Journal of global antimicrobial resistance最新文献

{"title":"Serotypes and antimicrobial resistance patterns of food-borne Salmonella isolates in the Jiaxing Region, China, from 2020 to 2024","authors":"Xiaoqin Niu ,&nbsp;Liping Tu ,&nbsp;Qinlong Yu ,&nbsp;Binbin Lu ,&nbsp;Junhua Tian ,&nbsp;Wei Wang ,&nbsp;Bailong Hou ,&nbsp;Weifeng Shen","doi":"10.1016/j.jgar.2025.10.023","DOIUrl":"10.1016/j.jgar.2025.10.023","url":null,"abstract":"<div><h3>Background</h3><div><em>Salmonella</em> is a worldwide food-borne pathogen, which brings significant challenges to clinical practice due to the increase of antibiotic resistance.</div></div><div><h3>Objective</h3><div>This study aims to explore the serotype variety and antibiotic resistance of food-borne <em>Salmonella</em> in the Jiaxing region from 2020 to 2024.</div></div><div><h3>Methods</h3><div>The faecal samples of 8541 patients with food-borne diseases were investigated. The isolated <em>Salmonella</em> strains underwent MALDI-TOF, serological typing, and drug susceptibility testing.</div></div><div><h3>Results</h3><div>A total of 622 <em>Salmonella</em> strains were isolated from 8541 faecal samples, with 382 (61.4%) originating from patients under 6 y old. Summer and autumn were the peak seasons. 622 strains of <em>Salmonella</em> were divided into 53 serotypes, with the most common serotypes being <em>Salmonella</em> Typhimurium and <em>Salmonella</em> Enteritidis. The drug susceptibility test revealed that most strains were resistant to ampicillin (79.8%), while 38% exhibited resistance to trimethoprim/sulfamethoxazole. The susceptibility rate to cephalosporins and fluoroquinolones was over 84%. Only one strain was found to be resistant to carbapenems. Notably, 69.5% of the strains were resistant to at least one antibiotic, and 21.9% demonstrated multidrug resistance. Our findings also indicated an increasing trend in the resistance of isolated strains to ceftriaxone and cefepime over the past 5 y. Compared to <em>S</em>. Typhimurium, <em>S</em>. Enteritidis was more susceptible to a range of antibiotics, with the most notable difference in susceptibility to trimethoprim/sulfamethoxazole.</div></div><div><h3>Conclusions</h3><div><em>Salmonella</em> serotypes exhibited varied patterns of drug susceptibility. Antibiotic selection should be based on the susceptibility profile of the pathogenic bacteria to effectively treat and prevent <em>Salmonella</em> infections.</div></div>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"45 ","pages":"Pages 294-298"},"PeriodicalIF":3.2,"publicationDate":"2025-10-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145401066","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomic characterization of a carbapenem-resistant Citrobacter freundii complex strain co-carrying blaNDM-1, blaCTX-M-30, armA, rmtC, and rmtD from Chile","authors":"Isidora Manríquez-Cuadra ,&nbsp;Maximiliano Matus-Köhler ,&nbsp;Alejandro Aguayo-Reyes ,&nbsp;Mario Quezada-Aguiluz ,&nbsp;Felipe Morales-Leon ,&nbsp;Gerardo González-Rocha ,&nbsp;Andrés Opazo-Capurro","doi":"10.1016/j.jgar.2025.10.021","DOIUrl":"10.1016/j.jgar.2025.10.021","url":null,"abstract":"<div><h3>Introduction</h3><div>Carbapenem- and third-generation cephalosporin-resistant <em>Enterobacterales</em>, including the <em>Citrobacter freundii</em> complex, are classified as a World Health Organization ‘critical priority’ due to their public health threat. This study reports a <em>C. freundii</em> complex isolate (Cit-107) co-harbouring the <em>bla</em>_NDM-1, <em>bla</em>_CTX-M-30, <em>armA, rmtC</em>, and <em>rmtD</em> genes, recovered from a rectal swab in 2022 in Concepción, Chile.</div></div><div><h3>Methods</h3><div>Antimicrobial susceptibility was assessed via disk diffusion. Whole-genome sequencing was performed using the Illumina NextSeq 2000 platform and annotated with Bakta. <em>In silico</em> analyses included JSpeciesWS, PubMLST, ABRicate, AMRFinderPlus, and MOB-suite. Phylogenetic analysis of Cit-107 and 50 <em>C. freundii</em> complex genomes from the Americas was conducted using IQ-TREE and visualized with iTOL.