Hellena D. Jacobo, Petro E. Mabeyo, Aldo J. Kitalika
Pesticide residues in staple foods pose significant public health concerns, especially in regions with intensive agrochemical use and weak regulatory enforcement. This study examined the presence and reduction of pesticide residues in 75 maize grain samples and their processed products collected from five milling factories in Nyamagana and Ilemela districts, Mwanza Region, Tanzania. Residue extraction was performed using solid–liquid and liquid–liquid methods, followed by quantification via gas chromatography–tandem mass spectrometry (GC-MS/MS). Sixteen pesticide compounds were identified, including malathion, pirimiphos-methyl, p,p ′-DDT, p,p ′-DDE, and trichlorfon. Two postharvest treatments, washing (single washing and double washing) and debranning, achieved average residue reductions of 32.92% ± 11.37%, 66.74% ± 11.62%, and 99.32% ± 0.32%, respectively. Combined washing and debranning removed detectable residues in polished (sembe) flour. Residue distribution analysis revealed higher concentrations in the outer layer, germ, and tip regions of the grain, underscoring the importance of debranning as a key intervention, despite the associated nutrient loss. However, bran from unwashed maize retained elevated residue levels, risking bioconcentration when used as animal feed. These results underscore the importance of standardized processing protocols and stricter pesticide regulations to enhance food safety and safeguard public health.
{"title":"Assessment of Water-Based Treatments and Debranning Methods for Removal of Pesticide Residues in Maize Grains and Their Products","authors":"Hellena D. Jacobo, Petro E. Mabeyo, Aldo J. Kitalika","doi":"10.1155/jfpp/5502432","DOIUrl":"https://doi.org/10.1155/jfpp/5502432","url":null,"abstract":"<p>Pesticide residues in staple foods pose significant public health concerns, especially in regions with intensive agrochemical use and weak regulatory enforcement. This study examined the presence and reduction of pesticide residues in 75 maize grain samples and their processed products collected from five milling factories in Nyamagana and Ilemela districts, Mwanza Region, Tanzania. Residue extraction was performed using solid–liquid and liquid–liquid methods, followed by quantification via gas chromatography–tandem mass spectrometry (GC-MS/MS). Sixteen pesticide compounds were identified, including malathion, pirimiphos-methyl, p,p <sup>′</sup>-DDT, p,p <sup>′</sup>-DDE, and trichlorfon. Two postharvest treatments, washing (single washing and double washing) and debranning, achieved average residue reductions of 32.92<i>%</i> ± 11.37<i>%</i>, 66.74<i>%</i> ± 11.62<i>%</i>, and 99.32<i>%</i> ± 0.32<i>%</i>, respectively. Combined washing and debranning removed detectable residues in polished (<i>sembe</i>) flour. Residue distribution analysis revealed higher concentrations in the outer layer, germ, and tip regions of the grain, underscoring the importance of debranning as a key intervention, despite the associated nutrient loss. However, bran from unwashed maize retained elevated residue levels, risking bioconcentration when used as animal feed. These results underscore the importance of standardized processing protocols and stricter pesticide regulations to enhance food safety and safeguard public health.</p>","PeriodicalId":15717,"journal":{"name":"Journal of Food Processing and Preservation","volume":"2025 1","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jfpp/5502432","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145824900","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This study focused on the development and characterization of zein–pectin nanoparticles designed to encapsulate tanshinones, which are natural antioxidants found in the roots of Salvia subg. Perovskia. The researchers aimed to evaluate the effects of these nanoparticles on apple juice. T/Z/P NPs were added to the apple juice at concentrations of 200–400 ppm, and the physicochemical stability, antioxidant capacity, color, and turbidity of the fortified juice were monitored at 4°C over a period of 30 days. The results showed that apple juice enriched with 200 ppm of nanoparticles exhibited better stability, with particle sizes measuring 0.16 ± 0.00 μm and zeta potential remaining consistently below –30 mV. Additionally, there was a retention of 77.32% ± 0.12% of the tanshinones. The encapsulation process significantly increased both the total phenolic content and antioxidant capacity of the juice. Although the addition of nanoparticles led to a notable increase in turbidity, the change in color of the apple juice was minimal. Overall, the findings indicate that pectinate nanocarriers can effectively preserve tanshinones and enhance the functional properties of apple juice. However, the study also highlights the importance of considering visual characteristics when formulating beverages.
