Pub Date : 2019-09-29DOI: 10.31491/csrc.2019.09.002
Kaidi Sun, Jia-Qi Wang, Qiuli Zhang
Background: MiR-106b and caspase-8 played a key role in the development of acute glaucoma. Increasing evidence has indicated that long non-coding RNA (lncRNA) maternally expressed gene 3 (MEG3) participated in regulating pathophysiological processes. However, the association among MEG3, miR-106b and caspase-8 remained unclear. Methods: We employed the mouse model of acute glaucoma and oxygen and glucose deprivation (OGD)/reoxygenation cellular model for in vivo and in vitro experiments. The miRNA inhibitor and small interfering RNA (siRNA) were transfected into primary retinal ganglion cells (RGCs) for miRNA and lncRNA knockdown. The interaction among MEG3, miR-106b and caspase-8 was assessed by RNA immunoprecipitation, RNA pull down and luciferase reporter assay. The changes in gene expression were assessed by quantitative Real-Time PCR (qRT-PCR) and western blot. Cell apoptosis analysis was performed using flow cytometry. Results: MEG3 expression was increased in the mouse model of acute glaucoma and OGD-treated RGCs. MEG3 knockdown alleviated RGC apoptosis following OGD. RNA immunoprecipitation and RNA pull down displayed that MEG3 directly targeted miR-106b, while luciferase reporter assay confirmed the interaction between miR-106b and caspase-8. MEG3 silencing significantly relieved RGC apoptosis via downregulating miR-106b target gene caspase-8. Conclusion: MEG3 increased the apoptosis of glaucomatous RGC via miR-106b/caspase-8 axis. Keywords: acute glaucoma; retinal ganglion cells; MEG3; miR-106b; caspase-8
{"title":"LncRNA MEG3 promotes glaucomatous retinal ganglion cell apoptosis via upregulating miR-106 target gene caspase-8","authors":"Kaidi Sun, Jia-Qi Wang, Qiuli Zhang","doi":"10.31491/csrc.2019.09.002","DOIUrl":"https://doi.org/10.31491/csrc.2019.09.002","url":null,"abstract":"Background: MiR-106b and caspase-8 played a key role in the development of acute glaucoma. Increasing evidence has indicated that long non-coding RNA (lncRNA) maternally expressed gene 3 (MEG3) participated in regulating pathophysiological processes. However, the association among MEG3, miR-106b and caspase-8 remained unclear. Methods: We employed the mouse model of acute glaucoma and oxygen and glucose deprivation (OGD)/reoxygenation cellular model for in vivo and in vitro experiments. The miRNA inhibitor and small interfering RNA (siRNA) were transfected into primary retinal ganglion cells (RGCs) for miRNA and lncRNA knockdown. The interaction among MEG3, miR-106b and caspase-8 was assessed by RNA immunoprecipitation, RNA pull down and luciferase reporter assay. The changes in gene expression were assessed by quantitative Real-Time PCR (qRT-PCR) and western blot. Cell apoptosis analysis was performed using flow cytometry. Results: MEG3 expression was increased in the mouse model of acute glaucoma and OGD-treated RGCs. MEG3 knockdown alleviated RGC apoptosis following OGD. RNA immunoprecipitation and RNA pull down displayed that MEG3 directly targeted miR-106b, while luciferase reporter assay confirmed the interaction between miR-106b and caspase-8. MEG3 silencing significantly relieved RGC apoptosis via downregulating miR-106b target gene caspase-8. Conclusion: MEG3 increased the apoptosis of glaucomatous RGC via miR-106b/caspase-8 axis. Keywords: acute glaucoma; retinal ganglion cells; MEG3; miR-106b; caspase-8","PeriodicalId":158678,"journal":{"name":"Clinical Surgery Research Communications","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130232104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-09-29DOI: 10.31491/csrc.2019.09.005
W. A. Arab, D. Alshehab, Hassan Jamal Eddine, A. Ayed
