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Determination Methods and Influencing Factors of Grain Mechanical Properties 晶粒机械性能的测定方法和影响因素
IF 3.3 3区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-03-07 DOI: 10.1155/2024/3407485
Peng Gao, Shuangqi Tian, Xing’ao Xue, Jing Lu

Grain is extremely vulnerable to external loads during production and processing, resulting in the deterioration of grain quality. Deteriorated grain not only affects the economic value of grain but also affects the safety of storage. This has a very important relationship with the biomechanical properties of grains. It is of great significance to explore the mechanical properties of grain under different conditions and analyze the relationship between its physical and chemical properties and mechanical properties for improving its processing and eating quality. In this paper, the research methods of the mechanical properties of grains are reviewed. Various factors influencing the mechanical properties of cereals were analyzed. The relationship between the internal organizational structure of grain and its mechanical properties was discussed. This paper puts forward the shortcomings in the current research on the mechanical properties of grains and puts forward the prospect and analysis of its importance in future development in order to provide a reference for reducing crushing in the grain processing process.

谷物在生产和加工过程中极易受到外部负荷的影响,导致谷物品质下降。变质的谷物不仅会影响谷物的经济价值,还会影响储藏安全。这与谷物的生物力学特性有着非常重要的关系。探索谷物在不同条件下的力学性能,分析其物理、化学性质与力学性能之间的关系,对提高谷物的加工和食用品质具有重要意义。本文综述了谷物机械特性的研究方法。分析了影响谷物力学性能的各种因素。讨论了谷物内部组织结构与其力学性能之间的关系。本文提出了目前谷物力学性能研究中存在的不足,并对其在未来发展中的重要性进行了展望和分析,以期为减少谷物加工过程中的破碎提供参考。
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引用次数: 0
A Comprehensive Narrative Review on the Hazards of Bee Honey Adulteration and Contamination 关于蜂蜂蜜掺假和污染危害的全面叙述性综述
IF 3.3 3区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-03-07 DOI: 10.1155/2024/3512676
Ionela-Daniela Morariu, Liliana Avasilcai, Madalina Vieriu, Vasile Valeriu Lupu, Ileana Ioniuc, Branco-Adrian Morariu, Ancuța Lupu, Paula-Cristina Morariu, Oana-Lelia Pop, Vladut Mirel Burduloi, Iuliana Magdalena Starcea, Laura Trandafir

Honey bees are renowned for producing a remarkable substance known as bee honey, which stands as a functional food celebrated for its numerous health benefits. This natural wonder possesses a spectrum of advantageous properties, including anti-inflammatory, antioxidant, analgesic, antibacterial, and wound-healing qualities. However, in our modern era of heightened utilization of bee products, a new and pressing global health concern has emerged—the contamination of honey with pesticides, antibiotics, microorganisms, and heavy metals. The consumption of beekeeping products containing pesticide residues has been linked to a range of health issues, including genetic malformations, cellular degradation, allergic reactions, and even potential carcinogenic effects. Troublingly, documented cases exist of botulism in newborns resulting from the ingestion of contaminated honey. Additionally, the use of antibiotics in beekeeping practices has been associated with the concerning emergence of antibiotic resistance. This comprehensive review sheds light on the substantial consequences of honey contamination for human health. It underscores the urgent need for the establishment of a rigorous monitoring system, the validation of minimum acceptable pollutant levels, and, at the very least, the regulation of maximum residue limits for bee products, with a particular emphasis on bee honey.

蜜蜂因生产一种被称为 "蜂蜜 "的非凡物质而闻名于世,它是一种功能性食品,因其对健康的众多益处而备受赞誉。这种天然奇物具有多种优势特性,包括消炎、抗氧化、镇痛、抗菌和伤口愈合等。然而,在蜂产品利用率不断提高的当代,一个新的、紧迫的全球健康问题出现了--蜂蜜受到杀虫剂、抗生素、微生物和重金属的污染。食用含有农药残留的养蜂产品与一系列健康问题有关,包括遗传畸形、细胞退化、过敏反应,甚至潜在的致癌作用。令人不安的是,有记录显示,新生儿因摄入受污染的蜂蜜而导致肉毒杆菌中毒。此外,养蜂过程中抗生素的使用也与抗生素耐药性的出现有关。本综述揭示了蜂蜜污染对人类健康造成的严重后果。它强调迫切需要建立一个严格的监测系统,验证可接受的最低污染物水平,并至少对蜂产品的最高残留限量做出规定,其中特别强调了蜂蜂蜜。
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引用次数: 0
Characterization of Amino Acid Composition, Nutritional Value, and Taste of Fruits from Different Actinidia arguta Resources 不同放线菌资源果实的氨基酸组成、营养价值和口感特征
IF 3.3 3区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-02-29 DOI: 10.1155/2024/1005194
Yanli He, Hongyan Qin, Jinli Wen, Lin Wang, Weiyu Cao, Shutian Fan, Wenpeng Lu, Jiaqi Li, Changyu Li

