Pub Date : 2016-10-01DOI: 10.18869/modares.jcp.5.4.617
C. Moslemkhani, J. Mozafari, M. Shams-bakhsh, E. M. Goltape
One of the best strategies to control bacterial wilt caused by Ralstonia solanacearum (Smith) is generally based on breeding resistant cultivars. The information obtained from the expression of plant defense genes will provide new insight for improving plant resistance against pathogens. This study was to identify inducible genes under defense no death (DND) reaction of tobacco (Nicotiana tabacum)-R. solanacearum interaction using cDNAAFLP technique. In this assay five different primer combinations were used. Out of 1320 Transcript derived fragments (TDF) that were detected, 101 fragments were identified as differentially expressed genes in 0, 24, 48 and 72 hours post inoculation. Most of the differentially expressed genes were obtained 48 hours post inoculation. Following sequencing, most of sequenced TDFs showed homology to known genes interfering in signaling, regulation and defense functions. DND phenotype in tobacco has some similarities specially in signaling process with mechanism associated with induction of the hypersensitive reaction and it is distinct from general defense mechanisms. Keyword: Tobacco, Ralstonia solanacearum, transcriptome, interaction
防治青枯病(Ralstonia solanacearum, Smith)的最佳策略之一是培育抗病品种。从植物防御基因表达中获得的信息将为提高植物抗病能力提供新的思路。本研究旨在鉴定烟草(Nicotiana tabacum)-R抗死亡(defense no death, DND)反应诱导基因。利用cDNAAFLP技术研究龙葵相互作用。在这个实验中，使用了五种不同的引物组合。在检测到的1320个转录本衍生片段(TDF)中，101个片段在接种后0、24、48和72小时被鉴定为差异表达基因。大多数差异表达基因在接种后48小时获得。经测序，大部分tdf序列与已知的信号、调控和防御功能干扰基因具有同源性。烟草DND表型具有一定的相似性，特别是在信号传导过程和诱导过敏反应的相关机制上，与一般的防御机制不同。关键词:烟草，茄枯菌，转录组，互作
{"title":"Transcriptome analysis of tobacco in response to Ralstonia solanacearum infection","authors":"C. Moslemkhani, J. Mozafari, M. Shams-bakhsh, E. M. Goltape","doi":"10.18869/modares.jcp.5.4.617","DOIUrl":"https://doi.org/10.18869/modares.jcp.5.4.617","url":null,"abstract":"One of the best strategies to control bacterial wilt caused by Ralstonia solanacearum (Smith) is generally based on breeding resistant cultivars. The information obtained from the expression of plant defense genes will provide new insight for improving plant resistance against pathogens. This study was to identify inducible genes under defense no death (DND) reaction of tobacco (Nicotiana tabacum)-R. solanacearum interaction using cDNAAFLP technique. In this assay five different primer combinations were used. Out of 1320 Transcript derived fragments (TDF) that were detected, 101 fragments were identified as differentially expressed genes in 0, 24, 48 and 72 hours post inoculation. Most of the differentially expressed genes were obtained 48 hours post inoculation. Following sequencing, most of sequenced TDFs showed homology to known genes interfering in signaling, regulation and defense functions. DND phenotype in tobacco has some similarities specially in signaling process with mechanism associated with induction of the hypersensitive reaction and it is distinct from general defense mechanisms. Keyword: Tobacco, Ralstonia solanacearum, transcriptome, interaction","PeriodicalId":15396,"journal":{"name":"Journal of Crop Protection","volume":"5 1","pages":"617-626"},"PeriodicalIF":0.0,"publicationDate":"2016-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67685664","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2016-10-01DOI: 10.18869/MODARES.JCP.5.4.627
B. Golhasan, R. Heydari, M. Esmaeili, Hadi Ghorbanzad
During a nematological survey, nineteen known species of plantparasitic nematodes belonging to the family Tylenchidae (Tylenchomorpha: Tylenchoidea) were collected and identified from different localities of West Azerbayjan and Kermanshah provinces, Iran. Among them two species, namely Discotylenchus attenuatus and Tylenchus bhitaii, are new records for Iranian nematode fauna, the male of T. bhitaii is recorded for the first time. Also, two previously reported species Filenchus quartus and Tylenchus stachys are illustrated and described. Descriptions, morphometric data, line drawings and microscopic photographs are provided.
