Journal of Clinical Laboratory Analysis最新文献

{"title":"Integrated Transcriptomic and Metabolomic Analysis Revealed That Tryptophan Metabolism–Related Metabolites Are Biomarkers of Trastuzumab Resistance","authors":"Xinxin Si,&nbsp;Hanzhe Wu,&nbsp;Yiwei Cao,&nbsp;Bangbang Wei,&nbsp;Juan Wei,&nbsp;Zhongcheng Wang,&nbsp;Changfei Mao","doi":"10.1002/jcla.70124","DOIUrl":"10.1002/jcla.70124","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Trastuzumab resistance strongly affects the prognosis of HER2-positive breast cancer patients, and tryptophan metabolism plays a key role in cancer progression and cancer prognosis. However, the relationship between trastuzumab resistance and tryptophan metabolism in HER2-positive breast cancer remains unclear.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Four HER2-positive breast cancer cell lines (TS: BT-474, SK-BR-3; TR: HCC1954, JIMT-1) were cultured in specific media. Metabolites from cells, tissues, and serum were extracted via pretreatment, centrifugation, drying, and redissolution. UPLC-MS/MS was used for metabolite detection, while tryptophan metabolism-related gene expression was measured using qRT-PCR. <i>T</i>-test was used to calculate statistical differences. Data visualization, machine learning models construction (RF, LASSO logistic regression), and nomogram were achieved through R language.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>PLS-DA showed distinct separation between TS and TR breast cancer cell lines in tryptophan metabolism-related gene expression (four differentially expressed genes: HAAO, KYNU, KMO, AFMID) and metabolite abundance (five differentially abundant metabolites). Integration analysis identified 3-hydroxyanthranilic acid, cinnabarinic acid, kynurenine, and kynurenic acid as potential metabolic biomarkers. These four metabolites differed significantly in serum of HCs, TS and TR patients. Four predictors were selected by LASSO, and a nomogram was constructed based on logistic regression. Finally, biomarkers and related genes were validated in tissues.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>Our explored the changes induced by trastuzumab resistance in terms of metabolite abundance and gene transcription levels associated with tryptophan metabolism. Furthermore, this work provided new insights into novel diagnostic biomarkers and prediction models of trastuzumab resistance in HER2-positive breast cancer patients.</p>\u0000 </section>\u0000 </div>","PeriodicalId":15509,"journal":{"name":"Journal of Clinical Laboratory Analysis","volume":"39 23","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jcla.70124","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145445061","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Epithelial-Mesenchymal Transition States and Metabolic Reprogramming Related Signatures Predict Prognosis and Therapeutic Responses in HER2-Positive Breast Cancer","authors":"Yuhui Zheng,&nbsp;Yinduo Zeng,&nbsp;Mengping Xi,&nbsp;Guoqiang Zhu,&nbsp;Jinyu Yang,&nbsp;Peng Cui,&nbsp;Guoqiang Wang,&nbsp;Yuzi Zhang,&nbsp;Chunwei Xu,&nbsp;Wenxian Wang,&nbsp;Shangli Cai,&nbsp;Li Wang,&nbsp;Xiaowen Wu","doi":"10.1002/jcla.70119","DOIUrl":"10.1002/jcla.70119","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Epithelial-mesenchymal transition (EMT) and metabolic reprogramming have been shown to regulate HER2 targeted therapy resistance and tumor metastasis. We aimed to establish an EMT-associated and metabolic-related prognostic model in HER2-positive breast cancer.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>mRNA expression and clinical information for HER2-positive breast cancer are downloaded from the TCGA database. The single sample gene set enrichment analysis score was used to generate EMT subtypes and identify EMT-related metabolic pathways. A prognostic risk score was developed based on the differentially expressed genes (DEGs) of the EMT-associated metabolic pathways using least absolute shrinkage and selection operator (LASSO) Cox regression and then validated in an external cohort (GSE96058).