The nutritional value and flavor and texture characteristics of fruits from different Actinidia argute resources were scientifically evaluated and compared. Using 35 A. arguta fruits as materials, the amino acid composition and content were determined by an automatic amino acid analyzer, and differentiation analysis, amino acid nutritional value evaluation, TAV flavor analysis, correlation analysis, PCA comprehensive evaluation, and cluster analysis were conducted to clarify the diversity of A. arguta resources in terms of amino acid content, composition, and flavor characteristics. Analysis of differential results showed that the A. arguta resource fruits contained 17 amino acids with a total amino acid content of 384.20∼2590.56 mg/100 g. The results of the nutritional value evaluation showed that the Leu of the fruits of the A. arguta resources all conformed to the ideal model proposed by FAO/WHO, and the Leu content of all the resources exceeded the human body’s needs, and it was also found that the first limiting amino acid of the Actinidia argute resources was Ile and the second limiting amino acid was Lys. TAV of the flavor-presenting amino acids was calculated to evaluate the flavor-presenting taste characteristics, and the amino acids that influenced the flavor of A. arguta fruit were Glu and Cys. PCA showed that the 2 principal components could better reflect the comprehensive information of amino acids in A. arguta, and the cumulative variance contribution rate was 87.88%, which could represent the main trend of amino acids in A. arguta. A comprehensive amino acid evaluation model was established, and the composite scores indicated that the top 5 excellent resources were S4, S10, S18, S25, and S30. Hierarchical cluster analysis classified the 35 A. arguta resources into 4 categories, which better reflected the differences in amino acid content and composition, nutritional value, and taste characteristics among A. arguta fruits from different collection sites.

科学评价和比较了不同放线菌资源果实的营养价值、风味和口感特征。以 35 个芒萁果实为材料,采用氨基酸自动分析仪测定其氨基酸组成和含量,并进行了差异分析、氨基酸营养价值评价、TAV风味分析、相关分析、PCA综合评价和聚类分析,以明确芒萁资源在氨基酸含量、组成和风味特征方面的多样性。差异分析结果表明,A. arguta 资源果实含有 17 种氨基酸,总氨基酸含量为 384.20∼2590.56 mg/100 g。营养价值评价结果表明,A. arguta 资源果实中的 Leu 均符合 FAO/WHO 提出的理想模式,所有资源果实的 Leu 含量均超过人体需要量,同时还发现,Actinidia argute 资源果实的第一限制性氨基酸为 Ile,第二限制性氨基酸为 Lys。通过计算呈味氨基酸的 TAV 值来评价呈味风味特征,结果表明影响芒果风味的氨基酸为 Glu 和 Cys。PCA结果表明,2个主成分能较好地反映文冠果氨基酸的综合信息,累积方差贡献率为87.88%,能代表文冠果氨基酸的主要变化趋势。建立了氨基酸综合评价模型,综合评分结果表明,S4、S10、S18、S25 和 S30 是前 5 位的优良资源。层次聚类分析将 35 种 A. arguta 资源分为 4 类,更好地反映了不同采集地的 A. arguta 果实在氨基酸含量和组成、营养价值和口感特征方面的差异。
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引用次数: 0
Application of Slightly Acidic Electrolyzed Water as a Potential Sanitizer in the Food Industry 微酸性电解水作为潜在消毒剂在食品工业中的应用
IF 3.3 3区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-02-29 DOI: 10.1155/2024/5559753
Abdulsudi Issa-Zacharia