{"title":"Fortification of Apple Juice With Tanshinone-Loaded Pectin–Zein Nanoparticles for Enhanced Nutritional and Antioxidant Attributes","authors":"Ameneh Elmizadeh, Sayed Amir Hossein Goli","doi":"10.1155/jfpp/1254027","DOIUrl":"https://doi.org/10.1155/jfpp/1254027","url":null,"abstract":"<p>This study focused on the development and characterization of zein–pectin nanoparticles designed to encapsulate tanshinones, which are natural antioxidants found in the roots of <i>Salvia subg. Perovskia</i>. The researchers aimed to evaluate the effects of these nanoparticles on apple juice. T/Z/P NPs were added to the apple juice at concentrations of 200–400 ppm, and the physicochemical stability, antioxidant capacity, color, and turbidity of the fortified juice were monitored at 4°C over a period of 30 days. The results showed that apple juice enriched with 200 ppm of nanoparticles exhibited better stability, with particle sizes measuring 0.16 ± 0.00 <i>μ</i>m and zeta potential remaining consistently below –30 mV. Additionally, there was a retention of 77.32<i>%</i> ± 0.12<i>%</i> of the tanshinones. The encapsulation process significantly increased both the total phenolic content and antioxidant capacity of the juice. Although the addition of nanoparticles led to a notable increase in turbidity, the change in color of the apple juice was minimal. Overall, the findings indicate that pectinate nanocarriers can effectively preserve tanshinones and enhance the functional properties of apple juice. However, the study also highlights the importance of considering visual characteristics when formulating beverages.</p>","PeriodicalId":15717,"journal":{"name":"Journal of Food Processing and Preservation","volume":"2025 1","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jfpp/1254027","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145824872","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Radovan Čobanović, Sunčica Kocić-Tanackov, Slobodan Milošević, Ivana Čabarkapa, Bojana Kokić, Milan Vukić, Dejan Maletić
In this study, PAW was generated using an argon plasma jet in the presence and absence of magnesium plates as a source of Mg2+ ions. The physicochemical properties of PAW were characterized by measuring pH, oxidation-reduction potential, electrical conductivity, and the concentrations of H₂O₂, NO₂−, and NO₃− during generation and subsequent storage. Results demonstrated that PAW without Mg exhibited higher ORP values (≈420 mV) and lower pH (5.3–5.7), while PAW Mg maintained a near-neutral pH (6.2–6.4) and significantly lower ORP (≈75–275 mV). Hydrogen peroxide levels were generally higher in PAW without Mg during most of the generation period (10–50 min), whereas PAW Mg retained slightly higher concentrations at the 60-min endpoint, indicating a stabilizing effect of magnesium on reactive species. PAW Mg also showed slower degradation of RONS during the aging period, confirming its superior stability. The antimicrobial activity of both PAW types was evaluated against six planktonic bacterial strains, including Gram-negative (Escherichia coli O157:H7, Salmonella enteritidis, Pseudomonas aeruginosa) and Gram-positive (Staphylococcus aureus, Listeria monocytogenes, Bacillus cereus). PAW Mg achieved total inactivation (≈8 log₁₀ reduction) of all species within 24 h, while PAW without Mg also demonstrated strong bactericidal efficacy, with reductions above 5 log₁₀ for all tested bacteria after 24 h. Gram-positive strains were generally more resistant, with B. cereus showing the lowest susceptibility. Scanning electron microscopy revealed pronounced membrane damage and morphological alterations, particularly in cells treated with PAW Mg, consistent with the higher bactericidal efficiency of this treatment. Overall, Mg2+ incorporation reduced ORP but stabilized reactive species, extending antimicrobial activity and improving efficacy. PAW Mg emerged as a more stable disinfectant, while PAW without Mg also showed strong microbial inactivation, supporting their potential in food safety and environmental use.
