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Antimicrobial Silk Fibroin Methacrylated Scaffolds for Regenerative Endodontics. 用于牙髓再生的抗菌蚕丝纤维甲基丙烯酸酯支架
IF 3.5 2区 医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE Pub Date : 2024-08-21 DOI: 10.1016/j.joen.2024.08.004
Ramyasaketha Narayanam, Lais M Cardoso, Alexandre H Dos Reis-Prado, Ana Beatriz G de Carvalho, Caroline Anselmi, Abdel H Mahmoud, J Christopher Fenno, Renan Dal-Fabbro, Marco C Bottino

Introduction: Recognizing the necessity of novel disinfection strategies for improved bacterial control to ultimately favor tissue regeneration, this study developed and characterized antibiotics-laden silk fibroin methacrylated (SilkMA) scaffolds for regenerative endodontics.

Methods: SilkMA-based solutions (10% w/v) containing Clindamycin (CLI) or Tinidazole (TIN) (0 - control; 5, 10, or 15% w/w) or the combination of both drugs (BiMix CLI/TIN 10%) were electrospun and photocrosslinked. Morphology and composition were assessed using scanning electron microscopy and Fourier-transform infrared spectroscopy. Additionally, swelling and degradation profiles were also determined. Cytotoxicity was evaluated in stem cells from apical papilla. Antibacterial efficacy was tested using direct and indirect contact assays against Aggregatibacter actinomycetemcomitans/Aa, Actinomyces naeslundii/An, Enterococcus faecalis/Ef, and Fusobacterium nucleatum/Fn. E. faecalis biofilm inhibition on dentin discs was specifically evaluated for BiMix-laden scaffolds. Data were statistically analyzed with a significance level of 5%.

Results: Scanning electron microscopy revealed that all scaffolds had similar characteristics, including fiber morphology and bead absence. Fourier-transform infrared spectroscopy showed the incorporation of CLI and TIN into the fibers and in BiMix scaffolds. Antibiotic-laden scaffolds exhibited lower swelling capacity than the control and were degraded entirely after 45 days. Scaffolds laden with CLI, TIN, or BiMix throughout all time points did not reduce stem cells from apical papilla's viability. CLI-laden scaffolds inhibited the growth of Aa, An, and Ef, while TIN-laden scaffolds inhibited Fn growth. BiMix-laden scaffolds significantly inhibited Aa, An, Ef, and Fn in direct contact, and their aliquots inhibited An and Fn through indirect contact, with additional biofilm inhibition against Ef.

Conclusions: BiMix-laden SilkMA scaffolds are cytocompatible and exhibit antimicrobial effects against endodontic pathogens, indicating their therapeutic potential as a drug delivery system for regenerative endodontics.

