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Oak gum mediated sustainable synthesis of gold nanoparticles (Au NPs): evaluation of its antioxidant and anti-colon cancer effects 橡树胶介导的金纳米粒子(Au-NPs)的可持续合成:其抗氧化和抗克隆癌症作用的评价
IF 2.8 4区 材料科学 Q2 CHEMISTRY, MULTIDISCIPLINARY Pub Date : 2022-05-27 DOI: 10.1080/17458080.2022.2050905
Lin Lu, Qing-ye Zhao, Zhihai Wang, Fang Ju
Abstract Herein, we represent the bio-synthesis of gold nanoparticles (Au NPs) employing Oak gum extract as the green template, an efficient natural and non-toxic reductant and stabilizer based on its phytochemicals. The characterization of as-synthesized Au NPs was performed through Fourier transformed infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), transmission electron microscopy (TEM), energy dispersive X-ray spectroscopy (EDS), elemental mapping, UV-Vis and X-ray diffraction (XRD). TEM analysis explored the average particle size being 10-15 nm. The as-developed material was biologically explored in the inhibition of human colon cancer. Cytotoxicity of assessed over standard colon cancer cell line HT-29 and Caco-2. Cell viability of the two cell lines showed excellent outcomes, as observed by MTT assay. Futhermore, the Au@O. Gum nanocomposite was employed in the determination of the antioxidant properties following DPPH radical scavenging potential in presence of butylated hydroxytoluene (BHT) as the positive control and the result was compared to the raw Oak gum which resulted the scavenging activity as 21.54 and 54.56% respectively. The obtained results evidently validate the Au@O. Gum material as a potent colon cancer protective drug for the treatment of human colon cancer.
摘要在此,我们介绍了以橡树胶提取物为绿色模板的金纳米粒子(Au NPs)的生物合成,橡树胶提取物是一种基于其植物化学物质的高效天然无毒还原剂和稳定剂。通过傅里叶变换红外光谱(FT-IR)、扫描电子显微镜(SEM)、透射电子显微镜(TEM)、能量色散X射线光谱(EDS)、元素图谱、UV-Vis和X射线衍射(XRD)对合成的Au NPs进行了表征。TEM分析发现平均粒径为10-15 nm。对as-开发的材料在抑制人类结肠癌癌症方面进行了生物学探索。评估的超过标准的结肠癌细胞系HT-29和Caco-2的细胞毒性。通过MTT分析观察到,两种细胞系的细胞活力显示出优异的结果。此外Au@O.以丁基羟基甲苯(BHT)为阳性对照,采用树胶纳米复合材料测定了DPPH自由基清除能力后的抗氧化性能,并与清除活性分别为21.54%和54.56%的生橡树胶进行了比较。所得结果明显验证了Au@O.树胶材料作为一种有效的癌症保护药物,用于治疗人类癌症。
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引用次数: 5
Construction of high-performance polymer hydrogel composite materials for artificial bionic organs 人造仿生器官高性能高分子水凝胶复合材料的构建
IF 2.8 4区 材料科学 Q2 CHEMISTRY, MULTIDISCIPLINARY Pub Date : 2022-05-19 DOI: 10.1080/17458080.2022.2073999
Ming Yu, Wenhao Liu, Huimin Zhang, Guanjun Liu, Feng Luo, D. Cao
Abstract Hydrogel, with excellent hydrophilicity biocompatibility and stimuli responsive, has been widely used in biomedical and artificial organs fields. However, synthetic hydrogel usually displays poor gel strength, poor toughness and slow speed of absorbing water. Therefore, extensive exploratory research is still required to develop new composite materials for the application of bionic viscera. In this work, we constructed a new kind of composite hydrogel by a facile wet chemistry method to cross-link three kinds of polymer resin, polyvinyl alcohol, carboxymethyl cellulose, and carboxymethyl starch. The conductivity, moisture content and elastic modulus of hydrogel can be controlled by the dosage of potassium salt, sodium salt, water and glycerol. After molding and testing, the electrical conductivity, elastic modulus, and moisture content of the artificial liver and kidney organs were similar to those of the corresponding pig viscera, which is superior to most of the current research on hydrogels. This work provides a facile approach to synthesize highly efficient hydrogel, which accelerates the application of hydrogel in bionic organs.
