Journal of endodontics最新文献

{"title":"Endodontic Inflammatory Disease and Future Cardiovascular Events and Mortality: A Report from the PAROKRANK Study","authors":"","doi":"10.1016/j.joen.2024.05.003","DOIUrl":"10.1016/j.joen.2024.05.003","url":null,"abstract":"<div><h3>Introduction</h3><p>Prospective studies assessing the relation between endodontic inflammatory disease and subsequent cardiovascular events are few. The present aim was to explore associations between endodontic variables and future cardiovascular events in patients with myocardial infarction and matched controls participating in the PAROKRANK (Periodontitis and Its Relation to Coronary Artery Disease) study.</p></div><div><h3>Methods</h3><p>Eight-hundred five patients hospitalized for a first myocardial infarction and 805 controls were recruited between 2010 and 2014. Signs of endodontic inflammatory disease were assessed in panoramic radiographs taken at baseline. Mortality and morbidity data during the approximately 8 years of follow up were obtained from national registries. The risk for future cardiovascular events (first of mortality and nonfatal myocardial infarction, stroke, or hospitalization for heart failure) was analyzed with the log-rank test and Cox proportional hazards regression adjusted for the following confounders: sex, age, smoking, myocardial infarction, diabetes, education, marital status, family history of cardiovascular disease, and marginal periodontitis.</p></div><div><h3>Results</h3><p>In total, 285 future events were observed during the follow-up period. Unadjusted analyses revealed that ≥1 root-filled tooth increased the risk of a future event. After adjustment, the number of remaining teeth and non–root-filled teeth decreased the risk of future events, whereas a higher Decayed, Missing and Filled Teeth score increased the risk and ≥1 primary apical periodontitis decreased the risk of suffering cardiovascular events. A higher Decayed, Missing and Filled Teeth score and decayed teeth increased the risk of all-cause mortality.</p></div><div><h3>Conclusions</h3><p>Tooth loss is a strong indicator of an increased risk for future cardiovascular events. Root-filled teeth seem of limited value as a risk indicator when accounting for other risk factors. The potential effect of dental interventions on future events should be assessed in future research.</p></div>","PeriodicalId":15703,"journal":{"name":"Journal of endodontics","volume":"50 8","pages":"Pages 1073-1081.e3"},"PeriodicalIF":3.5,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0099239924002838/pdfft?md5=dc714004fa72ed281857de223b9c9938&pid=1-s2.0-S0099239924002838-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141065727","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Literature Review of the Effect of Heat on the Physical-Chemical Properties of Calcium Silicate–Based Sealers","authors":"","doi":"10.1016/j.joen.2024.04.017","DOIUrl":"10.1016/j.joen.2024.04.017","url":null,"abstract":"<div><h3>Introduction</h3><p>Recently, calcium silicate–based sealers (CSSs) have gained popularity in endodontic practice due to their biocompatibility and antimicrobial properties. They are considered viable alternatives to epoxy resin-based sealers. With the increased use of CSSs and warm vertical compaction techniques in root canal treatment, evaluating the impact of heat on CSSs properties is essential, therefore this review aimed to present a qualitative synthesis of available <em>in vitro</em> studies assessing the impact of heat on the physical-chemical properties of CSSs.</p></div><div><h3>Methods</h3><p>Following the Preferred Reporting Items for Systematic Reviews and Meta-Analysis 2020 guidelines, a systematic advanced electronic search was performed in Scopus, Embase, Medline (via PubMed), Web of Science, and Cochrane databases in November 2023 and updated in April 2024. <em>In vitro</em> studies that evaluated the physical-chemical properties of CSSs were eligible. PRILE 2021 guidelines were used for the assessment of the risk of bias–included studies.</p></div><div><h3>Results</h3><p>The search identified a total of 6421 preliminary results and 10 studies were included for qualitative assessment. Eleven different physiochemical properties were assessed by the included studies. Setting time and flow were the most evaluated property among the studies. A qualitative synthesis of the evidence on each property is presented.</p></div><div><h3>Conclusions</h3><p>Based on the <em>in vitro</em> studies assessed in the present systematic review, results reveal that exposing CSSs to heat can accelerate their setting time, reduce their flow, and increase their film thickness. Concerns persist regarding solubility, viscosity, radiopacity, dimensional change, microhardness, porosity, and compressive strength, requiring further research. Certain CSSs, such as MTA Fillapex and Endosequence BC sealer HiFlow, show minimal changes under heat, making them potential candidates for warm filling techniques.