简介:由于认识到新型消毒策略对改善细菌控制以最终促进组织再生的必要性,本研究开发了含抗生素的甲基丙烯酸丝纤维素(SilkMA)支架,并对其进行了表征:方法:将含有克林霉素(CLI)或替硝唑(TIN)(0 - 对照组;5、10 或 15% w/w)或两种药物组合(BiMix CLI/TIN 10% )的丝纤维甲基丙烯酸甲酯(SilkMA)溶液(10% w/v)进行电纺和光交联。使用扫描电子显微镜(SEM)和傅立叶变换红外光谱(FTIR)对形态和成分进行评估。此外,还测定了膨胀和降解曲线。在顶端乳头干细胞(SCAPs)中评估了细胞毒性。使用直接和间接接触试验对放线菌(Aggregatibacter actinomycetemcomitans/Aa)、奈氏放线菌(Actinomyces naeslundii/An)、粪肠球菌(Enterococcus faecalis/Ef)和核酸镰刀菌(Fusobacterium nucleatum/Fn)进行了抗菌效果测试。粪肠球菌对牙本质圆盘上生物膜的抑制作用专门针对含有 BiMix 的支架进行了评估。对数据进行了统计分析,显著性水平为 5%:扫描电子显微镜显示,所有支架都具有相似的特征,包括纤维形态和微珠缺失。傅立叶变换红外光谱(FTIR)显示,纤维和 BiMix 支架中含有 CLI 和 TIN。与对照组相比,添加抗生素的支架表现出较低的膨胀能力,并在 45 天后完全降解。在所有时间点上,含有 CLI、TIN 或 BiMix 的支架都不会降低 SCAP 的活力。含有 CLI 的支架抑制了 Aa、An 和 Ef 的生长,而含有 TIN 的支架则抑制了 Fn 的生长。含有生物混合物的支架通过直接接触显著抑制了 Aa、An、Ef 和 Fn 的生长,其等分物通过间接接触抑制了 An 和 Fn 的生长,并对 Ef 产生了额外的生物膜抑制作用:BiMix负载的SilkMA支架具有细胞相容性,对牙髓病原体有抗菌作用,表明其作为再生牙髓治疗药物输送系统的治疗潜力。
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引用次数: 0
Effect of Calcium Hydroxide as an Intracanal Medication on Dentine Fracture Resistance: A Systematic Review and Network Meta-Analysis. 氢氧化钙作为牙槽骨内药物对牙本质抗折性的影响:系统综述和网络荟萃分析。
IF 3.5 2区 医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE Pub Date : 2024-08-20 DOI: 10.1016/j.joen.2024.08.005
Chayanit Sunlakawit, Chitpol Chaimanakarn, Natchalee Srimaneekarn, Sittichoke Osiri

Introduction: Bacterial infections necessitate effective root canal disinfection during endodontic therapy. Calcium hydroxide (CH), a widely used intracanal medication, shows conflicting effects on dentine fracture resistance in different studies. This study aimed to perform a comprehensive systematic review and network meta-analysis to evaluate the effects of CH on the fracture resistance of dentine in human teeth across different periods.

Methods: PubMed, EMBASE, MEDLINE (EBSCO), Cochrane Library, Scopus, Google Scholar, and ProQuest databases were exhaustively searched to identify studies published until February 29, 2024, with no language restrictions. Laboratory studies that investigated dentine fracture resistance in human permanent teeth following intracanal CH exposure were included. The risk of bias was evaluated using modified criteria derived from previous studies. Network meta-analysis was performed using the frequentist method. Prespecified subgroup analyses focused on simulating immature teeth.

Results: The search yielded 2,265 studies from all databases, and 27 met the inclusion criteria, involving 3,879 teeth or roots. The overall results from network meta-analysis indicated that CH affected the fracture resistance of human dentine. Subgroup analysis revealed that the duration influencing fracture resistance was ≥4 weeks for mature teeth, but no specific time limitation for immature teeth. The overall quality of the evidence showed a moderate to high risk of bias.

Conclusions: Short-term use of CH does not compromise human root dentine strength, supporting its routine application in clinical practice. CH remains a viable treatment strategy for over 4 weeks of medication needs but requires carefully considering the associated risks and benefits emphasizing on preservation and disease resolution.

Registration: PROSPERO database (CRD 42024513199).

