Pub Date : 2018-12-25DOI: 10.31491/CSRC.2018.12.023
Yongxiong He, L. Bin, Bokang Lv, Xing Wang, A. Erbin, F. Gao, Yanqiang Huan, Leonora Chao, Jian-feng Liu, Fei Wang
Background: Posterior cervical atlantoaxial pedicle screw fixation is a very effective treatment for atlantoaxial instability (AAI). However, due to the complex anatomy of the cranial-cervical junction, the accuracy and safety of posterior atlantoaxial pedicle screw placement remains extremely challenging. Objective: To quantitatively evaluate the safety and accuracy of the 3D navigation module to assist the posterior atlantoaxial fixation. Methods: A total of 20 AAI patients were selected between June 2014 and September 2015. The Mimics v10.1 and 3-matic software were used. The 3D navigation module was designed as a double-sided positioning hole guide with a guide rod. All patients underwent posterior atlantoaxial posterior pedicle screw fixation with 3D navigation module. The actual entry point and screw trajectory were measured after operation, which were compared with the ideal entry point and screw trajectory. The Japanese Orthopaedic Association (JOA) score was measured before and after surgery to evaluate the neurological function improvement. The average operation time, blood loss, and frequency of intraoperative fluoroscopy were counted. Results: The posterior atlantoaxial pedicle screw fixation with a 3D navigation module was successfully performed in all patients. A total of 80 atlantoaxial pedicle screws were implanted in the 20 patients. Postoperative CT scan showed that two pedicle screws deviated from the medial aspect of the atlas pedicle cortex and entered the spinal canal approximately 1 mm, without causing neurological complications. There was no significant difference between the ideal and actual entry points or ideal and actual screw trajectories of the atlas and axis (P > 0.05). The preoperative JOA score was 12.45 ± 1.15 and postoperative JOA score was 15.5 ± 0.89, with statistically significant difference (P < 0.05). Conclusion: It was safe and effective to use the 3D navigation module to assist the posterior atlantoaxial pedicle insertion, with a high accuracy of pedicle screw placement. Keywords: atlantoaxial instability, pedicle screw, 3D navigation module, rapid prototyping
{"title":"Clinical Application and Efficacy Analysis of 3D Navigation Module in the Treatment of Atlantoaxial Instability","authors":"Yongxiong He, L. Bin, Bokang Lv, Xing Wang, A. Erbin, F. Gao, Yanqiang Huan, Leonora Chao, Jian-feng Liu, Fei Wang","doi":"10.31491/CSRC.2018.12.023","DOIUrl":"https://doi.org/10.31491/CSRC.2018.12.023","url":null,"abstract":"Background: Posterior cervical atlantoaxial pedicle screw fixation is a very effective treatment for atlantoaxial instability (AAI). However, due to the complex anatomy of the cranial-cervical junction, the accuracy and safety of posterior atlantoaxial pedicle screw placement remains extremely challenging. Objective: To quantitatively evaluate the safety and accuracy of the 3D navigation module to assist the posterior atlantoaxial fixation. Methods: A total of 20 AAI patients were selected between June 2014 and September 2015. The Mimics v10.1 and 3-matic software were used. The 3D navigation module was designed as a double-sided positioning hole guide with a guide rod. All patients underwent posterior atlantoaxial posterior pedicle screw fixation with 3D navigation module. The actual entry point and screw trajectory were measured after operation, which were compared with the ideal entry point and screw trajectory. The Japanese Orthopaedic Association (JOA) score was measured before and after surgery to evaluate the neurological function improvement. The average operation time, blood loss, and frequency of intraoperative fluoroscopy were counted. Results: The posterior atlantoaxial pedicle screw fixation with a 3D navigation module was successfully performed in all patients. A total of 80 atlantoaxial pedicle screws were implanted in the 20 patients. Postoperative CT scan showed that two pedicle screws deviated from the medial aspect of the atlas pedicle cortex and entered the spinal canal approximately 1 mm, without causing neurological complications. There was no significant difference between the ideal and actual entry points or ideal and actual screw trajectories of the atlas and axis (P > 0.05). The preoperative JOA score was 12.45 ± 1.15 and postoperative JOA score was 15.5 ± 0.89, with statistically significant difference (P < 0.05). Conclusion: It was safe and effective to use the 3D navigation module to assist the posterior atlantoaxial pedicle insertion, with a high accuracy of pedicle screw placement. Keywords: atlantoaxial instability, pedicle screw, 3D navigation module, rapid prototyping","PeriodicalId":158678,"journal":{"name":"Clinical Surgery Research Communications","volume":"32 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116939658","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-09-25DOI: 10.31491/CSRC.2018.9.019
