Pub Date : 2025-12-01Epub Date: 2025-10-06DOI: 10.1016/j.jgar.2025.09.015
Hui Wang , Jie Sheng , Ying Zhang , Hao Lan , Xiaofeng Lu , Xi Li , Xiaofei Zhao
Objective
Q (query) fever, caused by Coxiella burnetii, is often linked to negative bacterial cultures, with infective endocarditis with negative cultures difficult to diagnose and treat. Our case demonstrates that metagenomic next-generation sequencing (mNGS) is able to provide a rapid and accurate method for pathogenetic diagnosis in infectious diseases.
Case presentation
A case of infective endocarditis with negative blood cultures is reported in a male patient with a history of sheep farming and previous aortic valve replacement and atrial septal defect atrial septal defect repair. Blood tests showed positive serum immunofluorescent antibodies to Rickettsia, while mNGS of perivalvular abscess tissue suggested C. burnetii. Doxycycline 0.1 g q12h and hydroxychloroquine 0.2 g q12h were used for postoperative antibiotic treatment. The genome of C. burnetii C2245173Z was assembled on the Illumina platform and no known antibiotic resistance genes were detected. Phylogenetic analysis of the C. burnetii genome showed a genetic relationship between animal- and human-derived strains.
Conclusions
mNGS could provide a rapid and accurate assay for clinical diagnosis and play a decisive role in the pathogenetic diagnosis of some infectious diseases. Doxycycline plus hydroxychloroquine remains an effective treatment for chronic Q fever endocarditis. In addition, phylogenetic tree analysis indicates that C. burnetii infection may pose a potential risk to humans working with livestock.
目的：由伯纳氏克希菌引起的发热通常与阴性细菌培养有关。阴性培养的感染性心内膜炎很难诊断和治疗。我们的案例表明，新一代宏基因组测序（mNGS）可以为传染病的病理诊断提供快速准确的方法。病例介绍：我们报告了一例感染性心内膜炎伴阴性血培养的男性患者，有养羊史，有主动脉瓣置换术（AVT）和房间隔缺损（ASD）修复史。血液检查显示血清立克次体免疫荧光抗体阳性，而瓣膜周围脓肿组织的mNGS提示伯氏原体感染。术后应用强力霉素0.1 g q12h、羟氯喹0.2 g q12h给予抗生素治疗。在Illumina平台上组装burnetii C2245173Z基因组，未检测到已知的抗生素耐药基因。伯纳蒂胞杆菌基因组的系统发育分析显示动物源性菌株和人源性菌株之间存在遗传关系。结论：应用mNGS可为临床诊断提供快速、准确的检测方法，在某些感染性疾病的发病诊断中具有决定性作用。强力霉素加羟氯喹仍然是治疗慢性Q热心内膜炎的有效方法。此外，系统发育树分析表明，伯纳蒂胞杆菌感染可能对与牲畜一起工作的人构成潜在风险。
{"title":"Detection of zoonotic Coxiella burnetii causing chronic Q fever endocarditis in a Chinese geriatric patient by mNGS","authors":"Hui Wang , Jie Sheng , Ying Zhang , Hao Lan , Xiaofeng Lu , Xi Li , Xiaofei Zhao","doi":"10.1016/j.jgar.2025.09.015","DOIUrl":"10.1016/j.jgar.2025.09.015","url":null,"abstract":"<div><h3>Objective</h3><div>Q (query) fever, caused by <em>Coxiella burnetii</em>, is often linked to negative bacterial cultures, with infective endocarditis with negative cultures difficult to diagnose and treat. Our case demonstrates that metagenomic next-generation sequencing (mNGS) is able to provide a rapid and accurate method for pathogenetic diagnosis in infectious diseases.</div></div><div><h3>Case presentation</h3><div>A case of infective endocarditis with negative blood cultures is reported in a male patient with a history of sheep farming and previous aortic valve replacement and atrial septal defect atrial septal defect repair. Blood tests showed positive serum immunofluorescent antibodies to <em>Rickettsia</em>, while mNGS of perivalvular abscess tissue suggested <em>C. burnetii</em>. Doxycycline 0.1 g q12h and hydroxychloroquine 0.2 g q12h were used for postoperative antibiotic treatment. The genome of <em>C. burnetii</em> C2245173Z was assembled on the Illumina platform and no known antibiotic resistance genes were detected. Phylogenetic analysis of the <em>C. burnetii</em> genome showed a genetic relationship between animal- and human-derived strains.</div></div><div><h3>Conclusions</h3><div>mNGS could provide a rapid and accurate assay for clinical diagnosis and play a decisive role in the pathogenetic diagnosis of some infectious diseases. Doxycycline plus hydroxychloroquine remains an effective treatment for chronic Q fever endocarditis. In addition, phylogenetic tree analysis indicates that <em>C. burnetii</em> infection may pose a potential risk to humans working with livestock.</div></div>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"45 ","pages":"Pages 192-195"},"PeriodicalIF":3.2,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145251360","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-12-01Epub Date: 2025-09-19DOI: 10.1016/j.jgar.2025.09.006
Lin Zheng , Yanwan Shangguan , Wanru Guo , Zhongkang Ji , Kaijin Xu
In recent years, the detection rate of nontuberculous mycobacteria has increased, drawing attention in clinical settings. Here, we present a case of pulmonary Pseudomonas aeruginosa and Mycobacterium wolinskyi coinfection in a 60-year-old hemodialysis recipient. To the best of our knowledge, this is the first instance of isolating the M. wolinskyi strain from human respiratory samples, including a comprehensive exploration of its susceptibility to clinically available antimicrobials. This discovery deepened our understanding of the infection spectrum of M. wolinskyi, highlighting the need to consider the potential for coinfections with common pathogens and less common atypical pathogens when managing immunocompromised patients. Furthermore, this study also assessed the in vitro antibacterial activity of newly available antibacterial drugs in clinical practice against the M. wolinskyi strain.
