Journal of Clinical Laboratory Analysis最新文献_第6页

Prediction the Occurrence of Thalassemia With Hematological Phenotype by Diagnosis of Abnormal HbA1c 通过诊断异常 HbA1c 预测地中海贫血症的血液学表型。

IF 2.6 4区医学 Q2 MEDICAL LABORATORY TECHNOLOGY

Journal of Clinical Laboratory Analysis

Pub Date : 2024-09-24 DOI: 10.1002/jcla.25104

Yafang Wan, Yu Zhang, Tian Li, Shuyue Chen, Changchun Niu

Background

The current investigation aims to analyze the occurrence of thalassemia in patients who participated in hemoglobin A1c (HbA1c) testing in clinical laboratory showing high hemoglobin F (HbF) level (≥ 1.5%) or abnormal Hb peak and predict the main influence factors by using different statistical models.

Methods

The current investigation is a single-center retrospective cohort study. HbA1c concentration was detected by using TOSOH HLC-723G8 glycated hemoglobin analyzer. SNaPshot SNP (Single Nucleotide Polymorphism) typing and AccuCopy technology were employed to detect mutations in thalassemia-related pathogenic genes.

Results

A total of 126 patients endured high HbF levels or abnormal Hb peak during HbA1c detection, and 66.7% of subjects (n = 84) showed thalassemia mutations. Three heterozygosity mutations, including c.52A>T (p.K18*), c.-78A>G, and c.126_129delCTTT(p.F42Lfs*19) present in HBB gene, were also identified. --^SEA/αα mutation demonstrated the youngest ages (p < 0.001). 17 M (p < 0.001) and 41/42 M (p < 0.01) mutations with β-thalassemia showed higher HbF levels compared with patients without thalassemia mutations. Except for -α^3.7, mutations in thalassemia showed lower levels of mean corpuscular hemoglobin (MCH) and mean corpuscular volume (MCV) compared with patients without thalassemia mutations. Patients with thalassemia mutations showed younger age (p < 0.001), lower Hb (p < 0.001), MCV and MCH levels (p < 0.001), higher red blood cell (RBC) count (p < 0.001), and platelet distribution width (PDW) level (p = 0.007) than patients without thalassemia mutations. Three statistical models indicate MCV is the most valuable independent factor for predicting thalassemia and ROC (receiver operating characteristic) curves analysis of AUC (Area Under the Curve) of 0.855 (95% CI [0.787–0.923], p < 0.001) with MCV.

Conclusion

High HbF level (≥ 1.5%) or abnormal Hb peak present in HbA1c testing indicated high incident rate of thalassemia. MCV is the most valuable independent predicting factor for subjects having thalassemia.

研究背景本次调查旨在分析在临床实验室参加血红蛋白A1c（HbA1c）检测并显示血红蛋白F（HbF）水平高（≥1.5%）或Hb峰值异常的患者中地中海贫血的发生率，并通过不同的统计模型预测主要影响因素：本次调查是一项单中心回顾性队列研究。方法：本研究为单中心回顾性队列研究，使用 TOSOH HLC-723G8 糖化血红蛋白分析仪检测 HbA1c 浓度。采用 SNaPshot SNP（单核苷酸多态性）分型和 AccuCopy 技术检测地中海贫血相关致病基因的突变：结果：共有 126 名患者在检测 HbA1c 时出现 HbF 水平过高或 Hb 峰值异常，66.7% 的受试者（n = 84）出现地中海贫血基因突变。此外，还发现了三种杂合突变，包括 HBB 基因中的 c.52A>T (p.K18*)、c.-78A>G 和 c.126_129delCTTT(p.F42Lfs*19)。--与没有地中海贫血基因突变的患者相比，地中海贫血基因突变患者的平均血红蛋白（MCH）和平均血红蛋白容积（MCV）水平较低（p.3.7）。地中海贫血突变患者的年龄更小（p 结论：地中海贫血突变患者的年龄更小（p 结论：地中海贫血突变患者的年龄更小（p 结论）：高 HbF 水平（≥ 1.5%）或 HbA1c 检测中出现异常 Hb 峰表明地中海贫血发病率高。MCV 是地中海贫血症患者最有价值的独立预测因素。

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引用次数: 0

Clinical Utility of Circulating Tumor DNA for Detecting Lung Cancer Mutations by Targeted Next-Generation Sequencing With Insufficient Tumor Samples 在肿瘤样本不足的情况下，利用循环肿瘤DNA通过靶向新一代测序检测肺癌突变的临床实用性

IF 2.6 4区医学 Q2 MEDICAL LABORATORY TECHNOLOGY

Journal of Clinical Laboratory Analysis

Pub Date : 2024-09-24 DOI: 10.1002/jcla.25099

Yi Sun, Xu Zhang, Xinhua Yang, Jiangjun Ma

Background

Circulating tumor deoxyribonucleic acid (ctDNA) is increasingly applied in clinical practice. This study aimed to explore clinical utility of a minimal invasive and sensitive way of ctDNA for next-generation sequencing in non–small cell lung cancer (NSCLC) with inadequate tumor samples.