Bangjun Cheng, Yi Luo, Jun-wu Huang, Xiao-Bing Feng
Objective : To compare and analyze the clinical efficacy of spiral fracture of the lower third of the tibia shaft combined with a posterior malleolar fracture treated with a plate or hollow screws. Methods : Surgical treatment was carried out in 61 patients with a spiral fracture of the lower third of the tibia shaft combined with a posterior malleolar fracture. After the tibia fractures were fixed with a plate, the posterior malleolus fractures were repaired using either three hollow screws (hollow screw group, 34 cases) or a plate (plate group, 27 cases). The ankle and foot scoring system recommended by Baird-Jackson was used to evaluate the ankle postoperatively. Results: All 61 patients were followed-up; the follow-up period ranged from 6–36 months, with an average of 20.8 months. All fractures healed well, and no delayed unions or nonunions occurred. The function of the ankle joint according to the Baird-Jackson score system evaluation was as follows: in the hollow screw group, 18 were excellent, 10 were good, 5 were fair, and 1 was poor, with at least 82.4% experiencing good or excellent outcomes. In the plate group, 8 were excellent, 9 were good, 3 were fair, and 1 was poor, with 85.2% reporting excellent or good results. The difference in these two groups of Baird-Jackson scores with an excellent rate was not statistically significant (P =0.21>0.05). Conclusion : Spiral fractures of the distal third of the tibia are often accompanied by posterior malleolar fractures. Clinical treatment of the posterior malleolar fracture had no obvious impact on the clinical curative effect of hollow screw and L-type plates. Slight trauma was observed with hollow screw internal fixation, while internal plate fixation was more reliable. Keywords ： Spiral tibia fracture; Posterior malleolar fracture; Hollow screw; Plate; Internal Fixation
{"title":"A comparative study of the clinical results of two treatment methods for spiral fracture of the lower third of the tibia shaft combined with posterior malleolar fracture","authors":"Bangjun Cheng, Yi Luo, Jun-wu Huang, Xiao-Bing Feng","doi":"10.31491/CSRC.2018.9.019","DOIUrl":"https://doi.org/10.31491/CSRC.2018.9.019","url":null,"abstract":"Objective : To compare and analyze the clinical efficacy of spiral fracture of the lower third of the tibia shaft combined with a posterior malleolar fracture treated with a plate or hollow screws. Methods : Surgical treatment was carried out in 61 patients with a spiral fracture of the lower third of the tibia shaft combined with a posterior malleolar fracture. After the tibia fractures were fixed with a plate, the posterior malleolus fractures were repaired using either three hollow screws (hollow screw group, 34 cases) or a plate (plate group, 27 cases). The ankle and foot scoring system recommended by Baird-Jackson was used to evaluate the ankle postoperatively. Results: All 61 patients were followed-up; the follow-up period ranged from 6–36 months, with an average of 20.8 months. All fractures healed well, and no delayed unions or nonunions occurred. The function of the ankle joint according to the Baird-Jackson score system evaluation was as follows: in the hollow screw group, 18 were excellent, 10 were good, 5 were fair, and 1 was poor, with at least 82.4% experiencing good or excellent outcomes. In the plate group, 8 were excellent, 9 were good, 3 were fair, and 1 was poor, with 85.2% reporting excellent or good results. The difference in these two groups of Baird-Jackson scores with an excellent rate was not statistically significant (P =0.21>0.05). Conclusion : Spiral fractures of the distal third of the tibia are often accompanied by posterior malleolar fractures. Clinical treatment of the posterior malleolar fracture had no obvious impact on the clinical curative effect of hollow screw and L-type plates. Slight trauma was observed with hollow screw internal fixation, while internal plate fixation was more reliable. Keywords ： Spiral tibia fracture; Posterior malleolar fracture; Hollow screw; Plate; Internal Fixation","PeriodicalId":158678,"journal":{"name":"Clinical Surgery Research Communications","volume":"31 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133806699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A mature ovarian teratoma is a common benign germ cell tumor of the ovary in women that rarely causes a malignant transformation. However, a malignant transformation can occur in the ectodermal, mesodermal, and endodermal tissues of the tumor. A squamous cell carcinoma is the most common and an adenocarcinoma is the second most common malignant tumor derived from a cystic teratoma. Due to the rarity of malignant transformations in mature teratomas and the lack of clinical data, the