</p></div>","PeriodicalId":15703,"journal":{"name":"Journal of endodontics","volume":"50 8","pages":"Pages 1044-1058.e5"},"PeriodicalIF":3.5,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0099239924002814/pdfft?md5=dd045142c50c7c8b3f7bb7b142b4e345&pid=1-s2.0-S0099239924002814-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140904333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Insights into the August 2024 Issue of the Journal of Endodontics","authors":"","doi":"10.1016/j.joen.2024.06.014","DOIUrl":"10.1016/j.joen.2024.06.014","url":null,"abstract":"","PeriodicalId":15703,"journal":{"name":"Journal of endodontics","volume":"50 8","pages":"Pages 1033-1035"},"PeriodicalIF":3.5,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141476678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Associate Registry/Donor Roll","authors":"","doi":"10.1016/S0099-2399(24)00390-X","DOIUrl":"10.1016/S0099-2399(24)00390-X","url":null,"abstract":"","PeriodicalId":15703,"journal":{"name":"Journal of endodontics","volume":"50 8","pages":"Pages 1163-1194"},"PeriodicalIF":3.5,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141979186","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Access to Care Considerations for the Endodontic Treatment of Immature Permanent Teeth: A National Survey of Pediatric Dentists and Endodontists","authors":"","doi":"10.1016/j.joen.2024.05.009","DOIUrl":"10.1016/j.joen.2024.05.009","url":null,"abstract":"<div><h3>Introduction</h3><p>This study aimed to investigate access to care and financial considerations associated with the endodontic treatment of immature permanent teeth.</p></div><div><h3>Methods</h3><p>Surveys were distributed to endodontists (<em>n</em> = 2,457) and pediatric dentists (<em>n</em> = 3,974) in the United States. Data were analyzed using X² analysis and logistic regression. The level of significance was set to 0.05.</p></div><div><h3>Results</h3><p>The response rate was 13% (<em>n</em> = 840). Respondent specialist groups were similar by age and years since specialty residency completion, but significantly different with regard to primary practice setting (eg private practice, Federally Qualified Health Center, hospital), (<em>P</em> = .001). The majority (91%) of respondents reported participation with dental insurance. Pediatric dentists (69%) were significantly more likely than endodontists (17%) to participate with public-payer dental insurance (<em>P</em> &lt; .001). The majority of respondents (82%) indicated that patients reported economic factors (time or money) as a barrier to accessing endodontic treatment. Pediatric dentists were significantly more likely to consider economic factors when planning for treatment (<em>P</em> &lt; .001). Pediatric dentists were more likely than endodontists to have the opinion that endodontic procedures for treatment of necrotic immature permanent teeth should cost less than root canal therapy (apexification, <em>P</em> &lt; .001; regenerative endodontic procedures, <em>P</em> = .002). Pediatric dentists (33%) reported encountering barriers when attempting to refer their patients to an endodontist. Inability to find an endodontist that participates with dental insurance was the most frequently cited barrier.</p></div><div><h3>Conclusions</h3><p>Limited clinician participation with dental insurance and gaps in insurance coverage for endodontic procedures appear to contribute to access to care barriers for pediatric patients.</p></div>","PeriodicalId":15703,"journal":{"name":"Journal of endodontics","volume":"50 8","pages":"Pages 1100-1107"},"PeriodicalIF":3.5,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0099239924003042/pdfft?md5=3151403fe29d7edaa4af7e540070a0c9&pid=1-s2.0-S0099239924003042-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141135842","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Carbon Nanotubes Induce Mineralization of Human Cementoblasts","authors":"","doi":"10.1016/j.joen.2024.04.014","DOIUrl":"10.1016/j.joen.2024.04.014","url":null,"abstract":"<div><h3>Introduction</h3><p>Carbon nanotubes<span> (CNT) are 1 of the allotropes of carbon with unique properties. CNT shows good bone-tissue compatibility and has been reported to induce osteogenesis; therefore, it is regarded as an ideal material in a wide range of applications. However, the therapeutic effect of CNT-containing materials in the healing of apical periodontal tissue is unknown. The purpose of this study was to clarify the effect of CNT on the proliferation and mineralization<span> of the human cementoblast cell line (HCEM).