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>The EMT enriched scores differentiated the OS in the HER2-positive breast cancer TCGA cohort (<i>p</i> = 0.023; HR, 0.38; 95% CI, 0.16–0.9), which was also associated with the carbohydrate, amino acid, nucleotide, and tricarboxylic acid (TCA) cycle pathways (<i>p</i> &lt; 0.05). A total of 10 genes based on the DEGs between the metabolic groups were used to construct the prognosis model. Patients with low-risk metabolic scores showed longer OS compared to those with high-risk metabolic scores (<i>p</i> &lt; 0.001; HR, 0.09; 95% CI, 0.03–0.31). The association between OS and the metabolism score remained significant in the multivariable Cox regression (<i>p</i> &lt; 0.001; HR, 0.08; 95% CI, 0.03–0.22). Similar results were observed in the validation cohort (<i>p</i> = 0.002; HR, 0.34; 95% CI, 0.16–0.69).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>EMT and metabolic reprogramming-related features are specific prognostic and potential therapeutic biomarkers for HER2-positive breast cancer patients, which warrant further studies.</p>\u0000 </section>\u0000 </div>","PeriodicalId":15509,"journal":{"name":"Journal of Clinical Laboratory Analysis","volume":"39 22","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-11-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jcla.70119","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145431778","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigating the Factors Affecting the Commutability of Hemoglobin A1C Frozen Pooled Blood Materials","authors":"Kanokwan Ngueanchanthong,&nbsp;Napaporn Apiratmateekul,&nbsp;Supaporn Suparak,&nbsp;Nam K. Tran,&nbsp;Wanvisa Treebuphachatsakul","doi":"10.1002/jcla.70125","DOIUrl":"10.1002/jcla.70125","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Measurement of hemoglobin A_1C (HbA_1C) is essential for the diagnosis and treatment of diabetes mellitus (DM). Processed blood materials (PBMs), such as frozen pooled blood (FPB), are commonly used for External Quality Assessment (EQA). However, their commutability may be affected by factors such as hemolysis and matrix modification. This study evaluated FPB commutability and identified the factors influencing its performance in HbA_1C measurements.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>HbA_1C contents in 24 clinical blood samples (CBSs) and 10 PBMs, including FPBs, single-donor blood (SDB), and in vitro glycated blood (IGB), were analyzed using enzymatic (EN), capillary electrophoresis (CE), cation-exchange high-performance liquid chromatography (CE-HPLC), turbidimetric immunoassay (TI), and boronate affinity HPLC (BA-HPLC). EN served as the reference measurement procedure. Commutability was assessed using Deming regression and a 95% prediction interval. The hematocrit (HCT), visual appearance, plasma absorbance (PA), hemolysis index (HI), pH, and debris cells (DC) were analyzed.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>FPB1 and SDB were commutable across all measurement procedures. FPB2 and FPB4 were commutable with CE, CE-HPLC, and TI but non-commutable with BA-HPLC when HbA_1C was ≥ 6.23%, as data points fell outside the tolerance limits of the Deming regression lines. Non-commutable PBMs showed higher PA and HI along with lower pH. DC affected HbA_1C quantification using CE-HPLC.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>Hemolysis, as assessed by PA and HI, affects FPB commutability. These factors should be considered in EQA programs, particularly for BA-HPLC. HCT and DC did not directly affect commutability; however, maintaining appropriate HCT levels and monitoring DC-related chromatographic interference are crucial for ensuring FPB reliability in HbA_1C comparisons.</p>\u0000 </section>\u0000 </div>","PeriodicalId":15509,"journal":{"name":"Journal of Clinical Laboratory Analysis","volume":"39 23","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jcla.70125","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145421625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Laboratory Data Analysis of Hemorrhagic Fever With Renal Syndrome Patients to Predict Disease Severity and Patient Prognosis","authors":"Hong Shi,&nbsp;Feng Du,&nbsp;Ting Wang,&nbsp;Zhendong Gu,&nbsp;Ting Ruan,&nbsp;Qiujian Zhao,&nbsp;Rui Xu,&nbsp;Yi Wang,&nbsp;Langxi Luo,&nbsp;Shaohua Wang,&nbsp;Liejun Jiang,&nbsp;Yaoni Li","doi":"10.1002/jcla.70126","DOIUrl":"10.1002/jcla.70126","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Hemorrhagic fever with renal syndrome (HFRS) is an endemic disease occurring in various parts of the world. Prompt access to care with proper treatment is essential for preventing patients from developing renal failure and unfavorite outcomes. This study aimed to elucidate laboratory parameters associated with HFRS severity and prognosis to predict disease course and initiate prompt clinical management.