The food industry has extensively explored postharvest microbial control, seeking viable technologies to ensure food safety. Although numerous chlorine-based commercial sanitizers serve this purpose, many are plagued by constraints such as instability and diminished disinfectant efficacy. These issues arise from exposure to organic matter in wash water, light, or air. As an innovative and promising alternative, slightly acidic electrolyzed water (SAEW) has emerged, captivating attention for its robust sterilization potential and eco-friendliness in agricultural and food sectors. SAEW generated via electrolysis of a diluted hydrochloric acid (HCl) solution with concentrations ranging from 2 to 6% or aqueous solution of sodium chloride (NaCl) in a nonmembrane electrolytic chamber is reported to possess equivalent antimicrobial properties as strong acidic electrolyzed water (StAEW). In contrast to traditional chlorine sanitizers, SAEW leaves less chlorine residue on sanitized foods such fresh-cut fruit and vegetables, meat, poultry, and aquatic products due to its low available chlorine concentration (ACC). Its near neutral pH of 5 to 6.5 not only renders it environmentally benign but also mitigates the production of chlorine gas, a contrast to low pH conditions seen in StAEW generation. The bactericidal effect of SAEW against various strains of foodborne pathogens is widely believed and accepted to be due to the combined action of high oxidation-reduction-potential (ORP) reactions and undissociated hypochlorite/hypochlorous acid (HOCl). Consequently, a burgeoning interest surrounds the potential of SAEW for sanitation in the food industry, offering an alternative to address shortcomings in sodium hypochlorite solutions and even StAEW. It has been hypothesized from a number of studies that SAEW treatment can increase the quality and nutritional value of harvested fruits, which in turn may enhance their ability to be stored. Therefore, SAEW is not only a promising sanitizer in the food industry but also has the potential to be an efficient strategy for encouraging the accumulation of bioactive chemicals in plants, especially if it is used extensively. This review encapsulates the latest insights concerning SAEW, encompassing its antimicrobial effectiveness, sanitization mechanism, advantages vis-à-vis other sanitizers, and plausible applications across the food industry.

食品工业对收获后微生物控制进行了广泛的探索,寻求可行的技术来确保食品安全。虽然许多氯基商用消毒剂都能达到这一目的,但许多消毒剂都存在不稳定和消毒效果下降等问题。这些问题都是由于暴露于清洗水中的有机物、光线或空气而引起的。微酸性电解水(SAEW)作为一种创新且前景广阔的替代品应运而生,因其强大的杀菌潜力以及在农业和食品领域的生态友好性而备受关注。据报道,微酸性电解水(SAEW)是在无膜电解室中通过电解浓度为 2% 至 6% 的稀盐酸(HCl)溶液或氯化钠(NaCl)水溶液产生的,具有与强酸性电解水(StAEW)同等的抗菌特性。与传统的氯消毒剂相比,由于 SAEW 的可用氯浓度(ACC)较低,因此在新鲜水果和蔬菜、肉类、家禽和水产品等消毒食品上残留的氯较少。它的 pH 值接近中性(5 至 6.5),不仅对环境无害,还能减少氯气的产生,这与 StAEW 产生过程中的低 pH 值条件形成鲜明对比。人们普遍认为,SAEW 对各种食源性病原体菌株的杀菌作用是由于高氧化还原电位(ORP)反应和未析出次氯酸盐/次氯酸(HOCl)的共同作用。因此,人们对 SAEW 在食品工业卫生方面的潜力产生了浓厚的兴趣,它为解决次氯酸钠溶液甚至 StAEW 的缺点提供了一种替代方法。许多研究推测,SAEW 处理可提高采收水果的质量和营养价值,进而增强其贮藏能力。因此,SAEW 不仅在食品工业中是一种前景广阔的消毒剂,而且还有可能成为促进植物体内生物活性化学物质积累的有效策略,尤其是在广泛使用的情况下。本综述概述了有关 SAEW 的最新见解,包括其抗菌效果、消毒机制、与其他消毒剂相比的优势以及在食品工业中的合理应用。
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引用次数: 0
Effect of Coating and Coated Paperboard Packaging on the Quality of Grapes and Apple during Storage 涂层和涂层纸板包装对贮藏期间葡萄和苹果质量的影响
IF 3.3 3区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-02-27 DOI: 10.1155/2024/9983828
Soriful Islam, Md Shakil, Md Sazzat Hossain Sarker, Md Faridunnabi Nayem, Tanjina Akter, Ishmam Haque Sachcha, Sabina Yasmin