{"title":"Uncommon pulmonary mycobacterium wolinskyi infection in a hemodialysis patient","authors":"Lin Zheng , Yanwan Shangguan , Wanru Guo , Zhongkang Ji , Kaijin Xu","doi":"10.1016/j.jgar.2025.09.006","DOIUrl":"10.1016/j.jgar.2025.09.006","url":null,"abstract":"<div><div>In recent years, the detection rate of nontuberculous mycobacteria has increased, drawing attention in clinical settings. Here, we present a case of pulmonary <em>Pseudomonas aeruginosa</em> and <em>Mycobacterium wolinskyi</em> coinfection in a 60-year-old hemodialysis recipient. To the best of our knowledge, this is the first instance of isolating the <em>M. wolinskyi</em> strain from human respiratory samples, including a comprehensive exploration of its susceptibility to clinically available antimicrobials. This discovery deepened our understanding of the infection spectrum of <em>M. wolinskyi</em>, highlighting the need to consider the potential for coinfections with common pathogens and less common atypical pathogens when managing immunocompromised patients. Furthermore, this study also assessed the in vitro antibacterial activity of newly available antibacterial drugs in clinical practice against the <em>M. wolinskyi</em> strain.</div></div>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"45 ","pages":"Pages 134-137"},"PeriodicalIF":3.2,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145102878","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-12-01Epub Date: 2025-09-24DOI: 10.1016/j.jgar.2025.09.009
Nada A. Alsaleh , Abeer AlSmari , Abrar F. Alhameed , Ahmed O. Alenazi , Alaa A. Alsharif , Amal Bin Akresh , Anwar M. Alnakhli , Bashaier Alshehail , Eman A. Alzahrani , Ghadah H. Alshehri , Ghazwa B. Korayem , Hanan A. Bakri , Khalid Eljaaly , Lina I. Alnajjar , Norah S. Aldeghaither , Reem Almahasna , Sara Almuhisen , Yassmin Alsomali , Zikria Saleem
Background
Antimicrobial resistance (AMR) threatens global health by reducing the efficacy of common infection treatments. This study examines antimicrobial use in Saudi Arabian hospitals, identifies influencing factors, and proposes interventions using the World Health Organization’s (WHO) Access, Watch, Reserve (AWaRe) classification system.
Methods
A cross-sectional, multicentre point prevalence survey (PPS) of antimicrobial utilization was conducted in 10 hospitals across 6 regions of Saudi Arabia September 2023. All inpatients receiving antimicrobials on the PPS day were included. Data collection utilized the Global PPS tool developed by the University of Antwerp, Belgium.
Results
Among 2890 inpatients, 766 (26.5%) were prescribed at least one antimicrobial, resulting in a total of 982 prescriptions. The primary indications for these antimicrobials were community-acquired infections (37.1%), Healthcare-associated infections (35.9%), surgical prophylaxis (15.4%), unknown reasons (8.7%), medical prophylaxis (2.5%), and other reasons (0.3%). The most common reasons for antimicrobial use included pneumonia or lower respiratory tract infections (16.1%), skin and soft tissue infections (11%) and bacteraemia (8.9%). The most frequently prescribed antimicrobial classes were penicillins with beta-lactamase inhibitors (18.5%), carbapenems (15.7%), and third-generation cephalosporins (11.1%). Most of the antimicrobials (66.3%) were classified as Watch antimicrobials, followed by 23.8% as Access, and 8.9% as Reserve.
Conclusions
The study provides valuable insights into antimicrobial utilization in Saudi Arabia, offering a baseline for assessing prescribing patterns. While findings may reflect certain antimicrobial stewardship efforts, further investigation is needed to evaluate their impact. The study also highlights key areas for improvement, emphasizing the importance of conducting future PPS to guide antimicrobial stewardship strategies and monitor progress in managing AMR.