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Retrospective analysis of laboratory data was performed on HFRS patients from December 2016 to January 2022 in Baoji City of Shaanxi Province in China using different statistical methods.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>The WBC and neutrophils in peripheral blood, RBC in urine sediments, blood, protein, and glucose in urine, PT/INR, aPTT, TT, AST, ALT, AST/ALT, MAO, AD, urea, creatinine, cystatin C, CK-MB, LDH, α-HBDH, mAST, triglycerides, glucose, amylase, ferritin, and PCT in serum increased in HFRS patients along with disease severity, while the lymphocytes, monocytes, platelets, plateletcrit, fibrinogen, serum total protein, albumin, HDL-c, magnesium, complement C3 and C4, IgG, triiodothyronine, thyroxine, and free triiodothyronine were reduced. Results also indicated that in uncured HFRS patients, the NEUT%, CRP, cast, PT/INR, aPTT, D-dimer, AST/ALT, CK-MB, LDH, α-HBDH, m-AST, ferritin, and PCT were significantly higher than in cured patients, while platelets, C3, and C4 in uncured patients were significantly lower than in cured patients. The NEUT%, CRP, AST/ALT, and LDH were associated with patients' prognosis.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>Laboratory data are helpful in predicting HFRS patients' progress, severity, and prognosis, thus, these parameters are useful in guiding prompt clinical management of patients.</p>\u0000 </section>\u0000 </div>","PeriodicalId":15509,"journal":{"name":"Journal of Clinical Laboratory Analysis","volume":"39 23","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-10-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jcla.70126","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145377772","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluating the “PLT Clumps?” Flag Originating From Different Channels of the Sysmex XN Haematology Analyser: A Method for Rapidly Distinguishing Between Two Preanalytical Factors Affecting PLT Counting for Cancer Patients","authors":"Danfei Xu,&nbsp;Na Zhao,&nbsp;Wenqi Tai,&nbsp;Jing Yang,&nbsp;Li Wang","doi":"10.1002/jcla.70111","DOIUrl":"10.1002/jcla.70111","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>This study investigated the accuracy of the “PLT Clumps?” flag triggered by different channels on the Sysmex XN haematology analyser in identifying two sample states and to optimize the platelet review strategy for cancer patients.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>570 samples flagged “PLT Clumps?” in CBC + DIFF mode were analysed. After excluding clots, retesting was conducted in CBC + DIFF + PLT-F mode. The accuracy of the “PLT Clumps?” flag and its correlation with PLT aggregation (PA) or fibrin precipitation (FP) were evaluated. Low-platelet count samples were collected for a second blood draw to confirm whether pseudothrombocytopenia (PTCP) was present. The incidence and positive predictive value (PPV) of the “PLT Clumps?” flag across different tumours were also analysed.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Among 85 verified cases (PA = 17, FP = 63), the overall PPV of the “PLT Clumps?” flag was 14.9% (85/570) in CBC + DIFF mode, 3.5% in WNR channel only, 73.9% in WDF channel only, and 94.4% in dual-channel. In CBC + DIFF + PLT-F mode, the overall PPV and NPV were 98.5% (65/66) and 97.0% (484/499), respectively. PA and FP accounted for 73.9% (17/23) and 26.1% (6/23) of the PLT-F-only flagged samples, respectively, and all WDF-only and dual-flagged cases were triggered by FP. The incidence of PTCP in samples flagged for “PLT Clumps?” in CBC + DIFF + PLT-F mode was 80%. Samples from patients with hepatobiliary tumours showed highest flagging rate (20.4%) but a lower PPV.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>The correlation between “PLT Clumps?” flags originating from different channels and two common preanalytical factors interfering in PLT counting was analysed, and the platelet review strategy for cancer patients was optimized.