Pub Date : 2016-06-28DOI: 10.18869/MODARES.JCP.5.3.447
D. Paul, M. Choudhury
In the present study, seven indigenous, widely distributed plants of Meghalaya, namely, Pinus kesiya Royle (Pinaceae), Lantana camara Linn. (Verbenaceae), Litsea cubeba Lour. (Lauraceae), Gaultheria fragrantissima Wall. (Ericaceae), Mikania micrantha Kunth.(Asteraceae), Ambrosia artemisiifolia Linn.(Asteraceae) and Eupatorium riparium Regel (Asteraceae) were screened for their larvicidal and antifeedant activity against fourth instar larvae of the cotton bollworm, Helicoverpa armigera (Hübner) under laboratory conditions. The crude extracts of all the seven plants demonstrated a dose dependent increase in bioactivity. However the bioactivity of four plants namely, L. camara, G. fragrantissima, L. cubeba and P. kesiya was significantly higher (p ≤ 0.05) than the negative (solvent) control and extracts of A. artemisiifolia, E. riparium and M. micarantha. Methanol extract of L. camara caused highest oral toxicity with larval mortality ranging between 27.77% and 53.33% across the test concentration (0.25%, 0.5% and 1% w/v) while extract of G. fragrantissima demonstrated the highest feeding deterrence with reduction in larval feeding by50.92% and 70.61% at 0.1% and 0.5% respectively. Crude extract of L. cubeba leaves demonstrated high oral toxicity and feeding deterrence while extract of the needles of P. kesiya showed moderate level of oral toxicity as well as feeding deterrence at the highest tested concentration. Phytochemical analysis of the extracts of these four plants, revealed the presence of five different classes of phytocompounds each of which is known to have deleterious effect on insect pests.Thus it may be concluded that four out of the seven plants possess insecticidal property and can be further investigated for the development of a potent botanical insecticide.
{"title":"Larvicidal and antifeedant activity of some indigenous plants of Meghalaya against 4th instar Helicoverpa armigera (Hübner) larvae","authors":"D. Paul, M. Choudhury","doi":"10.18869/MODARES.JCP.5.3.447","DOIUrl":"https://doi.org/10.18869/MODARES.JCP.5.3.447","url":null,"abstract":"In the present study, seven indigenous, widely distributed plants of Meghalaya, namely, Pinus kesiya Royle (Pinaceae), Lantana camara Linn. (Verbenaceae), Litsea cubeba Lour. (Lauraceae), Gaultheria fragrantissima Wall. (Ericaceae), Mikania micrantha Kunth.(Asteraceae), Ambrosia artemisiifolia Linn.(Asteraceae) and Eupatorium riparium Regel (Asteraceae) were screened for their larvicidal and antifeedant activity against fourth instar larvae of the cotton bollworm, Helicoverpa armigera (Hübner) under laboratory conditions. The crude extracts of all the seven plants demonstrated a dose dependent increase in bioactivity. However the bioactivity of four plants namely, L. camara, G. fragrantissima, L. cubeba and P. kesiya was significantly higher (p ≤ 0.05) than the negative (solvent) control and extracts of A. artemisiifolia, E. riparium and M. micarantha. Methanol extract of L. camara caused highest oral toxicity with larval mortality ranging between 27.77% and 53.33% across the test concentration (0.25%, 0.5% and 1% w/v) while extract of G. fragrantissima demonstrated the highest feeding deterrence with reduction in larval feeding by50.92% and 70.61% at 0.1% and 0.5% respectively. Crude extract of L. cubeba leaves demonstrated high oral toxicity and feeding deterrence while extract of the needles of P. kesiya showed moderate level of oral toxicity as well as feeding deterrence at the highest tested concentration. Phytochemical analysis of the extracts of these four plants, revealed the presence of five different classes of phytocompounds each of which is known to have deleterious effect on insect pests.Thus it may be concluded that four out of the seven plants possess insecticidal property and can be further investigated for the development of a potent botanical insecticide.","PeriodicalId":15396,"journal":{"name":"Journal of Crop Protection","volume":"5 1","pages":"447-460"},"PeriodicalIF":0.0,"publicationDate":"2016-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67684651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2016-06-28DOI: 10.18869/MODARES.JCP.5.3.431