</div></div><div><h3>Results</h3><div>Cit-107 was resistant to clinically relevant antibiotics, such as carbapenems, cephalosporins, aminoglycosides, and fluoroquinolones. It belongs to sequence type (ST) ST112 and carries multiple resistance genes: <em>bla</em>_NDM-1, <em>bla</em>_CTX-M-30, <em>mcr-9.1, armA, rmtC</em>, and <em>rmtD</em>. The <em>bla</em>_NDM-1 gene was embedded in a Tn<em>125</em>-like transposon contained in an IncC-type plasmid alongside the <em>bla</em>_CTX-M-30, <em>rmtC</em>, and <em>tet(G)</em> genes. The <em>bla</em>_CTX-M-30 was harboured in an IncN-type plasmid, while <em>armA, msr(E)</em>, and <em>mph(E)</em> were co-carried in an IncHI2A-type plasmid. Phylogenetic analysis revealed Cit-107 as a divergent lineage within the <em>C. freundii</em> complex.</div></div><div><h3>Conclusions</h3><div>This is the first report of a carbapenem-resistant <em>C. freundii</em> complex isolate carrying <em>bla</em>_NDM-1 in Chile alongside multiple antibiotic-resistance genes harboured in several plasmid types. Its unique resistome and phylogenetic profile underscore the need for surveillance of emerging multidrug-resistant <em>C. freundii</em> strains in clinical settings.</div></div>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"45 ","pages":"Pages 290-293"},"PeriodicalIF":3.2,"publicationDate":"2025-10-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145401026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"in vitro antibacterial activity of nordihydroguaiaretic acid combined with ceftriaxone against carbapenem-resistant Klebsiella pneumoniae","authors":"Chengjiao Luo ,&nbsp;Hua Tan ,&nbsp;Hang Sun ,&nbsp;Xianwei Zhang ,&nbsp;Qian Chen","doi":"10.1016/j.jgar.2025.10.007","DOIUrl":"10.1016/j.jgar.2025.10.007","url":null,"abstract":"<div><h3>Objective</h3><div>This study investigated the interplay between biofilm formation and efflux pumps in carbapenem-resistant <em>Klebsiella pneumoniae</em> (CRKP) and evaluated the biofilm inhibition by nordihydroguaiaretic acid (NDGA) along with the efficacy of its combination with ceftriaxone (CRO) against CRKP, to inform the development of novel therapeutic strategies against multidrug-resistant infections.</div></div><div><h3>Methods</h3><div>Efflux pump genes were detected via PCR. Biofilm formation was quantified by crystal violet staining and categorized by the biofilm formation index (BFI) classification. Strong biofilm producers (BFI &gt; 4 × negative control) were selected for <em>in vitro</em> models. Efflux pump gene expression was compared between biofilm and planktonic states using qRT-PCR. NDGA’s biofilm inhibition and NDGA-CRO synergy were assessed via checkerboard assays.</div></div><div><h3>Results</h3><div>Among the 86 CRKP isolates, 51.16% (44/86) were classified as strong biofilm producers, followed by moderate (17.44%, 15/86), weak (20.93%, 18/86), and negative (10.47%, 9/86) producers. Efflux pump genes (<em>acrA, acrB, tolC, oqxA, oqxB</em>) were detected in 100%, 100%, 100%, 97.7%, and 98.84% of isolates. Gene expression was significantly upregulated in biofilm versus planktonic state (<em>P</em> &lt; 0.05). NDGA (≥16 µg/mL) inhibited biofilm formation, and its combination with CRO exhibited additive (63.64%) and synergistic (36.36%) effects.</div></div><div><h3>Conclusion</h3><div>Efflux pumps and biofilms synergistically enhance CRKP resistance. By inhibiting both efflux-mediated antibiotic extrusion and biofilm formation, NDGA resensitizes CRKP to CRO. This <em>in vitro</em> efficacy supports further development, pending <em>in vivo</em> safety studies in animal models.