Edible coatings and active packaging have become more prevalent in response to changing consumption patterns and market trends to enhance the quality and safety of fresh products. In this work, we investigated the effect of aloe vera gel (AVG) coating and paraffin wax-coated paperboard (PWB) packaging on the postharvest quality attributes of both grapes and apples during storage. The fruits were coated with 50% AVG concentrations, and the inner wall of the corrugated paperboard was coated with paraffin wax emulsion. The grapes and apples were stored for 12 and 35 days, respectively, at ambient conditions (25 ± 3°C and 80–85% relative humidity). The physicochemical properties, microbiological attributes, and decay incidence of the fruits were analyzed at intervals during storage. Both fruits treated with AVG and PWB packaging retained better qualities than the control at the final day of the storage period. Particularly, PWB packaging provided considerably superior quality from the control sample in terms of weight loss (≈54% and 32%), firmness (≈48% and 68%), and color difference (≈30% and 28%) for both grapes and apples. These findings would introduce a novel approach for preserving the quality attributes of both climacteric and nonclimacteric fruits for a prolonged storage period at ambient temperature by PWB packaging and AVG coating.

为了应对不断变化的消费模式和市场趋势,提高新鲜产品的质量和安全性,食用涂层和活性包装变得越来越普遍。在这项工作中,我们研究了芦荟胶(AVG)涂层和石蜡涂层纸板(PWB)包装对葡萄和苹果贮藏期间采后质量属性的影响。水果涂上浓度为 50%的 AVG,瓦楞纸板内壁涂上石蜡乳液。葡萄和苹果在环境条件(25 ± 3°C 和 80-85% 的相对湿度)下分别贮藏了 12 天和 35 天。在贮藏期间,每隔一段时间对水果的理化特性、微生物属性和腐烂率进行分析。在贮藏期的最后一天,经 AVG 和 PWB 包装处理的水果都比对照组保持了更好的品质。特别是在葡萄和苹果的重量损失(≈54% 和 32%)、硬度(≈48% 和 68%)和色差(≈30% 和 28%)方面,PWB 包装都比对照样品的质量好得多。这些发现为通过 PWB 包装和 AVG 涂层在环境温度下长期贮藏气候性水果和非气候性水果的质量特性提供了一种新方法。
{"title":"Effect of Coating and Coated Paperboard Packaging on the Quality of Grapes and Apple during Storage","authors":"Soriful Islam,&nbsp;Md Shakil,&nbsp;Md Sazzat Hossain Sarker,&nbsp;Md Faridunnabi Nayem,&nbsp;Tanjina Akter,&nbsp;Ishmam Haque Sachcha,&nbsp;Sabina Yasmin","doi":"10.1155/2024/9983828","DOIUrl":"10.1155/2024/9983828","url":null,"abstract":"<p>Edible coatings and active packaging have become more prevalent in response to changing consumption patterns and market trends to enhance the quality and safety of fresh products. In this work, we investigated the effect of aloe vera gel (AVG) coating and paraffin wax-coated paperboard (PWB) packaging on the postharvest quality attributes of both grapes and apples during storage. The fruits were coated with 50% AVG concentrations, and the inner wall of the corrugated paperboard was coated with paraffin wax emulsion. The grapes and apples were stored for 12 and 35 days, respectively, at ambient conditions (25 ± 3°C and 80–85% relative humidity). The physicochemical properties, microbiological attributes, and decay incidence of the fruits were analyzed at intervals during storage. Both fruits treated with AVG and PWB packaging retained better qualities than the control at the final day of the storage period. Particularly, PWB packaging provided considerably superior quality from the control sample in terms of weight loss (≈54% and 32%), firmness (≈48% and 68%), and color difference (≈30% and 28%) for both grapes and apples. These findings would introduce a novel approach for preserving the quality attributes of both climacteric and nonclimacteric fruits for a prolonged storage period at ambient temperature by PWB packaging and AVG coating.</p>","PeriodicalId":15951,"journal":{"name":"Journal of Food Quality","volume":"2024 1","pages":""},"PeriodicalIF":3.3,"publicationDate":"2024-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139980750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Changes in Techno-Functional Characteristics of Cow Colostrum Powder Prepared by Freeze Drying 冷冻干燥法制备的牛初乳粉技术功能特性的变化
IF 3.3 3区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-02-15 DOI: 10.1155/2024/2608059
Anamika Das, Raman Seth, Ayon Tarafdar, Swarnima Dey, Yogesh Kumar, Ranjna Sirohi