Respiratory papillomas are rare benign lesions. They are characterized by the appearance of papillomatous lesions anywhere in the tracheobronchial tree. It affects children and young adults. Although the disease affects mainly the upper airways, it may be aggressive and extends distally to the lower respiratory tract and pulmonary parenchyma. The course of the disease is unpredictable. It may regress spontaneously, but in other instances, it may lead to serious complications ranging from airway obstruction up to malignant transformation. Surgical excision is the mainstay of definitive treatment to prevent recurrence and to exclude malignancy. We have reviewed the literature for this rare type of respiratory disease and present a thirty-five years old; male patient who had chronic cough and was referred to our unit with a CT-chest that revealed left main endo-bronchial lesion. Bronchoscopy and biopsy of the lesion were performed and revealed squamous papilloma. Final treatment introduced to the patient was endoscopic thermal ablation. However, the final pathology revealed complicated squamous bronchial papilloma with infection by aspergillosis and actinomycosis. Key words: HPV; Tracheo-bronchial papilmomatosis; endo-broncial lesion; bronchoscopy
{"title":"Complicated tracheo-bronchial papillomatosis","authors":"W. A. Arab, D. Alshehab, Hassan Jamal Eddine, A. Ayed","doi":"10.31491/csrc.2019.09.005","DOIUrl":"https://doi.org/10.31491/csrc.2019.09.005","url":null,"abstract":"Respiratory papillomas are rare benign lesions. They are characterized by the appearance of papillomatous lesions anywhere in the tracheobronchial tree. It affects children and young adults. Although the disease affects mainly the upper airways, it may be aggressive and extends distally to the lower respiratory tract and pulmonary parenchyma. The course of the disease is unpredictable. It may regress spontaneously, but in other instances, it may lead to serious complications ranging from airway obstruction up to malignant transformation. Surgical excision is the mainstay of definitive treatment to prevent recurrence and to exclude malignancy. We have reviewed the literature for this rare type of respiratory disease and present a thirty-five years old; male patient who had chronic cough and was referred to our unit with a CT-chest that revealed left main endo-bronchial lesion. Bronchoscopy and biopsy of the lesion were performed and revealed squamous papilloma. Final treatment introduced to the patient was endoscopic thermal ablation. However, the final pathology revealed complicated squamous bronchial papilloma with infection by aspergillosis and actinomycosis. Key words: HPV; Tracheo-bronchial papilmomatosis; endo-broncial lesion; bronchoscopy","PeriodicalId":158678,"journal":{"name":"Clinical Surgery Research Communications","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134445887","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-09-29DOI: 10.31491/csrc.2019.09.001
M. W. Dougaz, Mohamed Ali Chaouch, Houcine Magherbi, M. Khalfallah, H. Jerraya, I. Bouasker, R. Nouira, C. Dziri
Background: The most frequent complication of liver hydatid cyst is intrabiliary rupture (LHCIBR). This study aimed to investigate preoperative predictive factors of occult and frank LHCIBR. Methods: We conducted a retrospective study concerning patients operated on consecutively for liver hydatidosis for 2 years. Patients were divided into three groups: who had no intrabiliary rupture, patients who had an occult rupture and patients who had a frank rupture. Results: We recorded 56 patients with 82 liver hydatid cysts. LHCIBR was occult in 16 cysts and frank in four cysts. Bivariate analysis identified jaundice and cyst size as associated with frank LHCIBR and US cyst type II or III, recurrent cyst, and size of the hydatid cyst as associated with occult LHCIBR. In the multivariate analysis, we retained jaundice, cyst size > 6.5 cm and duration of symptoms > 45 days as significant predictive factors of frank rupture and cyst size > 6.5 cm, number of recurrences ≥ 3, cyst type II or III, leukocytosis > 9.000/mm3 and eosinophilia > 5.5% as significant of occult rupture. Conclusion: Misdiagnosis LHCIBR can lead to increased morbidity and mortality. They were avoided by predicting cyst rupture, correct timing and type surgery, proper drainage and preoperative intensive care of patients. Keywords: Liver hydatid cyst; Occult intra-biliary rupture; Frank intra-biliary rupture; complication; predictive factors.