clinical pathological features, malignant transformation mechanism, therapeutic methods, and prognosis-relevant factors are unclear. Currently, there is no specific diagnostic method, and it is a difficult lesion to diagnose before performing surgery. In order to improve clinicians’ understanding of a malignant-transformed mature teratoma and identification of malignant-transformed teratomas at early stages, we have reported a case of a mature teratoma with the malignant transformation of a sebaceous gland carcinoma in the Yiwu Central Hospital in Zhejiang, China on May 31, 2018. A review of the literature is also presented to summarize the clinical symptoms, pathogenesis, diagnosis, and treatment of malignant-transformed mature teratomas. Keywords: mature teratoma, malignant transformation, sebaceous gland carcinoma
{"title":"Malignant Transformation of a Mature Ovarian Teratoma: A Case Report and Review of the Literature","authors":"Ru-bing Hu, Yun-xiao Zhou, Xiao-qing Jin, Cheng-dong Chang, Hao Wang, Baomin Shi, Jingyun Shi","doi":"10.31491/CSRC.2018.9.022","DOIUrl":"https://doi.org/10.31491/CSRC.2018.9.022","url":null,"abstract":"A mature ovarian teratoma is a common benign germ cell tumor of the ovary in women that rarely causes a malignant transformation. However, a malignant transformation can occur in the ectodermal, mesodermal, and endodermal tissues of the tumor. A squamous cell carcinoma is the most common and an adenocarcinoma is the second most common malignant tumor derived from a cystic teratoma. Due to the rarity of malignant transformations in mature teratomas and the lack of clinical data, the clinical pathological features, malignant transformation mechanism, therapeutic methods, and prognosis-relevant factors are unclear. Currently, there is no specific diagnostic method, and it is a difficult lesion to diagnose before performing surgery. In order to improve clinicians’ understanding of a malignant-transformed mature teratoma and identification of malignant-transformed teratomas at early stages, we have reported a case of a mature teratoma with the malignant transformation of a sebaceous gland carcinoma in the Yiwu Central Hospital in Zhejiang, China on May 31, 2018. A review of the literature is also presented to summarize the clinical symptoms, pathogenesis, diagnosis, and treatment of malignant-transformed mature teratomas. Keywords: mature teratoma, malignant transformation, sebaceous gland carcinoma","PeriodicalId":158678,"journal":{"name":"Clinical Surgery Research Communications","volume":"41 26","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"113936734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-09-25DOI: 10.31491/CSRC.2018.9.018
Zhiliang Hu, Zhun He, Bo Jiang, Zhen Li, Tao Zhang
Objective : To investigate the clinical efficacy and value of uniportal video-assisted thoracoscopic bronchial sleeve lobectomy (BSL) in the treatment of central lung cancer. Methods : The clinical data of five patients who underwent uniportal video-assisted thoracoscopic BSL at our hospital from October 2014 to September 2016 were retrospectively analyzed. Results : The BSL procedure was successful in all five cases. The average operation time was 254 min, and the mean time to complete the anastomosis was 168 min. The average blood loss was 116 ml, and the mean number of lymph node dissections was 16.6/case. The average intubation time was 5 d, and the total postoperative drainage volume was 732 ml. The mean VAS score was 2.86, and the average postoperative hospital stay was 9.2 d. All patients were followed up for 3–19 months, and all of them survived without recurrence or metastasis. Conclusion : Uniportal video-assisted thoracoscopic BSL was found to be safe, reliable, and minimally invasive. Keywords : lung cancer; bronchial sleeve lobectomy; video-assisted thoracoscopic surgery; anastomosis
{"title":"Uniportal video-assisted thoracoscopic bronchial sleeve lobectomy in five patients","authors":"Zhiliang Hu, Zhun He, Bo Jiang, Zhen Li, Tao Zhang","doi":"10.31491/CSRC.2018.9.018","DOIUrl":"https://doi.org/10.31491/CSRC.2018.9.018","url":null,"abstract":"Objective : To investigate the clinical efficacy and value of uniportal video-assisted thoracoscopic bronchial sleeve lobectomy (BSL) in the treatment of central lung cancer. Methods : The clinical data of five patients who underwent uniportal video-assisted thoracoscopic BSL at our hospital from October 2014 to September 2016 were retrospectively analyzed. Results : The BSL procedure was successful in all five cases. The average operation time was 254 min, and the mean time to complete the anastomosis was 168 min. The average blood loss was 116 ml, and the mean number of lymph node dissections was 16.6/case. The average intubation time was 5 d, and the total postoperative drainage volume was 732 ml. The mean VAS score was 2.86, and the average postoperative hospital stay was 9.2 d. All patients were followed up for 3–19 months, and all of them survived without recurrence or metastasis. Conclusion : Uniportal video-assisted thoracoscopic BSL was found to be safe, reliable, and minimally invasive. Keywords : lung cancer; bronchial sleeve lobectomy; video-assisted thoracoscopic surgery; anastomosis","PeriodicalId":158678,"journal":{"name":"Clinical Surgery Research Communications","volume":"50 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121291651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-09-25DOI: 10.31491/CSRC.2018.9.020