{"title":"Antimicrobial utilization among hospitalized patients according to WHO AWaRe classification: Results from a multicentre point prevalence survey in Saudi Arabia","authors":"Nada A. Alsaleh , Abeer AlSmari , Abrar F. Alhameed , Ahmed O. Alenazi , Alaa A. Alsharif , Amal Bin Akresh , Anwar M. Alnakhli , Bashaier Alshehail , Eman A. Alzahrani , Ghadah H. Alshehri , Ghazwa B. Korayem , Hanan A. Bakri , Khalid Eljaaly , Lina I. Alnajjar , Norah S. Aldeghaither , Reem Almahasna , Sara Almuhisen , Yassmin Alsomali , Zikria Saleem","doi":"10.1016/j.jgar.2025.09.009","DOIUrl":"10.1016/j.jgar.2025.09.009","url":null,"abstract":"<div><h3>Background</h3><div>Antimicrobial resistance (AMR) threatens global health by reducing the efficacy of common infection treatments. This study examines antimicrobial use in Saudi Arabian hospitals, identifies influencing factors, and proposes interventions using the World Health Organization’s (WHO) Access, Watch, Reserve (AWaRe) classification system.</div></div><div><h3>Methods</h3><div>A cross-sectional, multicentre point prevalence survey (PPS) of antimicrobial utilization was conducted in 10 hospitals across 6 regions of Saudi Arabia September 2023. All inpatients receiving antimicrobials on the PPS day were included. Data collection utilized the Global PPS tool developed by the University of Antwerp, Belgium.</div></div><div><h3>Results</h3><div>Among 2890 inpatients, 766 (26.5%) were prescribed at least one antimicrobial, resulting in a total of 982 prescriptions. The primary indications for these antimicrobials were community-acquired infections (37.1%), Healthcare-associated infections (35.9%), surgical prophylaxis (15.4%), unknown reasons (8.7%), medical prophylaxis (2.5%), and other reasons (0.3%). The most common reasons for antimicrobial use included pneumonia or lower respiratory tract infections (16.1%), skin and soft tissue infections (11%) and bacteraemia (8.9%). The most frequently prescribed antimicrobial classes were penicillins with beta-lactamase inhibitors (18.5%), carbapenems (15.7%), and third-generation cephalosporins (11.1%). Most of the antimicrobials (66.3%) were classified as Watch antimicrobials, followed by 23.8% as Access, and 8.9% as Reserve.</div></div><div><h3>Conclusions</h3><div>The study provides valuable insights into antimicrobial utilization in Saudi Arabia, offering a baseline for assessing prescribing patterns. While findings may reflect certain antimicrobial stewardship efforts, further investigation is needed to evaluate their impact. The study also highlights key areas for improvement, emphasizing the importance of conducting future PPS to guide antimicrobial stewardship strategies and monitor progress in managing AMR.</div></div>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"45 ","pages":"Pages 147-156"},"PeriodicalIF":3.2,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145176153","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-12-01Epub Date: 2025-10-10DOI: 10.1016/j.jgar.2025.09.007
Ling Xin, Jing Wang, Cong Qin, Yi Sun, Hongwei Zhou, Rong Zhang, Gongxiang Chen
Objective
A highly virulent Klebsiella pneumoniae ST23-K1 carrying blaKPC-3, named as Kpn_24-5, was identified in the blood samples of a patient with multiple complications. We investigated the transfer and pathogenic mechanism of this strain, and elucidated the epidemiological situation of the strain.
Methods
A blaKPC-3-positive ST23-K1 K. pneumoniae was isolated. Antimicrobial susceptibility test and whole genome sequencing were used to study the phenotypes and genotypes of the strain. The transmission and pathogenic mechanism were investigated by means of conjugation transfer test, neutrophil killing test, and serum inhibition test.
Results
The Kpn_24-5 strain was ST23 sequence type and K64:O1 capsular serotype, which contains four plasmids. Plasmid 1 carries several virulence genes, belonging to the same clade as K. pneumoniae plasmid isolated from the USA in 2023. Plasmid 2 is an anti-plasmid containing blaKPC-3 carbapenem resistance gene, belonging to the same clade as K. pneumoniae plasmid isolated from China in 2024. The genetic characteristics of plasmids indicated that the genetic environment of blaKPC-3 in plasmid 2 of Kpn_24-5 strain was IS26-ISKpn27-blaKPC-3-Tn21. The resistance plasmid 2 of Kpn_24-5 strain was transferable. The Kpn_24-5 strain has higher tolerance to neutrophils, is not easily inhibited by serum, and has significantly higher infectivity.
Conclusions
The present study corroborates the emergence of hypervirulent CR-hvKP in the Zhejiang region, a phenomenon that is presumably attributable to the acquisition of an IncFIB virulence plasmid and a blaKPC-3 resistance plasmid by the ST23-K1 type K. pneumoniae. Our findings highlight the urgent need to use antibiotics more wisely to limit the emergence of resistance.
{"title":"Emergence of ST23-K1 blaKPC-3-positive hypervirulent carbapenem-resistant Klebsiella pneumoniae from China: A molecular, biological, and epidemiological study","authors":"Ling Xin, Jing Wang, Cong Qin, Yi Sun, Hongwei Zhou, Rong Zhang, Gongxiang Chen","doi":"10.1016/j.jgar.2025.09.007","DOIUrl":"10.1016/j.jgar.2025.09.007","url":null,"abstract":"<div><h3>Objective</h3><div>A highly virulent <em>Klebsiella pneumoniae</em> ST23-K1 carrying <em>bla</em><sub>KPC-3</sub>, named as Kpn_24-5, was identified in the blood samples of a patient with multiple complications. We investigated the transfer and pathogenic mechanism of this strain, and elucidated the epidemiological situation of the strain.</div></div><div><h3>Methods</h3><div>A <em>bla</em><sub>KPC-3-</sub>positive ST23-K1 <em>K. pneumoniae</em> was isolated. Antimicrobial susceptibility test and whole genome sequencing were used to study the phenotypes and genotypes of the strain. The transmission and pathogenic mechanism were investigated by means of conjugation transfer test, neutrophil killing test, and serum inhibition test.</div></div><div><h3>Results</h3><div>The Kpn_24-5 strain was ST23 sequence type and K64:O1 capsular serotype, which contains four plasmids. Plasmid 1 carries several virulence genes, belonging to the same clade as <em>K. pneumoniae</em> plasmid isolated from the USA in 2023. Plasmid 2 is an anti-plasmid containing <em>bla</em><sub>KPC-3</sub> carbapenem resistance gene, belonging to the same clade as <em>K. pneumoniae</em> plasmid isolated from China in 2024. The genetic characteristics of plasmids indicated that the genetic environment of <em>bla</em><sub>KPC-3</sub> in plasmid 2 of Kpn_24-5 strain was IS26-ISKpn27-<em>bla</em><sub>KPC-3</sub>-Tn21. The resistance plasmid 2 of Kpn_24-5 strain was transferable. The Kpn_24-5 strain has higher tolerance to neutrophils, is not easily inhibited by serum, and has significantly higher infectivity.</div></div><div><h3>Conclusions</h3><div>The present study corroborates the emergence of hypervirulent CR-hvKP in the Zhejiang region, a phenomenon that is presumably attributable to the acquisition of an IncFIB virulence plasmid and a <em>bla</em><sub>KPC-3</sub> resistance plasmid by the ST23-K1 type <em>K. pneumoniae</em>. Our findings highlight the urgent need to use antibiotics more wisely to limit the emergence of resistance.</div></div>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"45 ","pages":"Pages 234-239"},"PeriodicalIF":3.2,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145280415","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The Mississippi Public Health Laboratory conducts active surveillance of carbapenem-resistant Enterobacterales (CRE) infections as part of the Antimicrobial Resistance Laboratory Network (ARLN) program implemented by the United States Centers for Disease Control and Prevention (CDC). Here, we describe the prevalence of New Delhi Metallo-β-lactamase (blaNDM) genes in Klebsiella, a major pathogen of the Enterobacterales order linked to nosocomial infections, between January 2022 and June 2025.