{"title":"Preoperative predictive factors of liver hydatid cyst occult or frank intrabiliary rupture","authors":"M. W. Dougaz, Mohamed Ali Chaouch, Houcine Magherbi, M. Khalfallah, H. Jerraya, I. Bouasker, R. Nouira, C. Dziri","doi":"10.31491/csrc.2019.09.001","DOIUrl":"https://doi.org/10.31491/csrc.2019.09.001","url":null,"abstract":"Background: The most frequent complication of liver hydatid cyst is intrabiliary rupture (LHCIBR). This study aimed to investigate preoperative predictive factors of occult and frank LHCIBR. Methods: We conducted a retrospective study concerning patients operated on consecutively for liver hydatidosis for 2 years. Patients were divided into three groups: who had no intrabiliary rupture, patients who had an occult rupture and patients who had a frank rupture. Results: We recorded 56 patients with 82 liver hydatid cysts. LHCIBR was occult in 16 cysts and frank in four cysts. Bivariate analysis identified jaundice and cyst size as associated with frank LHCIBR and US cyst type II or III, recurrent cyst, and size of the hydatid cyst as associated with occult LHCIBR. In the multivariate analysis, we retained jaundice, cyst size > 6.5 cm and duration of symptoms > 45 days as significant predictive factors of frank rupture and cyst size > 6.5 cm, number of recurrences ≥ 3, cyst type II or III, leukocytosis > 9.000/mm3 and eosinophilia > 5.5% as significant of occult rupture. Conclusion: Misdiagnosis LHCIBR can lead to increased morbidity and mortality. They were avoided by predicting cyst rupture, correct timing and type surgery, proper drainage and preoperative intensive care of patients. Keywords: Liver hydatid cyst; Occult intra-biliary rupture; Frank intra-biliary rupture; complication; predictive factors.","PeriodicalId":158678,"journal":{"name":"Clinical Surgery Research Communications","volume":"313 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116119490","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-06-25DOI: 10.31491/csrc.2019.06.032
Tengfei Wu, Y. Liu, Xiuquan Guo
Objective: The aim of this study was to reveal the role of CASC15 and miR-139-5p function in regulating agerelated cataracts (ARC). Methods: The expression of CASC15, miR-139-5p and caspase-3 were detected by qRT-PCR. Western blot was used to analyze the level of apoptosis-related proteins. Flow cytometry was used to measure the apoptosis of SAR01/04l cells treated with UV irradiation. Results: The expression of CASC15 and caspase-3 in the lens of ARC was higher than that in normal lens, but the expression of miR-139-5p had opposite trend. The level of caspase-3 and Bax were increased and Bcl-2 was decreased in ARC group. In addition, CASC15 negatively regulated miR-139-5p expression and promoted SAR01/04l cell apoptosis. CASC15 could combine with miR-139-5p, and miR-139-5p could combine with caspase-3 3’-UTR. Furthermore, pcDNA-caspase-3 could reverse the inhibitory effect of si-CASC15 and miR139-5p mimic on SRA01/04 cell apoptosis. Conclusion: CASC15 promoted lens epithelial cell apoptosis by regulating miR-139-5p/caspase-3. Keywords: ARC; CASC15; LECs apoptosis; miR-139-5p
目的:本研究的目的是揭示cas15和miR-139-5p功能在调节年龄相关性白内障(ARC)中的作用。方法:采用qRT-PCR检测cas15、miR-139-5p、caspase-3的表达。Western blot检测细胞凋亡相关蛋白水平。流式细胞术检测紫外线照射后SAR01/04l细胞的凋亡情况。结果:ARC晶状体中cas15和caspase-3的表达高于正常晶状体，而miR-139-5p的表达则相反。ARC组caspase-3、Bax水平升高，Bcl-2水平降低。此外，CASC15负调控miR-139-5p表达，促进SAR01/04l细胞凋亡。cas15可与miR-139-5p结合，miR-139-5p可与caspase-3 3′-UTR结合。此外，pcDNA-caspase-3可以逆转si-CASC15和miR139-5p mimic对SRA01/04细胞凋亡的抑制作用。结论:cas15通过调控miR-139-5p/caspase-3促进晶状体上皮细胞凋亡。关键词:弧;CASC15;lec细胞凋亡;mir - 139 - 5 - p
