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Journal of Cerebral Blood Flow and Metabolism最新文献

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Early and enduring: Targeting the endothelium for blood-brain barrier protection. 早期和持久:以内皮为目标,保护血脑屏障。
IF 4.9 2区 医学 Q1 ENDOCRINOLOGY & METABOLISM Pub Date : 2024-09-01 Epub Date: 2024-06-21 DOI: 10.1177/0271678X241264086
Silin Wu, Xuefang Sophie Ren, Yejie Shi

The disruption of the blood-brain barrier marks a pivotal early pathological event in ischemic stroke that significantly contributes to subsequent permanent damage. Here we delve into the ramifications of a study conducted by Xu and colleagues, which underscores the essential role of the protein peroxiredoxin-4 in cerebrovascular endothelial cells. Peroxiredoxin-4 was shown to preserve blood-brain barrier integrity during the early stages after cerebral ischemia and reperfusion, ultimately leading to improved long-term outcomes.

血脑屏障的破坏是缺血性中风的一个关键性早期病理事件,对随后的永久性损伤起着重要作用。徐及其同事的研究强调了过氧化物歧化酶-4 蛋白在脑血管内皮细胞中的重要作用。研究表明,过氧化物歧化酶-4 能在脑缺血和再灌注后的早期阶段保护血脑屏障的完整性,最终改善长期预后。
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引用次数: 0
Cpeb4-mediated Dclk2 promotes neuronal pyroptosis induced by chronic cerebral ischemia through phosphorylation of Ehf. Cpeb4介导的Dclk2通过磷酸化Ehf促进慢性脑缺血诱导的神经元热解。
IF 4.9 2区 医学 Q1 ENDOCRINOLOGY & METABOLISM Pub Date : 2024-09-01 Epub Date: 2024-03-21 DOI: 10.1177/0271678X241240590
Miao Sun, Xin Huang, Xuelei Ruan, Xiuli Shang, Mengyang Zhang, Libo Liu, Ping Wang, Ping An, Yang Lin, Jin Yang, Yixue Xue

Chronic cerebral ischemia (CCI) is a clinical syndrome characterised by brain dysfunction due to decreased chronic cerebral perfusion. CCI initiates several inflammatory pathways, including pyroptosis. RNA-binding proteins (RBPs) play important roles in CCI. This study aimed to explore whether the interaction between RBP-Cpeb4 and Dclk2 affected Ehf phosphorylation to regulate neuronal pyroptosis. HT22 cells and mice were used to construct oxygen glucose deprivation (OGD)/CCI models. We found that Cpeb4 and Dclk2 were upregulated in OGD-treated HT22 cells and CCI-induced hippocampal CA1 tissues. Cpeb4 upregulated Dclk2 expression by increasing Dclk2 mRNA stability. Knockdown of Cpeb4 or Dclk2 inhibited neuronal pyroptosis in OGD-treated HT22 cells and CCI-induced hippocampal CA1 tissues. By binding to the promoter regions of Caspase1 and Caspase3, the transcription factor Ehf reduced their promoter activities and inhibited the transcription. Dclk2 phosphorylated Ehf and changed its nucleoplasmic distribution, resulting in the exit of p-Ehf from the nucleus and decreased Ehf levels. It promoted the expression of Caspase1 and Caspase3 and stimulated neuronal pyroptosis of HT22 cells induced by OGD. Cpeb4/Dclk2/Ehf pathway plays an important role in the regulation of cerebral ischemia-induced neuronal pyroptosis.