{"title":"Effect of the Inclusion of Magnesium Ions During Generation Process of Plasma-Activated Water on Disinfectant Efficacy and Sustainability","authors":"Radovan Čobanović, Sunčica Kocić-Tanackov, Slobodan Milošević, Ivana Čabarkapa, Bojana Kokić, Milan Vukić, Dejan Maletić","doi":"10.1155/jfpp/5342852","DOIUrl":"https://doi.org/10.1155/jfpp/5342852","url":null,"abstract":"<p>In this study, PAW was generated using an argon plasma jet in the presence and absence of magnesium plates as a source of Mg<sup>2+</sup> ions. The physicochemical properties of PAW were characterized by measuring pH, oxidation-reduction potential, electrical conductivity, and the concentrations of H₂O₂, NO₂<sup>−</sup>, and NO₃<sup>−</sup> during generation and subsequent storage. Results demonstrated that PAW without Mg exhibited higher OR<i>P</i> values (≈420 mV) and lower pH (5.3–5.7), while PAW Mg maintained a near-neutral pH (6.2–6.4) and significantly lower ORP (≈75–275 mV). Hydrogen peroxide levels were generally higher in PAW without Mg during most of the generation period (10–50 min), whereas PAW Mg retained slightly higher concentrations at the 60-min endpoint, indicating a stabilizing effect of magnesium on reactive species. PAW Mg also showed slower degradation of RONS during the aging period, confirming its superior stability. The antimicrobial activity of both PAW types was evaluated against six planktonic bacterial strains, including Gram-negative (<i>Escherichia coli</i> O157:H7, <i>Salmonella enteritidis</i>, <i>Pseudomonas aeruginosa</i>) and Gram-positive (<i>Staphylococcus aureus</i>, <i>Listeria monocytogenes</i>, <i>Bacillus cereus</i>). PAW Mg achieved total inactivation (≈8 log₁₀ reduction) of all species within 24 h, while PAW without Mg also demonstrated strong bactericidal efficacy, with reductions above 5 log₁₀ for all tested bacteria after 24 h. Gram-positive strains were generally more resistant, with <i>B. cereus</i> showing the lowest susceptibility. Scanning electron microscopy revealed pronounced membrane damage and morphological alterations, particularly in cells treated with PAW Mg, consistent with the higher bactericidal efficiency of this treatment. Overall, Mg<sup>2+</sup> incorporation reduced ORP but stabilized reactive species, extending antimicrobial activity and improving efficacy. PAW Mg emerged as a more stable disinfectant, while PAW without Mg also showed strong microbial inactivation, supporting their potential in food safety and environmental use.</p>","PeriodicalId":15717,"journal":{"name":"Journal of Food Processing and Preservation","volume":"2025 1","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jfpp/5342852","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145845788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The study aims to enrich a mango-based beverage by incorporating basil seed gum (BSG), leveraging its strong antioxidant and nutritional properties. Mango pulp and basil seed were analyzed, with BSG extracted and added to beverages at 0%–0.3% concentrations. Samples underwent sensory and chemical evaluation, with results statistically analyzed for significance. The mango beverage with 0.1% BSG had the highest sensory scores and improved chemical and antioxidant profiles. Specifically, Sample B (0.1% BSG) showed higher antioxidant activity %RSA (49.16%), total phenolic content (TPC) (568.892 μg GAE/mL), total flavonoid content (TFC) (86.790 μg GAE/mL), ascorbic acid (6.537 mg/100 g), and greater sedimentation stability (indicating the hydrocolloid impact of BSG) compared to the control sample (A). In conclusion, incorporating 0.1% BSG significantly improved the beverage’s sensory and nutritional qualities, underscoring its promise as a natural functional ingredient and supporting the consumerization of basil seed beverages as nutritionally enriched, stable functional drinks.
{"title":"Development and Enrichment of a Mango-Based Beverage Using Basil Seed Gum as a Functional Hydrocolloid","authors":"Rabina B. K., Babita Adhikari, Ganga Sangroula","doi":"10.1155/jfpp/7144975","DOIUrl":"https://doi.org/10.1155/jfpp/7144975","url":null,"abstract":"<p>The study aims to enrich a mango-based beverage by incorporating basil seed gum (BSG), leveraging its strong antioxidant and nutritional properties. Mango pulp and basil seed were analyzed, with BSG extracted and added to beverages at 0%–0.3% concentrations. Samples underwent sensory and chemical evaluation, with results statistically analyzed for significance. The mango beverage with 0.1% BSG had the highest sensory scores and improved chemical and antioxidant profiles. Specifically, Sample B (0.1% BSG) showed higher antioxidant activity %RSA (49.16%), total phenolic content (TPC) (568.892 <i>μ</i>g GAE/mL), total flavonoid content (TFC) (86.790 <i>μ</i>g GAE/mL), ascorbic acid (6.537 mg/100 g), and greater sedimentation stability (indicating the hydrocolloid impact of BSG) compared to the control sample (A). In conclusion, incorporating 0.1% BSG significantly improved the beverage’s sensory and nutritional qualities, underscoring its promise as a natural functional ingredient and supporting the consumerization of basil seed beverages as nutritionally enriched, stable functional drinks.