{"title":"CASC15 Promotes Lens Epithelial Cell Apoptosis in Age-Related Cataracts by Regulating MiR-139-5p/caspase-3","authors":"Tengfei Wu, Y. Liu, Xiuquan Guo","doi":"10.31491/csrc.2019.06.032","DOIUrl":"https://doi.org/10.31491/csrc.2019.06.032","url":null,"abstract":"Objective: The aim of this study was to reveal the role of CASC15 and miR-139-5p function in regulating agerelated cataracts (ARC). Methods: The expression of CASC15, miR-139-5p and caspase-3 were detected by qRT-PCR. Western blot was used to analyze the level of apoptosis-related proteins. Flow cytometry was used to measure the apoptosis of SAR01/04l cells treated with UV irradiation. Results: The expression of CASC15 and caspase-3 in the lens of ARC was higher than that in normal lens, but the expression of miR-139-5p had opposite trend. The level of caspase-3 and Bax were increased and Bcl-2 was decreased in ARC group. In addition, CASC15 negatively regulated miR-139-5p expression and promoted SAR01/04l cell apoptosis. CASC15 could combine with miR-139-5p, and miR-139-5p could combine with caspase-3 3’-UTR. Furthermore, pcDNA-caspase-3 could reverse the inhibitory effect of si-CASC15 and miR139-5p mimic on SRA01/04 cell apoptosis. Conclusion: CASC15 promoted lens epithelial cell apoptosis by regulating miR-139-5p/caspase-3. Keywords: ARC; CASC15; LECs apoptosis; miR-139-5p","PeriodicalId":158678,"journal":{"name":"Clinical Surgery Research Communications","volume":"14 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124020946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-06-25DOI: 10.31491/csrc.2019.06.031
Xiao-Bin Gu
Background: Programmed cell death-ligand 1 (PD-L1) was reported to be associated with survival outcomes in patients with osteosarcoma, but the results were controversial. This study aimed to evaluate the prognostic value of PD-L1 in osteosarcoma. Methods: The pooled HR and 95%CI were calculated to measure the prognostic role of PD-L1 for overall survival (OS) and disease-free survival (DFS). The odds ratio (OR) and 95%CI were used to evaluate the correlation of PD-L1 and clinicopathological features. Publication bias was measured using Begg’s funnel plots. Results: A total of 9 studies with 538 patients were included in this meta-analysis. The pooled results were HR=1.78, 95%CI=1.36-2.32, p<0.001 for OS and HR=1.24, 95%CI=0.31-5.07, p=0.761 for DFS. PD-L1 was significantly associated with metastasis (OR=8.51, 95%CI=4.3-16.86, p<0.001). Conclusion: PD-L1 might be a potential prognostic marker in patients with osteosarcoma. Keywords: meta-analysis; PD-L1; osteosarcoma; prognosis
{"title":"Prognostic and Clinicopathological Significance of Programmed Death-Ligand 1 in Osteosarcoma: a Meta-Analysis","authors":"Xiao-Bin Gu","doi":"10.31491/csrc.2019.06.031","DOIUrl":"https://doi.org/10.31491/csrc.2019.06.031","url":null,"abstract":"Background: Programmed cell death-ligand 1 (PD-L1) was reported to be associated with survival outcomes in patients with osteosarcoma, but the results were controversial. This study aimed to evaluate the prognostic value of PD-L1 in osteosarcoma. Methods: The pooled HR and 95%CI were calculated to measure the prognostic role of PD-L1 for overall survival (OS) and disease-free survival (DFS). The odds ratio (OR) and 95%CI were used to evaluate the correlation of PD-L1 and clinicopathological features. Publication bias was measured using Begg’s funnel plots. Results: A total of 9 studies with 538 patients were included in this meta-analysis. The pooled results were HR=1.78, 95%CI=1.36-2.32, p<0.001 for OS and HR=1.24, 95%CI=0.31-5.07, p=0.761 for DFS. PD-L1 was significantly associated with metastasis (OR=8.51, 95%CI=4.3-16.86, p<0.001). Conclusion: PD-L1 might be a potential prognostic marker in patients with osteosarcoma. Keywords: meta-analysis; PD-L1; osteosarcoma; prognosis","PeriodicalId":158678,"journal":{"name":"Clinical Surgery Research Communications","volume":"75 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126180565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-06-25DOI: 10.31491/csrc.2019.06.030
Heng-jun Zhou, Xiu-jue Zheng, F. Jian, Yue-hui Ma, R. Zhan