M. G. Moghaddam, A. Golizadeh, M. Hassanpour, H. Rafiee-Dastjerdi, J. Razmjou
Lady beetles are the most important predatory species among arthropods, so studying their population parameters gives a clear picture about their life span. The life histories of variegated lady beetle, Hippodamia variegata (Goeze), fed on the English grain aphid, Sitobion avenae Fabricius, on different host plants were studied under laboratory conditions, at 25 ± 1 °C, 65 ± 5% Relative Humidity and a photoperiod of 16:8 (Light: Dark) h. Developmental, survival and fecundity data were analyzed using the age-stage, two-sex life table method. According to the age-stage, two-sex life table, the net reproductive rates (R0) of ladybird fed on S. avenae reared on Triticum aestivum var. tajan, Hordeum vulgare var. Dasht, Zea mays var. single cross 704 and Sorghum durra var. Speed feed were estimated to be 235.38 ± 36.15, 190.74 ± 33.37, 293.54 ± 46.66 and 137.46 ± 23.66 female offspring / female, respectively. Based on the results, the intrinsic rate of increase as an index of population increase of H. variegata, was 0.181 ± 0.006, 0.179 ± 0.006, 0.163 ± 0.006 and 0.162 ± 0.006 h 1 preyed on host reared on T. aestivum var. tajan Z. mays var. single cross 704 S. durra var. Speed feed and H. vulgare var. Dasht, respectively. Our findings demonstrate that by using the age-stage, two-sex life table we can accurately describe the growth, survival and development of the predator. Our finding may provide basic information for developing aphid biological control programs.
瓢虫是节肢动物中最重要的掠食性物种，因此研究瓢虫的种群参数可以清楚地了解瓢虫的寿命。在25±1℃、65±5%相对湿度、16:8(光:暗)h光照条件下，研究了以英国粒蚜为食的斑瓢虫(Hippodamia variegata, Goeze)在不同寄主植物上的生活史，并采用年龄分期、两性寿命表法分析了斑瓢虫的发育、存活和繁殖力数据。根据龄期、两性生命表，采食以小麦(Triticum aestivum var. tajan)、普通小麦(Hordeum vulgare var. Dasht)、玉米(Zea maays var. 704)单交和高粱(Sorghum durra var. Speed)为食的瓢虫净繁殖率(R0)分别为235.38±36.15、190.74±33.37、293.54±46.66和137.46±23.66个雌虫/雌虫。结果表明，以tajan Z. mays var、704 S. durra var、Speed饲料和vulgare var. Dasht饲养的寄主为食，变异小蠊种群的内在增长率分别为0.181±0.006、0.179±0.006、0.163±0.006和0.162±0.006 h。我们的研究结果表明，通过使用年龄阶段，两性生命表，我们可以准确地描述捕食者的生长，生存和发展。本研究结果可为蚜虫生物防治提供依据。
{"title":"Demographic traits of Hippodamia variegata (Goeze) (Coleoptera: Coccinellidae) fed on Sitobion avenae Fabricius (Hemiptera: Aphididae)","authors":"M. G. Moghaddam, A. Golizadeh, M. Hassanpour, H. Rafiee-Dastjerdi, J. Razmjou","doi":"10.18869/MODARES.JCP.5.3.431","DOIUrl":"https://doi.org/10.18869/MODARES.JCP.5.3.431","url":null,"abstract":"Lady beetles are the most important predatory species among arthropods, so studying their population parameters gives a clear picture about their life span. The life histories of variegated lady beetle, Hippodamia variegata (Goeze), fed on the English grain aphid, Sitobion avenae Fabricius, on different host plants were studied under laboratory conditions, at 25 ± 1 °C, 65 ± 5% Relative Humidity and a photoperiod of 16:8 (Light: Dark) h. Developmental, survival and fecundity data were analyzed using the age-stage, two-sex life table method. According to the age-stage, two-sex life table, the net reproductive rates (R0) of ladybird fed on S. avenae reared on Triticum aestivum var. tajan, Hordeum vulgare var. Dasht, Zea mays var. single cross 704 and Sorghum durra var. Speed feed were estimated to be 235.38 ± 36.15, 190.74 ± 33.37, 293.54 ± 46.66 and 137.46 ± 23.66 female offspring / female, respectively. Based on the results, the intrinsic rate of increase as an index of population increase of H. variegata, was 0.181 ± 0.006, 0.179 ± 0.006, 0.163 ± 0.006 and 0.162 ± 0.006 h 1 preyed on host reared on T. aestivum var. tajan Z. mays var. single cross 704 S. durra var. Speed feed and H. vulgare var. Dasht, respectively. Our findings demonstrate that by using the age-stage, two-sex life table we can accurately describe the growth, survival and development of the predator. Our finding may provide basic information for developing aphid biological control programs.","PeriodicalId":15396,"journal":{"name":"Journal of Crop Protection","volume":"5 1","pages":"431-445"},"PeriodicalIF":0.0,"publicationDate":"2016-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67684575","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2016-06-26DOI: 10.18869/MODARES.JCP.5.3.413