摘要水凝胶具有优异的亲水性、生物相容性和刺激反应性,在生物医学和人工器官领域得到了广泛的应用。但合成水凝胶凝胶强度差,韧性差,吸水速度慢。因此,开发用于仿生脏器的新型复合材料仍需进行广泛的探索性研究。本文采用易湿化学方法,将聚乙烯醇、羧甲基纤维素、羧甲基淀粉三种高分子树脂交联,构建了一种新型复合水凝胶。水凝胶的电导率、含水率和弹性模量可以通过钾盐、钠盐、水和甘油的用量来控制。经过成型和测试,人工肝肾器官的电导率、弹性模量和含水率与相应的猪内脏相似,优于目前大多数水凝胶研究。本研究为高效水凝胶的合成提供了一种简便的方法,加速了水凝胶在仿生器官中的应用。
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引用次数: 2
One-pot preparation of nano-scaled magnetic-pectin particles (Fe3O4@pectin NPs): cytotoxicity, antioxidant, and anti-liver cancer properties 一锅法制备纳米磁性果胶颗粒(Fe3O4@pectinNPs):细胞毒性、抗氧化和抗肝癌癌症特性
IF 2.8 4区 材料科学 Q2 CHEMISTRY, MULTIDISCIPLINARY Pub Date : 2022-05-19 DOI: 10.1080/17458080.2022.2063279
Qiaoyun Zhang, Wenyu Cui, Hong-Ye Guo, Baoqing Wang, He Wang, Jimei Zhang, Wenlan Li
Abstract This work described the one-pot synthesis of orange pectin encapsulated Fe3O4 nanoparticles (Fe3O4@Pectin NPs) which is prepared by co-precipitation of Fe(II/(III) ions in alkaline solution mediated by pectin. This process led to formation of magnetic nanoparticles within the network of pectin. Physicochemical characterization of the as-synthesized Fe3O4@Pectin NPs was carried out through Fourier transformed infrared spectroscopy (FT-IR), electron microscopy (SEM and TEM), energy dispersive X-ray spectroscopy (EDX), vibrating sample magnetometer (VSM) and X-ray diffraction (XRD). The in vitro cytotoxic and anti-liver cancer effects of biologically synthesized Fe3O4@Pectin NPs against pleomorphic hepatocellular carcinoma (SNU-387), hepatic ductal carcinoma (LMH/2A), morris hepatoma (McA-RH7777), and novikoff hepatoma (N1-S1 Fudr) cancer cell lines were assessed. The anti-liver cancer properties of the Fe3O4@Pectin NPs could significantly remove pleomorphic hepatocellular carcinoma (SNU-387), hepatic ductal carcinoma (LMH/2A), morris hepatoma (McA-RH7777), and novikoff hepatoma (N1-S1 Fudr) cancer cell lines in a time and concentration-dependent manner by MTT assay. The IC50 of the Fe3O4@Pectin NPs were 8, 13, 10, and 7 µg/mL against pleomorphic hepatocellular carcinoma (SNU-387), hepatic ductal carcinoma (LMH/2A), morris hepatoma (McA-RH7777), and novikoff hepatoma (N1-S1 Fudr) cancer cell lines. The antioxidant activity of Fe3O4@Pectin NPs was determined by DPPH method. The Fe3O4@Pectin NPs showed the high antioxidant activity according to the IC50 value. It seems that the anti-human liver cancer effect of recent nanoparticles is due to their antioxidant effects.