Methods

Targeted DNA sequencing was performed on tissue biopsies and matched plasma samples from 60 patients with NSCLC.

Results

A total of 13 driving genes were detected in 60 matched tissue DNA (tDNA) and ctDNA samples. Overall concordance rate was 75.47%, with 77.55% sensitivity and 50% specificity. Epidermal growth factor receptor (EGFR) mutations were the most common in both tDNA and ctDNA samples. Among other mutated genes were tumor protein p53 (TP53), erb-b2 receptor tyrosine kinase 2 (ERBB2), anaplastic lymphoma kinase (ALK), cyclin-dependent kinase inhibitor 2A (CDKN2A), ros proto-oncogene 1, and receptor tyrosine kinase (ROS1). Mutations in b-raf proto-oncogene, serine/threonine kinase (BRAF), cluster of differentiation 274 (CD274), neurotrophin receptor tyrosine kinase 1 (NTRK1), and rearranged during transfection (RET) occurred only in plasma. The majority of mutations in both samples were single-nucleotide variants. Deletions were found in EGFR, BRAF, and TP53 in ctDNA, whereas in tDNA, deletions were only found in EGFR. In ALK, single nucleic acid-site amplification occurred simultaneously in tissue and plasma, but insertions and copy number variations were detected only in plasma.

Conclusions

Identifying ctDNA mutations by targeted sequencing in plasma is feasible, showing the clinical value of ctDNA-targeted sequencing in NSCLC patients when tumor tissue sampling is insufficient or even impossible.

背景：循环肿瘤脱氧核糖核酸（ctDNA循环肿瘤脱氧核糖核酸（ctDNA）越来越多地应用于临床实践。本研究旨在探索在肿瘤样本不足的非小细胞肺癌（NSCLC）中用微创、灵敏的方法对ctDNA进行下一代测序的临床实用性：方法：对60名NSCLC患者的组织活检样本和匹配的血浆样本进行靶向DNA测序：结果：在60份匹配的组织DNA（tDNA）和ctDNA样本中，共检测到13个驱动基因。总体吻合率为 75.47%，灵敏度为 77.55%，特异度为 50%。表皮生长因子受体（EGFR）突变在 tDNA 和 ctDNA 样本中最为常见。其他突变基因包括肿瘤蛋白 p53 (TP53)、ERBB2 受体酪氨酸激酶 2 (ERBB2)、无性淋巴瘤激酶 (ALK)、细胞周期蛋白依赖性激酶抑制剂 2A (CDKN2A)、ros 原癌基因 1 和受体酪氨酸激酶 (ROS1)。b-raf原癌基因、丝氨酸/苏氨酸激酶（BRAF）、分化簇274（CD274）、神经营养素受体酪氨酸激酶1（NTRK1）和转染时重排（RET）的突变只发生在血浆中。两个样本中的大多数突变都是单核苷酸变异。在ctDNA中发现了表皮生长因子受体、BRAF和TP53的缺失，而在tDNA中只发现了表皮生长因子受体的缺失。在ALK中，单核酸位点扩增同时出现在组织和血浆中，但插入和拷贝数变异仅在血浆中检测到：结论：在血浆中通过靶向测序鉴定ctDNA突变是可行的，这表明在肿瘤组织取样不足甚至不可能的情况下，ctDNA靶向测序在NSCLC患者中具有临床价值。

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引用次数: 0

Serum Procalcitonin, Hematology Parameters, and Cell Morphology in Multiple Clinical Conditions and Sepsis 多种临床症状和败血症中的血清降钙素原、血液学参数和细胞形态。

IF 2.6 4区医学 Q2 MEDICAL LABORATORY TECHNOLOGY

Journal of Clinical Laboratory Analysis

Pub Date : 2024-09-21 DOI: 10.1002/jcla.25100

Pingfeng Feng, Yongjian He, Ping Guan, Chaohui Duan, Junjie Huang, Zhixin Chai, Jingjing Wang, Huifei Zheng, Junxu Luo, Yuhuan Shi, Xin Li, Huayi Huang

Background

The clinical value of procalcitonin (PCT) in infection diagnosis and antibiotic stewardship is still unclear. This study aimed to investigate the association between serum PCT and different clinical conditions as well as other infectious/inflammatory parameters in different septic patients in order to elucidate the value of PCT detection in infection management.

Methods

Chemiluminescence immunoassay was used for serum PCT analysis. Hematology analysis was used for complete blood cell count. Digital automated cell morphology analysis was used for blood cell morphology examination. Blood, urine, and stool cultures were performed according to routine clinical laboratory standard operating procedures. C-reactive protein (CRP) was analyzed by immunoturbidimetry. Erythrocyte sedimentation rate test was performed using natural sedimentation methods.

Results

Outpatients, ICU patients, and patients under 2 years of age with respiratory infections had higher serum PCT levels. Septic patients had the highest-serum PCT levels and other infection indexes. PCT levels in the blood, urine, and stool culture-positive patients were significantly higher than in culture-negative patients. The neutrophil granulation and reactive lymphocytes were observed together with the PCT-level increments in different septic patients, and these alterations were lessened after treatment. There was no significant change in monocyte morphology between pre- and posttreatment septic patients.