Methods
Carbapenemase activity was confirmed using the Modified Carbapenem Inactivation Method (mCIM). PCR was then performed on carbapenemase-positive isolates to detect blaNDM genes. Whole-genome sequencing (WGS) was performed on 35 blaNDM-positive isolates to identify blaNDM gene variants, other antimicrobial resistance (AMR) determinants, virulence factors, and plasmids. Antibiotic susceptibility testing (AST) was determined by the Kirby-Bauer disk diffusion assay.
Results
Among the 35 NDM-positive Klebsiella species, the blaNDM-5 variant was identified in 23 (65.7%) isolates, followed by blaNDM-1 and blaNDM-7 genes which were identified in 6/35 (17%) and 6/35 (17%) cases, respectively. A significant proportion of blaNDM-5 cases also harbored additional β-lactamase genes such as blaSHV family (18/23; 78%) and blaTEM-1 (9/23; 39%) indicating multi-drug resistance. All 23 blaNDM-5-positive isolates were resistant to carbapenems and broad-spectrum cephalosporins, but 13/23 (56.5%) remained susceptible to aztreonam. PlasmidFinder analysis identified IncX3 (20/23) and IncFIB(K) (19/23) as the predominant plasmid replicons among these isolates.
Conclusion
The emergence and spread of bacteria carrying blaNDM-5 gene poses a significant threat to public health in Mississippi. Our dataset warrants further study to investigate the potential role of plasmids in blaNDM-5 dissemination.
{"title":"blaNDM-5 is the predominant variant of blaNDM gene in Klebsiella species detected in Mississippi, USA","authors":"Srimadhav Nallani , Saihou Ceesay , Abiye Iyo, Lucersia Nichols, Daphne Ware, Beata Karolewicz","doi":"10.1016/j.jgar.2025.10.014","DOIUrl":"10.1016/j.jgar.2025.10.014","url":null,"abstract":"<div><h3>Objectives</h3><div>The Mississippi Public Health Laboratory conducts active surveillance of carbapenem-resistant <em>Enterobacterales</em> (CRE) infections as part of the Antimicrobial Resistance Laboratory Network (ARLN) program implemented by the United States Centers for Disease Control and Prevention (CDC). Here, we describe the prevalence of New Delhi Metallo-β-lactamase (<em>bla</em><sub>NDM</sub>) genes in <em>Klebsiella</em>, a major pathogen of the <em>Enterobacterales</em> order linked to nosocomial infections, between January 2022 and June 2025.</div></div><div><h3>Methods</h3><div>Carbapenemase activity was confirmed using the Modified Carbapenem Inactivation Method (mCIM). PCR was then performed on carbapenemase-positive isolates to detect <em>bla</em><sub>NDM</sub> genes. Whole-genome sequencing (WGS) was performed on 35 <em>bla</em><sub>NDM</sub>-positive isolates to identify <em>bla</em><sub>NDM</sub> gene variants, other antimicrobial resistance (AMR) determinants, virulence factors, and plasmids. Antibiotic susceptibility testing (AST) was determined by the Kirby-Bauer disk diffusion assay.</div></div><div><h3>Results</h3><div>Among the 35 NDM-positive <em>Klebsiella</em> species, the <em>bla</em><sub>NDM-5</sub> variant was identified in 23 (65.7%) isolates, followed by <em>bla</em><sub>NDM-1</sub> and <em>bla</em><sub>NDM-7</sub> genes which were identified in 6/35 (17%) and 6/35 (17%) cases, respectively. A significant proportion of <em>bla</em><sub>NDM-5</sub> cases also harbored additional β-lactamase genes such as <em>bla</em><sub>SHV</sub> family (18/23; 78%) and <em>bla</em><sub>TEM-1</sub> (9/23; 39%) indicating multi-drug resistance. All 23 <em>bla</em><sub>NDM-5</sub>-positive isolates were resistant to carbapenems and broad-spectrum cephalosporins, but 13/23 (56.5%) remained susceptible to aztreonam. PlasmidFinder analysis identified IncX3 (20/23) and IncFIB(K) (19/23) as the predominant plasmid replicons among these isolates.</div></div><div><h3>Conclusion</h3><div>The emergence and spread of bacteria carrying <em>bla</em><sub>NDM-5</sub> gene poses a significant threat to public health in Mississippi. Our dataset warrants further study to investigate the potential role of plasmids in <em>bla</em><sub>NDM-5</sub> dissemination.</div></div>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"45 ","pages":"Pages 282-286"},"PeriodicalIF":3.2,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145354938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-12-01Epub Date: 2025-10-27DOI: 10.1016/j.jgar.2025.10.023
Xiaoqin Niu , Liping Tu , Qinlong Yu , Binbin Lu , Junhua Tian , Wei Wang , Bailong Hou , Weifeng Shen
Background
Salmonella is a worldwide food-borne pathogen, which brings significant challenges to clinical practice due to the increase of antibiotic resistance.
