Pub Date : 2022-09-08DOI: 10.1080/17458080.2022.2120194
Zhenyu Zhao, Gang Liu, Yi-Chi Lin, Ling-xi Wang
Abstract In the present study, zinc oxide nanoparticles were green-synthesized using the aqueous extract of the rhizomes of Cyperus rotundus. The chemical methods EDX, FE-SEM, XRD, UV-Vis., and FT-IR analysis were used to characterize ZnONPs@C. rotundus were characterized by analytical techniques including. The FE-SEM image revealed a spherical shape for the nanoparticles in a size range of 38.05 to 75.41 nm and 33.09 nm was calculated for ZnONPs@ C. rotundus crystal size using the XRD results. MTT assay was used on common lung well-differentiated bronchogenic adenocarcinoma cell line i.e. HLC-1 to survey the cytotoxicity and anti-lung well-differentiated bronchogenic adenocarcinoma effects of the synthesized nanoparticles. To determine the antioxidant properties of the synthesized nanoparticles, the DPPH test was used in the presence of butylated hydroxytoluene as the positive control. The synthesized nanoparticles had very low cell viability and high anti-lung well-differentiated bronchogenic adenocarcinoma activities dose-dependently against the HLC-1 cell line without any cytotoxicity on the normal cell line (HUVEC). The synthesized nanoparticles inhibited half of the DPPH molecules at the concentration of 41 µg/mL. Maybe significant anti-human lung well-differentiated bronchogenic adenocarcinoma potentials of the synthesized nanoparticles against common human lung well-differentiated bronchogenic adenocarcinoma cell lines are linked to their antioxidant activities.
{"title":"Green chemistry approach to the synthesis of zinc nanoparticles using Cyperus rotundus rhizome extract for the treatment of lung well-differentiated bronchogenic adenocarcinoma","authors":"Zhenyu Zhao, Gang Liu, Yi-Chi Lin, Ling-xi Wang","doi":"10.1080/17458080.2022.2120194","DOIUrl":"https://doi.org/10.1080/17458080.2022.2120194","url":null,"abstract":"Abstract In the present study, zinc oxide nanoparticles were green-synthesized using the aqueous extract of the rhizomes of Cyperus rotundus. The chemical methods EDX, FE-SEM, XRD, UV-Vis., and FT-IR analysis were used to characterize ZnONPs@C. rotundus were characterized by analytical techniques including. The FE-SEM image revealed a spherical shape for the nanoparticles in a size range of 38.05 to 75.41 nm and 33.09 nm was calculated for ZnONPs@ C. rotundus crystal size using the XRD results. MTT assay was used on common lung well-differentiated bronchogenic adenocarcinoma cell line i.e. HLC-1 to survey the cytotoxicity and anti-lung well-differentiated bronchogenic adenocarcinoma effects of the synthesized nanoparticles. To determine the antioxidant properties of the synthesized nanoparticles, the DPPH test was used in the presence of butylated hydroxytoluene as the positive control. The synthesized nanoparticles had very low cell viability and high anti-lung well-differentiated bronchogenic adenocarcinoma activities dose-dependently against the HLC-1 cell line without any cytotoxicity on the normal cell line (HUVEC). The synthesized nanoparticles inhibited half of the DPPH molecules at the concentration of 41 µg/mL. Maybe significant anti-human lung well-differentiated bronchogenic adenocarcinoma potentials of the synthesized nanoparticles against common human lung well-differentiated bronchogenic adenocarcinoma cell lines are linked to their antioxidant activities.","PeriodicalId":15673,"journal":{"name":"Journal of Experimental Nanoscience","volume":"17 1","pages":"535 - 547"},"PeriodicalIF":2.8,"publicationDate":"2022-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47919364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-09-05DOI: 10.1080/17458080.2022.2112919
M. Waqas, Shees Safdar, M. Buabeid, A. Ashames, M. Akhtar, Ghulam Murtaza
Abstract Chitosan-based nano-sized particles increase the penetration of the drug through the narrow junction into the bloodstream and target the specific site. The objective of this study was to prepare chitosan nanoparticles to entrap a hydrophobic drug (tamoxifen citrate), followed by the alginate coating of the developed nanoparticles to decrease their degradation in the acidic pH. Drug-loaded chitosan nanoparticles were prepared by the ionic gelation method. Alginate coating was done by dissolving sodium alginate to buffer solution and drug-loaded chitosan nanoparticles drop-wise under mild agitation. The size of alginate coated chitosan nanoparticles, zeta potential, surface morphology, in-vitro drug release, and entrapment efficiency was measured. The optimised formulation of both uncoated (SH3) and coated (SH7) formulation showed the particle size, PDI, and zeta potential with values 221 & 338 nm, 0.161 & 0.230 and 36.5 & −20.7 mV, respectively. The resulted nanoparticle surface was non-porous. The percentage yield of the optimised formulation SH3 was 28% and SH6 was 33%. The entrapment efficiency of the optimised formulation SH3 (uncoated formulation) and SH6 (coated formulation) is 69.5 and 58.51%, respectively. Chitosan nanoparticles were successfully prepared to entrap tamoxifen citrate. The coating of chitosan nanoparticles decreased their degradation in the acidic pH.