Conclusions

Serum PCT is associated with neutrophil cytotoxicity and lymphocyte morphology changes in sepsis; thus, the combination of neutrophil and lymphocyte digital cell morphology evaluations with PCT detection may be a useful examination for guiding the clinical management of sepsis.

背景：降钙素原（PCT）在感染诊断和抗生素管理中的临床价值尚不明确。本研究旨在调查不同脓毒症患者血清 PCT 与不同临床症状及其他感染/炎症参数之间的关联，以阐明 PCT 检测在感染管理中的价值：方法：采用化学发光免疫测定法分析血清PCT。血液学分析用于全血细胞计数。数字自动细胞形态分析用于血细胞形态检查。血液、尿液和粪便培养按照常规临床实验室标准操作程序进行。C反应蛋白（CRP）采用免疫比浊法进行分析。红细胞沉降率检测采用自然沉降法：结果：门诊病人、重症监护室病人和两岁以下呼吸道感染病人的血清 PCT 水平较高。败血症患者的血清 PCT 水平和其他感染指数最高。血液、尿液和粪便培养阳性患者的 PCT 水平明显高于培养阴性患者。在不同的脓毒症患者中，中性粒细胞肉芽肿和反应性淋巴细胞与 PCT 水平的升高同时出现，这些变化在治疗后有所减轻。治疗前后的脓毒症患者单核细胞形态无明显变化：血清 PCT 与脓毒症患者的中性粒细胞细胞毒性和淋巴细胞形态变化有关；因此，将中性粒细胞和淋巴细胞数字细胞形态评估与 PCT 检测相结合，可能是指导脓毒症临床治疗的有效检查方法。

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引用次数: 0

Characteristics of Candida albicans Derived From HIV-Positive Individuals With Oral Candidiasis: Genotyping, Phenotypic Variation, Antifungal Susceptibility, and Biofilm Formation 从患有口腔念珠菌病的 HIV 阳性个体中提取的白色念珠菌的特征：基因分型、表型变异、抗真菌敏感性和生物膜形成。

IF 2.6 4区医学 Q2 MEDICAL LABORATORY TECHNOLOGY

Journal of Clinical Laboratory Analysis

Pub Date : 2024-09-19 DOI: 10.1002/jcla.25103

Maryam Erfaninejad, Ali Zarei Mahmoudabadi, Mohammad Hashemzadeh, Elham Maraghi, Mahnaz Fatahinia

Background

Oral candidiasis (OC) is one of the most common mucosal infections in those afflicted with HIV/AIDS. This study aimed to provide detailed information on the phenotype, genotype, antifungal susceptibility, and biofilm formation ability of oral Candida albicans isolated from HIV-infected patients with OC.

Methods

A total of 25 C. albicans isolates were collected from oral lesions of HIV-infected patients referred to Behavioral Diseases Counseling Center affiliated with Ahvaz Jundishapur University of Medical Sciences, Iran. The antifungal susceptibility testing was done according to CLSI M27 guideline (fourth edition). The crystal violet method was used to evaluate the biofilm formation ability of isolates. Different phenotypes were identified on yeast extract-peptone-dextrose agar medium supplemented with phloxine B. Genotyping analysis of the isolates was performed using high-resolution melting (HRM) assays and ABC genotyping.

Results

The highest and lowest susceptibility of the C. albicans isolates was found for fluconazole 24 (96%) and ITC 18 (72%), respectively. Forty-eight percent of the isolates had high biofilm formation ability and exhibited gray cell type. The most common genotype was genotype B (52%). HRM analysis of HIS3, EF3, and CDC3 markers showed three, four, and five different groups, respectively.

Conclusion

Investigating the phenotype, antifungal susceptibility and biofilm formation ability of the C. albicans isolates obtained from oral lesions of HIV-infected patients revealed that the dominant genotypes in the current research could cause more serious infections from the oral source. We recommend further research with a larger sample size to determine the molecular epidemiology of C. albicans among HIV patients in Iran.

背景：口腔念珠菌病（OC）是艾滋病毒/艾滋病患者最常见的粘膜感染之一。本研究旨在提供从 OC 患者中分离出的口腔白色念珠菌的表型、基因型、抗真菌敏感性和生物膜形成能力的详细信息：方法：从转诊至伊朗 Ahvaz Jundishapur 医科大学附属行为疾病咨询中心的 HIV 感染者口腔病变中收集了 25 株白色念珠菌。抗真菌药敏试验根据 CLSI M27 指南（第四版）进行。水晶紫法用于评估分离菌的生物膜形成能力。使用高分辨率熔融（HRM）测定法和 ABC 基因分型法对分离菌株进行基因分型分析：结果：白僵菌分离株对氟康唑的敏感性最高，为 24 株（96%），对 ITC 的敏感性最低，为 18 株（72%）。48%的分离株具有较强的生物膜形成能力，并表现为灰色细胞型。最常见的基因型是基因型 B（52%）。HIS3、EF3 和 CDC3 标记的 HRM 分析分别显示出三个、四个和五个不同的组别：对从艾滋病病毒感染者口腔病变中分离出的白僵菌的表型、抗真菌敏感性和生物膜形成能力进行调查后发现，目前研究中的优势基因型可能会导致更严重的口腔源感染。我们建议进一步开展样本量更大的研究，以确定伊朗艾滋病患者中白念珠菌的分子流行病学。