</p>\u0000 </section>\u0000 </div>","PeriodicalId":15509,"journal":{"name":"Journal of Clinical Laboratory Analysis","volume":"39 21","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jcla.70111","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145367977","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antifungal Resistance of Candida spp. Isolates From HIV/AIDS Patients in Iran 1999–2024: A Systematic Review and Meta-Analysis","authors":"Aliasghar Fakhri-Demeshghieh,&nbsp;Ali Yousefipour,&nbsp;Yousef Bolouki,&nbsp;Mohammad Sadegh Bigdeli,&nbsp;Erfan Zamani,&nbsp;Amirreza Farajkhoda,&nbsp;Alireza Salmani,&nbsp;Farzad Katiraee,&nbsp;Saied Bokaie","doi":"10.1002/jcla.70101","DOIUrl":"10.1002/jcla.70101","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Antifungal resistance in <i>Candida</i> species in HIV/AIDS patients due to the scarcity of antifungal agents is important particularly in Iran with an estimate of 46,000 individuals living with HIV. This systematic review and meta-analysis aimed to determine the resistance rate of <i>Candida</i> spp. isolates from HIV/AIDS positive individuals in Iran from 1999 to 2024.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>PubMed, Scopus, Web of Science, MagIran, Scientific Information Database, and Google Scholar were searched and the title-and-abstract screening was performed independently by two review authors. The risk of bias assessment and the data extraction were performed independently by two review authors. Meta-analysis was performed using a random-effects model with Freeman-Tukey double arcsine transformation. This review adhered to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses 2020.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>From the retrieved 4925 references, 18 references belonging to 15 studies with 1318 <i>Candida</i> spp. isolates were included. The highest pooled resistance rate of <i>Candida</i> spp. was observed against clotrimazole (21%, 95% CI: 14–30, <i>I</i>² = 67.29%) while the lowest pooled resistance rates of <i>Candida</i> spp. were observed against voriconazole, caspofungin, amphotericin B, and nystatin with the pooled estimates of 0%. At the species level, the highest pooled resistance rates were observed for <i>Candida krusei</i> against fluconazole (72%, 95% CI: 0%–100%) and itraconazole (38%, 95% CI: 17%–62%), and for <i>Candida glabrata</i> against clotrimazole (38%, 95% CI: 27%–50%).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>The use of amphotericin B, caspofungin, and nystatin for the treatment of <i>Candida</i> spp. in HIV-AIDS patients in Iran is recommended based on clinical indication.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Trial Registration</h3>\u0000 \u0000 <p>PROSPERO ID: CRD42024540580</p>\u0000 </section>\u0000 </div>","PeriodicalId":15509,"journal":{"name":"Journal of Clinical Laboratory Analysis","volume":"39 21","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jcla.70101","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145354892","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Predictive Value of Laboratory Assays Toward Autoimmune Diseases","authors":"Kellen J. Begeman,&nbsp;Mark J. White,&nbsp;Elizabeth S. Moore,&nbsp;Heidi H. Ewen","doi":"10.1002/jcla.70106","DOIUrl":"10.1002/jcla.70106","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objective</h3>\u0000 \u0000 <p>With an increasing number of unmet medical needs for autoimmune diseases (AD) in the United States, it is important to assess the value of laboratory assays in diagnosis. Healthcare providers must understand general and specific laboratory results that can lead to an efficient diagnosis.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>A nonexperimental study using a retrospective design and data collected between 2018 and 2021 was completed to explore whether the presence of positive anti-ENA and anti-dsDNA antibodies in individuals with an autoimmune disorder (anti-ENA, <i>N</i> = 1495; anti-dsDNA, <i>N</i> = 1261) can be predicted by their demographics and selected general laboratory test results.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>For predicting anti-ENA, multiple logistic regression analysis <i>χ</i>²(1) = 237.62, <i>p</i> &lt; 0.001 indicated antinuclear antibody (ANA), complement C3, globulin, monocyte-to-lymphocyte ratio (MLR), and blood urea nitrogen (BUN) were statistically associated with the probability of a positive anti-ENA result. The regression analysis had a sensitivity of 98.6% and a specificity measure of 16.1%. Regarding anti-dsDNA, multiple logistic regression analysis <i>χ</i>²(1) = 388.04, <i>p</i> &lt; 0.001, indicated that complement C3, complement C4, pH, urine protein, and neutrophil-to-lymphocyte ratio (NLR) were statistically associated with the probability of a positive anti-dsDNA result. The regression analysis had a sensitivity of 77.7% and a specificity measure of 64.5%.