F. Yarahmadi, Z. Salehi, H. Lotfalizadeh
This is the first report of two ectoparasitoid wasps, Elachertus inunctus (Nees, 1834) in Iran and Elachertus pulcher (Erdös, 1961) (Hym.: Eulophidae) in the world, that parasitize larvae of the tomato leaf miner, Tuta absoluta (Meyrick, 1917) (Lep.: Gelechiidae). The specimens were collected from tomato fields and greenhouses in Ahwaz, Khouzestan province (south west of Iran). Both species are new records for fauna of Iran. The knowledge about these parasitoids is still scanty. The potential of these parasitoids for biological control of T. absoluta in tomato fields and greenhouses should be investigated.
{"title":"Two species of the genus Elachertus Spinola (Hym.: Eulophidae) new larval ectoparasitoids of Tuta absoluta (Meyreck) (Lep.: Gelechidae)","authors":"F. Yarahmadi, Z. Salehi, H. Lotfalizadeh","doi":"10.18869/MODARES.JCP.5.3.413","DOIUrl":"https://doi.org/10.18869/MODARES.JCP.5.3.413","url":null,"abstract":"This is the first report of two ectoparasitoid wasps, Elachertus inunctus (Nees, 1834) in Iran and Elachertus pulcher (Erdös, 1961) (Hym.: Eulophidae) in the world, that parasitize larvae of the tomato leaf miner, Tuta absoluta (Meyrick, 1917) (Lep.: Gelechiidae). The specimens were collected from tomato fields and greenhouses in Ahwaz, Khouzestan province (south west of Iran). Both species are new records for fauna of Iran. The knowledge about these parasitoids is still scanty. The potential of these parasitoids for biological control of T. absoluta in tomato fields and greenhouses should be investigated.","PeriodicalId":15396,"journal":{"name":"Journal of Crop Protection","volume":"5 1","pages":"413-418"},"PeriodicalIF":0.0,"publicationDate":"2016-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67684990","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2016-06-26DOI: 10.18869/modares.jcp.5.3.419
Helen Mohammadi, A. Saboori, A. Z. Golpayegani
While searching for food, predators use herbivore induced plant volatiles (HIPV), host plant volatiles and those related to con/heterospecifics to find their prey. Not only the volatile components vary among plant species, but also the predator perception of these components might differ among species and samples. Here, we compared the olfactory response of two samples (Turkey and University of Tehran) of Phytoseiulus persimilis Athias-Henriot (Acari: Phytoseiidae) when received herbivore induced plant volatiles (HIPV) from Tetranychus urticae Koch (Acari: Tetranychidae) infested cucumber and bean leaves, along with testing the effect of rearing experience of Turkey sample on its olfactory response in our laboratory conditions. Our data showed that P. persimilis of both samples significantly moved towards leaf odors (either cucumber or bean) when they received clean air from the alternative arm. For both samples, the predator did not make a preference between clean bean and cucumber leaves. When the predators were offered a choice between T. urticae infested bean and cucumber leaves, they significantly moved towards bean leaves in both samples. Rearing experience did not affect the predator choice of host plant species and P. persimilis from both samples preferred odors related to clean leaves rather than clean air. The number of experienced predatory mites moved towards T. urticae infested bean leaves was significantly higher than that preferred T. urticae infested cucumber leaves in both samples. We discussed whether their similar olfactory responses would be related to their experience of previous rearing conditions.