摘要本工作描述了一锅法合成橙色果胶包封的Fe3O4纳米粒子(Fe3O4@PectinNPs),其通过果胶介导的Fe(II/(III)离子在碱性溶液中的共沉淀而制备。这一过程导致在果胶网络中形成磁性纳米颗粒。合成物的理化性质Fe3O4@Pectin通过傅立叶变换红外光谱(FT-IR)、电子显微镜(SEM和TEM)、能量色散X射线光谱(EDX)、振动样品磁强计(VSM)和X射线衍射(XRD)进行了NPs。生物合成的体外细胞毒性和抗癌症作用Fe3O4@Pectin对多形性肝细胞癌(SNU-387)、肝导管癌(LMH/2A)、morris肝癌(McA-RH7777)和novikoff肝癌(N1-S1-Fudr)癌症细胞系的NP进行了评估。抗肝癌癌症的特性Fe3O4@PectinMTT法检测NPs对多形性肝细胞癌(SNU-387)、肝导管癌(LMH/2A)、morris肝癌(McA-RH7777)和novikoff肝癌(N1-S1-Fudr)癌症细胞株有明显的时间和浓度依赖性。的IC50Fe3O4@PectinNP分别为8、13、10和7 μg/mL对抗多形性肝细胞癌(SNU-387)、肝导管癌(LMH/2A)、莫里斯肝癌(McA-RH7777)和诺维科夫肝癌(N1-S1-Fudr)癌症细胞系。抗氧化活性Fe3O4@PectinNPs采用DPPH法测定。这个Fe3O4@Pectin根据IC50值,NP显示出高抗氧化活性。最近纳米颗粒的抗人肝癌癌症作用似乎是由于它们的抗氧化作用。
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引用次数: 6
Pectin mediated green synthesis of CuO nanoparticles: evaluation of its cytotoxicity, antioxidant and anti-human cervical cancer properties 果胶介导的CuO纳米颗粒绿色合成:细胞毒性、抗氧化和抗人宫颈癌特性的评价
IF 2.8 4区 材料科学 Q2 CHEMISTRY, MULTIDISCIPLINARY Pub Date : 2022-05-04 DOI: 10.1080/17458080.2021.2013470
X. Liang, L. Shi, Run Zhang, Mingying Zhang
Abstract Regarding applicative, facile, green chemical research, a bio-inspired approach is being reported for the synthesis of CuO nanoparticles by pectin (PEC) as a natural reducing and stabilizing agentin alkaline medium without using any toxic reducing agent. The biosynthesized CuO NPs@Pectin were characterized by advanced physicochemical techniques like ultraviolet–visible (UV–Vis), Fourier Transformed Infrared spectroscopy (FTIR), Scanning Electron Microscopy (SEM), Transmission Electron Microscopy (TEM), Energy Dispersive X-ray spectroscopy (EDX) and X-ray Diffraction (XRD) study. It has been established that pectin-stabilized copper nanoparticles have a spherical shape with a mean diameter from approximately 10 nm. To survey the cytotoxicity and anti-human cervical cancer effects of CuO NPs@Pectin, MTT assay was used on C-33 A [c-33a], SiHa, Ca Ski, DoTc2 4510, HT-3 and LM-MEL-41 cell lines. The resulting IC50 values of the CuO NPs@Pectin against C-33 A [c-33a], SiHa, Ca Ski, DoTc2 4510, HT-3 and LM-MEL-41 cell lines were 231, 214, 243, 376, 315 and 169 µg/mL, respectively. Antioxidant properties of CuO NPs@Pectin were surveyed through the DPPH test in presence of butylated hydroxytoluene as the positive control. The CuO NPs@Pectin inhibited half of the DPPH molecules in the concentration of 98 µg/mL. The cell viability of the treated cell lines was found to reduce dose-dependently over the CuO NPs@Pectin nanocomposite. After approving the above results in the clinical trial studies, the CuO NPs@Pectin can be used as a chemotherapeutic drug for the treatment of several types of cervical cancers.