慢性脑缺血(CCI)是一种临床综合征,其特点是慢性脑灌注减少导致脑功能障碍。慢性脑缺血会引发多种炎症通路,包括脓毒症。RNA结合蛋白(RBPs)在CCI中发挥着重要作用。本研究旨在探讨RBP-Cpeb4和Dclk2之间的相互作用是否会影响Ehf磷酸化,从而调控神经元的热昏迷。研究利用 HT22 细胞和小鼠构建了氧葡萄糖剥夺(OGD)/CCI 模型。我们发现,Cpeb4和Dclk2在OGD处理的HT22细胞和CCI诱导的海马CA1组织中上调。Cpeb4通过增加Dclk2 mRNA的稳定性上调Dclk2的表达。敲除Cpeb4或Dclk2可抑制OGD处理的HT22细胞和CCI诱导的海马CA1组织中神经元的热凋亡。转录因子Ehf通过与Caspase1和Caspase3的启动子区域结合,降低了它们的启动子活性,抑制了它们的转录。Dclk2使Ehf磷酸化,改变了其在核质中的分布,导致p-Ehf从细胞核中退出,Ehf水平下降。它促进了 Caspase1 和 Caspase3 的表达,并刺激了 OGD 诱导的 HT22 细胞的神经元凋亡。Cpeb4/Dclk2/Ehf通路在调控脑缺血诱导的神经元凋亡中发挥了重要作用。
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引用次数: 0
Metabolic coupling between glutamate and N-acetylaspartate in the human brain. 人脑中谷氨酸和 N-乙酰天冬氨酸之间的代谢耦合。
IF 4.9 2区 医学 Q1 ENDOCRINOLOGY & METABOLISM Pub Date : 2024-09-01 Epub Date: 2024-03-14 DOI: 10.1177/0271678X241239783
Sungtak Hong, Jyoti Singh Tomar, Jun Shen

A metabolic coupling between glutamate and N-acetylaspartate measured by in vivo magnetic resonance spectroscopy has been recently reported in the literature with inconsistent findings. In this study, confounders originating from Pearson's spurious correlation of ratios and spectral correlation due to overlapping magnetic resonance spectroscopy signals of glutamate and N-acetylaspartate were practically eliminated to facilitate the determination of any metabolic link between glutamate and N-acetylaspartate in the human brain using in vivo magnetic resonance spectroscopy. In both occipital and medial prefrontal cortices of healthy individuals, correlations between glutamate and N-acetylaspartate were found to be insignificant. Our results do not lend support to a recent hypothesis that N-acetylaspartate serves as a significant reservoir for the rapid replenishment of glutamate during signaling or stress.

最近有文献报道通过体内磁共振波谱测量谷氨酸和 N-乙酰天冬氨酸之间的代谢耦合,但结果并不一致。在这项研究中,为了便于利用体内磁共振波谱测定人脑中谷氨酸和 N-乙酰天冬氨酸之间的代谢联系,研究人员实际上消除了由于谷氨酸和 N-乙酰天冬氨酸的磁共振波谱信号重叠而产生的皮尔逊比率假相关性和光谱相关性所造成的混淆因素。在健康人的枕叶和内侧前额叶皮层中,谷氨酸和 N-乙酰天冬氨酸之间的相关性并不显著。我们的研究结果并不支持最近提出的一种假说,即 N-乙酰天冬氨酸是谷氨酸在信号传导或压力期间快速补充的重要储存库。
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引用次数: 0
Quantification of brain-wide vascular resistivity via ultrafast Doppler in human neonates helps early detection of white matter injury. 通过超快多普勒对人类新生儿全脑血管电阻率进行量化,有助于早期检测白质损伤。
IF 4.9 2区 医学 Q1 ENDOCRINOLOGY & METABOLISM Pub Date : 2024-09-01 Epub Date: 2024-02-10 DOI: 10.1177/0271678X241232197
Flora Faure, Jérôme Baranger, Marianne Alison, Béatrice Boutillier, Alice Frérot, Chung Lim, Grégory Planchette, Mickael Prigent, Mickaël Tanter, Olivier Baud, Valérie Biran, Charlie Demené

Preterm birth is associated with cerebrovascular development disruption and can induce white matter injuries (WMI). Transfontanellar ultrasound Doppler is the most widely used clinical imaging technique to monitor neonatal cerebral vascularisation and haemodynamics based on vascular indexes such as the resistivity index (RI); however, it has poor predictive value for brain damage. Indeed, these RI measurements are currently limited to large vessels, leading to a very limited probing of the brain's vascularisation, which may hinder prognosis. Here we show that ultrafast Doppler imaging (UfD) enables simultaneous quantification, in the whole field of view, of the local RI and vessel diameter, even in small vessels. Combining both pieces of information, we defined two new comprehensive resistivity parameters of the vascular trees. First, we showed that our technique is more sensitive in the early characterisation of the RI modifications between term and preterm neonates and for the first time we could show that the RI depends both on the vessel diameter and vascular territory. We then showed that our parameters can be used for early prediction of WMI. Our results demonstrate the potential of UfD to provide new biomarkers and pave the way for continuous monitoring of neonatal brain resistivity.