{"title":"The olfactory response of Phytoseiulus persimilis on Tetranychus urticae infested bean and cucumber leaves","authors":"Helen Mohammadi, A. Saboori, A. Z. Golpayegani","doi":"10.18869/modares.jcp.5.3.419","DOIUrl":"https://doi.org/10.18869/modares.jcp.5.3.419","url":null,"abstract":"While searching for food, predators use herbivore induced plant volatiles (HIPV), host plant volatiles and those related to con/heterospecifics to find their prey. Not only the volatile components vary among plant species, but also the predator perception of these components might differ among species and samples. Here, we compared the olfactory response of two samples (Turkey and University of Tehran) of Phytoseiulus persimilis Athias-Henriot (Acari: Phytoseiidae) when received herbivore induced plant volatiles (HIPV) from Tetranychus urticae Koch (Acari: Tetranychidae) infested cucumber and bean leaves, along with testing the effect of rearing experience of Turkey sample on its olfactory response in our laboratory conditions. Our data showed that P. persimilis of both samples significantly moved towards leaf odors (either cucumber or bean) when they received clean air from the alternative arm. For both samples, the predator did not make a preference between clean bean and cucumber leaves. When the predators were offered a choice between T. urticae infested bean and cucumber leaves, they significantly moved towards bean leaves in both samples. Rearing experience did not affect the predator choice of host plant species and P. persimilis from both samples preferred odors related to clean leaves rather than clean air. The number of experienced predatory mites moved towards T. urticae infested bean leaves was significantly higher than that preferred T. urticae infested cucumber leaves in both samples. We discussed whether their similar olfactory responses would be related to their experience of previous rearing conditions.","PeriodicalId":15396,"journal":{"name":"Journal of Crop Protection","volume":"5 1","pages":"419-430"},"PeriodicalIF":0.0,"publicationDate":"2016-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67684513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2016-06-22DOI: 10.18869/modares.jcp.5.3.397
S. Shahbazi, H. Askari, S. Mojerlou
This study was undertaken to find out the optimum physicochemical parameters of fermentation, i.e. pH, incubation temperature and incubation time for the cellulase enzyme production of Trichoderma harzianum. The extracellular protein content was estimated by the dye binding method of Bradford. Endo-glucanase (EG), exoglucanase (or Cellobiohydrolase; CBH), β-glucosidase and total cellulase activity were investigated. The molecular weight of cellulase enzymes was studied using SDS-PAGE. To identify the predominant catalytic components in optimum conditions of enzyme production, cellulases were separated by an adapted two-dimensional electrophoresis technique. Estimated optimum conditions for cellulase enzyme were found as: pH 6.5, incubation temperature 28°C and incubation time 72 h. The SDS-PAGE profiles showed several enzyme bonds such as CBHs, EGs and BGLs. The T. harzianum had both enzyme bonds of Cel7A (CBHI) and Cel7B (EG). Finally, the results of the 2D PAGE analysis showed that the profile of protein in optimium conditions of enzyme production had several enzymes such as CBHs, EGs and the high values of cellulose activity due to synergism that occurred between the CBH and EG.