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引用次数: 0

Evaluation of a Chemiluminescent Enzyme Immunoassay for the Detection of Prostaglandin E-Major Urinary Metabolite (PGE-MUM) 评估用于检测前列腺素 E-主要尿液代谢物 (PGE-MUM) 的化学发光酶免疫测定。

IF 2.6 4区医学 Q2 MEDICAL LABORATORY TECHNOLOGY

Journal of Clinical Laboratory Analysis

Pub Date : 2024-09-16 DOI: 10.1002/jcla.25102

Jun Takagi, Kazushige Moriyama, Seiji Arihiro, Tomohiro Kato, Toshiyuki Sakurai, Masayuki Saruta, Mie Wakabayashi, Hidekazu Nasu, Noriko Katagiri, Shintaro Yagi

Background

We developed a fully automated quantitative immunoassay for the detection of prostaglandin E-major urinary metabolite (PGE-MUM). In this study, we evaluated the analytical performance of this assay.

Methods

Sensitivity, within-run reproducibility, correlation with radioimmunoassay (RIA), cross-reactivity, dilution linearity, spike recovery performance, analyte stability, and effects of coexisting substances were evaluated. The assay was also used to measure PGE-MUM in 211 healthy people.

Results

The limit of detection and quantification were 1.0 and 1.3 ng/mL, respectively. When the assay was performed six times in a single run, the coefficient of variation ranged from 1.4% to 2.2%. The coefficient of correlation with a preceding RIA method was 0.970 with a correlation slope of 0.88. There was no cross-reactivity with PGE-MUM analogs. Linearity of dilution was confirmed at up to 16-fold dilution with assay results within 100 ± 20% of the theoretical values calculated based on the undiluted sample. Spike recovery was good and ranged from 94% to 101%. Analyte stability was tested by storing samples at 25°C for 6 days, 10°C for 1 month, and by performing up to five freeze–thaw cycles. Assay results were all within 100 ± 10%, the values measured before storage and before the freeze–thaw process. Assay results in healthy people ranged from 3.1 to 162.7 ng/mL (mean: 35.8 ng/mL). After correction for creatinine, the 95% confidence interval was 8.68–42.25 μg/g creatinine.

Conclusion

The assay precisely detects PGE-MUM.

背景：我们开发了一种用于检测前列腺素 E 主要尿液代谢物（PGE-MUM）的全自动定量免疫分析法。在本研究中，我们评估了该检测方法的分析性能：方法：评估了灵敏度、运行内重现性、与放射免疫分析法（RIA）的相关性、交叉反应、稀释线性、加标回收率、分析物稳定性以及共存物质的影响。该方法还用于检测 211 名健康人体内的 PGE-MUM：结果：检测限和定量限分别为 1.0 和 1.3 纳克/毫升。在一次运行中进行六次测定时，变异系数在 1.4% 至 2.2% 之间。与之前的 RIA 方法的相关系数为 0.970，相关斜率为 0.88。与 PGE-MUM 类似物没有交叉反应。稀释倍数高达 16 倍时的线性关系得到了证实，检测结果在根据未稀释样品计算出的理论值的 100 ± 20% 范围内。加标回收率良好，在 94% 至 101% 之间。通过将样品在 25°C 下储存 6 天、10°C 下储存 1 个月以及进行多达 5 次冻融循环，测试了分析物的稳定性。化验结果均在 100 ± 10% 的范围内，即储存前和冻融前的测量值。健康人的检测结果介于 3.1 至 162.7 纳克/毫升（平均值：35.8 纳克/毫升）之间。对肌酐进行校正后，95% 的置信区间为 8.68-42.25 μg/g 肌酐：结论：该检测方法可精确检测 PGE-MUM。

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引用次数: 0

Evaluating the Association of Anthropometric Indices With Total Cholesterol in a Large Population Using Data Mining Algorithms 利用数据挖掘算法评估大量人群中人体测量指标与总胆固醇的关联性

IF 2.6 4区医学 Q2 MEDICAL LABORATORY TECHNOLOGY

Journal of Clinical Laboratory Analysis

Pub Date : 2024-09-13 DOI: 10.1002/jcla.25095

Sahar Arab Yousefabadi, Somayeh Ghiasi Hafezi, Alireza Kooshki, Marzieh Hosseini, Amin Mansoori, Mark Ghamsary, Habibollah Esmaily, Majid Ghayour-Mobarhan

Background

Unbalanced levels of serum total cholesterol (TC) and its subgroups are called dyslipidemia. Several anthropometric indices have been developed to provide a more accurate assessment of body shape and the health risks associated with obesity. In this study, we used the random forest model (RF), decision tree (DT), and logistic regression (LR) to predict total cholesterol based on new anthropometric indices in a sex-stratified analysis.