{"title":"Alginate-coated chitosan nanoparticles for pH-dependent release of tamoxifen citrate","authors":"M. Waqas, Shees Safdar, M. Buabeid, A. Ashames, M. Akhtar, Ghulam Murtaza","doi":"10.1080/17458080.2022.2112919","DOIUrl":"https://doi.org/10.1080/17458080.2022.2112919","url":null,"abstract":"Abstract Chitosan-based nano-sized particles increase the penetration of the drug through the narrow junction into the bloodstream and target the specific site. The objective of this study was to prepare chitosan nanoparticles to entrap a hydrophobic drug (tamoxifen citrate), followed by the alginate coating of the developed nanoparticles to decrease their degradation in the acidic pH. Drug-loaded chitosan nanoparticles were prepared by the ionic gelation method. Alginate coating was done by dissolving sodium alginate to buffer solution and drug-loaded chitosan nanoparticles drop-wise under mild agitation. The size of alginate coated chitosan nanoparticles, zeta potential, surface morphology, in-vitro drug release, and entrapment efficiency was measured. The optimised formulation of both uncoated (SH3) and coated (SH7) formulation showed the particle size, PDI, and zeta potential with values 221 & 338 nm, 0.161 & 0.230 and 36.5 & −20.7 mV, respectively. The resulted nanoparticle surface was non-porous. The percentage yield of the optimised formulation SH3 was 28% and SH6 was 33%. The entrapment efficiency of the optimised formulation SH3 (uncoated formulation) and SH6 (coated formulation) is 69.5 and 58.51%, respectively. Chitosan nanoparticles were successfully prepared to entrap tamoxifen citrate. The coating of chitosan nanoparticles decreased their degradation in the acidic pH.","PeriodicalId":15673,"journal":{"name":"Journal of Experimental Nanoscience","volume":"17 1","pages":"522 - 534"},"PeriodicalIF":2.8,"publicationDate":"2022-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43395984","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-09-01DOI: 10.1080/17458080.2022.2115483
Anju Mishra, Archana Singh, Hemant R Kushwaha, A. Mishra
Abstract Cobalt oxide/graphene oxide (Co3O4/GO) nanocomposites were synthesised using the co-precipitation synthesis process. The polycrystalline nature of Co3O4 nanoparticles onto GO sheets is studied by X-ray diffraction (XRD) pattern where nanoparticles were found in polycrystalline nature with a particle size of 35 nm. The structural and morphological were using field emission scanning electron microscopy (FESEM) and EDX, where a distribution of Co3O4 nanoparticles on GO nanosheets was observed. The effect of Co3O4 nanoparticles on GO nanosheets was studied using a Raman spectrometer and found enhancement in the Raman peaks of GO sheets after the decoration of Co3O4 nanoparticles on GO nanosheets. It is observed that the ID/IG ratio of the D and G bands was increased from 1.08 (GO) to 1.11 (Co3O4/GO). In our present study, we explored the potential cytotoxic effects of Co3O4/GO nanocomposites in Mice melanoma cells (B16F10), where MTT assay suggested that (Co3O4/GO) nanocomposite shows a significant effect on cell viability compared to GO i.e. 60% cell viability was observed at 200 µg/mL of GO whereas it was only 37% for Co3O4/GO nanocomposite indicating improved anti-cancerous activity at this concentration. This is the first time that Co3O4/GO nanocomposite is tested for its cytotoxic effect and the results suggest that it can be used as an alternative source for tumour or cancer treatment.