{"title":"The impact of different physicochemical parameters of fermentation on extracellular cellulolytic enzyme production by Trichoderma harzianum","authors":"S. Shahbazi, H. Askari, S. Mojerlou","doi":"10.18869/modares.jcp.5.3.397","DOIUrl":"https://doi.org/10.18869/modares.jcp.5.3.397","url":null,"abstract":"This study was undertaken to find out the optimum physicochemical parameters of fermentation, i.e. pH, incubation temperature and incubation time for the cellulase enzyme production of Trichoderma harzianum. The extracellular protein content was estimated by the dye binding method of Bradford. Endo-glucanase (EG), exoglucanase (or Cellobiohydrolase; CBH), β-glucosidase and total cellulase activity were investigated. The molecular weight of cellulase enzymes was studied using SDS-PAGE. To identify the predominant catalytic components in optimum conditions of enzyme production, cellulases were separated by an adapted two-dimensional electrophoresis technique. Estimated optimum conditions for cellulase enzyme were found as: pH 6.5, incubation temperature 28°C and incubation time 72 h. The SDS-PAGE profiles showed several enzyme bonds such as CBHs, EGs and BGLs. The T. harzianum had both enzyme bonds of Cel7A (CBHI) and Cel7B (EG). Finally, the results of the 2D PAGE analysis showed that the profile of protein in optimium conditions of enzyme production had several enzymes such as CBHs, EGs and the high values of cellulose activity due to synergism that occurred between the CBH and EG.","PeriodicalId":15396,"journal":{"name":"Journal of Crop Protection","volume":"57 1","pages":"397-412"},"PeriodicalIF":0.0,"publicationDate":"2016-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67684900","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2016-06-21DOI: 10.18869/MODARES.JCP.5.3.389
M. Eslahi, S. Mojerlou
Stripe rust cause by Puccinia striiformis f. sp. tritici is one of the most important diseases of wheat and can cause severe yield loss in many wheat growing regions of the world including Iran. To determine yield loss caused by this disease and evaluate the effect of some chemical components on reduction of yield loss in south of Iran, field experiments were carried out in split plot design with three replications at Ahvaz research station during 2014-2015. Three cultivars; Chamran, Virinak and Boolani, were used and artificial inoculation was performed using an isolate which was collected from south of Iran and designated as Yr27 race variant. Meanwhile the effects of propiconazole and some herbicides on yield loss reduction were studied. In this study, grain yield and area under disease progress curve (AUDPC) were measured. Statistical analysis showed that the level of the yield reduction was significantly different in the three studied cultivars and different treatments. Propiconazole could control the disease significantly. The highest yield loss was observed for cv. Boolani in both with (9%) and without (54%) fungicide treatments. Combined application of propiconazole and herbicides significantly reduced yield loss compared with using them separately. The results of crop loss modeling using integral and multiple point regression models showed that the integral model (L = 0.017AUDPC-17.831) could explain more than 69% of AUDPC variations in relation to crop loss in all cultivars. In multiple point models, disease severity at various dates was considered as independent variable and crop loss percentage as dependent variable. This model with the highest coefficient of determination had the best fitness for crop loss estimation. The results showed that the disease severity at GS39, GS45, GS50 and GS60 stages (Zadok's scale) were more important for crop loss prediction than those in other phenological stages.
{"title":"Modeling of crop loss caused by Puccinia striiformis f. sp. tritici in three common wheat cultivars in southern Iran","authors":"M. Eslahi, S. Mojerlou","doi":"10.18869/MODARES.JCP.5.3.389","DOIUrl":"https://doi.org/10.18869/MODARES.JCP.5.3.389","url":null,"abstract":"Stripe rust cause by Puccinia striiformis f. sp. tritici is one of the most important diseases of wheat and can cause severe yield loss in many wheat growing regions of the world including Iran. To determine yield loss caused by this disease and evaluate the effect of some chemical components on reduction of yield loss in south of Iran, field experiments were carried out in split plot design with three replications at Ahvaz research station during 2014-2015. Three cultivars; Chamran, Virinak and Boolani, were used and artificial inoculation was performed using an isolate which was collected from south of Iran and designated as Yr27 race variant. Meanwhile the effects of propiconazole and some herbicides on yield loss reduction were studied. In this study, grain yield and area under disease progress curve (AUDPC) were measured. Statistical analysis showed that the level of the yield reduction was significantly different in the three studied cultivars and different treatments. Propiconazole could control the disease significantly. The highest yield loss was observed for cv. Boolani in both with (9%) and without (54%) fungicide treatments. Combined application of propiconazole and herbicides significantly reduced yield loss compared with using them separately. The results of crop loss modeling using integral and multiple point regression models showed that the integral model (L = 0.017AUDPC-17.831) could explain more than 69% of AUDPC variations in relation to crop loss in all cultivars. In multiple point models, disease severity at various dates was considered as independent variable and crop loss percentage as dependent variable. This model with the highest coefficient of determination had the best fitness for crop loss estimation. The results showed that the disease severity at GS39, GS45, GS50 and GS60 stages (Zadok's scale) were more important for crop loss prediction than those in other phenological stages.","PeriodicalId":15396,"journal":{"name":"Journal of Crop Protection","volume":"5 1","pages":"389-395"},"PeriodicalIF":0.0,"publicationDate":"2016-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67684717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2016-06-15DOI: 10.18869/MODARES.JCP.5.3.377