Method

Our sample size was 9639 people in which anthropometric parameters were measured for the participants and data regarding the demographic and laboratory data were obtained. Aiding the machine learning, DT, LR, and RF were drawn to build a measurement prediction model.

Results

Anthropometric and other related variables were compared between both TC <200 and TC ≥200 groups. In both males and females, Lipid Accumulation Product (LAP) had the greatest effect on the risk of TC increase. According to results of the RF model, LAP and Visceral Adiposity Index (VAI) were significant variables for men. VAI also had a stronger correlation with HDL-C and triglyceride. We identified specific anthropometric thresholds based on DT analysis that could be used to classify individuals at high or low risk of elevated TC levels. The RF model determined that the most important variables for both genders were VAI and LAP.

Conclusion

We tend to present a picture of the Persian population's anthropometric factors and their association with TC level and possible risk factors. Various anthropometric indices indicated different predictive power for TC levels in the Persian population.

背景血清总胆固醇（TC）水平不平衡及其亚群被称为血脂异常。为了更准确地评估体形和与肥胖相关的健康风险，人们开发了多种人体测量指数。在这项研究中，我们使用随机森林模型（RF）、决策树（DT）和逻辑回归（LR），在性别分层分析中根据新的人体测量指数预测总胆固醇。结果比较了 TC <200 组和 TC ≥200 组的人体测量指标和其他相关变量。在男性和女性中，脂质累积产物（LAP）对 TC 升高风险的影响最大。根据 RF 模型的结果，LAP 和内脏脂肪指数（VAI）是男性的重要变量。内脏脂肪指数与高密度脂蛋白胆固醇和甘油三酯的相关性也更强。我们根据 DT 分析确定了特定的人体测量阈值，可用于对 TC 水平升高的高危或低危人群进行分类。RF 模型确定，对男女两性而言，最重要的变量是 VAI 和 LAP。各种人体测量指数对波斯人口的血 TC 水平具有不同的预测能力。

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引用次数: 0

Chromosome 1q21 Aberrations Are Poor Prognostic Factors for Newly Diagnosed Multiple Myeloma Patients 染色体 1q21 畸变是新诊断多发性骨髓瘤患者的不良预后因素

IF 2.6 4区医学 Q2 MEDICAL LABORATORY TECHNOLOGY

Journal of Clinical Laboratory Analysis

Pub Date : 2024-09-12 DOI: 10.1002/jcla.25072

Tongtong Wang, Chuanying Geng, Guangzhong Yang, Huixing Zhou, Zhiyao Zhang, Yuan Jian, Wenming Chen

Background

Chromosome 1q21 aberrations are one of the most common cytogenetic abnormalities in patients with multiple myeloma (MM). However, the prognostic value remains controversial. This study aimed to determine the prognostic value of numerical abnormalities of chromosome 1q21 for newly diagnosed patients with MM patients in Chinese population.

Methods

We retrospectively analyzed 629 patients with newly diagnosed MM who received the detection of chromosome 1q21 by fluorescence in situ hybridization in China.

Results

Among 629 patients, 309 (49.1%) had 1q21 abnormalities, of which 187 (29.7%) had three copies and 122 (19.4%) had four or more copies. Patients with two copies of 1q21 had a significantly longer median overall survival (OS) than those with three copies or ≥4 copies and also had longer progression-free survival (PFS). However, patients with three or ≥4 copies had similar OS and PFS. Univariate Cox proportional hazards regression analyses determined that 1q21 aberrations are associated with shorter OS and PFS. 1q21 aberrations are also independent poor prognostic factors for OS and PFS in multivariable analyses. Del(17p), t(4;14), and t(14;16) are common high-risk cytogenetic abnormalities (HRCAs) in patients with MM. Patients with 1q21+ alone or 1q21+ combined with HRCAs had shorter OS and PFS than patients without cytogenetic abnormalities. Patients with 1q21+ and t(11;14) also had shorter PFS but had similar OS than patients without cytogenetic abnormalities.

Conclusion

Our study showed that chromosome 1q21 aberrations are poor prognostic factors for newly diagnosed patients with MM.

背景1q21染色体畸变是多发性骨髓瘤（MM）患者最常见的细胞遗传学异常之一。然而，其预后价值仍存在争议。本研究旨在确定中国人群中新诊断的 MM 患者 1q21 染色体数值异常的预后价值。方法我们回顾性分析了中国 629 例接受荧光原位杂交检测 1q21 染色体的新诊断 MM 患者。1q21有两个拷贝的患者的中位总生存期（OS）明显长于有三个拷贝或≥4个拷贝的患者，无进展生存期（PFS）也更长。然而，3个拷贝或≥4个拷贝的患者的OS和PFS相似。单变量考克斯比例危险回归分析确定，1q21畸变与较短的OS和PFS相关。在多变量分析中，1q21畸变也是OS和PFS的独立不良预后因素。Del(17p)、t(4;14)和t(14;16)是MM患者常见的高危细胞遗传学异常（HRCA）。与无细胞遗传学异常的患者相比，仅有1q21+或1q21+合并HRCA的患者的OS和PFS较短。结论：我们的研究表明，1q21染色体畸变是新诊断的MM患者的不良预后因素。