</p>","PeriodicalId":15717,"journal":{"name":"Journal of Food Processing and Preservation","volume":"2025 1","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jfpp/7144975","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145848062","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Moringa oleifera is a plant of immense nutritional and medicinal value, particularly when cultivated organically. It is native to the Indian subcontinent and widely cultivated in tropical regions. Known as the “miracle tree,” it is used in various industries, from food to pharmaceuticals, due to its rich bioactive compounds and antioxidant properties. This study investigates the phytochemical content, nutritional properties, and antioxidant capabilities of organic M. oleifera leaves under various drying conditions (sun, oven, and room drying [RD]). The observed changes suggest that temperature has a significant impact on the biochemical constituents, such as proteins and vitamins, through denaturation or degradation. In addition, the RD and control samples exhibited similar concentrations of Vitamin E (11.837 ± 1.387 and 11.621 ± 3.320, respectively). Both sun drying (7.512 ± 0.350) and oven drying (7.550 ± 1.547) resulted in a decrease in Vitamin E content. However, the Fourier transform infrared (FTIR) spectroscopy analysis of functional groups revealed consistent peaks across all samples, indicating the presence of polysaccharides (1039–1042 cm−1, C–O stretch), proteins (1233–1235 cm−1, C–N stretch; 1541–1542 cm−1, amide II; 1642 cm−1, amide I), lipids (1732–1733 cm−1, ester C=O), and aliphatic compounds (2844–2912 cm−1, C–H stretch), demonstrating that the core biochemical composition remained stable regardless of drying method. The drying methods for the organic M. oleifera revealed significant differences (p < 0.05) in antioxidant activities (metal chelating activity, 2,2-diphenyl-1-picrylhydrazyl [DPPH], and hydroxyl scavenging activity) among the samples, with oven drying having the most damaging effect. Additionally, the RD sample at the lower concentration showed the highest ferric-reducing power, with an absorbance of 0.759 at 700 nm. The results of the antioxidant test corroborate with the variation of better values in RD than sun or oven drying. These findings support the diverse applications of M. oleifera in nutrition, medicine, and cosmetics.
{"title":"Effect of Drying Methods on Nutritional and Antioxidant Properties of Organic Moringa oleifera Leaves From the Sahel","authors":"Massaoudou Mahamane, Issoufou Amadou, Mahamadou Rabiou Moudi Aboubacar","doi":"10.1155/jfpp/1994240","DOIUrl":"https://doi.org/10.1155/jfpp/1994240","url":null,"abstract":"<p><i>Moringa oleifera</i> is a plant of immense nutritional and medicinal value, particularly when cultivated organically. It is native to the Indian subcontinent and widely cultivated in tropical regions. Known as the “miracle tree,” it is used in various industries, from food to pharmaceuticals, due to its rich bioactive compounds and antioxidant properties. This study investigates the phytochemical content, nutritional properties, and antioxidant capabilities of organic <i>M. oleifera</i> leaves under various drying conditions (sun, oven, and room drying [RD]). The observed changes suggest that temperature has a significant impact on the biochemical constituents, such as proteins and vitamins, through denaturation or degradation. In addition, the RD and control samples exhibited similar concentrations of Vitamin E (11.837 ± 1.387 and 11.621 ± 3.320, respectively). Both sun drying (7.512 ± 0.350) and oven drying (7.550 ± 1.547) resulted in a decrease in Vitamin E content. However, the Fourier transform infrared (FTIR) spectroscopy analysis of functional groups revealed consistent peaks across all samples, indicating the presence of polysaccharides (1039–1042 cm<sup>−1</sup>, C–O stretch), proteins (1233–1235 cm<sup>−1</sup>, C–N stretch; 1541–1542 cm<sup>−1</sup>, amide II; 1642 cm<sup>−1</sup>, amide I), lipids (1732–1733 cm<sup>−1</sup>, ester C=O), and aliphatic compounds (2844–2912 cm<sup>−1</sup>, C–H stretch), demonstrating that the core biochemical composition remained stable regardless of drying method. The drying methods for the organic <i>M. oleifera</i> revealed significant differences (<i>p</i> < 0.05) in antioxidant activities (metal chelating activity, 2,2-diphenyl-1-picrylhydrazyl [DPPH], and hydroxyl scavenging activity) among the samples, with oven drying having the most damaging effect. Additionally, the RD sample at the lower concentration showed the highest ferric-reducing power, with an absorbance of 0.759 at 700 nm. The results of the antioxidant test corroborate with the variation of better values in RD than sun or oven drying. These findings support the diverse applications of <i>M. oleifera</i> in nutrition, medicine, and cosmetics.</p>","PeriodicalId":15717,"journal":{"name":"Journal of Food Processing and Preservation","volume":"2025 1","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jfpp/1994240","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145695027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In this study, changes in the quality characteristics of carrots, which are increasingly being used for processing applications, during blanching by three different methods, namely, hot water (HW), microwave (MW), and combined hot water and microwave (HW-MW) treatments, were compared. Blanching time, as measured by residual peroxidase (POD) activity, was reduced to one-third for the MW and HW-MW treatments (40 s) compared with the HW treatment (120 s). During MW blanching, tissue damage was observed due to heating from the center of the sample and a rapid increase in internal pressure, whereas HW-MW blanching did not damage the sample because heating proceeded from outside the sample. Compared with the other treatments, the short processing time resulted in a greater percentage (71%–78%) of L-ascorbic acid remaining in the MW and HW-MW treatments, and particularly in the HW-MW treatment, quality deterioration, such as weight loss and color change, tended to be suppressed. The MW treatment caused significant tissue softening; however, the initial firmness was maintained during the HW-MW treatment because of the suppression of structural cell wall fracture. These results suggest that the use of HW-MW is an effective method for blanching carrots in terms of reducing time and quality loss during treatment.
