Tlehema Gwandu Umbayda, Anthony Daniel Funga, Alinanuswe Joel Mwakalesi
A significant portion of globally produced fruits are lost between the farmer and consumer, necessitating solutions like natural edible coatings for preservation due to their low cost and less toxicity. This study aimed to assess whether the combination of macadamia nut oil and chitosan posed synergistic effects on physicochemical properties of tomato fruits or the macadamia nut oil alone. A completely randomized design (CRD) with eight treatments was employed: S1 (control), S2–S4 (combined macadamia nut oil and chitosan coating), S5–S7 (macadamia nut oil coating), and S8 (chitosan). The experimental setup was conducted over 20 days at refrigeration (4°C) and postharvest shed conditions (23.8°C–30°C, 65.8%–97.5% RH). The coating solution made up of a combination of 2.5% v/v macadamia nut oil and 1% w/v chitosan showed significant differences (p ≤ 0.05) in slowing down the changes in pH from 3.95 to 4.3, total chlorophyll content from 0.19 mg/100 to 0.12 mg/100 mL, and lipid peroxidation from 13.5 to 19.71 meq/kg. Moreover, coating solutions made by macadamia nut oil alone, especially with 1% v/v, showed the smallest increases in total soluble solids (TSS) and total sugar content, and also they exhibited the smallest decreases in titratable acidity (0.64 to 0.08 g/L), ripening index (5.78 to 84.74), total carotenoids (17.02 to 24.585 μg/g), and firmness (355 N to 130 N). Comparative analysis indicated that 1% v/v macadamia nut oil had higher mean differences (p < 0.05) for most physicochemical parameters than coating solutions comprising 2.5% v/v macadamia nut oil and 1% w/v chitosan. However, this finding highlighted that the combination of macadamia nut oil with chitosan offers a synergistic effect on specific crucial parameters (pH, total chlorophyll content, and lipid peroxidation) compared to macadamia nut oil alone. Further studies could investigate the antimicrobial effect of tomatoes treated with a combination of macadamia nut oil and chitosan.
{"title":"Synergistic Effect of Macadamia Nut Oil and Chitosan Coatings on Physicochemical Characteristics of Tomatoes During Storage","authors":"Tlehema Gwandu Umbayda, Anthony Daniel Funga, Alinanuswe Joel Mwakalesi","doi":"10.1155/jfq/1474478","DOIUrl":"https://doi.org/10.1155/jfq/1474478","url":null,"abstract":"<p>A significant portion of globally produced fruits are lost between the farmer and consumer, necessitating solutions like natural edible coatings for preservation due to their low cost and less toxicity. This study aimed to assess whether the combination of macadamia nut oil and chitosan posed synergistic effects on physicochemical properties of tomato fruits or the macadamia nut oil alone. A completely randomized design (CRD) with eight treatments was employed: S1 (control), S2–S4 (combined macadamia nut oil and chitosan coating), S5–S7 (macadamia nut oil coating), and S8 (chitosan). The experimental setup was conducted over 20 days at refrigeration (4°C) and postharvest shed conditions (23.8°C–30°C, 65.8%–97.5% RH). The coating solution made up of a combination of 2.5% v/v macadamia nut oil and 1% w/v chitosan showed significant differences (<i>p</i> ≤ 0.05) in slowing down the changes in pH from 3.95 to 4.3, total chlorophyll content from 0.19 mg/100 to 0.12 mg/100 mL, and lipid peroxidation from 13.5 to 19.71 meq/kg. Moreover, coating solutions made by macadamia nut oil alone, especially with 1% v/v, showed the smallest increases in total soluble solids (TSS) and total sugar content, and also they exhibited the smallest decreases in titratable acidity (0.64 to 0.08 g/L), ripening index (5.78 to 84.74), total carotenoids (17.02 to 24.585 μg/g), and firmness (355 N to 130 N). Comparative analysis indicated that 1% v/v macadamia nut oil had higher mean differences (<i>p</i> < 0.05) for most physicochemical parameters than coating solutions comprising 2.5% v/v macadamia nut oil and 1% w/v chitosan. However, this finding highlighted that the combination of macadamia nut oil with chitosan offers a synergistic effect on specific crucial parameters (pH, total chlorophyll content, and lipid peroxidation) compared to macadamia nut oil alone. Further studies could investigate the antimicrobial effect of tomatoes treated with a combination of macadamia nut oil and chitosan.</p>","PeriodicalId":15951,"journal":{"name":"Journal of Food Quality","volume":"2025 1","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jfq/1474478","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145572168","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mst. Sadnima Zaman, Alima Khanam, Md. Rezoan Hossain, Syed Rashel Kabir, A. K. M. Asaduzzaman, Imtiaj Hasan