Background: Thoracic intervertebral foramen (TIF) lesions were really difficult to expose through posterior or transforaminal approaches. There was no effective treatment strategy, especially for complex ones. Methods: Between 2013 and 2015, 22 patients presenting with complex TIF lesions underwent posterior approach combined with TIF posterior wall opening and spinal reconstruction were retrospectively reviewed. After ascertaining the precise location of complex TIF lesions, incisions should allow for exposure of the lesion’s adjacent vertebral pedicle screw entry point. The adjacent pair of vertebral lamina and spinous processes is typically removed in a single piece, such that the intra-spinal section can be fully exposed. The posterior wall of the TIF is subsequently opened, allowing the intra-TIF and extra-spinal sections to be sufficiently exposed. Subsequently, spinal reconstruction at the appropriate level is required. When the ventral spinal column is intact, dorsal reconstruction is typically performed using the screw and rod fixation technique (SRFT). After the dorsal surface of the spine has been burnished, the removed vertebral lamina and spinous process are rendered granular to allow reconstruction of the TIF and fusion of adjacent vertebrae. Results: All patients presenting with complex TIF lesions were successfully treated with this approach. No patient had experienced any complications arising from SRFT use. No patient suffered intraspinal infection, incision splits, or CSF leakage in the short-term (between 1 and 3 months) or fixation loosening or spinal instability in the long-term (6–24 months). Imaging studies confirmed that the TIF and spinal reconstruction outcomes were favorable. Conclusion: A posterior approach combined with TIF posterior wall opening and spinal reconstruction is a rapid, effective, and reliable method in the treatment of complex TIF lesions. This technique can provide total lesion resection while reconstructing spinal stability. Keywords: posterior approach; thoracic intervertebral foramen (TIF); intervertebral articular process (IAP); spinal reconstruction; screw and rod fixation technique (SRFT)
{"title":"Posterior Approach Combined with Thoracic Intervertebral Foramen Posterior Wall Opening and Spinal Reconstruction for Complex Thoracic Intervertebral Foramen Lesions: a Single-Centre Experience","authors":"Heng-jun Zhou, Xiu-jue Zheng, F. Jian, Yue-hui Ma, R. Zhan","doi":"10.31491/csrc.2019.06.030","DOIUrl":"https://doi.org/10.31491/csrc.2019.06.030","url":null,"abstract":"Background: Thoracic intervertebral foramen (TIF) lesions were really difficult to expose through posterior or transforaminal approaches. There was no effective treatment strategy, especially for complex ones. Methods: Between 2013 and 2015, 22 patients presenting with complex TIF lesions underwent posterior approach combined with TIF posterior wall opening and spinal reconstruction were retrospectively reviewed. After ascertaining the precise location of complex TIF lesions, incisions should allow for exposure of the lesion’s adjacent vertebral pedicle screw entry point. The adjacent pair of vertebral lamina and spinous processes is typically removed in a single piece, such that the intra-spinal section can be fully exposed. The posterior wall of the TIF is subsequently opened, allowing the intra-TIF and extra-spinal sections to be sufficiently exposed. Subsequently, spinal reconstruction at the appropriate level is required. When the ventral spinal column is intact, dorsal reconstruction is typically performed using the screw and rod fixation technique (SRFT). After the dorsal surface of the spine has been burnished, the removed vertebral lamina and spinous process are rendered granular to allow reconstruction of the TIF and fusion of adjacent vertebrae. Results: All patients presenting with complex TIF lesions were successfully treated with this approach. No patient had experienced any complications arising from SRFT use. No patient suffered intraspinal infection, incision splits, or CSF leakage in the short-term (between 1 and 3 months) or fixation loosening or spinal instability in the long-term (6–24 