引言细菌感染要求在牙髓治疗过程中进行有效的根管消毒。氢氧化钙(CH)是一种广泛使用的根管内药物,不同的研究显示其对牙本质抗折性的影响存在冲突。本研究旨在进行全面的系统综述和网络荟萃分析,以评估不同时期氢氧化钙对人类牙齿牙本质抗折性的影响:对 PubMed、EMBASE、MEDLINE (EBSCO)、Cochrane Library、Scopus、Google Scholar 和 ProQuest 数据库进行了详尽检索,以确定 2024 年 2 月 29 日之前发表的研究,语言不限。纳入了调查人类恒牙牙本质抗折性的实验室研究。根据以往研究的修改标准对偏倚风险进行了评估。采用频数法进行网络荟萃分析。预设的亚组分析侧重于模拟未成熟的牙齿:搜索结果显示,所有数据库中共有 2265 项研究,27 项符合纳入标准,涉及 3879 颗牙齿或牙根。网络荟萃分析的总体结果表明,CH 会影响人类牙本质的抗折断性。亚组分析表明,影响成熟牙齿抗折性的持续时间≥4周,但对未成熟牙齿没有具体的时间限制。证据的总体质量显示存在中度到高度的偏倚风险:短期使用CH不会影响牙根牙本质强度,支持其在临床实践中的常规应用。对于超过 4 周的用药需求,CH 仍然是一种可行的治疗策略,但需要仔细考虑相关的风险和益处,重点是保护和解决疾病问题。
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引用次数: 0
Engineered Immunomodulatory Nanoparticles Inhibit Root Resorption and Ankylosis. 工程免疫调节纳米粒子抑制牙根吸收和强直。
IF 3.5 2区 医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE Pub Date : 2024-08-17 DOI: 10.1016/j.joen.2024.08.006
Rajeshwari Hadagalu Revana Siddappa, Emily Bishop, Aiman Ali, Marco Magalhaes, Anil Kishen

Introduction: External root resorption following avulsion injury is a complex process wherein differentiation of macrophages (Mϕ) to multinucleated osteoclasts is temporally regulated by resident periodontal fibroblasts (PDLF). The current study aims to assess the effect of engineered bioactive chitosan nanoparticles (CSNP), sustained released dexamethasone conjugated CSNP (CS-DEX) and CSNP functionalized with photosensitizer Rose Bengal (CSRB) for application in root resorption using an in-vitro PDLF-Mϕ direct coculture model and in-vivo delayed reimplantation model.

Methods: PDLF-Mϕ direct coculture system was exposed to lipopolysaccharide (LPS), macrophage colony stimulating factor, receptor activator of nuclear factor kappa β ligand with or without CSNP/CS-DEX for 7 days. Clastic differentiation was assessed by tartrate resistant acid phosphatase (TRAP) staining on day 7. On day 2 and 7, immunofluorescence analysis was conducted to assess the expression of Mϕ polarization markers (CD80, CD206), multinucleation markers (NFATc1, STAT6) in Mϕ and matricellular protein periostin in PDLF and cytokine profiling in cell culture supernatants. Delayed replantation model with extraoral air dry/LPS exposure for 1h followed by root surface treatment with CS-DEX/CSRB was used in Wistar rats. After 21 days, rats were euthanized for histologic and immunofluorescence analysis. Statistical analysis one-way ANOVA with Tukey's multiple comparisons was used to analyze the data (P < .05).

Results: CS-DEX significantly reduced TRAP+ multinucleated cells and CSNP treatment showed no TRAP+ cells. Immunofluorescence analysis showed that CSNP/CS-DEX reduced CD80, NFATc1 and STAT6 expression and increased periostin as expressed by fluorescence intensity. CSNP/CS-DEX significantly reduced TNFα, MMP9 and increased IL10, TGFβ1. Osteoprotegerin was upregulated only by CSNP. Root surface treatment in delayed replantation model showed that CS-DEX and CSRB substantially reduced the degree of resorption and ankylosis. Further, CD80, CD206, and MMP2 expression in groups with root surface treatment with CS-DEX and CSRB was lower than airdry/LPS group and similar to healthy control and NFATc1, STAT6, and MMP9 expressions were lower than healthy control.

Conclusion: The engineered nanosized immunomodulatory bioactive materials chitosan nanoparticles functionalized with photosensitizer and dexamethasone effectively reduced the clastic differentiation of Mϕ in in-vitro coculture and minimized the resorption and ankylosis in a delayed reimplantation model. These biomaterials have the potential to serve as root modification agents, promoting favorable healing outcomes in cases of dental avulsion.

