首页 > 最新文献

Journal of Gastroenterology and Hepatology最新文献

英文 中文
Efficacy of Magnifying Texture and Color Enhancement Imaging With Indigo Carmine as an Alternative to Crystal Violet for Colorectal Tumors. 靛蓝胭脂红替代结晶紫对结直肠肿瘤的放大纹理和彩色增强成像效果。
IF 3.7 3区 医学 Q2 GASTROENTEROLOGY & HEPATOLOGY Pub Date : 2025-01-14 DOI: 10.1111/jgh.16886
Tsubasa Ishikawa, Kenichiro Okimoto, Tomoaki Matsumura, Naoki Akizue, Akane Kurosugi, Michiko Sonoda, Tatsuya Kaneko, Yuki Ohta, Takashi Taida, Keisuke Matsusaka, Jun Kato, Jun-Ichiro Ikeda, Naoya Kato

Background: Pit pattern diagnosis using crystal violet staining for colorectal tumors is useful for qualitative and depth diagnosis. However, due to its reported carcinogenic potential, the use of crystal violet has been restricted. This study was aimed at investigating the efficacy of texture and color enhancement imaging (TXI) magnification in pit pattern diagnosis.

Methods: This study enrolled consecutive patients with consent obtained and with colonic tumors indicated for magnifying endoscopy in which all modalities' images (magnified observation with crystal violet staining (CV), magnified white light observation with indigo carmine (IC-WLI), and magnified TXI observation with indigo carmine (IC-TXI)) were evaluable between July 2020 and January 2023. The visibility of the pit pattern using a 5-point Likert scale and its diagnostic accuracy were compared (IC-TXI vs. IC-WLI, reference: CV, by three experts).

Results: A total of 145 colorectal tumors from 145 patients were included. Visibility scores for the pit pattern were significantly higher with IC-TXI compared to IC-WLI (all three experts, p < 0.0001). The pit pattern match rate (Type II/III/IV/V) of IC-TXI compared to CV was also superior to IC-WLI (72.9% vs. 59.7%; p = 0.02).

Conclusions: IC-TXI provided reasonably good and higher visibility and diagnostic accuracy than IC-WLI for pit pattern diagnosis of colorectal tumors compared to CV, suggesting it as an alternative to CV.

背景:结晶紫染色对结直肠肿瘤的坑型诊断有助于定性和深度诊断。然而,由于其潜在的致癌性,水晶紫的使用受到限制。本研究旨在探讨纹理和彩色增强成像(TXI)放大在坑纹诊断中的作用。方法:本研究招募了连续的获得同意的结肠肿瘤患者,在2020年7月至2023年1月期间,所有模式的图像(结晶紫染色放大观察(CV),靛蓝胭脂红放大白光观察(IC-WLI)和靛蓝胭脂红放大TXI观察(IC-TXI))都是可评估的。使用5点李克特量表比较凹坑模式的可见性及其诊断准确性(IC-TXI与IC-WLI,参考文献:CV,由三位专家)。结果:145例患者共纳入145例结直肠肿瘤。与IC-WLI相比,IC-TXI的窝型可见性评分明显更高(三位专家,p)。结论:与CV相比,IC-TXI在结肠肿瘤窝型诊断方面提供了相当好的可见性和更高的诊断准确性,表明IC-TXI可以替代CV。
{"title":"Efficacy of Magnifying Texture and Color Enhancement Imaging With Indigo Carmine as an Alternative to Crystal Violet for Colorectal Tumors.","authors":"Tsubasa Ishikawa, Kenichiro Okimoto, Tomoaki Matsumura, Naoki Akizue, Akane Kurosugi, Michiko Sonoda, Tatsuya Kaneko, Yuki Ohta, Takashi Taida, Keisuke Matsusaka, Jun Kato, Jun-Ichiro Ikeda, Naoya Kato","doi":"10.1111/jgh.16886","DOIUrl":"https://doi.org/10.1111/jgh.16886","url":null,"abstract":"<p><strong>Background: </strong>Pit pattern diagnosis using crystal violet staining for colorectal tumors is useful for qualitative and depth diagnosis. However, due to its reported carcinogenic potential, the use of crystal violet has been restricted. This study was aimed at investigating the efficacy of texture and color enhancement imaging (TXI) magnification in pit pattern diagnosis.</p><p><strong>Methods: </strong>This study enrolled consecutive patients with consent obtained and with colonic tumors indicated for magnifying endoscopy in which all modalities' images (magnified observation with crystal violet staining (CV), magnified white light observation with indigo carmine (IC-WLI), and magnified TXI observation with indigo carmine (IC-TXI)) were evaluable between July 2020 and January 2023. The visibility of the pit pattern using a 5-point Likert scale and its diagnostic accuracy were compared (IC-TXI vs. IC-WLI, reference: CV, by three experts).</p><p><strong>Results: </strong>A total of 145 colorectal tumors from 145 patients were included. Visibility scores for the pit pattern were significantly higher with IC-TXI compared to IC-WLI (all three experts, p < 0.0001). The pit pattern match rate (Type II/III/IV/V) of IC-TXI compared to CV was also superior to IC-WLI (72.9% vs. 59.7%; p = 0.02).</p><p><strong>Conclusions: </strong>IC-TXI provided reasonably good and higher visibility and diagnostic accuracy than IC-WLI for pit pattern diagnosis of colorectal tumors compared to CV, suggesting it as an alternative to CV.</p>","PeriodicalId":15877,"journal":{"name":"Journal of Gastroenterology and Hepatology","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142983769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Clonorchis sinensis Promotes Intrahepatic Cholangiocarcinoma Progression by Activating FASN-Mediated Fatty Acid Metabolism. 华支睾吸虫通过激活fasn介导的脂肪酸代谢促进肝内胆管癌进展。
IF 3.7 3区 医学 Q2 GASTROENTEROLOGY & HEPATOLOGY Pub Date : 2025-01-13 DOI: 10.1111/jgh.16879
Xiaoxue Ren, Yanqing Wu, Tongtong Song, Qingxia Yang, Qianying Zhou, Jie Lin, Lixia Xu, Bangde Xiang, Zebin Chen, Ying Zhang