Ling Zhang, Yaojun Lu, Lian-feng Zhang
Background : An increasing number of miRNAs were confirmed to be involved in the initiation and progression of colorectal cancer (CRC) by acting as cancer suppressor genes and oncogenes. The purpose of this study was to uncover the role of miR-449b in CRC and its underlying mechanisms. Methods : The expression profile of miR-449b in human CRC tissues and cell lines was determined using quantitative real-time polymerase chain reaction (qRT-PCR) analysis. The effect of miR-449b on CRC was assessed by CRC cell migration and invasion as determined using a transwell assay. A luciferase reporter assay was also carried out to explore the interaction between MMP2 3′UTR and miR-449b. Results : The current study revealed that the relative expression of miR-449b was decreased significantly in human CRC tissues and cell lines; meanwhile, miR-449b in metastatic CRC tissues was significantly lower than that in non-metastatic CRC tissues. We also observed that the forced miR-449b in CRC cells significantly restrained cell migration and invasion in vitro . In contrast to miR-449b, our study found that both the MMP-2 mRNA and protein levels were increased in CRC cells. MiR-449b negatively regulated MMP2 in CRC cells, and the result was corroborated by luciferase reporter assay. Most importantly, overexpression of MMP2 significantly reversed the miR-449b-mediated inhibition on the invasion and migration of CRC cells. Conclusion : Our data provided encouraging evidence that miR-449b possessed a strong inhibitory effect on CRC cell migration and invasion by negative regulation of MMP2. Keywords : Colorectal cancer; MiR-449b; MMP2; Migration; Invasion
{"title":"MiR-449b inhibits the migration and invasion of colorectal cancer cells through the negative regulation of MMP2","authors":"Ling Zhang, Yaojun Lu, Lian-feng Zhang","doi":"10.31491/CSRC.2018.9.020","DOIUrl":"https://doi.org/10.31491/CSRC.2018.9.020","url":null,"abstract":"Background : An increasing number of miRNAs were confirmed to be involved in the initiation and progression of colorectal cancer (CRC) by acting as cancer suppressor genes and oncogenes. The purpose of this study was to uncover the role of miR-449b in CRC and its underlying mechanisms. Methods : The expression profile of miR-449b in human CRC tissues and cell lines was determined using quantitative real-time polymerase chain reaction (qRT-PCR) analysis. The effect of miR-449b on CRC was assessed by CRC cell migration and invasion as determined using a transwell assay. A luciferase reporter assay was also carried out to explore the interaction between MMP2 3′UTR and miR-449b. Results : The current study revealed that the relative expression of miR-449b was decreased significantly in human CRC tissues and cell lines; meanwhile, miR-449b in metastatic CRC tissues was significantly lower than that in non-metastatic CRC tissues. We also observed that the forced miR-449b in CRC cells significantly restrained cell migration and invasion in vitro . In contrast to miR-449b, our study found that both the MMP-2 mRNA and protein levels were increased in CRC cells. MiR-449b negatively regulated MMP2 in CRC cells, and the result was corroborated by luciferase reporter assay. Most importantly, overexpression of MMP2 significantly reversed the miR-449b-mediated inhibition on the invasion and migration of CRC cells. Conclusion : Our data provided encouraging evidence that miR-449b possessed a strong inhibitory effect on CRC cell migration and invasion by negative regulation of MMP2. Keywords : Colorectal cancer; MiR-449b; MMP2; Migration; Invasion","PeriodicalId":158678,"journal":{"name":"Clinical Surgery Research Communications","volume":"37 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115337634","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-09-25DOI: 10.31491/CSRC.2018.9.021
Xiang Sun, Long-hua Luo, Kezhao Zhong, Jia-rui Li
