The cbhI gene, coding for a major cellobiohydrolase (CBHI) of Aspergillus aculeatus, was cloned and sequenced. The gene consists of 1620-bp and encodes a protein containing 540 amino acids with a calculated molecular mass of 56,723 Da. CBHI, composed of an N-terminal catalytic domain belonging to family 7 of the glycosyl hydrolases, and a C-terminal cellulose-binding domain (CBD) belonging to family I of the CBDs, showed high similarity with other fungal CBHIs, especially with that of Penicillium janthinellum. The cbhI gene transcription start points in A. aculeatus were defined by primer extension, and the putative promoter sequence was analyzed. This sequence was found to be closely related to the consensus sequences of various fungal genes. Transcription analysis by ribonuclease protection assay revealed that the cbhI gene is induced by low-molecular-weight cellooligosaccharide and repressed by glucose. The results emphasize the possibility that in the A. aculeatus cellulase system, cellobiose is the true inducer and the role of the cbhI gene lies within the cascade regulating cellulase induction.
{"title":"Cloning, nucleotide sequence, and transcriptional analysis of Aspergillus aculeatus no. F-50 cellobiohydrolase I (cbhI) gene","authors":"Goro Takada, Takashi Kawaguchi, Jun-Ichi Sumitani, Motoo Arai","doi":"10.1016/S0922-338X(97)80345-5","DOIUrl":"https://doi.org/10.1016/S0922-338X(97)80345-5","url":null,"abstract":"<div><p>The <em>cbhI</em> gene, coding for a major cellobiohydrolase (CBHI) of <em>Aspergillus aculeatus</em>, was cloned and sequenced. The gene consists of 1620-bp and encodes a protein containing 540 amino acids with a calculated molecular mass of 56,723 Da. CBHI, composed of an N-terminal catalytic domain belonging to family 7 of the glycosyl hydrolases, and a C-terminal cellulose-binding domain (CBD) belonging to family I of the CBDs, showed high similarity with other fungal CBHIs, especially with that of <em>Penicillium janthinellum</em>. The <em>cbhI</em> gene transcription start points in <em>A. aculeatus</em> were defined by primer extension, and the putative promoter sequence was analyzed. This sequence was found to be closely related to the consensus sequences of various fungal genes. Transcription analysis by ribonuclease protection assay revealed that the <em>cbhI</em> gene is induced by low-molecular-weight cellooligosaccharide and repressed by glucose. The results emphasize the possibility that in the <em>A. aculeatus</em> cellulase system, cellobiose is the true inducer and the role of the <em>cbhI</em> gene lies within the cascade regulating cellulase induction.</p></div>","PeriodicalId":15696,"journal":{"name":"Journal of Fermentation and Bioengineering","volume":"85 1","pages":"Pages 1-9"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0922-338X(97)80345-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91704883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1998-01-01DOI: 10.1016/S0922-338X(98)80095-0
Kyung-Hee Jung, Sang-Soo Kwak, Jang R. Liu
The biomass concentration in a bubble column reactor was estimated by analyzing the conductivity change during high-density culture of Catharanthus roseus hairy roots. The accumulated biomass in the reactor was estimated from the amount of nutrients consumed, which were present in the liquid medium. Liquid medium, consisting of water and nutrients, was incorporated into the biomass, in proportion to the growth of hairy roots, consequently the ratio of the volume of liquid medium to the volume of the biomass decreased continuously. To evaluate nutrient consumption, the change in biomass volume in the reactor was introduced as a new parameter for biomass estimation. After 45 d of culture, the final concentration of hairy roots was 46.5 g dry wt./l, of which the biomass volume is about 40% of the total culture volume of the reactor. A conventional conductivity method, in which the volume change of the liquid medium was not considered, resulted in a deviation (55.76 g dry wt./l) of about 20% from the actual biomass. This procedure yielded a value of 47.1 g dry wt./l of hairy roots, which is a nearly accurate estimation of the biomass concentration. This result suggests that the volume reduction of liquid medium should be considered for biomass estimation and process control for high-density plant cell cultures.