Background: Clonorchis sinensis infection is an important risk factor for intrahepatic cholangiocarcinoma (ICC). C. sinensis positive (C.s+) ICC patients had much shorter overall survival (OS) compared with C. sinensis negative (C.s-) group. This study aims to explore the impact and underlying mechanism of C. sinensis infection on ICC progression.

Methods: In this study, ICC patients underwent surgery from two medical centers enrolled. RNA sequencing was used to determine the downstream activated pathways and genes. Furthermore, we demonstrated the potential mechanism of C. sinensis infection in promoting ICC progression through in vitro co culture systems and two animal models.

Results: Through RNA sequencing, we found fatty acid metabolism and the expression of fatty acid synthase (FASN), a key enzyme catalyzing long-chain fatty acid synthesis, were significantly elevated in C.s+ ICCs. Then, we found excretory/secretory products (ESPs) secreted by C. sinensis could significantly upregulate the expression of transcription factor E2F1, thereby promoting FASN expression and fatty acid synthesis in tumor cells, which ultimately accelerating tumor progression. However, the promotive effect disappeared when FASN was knocked down. Meanwhile, ESPs could promote tumor growth, increasing FASN expression and free fatty acid level in both subcutaneous and orthotopic mouse models.

Conclusion: This study indicates that C. sinensis infection could upregulate the level of FASN and activate fatty acid synthesis pathway, thereby accelerating ICC progression. This provides a new insight for the clinical treatment of ICC with C. sinensis infection.

背景:华支睾吸虫感染是肝内胆管癌(ICC)的重要危险因素。与C.s-组相比,C.s+组ICC患者总生存期(OS)明显缩短。本研究旨在探讨中华梭菌感染对ICC进展的影响及其机制。方法:本研究纳入了两家医疗中心的ICC患者。RNA测序用于确定下游激活途径和基因。此外,我们通过体外共培养系统和两种动物模型证明了中华梭菌感染促进ICC进展的潜在机制。结果:通过RNA测序,我们发现C.s+ ICCs的脂肪酸代谢和催化长链脂肪酸合成的关键酶脂肪酸合成酶(FASN)的表达显著升高。然后,我们发现中华葡萄球菌分泌的排泄/分泌产物(ESPs)可以显著上调转录因子E2F1的表达,从而促进肿瘤细胞中FASN的表达和脂肪酸的合成,最终加速肿瘤的进展。而FASN被敲除后,其促进作用消失。同时,在皮下和原位小鼠模型中,ESPs均能促进肿瘤生长,增加FASN表达和游离脂肪酸水平。结论:本研究提示中华梭菌感染可上调FASN水平,激活脂肪酸合成途径,从而加速ICC的进展。这为临床治疗伴有中华梭菌感染的ICC提供了新的思路。
{"title":"Clonorchis sinensis Promotes Intrahepatic Cholangiocarcinoma Progression by Activating FASN-Mediated Fatty Acid Metabolism.","authors":"Xiaoxue Ren, Yanqing Wu, Tongtong Song, Qingxia Yang, Qianying Zhou, Jie Lin, Lixia Xu, Bangde Xiang, Zebin Chen, Ying Zhang","doi":"10.1111/jgh.16879","DOIUrl":"https://doi.org/10.1111/jgh.16879","url":null,"abstract":"<p><strong>Background: </strong>Clonorchis sinensis infection is an important risk factor for intrahepatic cholangiocarcinoma (ICC). C. sinensis positive (C.s+) ICC patients had much shorter overall survival (OS) compared with C. sinensis negative (C.s-) group. This study aims to explore the impact and underlying mechanism of C. sinensis infection on ICC progression.</p><p><strong>Methods: </strong>In this study, ICC patients underwent surgery from two medical centers enrolled. RNA sequencing was used to determine the downstream activated pathways and genes. Furthermore, we demonstrated the potential mechanism of C. sinensis infection in promoting ICC progression through in vitro co culture systems and two animal models.</p><p><strong>Results: </strong>Through RNA sequencing, we found fatty acid metabolism and the expression of fatty acid synthase (FASN), a key enzyme catalyzing long-chain fatty acid synthesis, were significantly elevated in C.s+ ICCs. Then, we found excretory/secretory products (ESPs) secreted by C. sinensis could significantly upregulate the expression of transcription factor E2F1, thereby promoting FASN expression and fatty acid synthesis in tumor cells, which ultimately accelerating tumor progression. However, the promotive effect disappeared when FASN was knocked down. Meanwhile, ESPs could promote tumor growth, increasing FASN expression and free fatty acid level in both subcutaneous and orthotopic mouse models.</p><p><strong>Conclusion: </strong>This study indicates that C. sinensis infection could upregulate the level of FASN and activate fatty acid synthesis pathway, thereby accelerating ICC progression. This provides a new insight for the clinical treatment of ICC with C. sinensis infection.</p>","PeriodicalId":15877,"journal":{"name":"Journal of Gastroenterology and Hepatology","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142978736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Successful Long-Term Outcomes After Combined Endoscopic Stricturotomy and Lumen-Apposing Metal Stent in Crohn's Disease With Primary Ileocecal Valve Stricture. 内镜下狭窄切除术联合置管金属支架治疗克罗恩病合并回盲瓣狭窄的远期疗效。
IF 3.7 3区 医学 Q2 GASTROENTEROLOGY & HEPATOLOGY Pub Date : 2025-01-13 DOI: 10.1111/jgh.16887
Ilhami Yuksel, Oguz Ozturk, Yavuz Cagir, Muhammed Bahaddin Durak
{"title":"Successful Long-Term Outcomes After Combined Endoscopic Stricturotomy and Lumen-Apposing Metal Stent in Crohn's Disease With Primary Ileocecal Valve Stricture.","authors":"Ilhami Yuksel, Oguz Ozturk, Yavuz Cagir, Muhammed Bahaddin Durak","doi":"10.1111/jgh.16887","DOIUrl":"https://doi.org/10.1111/jgh.16887","url":null,"abstract":"","PeriodicalId":15877,"journal":{"name":"Journal of Gastroenterology and Hepatology","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142978751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Characterization of NK Cells Using Single-Cell RNA Sequencing in Patients With Acute-On-Chronic Liver Failure. 使用单细胞RNA测序在急性慢性肝衰竭患者中表征NK细胞。
IF 3.7 3区 医学 Q2 GASTROENTEROLOGY & HEPATOLOGY Pub Date : 2025-01-12 DOI: 10.1111/jgh.16870
Ninghui Zhao, Han Wang, Miaoxin Zhang, Wei Tian, Yulong Liu, Dean Tian, Jia Yao, Mei Liu