Background LncRNA PCAT29 has been reported to play a role in the development of cancer; the role of FLOT1 in renal carcinoma has been also identified. This study aimed at exploring the interaction between them and their influence on the progression of renal carcinoma. M ethods The expression levels of lncRNA PCAT29 and the FLOT1 protein in tissues were determined separately using qRT-PCR and western blot, respectively. The endogenous expression of genes was modulated by recombinant plasmid and cell transfection. The cell viability, invasion, and migration were detected by MTT assay, transwell assay, and wound-healing cell migration assay, respectively. Any binding and interactions between the RNA and the proteins were determined with RNA immunoprecipitation and RNA pull-down assays. A nude mouse model for renal carcinoma was established for the i n vivo expression of PCAT29. R esults In renal carcinoma tissues, the expression of lncRNA PCAT29 was down-regulated while that of FLOT1 was up-regulated. PCAT29 negatively regulated FLOT1, and the overexpression of PCAT29 inhibited the cell proliferation viability, invasion, and migration of renal carcinoma by down-regulating FLOT1. The in vivo expression of PCAT29 inhibited tumor growth in a mouse model of renal carcinoma. C onclusion LncRNA PCAT29 inhibited the cell proliferation viability, invasion, and migration in renal carcinoma by down-regulating FLOT1, thereby suppressing tumor growth. Keywords : LncRNA PCAT29; renal carcinoma; cell proliferation; cell invasion and migration; FLOT1
{"title":"LncRNA PCAT29 suppresses cell proliferation, invasion, and migration in renal carcinoma by regulating FLOT1","authors":"Xiang Sun, Long-hua Luo, Kezhao Zhong, Jia-rui Li","doi":"10.31491/CSRC.2018.9.021","DOIUrl":"https://doi.org/10.31491/CSRC.2018.9.021","url":null,"abstract":"Background LncRNA PCAT29 has been reported to play a role in the development of cancer; the role of FLOT1 in renal carcinoma has been also identified. This study aimed at exploring the interaction between them and their influence on the progression of renal carcinoma. M ethods The expression levels of lncRNA PCAT29 and the FLOT1 protein in tissues were determined separately using qRT-PCR and western blot, respectively. The endogenous expression of genes was modulated by recombinant plasmid and cell transfection. The cell viability, invasion, and migration were detected by MTT assay, transwell assay, and wound-healing cell migration assay, respectively. Any binding and interactions between the RNA and the proteins were determined with RNA immunoprecipitation and RNA pull-down assays. A nude mouse model for renal carcinoma was established for the i n vivo expression of PCAT29. R esults In renal carcinoma tissues, the expression of lncRNA PCAT29 was down-regulated while that of FLOT1 was up-regulated. PCAT29 negatively regulated FLOT1, and the overexpression of PCAT29 inhibited the cell proliferation viability, invasion, and migration of renal carcinoma by down-regulating FLOT1. The in vivo expression of PCAT29 inhibited tumor growth in a mouse model of renal carcinoma. C onclusion LncRNA PCAT29 inhibited the cell proliferation viability, invasion, and migration in renal carcinoma by down-regulating FLOT1, thereby suppressing tumor growth. Keywords : LncRNA PCAT29; renal carcinoma; cell proliferation; cell invasion and migration; FLOT1","PeriodicalId":158678,"journal":{"name":"Clinical Surgery Research Communications","volume":"49 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127247897","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-06-26DOI: 10.31491/CSRC.2018.6.014
Wenqing Ge, Pan Hao, Yuhua Huang, Jianquan Hou, J. Pu, Liang-liang Wang
Objective: This study was conducted in order to investigate the anti-inflammatory effects of miR-539 on renal transplant ischemia-reperfusion (I/R) injury. Methods: A mouse model replicating renal transplant I/R injury and a cellular model of oxygen-glucose deprivation (OGD) treatment were established. The blood urea nitrogen (BUN) levels were determined for all models. The miR-539 expressions were detected in the kidney tissues and HK-2 cells following the different transfections using qRT-PCR. Western blotting was used to analyze myeloid differentiation factor 88 (MyD88), as well as the unphosphorylated and phosphorylated Nuclear factor-κB (NF-κB) protein expressions. The interactions between miR-539 and MyD88 were examined through a luciferase reporter assay. Moreover, pro-inflammatory cytokines levels, including the tumor necrosis factor (TNF)-α, interleukin (IL)-6, and IL-8, were measured using an enzyme-linked immunosorbent assay (ELISA). A renal tubular necrosis score (TNS) was employed as a means of assessing the renal function of mouse model. Results: miR-539 was downregulated during renal I/R injury. In vitro , miR-539 relieved the secretion of pro-inflammatory cytokines. A luciferase reporter assay demonstrated that MyD88 was a direct target of miR-539. Further investigation revealed that miR-539 inhibited I/R injury-induced inflammation by downregulating the MyD88/NF-κB pathway. It was shown that miR-539 exerted anti-inflammatory effects in the mice that underwent renal transplant I/R injury. Conclusion: MiR-539 alleviated inflammation in renal transplant I/R injury through the MyD88/NF-κB pathway.