摘要:从应用、简便、绿色的化学研究角度出发,本文报道了一种以果胶(PEC)为天然还原剂,在碱性介质中不使用任何有毒还原剂合成CuO纳米粒子的仿生方法。采用先进的物理化学技术,如紫外-可见(UV-Vis)、傅里叶变换红外光谱(FTIR)、扫描电子显微镜(SEM)、透射电子显微镜(TEM)、能量色散x射线能谱(EDX)和x射线衍射(XRD)研究对生物合成的CuO NPs@Pectin进行了表征。已经确定果胶稳定的铜纳米颗粒具有平均直径约为10 nm的球形。为了研究CuO NPs@Pectin对c-33a [c-33a]、SiHa、Ca Ski、DoTc2 4510、HT-3和LM-MEL-41细胞系的细胞毒性和抗人宫颈癌作用。CuO NPs@Pectin对c-33a [c-33a]、SiHa、Ca Ski、DoTc2 4510、HT-3和LM-MEL-41细胞株的IC50值分别为231、214、243、376、315和169µg/mL。以丁基羟基甲苯为阳性对照,通过DPPH试验考察了CuO NPs@Pectin的抗氧化性能。在浓度为98µg/mL时,CuO NPs@Pectin抑制了一半的DPPH分子。发现处理细胞系的细胞活力在CuO NPs@Pectin纳米复合材料上呈剂量依赖性降低。在临床试验研究中批准上述结果后,CuO NPs@Pectin可作为化疗药物用于治疗几种类型的宫颈癌。
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引用次数: 3
New experimental low-cost nanoscience technology for formulation of silver nanoparticles-activated carbon composite as a promising antiviral, biocide, and efficient catalyst 新的实验性低成本纳米科学技术,用于配制银纳米颗粒-活性炭复合材料,作为一种有前途的抗病毒、杀生物剂和高效催化剂
IF 2.8 4区 材料科学 Q2 CHEMISTRY, MULTIDISCIPLINARY Pub Date : 2022-04-29 DOI: 10.1080/17458080.2022.2068794
H. Fetouh, H. Abd-Elnaby, M. Alsubaie, E. R. Sallam
Abstract A simple low-cost one-pot photodeposition synthesis with no hazardous reactants or products is used to make silver nanoparticles-activated carbon composite (SNPs@AC). The SNPs are homogenously and photodeposited and absorbed into the activated carbon matrix. Both SNPs and SNPs@AC composite have particle sizes around 10 nm and 100 nm, respectively. The SNPs@AC composite showed good antiviral activity to VERO (ATCC ccl-81) cells. Zeta potential of SNPs@AC composite is −25 mV, showing that this colloidal system is electrically stable and resistant to coagulation. For many Gram-positive and Gram-negative bacteria, the SNPs@AC composite demonstrated strong antibacterial efficacy. The SNPs@AC composite has 75.72 percent anti-inflammatory effect at concentration 500 µg/mL. This composite has a maximum non-toxic concentration (MNTC) of 78.125 g/mL, which corresponds to antiviral activity of up to 96.7 percent against hepatitis A. virus (HAV). It is suggested as a candidate for pharmaceutical formulations, such as integration into the manufacture of N95 masks for COVID-19 infection protection. Concentration 160 μg/mL SNPs@AC composite has antioxidant activity 42.74% percent. The SNPs@AC composite exhibited selective catalytic activity for the organosynthesis hydrazination reaction of 4-chloro-3, 5-di-nitro-benzo-triflouride, giving 1-hydroxy-4-nitro-6-trifluoro-methyl benzotriazole, a common antiviral drug for severe acute respiratory syndrome (SARS). SNPs@composite's well-defined pores provide suitable active sites for binding reactants: 4-Cl-3, 5-di-NO2-benzotriflouride, and hydrazine, which react to create 1-hydroxy-4-nitro-6-trifluoromethyl benzotriazole, which diffuses into solution away from the catalyst surface, leaving the catalyst surface unaffected.