{"title":"Description of four species of Tylenchidae Örley, 1880 (Nematoda: Tylenchomorpha) with two new records from Iran","authors":"B. Golhasan, R. Heydari, M. Esmaeili, Hadi Ghorbanzad","doi":"10.18869/MODARES.JCP.5.4.627","DOIUrl":"https://doi.org/10.18869/MODARES.JCP.5.4.627","url":null,"abstract":"During a nematological survey, nineteen known species of plantparasitic nematodes belonging to the family Tylenchidae (Tylenchomorpha: Tylenchoidea) were collected and identified from different localities of West Azerbayjan and Kermanshah provinces, Iran. Among them two species, namely Discotylenchus attenuatus and Tylenchus bhitaii, are new records for Iranian nematode fauna, the male of T. bhitaii is recorded for the first time. Also, two previously reported species Filenchus quartus and Tylenchus stachys are illustrated and described. Descriptions, morphometric data, line drawings and microscopic photographs are provided.","PeriodicalId":15396,"journal":{"name":"Journal of Crop Protection","volume":"5 1","pages":"627-642"},"PeriodicalIF":0.0,"publicationDate":"2016-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67685672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2016-10-01DOI: 10.18869/MODARES.JCP.5.4.603
S. Bahraminejad, Behnaz Seifolahpour, R. Amiri
In this study, anti Alternaria solani effects of essential oils of 11 medicinal and aromatic plant species belonging to four families collected from the west of Iran were investigated based on agar dilution method with five replications at concentration of 1.0 μl/ml. The results showed that the highest inhibitory effect belonged to Oliveria decumbens, Cinnamomum zeylanicum, Carum copticum and Thymus kotschyanus which completely inhibited the mycelial growth of the fungus. Therefore, their activity was examined in lower concentrations, too. The essential oil of O. decumbens at concentration of 0.50 and 0.25 μl/ml completely suppressed the mycelial growth of the fungus. The major constituents of its essential oil were carvacrol (46.53%), thymol (23.75%), p-Cymene (13.70%), γ-terpinene (7.66%) and myristicin (3.65%), respectively. Thus, the essential oil of this species with the highest antiAlternaria activity could be selected for further studies on in vivo application as natural fungicide.
{"title":"Antifungal effects of some medicinal and aromatic plant essential oils against Alternaria solani","authors":"S. Bahraminejad, Behnaz Seifolahpour, R. Amiri","doi":"10.18869/MODARES.JCP.5.4.603","DOIUrl":"https://doi.org/10.18869/MODARES.JCP.5.4.603","url":null,"abstract":"In this study, anti Alternaria solani effects of essential oils of 11 medicinal and aromatic plant species belonging to four families collected from the west of Iran were investigated based on agar dilution method with five replications at concentration of 1.0 μl/ml. The results showed that the highest inhibitory effect belonged to Oliveria decumbens, Cinnamomum zeylanicum, Carum copticum and Thymus kotschyanus which completely inhibited the mycelial growth of the fungus. Therefore, their activity was examined in lower concentrations, too. The essential oil of O. decumbens at concentration of 0.50 and 0.25 μl/ml completely suppressed the mycelial growth of the fungus. The major constituents of its essential oil were carvacrol (46.53%), thymol (23.75%), p-Cymene (13.70%), γ-terpinene (7.66%) and myristicin (3.65%), respectively. Thus, the essential oil of this species with the highest antiAlternaria activity could be selected for further studies on in vivo application as natural fungicide.","PeriodicalId":15396,"journal":{"name":"Journal of Crop Protection","volume":"5 1","pages":"603-616"},"PeriodicalIF":0.0,"publicationDate":"2016-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67685657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2016-09-20DOI: 10.18869/MODARES.JCP.5.3.473
Farnaz Khoshnazar, O. Eini
Beet curly top Iran virus (BCTIV) is a member of the genus Becurtovirus (family Geminiviridae) which constrain host crop production in various geographical regions in Iran. This virus infects several crops such as sugar beet Beta vulgaris and tomato Solanum lycopersicum. BCTIV infected tomato shows leaf curling, leaf distortion and stunting. In this study, we investigated the response of various tomato cultivars to BCTIV infection using an infectious clone of BCTIV under glasshouse condition at the University of Zanjan in 2013-2014. Based on a completely randomized design experiment twelve tomato cultivars were tested for their reaction to BCTIV infection. The replication of virus and symptom production was recorded and analyzed. Based on the obtained coefficient of infection and disease severity index, none of the tested cultivars was found resistance to the virus infection. However, one of the tested cultivars, Super Chief, showed no leaf curling symptom and the virus replicated at a significantly lower level in this cultivar as compared to a susceptible cultivar, Alindi 811, using quantitative PCR. Tomato cultivars including Grosse Lisse and Early Eurbana were grouped as susceptible while the other cultivars including Super Star were moderately susceptible to the virus infection. Therefore, growing this cultivar shows promise for an increase of yields from tomato plants prone to BCTIV infection after complementary field experiments. The screening of more cultivars or wild-type tomatoes for the identification of gene/s providing resistance to this viral disease is suggested.