{"title":"Evaluation of the Quality Characteristics of Carrots in Combination With Hot Water and Microwave Blanching","authors":"Takahiro Orikasa, Yasumasa Ando, Natsuki Okoshi, Ryo Akahira, Kanoko Miura, Misaki Komuro, Kuniaki Sasaki, Shoji Koide","doi":"10.1155/jfpp/7691678","DOIUrl":"https://doi.org/10.1155/jfpp/7691678","url":null,"abstract":"<p>In this study, changes in the quality characteristics of carrots, which are increasingly being used for processing applications, during blanching by three different methods, namely, hot water (HW), microwave (MW), and combined hot water and microwave (HW-MW) treatments, were compared. Blanching time, as measured by residual peroxidase (POD) activity, was reduced to one-third for the MW and HW-MW treatments (40 s) compared with the HW treatment (120 s). During MW blanching, tissue damage was observed due to heating from the center of the sample and a rapid increase in internal pressure, whereas HW-MW blanching did not damage the sample because heating proceeded from outside the sample. Compared with the other treatments, the short processing time resulted in a greater percentage (71%–78%) of L-ascorbic acid remaining in the MW and HW-MW treatments, and particularly in the HW-MW treatment, quality deterioration, such as weight loss and color change, tended to be suppressed. The MW treatment caused significant tissue softening; however, the initial firmness was maintained during the HW-MW treatment because of the suppression of structural cell wall fracture. These results suggest that the use of HW-MW is an effective method for blanching carrots in terms of reducing time and quality loss during treatment.</p>","PeriodicalId":15717,"journal":{"name":"Journal of Food Processing and Preservation","volume":"2025 1","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jfpp/7691678","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145695026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Asghar Farhadi, Ali Ayaseh, Babak Ghanbarzadeh, Hamed Hamishehkar
This study focused on producing hydrolysates from Sargassum macroalgae as a novel and sustainable protein source, aimed at developing an eco-friendly alternative to animal and plant-based proteins. The research evaluated the effects of enzymatic hydrolysis using Alcalase, pancreatin, trypsin, and pepsin on the technofunctional properties, nutritional value, and antioxidant and antimicrobial properties of Sargassum protein (SP) and Sargassum protein hydrolysates (SPHs). The protein extracted from Sargassum was subjected to enzymatic hydrolysis under optimal conditions for various proteases, and the degree of hydrolysis (DH) was evaluated. Trypsin exhibited the lowest DH (19.3%), revealing variations in enzyme efficiency, while pancreatin demonstrated the highest (23.8%). From a nutritional standpoint, analysis of the amino acid composition (AAC) of SP and SPHs demonstrated significant levels of essential amino acids (EAAs) at 43% and antioxidant amino acids (AAAs) at 16%, accompanied by a protein efficiency ratio (PER) of 2.17. From a technological perspective, enzymatic hydrolysis enhanced the solubility, emulsifying properties, and foaming ability of SP. Enzymatic hydrolysis of SP significantly enhanced antioxidant properties, as demonstrated by DPPH (80.6%), ABTS (84.0%), hydroxyl radical (83.6%), and nitric oxide (34.3%) scavenging activities. Total antioxidant activity, measured using the phosphomolybdenum method, reached a peak absorbance of 1.11 at 695 nm, indicating strong reducing power. Furthermore, notable improvements were observed in ferrous and copper ion–chelating activities. Peptides produced by Alcalase and pancreatin exhibited the greatest inhibition of Escherichia coli (13.9 mm) and Staphylococcus aureus (11.3 mm). These findings indicate that peptides from Sargassum macroalgae have the potential to be valuable nutrients and natural preservatives for food and health products.