导言:撕脱伤后的牙根外吸收是一个复杂的过程,其中巨噬细胞(Mφ)向多核破骨细胞的分化受驻留牙周成纤维细胞(PDLF)的时间调控。本研究旨在利用体外 PDLF-Mφ 直接共培养模型和体内延迟再植模型,评估工程生物活性壳聚糖纳米颗粒(CSNP)、地塞米松共轭缓释 CSNP(CS-DEX)和光敏剂玫瑰红功能化 CSNP(CSRB)应用于牙根吸收的效果:方法:将 PDLF-Mφ 直接共培养系统暴露于 LPS、MCSF、RANKL(含或不含 CSNP/CS-DEX)7 天。第 7 天,通过酒石酸抗性酸性磷酸酶(TRAP)染色评估碎屑分化情况。在第 2 天和第 7 天,进行免疫荧光分析,以评估 Mφ 极化标记(CD80、CD206)、Mφ 中多核标记(NFATc1、STAT6)和 PDLF 中垫细胞蛋白 periostin 的表达,以及细胞培养上清液中细胞因子的分析。在 Wistar 大鼠口外风干/LPS 暴露 1 小时,然后用 CS-DEX/CSRB 进行根表面处理的延迟再植模型。21 天后,将大鼠安乐死,进行组织学和免疫荧光分析。统计分析 采用单因素方差分析和 Tukey's 多重比较来分析数据(pResults:CS-DEX 能明显减少 TRAP+ 多核细胞,而 CSNP 处理则没有 TRAP+ 细胞。免疫荧光分析表明,CSNP/CS-DEX 减少了 CD80、NFATc1 和 STAT6 的表达,并增加了以荧光强度表示的骨膜增生蛋白。CSNP/CS-DEX 能明显降低 TNFα、MMP9 的表达,增加 IL10、TGFβ1 的表达。只有 CSNP 上调了 OPG。延迟再植模型中的根面处理表明,CS-DEX和CSRB可大大降低吸收和强直的程度。此外,使用 CS-DEX 和 CSRB 进行牙根表面处理的组中,CD80、CD206 和 MMP2 的表达低于风干/LPS 组,与健康对照组相似;NFATc1、STAT6 和 MMP9 的表达低于健康对照组:用光敏剂和地塞米松功能化的工程纳米免疫调节生物活性材料壳聚糖纳米颗粒能有效减少体外共培养中Mφ的碎屑分化,并在延迟再植模型中将吸收和强直降至最低。这些生物材料具有作为牙根改良剂的潜力,可促进牙齿脱落病例的良好愈合。
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引用次数: 0
Response to Letter From SAVADORI. 亲爱的贾迪诺博士和萨瓦多利博士。
IF 3.5 2区 医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE Pub Date : 2024-08-14 DOI: 10.1016/j.joen.2024.08.001
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引用次数: 0
Canalis Sinuosus Mimicking Periapical Pathology on, Radiographic Assessment 窦管模仿根尖周围病变的放射学评估。
IF 3.5 2区 医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE Pub Date : 2024-08-01 DOI: 10.1016/j.joen.2024.04.011

The canalis sinuosus is an anatomical variation whereby the infraorbital canal sometimes generates a small, lateral branch (canal) close to its midpoint, to allow the passage of the anterior superior alveolar neurovascular bundle in the anterior maxilla. This article focuses on an incidental finding of this variant, in a 74-year-old Trinidadian female of Afro-Caribbean descent with an endodontic presenting complaint. The canalis sinuosus shadow on conventional radiography resulted in uncertainty as to the offending tooth until a 3-dimensional scan was undertaken in this region. This report will discuss the implications of the presence of this canal from radiologic, endodontic, and surgical perspectives.