{"title":"Procedure for biomass estimation considering the change in biomass volume during high density culture of hairy roots","authors":"Kyung-Hee Jung, Sang-Soo Kwak, Jang R. Liu","doi":"10.1016/S0922-338X(98)80095-0","DOIUrl":"10.1016/S0922-338X(98)80095-0","url":null,"abstract":"<div><p>The biomass concentration in a bubble column reactor was estimated by analyzing the conductivity change during high-density culture of <em>Catharanthus roseus</em> hairy roots. The accumulated biomass in the reactor was estimated from the amount of nutrients consumed, which were present in the liquid medium. Liquid medium, consisting of water and nutrients, was incorporated into the biomass, in proportion to the growth of hairy roots, consequently the ratio of the volume of liquid medium to the volume of the biomass decreased continuously. To evaluate nutrient consumption, the change in biomass volume in the reactor was introduced as a new parameter for biomass estimation. After 45 d of culture, the final concentration of hairy roots was 46.5 g dry wt./<em>l</em>, of which the biomass volume is about 40% of the total culture volume of the reactor. A conventional conductivity method, in which the volume change of the liquid medium was not considered, resulted in a deviation (55.76 g dry wt./<em>l</em>) of about 20% from the actual biomass. This procedure yielded a value of 47.1 g dry wt./<em>l</em> of hairy roots, which is a nearly accurate estimation of the biomass concentration. This result suggests that the volume reduction of liquid medium should be considered for biomass estimation and process control for high-density plant cell cultures.</p></div>","PeriodicalId":15696,"journal":{"name":"Journal of Fermentation and Bioengineering","volume":"85 4","pages":"Pages 454-457"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0922-338X(98)80095-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91340823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1998-01-01DOI: 10.1016/S0922-338X(97)80350-9
Y. Shinohara, H. Uchiyama, O. Yagi, I. Kusakabe
{"title":"Purification and characterization of component B of a soluble methane monooxygenase from Methylocystis sp. M","authors":"Y. Shinohara, H. Uchiyama, O. Yagi, I. Kusakabe","doi":"10.1016/S0922-338X(97)80350-9","DOIUrl":"https://doi.org/10.1016/S0922-338X(97)80350-9","url":null,"abstract":"","PeriodicalId":15696,"journal":{"name":"Journal of Fermentation and Bioengineering","volume":"18 1","pages":"37-42"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87201796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1998-01-01DOI: 10.1016/S0922-338X(98)80150-5
John E. Hallsworth, Yoshiyuki Nomura, Masayoshi Iwahara
Fungal growth inhibition by ethanol was compared with that caused by five other agents of water stress (at 25, 40 and 42.5°C), using Aspergillus oryzae. Ethanol, KCl, glycerol, glucose, sorbitol, and polyethylene glycol 400 were incorporated into media at concentrations corresponding to water activity (aw) values in the range 1 to 0.75. Generally, as temperature increased there was a decrease in the aw value at which optimum growth occurred. The aw limit for growth on KCl, glycerol, glucose, sorbitol, or polyethylene glycol 400 media was about 0.85, regardless of temperature. However, the aw limit for growth on ethanol media varied between 0.97 and 0.99 aw and was temperature-dependent. Water stress accounted for up to 31, 18 and 6% of growth inhibition by ethanol at 25, 40, and 42.5°C, respectively. For media containing ethanol, the decrease in growth rate per unit of aw reduction was greater as temperature increased. However, ethanol-induced water stress remained constant regardless of temperature, suggesting that other inhibitory effects of ethanol are closely temperature-dependent. Water stress may account for considerably more than 30% of growth inhibition by ethanol in cells that remain metabolically active at higher ethanol concentrations.