Background and aim: Acute-on-chronic liver failure (ACLF) is characterized by fast progression and high mortality, with systemic inflammation and immune paralysis as its key events. While natural killer (NK) cells are key innate immune cells, their unique function and subpopulation heterogeneity in ACLF have not been fully elucidated. This study aimed to investigate the characteristics of NK cell subsets in the peripheral blood of patients with ACLF and determine their roles in the inflammatory responses.

Methods: Circulating NK cells (14 751 cells) from patients with ACLF and healthy controls (HCs) were subjected to single-cell RNA sequencing (scRNA-seq). Clustering and annotation were used to identify the features of NK cell subsets and the characteristics of disease progression in ACLF.

Results: Four NK cell subsets were obtained, including adaptive NK cells, mature NK cells, inflamed NK cells, and CD56bright NK cells. Compared with the HCs, the patients with ACLF had a significantly lower proportion of Mature NK cells and a higher proportion of Inflamed NK cells. Quasi-temporal analysis showed that Inflamed NK cells were highly enriched in the late quasi-temporal sequence, and genes related to pro-inflammatory were significantly up-regulated in Inflamed NK cells. In addition, scRNA-seq and flow cytometry confirmed that the expression level of cell migration inducing hyaluronidase 2 (CEMIP2) in NK cells progressively increased from the HC group to the ACLF survival group and then to the ACLF death group.

Conclusions: scRNA-seq reveals that Inflamed NK cell subsets are associated with ACLF progression and poor prognosis. CEMIP2 may be a molecular marker for ACLF progression.