months). Imaging studies confirmed that the TIF and spinal reconstruction outcomes were favorable. Conclusion: A posterior approach combined with TIF posterior wall opening and spinal reconstruction is a rapid, effective, and reliable method in the treatment of complex TIF lesions. This technique can provide total lesion resection while reconstructing spinal stability. Keywords: posterior approach; thoracic intervertebral foramen (TIF); intervertebral articular process (IAP); spinal reconstruction; screw and rod fixation technique (SRFT)","PeriodicalId":158678,"journal":{"name":"Clinical Surgery Research Communications","volume":"31 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127512464","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-03-25DOI: 10.31491/CSRC.2019.03.027
Eerdunduleng Eerdunduleng, Yang Liu, Yan‐fu Wang
Background: Pancreatic cancer (PC) is one of the most lethal types of cancer in the world. The complex network of non-coding RNAs has been demonstrate to involved in the PC progression, however, the potential mechanism was still unclear. Methods: The clinical tumor tissues and the adjacent-tumor tissues of PC were obtained from the surgery. Real-time PCR and western blot were performed to detect gene expression in appropriate manner. The interaction between circ-LDLRAD3 and miR-876-3p was determined using luciferase reporter assay and RNA pull-down. The interaction between miR-876-3p and STAT3 was determined using luciferase reporter assay. Results: Overexpressed circ-LDLRAD3 and STAT3, while down-regulated miR-876-3p was observed in both PC tissues and cell lines. Knockdown of circ-LDLRAD3 suppressed PC cell proliferation, migration and invasion. circ-LDLRAD3 directly regulated the expression of miR-876-3p. STAT3 is the target molecular of mR-876-3p. circ-LDLRAD3 regulated the expression of STAT3 by miR-876-3p. circ-LDLRAD3 regulated cell proliferation, migration and invasion by miR-876-3p. Conclusion: Down-regulated circ-LDLRAD3 suppressed cell proliferation, invasion and migration by directly regulating miR-876-3p/STAT3. Key words: pancreatic cancer; circ-LDLRAD3; miR-876-3p; STAT3; cell proliferation; migration and invasion
{"title":"circ-LDLRAD3 regulates cell proliferation, migration and invasion of pancreatic cancer by miR-876-3p/STAT3","authors":"Eerdunduleng Eerdunduleng, Yang Liu, Yan‐fu Wang","doi":"10.31491/CSRC.2019.03.027","DOIUrl":"https://doi.org/10.31491/CSRC.2019.03.027","url":null,"abstract":"Background: Pancreatic cancer (PC) is one of the most lethal types of cancer in the world. The complex network of non-coding RNAs has been demonstrate to involved in the PC progression, however, the potential mechanism was still unclear. Methods: The clinical tumor tissues and the adjacent-tumor tissues of PC were obtained from the surgery. Real-time PCR and western blot were performed to detect gene expression in appropriate manner. The interaction between circ-LDLRAD3 and miR-876-3p was determined using luciferase reporter assay and RNA pull-down. The interaction between miR-876-3p and STAT3 was determined using luciferase reporter assay. Results: Overexpressed circ-LDLRAD3 and STAT3, while down-regulated miR-876-3p was observed in both PC tissues and cell lines. Knockdown of circ-LDLRAD3 suppressed PC cell proliferation, migration and invasion. circ-LDLRAD3 directly regulated the expression of miR-876-3p. STAT3 is the target molecular of mR-876-3p. circ-LDLRAD3 regulated the expression of STAT3 by miR-876-3p. circ-LDLRAD3 regulated cell proliferation, migration and invasion by miR-876-3p. Conclusion: Down-regulated circ-LDLRAD3 suppressed cell proliferation, invasion and migration by directly regulating miR-876-3p/STAT3. Key words: pancreatic cancer; circ-LDLRAD3; miR-876-3p; STAT3; cell proliferation; migration and invasion","PeriodicalId":158678,"journal":{"name":"Clinical Surgery Research Communications","volume":"205 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132151772","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-20DOI: 10.31491/CSRC.2019.03.029