{"title":"Ethanol-induced water stress and fungal growth","authors":"John E. Hallsworth, Yoshiyuki Nomura, Masayoshi Iwahara","doi":"10.1016/S0922-338X(98)80150-5","DOIUrl":"10.1016/S0922-338X(98)80150-5","url":null,"abstract":"<div><p>Fungal growth inhibition by ethanol was compared with that caused by five other agents of water stress (at 25, 40 and 42.5°C), using <em>Aspergillus oryzae</em>. Ethanol, KCl, glycerol, glucose, sorbitol, and polyethylene glycol 400 were incorporated into media at concentrations corresponding to water activity (a<sub>w</sub>) values in the range 1 to 0.75. Generally, as temperature increased there was a decrease in the a<sub>w</sub> value at which optimum growth occurred. The a<sub>w</sub> limit for growth on KCl, glycerol, glucose, sorbitol, or polyethylene glycol 400 media was about 0.85, regardless of temperature. However, the a<sub>w</sub> limit for growth on ethanol media varied between 0.97 and 0.99 a<sub>w</sub> and was temperature-dependent. Water stress accounted for up to 31, 18 and 6% of growth inhibition by ethanol at 25, 40, and 42.5°C, respectively. For media containing ethanol, the decrease in growth rate per unit of a<sub>w</sub> reduction was greater as temperature increased. However, ethanol-induced water stress remained constant regardless of temperature, suggesting that other inhibitory effects of ethanol are closely temperature-dependent. Water stress may account for considerably more than 30% of growth inhibition by ethanol in cells that remain metabolically active at higher ethanol concentrations.</p></div>","PeriodicalId":15696,"journal":{"name":"Journal of Fermentation and Bioengineering","volume":"86 5","pages":"Pages 451-456"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0922-338X(98)80150-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90539413","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Peptide ligands which bound to a model monomeric protein, bovine pancreatic ribonuclease A, could be isolated from a constrained random hexapeptide phage library. Selection was successful in a low ionic strength buffer (10 mM sodium phosphate, pH 6.0), whereas it failed in TBS (50 mM Tris-Cl, 150 mM NaCl, pH 7.5). Two of the displayed amino acid sequences from among the clones isolated were AEGACEQLDYNC and AEGACLWHDQLC. Electrostatic interaction appeared to play an important role in the binding because these phages could not bind to RNase A at a high ionic strength. The results suggest that selection in low ionic strength buffers could make possible the isolation of peptide ligands against proteins of interest which do not originally interact with another peptide or protein.
从约束的随机六肽噬菌体文库中分离出与模型单分子蛋白牛胰腺核糖核酸酶a结合的肽配体。选择在低离子强度缓冲液(10 mM磷酸钠,pH 6.0)中成功,而在TBS (50 mM Tris-Cl, 150 mM NaCl, pH 7.5)中失败。其中显示的氨基酸序列为AEGACEQLDYNC和AEGACLWHDQLC。静电相互作用似乎在结合中起重要作用,因为这些噬菌体不能在高离子强度下与RNase A结合。结果表明,在低离子强度缓冲液中选择可能使肽配体分离针对感兴趣的蛋白质,这些蛋白质最初不与另一肽或蛋白质相互作用。