Objective
This study aims to explore the serotype variety and antibiotic resistance of food-borne Salmonella in the Jiaxing region from 2020 to 2024.
Methods
The faecal samples of 8541 patients with food-borne diseases were investigated. The isolated Salmonella strains underwent MALDI-TOF, serological typing, and drug susceptibility testing.
Results
A total of 622 Salmonella strains were isolated from 8541 faecal samples, with 382 (61.4%) originating from patients under 6 y old. Summer and autumn were the peak seasons. 622 strains of Salmonella were divided into 53 serotypes, with the most common serotypes being Salmonella Typhimurium and Salmonella Enteritidis. The drug susceptibility test revealed that most strains were resistant to ampicillin (79.8%), while 38% exhibited resistance to trimethoprim/sulfamethoxazole. The susceptibility rate to cephalosporins and fluoroquinolones was over 84%. Only one strain was found to be resistant to carbapenems. Notably, 69.5% of the strains were resistant to at least one antibiotic, and 21.9% demonstrated multidrug resistance. Our findings also indicated an increasing trend in the resistance of isolated strains to ceftriaxone and cefepime over the past 5 y. Compared to S. Typhimurium, S. Enteritidis was more susceptible to a range of antibiotics, with the most notable difference in susceptibility to trimethoprim/sulfamethoxazole.
Conclusions
Salmonella serotypes exhibited varied patterns of drug susceptibility. Antibiotic selection should be based on the susceptibility profile of the pathogenic bacteria to effectively treat and prevent Salmonella infections.
{"title":"Serotypes and antimicrobial resistance patterns of food-borne Salmonella isolates in the Jiaxing Region, China, from 2020 to 2024","authors":"Xiaoqin Niu , Liping Tu , Qinlong Yu , Binbin Lu , Junhua Tian , Wei Wang , Bailong Hou , Weifeng Shen","doi":"10.1016/j.jgar.2025.10.023","DOIUrl":"10.1016/j.jgar.2025.10.023","url":null,"abstract":"<div><h3>Background</h3><div><em>Salmonella</em> is a worldwide food-borne pathogen, which brings significant challenges to clinical practice due to the increase of antibiotic resistance.</div></div><div><h3>Objective</h3><div>This study aims to explore the serotype variety and antibiotic resistance of food-borne <em>Salmonella</em> in the Jiaxing region from 2020 to 2024.</div></div><div><h3>Methods</h3><div>The faecal samples of 8541 patients with food-borne diseases were investigated. The isolated <em>Salmonella</em> strains underwent MALDI-TOF, serological typing, and drug susceptibility testing.</div></div><div><h3>Results</h3><div>A total of 622 <em>Salmonella</em> strains were isolated from 8541 faecal samples, with 382 (61.4%) originating from patients under 6 y old. Summer and autumn were the peak seasons. 622 strains of <em>Salmonella</em> were divided into 53 serotypes, with the most common serotypes being <em>Salmonella</em> Typhimurium and <em>Salmonella</em> Enteritidis. The drug susceptibility test revealed that most strains were resistant to ampicillin (79.8%), while 38% exhibited resistance to trimethoprim/sulfamethoxazole. The susceptibility rate to cephalosporins and fluoroquinolones was over 84%. Only one strain was found to be resistant to carbapenems. Notably, 69.5% of the strains were resistant to at least one antibiotic, and 21.9% demonstrated multidrug resistance. Our findings also indicated an increasing trend in the resistance of isolated strains to ceftriaxone and cefepime over the past 5 y. Compared to <em>S</em>. Typhimurium, <em>S</em>. Enteritidis was more susceptible to a range of antibiotics, with the most notable difference in susceptibility to trimethoprim/sulfamethoxazole.</div></div><div><h3>Conclusions</h3><div><em>Salmonella</em> serotypes exhibited varied patterns of drug susceptibility. Antibiotic selection should be based on the susceptibility profile of the pathogenic bacteria to effectively treat and prevent <em>Salmonella</em> infections.</div></div>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"45 ","pages":"Pages 294-298"},"PeriodicalIF":3.2,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145401066","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-12-01Epub Date: 2025-08-25DOI: 10.1016/j.jgar.2025.08.011
Mohamed Mediouni, Vladimir Makarenkov, Abdoulaye Baniré Diallo
This article explores the main stages of developing an interpretable machine learning (ML) model for predicting antimicrobial resistance (AMR), highlighting the importance of model interpretability in enhancing the prediction performance. By integrating phenotype-genotype synergy, our goal is to better understand AMR mechanisms. Such an approach combines ML with biological insights, offering a pathway towards more reliable AMR predictions and advancing the discovery of effective treatments against resistant pathogens. The challenges and opportunities related to incorporating this synergy into an ML model are discussed.
{"title":"Towards an interpretable machine learning model for predicting antimicrobial resistance","authors":"Mohamed Mediouni, Vladimir Makarenkov, Abdoulaye Baniré Diallo","doi":"10.1016/j.jgar.2025.08.011","DOIUrl":"10.1016/j.jgar.2025.08.011","url":null,"abstract":"<div><div>This article explores the main stages of developing an interpretable machine learning (ML) model for predicting antimicrobial resistance (AMR), highlighting the importance of model interpretability in enhancing the prediction performance. By integrating phenotype-genotype synergy, our goal is to better understand AMR mechanisms. Such an approach combines ML with biological insights, offering a pathway towards more reliable AMR predictions and advancing the discovery of effective treatments against resistant pathogens. The challenges and opportunities related to incorporating this synergy into an ML model are discussed.</div></div>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"45 ","pages":"Pages 47-51"},"PeriodicalIF":3.2,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144956924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-12-01Epub Date: 2025-07-14DOI: 10.1016/j.jgar.2025.07.008
Onyansaniba K. Ntim , Aaron Awere-Duodu , Samuel Addo Akwetey , Fleischer C.N. Kotey , Eric S. Donkor
Background
The diversity of pneumococcal serotypes poses significant challenges for both vaccination and treatment. Clinical management of pneumococcal infections is further complicated by the fact that individual serotypes exhibit distinct antimicrobial resistance patterns. This study aims to comprehensively estimate the antimicrobial resistance rates of pneumococcal serotypes.