{"title":"Cytotoxic effect of cobalt oxide–graphene oxide nanocomposites on melanoma cell line","authors":"Anju Mishra, Archana Singh, Hemant R Kushwaha, A. Mishra","doi":"10.1080/17458080.2022.2115483","DOIUrl":"https://doi.org/10.1080/17458080.2022.2115483","url":null,"abstract":"Abstract Cobalt oxide/graphene oxide (Co3O4/GO) nanocomposites were synthesised using the co-precipitation synthesis process. The polycrystalline nature of Co3O4 nanoparticles onto GO sheets is studied by X-ray diffraction (XRD) pattern where nanoparticles were found in polycrystalline nature with a particle size of 35 nm. The structural and morphological were using field emission scanning electron microscopy (FESEM) and EDX, where a distribution of Co3O4 nanoparticles on GO nanosheets was observed. The effect of Co3O4 nanoparticles on GO nanosheets was studied using a Raman spectrometer and found enhancement in the Raman peaks of GO sheets after the decoration of Co3O4 nanoparticles on GO nanosheets. It is observed that the ID/IG ratio of the D and G bands was increased from 1.08 (GO) to 1.11 (Co3O4/GO). In our present study, we explored the potential cytotoxic effects of Co3O4/GO nanocomposites in Mice melanoma cells (B16F10), where MTT assay suggested that (Co3O4/GO) nanocomposite shows a significant effect on cell viability compared to GO i.e. 60% cell viability was observed at 200 µg/mL of GO whereas it was only 37% for Co3O4/GO nanocomposite indicating improved anti-cancerous activity at this concentration. This is the first time that Co3O4/GO nanocomposite is tested for its cytotoxic effect and the results suggest that it can be used as an alternative source for tumour or cancer treatment.","PeriodicalId":15673,"journal":{"name":"Journal of Experimental Nanoscience","volume":"17 1","pages":"509 - 521"},"PeriodicalIF":2.8,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45785403","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-08-29DOI: 10.1080/17458080.2022.2110241
Ping Hou, Hongyi Kuang, Wei Deng, Yan Lei
Abstract In recent days, the green synthesized nanomagnetic biocomposites have been evolved with tremendous potential as the future catalysts. This has encouraged us to design and synthesis of a novel Cu NPs fabricated potato starch functionalized magnetic nanomaterial (Fe3O4@starch/Cu nanocomposite). The prepared nanocomposite were characterized using advanced analytical techniques like FT-IR, FESEM, TEM, EDX, elemental mapping and ICP-OES. The biogenic synthesized Fe3O4@starch/Cu nanocomposite was explored in the anti-ovarian cancer investigations against ovarian cancer cell lines (PA-1, Caov-3, SW 626, and SK-OV-3). The material exhibited significant cytotoxicities against the ovarian cancer cell lines. However, it was considerably inactive against the normal HUVEC cell line. The antioxidant potential of Fe3O4@starch/Cu nanocomposite was also investigated by DPPH method. The Fe3O4@starch/Cu nanocomposite revealed the significant antioxidant potentials according to the IC50 value.
{"title":"Immobilized copper nanoparticles on biodegradable magnetic starch composite: investigation of its ovarian cancer, cytotoxicity, and antioxidant effects","authors":"Ping Hou, Hongyi Kuang, Wei Deng, Yan Lei","doi":"10.1080/17458080.2022.2110241","DOIUrl":"https://doi.org/10.1080/17458080.2022.2110241","url":null,"abstract":"Abstract In recent days, the green synthesized nanomagnetic biocomposites have been evolved with tremendous potential as the future catalysts. This has encouraged us to design and synthesis of a novel Cu NPs fabricated potato starch functionalized magnetic nanomaterial (Fe3O4@starch/Cu nanocomposite). The prepared nanocomposite were characterized using advanced analytical techniques like FT-IR, FESEM, TEM, EDX, elemental mapping and ICP-OES. The biogenic synthesized Fe3O4@starch/Cu nanocomposite was explored in the anti-ovarian cancer investigations against ovarian cancer cell lines (PA-1, Caov-3, SW 626, and SK-OV-3). The material exhibited significant cytotoxicities against the ovarian cancer cell lines. However, it was considerably inactive against the normal HUVEC cell line. The antioxidant potential of Fe3O4@starch/Cu nanocomposite was also investigated by DPPH method. The Fe3O4@starch/Cu nanocomposite revealed the significant antioxidant potentials according to the IC50 value.","PeriodicalId":15673,"journal":{"name":"Journal of Experimental Nanoscience","volume":"17 1","pages":"496 - 508"},"PeriodicalIF":2.8,"publicationDate":"2022-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47941604","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-07-19DOI: 10.1080/17458080.2022.2094915
U. Afreen, Khairi Mustafa Salem Fahelelbom, Syed Nisar Hussain Shah, A. Ashames, U. Almas, S. Khan, M. Yameen, Naveed Nisar, M. Asad, G. Murtaza
