(MAACs)部分或全部替代饲料中无机微量物质(ITM)对尼罗罗非鱼(Oreochromis niloticus)免疫应答、抗氧化能力、细胞因子基因表达和整体健康状况的影响。方法:将平均初始体重约为90 g -1的全雄性尼罗罗非鱼分成四组，以每半区60尾鱼(30尾m-3)的密度放养。每个试验区对养殖条件和试验饲料进行1周的驯化。定期监测水质参数，包括温度(°C)、pH、溶解氧(mg L-1)和总氨(mg L-1)。maac的配方中含有与无机预混料中相同的矿物质浓度。金属-氨基酸复合物以0(对照)、25、50、75和100%的水平替代ITM预混料(表1)。将饲料饲喂笼养尼罗罗非鱼，每天两次，连续80天，每天饲喂量为其体重的3%。每10天称重一次，并重新调整每日口粮。在收获时，每个hapa的鱼被收集、计数和称重。每个hapa的5只鱼被快速麻醉，并用于化学和生理分析，包括抗氧化和免疫反应分析，肝功能分析和基因表达。结果:随着MAACs添加量的增加，溶菌酶、呼吸爆发酶(%)、替代补体酶(ACH50)、吞噬细胞、酚氧化酶、超氧化物歧化酶、谷胱甘肽过氧化物酶和肝功能酶的活性均有所提高，替代量达到50%。与对照组相比，添加maac的饲料显著上调了干扰素、肿瘤坏死因子、白细胞介素-1、血清碱性磷酸酶、综合微生物基因组、氯霉素乙酰转移酶和转化生长因子基因等细胞因子基因的表达。50% MAAC水平下，这些基因的表达量上调幅度最大，而随着饲粮MAAC水平的增加，这些基因的表达量下降到75%和100%。二次回归分析表明，在田间条件下，池内养殖尼罗罗非鱼的免疫和氧化应激反应、基因表达和整体健康状况的最大促进需要60-65%的MAAC。结论:用MAAC预混料替代ITM预混料可增强尼罗罗非鱼的免疫和抗氧化反应。
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引用次数: 0
Identification and pathogenicity of multidrug-resistant Elizabethkingia miricola isolated from farmed American bullfrogs Rana catesbeiana in China with in vitro screening of herbal antimicrobial agents 中国养殖美国牛蛙(Rana catesbeiana)多药耐药菌株的鉴定和致病性及中草药抗菌药物的体外筛选
IF 1.2 4区 农林科学 Q3 FISHERIES
Journal of aquatic animal health
Pub Date : 2023-06-18 DOI: 10.1002/aah.10185
Fangyuan Liu, Jiahao Hou, Fang Yu, Zemao Gu, Ruixue Hu

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引用次数: 0
Susceptibility of Pallid Sturgeon to viral hemorrhagic septicemia virus genotype IVb 白鲟对病毒出血性败血症病毒基因型IVb的易感性
IF 1.2 4区 农林科学 Q3 FISHERIES
Journal of aquatic animal health
Pub Date : 2023-05-26 DOI: 10.1002/aah.10181
Lacey R. Hopper, Jolene A. Glenn, Elizabeth MacConnell, James R. Winton, Eveline J. Emmenegger

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引用次数: 0
Histopathological alterations in the gonads of wild white clams Dosinia ponderosa inhabiting a former copper mine locality in the Gulf of California 加利福尼亚湾原铜矿野生白蛤性腺的组织病理学改变
IF 1.2 4区 农林科学 Q3 FISHERIES
Journal of aquatic animal health
Pub Date : 2023-05-26 DOI: 10.1002/aah.10184
Jessica F. Eraso-Ordoñez, Josué Alonso Yee-Duarte, Nefertiti Taydé Roldán-Wong, Manuel García-Ulloa, Andrés M. Góngora-Gómez, Marcial Arellano-Martínez

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引用次数: 0
Observations of fin injury closure in Great Hammerheads and implications for the use of fin-mounted geolocators 双髻鲨鳍伤闭合的观察及其对安装在鳍上的地理定位器使用的意义
IF 1.2 4区 农林科学 Q3 FISHERIES
Journal of aquatic animal health
Pub Date : 2023-05-24 DOI: 10.1002/aah.10178
Vital Heim, R. Dean Grubbs, Matthew J. Smukall, Bryan S. Frazier, John K. Carlson, Tristan L. Guttridge

Objective

Sharks face a high risk of injuries throughout all life stages and are therefore expected to show a good wound closure capacity.

Methods

Here, the wound closure of one major injury and one minor injury to the first dorsal fins of two free-ranging, mature female Great Hammerheads Sphyrna mokarran is described macroscopically.

Result

The sharks showed complete wound closure of single, clean-cut lacerations measuring 24.2 and 11.6 cm in length after an estimated 323 and 138 days. These estimates were based on the observed closure rate and visual confirmation of a complete wound closure upon multiple resightings of the same individuals. Additionally, the posterior lateral displacement of fin-mounted geolocators within the fin and outside of the fin without causing external damage was documented in three additional Great Hammerheads.

Conclusion

These observations supplement findings about wound closure capabilities in elasmobranchs. The documented geolocator displacement furthers the discussion about the safe use of these geolocators to track shark movements but also has implications for future tagging studies.