S. Mohammadi, J. Soltani, K. Piri
The fungal genus Paecilomyces comprises numerous pathogenic and saprobic species, which are regularly isolated from insects, nematodes, soil, air, food, paper and many other materials. Some of the Paecilomyces species have been known to exhibit capabilities for curing human diseases. Here, bioactivities of metabolites from some soil inhabitant and invertebrate pathogenic Paecilomyces species were explored against a panel of target prokaryotic and eukaryotic microorganisms. First, Petri plate assays indicated that all tested Paecilomyces species were capable of producing diffusible metabolites and volatile compounds with antifungal activities against Pyricularia oryzae and Saccharomyces cerevisiae. Subsequently, the metabolites of the Paecilomyces species were extracted and the growth inhibitory and antimitotic effects of extra-cellular metabolites were shown using the yeast S. cerevisiae as a model. Further research indicated some antibacterial activity of extra-cellular metabolites from Paecilomyces species against human pathogenic bacteria Staphylococcus aureus, Bacillus subtilis, Streptococcus pyogenes (G) and Escherichia coli, Pseudomonas aeruginosa and Salmonella typhi (G). These findings indicate that the Paecilomyces species, either saprobic or pathogenic, have a strong arsenal of bioactive metabolites which show inhibitory or cytotoxic effects against other microorganisms, with a potential for application in agroforestry and medicine.
{"title":"Soilborne and invertebrate pathogenic Paecilomyces species show activity against pathogenic fungi and bacteria","authors":"S. Mohammadi, J. Soltani, K. Piri","doi":"10.18869/MODARES.JCP.5.3.377","DOIUrl":"https://doi.org/10.18869/MODARES.JCP.5.3.377","url":null,"abstract":"The fungal genus Paecilomyces comprises numerous pathogenic and saprobic species, which are regularly isolated from insects, nematodes, soil, air, food, paper and many other materials. Some of the Paecilomyces species have been known to exhibit capabilities for curing human diseases. Here, bioactivities of metabolites from some soil inhabitant and invertebrate pathogenic Paecilomyces species were explored against a panel of target prokaryotic and eukaryotic microorganisms. First, Petri plate assays indicated that all tested Paecilomyces species were capable of producing diffusible metabolites and volatile compounds with antifungal activities against Pyricularia oryzae and Saccharomyces cerevisiae. Subsequently, the metabolites of the Paecilomyces species were extracted and the growth inhibitory and antimitotic effects of extra-cellular metabolites were shown using the yeast S. cerevisiae as a model. Further research indicated some antibacterial activity of extra-cellular metabolites from Paecilomyces species against human pathogenic bacteria Staphylococcus aureus, Bacillus subtilis, Streptococcus pyogenes (G) and Escherichia coli, Pseudomonas aeruginosa and Salmonella typhi (G). These findings indicate that the Paecilomyces species, either saprobic or pathogenic, have a strong arsenal of bioactive metabolites which show inhibitory or cytotoxic effects against other microorganisms, with a potential for application in agroforestry and medicine.","PeriodicalId":15396,"journal":{"name":"Journal of Crop Protection","volume":"5 1","pages":"377-387"},"PeriodicalIF":0.0,"publicationDate":"2016-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67684118","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2016-06-14DOI: 10.18869/MODARES.JCP.5.3.369
R. Bahrami, F. Kocheili, M. Ziaee
In this study, the efficiency of essential oils from asafoetida, Ferula assa foetida L., geranium, Pelargonium hortorum L.H. Bailey and walnut leaves, Juglans regia F. was investigated on nutritional indices of Rhyzopertha dominica (F.) adults. Wheat grains were treated with different concentrations of the oils. The nutritional indices: relative growth rate (RGR), relative consumption rate (RCR), efficiency of conversion of ingested food (ECI) and feeding deterrence index (FDI) were measured at 28 ± 2 °C, 65 ± 5% R. H. in continuous darkness. RGR, RCR and ECI deceased as the concentration level was increased. While, FDI percent increased significantly as the concentration level increased. Asafoetida oil had the most efficiency on nutritional indices and 28.61 ppm of the oil was enough to decrease RGR, RCR and ECI% to 0.032 mg/mg/day, 0.444 mg/mg/day and 6.994%, respectively. FDI percent of adults exposed to 6.5 ppm of asafoetida oil was 13.31% which increased upto 64.62% at the concentration of 28.61 ppm. In the second experiment, the effect of tested essential oils was evaluated on F1 progeny reduction of R. dominica. Walnut and asafoetida leaves essential oils significantly suppressed progeny production by (59.92%) and (53.2%) respectively.