Abstract Purpose of present study was to develop eight formulations of chlorpheniramine (CPM) niosomes according to 23 factorial design, characterise on the basis of various evaluation tests, i.e. in vitro drug release, SEM, FTIR, TGA and release kinetics, optimise the eight formulation on the basis in vitro drug release data, formulate gel of optimised dispersion, and to perform in vivo and histopathological study using gel of optimised dispersion on rabbits. Here, N3 having low level of cholesterol and span-80 but high level of span-60(0.1:0.2:0.05) was selected as optimised dispersion of niosomes that showed highest drug release i.e. 88.25% at pH 6 over 24 h of study and followed Korsmeyers-Peppas release kinetics with Fickian diffusion mechanism. After application of statistic by Analysis of variance (ANOVA) with 3D surface plots construction, gel of optimised dispersion of CPM niosomes was formulated, and evaluated by tests for i.e. viscosity, Spreadability, Extrudibility, drug content, drug entrapment, stability, SEM, FTIR, TGA, in vitro drug release, in vivo drug release following first order kinetics and histopathological study. Niosomal gel of CPM ensured successful development using suitable combination of non-ionic surfactants, and effective loading of drug for targeted delivery of drug.
{"title":"Formulation and evaluation of niosomes-based chlorpheniramine gel for the treatment of mild to moderate skin allergy","authors":"U. Afreen, Khairi Mustafa Salem Fahelelbom, Syed Nisar Hussain Shah, A. Ashames, U. Almas, S. Khan, M. Yameen, Naveed Nisar, M. Asad, G. Murtaza","doi":"10.1080/17458080.2022.2094915","DOIUrl":"https://doi.org/10.1080/17458080.2022.2094915","url":null,"abstract":"Abstract Purpose of present study was to develop eight formulations of chlorpheniramine (CPM) niosomes according to 23 factorial design, characterise on the basis of various evaluation tests, i.e. in vitro drug release, SEM, FTIR, TGA and release kinetics, optimise the eight formulation on the basis in vitro drug release data, formulate gel of optimised dispersion, and to perform in vivo and histopathological study using gel of optimised dispersion on rabbits. Here, N3 having low level of cholesterol and span-80 but high level of span-60(0.1:0.2:0.05) was selected as optimised dispersion of niosomes that showed highest drug release i.e. 88.25% at pH 6 over 24 h of study and followed Korsmeyers-Peppas release kinetics with Fickian diffusion mechanism. After application of statistic by Analysis of variance (ANOVA) with 3D surface plots construction, gel of optimised dispersion of CPM niosomes was formulated, and evaluated by tests for i.e. viscosity, Spreadability, Extrudibility, drug content, drug entrapment, stability, SEM, FTIR, TGA, in vitro drug release, in vivo drug release following first order kinetics and histopathological study. Niosomal gel of CPM ensured successful development using suitable combination of non-ionic surfactants, and effective loading of drug for targeted delivery of drug.","PeriodicalId":15673,"journal":{"name":"Journal of Experimental Nanoscience","volume":"17 1","pages":"467 - 495"},"PeriodicalIF":2.8,"publicationDate":"2022-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44821365","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-07-07DOI: 10.1080/17458080.2022.2087872
Meng Lin, Jiangnan Zhang, Jun Li, Dechun Zhang, Ting Mo
Abstract To establish a gold/silica hybrid, nanomaterials (Au/SiO2) were incorporated into a gelatin methacrylate/chitosan matrix. By using FESEM, compressive strength testing, and conductivity/resistance measurements on gelatin (G)/chitosan (C), G/C-Au@SiO2 hydrogels developed. Biocompatibility investigations on osteoblasts MG-63 cells were carried out to determine whether the cell was compatible with the conductive hydrogel as it had been created. The results indicated that HNPs had improved compressive strength and conductivity without losing the favourable features such as biodegradable nature and porous shape of G/C hydrogel. The mechanical properties and Elastic modulus of composites hydrogels were enhanced twofold when hybrid nanomaterials were added to the mixture. The cyclic compressive analysis shows that pure G/C hydrogels lost their mechanical stability within the first few cycles, but G/C-Au@SiO2 hydrogels lasted for up to fifty cycles. It was demonstrated that osteoblast proliferation and adhesion were increased on the hydrogel in the CCK-8 experiment. Further, the cell survival of the hydrogels with G/C-Au@SiO2 conductivity was enhanced by 15% compared to that of pure G/C hydrogels. The morphological features of the MG-63 cells experiments were performed by using a Fluorescein diacetate hydrolysis (FDA) staining assay. This work offers a unique method for enhancing mechanical integrity and electrical properties in gelatin-based G/C hydrogels by adding bifunctional hybrid nanomaterials (HNPs) for bone fracture tissue engineering applications.