早产与脑血管发育障碍有关,并可诱发脑白质损伤(WMI)。根据血管指数(如电阻率指数(RI))监测新生儿脑血管化和血流动力学,经颅骨超声多普勒是最广泛应用的临床成像技术;然而,它对脑损伤的预测价值很低。事实上,这些 RI 测量目前仅限于大血管,导致对脑血管的探测非常有限,这可能会妨碍预后。在这里,我们展示了超快多普勒成像(UfD)能在整个视野中同时量化局部 RI 和血管直径,即使在小血管中也是如此。结合这两项信息,我们定义了血管树的两个新的综合电阻率参数。首先,我们证明了我们的技术在早期鉴定足月和早产新生儿的 RI 变化特征方面更加灵敏,并且首次证明了 RI 同时取决于血管直径和血管区域。随后,我们证明了我们的参数可用于早期预测 WMI。我们的研究结果证明了 UfD 在提供新生物标记物方面的潜力,并为持续监测新生儿脑电阻率铺平了道路。
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引用次数: 0
CO2 cerebrovascular reactivity measured with CBF-MRI in older individuals: Association with cognition, physical function, amyloid and tau proteins. 用 CBF-MRI 测量老年人的 CO2 脑血管反应性:与认知能力、身体功能、淀粉样蛋白和 tau 蛋白的关系。
IF 4.9 2区 医学 Q1 ENDOCRINOLOGY & METABOLISM Pub Date : 2024-09-01 Epub Date: 2024-03-15 DOI: 10.1177/0271678X241240582
Sandeepa Sur, Zixuan Lin, Yang Li, Sevil Yasar, Paul B Rosenberg, Abhay Moghekar, Xirui Hou, Dengrong Jiang, Rita R Kalyani, Kaisha Hazel, George Pottanat, Cuimei Xu, Jay J Pillai, Peiying Liu, Marilyn Albert, Hanzhang Lu

Vascular pathology is the second leading cause of cognitive impairment and represents a major contributing factor in mixed dementia. However, biomarkers for vascular cognitive impairment and dementia (VCID) are under-developed. Here we aimed to investigate the potential role of CO2 Cerebrovascular Reactivity (CVR) measured with phase-contrast quantitative flow MRI in cognitive impairment and dementia. Forty-five (69 ± 7 years) impaired (37 mild-cognitive-impairment and 8 mild-dementia by syndromic diagnosis) and 22 cognitively-healthy-control (HC) participants were recruited and scanned on a 3 T MRI. Biomarkers of AD pathology were measured in cerebrospinal fluid. We found that CBF-CVR was lower (p = 0.027) in the impaired (mean±SE, 3.70 ± 0.15%/mmHg) relative to HC (4.28 ± 0.21%/mmHg). After adjusting for AD pathological markers (Aβ42/40, total tau, and Aβ42/p-tau181), higher CBF-CVR was associated with better cognitive performance, including Montreal Cognitive Assessment, MoCA (p = 0.001), composite cognitive score (p = 0.047), and language (p = 0.004). Higher CBF-CVR was also associated with better physical function, including gait-speed (p = 0.006) and time for five chair-stands (p = 0.049). CBF-CVR was additionally related to the Clinical-Dementia-Rating, CDR, including global CDR (p = 0.026) and CDR Sum-of-Boxes (p = 0.015). CBF-CVR was inversely associated with hemoglobin A1C level (p = 0.017). In summary, CBF-CVR measured with phase-contrast MRI shows associations with cognitive performance, physical function, and disease-severity, independent of AD pathological markers.