{"title":"Production of Hydrolysates From Sargassum Protein: Evaluation of Technofunctional, Nutritional, Antioxidant, and Antimicrobial Properties","authors":"Asghar Farhadi, Ali Ayaseh, Babak Ghanbarzadeh, Hamed Hamishehkar","doi":"10.1155/jfpp/1996776","DOIUrl":"https://doi.org/10.1155/jfpp/1996776","url":null,"abstract":"<p>This study focused on producing hydrolysates from <i>Sargassum</i> macroalgae as a novel and sustainable protein source, aimed at developing an eco-friendly alternative to animal and plant-based proteins. The research evaluated the effects of enzymatic hydrolysis using Alcalase, pancreatin, trypsin, and pepsin on the technofunctional properties, nutritional value, and antioxidant and antimicrobial properties of <i>Sargassum</i> protein (SP) and <i>Sargassum</i> protein hydrolysates (SPHs). The protein extracted from <i>Sargassum</i> was subjected to enzymatic hydrolysis under optimal conditions for various proteases, and the degree of hydrolysis (DH) was evaluated. Trypsin exhibited the lowest DH (19.3%), revealing variations in enzyme efficiency, while pancreatin demonstrated the highest (23.8%). From a nutritional standpoint, analysis of the amino acid composition (AAC) of SP and SPHs demonstrated significant levels of essential amino acids (EAAs) at 43% and antioxidant amino acids (AAAs) at 16%, accompanied by a protein efficiency ratio (PER) of 2.17. From a technological perspective, enzymatic hydrolysis enhanced the solubility, emulsifying properties, and foaming ability of SP. Enzymatic hydrolysis of SP significantly enhanced antioxidant properties, as demonstrated by DPPH (80.6%), ABTS (84.0%), hydroxyl radical (83.6%), and nitric oxide (34.3%) scavenging activities. Total antioxidant activity, measured using the phosphomolybdenum method, reached a peak absorbance of 1.11 at 695 nm, indicating strong reducing power. Furthermore, notable improvements were observed in ferrous and copper ion–chelating activities. Peptides produced by Alcalase and pancreatin exhibited the greatest inhibition of <i>Escherichia coli</i> (13.9 mm) and <i>Staphylococcus aureus</i> (11.3 mm). These findings indicate that peptides from <i>Sargassum</i> macroalgae have the potential to be valuable nutrients and natural preservatives for food and health products.</p>","PeriodicalId":15717,"journal":{"name":"Journal of Food Processing and Preservation","volume":"2025 1","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jfpp/1996776","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145626254","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Thi Ngoc Nhon Hoang, Khanh Dieu Dinh, Thi Hong Anh Le, Thi Anh Dao Dong
Musa balbisiana inflorescence is an often overlooked part of the plant, but it has a great potential for bioactive compounds. This study is aimed at examining the impacts and optimize the extraction conditions of microwave-assisted extraction (MAE) for obtaining extract-enriched bioactive compounds, namely, polyphenols and saponins, from M. balbisiana inflorescence. Using the response surface methodology (RSM) with full factorial two-step experimental design and Box–Behnken model, the optimal MAE conditions were determined, including 70% methanol, microwave power 291.87 W, microwave irradiation cycle 4.28 s/min, and treatment time 45.33 min, resulting in total polyphenol content (TPC) and total saponin content (TSC) of 50.63 mgGAE/gDM and 59.15 mg/gDM, respectively. These results have significant agreement between predicted and experimental values, confirming the accuracy of the model. Additionally, structural examination with Raman and FT-IR spectroscopy exposed the functional group features of phenolic and saponin compounds. LC/MS spectra predicted several polyphenol and saponin compounds in the M. balbisiana inflorescence sample, including oleanolic acid, which was also presumed to be present in the purified fraction through NMR spectra. Raman (1657, 1458, and 1307 cm−1), FT-IR (3473.4, 2946.7, 1696.4, and 1092.5 cm−1), LC/MS ([M + H]+m/z 271.1, 457.3, and 649.4), and NMR analyses of M. balbisiana inflorescence extract revealed the presence of polyphenols (apigenin) and saponins (oleanolic acid and oleanolic acid glycoside), with the triterpenoid structure of oleanolic acid confirmed by NMR data.