{"title":"A convergent synthetic platform of gold/silica nanomaterials functionalized gelatin/chitosan hydrogel framework for the bone fracture treatment","authors":"Meng Lin, Jiangnan Zhang, Jun Li, Dechun Zhang, Ting Mo","doi":"10.1080/17458080.2022.2087872","DOIUrl":"https://doi.org/10.1080/17458080.2022.2087872","url":null,"abstract":"Abstract To establish a gold/silica hybrid, nanomaterials (Au/SiO2) were incorporated into a gelatin methacrylate/chitosan matrix. By using FESEM, compressive strength testing, and conductivity/resistance measurements on gelatin (G)/chitosan (C), G/C-Au@SiO2 hydrogels developed. Biocompatibility investigations on osteoblasts MG-63 cells were carried out to determine whether the cell was compatible with the conductive hydrogel as it had been created. The results indicated that HNPs had improved compressive strength and conductivity without losing the favourable features such as biodegradable nature and porous shape of G/C hydrogel. The mechanical properties and Elastic modulus of composites hydrogels were enhanced twofold when hybrid nanomaterials were added to the mixture. The cyclic compressive analysis shows that pure G/C hydrogels lost their mechanical stability within the first few cycles, but G/C-Au@SiO2 hydrogels lasted for up to fifty cycles. It was demonstrated that osteoblast proliferation and adhesion were increased on the hydrogel in the CCK-8 experiment. Further, the cell survival of the hydrogels with G/C-Au@SiO2 conductivity was enhanced by 15% compared to that of pure G/C hydrogels. The morphological features of the MG-63 cells experiments were performed by using a Fluorescein diacetate hydrolysis (FDA) staining assay. This work offers a unique method for enhancing mechanical integrity and electrical properties in gelatin-based G/C hydrogels by adding bifunctional hybrid nanomaterials (HNPs) for bone fracture tissue engineering applications.","PeriodicalId":15673,"journal":{"name":"Journal of Experimental Nanoscience","volume":"17 1","pages":"451 - 465"},"PeriodicalIF":2.8,"publicationDate":"2022-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45709858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-06-25DOI: 10.1080/17458080.2022.2091132
Xiaomei Tian, Ju-xiao Lian, Xin Li, Xianxian Chen, Hongjun Yuan, Min Zhou, Fengyong Liu
Abstract Hepatocellular carcinoma (HCC) is associated with a high mortality rate. In this study, we aimed to investigate the therapeutic effect and safety of hepatic arterial administration of doxorubicin (Dox)-loaded hollow gold nanospheres (Dox@HAuNSs) combined with photothermal ablation (PTA) in a rabbit VX2 liver cancer model established by implanting VX2 tumour cells into rabbit livers. Rabbits were randomly divided into four treatment groups: physiological saline solution (control); lipiodol and doxorubicin (Dox + Lp); lipiodol and Dox@HAuNS (Dox@PEG-HAuNS + Lp); and lipiodol, Dox@PEG-HAuNS, and photothermal ablation (Dox@PEG-HAuNS + Lp + PTA). Dox release from Dox@PEG-HAuNS in the tumour was detected using fluorescence microscopy. Tumour size and liver-kidney function were assessed in each rabbit preoperatively and on postoperative days 3, 7, and 14. Necrosis, proliferation, and micro-vessel density of VX2 tumours were estimated in peripheral tumour tissues. Dox release from Dox@PEG-HAuNS was increased with a subsequent increase in tumour necrosis in liver tumours after PTA, whereas the tumour volume and proliferation decreased. However, the aspartate aminotransferase and alanine aminotransferase levels indicated transient liver damage. Thus, intra-arterial delivery of Dox@PEG-HAuNS combined with PTA can suppress VX2 liver cancer growth. Dox@PEG-HAuNS + Lp + PTA is also associated with transient liver damage in rabbits. The combination of Dox@PEG-HAuNS chemoembolisation and PTA could be a potential therapeutic approach for HCC and it has broad application prospects.