血管病变是造成认知障碍的第二大原因,也是混合型痴呆症的主要诱因。然而,血管性认知障碍和痴呆症(VCID)的生物标志物尚不完善。在此,我们旨在研究通过相位对比定量血流磁共振成像测量的二氧化碳脑血管反应性(CVR)在认知障碍和痴呆症中的潜在作用。我们招募了 45 名(69 ± 7 岁)认知功能受损者(37 名轻度认知功能受损者和 8 名经综合征诊断为轻度痴呆者)和 22 名认知功能健康对照者(HC),并对他们进行了 3 T 磁共振成像扫描。对脑脊液中的 AD 病理生物标志物进行了测量。我们发现,相对于 HC(4.28 ± 0.21%/mmHg),受损者的 CBF-CVR 较低(p = 0.027)(平均值±SE,3.70 ± 0.15%/mmHg)。在对注意力缺失病理标记物(Aβ42/40、总 tau 和 Aβ42/p-tau181)进行调整后,较高的 CBF-CVR 与较好的认知表现相关,包括蒙特利尔认知评估(MoCA)(p = 0.001)、综合认知评分(p = 0.047)和语言(p = 0.004)。CBF-CVR 越高,身体功能越好,包括步速(p = 0.006)和五次椅子站立时间(p = 0.049)。此外,CBF-CVR 还与临床痴呆评级(CDR)有关,包括总体 CDR(p = 0.026)和 CDR 框数总和(p = 0.015)。CBF-CVR 与血红蛋白 A1C 水平成反比(p = 0.017)。总之,用相位对比 MRI 测量的 CBF-CVR 显示出与认知能力、身体功能和疾病严重程度的相关性,而与注意力缺失症病理标志物无关。
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引用次数: 0
A dispensable role of mural cell-derived laminin-α5 in intracerebral hemorrhage. 壁细胞衍生的层粘连蛋白-α5在脑出血中发挥着不可或缺的作用。
IF 4.9 2区 医学 Q1 ENDOCRINOLOGY & METABOLISM Pub Date : 2024-09-01 Epub Date: 2024-07-25 DOI: 10.1177/0271678X241264083
Jingsong Ruan, Minkyung Kang, Abhijit Nirwane, Yao Yao

Although most laminin isoforms are neuroprotective in stroke, mural cell-derived laminin-α5 plays a detrimental role in an ischemia-reperfusion model. To determine whether this deleterious effect is an intrinsic feature of mural cell-derived laminin-α5 or unique to ischemic stroke, we performed loss-of-function studies using middle-aged mice with laminin-α5 deficiency in mural cells (α5-PKO) in an intracerebral hemorrhage (ICH) model. Control and α5-PKO mice exhibited comparable changes in all parameters examined, including hematoma size, neuronal death, neurological function, blood-brain barrier integrity, and reactive gliosis. These findings highlight a minimal role of mural cell-derived laminin-α5 in ICH. Together with the detrimental role of mural cell-derived laminin-α5 in ischemic stroke, these negative results in ICH model suggest that mural cell-derived laminin-α5 may exert distinct functions in different diseases.

虽然大多数层粘连蛋白异构体在中风中具有神经保护作用,但壁细胞衍生的层粘连蛋白-α5在缺血再灌注模型中却起着有害作用。为了确定这种有害作用是壁细胞衍生的层粘连蛋白-α5的固有特性还是缺血性脑卒中特有的特性,我们在脑内出血(ICH)模型中使用缺乏壁细胞层粘连蛋白-α5(α5-PKO)的中年小鼠进行了功能缺失研究。对照组小鼠和α5-PKO小鼠在血肿大小、神经元死亡、神经功能、血脑屏障完整性和反应性胶质细胞增多等所有检测指标上都表现出了相似的变化。这些发现突显了壁细胞衍生的层粘连蛋白-α5在 ICH 中的作用微乎其微。加上壁细胞衍生的层粘连蛋白-α5在缺血性中风中的有害作用,这些在ICH模型中的负面结果表明,壁细胞衍生的层粘连蛋白-α5可能在不同的疾病中发挥不同的功能。
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引用次数: 0
A plasma lipid signature in acute human traumatic brain injury: Link with neuronal injury and inflammation markers. 急性人类创伤性脑损伤的血浆脂质特征:与神经元损伤和炎症标志物的联系。
IF 4.9 2区 医学 Q1 ENDOCRINOLOGY & METABOLISM Pub Date : 2024-08-26 DOI: 10.1177/0271678X241276951
Isabell Nessel, Luke Whiley, Simon C Dyall, Adina T Michael-Titus