Ying Ding, Wei-liang Jiang, Bin Wen, Yang He, T. Zhu
Background : Peripartum cardiomyopathy (PPCM) is rare heart disease that threatens the lives of pregnant women. Patient : In the current report, we reported a case of a 40-week pregnant patient with PPCM. Diagnoses : The patient was diagnosed with PPCM. Intervention : The patient underwent emergency cesarean section and received other supportive treatments, mainly including cardiotonic, diuretic, bromocriptine, hemodialysis, improvement of cardiac function, blood-pressure-lowering, hepatic protection, supplementing albumin, delactation, promoting uterine contraction, the noninvasive ventilator-assisted ventilation improved oxygen delivery and CRRT reduced cardiac load. Outcomes : The relative clinical symptoms have significantly become better after 58 days treatment and the patient has discharged. Lessons : This case emphasized the significance of early diagnosis of PPCM in pregnant women.
{"title":"A case of perinatal cardiomyopathy","authors":"Ying Ding, Wei-liang Jiang, Bin Wen, Yang He, T. Zhu","doi":"10.31491/CSRC.2019.03.029","DOIUrl":"https://doi.org/10.31491/CSRC.2019.03.029","url":null,"abstract":"Background : Peripartum cardiomyopathy (PPCM) is rare heart disease that threatens the lives of pregnant women. Patient : In the current report, we reported a case of a 40-week pregnant patient with PPCM. Diagnoses : The patient was diagnosed with PPCM. Intervention : The patient underwent emergency cesarean section and received other supportive treatments, mainly including cardiotonic, diuretic, bromocriptine, hemodialysis, improvement of cardiac function, blood-pressure-lowering, hepatic protection, supplementing albumin, delactation, promoting uterine contraction, the noninvasive ventilator-assisted ventilation improved oxygen delivery and CRRT reduced cardiac load. Outcomes : The relative clinical symptoms have significantly become better after 58 days treatment and the patient has discharged. Lessons : This case emphasized the significance of early diagnosis of PPCM in pregnant women.","PeriodicalId":158678,"journal":{"name":"Clinical Surgery Research Communications","volume":"174 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114435240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This study reported a case of late-staged esophageal carcinoma, complicated by severe dyspha late onset tracheal stenosis after treating with 125 Iodine seed stent implantation. The patient had progressive stenosis of the lower segment (left main trachea) at 6, 26, and 47 days postoperatively. The cause of stenosis might be related to direct press by stent, tumor proliferation, the aortic arch with violate pulsate and the complications caused by other therapies. Due to its short clinical utilization, 125 Iodine seed stent may present some fatal complications. Therefore, the long-term efficacy of 125 Iodine seed stent remains to be studied. Keyword: 125 Iodine seed, esophagus stent, late onset, tracheal stenosis, complication
{"title":"Interventional therapy of late onset tracheal stenosis after implantation of 125Iodine seed esophageal stent","authors":"Yong-Zhen Wang, Yan-Hua Wang, Shao-Tong Lai, Feng Dai, Hai-Feng Xu, Hui Chen, Ren-Jie Yang","doi":"10.31491/CSRC.2019.03.028","DOIUrl":"https://doi.org/10.31491/CSRC.2019.03.028","url":null,"abstract":"This study reported a case of late-staged esophageal carcinoma, complicated by severe dyspha late onset tracheal stenosis after treating with 125 Iodine seed stent implantation. The patient had progressive stenosis of the lower segment (left main trachea) at 6, 26, and 47 days postoperatively. The cause of stenosis might be related to direct press by stent, tumor proliferation, the aortic arch with violate pulsate and the complications caused by other therapies. Due to its short clinical utilization, 125 Iodine seed stent may present some fatal complications. Therefore, the long-term efficacy of 125 Iodine seed stent remains to be studied. Keyword: 125 Iodine seed, esophagus stent, late onset, tracheal stenosis, complication","PeriodicalId":158678,"journal":{"name":"Clinical Surgery Research Communications","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129398205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-20DOI: 10.31491/CSRC.2019.03.026