</span></span></p></div><div><h3>Methods</h3><p>The proliferation of HCEM cells with CNT stimulation was assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay performed from 24–72 hours. Calcium deposition levels were evaluated by alizarin red S staining on days 7 and 10, and mineralization-related gene expression was examined by quantitative real-time polymerase chain reaction on days 3, 7, and 10. Scanning electron microscopy was used to observe the culture with CNT on day 14.</p></div><div><h3>Results</h3><p><span>CNT showed no cytotoxicity to HCEM cell proliferation<span>. Treatment was performed with mineralization medium, CNT-induced HCEM mineralization on day 7, and increased calcium deposition on days 7 and 14. Messenger RNA expression of </span></span>alkaline phosphatase<span> was significantly increased throughout the experimental period, and bone sialoprotein<span> was significantly increased on day 3 by CNT, whereas no effect was found on mRNA expression of type I collagen. CNT was observed in attachment to the cell surface on day 14.</span></span></p></div><div><h3>Conclusions</h3><p>CNT promotes the mineralization of HCEM cells, indicating the potential as a new bioactive component for apical periodontal tissue regeneration materials through the regulation of cementoblast mineralization.</p></div>","PeriodicalId":15703,"journal":{"name":"Journal of endodontics","volume":"50 8","pages":"Pages 1117-1123"},"PeriodicalIF":3.5,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140891905","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Heparan Sulfate on Vasculogenesis and Dentinogenesis of Dental Pulp Stem Cells","authors":"","doi":"10.1016/j.joen.2024.04.015","DOIUrl":"10.1016/j.joen.2024.04.015","url":null,"abstract":"<div><h3>Introduction</h3><p>Heparan sulfate (HS) is a major component of dental pulp tissue. We previously reported that inhibiting HS biosynthesis impedes endothelial differentiation of dental pulp stem cells (DPSCs). However, the underlying mechanisms by which exogenous HS induces DPSC differentiation and pulp tissue regeneration remain unknown. This study explores the impact of exogenous HS on vasculogenesis and dentinogenesis of DPSCs both <em>in vitro</em> and <em>in vivo</em>.</p></div><div><h3>Methods</h3><p>Human-derived DPSCs were cultured in endothelial and odontogenic differentiation media and treated with HS. Endothelial differentiation of DPSCs was investigated by real-time polymerase chain reaction and capillary sprouting assay. Odontogenic differentiation was assessed through real-time polymerase chain reaction and detection of mineralized dentin-like deposition. Additionally, the influence of HS on pulp tissue was assessed with a direct pulp capping model, in which HS was delivered to exposed pulp tissue in rats. Gelatin sponges were loaded with either phosphate-buffered saline or 10¹–10² μg/mL HS and placed onto the pulp tissue. Following a 28-day period, tissues were investigated by histological analysis and micro–computed tomography imaging.</p></div><div><h3>Results</h3><p>HS treatment markedly increased expression levels of key endothelial and odontogenic genes, enhanced the formation of capillary-like structures, and promoted the deposition of mineralized matrices. Treatment of exposed pulp tissue with HS in the <em>in vivo</em> pulp capping study induced formation of capillaries and reparative dentin.</p></div><div><h3>Conclusions</h3><p>Exogenous HS effectively promoted vasculogenesis and dentinogenesis of DPSCs <em>in vitro</em> and induced reparative dentin formation <em>in vivo</em>, highlighting its therapeutic potential for pulp capping treatment.</p></div>","PeriodicalId":15703,"journal":{"name":"Journal of endodontics","volume":"50 8","pages":"Pages 1108-1116"},"PeriodicalIF":3.5,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0099239924002784/pdfft?md5=4c09851d169e5090a824ba30e03743b2&pid=1-s2.0-S0099239924002784-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140891866","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metatranscriptome and Resistome of the Endodontic Microbiome","authors":"","doi":"10.1016/j.joen.2024.03.015","DOIUrl":"10.1016/j.joen.2024.03.015","url":null,"abstract":"<div><h3>Introduction</h3><p><span>In this study, we used metatranscriptomics for the first time to investigate microbial composition, functional signatures, and </span>antimicrobial resistance<span> gene expression in endodontic infections.</span></p></div><div><h3>Methods</h3><p><span><span><span>Root canal samples were collected from ten teeth, including five primary and five persistent/secondary endodontic infections. </span>RNA from endodontic samples was extracted, and </span>RNA sequencing was performed on a NovaSeq6000 system (Illumina). Taxonomic analysis was performed using the Kraken2 bacterial database. Then, sequences with a taxonomic classification were annotated against the Universal Protein Knowledgebase for functional annotation and the Comprehensive </span>Antibiotic Resistance Database for AR-like gene identification.