Traumatic brain injury (TBI) leads to major membrane lipid breakdown. We investigated plasma lipids over 3 days post-TBI, to identify a signature of acute human TBI and assess its correlation with neuronal injury and inflammation. Plasma from patients with TBI (Abbreviated Injury Scale (AIS)3 - serious injury, n = 5; AIS4 - severe injury, n = 8), and controls (n = 13) was analysed for lipidomic profile, neurofilament light (NFL) and cytokines, and the omega-3 index was measured in red blood cells. A lipid signature separated TBI from controls, at 24 and 72 h. Major species driving the separation were: lysophosphatidylcholine (LPC), phosphatidylcholine (PC) and hexosylceramide (HexCer). Docosahexaenoic acid (DHA, 22:6) and LPC (0:0/22:6) decreased post-injury. NFL levels were increased at 24 and 72 h post-injury in AIS4 TBI vs. controls. Interleukin (IL-)6, IL-2 and IL-13 were elevated at 24 h in AIS4 patients vs. controls. NFL and IL-6 were negatively correlated with several lipids. The omega-3 index at admission was low in all patients (controls: 4.3 ± 1.1% and TBI: 4.0 ± 1.1%) and did not change significantly over 3 days post-injury. We have identified specific lipid changes, correlated with markers of injury and inflammation in acute TBI. These observations could inform future lipid-based therapeutic approaches.

创伤性脑损伤(TBI)会导致主要的膜脂分解。我们研究了创伤性脑损伤后 3 天内的血浆脂质,以确定急性人类创伤性脑损伤的特征,并评估其与神经元损伤和炎症的相关性。我们分析了创伤性脑损伤患者(缩写损伤量表(AIS)3 - 重伤,n = 5;AIS4 - 重伤,n = 8)和对照组(n = 13)的血浆脂质体概况、神经丝光(NFL)和细胞因子,并测量了红细胞中的ω-3指数。在 24 小时和 72 小时内,脂质特征将创伤性脑损伤与对照组区分开来。驱动这种区分的主要物种是:溶血磷脂酰胆碱(LPC)、磷脂酰胆碱(PC)和己基甘油酰胺(HexCer)。二十二碳六烯酸(DHA,22:6)和 LPC(0:0/22:6)在损伤后减少。与对照组相比,AIS4创伤性脑损伤患者的 NFL 水平在伤后 24 小时和 72 小时有所增加。与对照组相比,AIS4 患者的白细胞介素 (IL-)6、IL-2 和 IL-13 在伤后 24 小时升高。NFL和IL-6与几种血脂呈负相关。所有患者入院时的ω-3指数都很低(对照组:4.3 ± 1.1%,创伤性脑损伤:4.0 ± 1.1%),并且在伤后3天内没有显著变化。我们发现了与急性创伤性脑损伤的损伤和炎症标志物相关的特定脂质变化。这些观察结果可为未来基于脂质的治疗方法提供参考。
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引用次数: 0
Cerebral blood flow alterations and host genetic association in individuals with long COVID: A transcriptomic-neuroimaging study. 长COVID患者的脑血流改变与宿主基因相关性:转录组学-神经影像学研究。
IF 4.9 2区 医学 Q1 ENDOCRINOLOGY & METABOLISM Pub Date : 2024-08-23 DOI: 10.1177/0271678X241277621
Yao Wang, Ziwei Yang, Xiumei Zheng, Xiao Liang, Lin Wu, Chengsi Wu, Jiankun Dai, Yuan Cao, Meng Li, Fuqing Zhou

Neuroimaging studies have indicated that altered cerebral blood flow (CBF) was associated with the long-term symptoms of postacute sequelae of SARS-CoV-2 infection (PASC), also known as "long COVID". COVID-19 and long COVID were found to be strongly associated with host gene expression. Nevertheless, the relationships between altered CBF, clinical symptoms, and gene expression in the central nervous system (CNS) remain unclear in individuals with long COVID. This study aimed to explore the genetic mechanisms of CBF abnormalities in individuals with long COVID by transcriptomic-neuroimaging spatial association. Lower CBF in the left frontal-temporal gyrus was associated with higher fatigue and worse cognition in individuals with long COVID. This CBF pattern was spatially associated with the expression of 2,178 genes, which were enriched in the molecular functions and biological pathways of COVID-19. Our study suggested that lower CBF is associated with persistent clinical symptoms in long COVID individuals, possibly as a consequence of the complex interactions among multiple COVID-19-related genes, which contributes to our understanding of the impact of adverse CNS outcomes and the trajectory of development to long COVID.