背景与目的:急性慢性肝衰竭(ACLF)具有进展快、死亡率高的特点,全身性炎症和免疫瘫痪是其关键事件。虽然自然杀伤细胞(NK)是关键的先天免疫细胞,但其在ACLF中的独特功能和亚群异质性尚未完全阐明。本研究旨在探讨ACLF患者外周血NK细胞亚群的特征,并确定其在炎症反应中的作用。方法:对ACLF患者和健康对照(hc)外周血NK细胞(14751个细胞)进行单细胞RNA测序(scRNA-seq)。采用聚类和注释的方法鉴定NK细胞亚群的特征和ACLF的疾病进展特征。结果:获得4个NK细胞亚群,包括适应性NK细胞、成熟NK细胞、炎症NK细胞和CD56bright NK细胞。与hc相比,ACLF患者的成熟NK细胞比例明显降低,炎症NK细胞比例明显升高。准时间序列分析显示,炎症NK细胞在准时间序列晚期高度富集,促炎相关基因在炎症NK细胞中显著上调。此外,scRNA-seq和流式细胞术证实NK细胞中细胞迁移诱导透明质酸酶2 (CEMIP2)的表达水平从HC组到ACLF存活组再到ACLF死亡组逐渐升高。结论:scRNA-seq显示炎症NK细胞亚群与ACLF进展和不良预后相关。CEMIP2可能是ACLF进展的分子标志物。
{"title":"Characterization of NK Cells Using Single-Cell RNA Sequencing in Patients With Acute-On-Chronic Liver Failure.","authors":"Ninghui Zhao, Han Wang, Miaoxin Zhang, Wei Tian, Yulong Liu, Dean Tian, Jia Yao, Mei Liu","doi":"10.1111/jgh.16870","DOIUrl":"https://doi.org/10.1111/jgh.16870","url":null,"abstract":"<p><strong>Background and aim: </strong>Acute-on-chronic liver failure (ACLF) is characterized by fast progression and high mortality, with systemic inflammation and immune paralysis as its key events. While natural killer (NK) cells are key innate immune cells, their unique function and subpopulation heterogeneity in ACLF have not been fully elucidated. This study aimed to investigate the characteristics of NK cell subsets in the peripheral blood of patients with ACLF and determine their roles in the inflammatory responses.</p><p><strong>Methods: </strong>Circulating NK cells (14 751 cells) from patients with ACLF and healthy controls (HCs) were subjected to single-cell RNA sequencing (scRNA-seq). Clustering and annotation were used to identify the features of NK cell subsets and the characteristics of disease progression in ACLF.</p><p><strong>Results: </strong>Four NK cell subsets were obtained, including adaptive NK cells, mature NK cells, inflamed NK cells, and CD56<sup>bright</sup> NK cells. Compared with the HCs, the patients with ACLF had a significantly lower proportion of Mature NK cells and a higher proportion of Inflamed NK cells. Quasi-temporal analysis showed that Inflamed NK cells were highly enriched in the late quasi-temporal sequence, and genes related to pro-inflammatory were significantly up-regulated in Inflamed NK cells. In addition, scRNA-seq and flow cytometry confirmed that the expression level of cell migration inducing hyaluronidase 2 (CEMIP2) in NK cells progressively increased from the HC group to the ACLF survival group and then to the ACLF death group.</p><p><strong>Conclusions: </strong>scRNA-seq reveals that Inflamed NK cell subsets are associated with ACLF progression and poor prognosis. CEMIP2 may be a molecular marker for ACLF progression.</p>","PeriodicalId":15877,"journal":{"name":"Journal of Gastroenterology and Hepatology","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-01-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142971025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The Effects of 5-Aminosalicylic Acid on Janus Kinase Inhibitor Treatment in Ulcerative Colitis. 5-氨基水杨酸对溃疡性结肠炎Janus激酶抑制剂治疗的影响。
IF 3.7 3区 医学 Q2 GASTROENTEROLOGY & HEPATOLOGY Pub Date : 2025-01-11 DOI: 10.1111/jgh.16867
Cong Dai, Yu-Hong Huang
{"title":"The Effects of 5-Aminosalicylic Acid on Janus Kinase Inhibitor Treatment in Ulcerative Colitis.","authors":"Cong Dai, Yu-Hong Huang","doi":"10.1111/jgh.16867","DOIUrl":"https://doi.org/10.1111/jgh.16867","url":null,"abstract":"","PeriodicalId":15877,"journal":{"name":"Journal of Gastroenterology and Hepatology","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142965515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Analysis of Immunometabolic Profiles in Patients With Chronic Drug-Induced Liver Injury and Validation in Mice to Reveal Potential Mechanisms. 慢性药物性肝损伤患者免疫代谢谱分析及小鼠验证揭示潜在机制
IF 3.7 3区 医学 Q2 GASTROENTEROLOGY & HEPATOLOGY Pub Date : 2025-01-11 DOI: 10.1111/jgh.16876
Xingran Zhai, Xian He, Ang Huang, Zherui Liu, Shaoting Chen, Binxia Chang, Yun Zhu, Huan Xie, Zhaofang Bai, Xiaohe Xiao, Ying Sun, Jiabo Wang, Yawen Lu, Zhengsheng Zou

Background: The mechanism underlying chronic drug-induced liver injury (DILI) remains unclear. Immune activation is a common feature of DILI progression and is closely associated with metabolism. We explored the immunometabolic profile of chronic DILI and the potential mechanism of chronic DILI progression.

Methods: Plasma and peripheral blood mononuclear cells from patients with chronic DILI were analyzed using multiplex immunoassays and untargeted metabolomics to reveal their immunometabolic profile. The effects and potential mechanisms of chronic DILI-related metabolite on acute or chronic liver injury induced by LPS or CCl4 in mice were investigated.

Results: Patients with chronic DILI exhibited elevated plasma IL-6, IL-12p70, IL-15 and reduced IL-10 levels. The percentage of IL-12+ monocytes was higher, while that of CD206+ monocytes, IL-10+ monocytes, Th2, Treg, and IL-10+ CD4+ T cells were lower in patients with chronic DILI compared to those with acute DILI. We identified the most significantly increased metabolite in patients with chronic DILI was cis-aconitic acid (CAA). Administration of CAA can attenuate liver injury in mice with acute liver injury induced by LPS or CCl4 and promote the spontaneous resolution of liver fibrosis in mice with chronic live injury induced by CCl4. The protective mechanism of CAA against liver injury is associated with the inhibition of hepatic macrophage infiltration and polarization, which is achieved by inhibiting the secretion of neutrophil-derived IL-33 and subsequent phosphorylation of GATA3.

Conclusions: CAA, which is elevated in patients with chronic DILI, protects against liver injury by inhibiting hepatic macrophage infiltration and polarization through the suppression of the IL-33/GATA3 pathway, suggesting that CAA may serve as a potential target for regulating tissue repair in liver injury.