{"title":"MiR-539 Inhibits Inflammation in Renal Transplant Iscemia-Reperfusion Injury Via Blocking the MyD88/NF-κB Pathway","authors":"Wenqing Ge, Pan Hao, Yuhua Huang, Jianquan Hou, J. Pu, Liang-liang Wang","doi":"10.31491/CSRC.2018.6.014","DOIUrl":"https://doi.org/10.31491/CSRC.2018.6.014","url":null,"abstract":"Objective: This study was conducted in order to investigate the anti-inflammatory effects of miR-539 on renal transplant ischemia-reperfusion (I/R) injury. Methods: A mouse model replicating renal transplant I/R injury and a cellular model of oxygen-glucose deprivation (OGD) treatment were established. The blood urea nitrogen (BUN) levels were determined for all models. The miR-539 expressions were detected in the kidney tissues and HK-2 cells following the different transfections using qRT-PCR. Western blotting was used to analyze myeloid differentiation factor 88 (MyD88), as well as the unphosphorylated and phosphorylated Nuclear factor-κB (NF-κB) protein expressions. The interactions between miR-539 and MyD88 were examined through a luciferase reporter assay. Moreover, pro-inflammatory cytokines levels, including the tumor necrosis factor (TNF)-α, interleukin (IL)-6, and IL-8, were measured using an enzyme-linked immunosorbent assay (ELISA). A renal tubular necrosis score (TNS) was employed as a means of assessing the renal function of mouse model. Results: miR-539 was downregulated during renal I/R injury. In vitro , miR-539 relieved the secretion of pro-inflammatory cytokines. A luciferase reporter assay demonstrated that MyD88 was a direct target of miR-539. Further investigation revealed that miR-539 inhibited I/R injury-induced inflammation by downregulating the MyD88/NF-κB pathway. It was shown that miR-539 exerted anti-inflammatory effects in the mice that underwent renal transplant I/R injury. Conclusion: MiR-539 alleviated inflammation in renal transplant I/R injury through the MyD88/NF-κB pathway.","PeriodicalId":158678,"journal":{"name":"Clinical Surgery Research Communications","volume":"55 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132004857","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-06-26DOI: 10.31491/CSRC.2018.6.013
Tuerhongjiang Abudourexiti, Sulaiman Reheman, Xiang-yu Meng, Liang Ma, Yunjiang Liu, C. He, B. Liang
Objective: The aim of this research was to evaluate the early rehabilitation and complications of percutaneous transforaminal endoscopic discectomies (PTEDs) in the treatment of lumbar disc herniations. Methods: From July 2015 to January 2017, ninety-one patients with lumbar disc herniations who underwent PTEDs were retrospectively enrolled. During the six month follow-up period, a visual analogue scale (VAS) and the Oswestry Disability Index (ODI) were used to evaluate the preoperative and postoperative (3 days, 3 months and 6 months) disturbance indexes. The Macnab criteria were used to evaluate the clinical efficacy. The operation time, intraoperative blood loss, average length of the hospital stay, postoperative recurrence rate, and complications were observed and recorded. Results: The VAS and ODI scores for the lumbocrural pain after the surgery were significantly lower than those before the surgery (p 0.05). The leg pain VAS score on the postoperative 3 rd day was significantly lower than that during the postoperative 3 rd month (p 0.05). Conclusion: Based on the results of this study, PTEDs for the treatment of lumbar disc herniations are safe and effective, with less trauma and complications. In addition, the postoperative rehabilitation time was markedly shortened.