神经影像学研究表明,脑血流(CBF)的改变与 SARS-CoV-2 感染急性期后遗症(PASC)(又称 "长 COVID")的长期症状有关。研究发现,COVID-19 和长 COVID 与宿主基因表达密切相关。然而,长COVID患者的CBF改变、临床症状和中枢神经系统(CNS)基因表达之间的关系仍不清楚。本研究旨在通过转录组学与神经影像学的空间关联,探讨长程COVID患者CBF异常的遗传机制。在长COVID患者中,左侧额颞回较低的CBF与较高的疲劳度和较差的认知能力相关。这种CBF模式与2178个基因的表达存在空间关联,这些基因富集于COVID-19的分子功能和生物通路中。我们的研究表明,较低的CBF与长COVID患者的持续临床症状有关,这可能是多个COVID-19相关基因之间复杂相互作用的结果,有助于我们了解不良中枢神经系统结果的影响以及长COVID的发展轨迹。
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引用次数: 0
Imaging the 5-HT2C receptor with PET: Evaluation of 5-HT2C and 5-HT2A affinity of pimavanserin in the primate brain. 用 PET 对 5-HT2C 受体进行成像:评估灵长类动物大脑中匹马伐林的 5-HT2C 和 5-HT2A 亲和力。
IF 4.9 2区 医学 Q1 ENDOCRINOLOGY & METABOLISM Pub Date : 2024-08-21 DOI: 10.1177/0271678X241276312
Khanum Ridler, Gaia Rizzo, Ethan S Burstein, Anton Forsberg Morén, Vladimir Stepanov, Christer Halldin, Eugenii A Rabiner

Two complimentary techniques were used to estimate occupancy of pimavanserin (a selective 5-HT2A/2C inverse agonist) to 5-HT2A and 5-HT2C receptors in non-human primate brains. One employed the 5-HT2A/2C selective radioligand [11C]CIMBI-36 combined with quantification of binding potentials in brain regions known to be enriched in 5-HT2A (cortex) or 5-HT2C (choroid plexus) receptors to estimate occupancy. Pimavanserin was 6-10 fold more potent displacing [11C]CIMBI-36 from cortex (ED50 = 0.007 mg/kg; EC50 = 0.6 ng/ml) than from choroid plexus (ED50 =0.046 mg/kg; EC50 = 6.0 ng/ml). The assignment of [11C]CIMBI-36 binding to 5-HT2A and 5-HT2C receptors by anatomical brain structure was confirmed using the 5-HT2A selective inverse agonist MDL 100,907 and the 5-HT2C selective antagonist SB 242584 to displace [11C]CIMBI-36. The second technique employed a novel, 5-HT2C selective tracer called [11C]AC1332. [11C]AC1332 bound robustly to choroid plexus, moderately to hippocampus, and minimally to cortex. Pimavanserin displaced [11C]AC1332 with similar potency (ED50 = 0.062 mg/kg; EC50 = 2.5 ng/ml) as its potency displacing [11C]CIMBI-36 binding from choroid plexus. These results demonstrate the feasibility of simultaneously estimating drug occupancy of 5-HT2A and 5-HT2C receptors in vivo, and the utility of a novel 5-HT2C receptor selective tracer ligand.