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>In diagnosing an AD, ANA, complement C3, globulin, MLR, and BUN are important factors in patients with a positive anti-ENA. In determining Systemic Lupus Erythematosus diagnosis, complement C3, complement C4, pH, urine protein, and NLR are important factors in patients with a positive anti-dsDNA.</p>\u0000 </section>\u0000 </div>","PeriodicalId":15509,"journal":{"name":"Journal of Clinical Laboratory Analysis","volume":"39 21","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jcla.70106","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145354901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quantitative Analysis of DNA Double-Strand Breaks in Genomic DNA Using Standard Curve Method","authors":"Lihuang Guo,&nbsp;Hanying Dai,&nbsp;Jiancheng Li,&nbsp;Chenwei Li,&nbsp;Yue Huang,&nbsp;Keqian Xu","doi":"10.1002/jcla.70123","DOIUrl":"10.1002/jcla.70123","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>DNA double-strand breaks (DSBs) are the most lethal and dangerous type of lesions with significant implications for both cellular function and organismal health. The number of DSBs (N_DSBs) across the genome reflects DNA damage severity. However, current quantification methods mainly rely on next-generation sequencing, which is laborious and expensive. This study aims to provide a simple, low-cost, and high-throughput standard curve-based method for quantifying genome-wide DSBs.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Method</h3>\u0000 \u0000 <p>Genomic DNA from human, mouse, <i>Arabidopsis</i>, <i>Saccharomyces cerevisiae</i>, and <i>Escherichia coli</i> was digested by seven blunt-end restriction enzymes to generate DSB standards. Theoretical N_DSBs for each standard were calculated based on restriction site frequency. Ligation-mediated quantitative PCR (LM-qPCR) was performed to obtain the Ct values, which were plotted against log-transformed N_DSBs to construct standard curves. Method reliability was assessed by comparing results with neutral single-cell gel electrophoresis and γ-H2AX flow cytometry.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>All genomes were successfully digested by seven blunt-end restriction enzymes to produce standard DSB fragments. Standard curves demonstrated high linearity (<i>R</i>² &gt; 0.95), with intra- and inter-assay coefficients of variation of 1.101% and 2.528%, respectively. The detection limit was below 100 DSBs. Quantification results strongly correlated with traditional DSB detection methods (|<i>r</i>| &gt; 0.9).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>This standard curve-based method enables accurate, reproducible quantification of genome-wide DSBs in various organisms. It is simple, low-cost, and easily standardized, offering a promising tool for applications in genotoxicity testing, environmental exposure monitoring, and DNA damage research.</p>\u0000 </section>\u0000 </div>","PeriodicalId":15509,"journal":{"name":"Journal of Clinical Laboratory Analysis","volume":"39 23","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jcla.70123","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145312165","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detection of blaNDM-1 and blaIMP-1 Metallo-β-Lactamases in Meropenem-Resistant Clinical Escherichia coli Isolates Using Modified Carbapenem Inactivation Method (mCIM) and PCR Techniques","authors":"Mehdi Roshdi Maleki","doi":"10.1002/jcla.70100","DOIUrl":"10.1002/jcla.70100","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Introduction</h3>\u0000 \u0000 <p><i>Escherichia coli</i>, a member of the <i>Enterobacteriaceae</i> family, is a leading cause of various infections. Carbapenems, a potent class of β-lactam antibiotics, serve as the last line of defense against multidrug-resistant bacteria. However, the global rise of carbapenemase-producing <i>E. coli</i> (CP-Ec), particularly metallo-β-lactamases (MBLs), poses a significant public health concern. This study aimed to determine the prevalence of <i>bla</i>_NDM-1 and <i>bla</i>_IMP-1 genes in <i>E. coli</i> strains isolated from clinical samples.