背景:慢性药物性肝损伤(DILI)的机制尚不清楚。免疫激活是DILI进展的共同特征,与代谢密切相关。我们探讨了慢性DILI的免疫代谢特征和慢性DILI进展的潜在机制。方法:采用多重免疫分析和非靶向代谢组学分析慢性DILI患者血浆和外周血单个核细胞,揭示其免疫代谢谱。研究慢性dili相关代谢物在LPS或CCl4诱导小鼠急性或慢性肝损伤中的作用及其可能机制。结果:慢性DILI患者血浆IL-6、IL-12p70、IL-15水平升高,IL-10水平降低。慢性DILI患者的IL-12+单核细胞比例高于急性DILI患者,而CD206+单核细胞、IL-10+单核细胞、Th2、Treg和IL-10+ CD4+ T细胞比例低于急性DILI患者。我们发现慢性DILI患者中增加最显著的代谢物是顺式乌头酸(CAA)。给药CAA可减轻LPS或CCl4诱导的急性肝损伤小鼠的肝损伤,促进CCl4诱导的慢性活损伤小鼠肝纤维化的自发消退。CAA对肝损伤的保护机制与抑制肝巨噬细胞浸润和极化有关,这是通过抑制中性粒细胞源性IL-33的分泌和随后的GATA3磷酸化来实现的。结论:CAA在慢性DILI患者中升高,通过抑制IL-33/GATA3通路抑制肝巨噬细胞浸润和极化,对肝损伤具有保护作用,提示CAA可能是调节肝损伤组织修复的潜在靶点。
{"title":"Analysis of Immunometabolic Profiles in Patients With Chronic Drug-Induced Liver Injury and Validation in Mice to Reveal Potential Mechanisms.","authors":"Xingran Zhai, Xian He, Ang Huang, Zherui Liu, Shaoting Chen, Binxia Chang, Yun Zhu, Huan Xie, Zhaofang Bai, Xiaohe Xiao, Ying Sun, Jiabo Wang, Yawen Lu, Zhengsheng Zou","doi":"10.1111/jgh.16876","DOIUrl":"https://doi.org/10.1111/jgh.16876","url":null,"abstract":"<p><strong>Background: </strong>The mechanism underlying chronic drug-induced liver injury (DILI) remains unclear. Immune activation is a common feature of DILI progression and is closely associated with metabolism. We explored the immunometabolic profile of chronic DILI and the potential mechanism of chronic DILI progression.</p><p><strong>Methods: </strong>Plasma and peripheral blood mononuclear cells from patients with chronic DILI were analyzed using multiplex immunoassays and untargeted metabolomics to reveal their immunometabolic profile. The effects and potential mechanisms of chronic DILI-related metabolite on acute or chronic liver injury induced by LPS or CCl<sub>4</sub> in mice were investigated.</p><p><strong>Results: </strong>Patients with chronic DILI exhibited elevated plasma IL-6, IL-12p70, IL-15 and reduced IL-10 levels. The percentage of IL-12<sup>+</sup> monocytes was higher, while that of CD206<sup>+</sup> monocytes, IL-10<sup>+</sup> monocytes, Th2, Treg, and IL-10<sup>+</sup> CD4<sup>+</sup> T cells were lower in patients with chronic DILI compared to those with acute DILI. We identified the most significantly increased metabolite in patients with chronic DILI was cis-aconitic acid (CAA). Administration of CAA can attenuate liver injury in mice with acute liver injury induced by LPS or CCl<sub>4</sub> and promote the spontaneous resolution of liver fibrosis in mice with chronic live injury induced by CCl<sub>4</sub>. The protective mechanism of CAA against liver injury is associated with the inhibition of hepatic macrophage infiltration and polarization, which is achieved by inhibiting the secretion of neutrophil-derived IL-33 and subsequent phosphorylation of GATA3.</p><p><strong>Conclusions: </strong>CAA, which is elevated in patients with chronic DILI, protects against liver injury by inhibiting hepatic macrophage infiltration and polarization through the suppression of the IL-33/GATA3 pathway, suggesting that CAA may serve as a potential target for regulating tissue repair in liver injury.</p>","PeriodicalId":15877,"journal":{"name":"Journal of Gastroenterology and Hepatology","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142965510","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Lynch Syndrome: Similarities and Differences of Recommendations in Published Guidelines. 林奇综合征:已出版指南中建议的异同。
IF 3.7 3区 医学 Q2 GASTROENTEROLOGY & HEPATOLOGY Pub Date : 2025-01-11 DOI: 10.1111/jgh.16881
Naim Abu-Freha, Wael Hozaeel, Sarah Weissmann, Zlata Lerner, Liza Ben-Shoshan, Roba Ganayem, Lior H Katz

Background: In this review, we aimed to compare the recommendations for Lynch syndrome (LS).

Methods: We compared the LS's guidelines of different medical societies, including recommendations for cancer surveillance, aspirin treatment, and universal screening.

Results: Most guidelines for LS patients recommend intervals of 1-2 years for performing colonoscopy, though there is disagreement regarding the age to begin CRC screening (dependent on status as a MLH1/MSH2 or MSH6/PMS2 carrier). There are inconsistencies between LS guidelines for gastric cancer surveillance. Most guidelines do not recommend routine surveillance of the pancreas and small bowel. Most but not all of the guidelines support endometrial and ovarian surveillance with transvaginal ultrasound and endometrial biopsy. Only two societies recommend urological surveillance, while others recommend surveillance among high-risk carriers with family history only. There is significant disagreement between the guidelines about the recommendation for limited or extended bowel resection among patients with CRC. Aspirin use is recommended by most societies, though some with reservations, and most of them recommend universal screening.

Conclusions: There are significant disparities and disagreements in the guidelines and recommendations for patients with LS, causing confusion and difficulties for clinicians. Harmonization and cooperation are needed between the societies creating LS guidelines.