Methods
A comprehensive search was conducted in PubMed, Scopus, Web of Science, and ScienceDirect. A random-effect meta-analysis was used to pool the number of isolates resistant to a particular antimicrobial per total number of isolates tested for each pneumococcal serotype.
Results
Ninety studies were included in the analysis. The study identified 66 pneumococcal serotypes, with vaccine serotypes such as 19A, 19F, 23F, 6B, 14, 6A, 3, and 9V being the most frequently reported. Non-vaccine serotypes like 15A, 6C, 23A, 23B, 15B, and 35B were also prevalent. Most serotypes were associated with pneumococcal diseases. Serotypes exhibited varying and high resistance to cefuroxime, co-trimoxazole, tetracycline, macrolides, and clindamycin. The highest multidrug resistance was observed in serotypes 23F (63.34%, 95% CI [25.77; 94.31]), 15C (61.04%, 95% CI [0.74; 100.00]), 23A (57.28%, 95% CI [27.60; 84.58]), 19F (55.95%, 95% CI [30.26; 80.22]), 19A (54.07%, 95% CI [32.60; 74.90]), 15B (47.10%, 95% CI [9.66; 86.41]), 24F (45.77%, 95% CI [3.79; 91.31]), 15A (44.28%, 95% CI [29.58; 59.26]), and 6B (43.79%, 95% CI [28.48; 59.12]).
Conclusions
This study highlights significant antimicrobial resistance among both vaccine (19A, 19F, 23F, 14, 6A, and 6B) and non-vaccine types (15A, 6C, 23A, 20, 15C, and 35B). Our findings emphasize the need for effective surveillance and targeted interventions to fight these resistant pneumococcal serotypes.
背景：肺炎球菌的多种血清型使疫苗接种和治疗具有挑战性。治疗并发症是不同血清型表现出不同耐药率的结果。本研究旨在综合估计肺炎球菌血清型的抗菌素耐药率。方法：综合检索PubMed、Scopus、Web of Science、ScienceDirect。随机效应荟萃分析用于汇总每种肺炎球菌血清型检测的分离株总数中对特定抗菌素耐药的分离株数量。结果：90项研究被纳入分析。该研究确定了66种肺炎球菌血清型，其中最常报告的疫苗血清型包括19A、19F、23F、6B、14、6A、3和9V。非疫苗血清型如15A、6C、23A、23B、15B和35B也很普遍。大多数血清型与肺炎球菌疾病相关。血清型对头孢呋辛、复方新诺明、四环素、大环内酯类和克林霉素表现出不同程度的高耐药性。血清型23F为多药耐药最高(63.34%,95% CI [25.77；94.31]), 15c (61.04%, 95% ci [0.74；100.00]), 23a (57.28%, 95% ci [27.60；[84.58]), 19f (55.95%, 95% ci [30.26；80.22]), 19a (54.07%, 95% ci [32.60；74.90]), 15b (47.10%, 95% ci [9.66；86.41]), 24f (45.77%, 95% ci [3.79；91.31]), 15a (44.28%, 95% ci [29.58；59.26]), 6B (43.79%, 95% CI [28.48；59.12])。结论：本研究强调疫苗（19A、19F、23F、14、6A和6B）和非疫苗类型（15A、6C、23A、20、15C和35B）均存在显著的抗微生物药物耐药性。我们的研究结果强调需要有效的监测和有针对性的干预措施来对抗这些耐药肺炎球菌血清型。
{"title":"Relationship between pneumococcal serotypes and antimicrobial resistance: A systematic review and meta-analysis","authors":"Onyansaniba K. Ntim , Aaron Awere-Duodu , Samuel Addo Akwetey , Fleischer C.N. Kotey , Eric S. Donkor","doi":"10.1016/j.jgar.2025.07.008","DOIUrl":"10.1016/j.jgar.2025.07.008","url":null,"abstract":"<div><h3>Background</h3><div>The diversity of pneumococcal serotypes poses significant challenges for both vaccination and treatment. Clinical management of pneumococcal infections is further complicated by the fact that individual serotypes exhibit distinct antimicrobial resistance patterns. This study aims to comprehensively estimate the antimicrobial resistance rates of pneumococcal serotypes.</div></div><div><h3>Methods</h3><div>A comprehensive search was conducted in PubMed, Scopus, Web of Science, and ScienceDirect. A random-effect meta-analysis was used to pool the number of isolates resistant to a particular antimicrobial per total number of isolates tested for each pneumococcal serotype.</div></div><div><h3>Results</h3><div>Ninety studies were included in the analysis. The study identified 66 pneumococcal serotypes, with vaccine serotypes such as 19A, 19F, 23F, 6B, 14, 6A, 3, and 9V being the most frequently reported. Non-vaccine serotypes like 15A, 6C, 23A, 23B, 15B, and 35B were also prevalent. Most serotypes were associated with pneumococcal diseases. Serotypes exhibited varying and high resistance to cefuroxime, co-trimoxazole, tetracycline, macrolides, and clindamycin. The highest multidrug resistance was observed in serotypes 23F (63.34%, 95% CI [25.77; 94.31]), 15C (61.04%, 95% CI [0.74; 100.00]), 23A (57.28%, 95% CI [27.60; 84.58]), 19F (55.95%, 95% CI [30.26; 80.22]), 19A (54.07%, 95% CI [32.60; 74.90]), 15B (47.10%, 95% CI [9.66; 86.41]), 24F (45.77%, 95% CI [3.79; 91.31]), 15A (44.28%, 95% CI [29.58; 59.26]), and 6B (43.79%, 95% CI [28.48; 59.12]).</div></div><div><h3>Conclusions</h3><div>This study highlights significant antimicrobial resistance among both vaccine (19A, 19F, 23F, 14, 6A, and 6B) and non-vaccine types (15A, 6C, 23A, 20, 15C, and 35B). Our findings emphasize the need for effective surveillance and targeted interventions to fight these resistant pneumococcal serotypes.</div></div>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"45 ","pages":"Pages 52-67"},"PeriodicalIF":3.2,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144649661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-12-01Epub Date: 2025-08-13DOI: 10.1016/j.jgar.2025.08.004
Lu Liu , Shuang Wang , Yanru Chen , Xinyu Xian , Xiaolin Yu , Yan Li , Yuzhen Chen , Gaoxiang Sun , Lixiao Cheng , Ti Liu , Zengqiang Kou
Objectives
This study investigated the prevalence of nasal carriage, molecular characteristics, antimicrobial resistance (AMR) patterns, and phylogenetic relationships of Staphylococcus aureus among healthy individuals in rural communities in China.