{"title":"The importance of ionic strength as a parameter in screening peptide ligands from a phage display library","authors":"Yoshio Katakura, Eun Tae Lim, Setsuo Tsujii, Takeshi Omasa, Ken-Ichi Suga","doi":"10.1016/S0922-338X(98)80093-7","DOIUrl":"10.1016/S0922-338X(98)80093-7","url":null,"abstract":"<div><p>Peptide ligands which bound to a model monomeric protein, bovine pancreatic ribonuclease A, could be isolated from a constrained random hexapeptide phage library. Selection was successful in a low ionic strength buffer (10 mM sodium phosphate, pH 6.0), whereas it failed in TBS (50 mM Tris-Cl, 150 mM NaCl, pH 7.5). Two of the displayed amino acid sequences from among the clones isolated were AEGACEQLDYNC and AEGACLWHDQLC. Electrostatic interaction appeared to play an important role in the binding because these phages could not bind to RNase A at a high ionic strength. The results suggest that selection in low ionic strength buffers could make possible the isolation of peptide ligands against proteins of interest which do not originally interact with another peptide or protein.</p></div>","PeriodicalId":15696,"journal":{"name":"Journal of Fermentation and Bioengineering","volume":"85 4","pages":"Pages 447-450"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0922-338X(98)80093-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91106647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1998-01-01DOI: 10.1016/S0922-338X(98)80110-4
{"title":"Purification and characterization of β-N-acetylhexosaminidase from Streptomyces sp. no. 499","authors":"","doi":"10.1016/S0922-338X(98)80110-4","DOIUrl":"https://doi.org/10.1016/S0922-338X(98)80110-4","url":null,"abstract":"","PeriodicalId":15696,"journal":{"name":"Journal of Fermentation and Bioengineering","volume":"85 5","pages":"Page 554"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0922-338X(98)80110-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136463864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1998-01-01DOI: 10.1016/S0922-338X(99)89021-7
{"title":"Key work index","authors":"","doi":"10.1016/S0922-338X(99)89021-7","DOIUrl":"https://doi.org/10.1016/S0922-338X(99)89021-7","url":null,"abstract":"","PeriodicalId":15696,"journal":{"name":"Journal of Fermentation and Bioengineering","volume":"86 4","pages":"Page II"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0922-338X(99)89021-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136985214","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1998-01-01DOI: 10.1016/S0922-338X(99)80023-3
{"title":"Improved ε-poly-l-lysine production of an S-(2-aminoethyl)-l-cysteine resistant mutant of Streptomyces albulus","authors":"","doi":"10.1016/S0922-338X(99)80023-3","DOIUrl":"https://doi.org/10.1016/S0922-338X(99)80023-3","url":null,"abstract":"","PeriodicalId":15696,"journal":{"name":"Journal of Fermentation and Bioengineering","volume":"86 6","pages":"Page 624"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0922-338X(99)80023-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136499624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The moss Pohlia flexuosa was obtained as the protonema from its matured capsules. It was grown by shaking in modified Murashige-Skoog's medium, and the dry weight obtained was 40 mg per 20 ml medium after 15 d. The mature protonema removed Hg2+ from aqueous solutions. The higher the initial Hg2+ concentration, the higher the amount of Hg2+ removed, and the concentration factor, which is an indicator of the ability to remove heavy metals, was 2000 ml/g of dry moss at 50 μg/ml of HgCl2. The optimal pH and temperature for Hg2+ removal were 7.0 and 25°C, respectively. Sodium azide and dinitrophenol at 0.1 mM inhibited Hg2+ removal up to a relative removal of 81 and 77%, respectively. The importance of two processes, bioaccumulation and adsorption, in the removal of Hg2+ by this moss is discussed.