背景:在这篇综述中,我们旨在比较Lynch综合征(LS)的推荐治疗方法。方法:我们比较了不同医学协会的LS指南,包括对癌症监测、阿司匹林治疗和普遍筛查的建议。结果:大多数LS患者指南建议结肠镜检查间隔1-2年,尽管对于开始CRC筛查的年龄存在分歧(取决于MLH1/MSH2或MSH6/PMS2携带者的状态)。胃癌监测的LS指南之间存在不一致。大多数指南不建议对胰腺和小肠进行常规监测。大多数但不是全部的指南支持子宫内膜和卵巢监测经阴道超声和子宫内膜活检。只有两个学会建议进行泌尿系统监测,而其他学会则建议仅对有家族史的高危携带者进行监测。指南中对于CRC患者推荐有限或延长的肠切除术存在明显的分歧。大多数社会都建议使用阿司匹林,尽管有些社会有所保留,但大多数社会都建议进行普遍筛查。结论:针对LS患者的指南和建议存在显著差异和分歧,给临床医生带来困惑和困难。制定LS准则的社会之间需要协调和合作。
{"title":"Lynch Syndrome: Similarities and Differences of Recommendations in Published Guidelines.","authors":"Naim Abu-Freha, Wael Hozaeel, Sarah Weissmann, Zlata Lerner, Liza Ben-Shoshan, Roba Ganayem, Lior H Katz","doi":"10.1111/jgh.16881","DOIUrl":"https://doi.org/10.1111/jgh.16881","url":null,"abstract":"<p><strong>Background: </strong>In this review, we aimed to compare the recommendations for Lynch syndrome (LS).</p><p><strong>Methods: </strong>We compared the LS's guidelines of different medical societies, including recommendations for cancer surveillance, aspirin treatment, and universal screening.</p><p><strong>Results: </strong>Most guidelines for LS patients recommend intervals of 1-2 years for performing colonoscopy, though there is disagreement regarding the age to begin CRC screening (dependent on status as a MLH1/MSH2 or MSH6/PMS2 carrier). There are inconsistencies between LS guidelines for gastric cancer surveillance. Most guidelines do not recommend routine surveillance of the pancreas and small bowel. Most but not all of the guidelines support endometrial and ovarian surveillance with transvaginal ultrasound and endometrial biopsy. Only two societies recommend urological surveillance, while others recommend surveillance among high-risk carriers with family history only. There is significant disagreement between the guidelines about the recommendation for limited or extended bowel resection among patients with CRC. Aspirin use is recommended by most societies, though some with reservations, and most of them recommend universal screening.</p><p><strong>Conclusions: </strong>There are significant disparities and disagreements in the guidelines and recommendations for patients with LS, causing confusion and difficulties for clinicians. Harmonization and cooperation are needed between the societies creating LS guidelines.</p>","PeriodicalId":15877,"journal":{"name":"Journal of Gastroenterology and Hepatology","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142965513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Global Inequalities in the Burden of Gallbladder and Biliary Tract Cancer From 1990 to 2021: Findings From the Global Burden of Disease Study 2021. 1990年至2021年胆囊和胆道癌症负担的全球不平等:来自2021年全球疾病负担研究的结果
IF 3.7 3区 医学 Q2 GASTROENTEROLOGY & HEPATOLOGY Pub Date : 2025-01-09 DOI: 10.1111/jgh.16866
Chunlong Liu, Jiangtao Yu
{"title":"Global Inequalities in the Burden of Gallbladder and Biliary Tract Cancer From 1990 to 2021: Findings From the Global Burden of Disease Study 2021.","authors":"Chunlong Liu, Jiangtao Yu","doi":"10.1111/jgh.16866","DOIUrl":"https://doi.org/10.1111/jgh.16866","url":null,"abstract":"","PeriodicalId":15877,"journal":{"name":"Journal of Gastroenterology and Hepatology","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142950014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effects of Different Bowel Preparation Regimens and Age Factors on the Gut Microbiota: A Prospective Randomized Controlled Study. 不同肠道准备方案和年龄因素对肠道微生物群的影响:一项前瞻性随机对照研究。
IF 3.7 3区 医学 Q2 GASTROENTEROLOGY & HEPATOLOGY Pub Date : 2025-01-08 DOI: 10.1111/jgh.16868
Yuhang Zhou, Huihui Ji, Shuang Zhang, Xiangrong Zhang, Junhai Zhang, Yujing Wang, Huifen Wang, Yanli Zhang, Shiyu Du

Background: Opinions about the impact of bowel preparation on the gut microbiota are divided. This study investigated the effects of different regimens on the gut microbiota post-bowel preparation and the differences in responses across different age groups.

Methods: This single-center, prospective, randomized, controlled clinical trial included 194 patients. Patients were categorized into two groups: one group receiving polyethylene glycol (n = 108) and one receiving sodium picosulfate (n = 86) for bowel preparation. Fecal samples were collected at baseline and on days 7 and 14 post-bowel preparation. The microbiota's diversity and composition were analyzed using 16S ribosomal RNA gene sequencing, followed by comparative analyses.

Results: The gut microbiota's abundance and diversity in patients significantly decreased post-bowel preparation, which did not recover to the level of pre-bowel preparation on Day 14. When comparing different regimens, the polyethylene glycol and sodium picosulfate groups recovered faster in richness and diversity, respectively. Patients aged < 65 years had higher richness and diversity of the gut microbiota, whereas the microbiota structure in those aged ≥ 65 years returned to the baseline state faster. The structure of beta diversity is significantly altered and did not return in the short term. However, in the elderly population aged ≥ 65 years, it can rebound quickly. This study also identified a number of significantly altered bacterial genera.

Conclusions: Following the use of different bowel preparation regimens, the gut microbiota recovers in diverse ways, with older people over 65 experiencing a faster recovery of the microbial structure.