Wanru Geng, Yu Sun, Yan Jiang, K. Sun
Objective : It has been reported that high tumor-associated macrophages (TAMs) numbers in tumors were correlated with poor tumor responses to irradiation. However, few studies have attempted to demonstrate the roles of TAMs in radiotherapy for suppressing the progression of lung cancer. Methods : TAMs were separated from C57BL/6 mice which were inoculated Lewis lung cancer cells and exposed to radiation therapy, then the expression of maker genes of polarized macrophages and lincRNA-p21 were analyzed using qPCR. Cell viability and invasion of Lewis cells cultured with TAMs exposed to radiation therapy were detected with CKK-8 assay and cell invasion assay, respectively. Ad-LincRNA-p21 and Ad-Si-LincRNA-p21 were used to examine the effect of lincRNA-p21 on TAMs. Results : Radiotherapy effectively suppressed the tumor growth in C57BL/6 mice transplanted with Lewis lung cancer cell. Radiotherapy promoted the polarized of TEMs into M1 macrophage and up-regulated the level of lincRNA-p21 in TEMs. Moreover, TEMs transfected with Ad-LincRNA-p21 presented anti-tumor effect and Ad-Si-LincRNA-p21 might reverse the effect of radiotherapy on tumor growth. Conclusion : Our results suggest that radiotherapy promotes the polarization of TEMs into M1 macrophage to suppress lung cancer progression and lincRNA-p21 plays an important role in the radiotherapy treatment for lung cancer. Keywords: radiotherapy; polarization; tumor-associated macrophage; lung cancer; lincRNA-p21
{"title":"Radiotherapy alters the polarization of tumor-associated macrophage to suppress lung cancer progression via up-regulation of lincRNA-p21","authors":"Wanru Geng, Yu Sun, Yan Jiang, K. Sun","doi":"10.31491/CSRC.2019.03.026","DOIUrl":"https://doi.org/10.31491/CSRC.2019.03.026","url":null,"abstract":"Objective : It has been reported that high tumor-associated macrophages (TAMs) numbers in tumors were correlated with poor tumor responses to irradiation. However, few studies have attempted to demonstrate the roles of TAMs in radiotherapy for suppressing the progression of lung cancer. Methods : TAMs were separated from C57BL/6 mice which were inoculated Lewis lung cancer cells and exposed to radiation therapy, then the expression of maker genes of polarized macrophages and lincRNA-p21 were analyzed using qPCR. Cell viability and invasion of Lewis cells cultured with TAMs exposed to radiation therapy were detected with CKK-8 assay and cell invasion assay, respectively. Ad-LincRNA-p21 and Ad-Si-LincRNA-p21 were used to examine the effect of lincRNA-p21 on TAMs. Results : Radiotherapy effectively suppressed the tumor growth in C57BL/6 mice transplanted with Lewis lung cancer cell. Radiotherapy promoted the polarized of TEMs into M1 macrophage and up-regulated the level of lincRNA-p21 in TEMs. Moreover, TEMs transfected with Ad-LincRNA-p21 presented anti-tumor effect and Ad-Si-LincRNA-p21 might reverse the effect of radiotherapy on tumor growth. Conclusion : Our results suggest that radiotherapy promotes the polarization of TEMs into M1 macrophage to suppress lung cancer progression and lincRNA-p21 plays an important role in the radiotherapy treatment for lung cancer. Keywords: radiotherapy; polarization; tumor-associated macrophage; lung cancer; lincRNA-p21","PeriodicalId":158678,"journal":{"name":"Clinical Surgery Research Communications","volume":"25 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126988640","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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