{"title":"Microwave-Assisted Extraction of Bioactive Polyphenols and Saponins From Musa balbisiana Inflorescence: Optimization and Structural Analysis","authors":"Thi Ngoc Nhon Hoang, Khanh Dieu Dinh, Thi Hong Anh Le, Thi Anh Dao Dong","doi":"10.1155/jfpp/5922973","DOIUrl":"https://doi.org/10.1155/jfpp/5922973","url":null,"abstract":"<p><i>Musa balbisiana</i> inflorescence is an often overlooked part of the plant, but it has a great potential for bioactive compounds. This study is aimed at examining the impacts and optimize the extraction conditions of microwave-assisted extraction (MAE) for obtaining extract-enriched bioactive compounds, namely, polyphenols and saponins, from <i>M. balbisiana</i> inflorescence. Using the response surface methodology (RSM) with full factorial two-step experimental design and Box–Behnken model, the optimal MAE conditions were determined, including 70% methanol, microwave power 291.87 W, microwave irradiation cycle 4.28 s/min, and treatment time 45.33 min, resulting in total polyphenol content (TPC) and total saponin content (TSC) of 50.63 mgGAE/gDM and 59.15 mg/gDM, respectively. These results have significant agreement between predicted and experimental values, confirming the accuracy of the model. Additionally, structural examination with Raman and FT-IR spectroscopy exposed the functional group features of phenolic and saponin compounds. LC/MS spectra predicted several polyphenol and saponin compounds in the <i>M. balbisiana</i> inflorescence sample, including oleanolic acid, which was also presumed to be present in the purified fraction through NMR spectra. Raman (1657, 1458, and 1307 cm<sup>−1</sup>), FT-IR (3473.4, 2946.7, 1696.4, and 1092.5 cm<sup>−1</sup>), LC/MS ([M + H]<sup>+</sup><i>m</i>/<i>z</i> 271.1, 457.3, and 649.4), and NMR analyses of <i>M. balbisiana</i> inflorescence extract revealed the presence of polyphenols (apigenin) and saponins (oleanolic acid and oleanolic acid glycoside), with the triterpenoid structure of oleanolic acid confirmed by NMR data.</p>","PeriodicalId":15717,"journal":{"name":"Journal of Food Processing and Preservation","volume":"2025 1","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jfpp/5922973","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145626695","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Egg quality is a critical factor influencing consumer preference, nutrition, and industrial utilization, yet comparative evaluations of indigenous, synthetic, and exotic chicken breeds under uniform management are limited. This study assessed the physical, functional, and nutritional properties of eggs from Horro and Jarso (indigenous), White Feather (synthetic), and Koekoek (exotic) chickens reared under identical housing and feeding conditions. Forty eggs (10 per breed) were analyzed for color, density, porosity, flowability, protein, and mineral composition, with data evaluated using ANOVA and Tukey’s post hoc test (p < 0.05). Koekoek eggs showed the highest lightness and yellowness, while Horro eggs were darker but exhibited superior bulk and tapped density; Koekoek displayed the best flowability. Nutrient analysis revealed Koekoek eggs had the highest protein and copper contents, Jarso excelled in potassium, sodium, and manganese, and Horro contained the most calcium and iron, whereas Jarso eggshells were the richest in calcium and protein. These findings demonstrate significant breed-related differences in egg quality traits, underscoring the genetic basis of color, functionality, and mineral composition, and highlight opportunities for targeted breed utilization in poultry production, nutritional improvement, and egg powder applications.
鸡蛋质量是影响消费者偏好、营养和工业利用的关键因素,但对统一管理下的本地、合成和外来鸡品种的比较评价有限。本研究评估了在相同鸡舍和饲养条件下饲养的本土鸡Horro and Jarso、合成鸡White Feather和外来鸡Koekoek的鸡蛋的物理、功能和营养特性。对40只鸡蛋(每个品种10只)进行颜色、密度、孔隙度、流动性、蛋白质和矿物质成分分析,数据采用方差分析和Tukey事后检验(p < 0.05)进行评估。Koekoek的蛋亮度和黄度最高,Horro的蛋颜色较深,但体积和密度都较好;果胶的流动性最好。营养分析结果显示,鸭蛋的蛋白质和铜含量最高,鸭蛋的钾、钠和锰含量最高,鸭蛋的钙和铁含量最高,鸭蛋的钙和铁含量最高,鸭蛋的钙和蛋白质含量最高。这些发现表明了蛋品质性状的显著品种差异,强调了颜色、功能和矿物成分的遗传基础,并强调了在家禽生产、营养改善和蛋粉应用中有针对性地利用品种的机会。
{"title":"Evaluation of Egg Quality Differences Between Indigenous, Synthetic, and Exotic Chickens Under the Same Management","authors":"Dejenie Mengistie, Biadge Kefale, Betelhem Seyoum, Shelema Kelbessa Bora, Gebrerufael Girmay, Abdi Bedassa, Bezina Arega","doi":"10.1155/jfpp/7924659","DOIUrl":"https://doi.org/10.1155/jfpp/7924659","url":null,"abstract":"<p>Egg quality is a critical factor influencing consumer preference, nutrition, and industrial utilization, yet comparative evaluations of indigenous, synthetic, and exotic chicken breeds under uniform management are limited. This study assessed the physical, functional, and nutritional properties of eggs from Horro and Jarso (indigenous), White Feather (synthetic), and