摘要采用一种简单、低成本、无危险反应物或产物的一锅光沉积合成方法制备银纳米颗粒活性炭复合材料(SNPs@AC)。SNPs被均匀地光沉积并吸收到活性炭基质中。SNPs和SNPs@AC复合材料的颗粒尺寸约为10 nm和100 nm。这个SNPs@AC该复合物对VERO(ATCC ccl-81)细胞具有良好的抗病毒活性。齐塔电位SNPs@AC复合值为-25 mV,表明该胶体系统是电稳定的并且耐凝结。对于许多革兰氏阳性菌和革兰氏阴性菌SNPs@AC该复合材料具有很强的抗菌作用。这个SNPs@AC复合物在浓度为500时具有75.72%的抗炎作用 µg/mL。该复合材料的最大无毒浓度(MNTC)为78.125 g/mL,相当于对甲型肝炎病毒(HAV)高达96.7%的抗病毒活性。它被建议作为药物配方的候选,例如整合到N95口罩的生产中,以保护新冠肺炎感染。浓度160 μg/mLSNPs@AC复合材料的抗氧化活性为42.74%。这个SNPs@AC该复合物对4-氯-3,5-二硝基-苯并三氟脲的有机合成肼化反应具有选择性催化活性,得到了治疗严重急性呼吸综合征(SARS)的常用抗病毒药物1-羟基-4-硝基-6-三氟-甲基苯并三唑。SNPs@composite定义明确的孔为结合反应物提供了合适的活性位点:4-Cl-3,5-二-NO2-苯并三氟脲和肼,它们反应生成1-羟基-4-硝基-6-三氟甲基苯并三唑,其扩散到远离催化剂表面的溶液中,使催化剂表面不受影响。
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引用次数: 4
Design and synthesis of copper nanoparticles for the treatment of human esophageal cancer: introducing a novel chemotherapeutic supplement 设计和合成用于治疗人类食管癌的铜纳米颗粒:介绍一种新的化疗补充剂
IF 2.8 4区 材料科学 Q2 CHEMISTRY, MULTIDISCIPLINARY Pub Date : 2022-04-22 DOI: 10.1080/17458080.2022.2065264
Xiaofei Zhuang, Y. Kang, Lingxia Zhao, Shiping Guo
Abstract In this study, copper nanoparticles (Cu NPs) were synthesized in an eco-friendly pathway applying Mentha piperita extract as reducing/stabilizing agent. The morphological and physicochemical features of the prepared nanoparticles were determined using several advanced techniques like FE-SEM, EDX, and UV–Vis studies. The as synthesized Cu NPs was explored in the antioxidant and anti-human esophageal cancer tests. The in vitro cytotoxic and anti-esophageal cancer effects of biologically synthesized Cu NPs against human esophageal squamous cell carcinoma (KYSE-270), human caucasian esophageal carcinoma (OE33), and adenocarcinoma of the gastroesophageal junction (ESO26) cancer cell lines were assessed. The anti-esophageal cancer properties of the Cu NPs could significantly remove KYSE-270, OE33, and ESO26 cancer cell lines in a time and concentration-dependent manner by MTT assay. The IC50 of the Cu NPs were 241, 278, and 240 µg/mL against KYSE-270, OE33, and ESO26 cancer cell lines. The antioxidant activity of Cu NPs was determined by DPPH method. The Cu NPs showed the high antioxidant activity according to the IC50 value. It seems that the anti-human esophageal cancer effect of recent nanoparticles is due to their antioxidant effects.
摘要本研究以薄荷提取物为还原剂/稳定剂,采用生态友好的方法合成了铜纳米粒子。利用FE-SEM、EDX和UV-Vis等先进技术对制备的纳米颗粒的形态和物理化学特征进行了测定。对合成的铜NPs进行了抗氧化和抗人食管癌试验。研究了生物合成Cu NPs对人食管鳞状细胞癌(KYSE-270)、人高加索食管癌(OE33)和胃食管交界处腺癌(ESO26)细胞系的体外细胞毒和抗食管癌作用。MTT实验显示,Cu NPs对KYSE-270、OE33和ESO26癌细胞的抑制作用呈时间和浓度依赖性。Cu NPs对KYSE-270、OE33和ESO26癌细胞的IC50分别为241、278和240µg/mL。采用DPPH法测定铜NPs的抗氧化活性。IC50值表明,铜NPs具有较高的抗氧化活性。纳米颗粒的抗食管癌作用似乎是由于其抗氧化作用。
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引用次数: 8
Anti-human lung adenocarcinoma, cytotoxicity, and antioxidant potentials of copper nanoparticles green-synthesized by Calendula officinalis 金盏菊合成纳米铜绿的抗人肺腺癌、细胞毒性和抗氧化潜能
IF 2.8 4区 材料科学 Q2 CHEMISTRY, MULTIDISCIPLINARY Pub Date : 2022-04-22 DOI: 10.1080/17458080.2022.2066082
J. Gu, A. Aidy, S. Goorani
Abstract Calendula officinalis is known as a popular plant with various uses as pharmaceutical agent or food additive around the world. In this study, cooper nanoparticles were green synthesized using the aqueous extract of Calendula officinalis as the stabilizing, reducing, and capping agents. The formation of CuNPs@C. officinalis was screened using different chemical technique such as FT-IR spectroscopy, XRD, SEM, and Energy EDS. The antioxidant and anti-cancer activity of CuNPs@C. officinalis was studied using common assay