{"title":"Effects of asafoetida, geranium and walnut leaves essential oils on nutritional indices and progeny reduction on Rhyzopertha dominica adults (Coleoptera: Bostrychidae)","authors":"R. Bahrami, F. Kocheili, M. Ziaee","doi":"10.18869/MODARES.JCP.5.3.369","DOIUrl":"https://doi.org/10.18869/MODARES.JCP.5.3.369","url":null,"abstract":"In this study, the efficiency of essential oils from asafoetida, Ferula assa foetida L., geranium, Pelargonium hortorum L.H. Bailey and walnut leaves, Juglans regia F. was investigated on nutritional indices of Rhyzopertha dominica (F.) adults. Wheat grains were treated with different concentrations of the oils. The nutritional indices: relative growth rate (RGR), relative consumption rate (RCR), efficiency of conversion of ingested food (ECI) and feeding deterrence index (FDI) were measured at 28 ± 2 °C, 65 ± 5% R. H. in continuous darkness. RGR, RCR and ECI deceased as the concentration level was increased. While, FDI percent increased significantly as the concentration level increased. Asafoetida oil had the most efficiency on nutritional indices and 28.61 ppm of the oil was enough to decrease RGR, RCR and ECI% to 0.032 mg/mg/day, 0.444 mg/mg/day and 6.994%, respectively. FDI percent of adults exposed to 6.5 ppm of asafoetida oil was 13.31% which increased upto 64.62% at the concentration of 28.61 ppm. In the second experiment, the effect of tested essential oils was evaluated on F1 progeny reduction of R. dominica. Walnut and asafoetida leaves essential oils significantly suppressed progeny production by (59.92%) and (53.2%) respectively.","PeriodicalId":15396,"journal":{"name":"Journal of Crop Protection","volume":"5 1","pages":"369-375"},"PeriodicalIF":0.0,"publicationDate":"2016-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67684498","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2016-06-01DOI: 10.18869/MODARES.JCP.5.3.349
Tahmineh Naeem-Abadi, M. Keshavarzi
Blue mold disease caused by Penicillium expansum is a major post- harvest disease of apples. In this research, the biochemical basis of apple resistance to this pathogen was studied in two relatively resistant and susceptible cultivars, Granny smith and Mashhad, respectively. The activities of catalase (CAT), peroxidase (POX), superoxide dismutase (SOD) and polyphenol oxidase (PPO) enzymes and polyphenol content were compared at different time intervals of 0 to 7 days. Based on the results, fruit polyphenol content of Granny smith was higher than that of Mashhad PPO, SOD and CAT activity was higher in Granny smith than Mashhad but CAT activity decreased three days post-treatment. No detectable difference was found in POX activities in the two cultivars. It is concluded that polyphenols contribute in apple resistance to blue mold. Activation of PPO and SOD, lack of POX activity and decrease of CAT activity, all together, could lead to a toxic environment around the blue mold fungus.