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引用次数: 0

Implicit Flow Cytometric Diagnosis of Classic Hodgkin Lymphoma Using CD3+CD4+CD26− T-Cells 利用 CD3+CD4+CD26- T 细胞对典型霍奇金淋巴瘤进行隐性流式细胞术诊断

IF 2.6 4区医学 Q2 MEDICAL LABORATORY TECHNOLOGY

Journal of Clinical Laboratory Analysis

Pub Date : 2024-09-05 DOI: 10.1002/jcla.25096

Curtis Gravenmier, Jinming Song, Haipeng Shao

Background

Flow cytometry is not routinely performed in clinical laboratories for the diagnosis of classic Hodgkin lymphoma (CHL).

Methods

Fourteen cases of CHL and 132 cases of the control group were studied by 10-color flow cytometry, with markers including CD3, CD4, CD7, CD8, and CD26, as well as calculated parameters such as the CD4:CD8 ratio, percent CD3⁺CD4⁺CD26⁻ T-cells of CD3⁺CD4⁺ T-cells, percent CD3⁺CD4⁺CD26⁻ T-cells of total events, CD7 coefficient of variation among CD3⁺CD4⁺CD26⁻ T-cells, and CD7 median fluorescence intensity of CD3⁺CD4⁺CD26⁻ T-cells relative to CD3⁺CD8⁺ T-cells.

Results

CHL cases showed a median percent CD3⁺CD4⁺CD26⁻ of CD3⁺CD4⁺ T-cells of 72.3% with range from 41.1% to 94.4%, median percent CD3⁺CD4⁺CD26⁻ T-cells of total events of 17.4% with range from 4.6% to 52.5%, CD7 coefficient of variation among CD3⁺CD4⁺CD26⁻ T-cells less than 100%, and CD7 median fluorescence intensity of CD3⁺CD4⁺CD26⁻ T-cells relative to CD3⁺CD8⁺ T-cells of 1.7 with range from 0.4 to 3.5. In the control group, every entity showed some degree of overlap with CHL in terms of these parameters. A “Hodgkin score” was thus constructed to enhance separation of CHL from other entities. A threshold Hodgkin score of 15.35 achieved a sensitivity of 78.6% and specificity of 96.2% in the diagnosis of CHL. Incorporating the Hodgkin score into a simple algorithm raises the specificity to 100%.

Conclusion

In this study, we used flow cytometry to demonstrate increased CD3⁺CD4⁺CD26⁻ T-cells in CHL, and derived a Hodgkin score for the diagnosis of CHL.

背景：流式细胞术并非临床实验室诊断典型霍奇金淋巴瘤（CHL）的常规方法：流式细胞术并非临床实验室诊断典型霍奇金淋巴瘤（CHL）的常规方法：14例CHL病例和132例对照组病例采用10色流式细胞术进行研究，标记物包括CD3、CD4、CD7、CD8和CD26，以及计算参数，如CD4：CD8比值、CD3+CD4+CD26- T细胞占CD3+CD4+ T细胞的百分比、CD3+CD4+CD26- T细胞占总事件的百分比、CD3+CD4+CD26- T细胞间的CD7变异系数、CD3+CD4+CD26- T细胞相对于CD3+CD8+ T细胞的CD7中位荧光强度等参数。结果显示CHL病例中 CD3+CD4+CD26- T细胞占 CD3+CD4+ T细胞的百分比中位数为 72.3%，范围在 41.1%至 94.4%之间；CD3+CD4+CD26- T细胞占总事件的百分比中位数为 17.4%，范围在 4.6%至 52.CD3+CD4+CD26-T细胞之间的CD7变异系数小于100%，CD3+CD4+CD26-T细胞相对于CD3+CD8+T细胞的CD7荧光强度中位数为1.7，范围在0.4至3.5之间。在对照组中，每个实体在这些参数上都与 CHL 有一定程度的重叠。因此，我们构建了一个 "霍奇金评分"，以加强 CHL 与其他实体的区分。霍奇金评分阈值为 15.35 时，诊断 CHL 的灵敏度为 78.6%，特异度为 96.2%。将霍奇金评分纳入简单算法可将特异性提高到100%：在这项研究中，我们使用流式细胞术证明了 CHL 中 CD3+CD4+CD26- T 细胞的增加，并得出了诊断 CHL 的霍奇金评分。