</p></div><div><h3>Results</h3><p><span><span>Proteobacteria<span><span>, Bacteroidetes, </span>Firmicutes, and </span></span>Actinobacteria<span><span> represented the dominant phyla, whereas Fusobacteria, </span>Spirochetes<span>, and Synergistetes were among the nondominant phyla. The top ten species were mainly represented by obligate (or quasiobligate) anaerobes, including Gram-negative (eg, </span></span></span><span><span>Capnocytophaga</span></span> sp. oral taxon 323, <span><span>Fusobacterium nucleatum</span></span>, <span><span>Prevotella</span><em> intermedia</em></span>, <span><span>Prevotella</span><em> oris</em></span>, <span><span>Tannerella forsythia</span><em>,</em></span> and <span><span>Tannerella</span></span> sp. oral taxon HOT−286) and Gram-positive species (eg, <span><span>Olsenella</span><em> uli</em></span> and <span><span>Parvimonas micra</span></span><span><span><span>). Transcripts encoding moonlighting proteins (eg, glycolytic proteins, translational </span>elongation factors, </span>chaperonin<span><span>, and heat shock proteins) were highly expressed, potentially affecting bacterial adhesion<span>, biofilm formation, host defense evasion, and inflammation induction. Endodontic bacteria expressed genes conferring resistance to antibiotic<span> classes commonly used in dentistry<span>, with a high prevalence and expression of tetracycline and </span></span></span></span>lincosamide resistance genes. Antibiotic efflux and antibiotic target alteration/protection were the main resistance mechanisms.</span></span></p></div><div><h3>Conclusions</h3><p>Metatranscriptomics revealed the activity of potential endodontic pathogens<span>, which expressed putative virulence factors and a wide diversity of genes potentially involved in AR.</span></p></div>","PeriodicalId":15703,"journal":{"name":"Journal of endodontics","volume":"50 8","pages":"Pages 1059-1072.e4"},"PeriodicalIF":3.5,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140891869","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Letter to the Editor Regarding, “Two-Dimensional Radiographs and CBCT Assessment of Concentrated Growth Factor and Platelet-Rich Fibrin Scaffolds in Regenerative Endodontic Treatment of Immature Incisors with Periapical Radiolucency: A Randomized Clinical Trial”","authors":"","doi":"10.1016/j.joen.2024.03.011","DOIUrl":"10.1016/j.joen.2024.03.011","url":null,"abstract":"","PeriodicalId":15703,"journal":{"name":"Journal of endodontics","volume":"50 8","pages":"Page 1036"},"PeriodicalIF":3.5,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141306090","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electrochemical Properties of Nickel-Titanium Rotary Endodontic Instruments","authors":"","doi":"10.1016/j.joen.2024.05.004","DOIUrl":"10.1016/j.joen.2024.05.004","url":null,"abstract":"<div><h3>Introduction</h3><p>Nickel-titanium rotary endodontic<span> files have been commercially available for decades, but more recent innovations have introduced heat-treated and surface-treated files. This study investigated the corrosion properties of various nickel-titanium files in normal saline and sodium hypochlorite (NaOCl).</span></p></div><div><h3>Methods</h3><p>Ten different file brands of size 40 with a 0.04 taper were subjected to electrochemical testing in 0.9% NaCl (saline) and 5.25% NaOCl at room temperature. The Open Circuit Potential (OCP) was observed for 1 hour followed by a cyclic polarization test from −300 to 700 mV and back to −300 mV (vs OCP). Nonparametric ANOVA and a pairwise comparison (<em>P</em> &lt; .05) were used for statistical analysis of the OCP at 1 hour and the corrosion current (Icorr) obtained via the cyclic polarization test.</p></div><div><h3>Results</h3><p>Significant differences (<em>P</em> &lt; .05) were found between files with respect to OCP and Icorr in both solutions. Nine files exhibited significantly greater (<em>P</em><span> &lt; .05) Icorrs in NaOCl than in saline. Conversely, pitting corrosion was observed in the saline solution but not NaOCl. Weak and/or moderate correlations existed between OCP and Icorr measures in the 2 solutions.</span></p></div><div><h3>Conclusion</h3><p>Significant differences in electrochemical properties were observed among the 10 brands of files. Overall, there was not a clear trend between conventional, heat-treated, or surface-treated files among OCP or Icorr in either solution.</p></div>","PeriodicalId":15703,"journal":{"name":"Journal of endodontics","volume":"50 8","pages":"Pages 1143-1150"},"PeriodicalIF":3.5,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140957827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}