眶下窦(Canalis sinuosus,CS)是一种解剖变异,眶下管有时会在靠近中点的位置产生一个小的侧支(管),以便让上颌骨前部的前-上-牙槽(ASA)神经血管束通过。本文主要介绍在一名 74 岁的特立尼达女性牙髓病患者身上偶然发现的这种变异。在对该区域进行三维扫描之前,常规放射线检查发现的窦道阴影导致了对违规牙齿的不确定性。本报告将从放射学、牙髓学和外科角度讨论该牙髓管存在的意义。
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引用次数: 0
Development of Chlorhexidine-loaded Lipid Nanoparticles Incorporated in a Bioceramic Endodontic Sealer 在生物陶瓷牙髓封闭剂中加入氯己定脂质纳米粒子的开发。
IF 3.5 2区 医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE Pub Date : 2024-08-01 DOI: 10.1016/j.joen.2024.04.012

Introduction

This study aimed to assess BioRoot RCS (BR) incorporating liposomal chlorhexidine digluconate (CHX) for its antibacterial activity, drug release capacity, and physicochemical properties.

Methods

Drug release of CHX liposomal formulations in combination with BR was evaluated spectrophotometrically and through mathematical release models for 30 days. A selected combination was evaluated for antimicrobial properties against Enterococcus faecalis biofilm growth on human dentin. Cytotoxicity was assessed following the ISO 10993-5:2019 standard on days 1, 3, and 7. Physicochemical properties were evaluated through setting time, Fourier transform infrared spectroscopy, solubility, contact angle, and film thickness.

Results

From BR, liposomal CHX released up to 7-fold higher CHX than CHX solution (P < .05), following a triphasic drug release pattern compared to the CHX solution, which followed a quasi-Fickian diffusion. BR combined with a selected liposomal CHX completely inhibited E. faecalis biofilm growth compared to the combination of BR with CHX solution and the control group (P < .05). Liposomal CHX decreased the contact angle (P < .05) and solubility but increased cytotoxicity (P < .05) of BR, staying above the ISO threshold. None of the other physicochemical characteristics tested differed from BR (P > .05).

Conclusion

This liposomal formulation improved CHX release from BR, enhancing the antibacterial effectiveness. It presents a promising approach for local antibiofilm therapy in endodontics without substantially altering the physicochemical characteristics of BR.

简介:本研究旨在评估含有二葡萄糖酸氯己定(CHX)脂质体的 BioRoot RCS(BR)的抗菌活性、药物释放能力和理化性质:方法:通过分光光度法和数学释放模型对 CHX 脂质体制剂与 BR 组合的药物释放进行了为期 30 天的评估。评估了所选组合对人牙本质上粪肠球菌(E. faecalis)生物膜生长的抗菌特性。根据 ISO 10993-5:2019 标准,在第 1、3 和 7 天对细胞毒性进行了评估。通过凝固时间、傅立叶变换红外光谱(FTIR)、溶解度、接触角和薄膜厚度对理化性质进行了评估:结果:从 BR 中,脂质体 CHX 的释放量是 CHX 溶液的 7 倍(p0.05):结论:这种脂质体配方改善了 CHX 从 BR 中的释放,提高了抗菌效果。结论:这种脂质体制剂提高了 CHX 从 BR 中的释放率,增强了抗菌效果,为根管治疗中的局部抗生物膜疗法提供了一种很有前景的方法,而且不会大幅改变 BR 的理化特性。
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引用次数: 0
Guidelines for Authors 作者指南
IF 3.5 2区 医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE Pub Date : 2024-08-01 DOI: 10.1016/S0099-2399(24)00370-4
{"title":"Guidelines for Authors","authors":"","doi":"10.1016/S0099-2399(24)00370-4","DOIUrl":"10.1016/S0099-2399(24)00370-4","url":null,"abstract":"","PeriodicalId":15703,"journal":{"name":"Journal of endodontics","volume":"50 8","pages":"Pages A12, A14, A16, A18, A20-A21"},"PeriodicalIF":3.5,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141979157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effect of Orthodontic Treatment on the Outcomes of Endodontically Treated Teeth: A Cone-Beam Computed Tomography Analysis 正畸治疗对根管治疗牙齿结果的影响:CBCT 分析。
IF 3.5 2区 医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE Pub Date : 2024-08-01 DOI: 10.1016/j.joen.2024.05.002

Introduction

Often there is the need of moving endodontically treated teeth. Orthodontic movement may have no effect on the prognosis of teeth with root canal treatment (RCT). To verify this subject, we evaluated the effect of orthodontic movement on the prognosis of RCT teeth using cone-beam computed tomography (CBCT) and further explored the influence of orthodontic movement on the prognosis of RCT teeth with and without apical periodontitis (AP).