including free radical scavenging and MTT assays. The results confirm the green synthesized of CuNPs@C. officinalis with aspherical morphology in the range size of 19.64 to 39.15 nm. In the antioxidant test, the IC50 of CuNPs@C. officinalis and BHT against DPPH free radicals were 193 and 154 µg/mL, respectively. In the cellular and molecular part of the recent study, the treated cells with CuNPs@C. officinalis were assessed by MTT assay for 24, 48, and 72 h about the cytotoxicity and anti-human lung adenocarcinoma properties on normal (HUVEC) and lung adenocarcinoma cell lines, i.e. lung moderately differentiated adenocarcinoma (LC-2/ad), lung poorly differentiated adenocarcinoma (PC-14), and lung well-differentiated bronchogenic adenocarcinoma (HLC-1). The IC50s of CuNPs@C. officinalis were 297, 328, and 514 µg/mL against PC-14, LC-2/ad, and HLC-1 cell lines, respectively. The viability of malignant lung cell line reduced dose-dependently in the presence of CuNPs@C. officinalis. It seems that the anti-human lung adenocarcinoma effect of recent nanoparticles is due to their antioxidant effects.
摘要金盏花是一种广受欢迎的植物,在世界各地具有多种药用或食品添加剂的用途。本研究以金盏花水提取物为稳定剂、还原剂和封端剂,绿色合成了铜纳米粒子。形成CuNPs@C.采用红外光谱、X射线衍射、扫描电镜、能量能谱等多种化学方法对山药材进行了筛选CuNPs@C.采用常用的自由基清除法和MTT法对厚朴进行了研究。结果证实了合成的绿色CuNPs@C.非球面形态的厚朴,尺寸在19.64至39.15之间 nm。在抗氧化剂测试中CuNPs@C.officinalis和BHT对DPPH自由基的抑制作用分别为193和154 µg/mL。在最近研究的细胞和分子部分CuNPs@C.通过MTT法评估24、48和72 h关于正常(HUVEC)和肺腺癌细胞系的细胞毒性和抗人肺腺癌特性,即肺中分化腺癌(LC-2/ad)、肺低分化腺腺癌(PC-14)和肺高分化支气管腺癌(HLC-1)。的IC50CuNPs@C.officinalis分别为297、328和514 µg/mL分别对抗PC-14、LC-2/ad和HLC-1细胞系。恶性肺细胞系的生存能力在存在CuNPs@C.厚朴。最近纳米颗粒的抗人肺腺癌作用似乎是由于它们的抗氧化作用。
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引用次数: 13
Synthesis, crystal structure, hirshfeld surface analysis, molecular docking and molecular dynamics studies of novel olanzapinium 2,5-dihydroxybenzoate as potential and active antipsychotic compound 新型抗精神病活性化合物2,5-二羟基苯甲酸奥氮平的合成、晶体结构、hirshfeld表面分析、分子对接和分子动力学研究
IF 2.8 4区 材料科学 Q2 CHEMISTRY, MULTIDISCIPLINARY Pub Date : 2022-04-15 DOI: 10.1080/17458080.2022.2063278
V. Natchimuthu, Mohnad Abdalla, Manasi Yadav, Ishita Chopra, Anushka Bhrdwaj, K. Sharma, S. Ravi, K. Ravikumar, K. Alzahrani, T. Hussain, Anuraj Nayarisseri
Abstract The antipsychotic drug Olanzapine was crystallized with aromatic acid, 2,5-dihydroxybenzoic acid in isopropyl alcohol by slow evaporation which led to the formation of olanzapinium 2,5-dihydroxybenzoate crystalline salt. The structure of the compound was characterized by 1H-NMR, 13C-NMR, and single-crystal X-ray diffraction analysis. The Hirshfeld analyses were performed to quantify the order and nature of intermolecular interactions in the crystal network. Employing computational approaches, the compound was tested for its affinity against antipsychotic activity by molecular docking and molecular dynamic simulation to attest the conformational stability over time step of 100 ns. Besides, bioactivity and ADMET properties were also predicted to ratify the result. The compound asserted neither carcinogenic nor mutagenic activity and has high oral bioavailability. Hence, this synthesized novel compound Olanzapinium 2,5-dihydroxybenzoate recognized in the study possesses high potential as an effective antipsychotic compound, and can further be examined for its efficiency by in vivo studies. The synthesized compound was submitted to NCBI PubChem database using accession substance ID: 441329256. The crystal structure was submitted to CCDC (Cambridge Crystallographic Data Centre) with submission ID: 2010899.