Lectins are glycan-binding proteins that have been extensively studied for their diverse biological functions, including potential therapeutic applications. This study aimed to purify and characterize chitin-binding lectins from two Solanaceae plants, Solanum tuberosum and Solanum annuum, and evaluate their in vivo antitumor activities against Ehrlich ascites carcinoma (EAC) cells in Swiss albino mice. The lectins, designated as Solanum tuberosum lectin (STL) and Solanum annuum lectin (SAL), were purified using affinity chromatography on an acetylated chitin column. Their molecular weights ranged from 12 to 66 kDa. Both lectins exhibited hemagglutination activity and demonstrated stability across a range of temperatures and pH levels. Carbohydrate-binding specificity assays showed that STLs had a preference for glucose and N-acetyl-D-glucosamine, while SALs bound specifically to mannose, glucose, and methyl-α-D-galactopyranoside. STLs were found to be metal-dependent, in contrast to the metal-independent nature of SALs. Toxicity was assessed using the brine shrimp lethality assay, revealing moderate toxicity levels, with LC50 values of 154.36 μg/mL for STLs and 380.45 μg/mL for SALs. In vivo antitumor activity was determined through intraperitoneal administration of lectins to EAC-bearing mice. STLs inhibited tumor growth by 57.85% and 73.57% at doses of 2 and 4 mg/kg/day, respectively, whereas SALs achieved tumor inhibition rates of 32.86% and 58.57% at the same doses. These antitumor effects were supported by favorable changes in hematological parameters in lectin-treated mice. The results indicate that chitin-binding lectins from Solanum tuberosum and Solanum annuum possess promising anticancer properties, with STLs exhibiting comparatively higher efficacy. The inhibition of EAC cell growth was attributed to apoptosis induction, as evidenced by morphological changes, upregulation of Bax and PARP, and the downregulation of Bcl-2 expression. Further research is warranted to elucidate their molecular mechanisms of action and evaluate their potential as novel therapeutic agents in cancer treatment.
凝集素是一种聚糖结合蛋白,因其多种生物学功能而被广泛研究,包括潜在的治疗应用。本研究旨在从茄科植物龙苏(Solanum tuberosum)和龙苏(Solanum annuum)中分离纯化几丁质结合凝集素,并对其在瑞士白化小鼠体内抗埃利希腹水癌(EAC)细胞的活性进行研究。采用乙酰化几丁质柱亲和层析纯化了两种凝集素,分别命名为龙葵凝集素(STL)和龙葵凝集素(SAL)。它们的分子量在12 ~ 66 kDa之间。两种凝集素都表现出血凝活性,并在一定温度和pH值范围内表现出稳定性。碳水化合物结合特异性分析表明,STLs对葡萄糖和n -乙酰- d -氨基葡萄糖有偏好,而SALs对甘露糖、葡萄糖和甲基α- d -半乳糖苷有特异性结合。与SALs不依赖金属的性质相反,stl被发现是金属依赖的。采用盐水对虾致死性试验评估其毒性,stl的LC50值为154.36 μg/mL, SALs的LC50值为380.45 μg/mL。通过腹腔注射凝集素对eac小鼠进行体内抗肿瘤活性测定。stl在2和4 mg/kg/d剂量下对肿瘤生长的抑制率分别为57.85%和73.57%,而SALs在相同剂量下的肿瘤抑制率分别为32.86%和58.57%。这些抗肿瘤作用得到了凝集素处理小鼠血液参数有利变化的支持。结果表明,从龙葵和龙葵中提取的几丁质结合凝集素具有良好的抗癌作用,其中STLs的抗癌效果较好。EAC对细胞生长的抑制与诱导凋亡有关,表现为形态学改变,Bax和PARP表达上调,Bcl-2表达下调。需要进一步的研究来阐明它们的分子作用机制,并评估它们作为新型治疗药物在癌症治疗中的潜力。
{"title":"A Comparative Study of the Anticancer Potential of Chitin-Binding Lectins From Solanum tuberosum and Solanum annuum","authors":"Mst. Sadnima Zaman, Alima Khanam, Md. Rezoan Hossain, Syed Rashel Kabir, A. K. M. Asaduzzaman, Imtiaj Hasan","doi":"10.1155/jfq/5568846","DOIUrl":"https://doi.org/10.1155/jfq/5568846","url":null,"abstract":"<p>Lectins are glycan-binding proteins that have been extensively studied for their diverse biological functions, including potential therapeutic applications. This study aimed to purify and characterize chitin-binding lectins from two Solanaceae plants, <i>Solanum tuberosum</i> and <i>Solanum annuum</i>, and evaluate their in vivo antitumor activities against Ehrlich ascites carcinoma (EAC) cells in Swiss albino mice. The lectins, designated as <i>Solanum tuberosum</i> lectin (STL) and <i>Solanum annuum</i> lectin (SAL), were purified using affinity chromatography on an acetylated chitin column. Their molecular weights ranged from 12 to 66 kDa. Both lectins exhibited hemagglutination activity and demonstrated stability across a range of temperatures and pH levels. Carbohydrate-binding specificity assays showed that STLs had a preference for glucose and N-acetyl-D-glucosamine, while SALs bound specifically to mannose, glucose, and methyl-α-D-galactopyranoside. STLs were found to be metal-dependent, in contrast to the metal-independent nature of SALs. Toxicity was assessed using the brine shrimp lethality assay, revealing moderate toxicity levels, with LC<sub>50</sub> values of 154.36 μg/mL for STLs and 380.45 μg/mL for SALs. In vivo antitumor activity was determined through intraperitoneal administration of lectins to EAC-bearing mice. STLs inhibited tumor growth by 57.85% and 73.57% at doses of 2 and 4 mg/kg/day, respectively, whereas SALs achieved tumor inhibition rates of 32.86% and 58.57% at the same doses. These antitumor effects were supported by favorable changes in hematological parameters in lectin-treated mice. The results indicate that chitin-binding lectins from <i>Solanum tuberosum</i> and <i>Solanum annuum</i> possess promising anticancer properties, with STLs exhibiting comparatively higher efficacy. The inhibition of EAC cell growth was attributed to apoptosis induction, as evidenced by morphological changes, upregulation of Bax and PARP, and the downregulation of Bcl-2 expression. Further research is warranted to elucidate their molecular mechanisms of action and evaluate their potential as novel therapeutic agents in cancer treatment.