{"title":"Involvement of protective enzymes and phenols in decay (Penicillium expansum) resistance in apple","authors":"Tahmineh Naeem-Abadi, M. Keshavarzi","doi":"10.18869/MODARES.JCP.5.3.349","DOIUrl":"https://doi.org/10.18869/MODARES.JCP.5.3.349","url":null,"abstract":"Blue mold disease caused by Penicillium expansum is a major post- harvest disease of apples. In this research, the biochemical basis of apple resistance to this pathogen was studied in two relatively resistant and susceptible cultivars, Granny smith and Mashhad, respectively. The activities of catalase (CAT), peroxidase (POX), superoxide dismutase (SOD) and polyphenol oxidase (PPO) enzymes and polyphenol content were compared at different time intervals of 0 to 7 days. Based on the results, fruit polyphenol content of Granny smith was higher than that of Mashhad PPO, SOD and CAT activity was higher in Granny smith than Mashhad but CAT activity decreased three days post-treatment. No detectable difference was found in POX activities in the two cultivars. It is concluded that polyphenols contribute in apple resistance to blue mold. Activation of PPO and SOD, lack of POX activity and decrease of CAT activity, all together, could lead to a toxic environment around the blue mold fungus.","PeriodicalId":15396,"journal":{"name":"Journal of Crop Protection","volume":"5 1","pages":"349-357"},"PeriodicalIF":0.0,"publicationDate":"2016-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67684368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2016-06-01DOI: 10.18869/MODARES.JCP.5.3.343
A. Hemmati, B. Mansoori
Field experiment was conducted in 2010 and 2011, in order to study the effects of NPK fertilizers on the incidence of Verticillium wilt of a commercial variety 'Agria' in deficient soil at Eghlid Agriculture Research Station in the north of Fars province, Iran. Experimental design was randomized complete blocks and each treatment replicated three times. Treatments were the amounts of recommended NPK rate (180N, 125P, 100K), 20% and 40 % less and more than the rate based on the soil tests. Soil at planting sites was inoculated with 5 ml of microsclerotia suspension (70 ms/ml). Tubers weight, plant height and disease scores were recorded. Two-year combined analysis indicated that there were significant differences at 1% level between the treatments. The results showed a marked decrease in Verticillium wilt incidence and increase in yield when NPK was used at the recommended rate. Lowest disease score and highest yield even more than the cultivar potential were obtained in the plots when N was used 40 % more than the recommended level of the soil test (252 N), combined with K at the level of the soil test (100 K). P was essential for plant growth but had no effect on the disease.
2010年和2011年，在伊朗法尔斯省北部的Eghlid农业研究站，为研究氮磷钾肥料对商业品种“Agria”在亏缺土壤中黄萎病发生的影响，进行了田间试验。实验设计为随机完整区，每个处理重复3次。处理分别为:在土壤试验基础上，按推荐氮磷钾用量(180N、125P、100K)、比推荐氮磷钾用量少20%、多40%。用微菌核菌悬浮液5 ml (70 ms/ml)接种种植场地土壤。记录块茎重量、株高和病害评分。两年联合分析表明，两种治疗之间存在1%水平的显著差异。结果表明，按推荐用量施用氮磷钾可显著降低黄萎病发病率，提高产量。施氮量高于土壤试验推荐水平(252 N) 40%，施钾量达到土壤试验水平(100 K)时，土壤病害评分最低，产量最高，甚至超过品种潜力。磷对植株生长是必需的，但对病害没有影响。
{"title":"Sufficient application of NPK fertilizers: A practical and efficient strategy in the management of Verticillium wilt of potato var. Agria","authors":"A. Hemmati, B. Mansoori","doi":"10.18869/MODARES.JCP.5.3.343","DOIUrl":"https://doi.org/10.18869/MODARES.JCP.5.3.343","url":null,"abstract":"Field experiment was conducted in 2010 and 2011, in order to study the effects of NPK fertilizers on the incidence of Verticillium wilt of a commercial variety 'Agria' in deficient soil at Eghlid Agriculture Research Station in the north of Fars province, Iran. Experimental design was randomized complete blocks and each treatment replicated three times. Treatments were the amounts of recommended NPK rate (180N, 125P, 100K), 20% and 40 % less and more than the rate based on the soil tests. Soil at planting sites was inoculated with 5 ml of microsclerotia suspension (70 ms/ml). Tubers weight, plant height and disease scores were recorded. Two-year combined analysis indicated that there were significant differences at 1% level between the treatments. The results showed a marked decrease in Verticillium wilt incidence and increase in yield when NPK was used at the recommended rate. Lowest disease score and highest yield even more than the cultivar potential were obtained in the plots when N was used 40 % more than the recommended level of the soil test (252 N), combined with K at the level of the soil test (100 K). P was essential for plant growth but had no effect on the disease.","PeriodicalId":15396,"journal":{"name":"Journal of Crop Protection","volume":"52 1","pages":"343-348"},"PeriodicalIF":0.0,"publicationDate":"2016-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67684263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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