摘要将抗精神病药物奥氮平与芳香酸2,5-二羟基苯甲酸在异丙醇中缓慢蒸发结晶,得到奥氮平2,5-二羟基苯甲酸结晶盐。通过1H-NMR、13C-NMR和单晶x射线衍射对化合物的结构进行了表征。Hirshfeld分析是为了量化晶体网络中分子间相互作用的顺序和性质。采用计算方法,通过分子对接和分子动力学模拟测试了化合物的抗精神病活性,以验证其在100 ns时间步长的构象稳定性。此外,还进行了生物活性和ADMET特性的预测,验证了结果。该化合物既不致癌也不致突变,具有较高的口服生物利用度。因此,本研究所合成的新化合物奥氮平2,5-二羟基苯甲酸酯作为一种有效的抗精神病药物具有很高的潜力,并且可以通过体内研究进一步验证其有效性。合成的化合物提交到NCBI PubChem数据库,登录物质ID: 441329256。晶体结构提交给CCDC(剑桥晶体数据中心),提交ID: 2010899。
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引用次数: 0
Genotoxicity and in vitro investigation of Gefitinib-loaded polycaprolactone fabricated nanoparticles for anticancer activity against NCI-H460 cell lines 吉非替尼聚己内酯纳米颗粒抗NCI-H460细胞的遗传毒性及体外研究
IF 2.8 4区 材料科学 Q2 CHEMISTRY, MULTIDISCIPLINARY Pub Date : 2022-04-12 DOI: 10.1080/17458080.2022.2060501
Sankha Bhattacharya
Abstract For non-small cell lung cancer (NSCLC) treatment, a BCS class II drug, Gefitinib, was widely used. Due to poor bioavailability, uncontrollable drug release, Gefitinib witnessed side effects. To circumvent such associated problems, optimized Gefitinib encapsulated polycaprolactone (PCL) nanoparticles with three different molecular weights of PCL (average Mn∼10,000, Mn∼45,000 & Mn∼80,000) were developed using Box–Behnken design while understanding the influence of critical process parameters of the nanoparticles. For morphological characterizations, SEM, TEM, AFM were used. Hemocompatibility, platelet aggregation, and erythrocyte membrane integrity tests were used to test nanoparticles for biocompatibility; excellent biocompatibility was reported during these tests. The in-vitro drug release studies confirmed that Gefitinib-PCL10,000NPs, Gefitinib-PCL45,000NPs, and Gefitinib-PCL80,000 NPs, show significant initial burst effects, and later nanoparticles possessed zero-order kinetics. The genotoxicity of PCL nanoparticles was assessed by cytokinesis-block micronucleus (CBMN) assay, indicating DNA damage in NCI-H460 cell and micronuclei and nuclear buds’ formation. Further, reactive oxygen species studies, MTT cytotoxicity assays at 24 & 48 h, stability, in-vitro cellular uptake of optimized fluorescent Gefitinib PCL80,000NPs, and apoptosis studies were also carried out. As a result, investigating stable Gefitinib-loaded poly-caprolactone (PCL) nanoparticles could open up new research avenues, potentially lowering side effects and improving Gefitinib's profile in the treatment of NSCLC.