{"title":"Percutaneous Transforaminal Endoscopic Discectomy in The Treatment of Lumbar Disc Herniation: Effects on Early Rehabilitation","authors":"Tuerhongjiang Abudourexiti, Sulaiman Reheman, Xiang-yu Meng, Liang Ma, Yunjiang Liu, C. He, B. Liang","doi":"10.31491/CSRC.2018.6.013","DOIUrl":"https://doi.org/10.31491/CSRC.2018.6.013","url":null,"abstract":"Objective: The aim of this research was to evaluate the early rehabilitation and complications of percutaneous transforaminal endoscopic discectomies (PTEDs) in the treatment of lumbar disc herniations. Methods: From July 2015 to January 2017, ninety-one patients with lumbar disc herniations who underwent PTEDs were retrospectively enrolled. During the six month follow-up period, a visual analogue scale (VAS) and the Oswestry Disability Index (ODI) were used to evaluate the preoperative and postoperative (3 days, 3 months and 6 months) disturbance indexes. The Macnab criteria were used to evaluate the clinical efficacy. The operation time, intraoperative blood loss, average length of the hospital stay, postoperative recurrence rate, and complications were observed and recorded. Results: The VAS and ODI scores for the lumbocrural pain after the surgery were significantly lower than those before the surgery (p 0.05). The leg pain VAS score on the postoperative 3 rd day was significantly lower than that during the postoperative 3 rd month (p 0.05). Conclusion: Based on the results of this study, PTEDs for the treatment of lumbar disc herniations are safe and effective, with less trauma and complications. In addition, the postoperative rehabilitation time was markedly shortened.","PeriodicalId":158678,"journal":{"name":"Clinical Surgery Research Communications","volume":"111 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127677404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-06-26DOI: 10.31491/CSRC.2018.6.012
Jun-feng Sun, Su-ling Wu, Guojun Wang, Jiaxiang Wang
Circular RNAs (circRNAs) are a class of non-coding RNAs (ncRNAs) that are involved in transcriptional and posttranscriptional gene expression regulation by sponging microRNAs (miRNAs). As miRNAs control a large set of biological processes, circRNA sponge activity also affects these pathways and thereby participates in human disease initiation and development. A growing number of works have confirmed that circRNAs may play critical roles in normal human tissues and organ functions and in the pathogenesis of human diseases, mainly by interacting with miRNAs. Herein, we review the expressions and functions of circRNAs in diverse disorders, including those of the nervous system and cardiovascular system, cancer, and other common diseases. As circRNAs are easily detected in many clinical samples, they present great potential as biomarkers for disease diagnosis and prognostic evaluation; thus, the prospect of using circRNAs as biomarkers for clinical decision-making is also discussed.