{"title":"Implicit Flow Cytometric Diagnosis of Classic Hodgkin Lymphoma Using CD3+CD4+CD26− T-Cells","authors":"Curtis Gravenmier, Jinming Song, Haipeng Shao","doi":"10.1002/jcla.25096","DOIUrl":"10.1002/jcla.25096","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Flow cytometry is not routinely performed in clinical laboratories for the diagnosis of classic Hodgkin lymphoma (CHL).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Fourteen cases of CHL and 132 cases of the control group were studied by 10-color flow cytometry, with markers including CD3, CD4, CD7, CD8, and CD26, as well as calculated parameters such as the CD4:CD8 ratio, percent CD3<sup>+</sup>CD4<sup>+</sup>CD26<sup>−</sup> T-cells of CD3<sup>+</sup>CD4<sup>+</sup> T-cells, percent CD3<sup>+</sup>CD4<sup>+</sup>CD26<sup>−</sup> T-cells of total events, CD7 coefficient of variation among CD3<sup>+</sup>CD4<sup>+</sup>CD26<sup>−</sup> T-cells, and CD7 median fluorescence intensity of CD3<sup>+</sup>CD4<sup>+</sup>CD26<sup>−</sup> T-cells relative to CD3<sup>+</sup>CD8<sup>+</sup> T-cells.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>CHL cases showed a median percent CD3<sup>+</sup>CD4<sup>+</sup>CD26<sup>−</sup> of CD3<sup>+</sup>CD4<sup>+</sup> T-cells of 72.3% with range from 41.1% to 94.4%, median percent CD3<sup>+</sup>CD4<sup>+</sup>CD26<sup>−</sup> T-cells of total events of 17.4% with range from 4.6% to 52.5%, CD7 coefficient of variation among CD3<sup>+</sup>CD4<sup>+</sup>CD26<sup>−</sup> T-cells less than 100%, and CD7 median fluorescence intensity of CD3<sup>+</sup>CD4<sup>+</sup>CD26<sup>−</sup> T-cells relative to CD3<sup>+</sup>CD8<sup>+</sup> T-cells of 1.7 with range from 0.4 to 3.5. In the control group, every entity showed some degree of overlap with CHL in terms of these parameters. A “Hodgkin score” was thus constructed to enhance separation of CHL from other entities. A threshold Hodgkin score of 15.35 achieved a sensitivity of 78.6% and specificity of 96.2% in the diagnosis of CHL. Incorporating the Hodgkin score into a simple algorithm raises the specificity to 100%.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>In this study, we used flow cytometry to demonstrate increased CD3<sup>+</sup>CD4<sup>+</sup>CD26<sup>−</sup> T-cells in CHL, and derived a Hodgkin score for the diagnosis of CHL.</p>\u0000 </section>\u0000 </div>","PeriodicalId":15509,"journal":{"name":"Journal of Clinical Laboratory Analysis","volume":"38 17-18","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jcla.25096","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142132878","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

45S5 Bioactive Glass-Ointment Positively Effects on Wound Healing in Rats by Regulating TNFα, Il-10, VEGF, and TGFβ 45S5 生物活性玻璃软膏通过调节 TNFα、Il-10、VEGF 和 TGFβ 对大鼠伤口愈合产生积极影响

IF 2.6 4区医学 Q2 MEDICAL LABORATORY TECHNOLOGY

Journal of Clinical Laboratory Analysis

Pub Date : 2024-09-05 DOI: 10.1002/jcla.25094

Ömer Kırgız, Muhammed Enes Altuğ, Hüseyin Özkan, Mehmet Cengiz Han, Gökhan Akçakavak, Ali Can Özarslan, Sevil Yücel

Aim

This study aimed to investigate the effects of 45S5 bioactive glass-ointment (BG) on cutaneous wound healing in rats at the molecular, biochemical, and histopathological levels.

Materials and Methods

Thirty-two rats were divided into four groups (n = 8): Control, Sham, BG, and DEX (Dexpanthenol). While no wound treatment was applied to the CONTROL, a wound model was created in the Sham, and no treatment was applied. A wound model was created for other groups, and BG and DEX were applied locally for 21 days. During the 21-day experiment period, feed and water consumption and weight changes were observed. Wound areas were calculated on days 0, 3, 7, 4, and 21. Following treatment, the rats were euthanized and tissues from the wound area and blood samples were collected. While the expression levels of tumor necrosis factor-alpha (TNFα), Interleukin 6 (IL6), Interleukin 10 (IL10), transforming growth factor-beta (TGFβ), and vascular endothelial growth factor (VEGF) genes were determined by qPCR, the levels of TNFα, IL6, and IL10 proteins were measured by ELISA.

Results

It was observed that the BG group showed anti-inflammatory activity by suppressing TNFα levels and stimulating IL-10. In addition, it was determined that BG increased fibroblast activity and vascularization.

Conclusion

Current findings showed that topical application of BG has anti-inflammatory effects, while also accelerating healing by increasing vascularity and making positive contributions to tissue healing.