背景:关于肠道准备对肠道微生物群的影响,意见不一。本研究调查了不同方案对肠道准备后肠道微生物群的影响,以及不同年龄组的反应差异。方法:本研究为单中心、前瞻性、随机对照临床试验,纳入194例患者。患者分为两组:一组接受聚乙二醇(n = 108),另一组接受pico硫酸钠(n = 86)进行肠道准备。在基线和肠道准备后第7天和第14天收集粪便样本。采用16S核糖体RNA基因测序分析微生物群的多样性和组成,并进行对比分析。结果:患者肠道菌群的丰度和多样性在肠道准备后显著降低,在第14天没有恢复到肠道准备前的水平。当比较不同的方案时,聚乙二醇和皮硫酸钠基团在丰富度和多样性上分别恢复得更快。结论:在使用不同的肠道准备方案后,肠道微生物群以不同的方式恢复,65岁以上的老年人的微生物结构恢复得更快。
{"title":"Effects of Different Bowel Preparation Regimens and Age Factors on the Gut Microbiota: A Prospective Randomized Controlled Study.","authors":"Yuhang Zhou, Huihui Ji, Shuang Zhang, Xiangrong Zhang, Junhai Zhang, Yujing Wang, Huifen Wang, Yanli Zhang, Shiyu Du","doi":"10.1111/jgh.16868","DOIUrl":"https://doi.org/10.1111/jgh.16868","url":null,"abstract":"<p><strong>Background: </strong>Opinions about the impact of bowel preparation on the gut microbiota are divided. This study investigated the effects of different regimens on the gut microbiota post-bowel preparation and the differences in responses across different age groups.</p><p><strong>Methods: </strong>This single-center, prospective, randomized, controlled clinical trial included 194 patients. Patients were categorized into two groups: one group receiving polyethylene glycol (n = 108) and one receiving sodium picosulfate (n = 86) for bowel preparation. Fecal samples were collected at baseline and on days 7 and 14 post-bowel preparation. The microbiota's diversity and composition were analyzed using 16S ribosomal RNA gene sequencing, followed by comparative analyses.</p><p><strong>Results: </strong>The gut microbiota's abundance and diversity in patients significantly decreased post-bowel preparation, which did not recover to the level of pre-bowel preparation on Day 14. When comparing different regimens, the polyethylene glycol and sodium picosulfate groups recovered faster in richness and diversity, respectively. Patients aged < 65 years had higher richness and diversity of the gut microbiota, whereas the microbiota structure in those aged ≥ 65 years returned to the baseline state faster. The structure of beta diversity is significantly altered and did not return in the short term. However, in the elderly population aged ≥ 65 years, it can rebound quickly. This study also identified a number of significantly altered bacterial genera.</p><p><strong>Conclusions: </strong>Following the use of different bowel preparation regimens, the gut microbiota recovers in diverse ways, with older people over 65 experiencing a faster recovery of the microbial structure.</p>","PeriodicalId":15877,"journal":{"name":"Journal of Gastroenterology and Hepatology","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142950011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Probiotics Combined With Trimebutine for the Treatment of Irritable Bowel Syndrome Patients: A Systematic Review and Meta-Analysis. 益生菌联合曲美布汀治疗肠易激综合征患者:系统回顾和荟萃分析。
IF 3.7 3区 医学 Q2 GASTROENTEROLOGY & HEPATOLOGY Pub Date : 2025-01-08 DOI: 10.1111/jgh.16858
Qiu-Xiang Yu, Dong-Dong Wang, Peng-Ju Dong, Li-Hua Zheng

Objective: This study aimed to assess the efficacy and safety of probiotics combined with trimebutine in the treatment of irritable bowel syndrome (IBS), addressing an important gap in current treatment strategies.

Methods: Randomized controlled trials (RCTs) of trimebutine combined with probiotics for the treatment of IBS were collected from various databases. All retrieved articles were screened and assessed for quality. The methodological quality of the included studies was assessed following the guidelines recommended by the Cochrane Collaboration. The meta-analysis of the included studies was conducted using RevMan 5.3 software.

Results: A total of 37 RCTs involving 4360 participants were included in this study. Among them, the treatment group consisted of 2177 participants, and the control group consisted of 2183 participants. The results showed that the overall efficacy of trimebutine combined with probiotics in the treatment of IBS was significantly higher than that of trimebutine alone (odds ratio [OR] = 5.09, 95 % confidence interval [CI] [4.19, 6.20], p < 0.00001). The effective rate in the combination therapy group was 93.5 % compared with 73.8 % in the trimebutine alone group. The safety profile was favorable, with adverse event rates of 1.75 % and 1.69 % in the combination and monotherapy groups, respectively. The most common adverse events were mild and included dry mouth, nausea and dizziness. No serious adverse events were reported in either group. Subgroup analysis based on the type of probiotic intervention showed that combination use was better than trimebutine alone, and the differences between each subgroup were statistically significant. Combination use of compound Lactobacillus capsules had the best effect (OR = 16.03, 95 % CI [4.57, 56.21], p < 0.0001]. These results highlight the potential role of strain-specific benefits in IBS treatment and suggest that probiotic strain selection may significantly influence treatment outcomes.

Conclusions: The combination of trimebutine and probiotics is more effective in the treatment of IBS compared with trimebutine alone.

Clinical trial registration: https://www.crd.york.ac.uk/prospero/, identifier: CRD42024516044.