</div></div>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"46 ","pages":"Pages 31-37"},"PeriodicalIF":3.2,"publicationDate":"2025-10-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145401086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence and molecular epidemiology of optrA-positive Enterococcus in herders and yaks from high-altitude Tibet, China","authors":"Hanyu Wang ,&nbsp;Xinyue Han ,&nbsp;Aoxiao Chen ,&nbsp;Zelin Yan ,&nbsp;Rong Zhang ,&nbsp;Yang Wang","doi":"10.1016/j.jgar.2025.10.016","DOIUrl":"10.1016/j.jgar.2025.10.016","url":null,"abstract":"<div><h3>Objectives</h3><div>Antimicrobial resistance (AMR) in high-altitude environments remains understudied despite its growing global concern. This study investigates the molecular epidemiology of the <em>optrA</em> gene, which confers resistance to oxazolidinones and phenicols, in <em>Enterococcus</em> species isolated from herders and yak faecal samples in Nagqu, Tibet, China.</div></div><div><h3>Methods</h3><div>A total of 161 faecal samples from herders and 42 from yaks were collected and analysed for the presence of <em>optrA</em>-positive <em>Enterococcus</em> strains. Antimicrobial susceptibility testing (AST) was performed to determine resistance patterns. Whole-genome sequencing (WGS) and phylogenetic analysis were used to assess genetic relationships and the potential for clonal spread and horizontal gene transfer.</div></div><div><h3>Results</h3><div>The <em>optrA</em> gene was detected in 6.2% (10/161) of human samples and 9.5% (4/42) of yak samples, indicating a higher prevalence in yaks. Resistance was highest for tetracycline (100%), erythromycin (92.9%), and linezolid (92.9%). Phylogenetic analysis revealed clonal dissemination, with some isolates exhibiting high genetic homology. Notably, one <em>E. faecalis</em> strain belonged to ST16, a sequence type commonly found in low-altitude cities, suggesting potential transmission between regions. The <em>optrA</em> gene was frequently associated with mobile genetic elements, indicating a risk of horizontal gene transfer and further dissemination of resistance.</div></div><div><h3>Conclusion</h3><div>The presence of <em>optrA</em>-positive <em>Enterococcus</em> in both human and animal populations in this high-altitude region underscores the role of human-animal interactions in AMR transmission. The increasing prevalence of resistant strains in yaks, coupled with genetic evidence of clonal expansion, highlights the need for a comprehensive One Health approach to AMR surveillance and mitigation in remote, high-altitude environments.</div></div>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"46 ","pages":"Pages 1-5"},"PeriodicalIF":3.2,"publicationDate":"2025-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145370299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ceftolozane/tazobactam in action: Prescribing patterns, effectiveness, and healthcare utilisation across seven countries","authors":"Emre Yucel ,&nbsp;Alex Soriano ,&nbsp;David L. Paterson ,&nbsp;Florian Thalhalmmer ,&nbsp;Stefan Kluge ,&nbsp;Pierluigi Viale ,&nbsp;Mike Allen ,&nbsp;Brune Akrich ,&nbsp;Jessica Levy ,&nbsp;Huina Yang ,&nbsp;Sunny Kaul","doi":"10.1016/j.jgar.2025.10.013","DOIUrl":"10.1016/j.jgar.2025.10.013","url":null,"abstract":"<div><h3>Objectives</h3><div>Infections by antimicrobial-resistant, Gram-negative bacteria present significant global challenge, resulting in difficult treatment scenarios and poor clinical outcomes. Although ceftolozane/tazobactam (C/T) has been approved for the treatment of hospital-acquired HABP/VABP, cUTI, and cIAI, data on real-world use remains limited. This study presents real-world use of C/T across various countries.</div></div><div><h3>Methods</h3><div>The Study of Prescribing patterns and Effectiveness of Ceftolozane/Tazobactam: Real-world Analysis (SPECTRA) was a retrospective, observational study involving 617 adult inpatients across seven countries who were treated with C/T for at least 48 hours between 2016 and 2020. The primary objective was to describe real-world utilisation of C/T, focusing on sociodemographic and clinical characteristics, treatment patterns, clinical outcomes, and resource utilisation.</div></div><div><h3>Results</h3><div>617 patients from seven countries were included. Prevalence of at least one comorbidity ranged from 55.9% (Mexico) to 91.7% (Austria), with mean number of comorbidities from 1.0 (Mexico) to 2.7 (UK). Culture results from 72.7% patients identified MDR <em>Pseudomonas aeruginosa</em>, ranging from 55.9% (Italy) to 80.6% (Spain). Median hospital length of stay ranged from 23 (Mexico) to 53.5 (Austria) days. Clinical success rate for treating the index infection with C/T was 64.8%, with 51.1% of cases treated within the ICU.