{"title":"Response of tomato cultivars to agroinfection with Beet curly top Iran virus","authors":"Farnaz Khoshnazar, O. Eini","doi":"10.18869/MODARES.JCP.5.3.473","DOIUrl":"https://doi.org/10.18869/MODARES.JCP.5.3.473","url":null,"abstract":"Beet curly top Iran virus (BCTIV) is a member of the genus Becurtovirus (family Geminiviridae) which constrain host crop production in various geographical regions in Iran. This virus infects several crops such as sugar beet Beta vulgaris and tomato Solanum lycopersicum. BCTIV infected tomato shows leaf curling, leaf distortion and stunting. In this study, we investigated the response of various tomato cultivars to BCTIV infection using an infectious clone of BCTIV under glasshouse condition at the University of Zanjan in 2013-2014. Based on a completely randomized design experiment twelve tomato cultivars were tested for their reaction to BCTIV infection. The replication of virus and symptom production was recorded and analyzed. Based on the obtained coefficient of infection and disease severity index, none of the tested cultivars was found resistance to the virus infection. However, one of the tested cultivars, Super Chief, showed no leaf curling symptom and the virus replicated at a significantly lower level in this cultivar as compared to a susceptible cultivar, Alindi 811, using quantitative PCR. Tomato cultivars including Grosse Lisse and Early Eurbana were grouped as susceptible while the other cultivars including Super Star were moderately susceptible to the virus infection. Therefore, growing this cultivar shows promise for an increase of yields from tomato plants prone to BCTIV infection after complementary field experiments. The screening of more cultivars or wild-type tomatoes for the identification of gene/s providing resistance to this viral disease is suggested.","PeriodicalId":15396,"journal":{"name":"Journal of Crop Protection","volume":"5 1","pages":"473-482"},"PeriodicalIF":0.0,"publicationDate":"2016-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67684897","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2016-09-19DOI: 10.18869/MODARES.JCP.5.3.461
Balaji Panchal, M. S. Kachole
Chilo partellus is a devastating sorghum and maize pest that exists worldwide. C. partellus gut proteases (CPGPs) were isolated, purified and characterized. We analyzed potential trypsin and chymotrypsin inhibitors (TIs) from tuber plants to identify the protease inhibitors (PIs) of CPGPs. PIs from Ipomoea batatas completely inhibited protease activity and growth and led to reduced C. partellus insect larvae weights. One PI was purified from I. batatas tubers and identified as a potent antagonist of CPGPs. In conclusion, we identified Ipomoea batatas inhibitors with significant potential applications in developing resistant transgenic sorghum and maize crops.