{"title":"Intra-arterial delivery of doxorubicin-loaded hollow gold nanospheres—photothermal ablation and chemoembolisation therapy in a rabbit VX2 liver cancer model","authors":"Xiaomei Tian, Ju-xiao Lian, Xin Li, Xianxian Chen, Hongjun Yuan, Min Zhou, Fengyong Liu","doi":"10.1080/17458080.2022.2091132","DOIUrl":"https://doi.org/10.1080/17458080.2022.2091132","url":null,"abstract":"Abstract Hepatocellular carcinoma (HCC) is associated with a high mortality rate. In this study, we aimed to investigate the therapeutic effect and safety of hepatic arterial administration of doxorubicin (Dox)-loaded hollow gold nanospheres (Dox@HAuNSs) combined with photothermal ablation (PTA) in a rabbit VX2 liver cancer model established by implanting VX2 tumour cells into rabbit livers. Rabbits were randomly divided into four treatment groups: physiological saline solution (control); lipiodol and doxorubicin (Dox + Lp); lipiodol and Dox@HAuNS (Dox@PEG-HAuNS + Lp); and lipiodol, Dox@PEG-HAuNS, and photothermal ablation (Dox@PEG-HAuNS + Lp + PTA). Dox release from Dox@PEG-HAuNS in the tumour was detected using fluorescence microscopy. Tumour size and liver-kidney function were assessed in each rabbit preoperatively and on postoperative days 3, 7, and 14. Necrosis, proliferation, and micro-vessel density of VX2 tumours were estimated in peripheral tumour tissues. Dox release from Dox@PEG-HAuNS was increased with a subsequent increase in tumour necrosis in liver tumours after PTA, whereas the tumour volume and proliferation decreased. However, the aspartate aminotransferase and alanine aminotransferase levels indicated transient liver damage. Thus, intra-arterial delivery of Dox@PEG-HAuNS combined with PTA can suppress VX2 liver cancer growth. Dox@PEG-HAuNS + Lp + PTA is also associated with transient liver damage in rabbits. The combination of Dox@PEG-HAuNS chemoembolisation and PTA could be a potential therapeutic approach for HCC and it has broad application prospects.","PeriodicalId":15673,"journal":{"name":"Journal of Experimental Nanoscience","volume":"17 1","pages":"439 - 450"},"PeriodicalIF":2.8,"publicationDate":"2022-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46768548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-06-10DOI: 10.1080/17458080.2022.2084533
Chuthapond Musimun, Dominika Papiernik, P. Permpoonpattana, P. Chumkaew, T. Srisawat
Abstract Silver nanoparticles (AgNPs) are used in biomedicine applications. Other drugs combined with the AgNPs can improve efficacy in the treatment of diseases, and most such studies have focused on antibiotics. We determined the synergistic effects of Phyllanthus emblica-derived AgNPs in combination with Vatica diospyroides cotyledon extracts (VCE) against bacteria using agar well diffusion, broth microdilution, and minimum inhibitory concentration (MIC). Synergy of AgNPs and VCE was confirmed with the fractional inhibitory concentration index (FICI). To evaluate patterns of bacterial death, flow cytometry and electron microscopy were used. We found that the effective incubation time of AgNPs against bacteria was highly variable. Increasing AgNPs in the combination influenced antibacterial activity against Staphylococcus aureus and Bacillus subtilis. The MIC values interpreted through FICI showed synergy against S. aureus and indifference against B. subtilis. Flow cytometric profiles confirmed that the fraction of S. aureus that respond to a combination of VCE with AgNPs increased in dose-dependent manner. The response patterns of bacteria proceeded simultaneously as the cells lost intracellular components and suffered membrane damage. Synergy of AgNPs with a plant extract has become a promising approach, as green AgNPs and plant extracts are biocompatible and cost-effective resources that can utilized for the treatment of bacterial infectious diseases.