Methods

This retrospective study was conducted by evaluating 169 RCT teeth of 100 patients who had undergone fixed orthodontic treatment. AP was assessed and classified using the CBCT periapical index. Univariate analysis of RCT outcome was performed for the total RCT group, RCT without AP group and RCT with AP group. Multivariate logistic regression was performed for the total RCT group and RCT without AP group, respectively, but not for the RCT with AP group. Variables related to the prognosis of RCT were included, such as age, gender, tooth position, RCT quality, coronal restoration quality, periodontal condition, orthodontic traction distance, and orthodontic rotation angle.

Results

The orthodontic traction distance and rotation angle were not significantly correlated to the RCT outcomes, regardless of the presence of AP. Among the total RCT group, teeth with unqualified RCT (odds ratio = 3.42, P = .004) and inadequate coronal restoration (odds ratio = 4.40, P = .031) had a lower success rate. Of the 97 RCT teeth without AP, unqualified RCT was a risk factor for treatment failure (odds ratio = 3.55, P = .041). Of the 72 RCT teeth with AP, the univariate analysis showed that RCT quality were significantly related to the outcome (P = .042).

Conclusions

Orthodontic movement had no effect on the prognosis of RCT teeth regardless of the presence of AP.

导言:目的:根管治疗后的牙齿经常需要移动。正畸移动可能对接受根管治疗(RCT)的牙齿的预后没有影响。为了验证这一观点,我们使用锥束计算机断层扫描(CBCT)评估了正畸移动对根管治疗牙齿预后的影响,并进一步探讨了正畸移动对有和没有根尖牙周炎(AP)的根管治疗牙齿预后的影响:这项回顾性研究对 100 名接受过固定正畸治疗的患者的 169 颗 RCT 牙齿进行了评估。使用 CBCT 根尖周炎指数对根尖周炎进行评估和分类。对全部 RCT 组、无 AP RCT 组和有 AP RCT 组的 RCT 结果进行了单变量分析。分别对全部 RCT 组和无 AP RCT 组进行了多变量逻辑回归分析,但未对有 AP 的 RCT 组进行多变量逻辑回归分析。与RCT预后相关的变量包括年龄、性别、牙齿位置、RCT质量、冠状修复质量、牙周状况、正畸牵引距离和正畸旋转角度:结果:无论是否存在 AP,正畸牵引距离和正畸旋转角度与 RCT 结果无明显相关性。在所有 RCT 组中,RCT 不合格(OR = 3.42,P = 0.004)和冠状修复不充分(OR = 4.40,P = 0.031)的牙齿成功率较低。在 97 个没有 AP 的 RCT 牙齿中,不合格的 RCT 是治疗失败的风险因素(OR = 3.55,P = 0.041)。单变量分析表明,在 72 个有 AP 的 RCT 牙齿中,RCT 质量与治疗结果显著相关(P = 0.042):结论:无论是否存在 AP,正畸移动对 RCT 牙齿的预后都没有影响。
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引用次数: 0
Pulp Survival of Cracked Teeth with Reversible Pulpitis after Orthodontic Banding and Coronal Coverage – A Prospective Cohort Study with One Year Follow Up 正畸带环和冠状覆盖后可逆性牙髓炎裂纹牙的牙髓存活率 - 一项为期一年的前瞻性队列研究。
IF 3.5 2区 医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE Pub Date : 2024-08-01 DOI: 10.1016/j.joen.2024.05.006

Introduction

The aim of this prospective study was to investigate the 1-year pulp survival of cracked teeth with reversible pulpitis managed with initial stabilization using orthodontic bands, followed by coronal coverage restorations.