</div></div><div><h3>Conclusions</h3><div>This multi-national, retrospective, observational study demonstrates the real-world use and healthcare resource utilisation (HCRU) of C/T in patients with Gram-negative infections across seven countries. These findings provide valuable insights into prescribing patterns and resource implications of C/T treatment, underscoring the importance of real-world data to inform clinical practice and optimise antimicrobial therapy in diverse healthcare settings.</div></div>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"46 ","pages":"Pages 14-21"},"PeriodicalIF":3.2,"publicationDate":"2025-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145623450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rapid clonal replacement by ST773 with NDM-1 among carbapenem-resistant Pseudomonas aeruginosa in South Korea","authors":"Yonggeun Cho ,&nbsp;Kyung-Wook Hong ,&nbsp;Min-Kyoung Shin ,&nbsp;Sunjoo Kim ,&nbsp;Jung-Hyun Byun","doi":"10.1016/j.jgar.2025.10.010","DOIUrl":"10.1016/j.jgar.2025.10.010","url":null,"abstract":"","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"45 ","pages":"Pages 287-289"},"PeriodicalIF":3.2,"publicationDate":"2025-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145370278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Elucidating artemisinin (ART)-heme as a biomarker for ART resistance and ATP-dependent transport of ART in Plasmodium falciparum","authors":"Hui Liu ,&nbsp;Hongchang Zhou ,&nbsp;Yuan Cai ,&nbsp;Shanshan Du ,&nbsp;Zhenghui Huang ,&nbsp;Kun Xu ,&nbsp;Jie Xing","doi":"10.1016/j.jgar.2025.10.017","DOIUrl":"10.1016/j.jgar.2025.10.017","url":null,"abstract":"<div><h3>Objectives</h3><div>Artemisinin (ART) resistance is widespread in Southeast Asia and emerging in Africa. In this study, the potential biomarker for ART resistance was first characterised in <em>P. falciparum</em>, and the roles of several molecular pathways (e.g., Hb endocytosis) in ART resistance were then evaluated.</div></div><div><h3>Methods</h3><div>The metabolic profiles of ART and its pharmacokinetics were studied in <em>Plasmodium falciparum (P. falciparum)</em> incubated <em>in vitro.</em> Several strains of <em>P. falciparum</em> (ART-sensitive strains <em>Pf</em>3D7/<em>Pf</em>Dd2, and ART-resistance strain <em>Pf</em>6320 carrying K13 mutation C580Y) synchronised at different stages (ring and trophozoite) were used. The antimalarial activity of ART-heme adduct was evaluated. Several molecular pathways (i.e., Hb endocytosis) were evaluated for their roles in ART resistance using (non)selective inhibitors.</div></div><div><h3>Results</h3><div>ART-heme adduct was identified as a major metabolite for ART in <em>P. falciparum</em>, and it exhibited weak antimalarial activity (µM level). In ART-sensitive parasites, the formation of ART-heme was rapid, and both time- and concentration-dependent (50–450 nM). In contrast, ART-heme formation in ART-resistant parasites was independent of ART concentration. ART-heme formation was susceptible to orthovanadate (an inhibitor of P-type ATPase). Hb endocytosis/degradation, SERCA and the efflux transporter played roles in ART-heme formation to some extent. Synergistic antiplasmodial effect existed between ART and verapamil (an inhibitor of the efflux transporter).</div></div><div><h3>Conclusions</h3><div>ART-heme adduct displayed weak antiplasmodial activity but may still serve as a biomarker for both the antiplasmodial efficacy of ART and its resistance. ART transport in <em>P. falciparum</em> was ATP-dependent, and inhibition of efflux transporter may be a strategy to enhance ART potency.