目的：本研究旨在从分子、生物化学和组织病理学水平研究 45S5 生物活性玻璃膏（BG）对大鼠皮肤伤口愈合的影响：将 32 只大鼠分为四组（n = 8）：材料：32 只大鼠被分为四组（n = 8）：对照组、虚弱组、BG 组和 DEX（右泛醇）组。对照组不进行任何伤口处理，而 Sham 组则创建了一个伤口模型，也不进行任何处理。为其他组创建伤口模型，并在局部施用 BG 和 DEX，持续 21 天。在 21 天的实验期间，观察饲料和水的消耗量以及体重变化。在第 0、3、7、4 和 21 天计算伤口面积。治疗后，对大鼠实施安乐死，并收集伤口部位的组织和血液样本。采用 qPCR 法测定肿瘤坏死因子-α（TNFα）、白细胞介素 6（IL6）、白细胞介素 10（IL10）、转化生长因子-β（TGFβ）和血管内皮生长因子（VEGF）基因的表达水平，采用 ELISA 法测定 TNFα、IL6 和 IL10 蛋白的水平：结果：观察发现，BG 组通过抑制 TNFα 水平和刺激 IL-10 显示出抗炎活性。此外，BG 还能增强成纤维细胞的活性和血管生成：目前的研究结果表明，局部应用生物碱具有抗炎作用，同时还能通过增加血管来加速愈合，并对组织愈合做出积极贡献。

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引用次数: 0

A Narrative Review of Bedaquiline and Delamanid: New Arsenals Against Multidrug-Resistant and Extensively Drug-Resistant Mycobacterium tuberculosis 贝达喹啉和德拉马尼的叙述性综述：对抗耐多药和广泛耐药结核分枝杆菌的新武器

IF 2.6 4区医学 Q2 MEDICAL LABORATORY TECHNOLOGY

Journal of Clinical Laboratory Analysis

Pub Date : 2024-09-04 DOI: 10.1002/jcla.25091

Nazanin Ahmad Khosravi, Mehrandokht Sirous, Azar Dokht Khosravi, Morteza Saki

Background

The treatment of multidrug-resistant (MDR-) and extensively drug-resistant tuberculosis (XDR-TB) is a formidable challenge. Treatment of MDR- and XDR-TB using bedaquiline (BDQ) and delamanid (DLM), two newly introduced medications, is steadily increasing. This narrative review aimed to present a concise overview of the existing information regarding BDQ and DLM, and elucidate their antimicrobial characteristics, resistance mechanisms, synergism with other drugs, and side effects.

Methods

To collect the required information about the antimicrobial properties, a search for scientific evidence from the Scopus, PubMed, and Embase databases was performed, and all recently published articles up to May 2024 were considered.

Results

BDQ had potent antimicrobial effects on various types of nontuberculous mycobacteria (NTM), including rapid-growing and slow-growing species, and MDR/XDR Mycobacterium tuberculosis. The mechanisms of BDQ resistance in M. tuberculosis primarily involve mutations in three genes: atpE, mmpR (Rv0678) and pepQ. BDQ may have synergistic effects when combined with DLM, pyrazinamide, and pretomanid/linezolid. BDQ has a low incidence of side effects. The use of BDQ may prolong the QTc interval. Similarly, DLM showed potent antimicrobial effects on NTM and MDR/XDR M. tuberculosis. The main resistance mechanisms to DLM are induced by mutations in fbiA, fbiB, fbiC, fgd1, and ddn genes. The DLM had synergistic effects with BDQ and moxifloxacin. The DLM also has few side effects in some patients including QTc prolongation.

Conclusion

BDQ and DLM are suitable antibiotics with few side effects for the treatment of MDR/XDR-TB. These antibiotics have synergistic effects when combined with other antituberculosis drugs.

背景耐多药（MDR-）和广泛耐药结核病（XDR-TB）的治疗是一项艰巨的挑战。使用贝达喹啉（BDQ）和地拉那米（DLM）这两种新引进的药物治疗 MDR- 和 XDR-TB 的病例正在稳步增加。本综述旨在简明扼要地概述有关贝达喹啉和地拉那米的现有信息，并阐明这两种药物的抗菌特性、耐药机制、与其他药物的协同作用以及副作用。方法为了收集所需的抗菌特性信息，从 Scopus、PubMed 和 Embase 数据库中搜索科学证据，并考虑了截至 2024 年 5 月的所有近期发表的文章。结果 BDQ 对各种类型的非结核分枝杆菌（NTM），包括快速生长和缓慢生长的菌种，以及 MDR/XDR 结核分枝杆菌具有强效抗菌作用。结核分枝杆菌对 BDQ 产生抗药性的机制主要涉及三个基因的突变：atpE、mmpR (Rv0678) 和 pepQ。BDQ与DLM、吡嗪酰胺和pretomanid/linezolid联用时可能会产生协同效应。BDQ 的副作用发生率较低。使用 BDQ 可能会延长 QTc 间期。同样，DLM 对 NTM 和 MDR/XDR 型结核杆菌也有很强的抗菌作用。对 DLM 的主要耐药机制是由 fbiA、fbiB、fbiC、fgd1 和 ddn 基因突变诱导的。DLM 与 BDQ 和莫西沙星具有协同作用。DLM 对一些患者的副作用也很小，包括 QTc 延长。结论 BDQ 和 DLM 是治疗 MDR/XDR-TB 的合适抗生素，副作用小。这些抗生素与其他抗结核药物合用时具有协同作用。

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引用次数: 0