{"title":"Inhibition of Chilo partellus (Lepidoptera: Pyralidae) gut proteases with Ipomoea batatas inhibitors","authors":"Balaji Panchal, M. S. Kachole","doi":"10.18869/MODARES.JCP.5.3.461","DOIUrl":"https://doi.org/10.18869/MODARES.JCP.5.3.461","url":null,"abstract":"Chilo partellus is a devastating sorghum and maize pest that exists worldwide. C. partellus gut proteases (CPGPs) were isolated, purified and characterized. We analyzed potential trypsin and chymotrypsin inhibitors (TIs) from tuber plants to identify the protease inhibitors (PIs) of CPGPs. PIs from Ipomoea batatas completely inhibited protease activity and growth and led to reduced C. partellus insect larvae weights. One PI was purified from I. batatas tubers and identified as a potent antagonist of CPGPs. In conclusion, we identified Ipomoea batatas inhibitors with significant potential applications in developing resistant transgenic sorghum and maize crops.","PeriodicalId":15396,"journal":{"name":"Journal of Crop Protection","volume":"5 1","pages":"461-472"},"PeriodicalIF":0.0,"publicationDate":"2016-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67684744","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2016-07-01DOI: 10.18869/MODARES.JCP.5.2.251
M. Mehdipour, Jalal Jalali Sendi, H. Zamanian
Cicada orni Linnaeus, 1758 is an abundant and common cicada across Europe, North Africa, and western Asia including Iran. The male cicada produces a loud calling song for attraction of females from a long distance. The calling songs are known often to be species-specific and to function in part for species recognition. The present study compares variations in calling song of C. orni in Iran with those reported from other areas and also signifies the importance of calling song in species identification. C. orni were collected in Mazandaran, Iran during summer 2010. Four features were used: echeme duration, echeme period, interecheme interval, number of echeme/s and dominant frequency. Findings of present study showed some resemblance in time and frequency domains in the calling song among the populations of C. orni from Iran and those investigated in Greece, Iberian Peninsula and Turkey. The parameters of calling song showed maximal resemblance with species of Kosmas (Greece). The present results do not support C. orni from Iran as an independent subspecies. Thus, it is concluded that every feature of calling song can be effective in species recognition as is the case for several other parameters.
{"title":"Calling song structure of Cicada orni Linnaeus (Hemiptera: Cicadidae) in Iran: A comparative study with other areas","authors":"M. Mehdipour, Jalal Jalali Sendi, H. Zamanian","doi":"10.18869/MODARES.JCP.5.2.251","DOIUrl":"https://doi.org/10.18869/MODARES.JCP.5.2.251","url":null,"abstract":"Cicada orni Linnaeus, 1758 is an abundant and common cicada across Europe, North Africa, and western Asia including Iran. The male cicada produces a loud calling song for attraction of females from a long distance. The calling songs are known often to be species-specific and to function in part for species recognition. The present study compares variations in calling song of C. orni in Iran with those reported from other areas and also signifies the importance of calling song in species identification. C. orni were collected in Mazandaran, Iran during summer 2010. Four features were used: echeme duration, echeme period, interecheme interval, number of echeme/s and dominant frequency. Findings of present study showed some resemblance in time and frequency domains in the calling song among the populations of C. orni from Iran and those investigated in Greece, Iberian Peninsula and Turkey. The parameters of calling song showed maximal resemblance with species of Kosmas (Greece). The present results do not support C. orni from Iran as an independent subspecies. Thus, it is concluded that every feature of calling song can be effective in species recognition as is the case for several other parameters.","PeriodicalId":15396,"journal":{"name":"Journal of Crop Protection","volume":"5 1","pages":"251-257"},"PeriodicalIF":0.0,"publicationDate":"2016-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67683558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2016-07-01DOI: 10.18869/MODARES.JCP.5.2.307
Amir-Reza Piruznia, H. Lotfalizadeh, M. Zargaran
Hemiptarsenus autonomus (Mercet, 1924) (Hymenoptera: Eulophidae, Eulophinae) was found for the first time outside of Europe. Studied specimen was collected by a Malaise trap in the north west of Iran, East-Azarbaijan province, Khajeh (46°38'E & 38°09'N). Current record of Hemiptarsenus species of Iran adds up to seven species. These species and their geographical distribution in Iran are listed.