目的:本研究旨在评估益生菌联合曲美布汀治疗肠易激综合征(IBS)的有效性和安全性,弥补目前治疗策略的重要空白。方法:从各数据库中收集曲美布汀联合益生菌治疗IBS的随机对照试验(rct)。对所有检索到的文章进行筛选和质量评估。纳入研究的方法学质量按照Cochrane协作网推荐的指南进行评估。采用RevMan 5.3软件对纳入研究进行meta分析。结果:本研究共纳入37项随机对照试验,共纳入4360名受试者。其中,治疗组2177人,对照组2183人。结果显示,曲美布汀联合益生菌治疗IBS的总体疗效显著高于单用曲美布汀(优势比[OR] = 5.09, 95%可信区间[CI] [4.19, 6.20], p)。结论:曲美布汀联合益生菌治疗IBS比单用曲美布汀更有效。临床试验注册:https://www.crd.york.ac.uk/prospero/,标识符:CRD42024516044。
{"title":"Probiotics Combined With Trimebutine for the Treatment of Irritable Bowel Syndrome Patients: A Systematic Review and Meta-Analysis.","authors":"Qiu-Xiang Yu, Dong-Dong Wang, Peng-Ju Dong, Li-Hua Zheng","doi":"10.1111/jgh.16858","DOIUrl":"https://doi.org/10.1111/jgh.16858","url":null,"abstract":"<p><strong>Objective: </strong>This study aimed to assess the efficacy and safety of probiotics combined with trimebutine in the treatment of irritable bowel syndrome (IBS), addressing an important gap in current treatment strategies.</p><p><strong>Methods: </strong>Randomized controlled trials (RCTs) of trimebutine combined with probiotics for the treatment of IBS were collected from various databases. All retrieved articles were screened and assessed for quality. The methodological quality of the included studies was assessed following the guidelines recommended by the Cochrane Collaboration. The meta-analysis of the included studies was conducted using RevMan 5.3 software.</p><p><strong>Results: </strong>A total of 37 RCTs involving 4360 participants were included in this study. Among them, the treatment group consisted of 2177 participants, and the control group consisted of 2183 participants. The results showed that the overall efficacy of trimebutine combined with probiotics in the treatment of IBS was significantly higher than that of trimebutine alone (odds ratio [OR] = 5.09, 95 % confidence interval [CI] [4.19, 6.20], p < 0.00001). The effective rate in the combination therapy group was 93.5 % compared with 73.8 % in the trimebutine alone group. The safety profile was favorable, with adverse event rates of 1.75 % and 1.69 % in the combination and monotherapy groups, respectively. The most common adverse events were mild and included dry mouth, nausea and dizziness. No serious adverse events were reported in either group. Subgroup analysis based on the type of probiotic intervention showed that combination use was better than trimebutine alone, and the differences between each subgroup were statistically significant. Combination use of compound Lactobacillus capsules had the best effect (OR = 16.03, 95 % CI [4.57, 56.21], p < 0.0001]. These results highlight the potential role of strain-specific benefits in IBS treatment and suggest that probiotic strain selection may significantly influence treatment outcomes.</p><p><strong>Conclusions: </strong>The combination of trimebutine and probiotics is more effective in the treatment of IBS compared with trimebutine alone.</p><p><strong>Clinical trial registration: </strong>https://www.crd.york.ac.uk/prospero/, identifier: CRD42024516044.</p>","PeriodicalId":15877,"journal":{"name":"Journal of Gastroenterology and Hepatology","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142950019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
期刊
Journal of Gastroenterology and Hepatology
全部 Acc. Chem. Res. ACS Applied Bio Materials ACS Appl. Electron. Mater. ACS Appl. Energy Mater. ACS Appl. Mater. Interfaces ACS Appl. Nano Mater. ACS Appl. Polym. Mater. ACS BIOMATER-SCI ENG ACS Catal. ACS Cent. Sci. ACS Chem. Biol. ACS Chemical Health & Safety ACS Chem. Neurosci. ACS Comb. Sci. ACS Earth Space Chem. ACS Energy Lett. ACS Infect. Dis. ACS Macro Lett. ACS Mater. Lett. ACS Med. Chem. Lett. ACS Nano ACS Omega ACS Photonics ACS Sens. ACS Sustainable Chem. Eng. ACS Synth. Biol. Anal. Chem. BIOCHEMISTRY-US Bioconjugate Chem. BIOMACROMOLECULES Chem. Res. Toxicol. Chem. Rev. Chem. Mater. CRYST GROWTH DES ENERG FUEL Environ. Sci. Technol. Environ. Sci. Technol. Lett. Eur. J. Inorg. Chem. IND ENG CHEM RES Inorg. Chem. J. Agric. Food. Chem. J. Chem. Eng. Data J. Chem. Educ. J. Chem. Inf. Model. J. Chem. Theory Comput. J. Med. Chem. J. Nat. Prod. J PROTEOME RES J. Am. Chem. Soc. LANGMUIR MACROMOLECULES Mol. Pharmaceutics Nano Lett. Org. Lett. ORG PROCESS RES DEV ORGANOMETALLICS J. Org. Chem. J. Phys. Chem. J. Phys. Chem. A J. Phys. Chem. B J. Phys. Chem. C J. Phys. Chem. Lett. Analyst Anal. Methods Biomater. Sci. Catal. Sci. Technol. Chem. Commun. Chem. Soc. Rev. CHEM EDUC RES PRACT CRYSTENGCOMM Dalton Trans. Energy Environ. Sci. ENVIRON SCI-NANO ENVIRON SCI-PROC IMP ENVIRON SCI-WAT RES Faraday Discuss. Food Funct. Green Chem. Inorg. Chem. Front. Integr. Biol. J. Anal. At. Spectrom. J. Mater. Chem. A J. Mater. Chem. B J. Mater. Chem. C Lab Chip Mater. Chem. Front. Mater. Horiz. MEDCHEMCOMM Metallomics Mol. Biosyst. Mol. Syst. Des. Eng. Nanoscale Nanoscale Horiz. Nat. Prod. Rep. New J. Chem. Org. Biomol. Chem. Org. Chem. Front. PHOTOCH PHOTOBIO SCI PCCP Polym. Chem.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1