Methods
Nasal swabs were obtained from 646 asymptomatic residents across 10 villages. All S. aureus isolates underwent antimicrobial susceptibility testing and whole-genome sequencing. Comprehensive genomic analyses were performed to predict antibiotic resistance genes and to determine multilocus sequence typing (MLST), spa types, and Staphylococcal Cassette Chromosome mec (SCCmec) classifications, along with other molecular features. Phylogenetic relationships were inferred using single-nucleotide polymorphism (SNP)-based analysis.
Results
From 646 nasal swabs collected from healthy individuals in rural communities, 48 S. aureus isolates were recovered. Among these, 16.67% (8/48) isolates were identified as methicillin-resistant S. aureus (MRSA), resulting in an overall MRSA carriage rate of 1.24% (8/646); 75% (36/48) of the isolates were identified as multidrug-resistant, and the resistance rates to penicillin and erythromycin were relatively high, at 95.83% (46/48) and 91.67% (44/48) respectively. In total, 23 spa types and 12 MLSTs were identified, with t34, ST398, and ST59 being the most prevalent. Dominant MRSA clones included ST59-t437-IVa and ST9-t899-XII. Phylogenetic analysis revealed close genetic relatedness between local isolates and strains of porcine origin or those previously reported in Germany, indicating potential interspecies and transboundary transmission pathways.
Conclusion
The findings highlighted a concerning S. aureus AMR burden among healthy rural populations in China and provided molecular evidence supporting the existence of animal-environment-human transmission routes. These results underscored critical gaps in current One Health frameworks and called for urgent, integrated interventions to mitigate the complex and interconnected nature of AMR threats.
{"title":"Nasal carriage of staphylococcus aureus in rural China: Molecular epidemiology and antimicrobial susceptibility","authors":"Lu Liu , Shuang Wang , Yanru Chen , Xinyu Xian , Xiaolin Yu , Yan Li , Yuzhen Chen , Gaoxiang Sun , Lixiao Cheng , Ti Liu , Zengqiang Kou","doi":"10.1016/j.jgar.2025.08.004","DOIUrl":"10.1016/j.jgar.2025.08.004","url":null,"abstract":"<div><h3>Objectives</h3><div>This study investigated the prevalence of nasal carriage, molecular characteristics, antimicrobial resistance (AMR) patterns, and phylogenetic relationships of <em>Staphylococcus aureus</em> among healthy individuals in rural communities in China.</div></div><div><h3>Methods</h3><div>Nasal swabs were obtained from 646 asymptomatic residents across 10 villages. All <em>S. aureus</em> isolates underwent antimicrobial susceptibility testing and whole-genome sequencing. Comprehensive genomic analyses were performed to predict antibiotic resistance genes and to determine multilocus sequence typing (MLST), <em>spa</em> types, and <em>Staphylococcal Cassette Chromosome mec</em> (SCCmec) classifications, along with other molecular features. Phylogenetic relationships were inferred using single-nucleotide polymorphism (SNP)-based analysis.</div></div><div><h3>Results</h3><div>From 646 nasal swabs collected from healthy individuals in rural communities, 48 <em>S. aureus</em> isolates were recovered. Among these, 16.67% (8/48) isolates were identified as methicillin-resistant <em>S. aureus</em> (MRSA), resulting in an overall MRSA carriage rate of 1.24% (8/646); 75% (36/48) of the isolates were identified as multidrug-resistant, and the resistance rates to penicillin and erythromycin were relatively high, at 95.83% (46/48) and 91.67% (44/48) respectively. In total, 23 <em>spa</em> types and 12 MLSTs were identified, with t34, ST398, and ST59 being the most prevalent. Dominant MRSA clones included ST59-t437-IVa and ST9-t899-XII. Phylogenetic analysis revealed close genetic relatedness between local isolates and strains of porcine origin or those previously reported in Germany, indicating potential interspecies and transboundary transmission pathways.</div></div><div><h3>Conclusion</h3><div>The findings highlighted a concerning <em>S. aureus</em> AMR burden among healthy rural populations in China and provided molecular evidence supporting the existence of animal-environment-human transmission routes. These results underscored critical gaps in current One Health frameworks and called for urgent, integrated interventions to mitigate the complex and interconnected nature of AMR threats.</div></div>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"45 ","pages":"Pages 41-46"},"PeriodicalIF":3.2,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144859192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-12-01Epub Date: 2025-09-27DOI: 10.1016/j.jgar.2025.09.012
Jinhua Zhang , Yi Liu , Lei Fang , Xinrui Wang , Hao Xu , Danfeng Lou
Objectives
This study aims to elucidate the molecular characteristics of a blaVIM-2-carrying Pseudomonas asiatica isolate (L2126) from China and to characterize a ∼44 kb untypable plasmid harboring blaVIM-2. We investigated the genetic context of blaVIM-2, assessed the associated antimicrobial resistance determinants, and explored the role of this plasmid in mediating gene dissemination.
