Taylor Abraham, Zeinab Yazdi, Eric Littman, Khalid Shahin, Taylor I. Heckman, Eva Marie Quijano Cardé, Diem Thu Nguyen, Ruixue Hu, Mark Adkison, Tresa Veek, Kavery Mukkatira, Christine Richey, Kevin Kwak, Haitham H. Mohammed, Cesar Ortega, Ruben Avendaño-Herrera, William Keleher, Véronique LePage, Ian Gardner, Timothy J. Welch, Esteban Soto
Objective
The first objective of the study aimed to detect the presence of Lactococcus petauri, L. garvieae, and L. formosensis in fish (n = 359) and environmental (n = 161) samples from four lakes near an affected fish farm in California during an outbreak in 2020. The second objective was to compare the virulence of the Lactococcus spp. in Rainbow Trout Oncorhynchus mykiss and Largemouth Bass Micropterus salmoides.
Methods
Standard bacterial culture methods were used to isolate Lactococcus spp. from brain and posterior kidney of sampled fish from the four lakes. Quantitative PCR (qPCR) was utilized to detect Lactococcus spp. DNA in fish tissues and environmental samples from the four lakes. Laboratory controlled challenges were conducted by injecting fish intracoelomically with representative isolates of L. petauri (n = 17), L. garvieae (n = 2), or L. formosensis (n = 4), and monitored for 14 days postchallenge (dpc).
Result
Lactococcus garvieae was isolated from the brains of two Largemouth Bass in one of the lakes. Lactococcus spp. were detected in 14 fish (8 Bluegills Lepomis macrochirus and 6 Largemouth Bass) from 3 out of the 4 lakes using a qPCR assay. Of the collected environmental samples, all 4 lakes tested positive for Lactococcus spp. in the soil samples, while 2 of the 4 lakes tested positive in the water samples through qPCR. Challenged Largemouth Bass did not show any signs of infection postinjection throughout the challenge period. Rainbow Trout infected with L. petauri showed clinical signs within 3 dpc and presented a significantly higher cumulative mortality (62.4%; p < 0.0001) at 14 dpc when compared to L. garvieae (0%) and L. formosensis (7.5%) treatments.
Conclusion
The study suggests that qPCR can be used for environmental DNA monitoring of Lactococcus spp. and demonstrates virulence diversity between the etiological agents of piscine lactococcosis.
{"title":"Detection and virulence of Lactococcus garvieae and L. petauri from four lakes in southern California","authors":"Taylor Abraham, Zeinab Yazdi, Eric Littman, Khalid Shahin, Taylor I. Heckman, Eva Marie Quijano Cardé, Diem Thu Nguyen, Ruixue Hu, Mark Adkison, Tresa Veek, Kavery Mukkatira, Christine Richey, Kevin Kwak, Haitham H. Mohammed, Cesar Ortega, Ruben Avendaño-Herrera, William Keleher, Véronique LePage, Ian Gardner, Timothy J. Welch, Esteban Soto","doi":"10.1002/aah.10188","DOIUrl":"10.1002/aah.10188","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objective</h3>\u0000 \u0000 <p>The first objective of the study aimed to detect the presence of <i>Lactococcus petauri</i>, <i>L. garvieae</i>, and <i>L. formosensis</i> in fish (<i>n</i> = 359) and environmental (<i>n</i> = 161) samples from four lakes near an affected fish farm in California during an outbreak in 2020. The second objective was to compare the virulence of the <i>Lactococcus</i> spp. in Rainbow Trout <i>Oncorhynchus mykiss</i> and Largemouth Bass <i>Micropterus salmoides</i>.