{"title":"Synergy of green-synthesized silver nanoparticles and Vatica diospyroides fruit extract in inhibiting Gram-positive bacteria by inducing membrane and intracellular disruption","authors":"Chuthapond Musimun, Dominika Papiernik, P. Permpoonpattana, P. Chumkaew, T. Srisawat","doi":"10.1080/17458080.2022.2084533","DOIUrl":"https://doi.org/10.1080/17458080.2022.2084533","url":null,"abstract":"Abstract Silver nanoparticles (AgNPs) are used in biomedicine applications. Other drugs combined with the AgNPs can improve efficacy in the treatment of diseases, and most such studies have focused on antibiotics. We determined the synergistic effects of Phyllanthus emblica-derived AgNPs in combination with Vatica diospyroides cotyledon extracts (VCE) against bacteria using agar well diffusion, broth microdilution, and minimum inhibitory concentration (MIC). Synergy of AgNPs and VCE was confirmed with the fractional inhibitory concentration index (FICI). To evaluate patterns of bacterial death, flow cytometry and electron microscopy were used. We found that the effective incubation time of AgNPs against bacteria was highly variable. Increasing AgNPs in the combination influenced antibacterial activity against Staphylococcus aureus and Bacillus subtilis. The MIC values interpreted through FICI showed synergy against S. aureus and indifference against B. subtilis. Flow cytometric profiles confirmed that the fraction of S. aureus that respond to a combination of VCE with AgNPs increased in dose-dependent manner. The response patterns of bacteria proceeded simultaneously as the cells lost intracellular components and suffered membrane damage. Synergy of AgNPs with a plant extract has become a promising approach, as green AgNPs and plant extracts are biocompatible and cost-effective resources that can utilized for the treatment of bacterial infectious diseases.","PeriodicalId":15673,"journal":{"name":"Journal of Experimental Nanoscience","volume":"17 1","pages":"420 - 438"},"PeriodicalIF":2.8,"publicationDate":"2022-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44235121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-06-06DOI: 10.1080/17458080.2022.2080197
B. Alotaibi, A. Ashames, M. Buabeid, Momina Masood, S. Mir, G. Murtaza
Abstract Purpose: The aim of this study was to assess the antibacterial potential and ex vivo skin permeation kinetics of cefixime from bionanocomposite films. Methods: The films were prepared by solvent casting method by using chitosan and starch. The fabricated films were tested for their antibacterial potential against three bacteria i.e. Escherichia coli, Klebsiella pneumonia, and Acetobacter aceti. In vitro permeation studies of cefixime from the films across rat skin was conducted using Franz diffusion cell. Results: The highest antibacterial effect was exhibited by F5 formulation (non-irradiated film) against Escherichia coli and Klebsiella pneumonia; however, antibacterial activity of the films was significantly (p < 0.05) reduced after their irradiation. F5 formulation showed the highest cumulative amount of permeated drug after 24 h, while F1 (100% chitosan) showed the lowest amount of permeated drug. Non-Fickian diffusion (anomalous) was the main mode of drug release from all films. The cross-linking of films by γ-radiations improved their mechanical properties. The percentage swelling ratio was the highest in non-irradiated films having a polymeric blend (50:50). Water uptake of irradiated films was appreciably reduced as compared to non-irradiated films. Conclusion: The synthesized bionanocomposites are promising therapeutic moieties which not only improve drug permeability across but also ameliorates antibacterial potential of cefixime.