摘要对于癌症(NSCLC)的治疗,BCS II类药物吉非替尼被广泛使用。由于生物利用度差,药物释放不可控,吉非替尼出现了副作用。为了避免这些相关问题,使用Box-Behnken设计开发了具有三种不同分子量PCL(平均Mn~10000、Mn~45000和Mn~80000)的优化吉非替尼包封聚己内酯(PCL)纳米颗粒,同时了解了纳米颗粒的关键工艺参数的影响。使用SEM、TEM、AFM进行形态表征。血液相容性、血小板聚集和红细胞膜完整性测试用于测试纳米颗粒的生物相容性;在这些试验中报告了良好的生物相容性。体外药物释放研究证实,吉非替尼-PCL10000NP、吉非替尼布-PCL45000NP和吉非替尼·PCL80000 NP显示出显著的初始爆裂效应,随后的纳米颗粒具有零级动力学。通过胞质分裂阻断微核(CBMN)试验评估PCL纳米粒子的遗传毒性,表明NCI-H460细胞的DNA损伤以及微核和核芽的形成。此外,活性氧研究,24和48小时MTT细胞毒性测定 h、 还进行了稳定性、优化的荧光吉非替尼PCL80000NP的体外细胞摄取和细胞凋亡研究。因此,研究稳定的吉非替尼负载的聚己内酯(PCL)纳米颗粒可以开辟新的研究途径,有可能降低副作用,改善吉非替尼治疗NSCLC的效果。
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引用次数: 8
RETRACTED ARTICLE: Preparation of hollow Aux-Cu2O nanospheres by galvanic replacement to enhance the selective electrocatalytic CO2 reduction to ethanol 电取代法制备空心氧化铜纳米球,提高CO2电催化还原乙醇的选择性
IF 2.8 4区 材料科学 Q2 CHEMISTRY, MULTIDISCIPLINARY Pub Date : 2022-04-08 DOI: 10.1080/17458080.2021.2005246
Li-Jie Zhang, Ying Zhang, Hongtao Wang, Jianbing Chen, Zhongqi Cao
Abstract Electrocatalytic CO2 reduction to fuel is one of the important ways to solve energy and environmental problems. In this work, the preparation of hollow Aux-Cu2O electrocatalyst and the performance of electrocatalytic CO2 reduction to ethanol were studied. Hollow Cu2O nanospheres were prepared by a soft template method, and Aux-Cu2O composites were prepared by galvanic replacement. The characterization results of XRD and XPS reveal that Cu+ is the main chemical state of Cu in the catalysts. The results of electroactive surface area demonstrate that the electroactive surface area of Au0.51-Cu2O is the largest. The performance evaluation of electrocatalytic CO2 reduction shows that the Faraday efficiency of H2 on Au0.51-Cu2O is the lowest (∼19.5%) and the Faraday efficiency of ethanol can reach ∼18.8% at −1.2 V vs. RHE. Compared with hollow Cu2O nanospheres, Aux-Cu2O catalysts have an earlier onset for ethanol production and promote the CO2 reduction to ethanol with high efficiency, while the hydrogen evolution reaction is significantly inhibited. Our study demonstrates an effective approach to develop Cu-based electrocatalysts favourable toward ethanol in electrocatalytic CO2 reduction.
摘要电催化CO2还原为燃料是解决能源和环境问题的重要途径之一。本文研究了中空Aux-Cu2O电催化剂的制备及其电催化CO2还原为乙醇的性能。采用软模板法制备了中空Cu2O纳米球,并用电流置换法制备了Aux-Cu2O复合材料。XRD和XPS表征结果表明,Cu+是催化剂中Cu的主要化学态。电活性表面积的测定结果表明,Au0.51-Cu2O的电活性比表面积最大。电催化CO2还原的性能评估表明,H2在Au0.51-Cu2O上的法拉第效率最低(~19.5%),乙醇的法拉第效率在−1.2时可达~18.8% V与RHE。与中空Cu2O纳米球相比,Aux-Cu2O催化剂对乙醇生产的起始时间更早,并高效促进CO2还原为乙醇,同时显著抑制析氢反应。我们的研究证明了开发有利于乙醇电催化CO2还原的铜基电催化剂的有效方法。
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引用次数: 4
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Journal of Experimental Nanoscience
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