</div></div>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"45 ","pages":"Pages 260-267"},"PeriodicalIF":3.2,"publicationDate":"2025-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145370315","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy and safety of novel β-lactam/β-lactamase inhibitor combinations for the treatment of complicated urinary tract infections or acute pyelonephritis: A systematic review and meta-analysis","authors":"Sirui Tang ,&nbsp;Yuxuan Song ,&nbsp;Caipeng Qin ,&nbsp;Tao Xu","doi":"10.1016/j.jgar.2025.10.011","DOIUrl":"10.1016/j.jgar.2025.10.011","url":null,"abstract":"<div><h3>Objectives</h3><div>The increasing resistance of gram-negative bacteria in complicated urinary tract infections (cUTI) and acute pyelonephritis (APN) poses major treatment challenges. This study aimed to evaluated the efficacy and safety of novel β-Lactam/β-Lactamase inhibitor combinations compared with conventional antibiotics.</div></div><div><h3>Methods</h3><div>We systematically searched PubMed, Embase, Cochrane Library, Web of Science, ClinicalTrials.gov, and FDA.gov for randomized controlled trials (RCTs) published up to July 15, 2025. Eligible studied included patients with cUTI or APN. Primary outcomes were clinical and microbiological response rates at the test-of-cure (TOC) or the end-of-treatment visit. Secondary outcomes included adverse events (AEs), serious AEs (SAEs), and treatment discontinuations due to AEs. Risk of bias was assessed using the Cochran tool.</div></div><div><h3>Results</h3><div>Eleven RCTs with a total of 4986 patients (2719 in the experimental group and 2267 in the control group) were included. Novel β-Lactam/β-Lactamase inhibitors significantly improved clinical response in the microbiological modified intent-to-treat population (OR = 1.64, 95% CI [1.43–1.88], <em>P</em> &lt; 0.001). The incidence of overall AEs and SAEs was similar between groups, though drug-related AEs were more common in the experimental group (OR = 1.38, 95% CI [1.11–1.72], <em>P</em> = 0.003).</div></div><div><h3>Conclusions</h3><div>Novel β-Lactam/β-Lactamase inhibitor combinations demonstrated superior efficacy and comparable safety to conventional antibiotics in treating cUTI and APN, particularly at the TOC stage.</div></div>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"45 ","pages":"Pages 268-281"},"PeriodicalIF":3.2,"publicationDate":"2025-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145368125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antimicrobial susceptibility patterns and genotypic characteristics of Mycobacterium marinum and Mycobacterium abscessus isolated from cutaneous infections: A retrospective study","authors":"Jiayi Peng ,&nbsp;Wenyue Zhang ,&nbsp;Ying Shi ,&nbsp;Haiqin Jiang ,&nbsp;Jingshu Xiong ,&nbsp;Youming Mei ,&nbsp;Tian Gan ,&nbsp;Hongsheng Wang","doi":"10.1016/j.jgar.2025.10.001","DOIUrl":"10.1016/j.jgar.2025.10.001","url":null,"abstract":"<div><h3>Objectives</h3><div>Antimicrobial susceptibility data for cutaneous mycobacteria remain limited. This study investigated the antimicrobial susceptibility patterns and molecular resistance mechanisms of <em>Mycobacterium marinum</em> and <em>Mycobacterium abscessus</em> isolates from a dermatology specialized hospital in China.</div></div><div><h3>Methods</h3><div>Antimicrobial susceptibility testing was performed on 200 <em>M. marinum</em> and 50 <em>M. abscessus</em> clinical isolates using broth microdilution method. <em>M. abscessus</em> subspecies were identified through <em>hsp65, erm (41),</em> and <em>rpoB</em> gene sequencing. For <em>M. abscessus</em> isolates, resistance-associated mutations for clarithromycin, amikacin, and fluoroquinolones were analysed by sequencing <em>erm (41), rrl, rrs, gyrA, and gyrB</em> genes.