{"title":"A new approach for the management of Escherichia coli and Klebsiella pneumonia by using cefixime-based bionanocomposite films","authors":"B. Alotaibi, A. Ashames, M. Buabeid, Momina Masood, S. Mir, G. Murtaza","doi":"10.1080/17458080.2022.2080197","DOIUrl":"https://doi.org/10.1080/17458080.2022.2080197","url":null,"abstract":"Abstract Purpose: The aim of this study was to assess the antibacterial potential and ex vivo skin permeation kinetics of cefixime from bionanocomposite films. Methods: The films were prepared by solvent casting method by using chitosan and starch. The fabricated films were tested for their antibacterial potential against three bacteria i.e. Escherichia coli, Klebsiella pneumonia, and Acetobacter aceti. In vitro permeation studies of cefixime from the films across rat skin was conducted using Franz diffusion cell. Results: The highest antibacterial effect was exhibited by F5 formulation (non-irradiated film) against Escherichia coli and Klebsiella pneumonia; however, antibacterial activity of the films was significantly (p < 0.05) reduced after their irradiation. F5 formulation showed the highest cumulative amount of permeated drug after 24 h, while F1 (100% chitosan) showed the lowest amount of permeated drug. Non-Fickian diffusion (anomalous) was the main mode of drug release from all films. The cross-linking of films by γ-radiations improved their mechanical properties. The percentage swelling ratio was the highest in non-irradiated films having a polymeric blend (50:50). Water uptake of irradiated films was appreciably reduced as compared to non-irradiated films. Conclusion: The synthesized bionanocomposites are promising therapeutic moieties which not only improve drug permeability across but also ameliorates antibacterial potential of cefixime.","PeriodicalId":15673,"journal":{"name":"Journal of Experimental Nanoscience","volume":"17 1","pages":"389 - 419"},"PeriodicalIF":2.8,"publicationDate":"2022-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41708233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-05-31DOI: 10.1080/17458080.2022.2075545
Hui Zhao, Liyi He
Abstract Ischemic stroke results in severe disabilities due to extensive cellular loss and the resulting impairment of brain functions. Current methods for regenerating brain tissue are ineffective. Stroke treatment requires innovative therapeutic techniques that are both safe and effective. For neuronal repair, a promising alternative is using a hydrogel-based tissue engineering technique that delivers neurotrophic cytokines and cells to injured sites. However, the limited encapsulation effectiveness, less in vivo cell survival ratio and cytokine loss make this strategy difficult to implement. We aim to design a biomaterial that can efficiently construct a matrix enriching the survival of cells and minimizing loss in vivo cytokines to overcome these constraints. We report the development of genipin conjugated sericin hydrogels (Gen-SH) with a high porous morphology and a moderate swelling rate utilizing sericin, a natural silk protein. In vitro, Gen-SH aids in the attachment and development of neurons. Our results indicate that sericin is inherently neuroprotective and neurotrophic, branching and publicizing axon extension and avoiding hypoxia-induced cell death in primary neurons. Notably, the breakdown products of Gen-SH inherit these capabilities, saving the expense of cytokines. Furthermore, we show that the Lkb1–Nuak1 pathway is required for this neurotrophic impact, whereas the Bcl-2/Bax protein ratio is necessary for the neuroprotective effect. Transplanted in vivo, Gen-SH has a high percentage of cell survival and promotes cell proliferation. Taking all this information into account, it's clear that Gen-SH can serve as a viable carrier for treatment and care for ischemic stroke healing, both in terms of delivering neuronal cells and protecting them from oxidative damage.
{"title":"Fabrication of neuroprotective silk-sericin hydrogel: potential neuronal carrier for the treatment and care of ischemic stroke","authors":"Hui Zhao, Liyi He","doi":"10.1080/17458080.2022.2075545","DOIUrl":"https://doi.org/10.1080/17458080.2022.2075545","url":null,"abstract":"Abstract Ischemic stroke results in severe disabilities due to extensive cellular loss and the resulting impairment of brain functions. Current methods for regenerating brain tissue are ineffective. Stroke treatment requires innovative therapeutic techniques that are both safe and effective. For neuronal repair, a promising alternative is using a hydrogel-based tissue engineering technique that delivers neurotrophic cytokines and cells to injured sites. However, the limited encapsulation effectiveness, less in vivo cell survival ratio and cytokine loss make this strategy difficult to implement. We aim to design a biomaterial that can efficiently construct a matrix enriching the survival of cells and minimizing loss in vivo cytokines to overcome these constraints. We report the development of genipin conjugated sericin hydrogels (Gen-SH) with a high porous morphology and a moderate swelling rate utilizing sericin, a natural silk protein. In vitro, Gen-SH aids in the attachment and development of neurons. Our results indicate that sericin is inherently neuroprotective and neurotrophic, branching and publicizing axon extension and avoiding hypoxia-induced cell death in primary neurons. Notably, the breakdown products of Gen-SH inherit these capabilities, saving the expense of cytokines. Furthermore, we show that the Lkb1–Nuak1 pathway is required for this neurotrophic impact, whereas the Bcl-2/Bax protein ratio is necessary for the neuroprotective effect. Transplanted in vivo, Gen-SH has a high percentage of cell survival and promotes cell proliferation. Taking all this information into account, it's clear that Gen-SH can serve as a viable carrier for treatment and care for ischemic stroke healing, both in terms of delivering neuronal cells and protecting them from oxidative damage.","PeriodicalId":15673,"journal":{"name":"Journal of Experimental Nanoscience","volume":"17 1","pages":"362 - 376"},"PeriodicalIF":2.8,"publicationDate":"2022-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42247532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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