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Standard bacterial culture methods were used to isolate <i>Lactococcus</i> spp. from brain and posterior kidney of sampled fish from the four lakes. Quantitative PCR (qPCR) was utilized to detect <i>Lactococcus</i> spp. DNA in fish tissues and environmental samples from the four lakes. Laboratory controlled challenges were conducted by injecting fish intracoelomically with representative isolates of <i>L. petauri</i> (<i>n</i> = 17), <i>L. garvieae</i> (<i>n</i> = 2), or <i>L. formosensis</i> (<i>n</i> = 4), and monitored for 14 days postchallenge (dpc).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Result</h3>\u0000 \u0000 <p><i>Lactococcus garvieae</i> was isolated from the brains of two Largemouth Bass in one of the lakes. <i>Lactococcus</i> spp. were detected in 14 fish (8 Bluegills <i>Lepomis macrochirus</i> and 6 Largemouth Bass) from 3 out of the 4 lakes using a qPCR assay. Of the collected environmental samples, all 4 lakes tested positive for <i>Lactococcus</i> spp. in the soil samples, while 2 of the 4 lakes tested positive in the water samples through qPCR. Challenged Largemouth Bass did not show any signs of infection postinjection throughout the challenge period. Rainbow Trout infected with <i>L. petauri</i> showed clinical signs within 3 dpc and presented a significantly higher cumulative mortality (62.4%; <i>p</i> < 0.0001) at 14 dpc when compared to <i>L. garvieae</i> (0%) and <i>L. formosensis</i> (7.5%) treatments.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>The study suggests that qPCR can be used for environmental DNA monitoring of <i>Lactococcus</i> spp. and demonstrates virulence diversity between the etiological agents of piscine lactococcosis.</p>\u0000 </section>\u0000 </div>","PeriodicalId":15235,"journal":{"name":"Journal of aquatic animal health","volume":"35 3","pages":"187-198"},"PeriodicalIF":1.2,"publicationDate":"2023-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/aah.10188","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41126502","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Modeling the importance of fish condition, overall health, and disease on the fecundity of White Perch in the Choptank River","authors":"Jacob T. Shaner, Reginal M. Harrell, John M. Jacobs, Lance T. Yonkos, Howard Townsend","doi":"10.1002/aah.10186","DOIUrl":"10.1002/aah.10186","url":null,"abstract":"<p>\u0000 \u0000 </p>","PeriodicalId":15235,"journal":{"name":"Journal of aquatic animal health","volume":"35 3","pages":"154-168"},"PeriodicalIF":1.2,"publicationDate":"2023-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10083128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Identification and analysis of pathogenic bacteria causing outbreaks in Indian major carp aquaculture of Tripura","authors":"C. Laltlanmawia, Himadri Saha, Lija Ghosh, Ratan Kumar Saha, Supratim Malla","doi":"10.1002/aah.10198","DOIUrl":"10.1002/aah.10198","url":null,"abstract":"<p>\u0000 \u0000 </p>","PeriodicalId":15235,"journal":{"name":"Journal of aquatic animal health","volume":"35 4","pages":"263-279"},"PeriodicalIF":1.2,"publicationDate":"2023-08-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10359913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abdel-Fattah M. El-Sayed, Claudia Figueiredo-Silva, Salma M. S. Zeid, Sarah O. Makled