Koekoek (exotic) chickens reared under identical housing and feeding conditions. Forty eggs (10 per breed) were analyzed for color, density, porosity, flowability, protein, and mineral composition, with data evaluated using ANOVA and Tukey’s post hoc test (<i>p</i> < 0.05). Koekoek eggs showed the highest lightness and yellowness, while Horro eggs were darker but exhibited superior bulk and tapped density; Koekoek displayed the best flowability. Nutrient analysis revealed Koekoek eggs had the highest protein and copper contents, Jarso excelled in potassium, sodium, and manganese, and Horro contained the most calcium and iron, whereas Jarso eggshells were the richest in calcium and protein. These findings demonstrate significant breed-related differences in egg quality traits, underscoring the genetic basis of color, functionality, and mineral composition, and highlight opportunities for targeted breed utilization in poultry production, nutritional improvement, and egg powder applications.</p>","PeriodicalId":15717,"journal":{"name":"Journal of Food Processing and Preservation","volume":"2025 1","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jfpp/7924659","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145625908","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abu Bakker Siddique Khan, Mousumi Akhter, Zahidul Islam, Md. Aktaruzzaman, Mritunjoy Paul, Shafiqur Rahman, Anuradha Bhadra
This research was aimed at utilizing available resources and evaluating the effectiveness of value-added metrics for fish-based products, such as fish balls made from various types of fish mince and small tuna (Euthynnus affinis). The fish balls were stored using two distinct approaches: deep freezing (−18°C) for 60 days and refrigeration (4°C). The gustatory approach and the AOAC method were used to gauge the nutritional content and aesthetic effectiveness of the fish products, respectively. The deep-frozen samples exhibited improved crispness compared to those stored in the refrigerator. Deep-frozen fish balls had substantially lower pH, total volatile base-nitrogen (TVB-N), trimethylamine nitrogen (TMA-N), and histamine concentrations during the storage period (p < 0.05) than chilled fish balls. Salmonella species were not detected during the diagnostic process, while the Escherichia coli plate count in the deep-frozen fish balls significantly decreased to just under 105 cfu/g. Fish mince-based value-added seafood products could help Bangladesh utilize its resources more efficiently and contribute to maintaining sustainable food production. Furthermore, minimal adverse nutritional effects were observed, and the tuna fish balls remained safe for consumption after storage at −18°C for 60 days.
{"title":"Biochemical, Nutritional, and Microbiological Attributes of the Value-Added Fish Balls Derived From Little Tuna (Euthynnus affinis) in Perspective of Greater Utilization of Marine Cuisine in Bangladesh","authors":"Abu Bakker Siddique Khan, Mousumi Akhter, Zahidul Islam, Md. Aktaruzzaman, Mritunjoy Paul, Shafiqur Rahman, Anuradha Bhadra","doi":"10.1155/jfpp/8890059","DOIUrl":"https://doi.org/10.1155/jfpp/8890059","url":null,"abstract":"<p>This research was aimed at utilizing available resources and evaluating the effectiveness of value-added metrics for fish-based products, such as fish balls made from various types of fish mince and small tuna <i>(Euthynnus affinis).</i> The fish balls were stored using two distinct approaches: deep freezing (−18°C) for 60 days and refrigeration (4°C). The gustatory approach and the AOAC method were used to gauge the nutritional content and aesthetic effectiveness of the fish products, respectively. The deep-frozen samples exhibited improved crispness compared to those stored in the refrigerator. Deep-frozen fish balls had substantially lower pH, total volatile base-nitrogen (TVB-N), trimethylamine nitrogen (TMA-N), and histamine concentrations during the storage period (<i>p</i> < 0.05) than chilled fish balls. <i>Salmonella</i> species were not detected during the diagnostic process, while the <i>Escherichia coli</i> plate count in the deep-frozen fish balls significantly decreased to just under 10<sup>5</sup> cfu/g. Fish mince-based value-added seafood products could help Bangladesh utilize its resources more efficiently and contribute to maintaining sustainable food production. Furthermore, minimal adverse nutritional effects were observed, and the tuna fish balls remained safe for consumption after storage at −18°C for 60 days.</p>","PeriodicalId":15717,"journal":{"name":"Journal of Food Processing and Preservation","volume":"2025 1","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jfpp/8890059","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145581432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}