我们使用了两种互补技术来估算非人灵长类大脑中皮马伐林(一种选择性 5-HT2A/2C 反向激动剂)对 5-HT2A 和 5-HT2C 受体的占有率。其中一种方法采用了 5-HT2A/2C 选择性放射性配体 [11C]CIMBI-36 并结合已知富含 5-HT2A (皮层)或 5-HT2C (脉络丛)受体的脑区结合电位的量化来估计占据率。与脉络丛受体(ED50 = 0.046 mg/kg;EC50 = 6.0 ng/ml)相比,皮质(ED50 = 0.007 mg/kg;EC50 = 0.6 ng/ml)对[11C]CIMBI-36的置换效力高出 6-10 倍。通过使用 5-HT2A 选择性反向激动剂 MDL 100,907 和 5-HT2C 选择性拮抗剂 SB 242584 来置换 [11C]CIMBI-36 与 5-HT2A 和 5-HT2C 受体的结合,确认了[11C]CIMBI-36 与大脑解剖结构的结合。第二种技术采用了一种名为[11C]AC1332 的新型 5-HT2C 选择性示踪剂。[11C]AC1332与脉络丛结合力强,与海马结合力中等,与皮层结合力小。皮马伐林取代[11C]AC1332的效力(ED50 = 0.062 mg/kg;EC50 = 2.5 ng/ml)与其取代脉络丛中[11C]CIMBI-36结合的效力相似。这些结果证明了在体内同时评估药物对 5-HT2A 和 5-HT2C 受体的占用率的可行性,以及新型 5-HT2C 受体选择性示踪配体的实用性。
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引用次数: 0
Oxygen extraction fraction change in M1-M6 brain regions of patients with unilateral or bilateral middle cerebral artery occlusion. 单侧或双侧大脑中动脉闭塞患者脑区 M1-M6 的析氧分数变化。
IF 4.9 2区 医学 Q1 ENDOCRINOLOGY & METABOLISM Pub Date : 2024-08-20 DOI: 10.1177/0271678X241276386
Yu Xiao, Zhenghua Liu, Xinghua Wan

Cerebral blood flow (CBF) and oxygen extraction fraction (OEF) can be measured using arterial spin labeling (ASL) and quantitative susceptibility mapping (QSM) sequences, respectively. ASL and QSM sequences were performed on 13 healthy participants and 46 patients with unilateral or bilateral Middle cerebral artery (MCA) occlusion. M1-M3 and M4-M6 correspond to anterior, lateral, and posterior MCA territories within the insular ribbon and centrum semiovale, respectively. In patients with unilateral MCA occlusion, significant decreases in CBF were observed in the lesions in M1, M3, M5 and M6 regions, as well as in the contralateral M3 and M5 regions. The OEF of the lesion in the M1-M4 and M6 regions, and the contralateral M1-M3 regions were significantly higher. Additionally, the cerebral metabolic rate of oxygen (CMRO2) in the lesions of the M3 and M6 regions, and the contralateral M3 region, were significantly lower compared to the corresponding regions of healthy participants. For patients with bilateral MCA occlusion, the CMRO2 in the left M5 region and the right M3 and M6 regions were significantly lower than that in the corresponding regions of healthy participants. In conclusion, abnormal hemodynamics occur in the contralateral hemisphere of patients with unilateral MCA occlusion.

动脉自旋标记(ASL)和定量易感性映射(QSM)序列可分别测量脑血流(CBF)和氧萃取分数(OEF)。对 13 名健康参与者和 46 名单侧或双侧大脑中动脉(MCA)闭塞患者进行了 ASL 和 QSM 序列分析。M1-M3和M4-M6分别对应岛叶带和半卵圆中心内的MCA前区、外侧区和后区。在单侧 MCA 闭塞的患者中,M1、M3、M5 和 M6 病变区域以及对侧 M3 和 M5 区域的 CBF 均显著下降。M1-M4和M6区域病变的OEF以及对侧M1-M3区域的OEF均明显升高。此外,病变的 M3 和 M6 区域以及对侧 M3 区域的脑氧代谢率(CMRO2)明显低于健康参与者的相应区域。对于双侧 MCA 闭塞患者,左侧 M5 区和右侧 M3 和 M6 区的 CMRO2 明显低于健康参与者相应区域的 CMRO2。总之,单侧 MCA 闭塞患者的对侧半球会出现血液动力学异常。
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引用次数: 0
期刊
Journal of Cerebral Blood Flow and Metabolism
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