Methods

One-hundred-and-twenty-five patients with a cracked tooth with reversible pulpitis each were recruited. Preoperative patient and tooth data were collected. After definitive pulp diagnoses were determined following an interim period of orthodontic banding, coronal coverage restorations were placed. Cox and logistic regression analyses were used to assess possible prognostic factors and to correlate initial time to pulp stabilization while in orthodontic bands with eventual outcome. Pulp survival was determined using both clinical and radiographic findings.

Results

One-hundred-and-six cracked teeth were followed up at 1 year. Pulp survival based on clinical and radiographic findings was found in 81 teeth (76.4%). Out of 25 failures, 11 (44%) required root canal treatment (RCT) in the orthodontic band stage and 10 (40%) required RCT during the process of or after coronal coverage restorations. Four teeth (16%) had incidental findings of periapical radiolucencies at the 1-year review without clinical symptoms. Teeth requiring RCT were found to have required longer periods in orthodontic bands prior to a definitive pulp diagnosis (P < .05).

Conclusion

A step-by-step approach by using orthodontic banding to monitor pulp status may reduce the incidence of RCT required through definitive coronal coverage restorations for cracked teeth with reversible pulpitis.

导言:这项前瞻性研究的目的是调查患有可逆性牙髓炎的裂缝牙齿在使用正畸带进行初步稳定后,再进行冠状覆盖修复的1年牙髓存活情况:这项前瞻性研究的目的是调查患有可逆性牙髓炎的裂隙牙在使用正畸带进行初步稳定后,再进行冠状覆盖修复的1年牙髓存活率:方法:共招募了 125 名患有可逆性牙髓炎的裂隙牙患者。收集术前患者和牙齿数据。在正畸带中期确定牙髓诊断后,进行冠状覆盖修复。采用 Cox 和逻辑回归分析评估可能的预后因素,并将正畸带环期间牙髓稳定的初始时间与最终结果联系起来。牙髓存活率是通过临床和放射学检查结果确定的:对 166 颗裂开的牙齿进行了为期 1 年的随访。根据临床和放射学检查结果,发现有 81 颗牙齿(76.4%)的牙髓存活。在 25 颗失败的牙齿中,11 颗(44%)需要在正畸带阶段进行根管治疗,10 颗(40%)需要在冠状覆盖修复过程中或修复后进行根管治疗。有四颗牙齿(16%)在一年复查时偶然发现根尖周有放射线,但没有临床症状。需要进行RCT检查的牙齿在明确牙髓诊断之前需要较长的正畸带(p结论:通过使用正畸带监测牙髓状态的逐步方法,可以减少对患有可逆性牙髓炎的裂隙牙进行最终冠覆盖修复时所需要的RCT的发生率。
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引用次数: 0
Dynamic Photoelastic Analysis of Stress Distribution in Simulated Canals Using Rotary Instruments with Varied Tip and Taper Sizes: A Quasi-3D Approach 使用不同尖端和锥度的旋转仪器对模拟运河中的应力分布进行动态光弹性分析:准三维方法
IF 3.5 2区 医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE Pub Date : 2024-08-01 DOI: 10.1016/j.joen.2024.05.008

Introduction

To compare the stress produced on the walls of simulated canals by rotary instruments with varied tip and taper sizes.

Methods

Ninety isotropic transparent blocks, each containing a 60-degree curved canal, were distributed into 18 groups (n = 5) based on the instrument tip (sizes 10, 15, 20, 25, 30, and 35) and taper (sizes 0.02, 0.04, and 0.06). The blocks were fixed in a circular polariscope setup for dark field analysis. A digital camera was employed to capture the real-time birefringence patterns generated by each instrument. Digital image frames, corresponding to the instrument reaching the end of each canal third, were extracted and evaluated by 2 independent observers for the stress generation on canal walls. The data analysis employed a semi-quantitative scale ranging from 0 to 5. Cohen's Kappa coefficient test was used to determine the inter-observer agreement while the results were compared using Kruskal–Wallis test followed by an all-pairwise posthoc procedure (α = 5%).