目的:研究了在野外条件下，用金属-氨基酸复合物(MAACs)部分或全部替代饲料中无机微量物质(ITM)对尼罗罗非鱼(Oreochromis niloticus)免疫应答、抗氧化能力、细胞因子基因表达和整体健康状况的影响。方法:将平均初始体重约为90 g -1的全雄性尼罗罗非鱼分成四组，以每半区60尾鱼(30尾m-3)的密度放养。每个试验区对养殖条件和试验饲料进行1周的驯化。定期监测水质参数，包括温度(°C)、pH、溶解氧(mg L-1)和总氨(mg L-1)。maac的配方中含有与无机预混料中相同的矿物质浓度。金属-氨基酸复合物以0(对照)、25、50、75和100%的水平替代ITM预混料(表1)。将饲料饲喂笼养尼罗罗非鱼，每天两次，连续80天，每天饲喂量为其体重的3%。每10天称重一次，并重新调整每日口粮。在收获时，每个hapa的鱼被收集、计数和称重。每个hapa的5只鱼被快速麻醉，并用于化学和生理分析，包括抗氧化和免疫反应分析，肝功能分析和基因表达。结果:随着MAACs添加量的增加，溶菌酶、呼吸爆发酶(%)、替代补体酶(ACH50)、吞噬细胞、酚氧化酶、超氧化物歧化酶、谷胱甘肽过氧化物酶和肝功能酶的活性均有所提高，替代量达到50%。与对照组相比，添加maac的饲料显著上调了干扰素、肿瘤坏死因子、白细胞介素-1、血清碱性磷酸酶、综合微生物基因组、氯霉素乙酰转移酶和转化生长因子基因等细胞因子基因的表达。50% MAAC水平下，这些基因的表达量上调幅度最大，而随着饲粮MAAC水平的增加，这些基因的表达量下降到75%和100%。二次回归分析表明，在田间条件下，池内养殖尼罗罗非鱼的免疫和氧化应激反应、基因表达和整体健康状况的最大促进需要60-65%的MAAC。结论:用MAAC预混料替代ITM预混料可增强尼罗罗非鱼的免疫和抗氧化反应。
{"title":"Metal–amino acid complexes (Zn, Se, Cu, Fe, and Mn) enhance immune response, antioxidant capacity, liver function enzymes, and expression of cytokine genes in Nile Tilapia reared under field conditions","authors":"Abdel-Fattah M. El-Sayed, Claudia Figueiredo-Silva, Salma M. S. Zeid, Sarah O. Makled","doi":"10.1002/aah.10194","DOIUrl":"10.1002/aah.10194","url":null,"abstract":"<p>\u0000 \u0000 </p>","PeriodicalId":15235,"journal":{"name":"Journal of aquatic animal health","volume":"35 4","pages":"248-262"},"PeriodicalIF":1.2,"publicationDate":"2023-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9886148","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Fangyuan Liu, Jiahao Hou, Fang Yu, Zemao Gu, Ruixue Hu
{"title":"Identification and pathogenicity of multidrug-resistant Elizabethkingia miricola isolated from farmed American bullfrogs Rana catesbeiana in China with in vitro screening of herbal antimicrobial agents","authors":"Fangyuan Liu, Jiahao Hou, Fang Yu, Zemao Gu, Ruixue Hu","doi":"10.1002/aah.10185","DOIUrl":"10.1002/aah.10185","url":null,"abstract":"<p>\u0000 \u0000 </p>","PeriodicalId":15235,"journal":{"name":"Journal of aquatic animal health","volume":"35 2","pages":"117-127"},"PeriodicalIF":1.2,"publicationDate":"2023-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9661527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Lacey R. Hopper, Jolene A. Glenn, Elizabeth MacConnell, James R. Winton, Eveline J. Emmenegger
{"title":"Susceptibility of Pallid Sturgeon to viral hemorrhagic septicemia virus genotype IVb","authors":"Lacey R. Hopper, Jolene A. Glenn, Elizabeth MacConnell, James R. Winton, Eveline J. Emmenegger","doi":"10.1002/aah.10181","DOIUrl":"10.1002/aah.10181","url":null,"abstract":"<p>\u0000 \u0000 </p>","PeriodicalId":15235,"journal":{"name":"Journal of aquatic animal health","volume":"35 2","pages":"88-100"},"PeriodicalIF":1.2,"publicationDate":"2023-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10016355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jessica F. Eraso-Ordoñez, Josué Alonso Yee-Duarte, Nefertiti Taydé Roldán-Wong, Manuel García-Ulloa, Andrés M. Góngora-Gómez, Marcial Arellano-Martínez
{"title":"Histopathological alterations in the gonads of wild white clams Dosinia ponderosa inhabiting a former copper mine locality in the Gulf of California","authors":"Jessica F. Eraso-Ordoñez, Josué Alonso Yee-Duarte, Nefertiti Taydé Roldán-Wong, Manuel García-Ulloa, Andrés M. Góngora-Gómez, Marcial Arellano-Martínez","doi":"10.1002/aah.10184","DOIUrl":"10.1002/aah.10184","url":null,"abstract":"<p>\u0000 \u0000 </p>","PeriodicalId":15235,"journal":{"name":"Journal of aquatic animal health","volume":"35 2","pages":"101-116"},"PeriodicalIF":1.2,"publicationDate":"2023-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10016356","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Vital Heim, R. Dean Grubbs, Matthew J. Smukall, Bryan S. Frazier, John K. Carlson, Tristan L. Guttridge
Objective
Sharks face a high risk of injuries throughout all life stages and are therefore expected to show a good wound closure capacity.