</p>","PeriodicalId":15951,"journal":{"name":"Journal of Food Quality","volume":"2025 1","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jfq/5568846","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145581319","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Cong Kha Nguyen, Thao Nhu Y. Pham, Ngoc Duc Vu, Long Giang Bach, Thanh Truc Tran
Jackfruit processing generates a large volume of by-products, which are often discarded despite their nutritional potential. At the same time, probiotic-based products are increasingly valued for their ability to support gut health, enhance immunity, and promote sustainable diets. This study aimed to develop probiotic granules using jackfruit by-products pulp, rag, and seeds combined with Lactobacillus plantarum. A series of formulations were systematically evaluated for granulation performance, physicochemical properties, and bacterial viability. The optimal formulation (pulp: rag 60: 40%, seed: wheat flour 50: 50%, and powder: sucrose 60: 40%) produced granules with good flowability (Carr index ∼7.8), stable color (L∗ ≈ 80, a∗ ≈ 2.5, and b∗ ≈ 18.5), and high probiotic survival above 6.4 logCFU/g after drying, thereby meeting the functional threshold for probiotic foods. Predrying the pulp:rag mixture to 60% moisture improved the recovery yield (∼59%), reduced drying time, and enhanced bacterial viability, while the 60:40 ratio of Mixture B (flour) to sucrose offered the best balance between sweetness, color quality, and probiotic stability. Drying at 60°C was identified as the most suitable condition to preserve both product quality and viability (> 7 logCFU/g), whereas higher temperatures led to a significant decline in bacterial counts. These results demonstrate that jackfruit by-products can be effectively transformed into stable, consumer-ready probiotic granules, offering both functional health benefits and a sustainable approach to reducing food waste.
{"title":"Development of a Probiotic Granule Formula Utilizing By-Products (Rag, Seed, and Pulp) From Jackfruit (Artocarpus heterophyllus L.)","authors":"Cong Kha Nguyen, Thao Nhu Y. Pham, Ngoc Duc Vu, Long Giang Bach, Thanh Truc Tran","doi":"10.1155/jfq/5333730","DOIUrl":"https://doi.org/10.1155/jfq/5333730","url":null,"abstract":"<p>Jackfruit processing generates a large volume of by-products, which are often discarded despite their nutritional potential. At the same time, probiotic-based products are increasingly valued for their ability to support gut health, enhance immunity, and promote sustainable diets. This study aimed to develop probiotic granules using jackfruit by-products pulp, rag, and seeds combined with <i>Lactobacillus plantarum</i>. A series of formulations were systematically evaluated for granulation performance, physicochemical properties, and bacterial viability. The optimal formulation (pulp: rag 60: 40%, seed: wheat flour 50: 50%, and powder: sucrose 60: 40%) produced granules with good flowability (Carr index ∼7.8), stable color (<i>L</i><sup>∗</sup> ≈ 80, <i>a</i><sup>∗</sup> ≈ 2.5, and <i>b</i><sup>∗</sup> ≈ 18.5), and high probiotic survival above 6.4 logCFU/g after drying, thereby meeting the functional threshold for probiotic foods. Predrying the pulp:rag mixture to 60% moisture improved the recovery yield (∼59%), reduced drying time, and enhanced bacterial viability, while the 60:40 ratio of Mixture B (flour) to sucrose offered the best balance between sweetness, color quality, and probiotic stability. Drying at 60°C was identified as the most suitable condition to preserve both product quality and viability (> 7 logCFU/g), whereas higher temperatures led to a significant decline in bacterial counts. These results demonstrate that jackfruit by-products can be effectively transformed into stable, consumer-ready probiotic granules, offering both functional health benefits and a sustainable approach to reducing food waste.</p>","PeriodicalId":15951,"journal":{"name":"Journal of Food Quality","volume":"2025 1","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jfq/5333730","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145530083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}