Methods
The isolate L2126 was recovered from an intestinal colonization sample in a patient from Hangzhou, China. Species identification was confirmed by average nucleotide identity (ANI) analysis. Hybrid whole-genome sequencing was performed using Illumina short-read and Oxford Nanopore long-read platforms. Genome assembly was conducted using Unicycler and annotated with Prokka. Antimicrobial resistance genes were identified via ResFinder and CARD. The genetic context of blaVIM-2 was delineated using IntegronFinder. Plasmid profiles were determined by S1-nuclease pulsed-field gel electrophoresis (S1-PFGE) and in silico replicon analysis.
Results
L2126 exhibited a multidrug-resistant profile with high-level resistance to carbapenems, cephalosporins, and fluoroquinolones. Genome analysis revealed 7 resistance genes, including blaVIM-2 and sul1. Notably, blaVIM-2 resides within a class 1 integron (intI1-attI1-blaVIM-2-qacEΔ1-sul1) embedded in a Tn402-like platform on a ∼44 kb untypable plasmid. The adjacent tni module (tniR-tniQ-tniB-tniA) is encoded on the opposite strand, indicating that it is part of the transposition platform rather than the integron cassette array. S1-PFGE confirmed the presence of the ∼44 kb plasmid, and in silico analysis provided a schematic representation of its genetic organization. BLAST analysis demonstrated that this plasmid shares high sequence homology with a plasmid previously identified in Pseudomonas monteilii, despite the two isolates belonging to different species.
Conclusions
Our findings demonstrate that the carriage of blaVIM-2 on a novel ∼44 kb untypable plasmid in P. asiatica L2126 could facilitate horizontal gene transfer of carbapenem resistance. The plasmid’s high homology to one previously identified in P. monteilii suggests that it has the potential for intra-genus dissemination, posing a significant threat to the spread of carbapenem resistance.
{"title":"Molecular characterization of blaVIM-2-carrying Pseudomonas asiatica L2126: identification of a ∼44 kb untypable plasmid with intra-genus dissemination potential","authors":"Jinhua Zhang , Yi Liu , Lei Fang , Xinrui Wang , Hao Xu , Danfeng Lou","doi":"10.1016/j.jgar.2025.09.012","DOIUrl":"10.1016/j.jgar.2025.09.012","url":null,"abstract":"<div><h3>Objectives</h3><div>This study aims to elucidate the molecular characteristics of a <em>bla</em><sub>VIM-2</sub>-carrying <em>Pseudomonas asiatica</em> isolate (L2126) from China and to characterize a ∼44 kb untypable plasmid harboring <em>bla</em><sub>VIM-2</sub>. We investigated the genetic context of <em>bla</em><sub>VIM-2</sub>, assessed the associated antimicrobial resistance determinants, and explored the role of this plasmid in mediating gene dissemination.</div></div><div><h3>Methods</h3><div>The isolate L2126 was recovered from an intestinal colonization sample in a patient from Hangzhou, China. Species identification was confirmed by average nucleotide identity (ANI) analysis. Hybrid whole-genome sequencing was performed using Illumina short-read and Oxford Nanopore long-read platforms. Genome assembly was conducted using Unicycler and annotated with Prokka. Antimicrobial resistance genes were identified via ResFinder and CARD. The genetic context of <em>bla</em><sub>VIM-2</sub> was delineated using IntegronFinder. Plasmid profiles were determined by S1-nuclease pulsed-field gel electrophoresis (S1-PFGE) and <em>in silico</em> replicon analysis.</div></div><div><h3>Results</h3><div>L2126 exhibited a multidrug-resistant profile with high-level resistance to carbapenems, cephalosporins, and fluoroquinolones. Genome analysis revealed 7 resistance genes, including <em>bla</em><sub>VIM-2</sub> and <em>sul1</em>. Notably, <em>bla</em><sub>VIM-2</sub> resides within a class 1 integron (<em>intI1-attI1-bla</em><sub>VIM-2</sub><em>-qacEΔ1-sul1</em>) embedded in a Tn<em>402</em>-like platform on a ∼44 kb untypable plasmid. The adjacent <em>tni</em> module (<em>tniR-tniQ-tniB-tniA</em>) is encoded on the opposite strand, indicating that it is part of the transposition platform rather than the integron cassette array. S1-PFGE confirmed the presence of the ∼44 kb plasmid, and <em>in silico</em> analysis provided a schematic representation of its genetic organization. BLAST analysis demonstrated that this plasmid shares high sequence homology with a plasmid previously identified in <em>Pseudomonas monteilii</em>, despite the two isolates belonging to different species.</div></div><div><h3>Conclusions</h3><div>Our findings demonstrate that the carriage of <em>bla</em><sub>VIM-2</sub> on a novel ∼44 kb untypable plasmid in <em>P. asiatica</em> L2126 could facilitate horizontal gene transfer of carbapenem resistance. The plasmid’s high homology to one previously identified in <em>P. monteilii</em> suggests that it has the potential for intra-genus dissemination, posing a significant threat to the spread of carbapenem resistance.</div></div>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"45 ","pages":"Pages 173-175"},"PeriodicalIF":3.2,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145191761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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