</div></div><div><h3>Results</h3><div><em>M. marinum</em> demonstrated high susceptibility (82.5%–100%) to clarithromycin, rifampin, rifabutin, moxifloxacin, linezolid, trimethoprim-sulfamethoxazole, with moderate susceptibility to tetracyclines and ciprofloxacin. Ethambutol showed favourable activity against <em>M. marinum</em> with MIC₉₀ of 2 µg/mL. Among <em>M. abscessus</em> isolates (23 <em>M. abscessus subsp. abscessus</em>, 26 <em>M. abscessus subsp. massiliense</em>, 1 <em>M. abscessus subsp. bolletii</em>), overall susceptibility to clarithromycin and amikacin was 78% and 82%, respectively. Tigecycline and clofazimine were effective against <em>M. abscessus</em> with MIC₉₀ 1 and 0.5 µg/mL, respectively. In contrast, <em>M. abscessus</em> isolates demonstrated high-level of resistance to multiple antibiotics, including linezolid, fluoroquinolones, and tetracyclines. <em>M. abscessus subsp. massiliense</em> exhibited higher clarithromycin susceptibility (100%) compared to <em>M. abscessus subsp. abscessus</em> (56.5%). Clarithromycin resistance of <em>M. abscessus</em> isolates correlated with functional T28 sequevar in the <em>erm (41)</em> gene.</div></div><div><h3>Conclusions</h3><div>Our findings elucidate distinct antimicrobial susceptibility profiles of <em>M. marinum</em> and <em>M. abscessus</em> isolated from cutaneous infection in China, providing critical guidance for clinical treatment. Cutaneous <em>M. abscessus</em> isolates exhibit extensive drug resistance patterns. Subtyping and <em>erm (41)</em> polymorphism detection serve as reliable predictors of clarithromycin resistance in <em>M. abscessus</em>.</div></div>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"45 ","pages":"Pages 240-247"},"PeriodicalIF":3.2,"publicationDate":"2025-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145368143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"blaNDM-5 is the predominant variant of blaNDM gene in Klebsiella species detected in Mississippi, USA","authors":"Srimadhav Nallani ,&nbsp;Saihou Ceesay ,&nbsp;Abiye Iyo,&nbsp;Lucersia Nichols,&nbsp;Daphne Ware,&nbsp;Beata Karolewicz","doi":"10.1016/j.jgar.2025.10.014","DOIUrl":"10.1016/j.jgar.2025.10.014","url":null,"abstract":"<div><h3>Objectives</h3><div>The Mississippi Public Health Laboratory conducts active surveillance of carbapenem-resistant <em>Enterobacterales</em> (CRE) infections as part of the Antimicrobial Resistance Laboratory Network (ARLN) program implemented by the United States Centers for Disease Control and Prevention (CDC). Here, we describe the prevalence of New Delhi Metallo-β-lactamase (<em>bla</em>_NDM) genes in <em>Klebsiella</em>, a major pathogen of the <em>Enterobacterales</em> order linked to nosocomial infections, between January 2022 and June 2025.</div></div><div><h3>Methods</h3><div>Carbapenemase activity was confirmed using the Modified Carbapenem Inactivation Method (mCIM). PCR was then performed on carbapenemase-positive isolates to detect <em>bla</em>_NDM genes. Whole-genome sequencing (WGS) was performed on 35 <em>bla</em>_NDM-positive isolates to identify <em>bla</em>_NDM gene variants, other antimicrobial resistance (AMR) determinants, virulence factors, and plasmids. Antibiotic susceptibility testing (AST) was determined by the Kirby-Bauer disk diffusion assay.</div></div><div><h3>Results</h3><div>Among the 35 NDM-positive <em>Klebsiella</em> species, the <em>bla</em>_NDM-5 variant was identified in 23 (65.7%) isolates, followed by <em>bla</em>_NDM-1 and <em>bla</em>_NDM-7 genes which were identified in 6/35 (17%) and 6/35 (17%) cases, respectively. A significant proportion of <em>bla</em>_NDM-5 cases also harbored additional β-lactamase genes such as <em>bla</em>_SHV family (18/23; 78%) and <em>bla</em>_TEM-1 (9/23; 39%) indicating multi-drug resistance. All 23 <em>bla</em>_NDM-5-positive isolates were resistant to carbapenems and broad-spectrum cephalosporins, but 13/23 (56.5%) remained susceptible to aztreonam. PlasmidFinder analysis identified IncX3 (20/23) and IncFIB(K) (19/23) as the predominant plasmid replicons among these isolates.</div></div><div><h3>Conclusion</h3><div>The emergence and spread of bacteria carrying <em>bla</em>_NDM-5 gene poses a significant threat to public health in Mississippi. Our dataset warrants further study to investigate the potential role of plasmids in <em>bla</em>_NDM-5 dissemination.</div></div>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"45 ","pages":"Pages 282-286"},"PeriodicalIF":3.2,"publicationDate":"2025-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145354938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}