{"title":"First record of Hemiptarsenus autonomus (Mercet, 1924) (Hymenoptera: Eulophidae) in the Middle East","authors":"Amir-Reza Piruznia, H. Lotfalizadeh, M. Zargaran","doi":"10.18869/MODARES.JCP.5.2.307","DOIUrl":"https://doi.org/10.18869/MODARES.JCP.5.2.307","url":null,"abstract":"Hemiptarsenus autonomus (Mercet, 1924) (Hymenoptera: Eulophidae, Eulophinae) was found for the first time outside of Europe. Studied specimen was collected by a Malaise trap in the north west of Iran, East-Azarbaijan province, Khajeh (46°38'E & 38°09'N). Current record of Hemiptarsenus species of Iran adds up to seven species. These species and their geographical distribution in Iran are listed.","PeriodicalId":15396,"journal":{"name":"Journal of Crop Protection","volume":"5 1","pages":"307-311"},"PeriodicalIF":0.0,"publicationDate":"2016-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67684457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2016-07-01DOI: 10.18869/MODARES.JCP.5.2.169
E. Konani, E. Pourjam, M. Pedram
Leptonchus granulosus, recovered from Lorestan province, is described and illustrated based on morphological, morphometric and molecular data. The Iranian population of the species is characterized by its body length of 1091 - 1374 m, cuticle distinctly two layered, outer layer finely annulated, inner layer distinctly annulated, being partly separated from the body and shriveled after fixation, cap-like lip region separated from the rest of body by constriction, distinctly sclerotised walls of prestoma and stoma, delicate needle-like odontostyle with distinct narrow lumen, 8.0-9.5 m long, slightly arcuate odontophore, 17-21 m long, with arms slightly thickened at base, small pear-shaped pharyngeal bulb, occupying 16.6-24.3% of pharynx length, simple intestine, very long prerectum (617-663 µm long), its junction with intestine having three distinct guard cells located between anterior ovary and cardia, didelphic female reproductive system, composed of equally sized less developed tracts, but with distinct parts (tubular uterus, simple oviduct and ovary), conoid to hemispheroid tail and absence of males. In comparison with the available reports of the species, no remarkable variation in morphometric data ranges was observed. This is the first representative of the genus for Iran's nematode fauna found so far. Molecular phylogenetic studies of Iranian population of L. granulosus using 1669 nt partial sequences of 18S rDNA revealed it forming a clade with another isolate of the species in Bayesian inference (BI) with 0.95 Bayesian posterior probability (BPP).
{"title":"Morphological and molecular characterization of Leptonchus granulosus Cobb, 1920 (Dorylaimida: Leptonchidae) from Iran","authors":"E. Konani, E. Pourjam, M. Pedram","doi":"10.18869/MODARES.JCP.5.2.169","DOIUrl":"https://doi.org/10.18869/MODARES.JCP.5.2.169","url":null,"abstract":"Leptonchus granulosus, recovered from Lorestan province, is described and illustrated based on morphological, morphometric and molecular data. The Iranian population of the species is characterized by its body length of 1091 - 1374 m, cuticle distinctly two layered, outer layer finely annulated, inner layer distinctly annulated, being partly separated from the body and shriveled after fixation, cap-like lip region separated from the rest of body by constriction, distinctly sclerotised walls of prestoma and stoma, delicate needle-like odontostyle with distinct narrow lumen, 8.0-9.5 m long, slightly arcuate odontophore, 17-21 m long, with arms slightly thickened at base, small pear-shaped pharyngeal bulb, occupying 16.6-24.3% of pharynx length, simple intestine, very long prerectum (617-663 µm long), its junction with intestine having three distinct guard cells located between anterior ovary and cardia, didelphic female reproductive system, composed of equally sized less developed tracts, but with distinct parts (tubular uterus, simple oviduct and ovary), conoid to hemispheroid tail and absence of males. In comparison with the available reports of the species, no remarkable variation in morphometric data ranges was observed. This is the first representative of the genus for Iran's nematode fauna found so far. Molecular phylogenetic studies of Iranian population of L. granulosus using 1669 nt partial sequences of 18S rDNA revealed it forming a clade with another isolate of the species in Bayesian inference (BI) with 0.95 Bayesian posterior probability (BPP).","PeriodicalId":15396,"journal":{"name":"Journal of Crop Protection","volume":"5 1","pages":"169-177"},"PeriodicalIF":0.0,"publicationDate":"2016-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67683604","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2016-07-01DOI: 10.18869/MODARES.JCP.5.2.189
Mahmoud Ahmadi Mansourabad, R. Ghaderi, L. Kashi, A. Karegar
Morphological observations are made on several populations of Hoplolaimus indicus and Hoplolaimus seinhorsti, recovered from rhizosphere of mango, tamarind, sour orange and sugarcane from the southern regions of Iran. Detailed studies on the two species Hoplolaimus dubius and H. indicus being separated from each other based on some morphological characters, revealed each of them having intra-specific and overlapping variations in morphology and morphometric ranges, enough for not separating two closely related aforementioned species and as a result, H. dubius is considered as a junior synonym of H. indicus. Observations on H. seinhorsti also supported the Siddiqi's decision on the synonymy of Hoplolaimus sheri with H. seinhorsti. The results of the phylogenetic analyses using D2-D3 expansion segments of 28S rRNA gene were in agreement with the results of previous works, i.e. the classic scheme for assigning species of the genus into two "ancestral" and/or "derived" groups was supported. In phylogenetic trees inferred, using different analysis methods, the Iranian populations of H. indicus were located in the same clade with H. seinhorsti and H. columbus, belonging to "derived" group of species of the genus characterized by having six nuclei in pharyngeal glands, less than four incisures at each lateral field and anteriorly situated position of excretory pore to hemizonid.