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>A cross-sectional study was conducted on 80 meropenem-resistant <i>E. coli</i> strains isolated from clinical samples. Bacterial identification was performed using biochemical tests, including IMViC. Carbapenemase production was assessed using the modified carbapenem inactivation method (mCIM). Genotypic analysis of carbapenem-resistant strains was conducted via polymerase chain reaction (PCR) to detect <i>bla</i>_NDM-1 and <i>bla</i>_IMP-1 genes.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>The mCIM phenotypic test identified 46.25% (37/80) of isolates as carbapenemase producers. PCR analysis revealed that <i>bla</i>_NDM-1 was more prevalent than <i>bla</i>_IMP-1 (48.65% vs. 16.22%). Four isolates (10.81%) carried both genes. Notably, 35.14% (13/37) of meropenem-resistant <i>E. coli</i> isolates tested negative for both <i>bla</i>_NDM-1 and <i>bla</i>_IMP-1, indicating the possible involvement of other carbapenemase genes such as <i>bla</i>_VIM or non-carbapenemase mechanisms.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>The detection of <i>bla</i>_NDM-1 and <i>bla</i>_IMP-1 genes in <i>E. coli</i> highlights the alarming spread of carbapenem resistance. The presence of resistant strains lacking these genes suggests additional resistance mechanisms, warranting further investigation.</p>\u0000 </section>\u0000 </div>","PeriodicalId":15509,"journal":{"name":"Journal of Clinical Laboratory Analysis","volume":"39 21","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jcla.70100","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145308201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Severe Malnutrition Identified by the Controlling Nutritional Status (CONUT) Score Is Associated With Prolonged Intensive Care Unit (ICU) Stay in Pneumonia Complicated With Respiratory Failure Patients Who Underwent Invasive Mechanical Ventilation","authors":"Zhijuan Zheng,&nbsp;Guixia Peng,&nbsp;Yue Xiao","doi":"10.1002/jcla.70109","DOIUrl":"10.1002/jcla.70109","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Mechanical ventilation is an effective method to improve the ventilation of patients with severe pneumonia and respiratory failure. The length of intensive care unit (ICU) stay reflects the treatment effectiveness of patients. This study was to evaluate the relationship between Controlled Nutritional Status (CONUT) score and prolonged ICU stay in patients with pneumonia complicated by respiratory failure who underwent invasive mechanical ventilation.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>1994 patients who underwent invasive mechanical ventilation were retrospectively analyzed. Medical records (age, gender, body mass index, smoking, drinking, hypertension, diabetes mellitus, lung diseases, blood transfusion, and serum albumin, lymphocyte, cholesterol levels) were collected. The threshold for prolonged ICU stay was defined based on the third quartile (75th percentile) of length of ICU stay. The relationship between CONUT and prolonged ICU stay was analyzed.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>The mean ICU stay of patients was 6.6 (3.9, 11.6) days; there were 1495 (75.0%) patients without prolonged ICU stay (&lt; 11.6 days) and 499 (25.0%) with prolonged ICU stay (≥ 11.6 days). The proportion of CONUT severe grade in patients with prolonged ICU stay was higher than that in patients without prolonged ICU stay. Logistic regression analysis showed that CONUT severe grade (odds ratio (OR): 1.298, 95% confidence interval (CI): 1.024–1.646, <i>p</i> = 0.031), smoking (OR: 1.475, 95% CI: 1.105–1.968, <i>p</i> = 0.008), and blood transfusion (OR: 2.981, 95% CI: 2.406–3.694, <i>p</i> &lt; 0.001) were independently associated with prolonged ICU stay.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>CONUT severe grade, smoking, and blood transfusion were independently associated with prolonged ICU stay in pneumonia with respiratory failure patients who underwent invasive mechanical ventilation.</p>\u0000 </section>\u0000 </div>","PeriodicalId":15509,"journal":{"name":"Journal of Clinical Laboratory Analysis","volume":"39 21","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jcla.70109","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145286184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}