{"title":"Roles of Circular RNAs And Their Interactions With MicroRNAs in Human Disorders","authors":"Jun-feng Sun, Su-ling Wu, Guojun Wang, Jiaxiang Wang","doi":"10.31491/CSRC.2018.6.012","DOIUrl":"https://doi.org/10.31491/CSRC.2018.6.012","url":null,"abstract":"Circular RNAs (circRNAs) are a class of non-coding RNAs (ncRNAs) that are involved in transcriptional and posttranscriptional gene expression regulation by sponging microRNAs (miRNAs). As miRNAs control a large set of biological processes, circRNA sponge activity also affects these pathways and thereby participates in human disease initiation and development. A growing number of works have confirmed that circRNAs may play critical roles in normal human tissues and organ functions and in the pathogenesis of human diseases, mainly by interacting with miRNAs. Herein, we review the expressions and functions of circRNAs in diverse disorders, including those of the nervous system and cardiovascular system, cancer, and other common diseases. As circRNAs are easily detected in many clinical samples, they present great potential as biomarkers for disease diagnosis and prognostic evaluation; thus, the prospect of using circRNAs as biomarkers for clinical decision-making is also discussed.","PeriodicalId":158678,"journal":{"name":"Clinical Surgery Research Communications","volume":"70 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121282001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-06-26DOI: 10.31491/CSRC.2018.6.016
Ting Li, Xianguo Meng, W. Chen
Objective: This study was designed to evaluate the safety and short-term efficacy of a laparoscopic complete mesocolic excision (CME) in patients with right hemicolon cancer. Methods: A total of 88 patients with right hemicolon cancer were retrospectively reviewed. Forty patients underwent laparoscopic CMEs, and 48 patients underwent open CMEs. The clinical data were analyzed and the outcomes were compared between the two groups. Results: There was no significant difference between the laparoscopic CME group and open CME group with regard to the harvested lymph node number (16.60±3.20 vs. 15.33±3.10, respectively, P=0.060), hospital stay length (18.15±5.17 days vs. 17.21±4.47 days, respectively, P=0.360), and postoperative complications (10.0% vs. 10.4%, respectively, P=0.950). The operation time in the laparoscopic CME group (3.02±0.55 hours) was significantly longer than that in the open CME group (2.58±0.50 hours, P=0.004). The time to first flatus (3.28±0.75 days vs 3.92±0.71 days, respectively, P=0.001) and getting out of bed time (1.95±0.75 days vs 3.54±0.71 days, respectively, P=0.001) were both earlier in the laparoscopic CME group than in the open CME group. Conclusion: A laparoscopic CME is a safe and effective minimally invasive surgery for the treatment of right hemicolon cancer.
目的:本研究旨在评估腹腔镜下全肠系膜切除术(CME)治疗右半结肠癌患者的安全性和短期疗效。方法:对88例右半结肠癌患者的临床资料进行回顾性分析。40例患者行腹腔镜cme, 48例行开放式cme。分析两组临床资料，比较两组疗效。结果:腹腔镜CME组与开放式CME组在淋巴结清扫数(分别为16.60±3.20 vs 15.33±3.10,P=0.060)、住院时间(分别为18.15±5.17 d vs 17.21±4.47 d, P=0.360)、术后并发症(分别为10.0% vs 10.4%， P=0.950)方面差异无统计学意义。腹腔镜CME组手术时间(3.02±0.55 h)明显长于开放CME组(2.58±0.50 h, P=0.004)。第一次排气时间(分别为3.28±0.75天vs 3.92±0.71天，P=0.001)和下床时间(分别为1.95±0.75天vs 3.54±0.71天，P=0.001)腹腔镜CME组均早于开放式CME组。结论:腹腔镜下腔动脉造影是一种安全有效的治疗右半结肠癌的微创手术。
{"title":"Safety and Short-term Efcacy of a Laparoscopic Complete Mesocolic Excision for the Surgical Treatment of Right Hemicolon Cancer","authors":"Ting Li, Xianguo Meng, W. Chen","doi":"10.31491/CSRC.2018.6.016","DOIUrl":"https://doi.org/10.31491/CSRC.2018.6.016","url":null,"abstract":"Objective: This study was designed to evaluate the safety and short-term efficacy of a laparoscopic complete mesocolic excision (CME) in patients with right hemicolon cancer. Methods: A total of 88 patients with right hemicolon cancer were retrospectively reviewed. Forty patients underwent laparoscopic CMEs, and 48 patients underwent open CMEs. The clinical data were analyzed and the outcomes were compared between the two groups. Results: There was no significant difference between the laparoscopic CME group and open CME group with regard to the harvested lymph node number (16.60±3.20 vs. 15.33±3.10, respectively, P=0.060), hospital stay length (18.15±5.17 days vs. 17.21±4.47 days, respectively, P=0.360), and postoperative complications (10.0% vs. 10.4%, respectively, P=0.950). The operation time in the laparoscopic CME group (3.02±0.55 hours) was significantly longer than that in the open CME group (2.58±0.50 hours, P=0.004). The time to first flatus (3.28±0.75 days vs 3.92±0.71 days, respectively, P=0.001) and getting out of bed time (1.95±0.75 days vs 3.54±0.71 days, respectively, P=0.001) were both earlier in the laparoscopic CME group than in the open CME group. Conclusion: A laparoscopic CME is a safe and effective minimally invasive surgery for the treatment of right hemicolon cancer.","PeriodicalId":158678,"journal":{"name":"Clinical Surgery Research Communications","volume":"05 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129393627","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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