Methods
Here, the wound closure of one major injury and one minor injury to the first dorsal fins of two free-ranging, mature female Great Hammerheads Sphyrna mokarran is described macroscopically.
Result
The sharks showed complete wound closure of single, clean-cut lacerations measuring 24.2 and 11.6 cm in length after an estimated 323 and 138 days. These estimates were based on the observed closure rate and visual confirmation of a complete wound closure upon multiple resightings of the same individuals. Additionally, the posterior lateral displacement of fin-mounted geolocators within the fin and outside of the fin without causing external damage was documented in three additional Great Hammerheads.
Conclusion
These observations supplement findings about wound closure capabilities in elasmobranchs. The documented geolocator displacement furthers the discussion about the safe use of these geolocators to track shark movements but also has implications for future tagging studies.
{"title":"Observations of fin injury closure in Great Hammerheads and implications for the use of fin-mounted geolocators","authors":"Vital Heim, R. Dean Grubbs, Matthew J. Smukall, Bryan S. Frazier, John K. Carlson, Tristan L. Guttridge","doi":"10.1002/aah.10178","DOIUrl":"10.1002/aah.10178","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objective</h3>\u0000 \u0000 <p>Sharks face a high risk of injuries throughout all life stages and are therefore expected to show a good wound closure capacity.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Here, the wound closure of one major injury and one minor injury to the first dorsal fins of two free-ranging, mature female Great Hammerheads <i>Sphyrna mokarran</i> is described macroscopically.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Result</h3>\u0000 \u0000 <p>The sharks showed complete wound closure of single, clean-cut lacerations measuring 24.2 and 11.6 cm in length after an estimated 323 and 138 days. These estimates were based on the observed closure rate and visual confirmation of a complete wound closure upon multiple resightings of the same individuals. Additionally, the posterior lateral displacement of fin-mounted geolocators within the fin and outside of the fin without causing external damage was documented in three additional Great Hammerheads.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>These observations supplement findings about wound closure capabilities in elasmobranchs. The documented geolocator displacement furthers the discussion about the safe use of these geolocators to track shark movements but also has implications for future tagging studies.</p>\u0000 </section>\u0000 </div>","PeriodicalId":15235,"journal":{"name":"Journal of aquatic animal health","volume":"35 2","pages":"53-63"},"PeriodicalIF":1.2,"publicationDate":"2023-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/aah.10178","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9714241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Characterization of two tripartite motif-containing genes from Asian Seabass Lates calcarifer and their expression in response to virus infection and microbial molecular motifs","authors":"Krishnapriya Raji Sathyan, Avinash Premraj, Sajeevan Thavarool Puthiyedathu","doi":"10.1002/aah.10187","DOIUrl":"10.1002/aah.10187","url":null,"abstract":"<p>\u0000 \u0000 </p>","PeriodicalId":15235,"journal":{"name":"Journal of aquatic animal health","volume":"35 3","pages":"169-186"},"PeriodicalIF":1.2,"publicationDate":"2023-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10127177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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