{"title":"Removal of mercury ion by the moss Pohlia flexuosa","authors":"Mitsuyo Kondoh , Masanori Fukuda , Masayuki Azuma , Hiroshi Ooshima , Jyoji Kato","doi":"10.1016/S0922-338X(98)80061-5","DOIUrl":"10.1016/S0922-338X(98)80061-5","url":null,"abstract":"<div><p>The moss <em>Pohlia flexuosa</em> was obtained as the protonema from its matured capsules. It was grown by shaking in modified Murashige-Skoog's medium, and the dry weight obtained was 40 mg per 20 ml medium after 15 d. The mature protonema removed Hg<sup>2+</sup> from aqueous solutions. The higher the initial Hg<sup>2+</sup> concentration, the higher the amount of Hg<sup>2+</sup> removed, and the concentration factor, which is an indicator of the ability to remove heavy metals, was 2000 ml/g of dry moss at 50 μg/ml of HgCl<sub>2</sub>. The optimal pH and temperature for Hg<sup>2+</sup> removal were 7.0 and 25°C, respectively. Sodium azide and dinitrophenol at 0.1 mM inhibited Hg<sup>2+</sup> removal up to a relative removal of 81 and 77%, respectively. The importance of two processes, bioaccumulation and adsorption, in the removal of Hg<sup>2+</sup> by this moss is discussed.</p></div>","PeriodicalId":15696,"journal":{"name":"Journal of Fermentation and Bioengineering","volume":"86 2","pages":"Pages 197-201"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0922-338X(98)80061-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84883748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Nitrous oxide (N2O) is emitted from wastewater treatment processes. It is known as a greenhouse gas that contributes to global warming (over 200 times more per molecule than carbon dioxide) and to the destruction of the ozone layer. It is therefore of great importance to develop technology that can suppress N2O emission. The effects of an anoxic period on N2O emission and nitrogen removal were investigated in an actual domestic wastewater treatment plant. When operated with intermittent aeration, most of the N2O was emitted into the atmosphere during the aerobic period. N2O emission from the intermittent process was estimated to be 0.43–1.89 g N2O person−1 year−1. Maintaining a dissolved oxygen (DO) concentration of over 0.5 mg l−1 during the aerobic period resulted in the complete conversion of the influent NH4-N to NO3-N and a 60-min anoxic period was sufficient for denitrification to be completed. The findings show that an optimum combination of aerobic and anoxic conditions and their suitable control are very important for improving nitrogen removal efficiency and controlling N2O emission.
一氧化二氮(N2O)是从废水处理过程中排放出来的。众所周知,它是一种温室气体,会导致全球变暖(每分子比二氧化碳多200多倍),并破坏臭氧层。因此,开发抑制N2O排放的技术具有重要意义。在实际生活污水处理厂中,研究了缺氧周期对N2O排放和氮去除的影响。间歇曝气时,大部分N2O在好氧期排放到大气中。间歇过程的N2O排放量估计为0.43-1.89 g N2O人−1年−1。在好氧期间,将溶解氧(DO)浓度维持在0.5 mg l - 1以上,可使进水NH4-N完全转化为NO3-N, 60分钟的缺氧期足以完成反硝化。结果表明,好氧和缺氧条件的最佳组合及其控制对提高脱氮效率和控制N2O排放具有重要意义。
{"title":"Nitrogen removal and N2O emission in a full-scale domestic wastewater treatment plant with intermittent aeration","authors":"Yuzuru Kimochi , Yuhei Inamori , Motoyuki Mizuochi , Kai-Qin Xu , Masatoshi Matsumura","doi":"10.1016/S0922-338X(98)80114-1","DOIUrl":"10.1016/S0922-338X(98)80114-1","url":null,"abstract":"<div><p>Nitrous oxide (N<sub>2</sub>O) is emitted from wastewater treatment processes. It is known as a greenhouse gas that contributes to global warming (over 200 times more per molecule than carbon dioxide) and to the destruction of the ozone layer. It is therefore of great importance to develop technology that can suppress N<sub>2</sub>O emission. The effects of an anoxic period on N<sub>2</sub>O emission and nitrogen removal were investigated in an actual domestic wastewater treatment plant. When operated with intermittent aeration, most of the N<sub>2</sub>O was emitted into the atmosphere during the aerobic period. N<sub>2</sub>O emission from the intermittent process was estimated to be 0.43–1.89 g N<sub>2</sub>O person<sup>−1</sup> year<sup>−1</sup>. Maintaining a dissolved oxygen (DO) concentration of over 0.5 mg <em>l</em><sup>−1</sup> during the aerobic period resulted in the complete conversion of the influent NH<sub>4</sub>-N to NO<sub>3</sub>-N and a 60-min anoxic period was sufficient for denitrification to be completed. The findings show that an optimum combination of aerobic and anoxic conditions and their suitable control are very important for improving nitrogen removal efficiency and controlling N<sub>2</sub>O emission.</p></div>","PeriodicalId":15696,"journal":{"name":"Journal of Fermentation and Bioengineering","volume":"86 2","pages":"Pages 202-206"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0922-338X(98)80114-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85162979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}