Results

Inter-observer agreement was 0.95. A significant influence of the tip size on stress was observed across the coronal (P = .011), middle (P = .006), and apical (P = .026) thirds. In contrast, taper size did not affect the stress induced at the coronal (P = .509), middle (P = .958), or apical (P = .493) thirds. The variations in tip and taper sizes did not result in a significant stress differences among the thirds (P = .181).

Conclusions

The stress significantly increased across all canal thirds with larger tip sizes of rotary instruments, whereas the taper sizes did not influence the stress when compared to the canal thirds.

方法将九十个各向同性的透明块(每个块包含一个 60 度弯曲的牙槽骨)根据器械尖端(10、15、20、25、30 和 35 号)和锥度(0.02、0.04 和 0.06 号)分成 18 组(n = 5)。色块被固定在一个圆形偏振镜装置中进行暗场分析。数码相机用于捕捉每个仪器产生的实时双折射模式。提取仪器到达每三分之一管壁末端时的数字图像帧,并由两名独立观察者对管壁上产生的应力进行评估。数据分析采用 0 至 5 的半定量量表。结果观察者之间的一致性为 0.95。在冠状(P = .011)、中间(P = .006)和顶端(P = .026)三部分观察到牙尖大小对应力有明显影响。相反,锥度大小对冠状面(P = .509)、中部(P = .958)和顶端(P = .493)三部分的应力没有影响。结论旋转器械的尖端尺寸越大,所有三通管的应力都会显著增加,而锥体尺寸与三通管相比对应力没有影响。
{"title":"Dynamic Photoelastic Analysis of Stress Distribution in Simulated Canals Using Rotary Instruments with Varied Tip and Taper Sizes: A Quasi-3D Approach","authors":"","doi":"10.1016/j.joen.2024.05.008","DOIUrl":"10.1016/j.joen.2024.05.008","url":null,"abstract":"<div><h3>Introduction</h3><p>To compare the stress produced on the walls of simulated canals by rotary instruments with varied tip and taper sizes.</p></div><div><h3>Methods</h3><p>Ninety isotropic transparent blocks, each containing a 60-degree curved canal, were distributed into 18 groups (<em>n</em><span> = 5) based on the instrument tip (sizes 10, 15, 20, 25, 30, and 35) and taper (sizes 0.02, 0.04, and 0.06). The blocks were fixed in a circular polariscope setup for dark field analysis. A digital camera was employed to capture the real-time birefringence<span> patterns generated by each instrument. Digital image frames, corresponding to the instrument reaching the end of each canal third, were extracted and evaluated by 2 independent observers for the stress generation on canal walls. The data analysis employed a semi-quantitative scale ranging from 0 to 5. Cohen's Kappa coefficient test was used to determine the inter-observer agreement while the results were compared using Kruskal–Wallis test followed by an all-pairwise posthoc procedure (α = 5%).</span></span></p></div><div><h3>Results</h3><p>Inter-observer agreement was 0.95. A significant influence of the tip size on stress was observed across the coronal (<em>P</em> = .011), middle (<em>P</em> = .006), and apical (<em>P</em> = .026) thirds. In contrast, taper size did not affect the stress induced at the coronal (<em>P</em> = .509), middle (<em>P</em> = .958), or apical (<em>P</em> = .493) thirds. The variations in tip and taper sizes did not result in a significant stress differences among the thirds (<em>P</em> = .181).</p></div><div><h3>Conclusions</h3><p>The stress significantly increased across all canal thirds with larger tip sizes of rotary instruments, whereas the taper sizes did not influence the stress when compared to the canal thirds.</p></div>","PeriodicalId":15703,"journal":{"name":"Journal of endodontics","volume":"50 8","pages":"Pages 1151-1158"},"PeriodicalIF":3.5,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141130335","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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Journal of endodontics
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