{"title":"Observations on Hoplolaimus indicus Sher, 1963 and Hoplolaimus seinhorsti Luc, 1958 (Nematoda: Hoplolaimidae) from Southern Iran","authors":"Mahmoud Ahmadi Mansourabad, R. Ghaderi, L. Kashi, A. Karegar","doi":"10.18869/MODARES.JCP.5.2.189","DOIUrl":"https://doi.org/10.18869/MODARES.JCP.5.2.189","url":null,"abstract":"Morphological observations are made on several populations of Hoplolaimus indicus and Hoplolaimus seinhorsti, recovered from rhizosphere of mango, tamarind, sour orange and sugarcane from the southern regions of Iran. Detailed studies on the two species Hoplolaimus dubius and H. indicus being separated from each other based on some morphological characters, revealed each of them having intra-specific and overlapping variations in morphology and morphometric ranges, enough for not separating two closely related aforementioned species and as a result, H. dubius is considered as a junior synonym of H. indicus. Observations on H. seinhorsti also supported the Siddiqi's decision on the synonymy of Hoplolaimus sheri with H. seinhorsti. The results of the phylogenetic analyses using D2-D3 expansion segments of 28S rRNA gene were in agreement with the results of previous works, i.e. the classic scheme for assigning species of the genus into two \"ancestral\" and/or \"derived\" groups was supported. In phylogenetic trees inferred, using different analysis methods, the Iranian populations of H. indicus were located in the same clade with H. seinhorsti and H. columbus, belonging to \"derived\" group of species of the genus characterized by having six nuclei in pharyngeal glands, less than four incisures at each lateral field and anteriorly situated position of excretory pore to hemizonid.","PeriodicalId":15396,"journal":{"name":"Journal of Crop Protection","volume":"59 1","pages":"189-201"},"PeriodicalIF":0.0,"publicationDate":"2016-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67683707","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2016-07-01DOI: 10.18869/MODARES.JCP.5.2.229
H. Khiavi, A. Davoodi
Powdery mildew, caused by Erysiphe necator is a common and severe fungal disease of grapevine all over the world including Iran. Although the application of chemical fungicides is the most common control method, the use of resistant varieties may be the most effective and environmentally sound strategy for managing the disease. Twenty two grape varieties were evaluated against powdery mildew under natural infection and artificial inoculation during 2011-2013 in Ardabil and Qazvin provinces of Iran. In this study, Perlett, Robee seedless, Flame seedless and Tompson seedless varieties were immune; Mish-Pestan, Khoshnav and Torkamanestan 8 were highly resistant; Torkamanestan 4 and Superior seedless were resistant; Shahani-e-Qazvin and Shast-e-Arous were tolerant; Ghermez-e- Bidaneh, Sefid-e-Bidaneh, Fakhri, Sahebi-e-Ghermez, Peikami, Rishbaba-e- Sefid, Siyah-e-Samarghandi and Rotabi were susceptible and Rishbaba-e- Ghermez, Asgari, and Shahroodi were highly susceptible varieties to grape powdery mildew. In general, findings of this research may be used as resistance genetic sources for management of the disease in national and international programs.
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