鲨鱼在整个生命阶段都面临着很高的受伤风险，因此预计会表现出良好的伤口愈合能力。方法对两只自由放养的成年雌性大锤头鲨(Great Hammerheads sphyna mokarran)第一背鳍1个大伤和1个小伤的伤口愈合进行宏观描述。结果在323天和138天后，鲨鱼的伤口完全愈合，伤口长度分别为24.2和11.6厘米。这些估计是基于观察到的愈合率和对同一个体进行多次复查后伤口完全愈合的视觉确认。此外，在另外三只大锤头鲨中，安装在鳍内和鳍外的定位器的后外侧位移没有造成外部损伤。结论这些观察结果补充了板鳃动物伤口愈合能力的研究结果。记录的地理定位器位移进一步讨论了安全使用这些地理定位器来跟踪鲨鱼的运动，但也对未来的标记研究有影响。
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引用次数: 0
Characterization of two tripartite motif-containing genes from Asian Seabass Lates calcarifer and their expression in response to virus infection and microbial molecular motifs 亚洲鲈鱼两个三重基序基因的特征及其在病毒感染和微生物分子基序反应中的表达。
IF 1.2 4区 农林科学 Q3 FISHERIES
Journal of aquatic animal health
Pub Date : 2023-05-04 DOI: 10.1002/aah.10187
Krishnapriya Raji Sathyan, Avinash Premraj, Sajeevan Thavarool Puthiyedathu

目的：我们从亚洲鲈鱼Lates calcarifer中鉴定了两个三重基序（TRIM）基因，LcTRIM21和LcTRIM39，并用微生物病原体相关的分子模式检测了它们对实验性倍他野病毒感染和刺激的反应。方法：从亚洲鲈鱼中鉴定、克隆和测序编码LcTRIM21和LcTRIM39的基因。我们使用各种生物信息学工具分析了该序列，以确定蛋白质结构、定位并建立系统发育树。通过使用定量实时PCR，我们分析了LcTRIM21和LcTRIM39基因对倍他野病毒攻击的表达谱，以及与分子病原体相关的分子模式，如poly（I:C）和Zymosan A。还检测了这些基因在健康动物中的组织分布模式。结果：克隆了亚洲鲈鱼TRIM基因的同源物LcTRIM21和LcTRIM39，它们都编码547个氨基酸的蛋白质。LcTRIM21的等电点为6.32，分子量为62.11千道尔顿，而LcTRIM39的等电点将为5.57，分子量则为62.11千道尔顿。通过计算机蛋白质定位预测LcTRIM21和LcTRIM39同源物定位在细胞质中。从结构上讲，这两种蛋白质都含有一个N端非常有趣的新基因（RING）锌指结构域、B盒结构域、卷曲螺旋结构域和C端PRY/SPRY结构域。所检查的大多数组织和器官显示LcTRIM21和LcTRIM39的组成型表达。在poly（I:C）攻击或红斑石斑鱼神经坏死病毒感染后，LcTRIM21和LcTRIM39 mRNA表达显著上调，表明它们可能对鱼类病毒发挥关键的抗病毒作用。LcTRIM21和LcTRIM39的表达也通过给予葡聚糖Zymosan A而上调。结论：含有TRIM的基因是一种E3泛素连接酶，通过蛋白酶体介导的泛素化靶向病毒蛋白而表现出抗病毒活性。TRIM蛋白可用于发现抗病毒药物和对抗病毒性神经坏死等疾病的策略，这些疾病威胁着鲈鱼养殖。
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引用次数: 1
Efficacy of hydrogen peroxide to reduce Gyrodactylus species infestation density on four fish species 过氧化氢降低旋毛虫对四种鱼类侵害密度的效果
IF 1.2 4区 农林科学 Q3 FISHERIES
Journal of aquatic animal health
Pub Date : 2023-04-11 DOI: 10.1002/aah.10179
Maren T. Tuttle-Lau, Eric M. Leis, Aaron R. Cupp, Laura L. Peterman, Jillian L. Hebert, Richard A. Erickson, Susan M. Schleis, Mark P. Gaikowski

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引用次数: 1
Tissue and plasma enzyme activities and chemical analytes in Golden Trevally from a public aquarium 来自公共水族馆的Golden Trevaly的组织和血浆酶活性以及化学分析。
IF 1.2 4区 农林科学 Q3 FISHERIES
Journal of aquatic animal health
Pub Date : 2023-03-20 DOI: 10.1002/aah.10183
Kathryn Rapp, Sean Perry, Alexa Delaune, Justin Stilwell

目的：水生物种，特别是鱼类的兽医护理因缺乏对其独特生理学的了解而受到限制。在血浆中测量的组织酶活性用于评估特定器官的功能和潜在损伤，并跟踪活体动物的疾病进展。这项研究的目的是确定健康的金纹Gnathanodon specious的来源组织和特定酶的血浆浓度。我们假设酶会表现出组织特异性的热带性，与其他组织相比，在一个或多个组织中具有更高的活性。方法：从一个公共水族馆群体中随机选择6条鱼，获取尸检血样。然后对这些鱼实施安乐死，并通过大体尸检采集组织样本。对每条鱼的血浆和10个组织样本进行了6种酶活性和2种化学分析。结果：酶活性表现出显著的器官特异性。骨骼肌中天冬氨酸氨基转移酶、乳酸脱氢酶和肌酸激酶水平最高，性腺中的水平不同。肾脏、脾脏和肝脏的碱性磷酸酶水平最高。丙氨酸氨基转移酶水平对肝脏具有高度特异性。γ-谷氨酰转移酶仅在肾脏和血浆中检测到。结论：这项工作为Golden Trevaly建立了组织酶的基线来源，这将有助于临床医生对血液化学的诊断解释，并改善对这种研究不足的鱼类的兽医护理。
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引用次数: 1
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