The present study investigates the characteristics of freeze-dried bovine colostrum powder prepared from the first three milkings of Karan fries cattle at different intervals. Bioactive components of bovine colostrum are heat sensitive, and therefore, in order to retain the biological activity of different components, it should be subjected to minimal nonthermal treatment. In this study, the optimum temperature/time combination of 60°C/45 min was used for the processing of cow colostrum. At this temperature, the microbial count significantly decreased as compared to raw colostrum. In freeze-dried bovine skimmed colostrum powder (BSCP) and bovine colostrum whey powder (BCWP), IgG emerged as the highest fraction among immune factors, whereas in growth factors, insulin-like growth factor 1(IGF1) and transforming growth factor β2 (TGFβ2) were found in large proportion as compared to insulin-like growth factor 2 (IGF2) and transforming growth factor β1 (TGFβ1). The IgG content (per 100 g) of BSCP prepared from 1st, 2nd, and 3rd milking was found to be 36.62, 27.87, and 20.51 g, respectively, whereas IgG content (per 100 g) of BCWP prepared from 1st, 2nd, and 3rd milking was found to be 33.32, 24.53, and 16.81 g, respectively. The IGF1 content (per 100 g) of BSCP prepared from 1st, 2nd, and 3rd milking was found to be 921.6, 741.2, and 617.2 μg, respectively, whereas IGF1 content (per 100 g) of BCWP prepared from 1st, 2nd, and 3rd milking was found to be 869.8, 688, and 454.4 μg, respectively. The TGFβ2 content (per 100 g) of BSCP prepared from 1st, 2nd, and 3rd milking was found to be 1278, 1076, and 856.8 μg, respectively, whereas TGFβ2 content (per 100 g) of BCWP prepared from 1st, 2nd, and 3rd milking was found to be 1167, 950.2, and 769.2 μg, respectively. The microstructure of freeze-dried cow colostrum whey powder revealed that its pore size was more than that of skimmed colostrum powder. BCWP exhibited lower phagocytic activity as well as lymphocyte proliferation index as compared to BSCP which was carried out in vitro. The observations of the study provide an insight about the components present in BCWP and BSCP apart from the physical characteristics of the products which might pave the way for its utilization in various food formats.

本研究调查了冷冻干燥牛初乳粉的特性,这些牛初乳粉是从卡兰油牛的前三次挤奶中以不同的间隔时间制成的。牛初乳中的生物活性成分对热敏感,因此,为了保留不同成分的生物活性,应该对其进行最低限度的非热处理。本研究采用 60°C/45 分钟的最佳温度/时间组合来处理牛初乳。与未加工的牛初乳相比,该温度下的微生物数量明显减少。在冻干牛脱脂牛初乳粉(BSCP)和牛初乳乳清粉(BCWP)中,IgG是免疫因子中含量最高的部分,而在生长因子中,胰岛素样生长因子1(IGF1)和转化生长因子β2(TGFβ2)的比例要高于胰岛素样生长因子2(IGF2)和转化生长因子β1(TGFβ1)。第 1 次、第 2 次和第 3 次挤奶制备的 BSCP 的 IgG 含量(每 100 克)分别为 36.62 克、27.87 克和 20.51 克,而第 1 次、第 2 次和第 3 次挤奶制备的 BCWP 的 IgG 含量(每 100 克)分别为 33.32 克、24.53 克和 16.81 克。第 1 次、第 2 次和第 3 次挤奶制备的 BSCP 的 IGF1 含量(每 100 克)分别为 921.6、741.2 和 617.2 μg,而第 1 次、第 2 次和第 3 次挤奶制备的 BCWP 的 IGF1 含量(每 100 克)分别为 869.8、688 和 454.4 μg。第 1 次、第 2 次和第 3 次挤奶制备的 BSCP 的 TGFβ2 含量(每 100 克)分别为 1278、1076 和 856.8 μg,而第 1 次、第 2 次和第 3 次挤奶制备的 BCWP 的 TGFβ2 含量(每 100 克)分别为 1167、950.2 和 769.2 μg。冻干牛初乳乳清粉的微观结构显示,其孔径大于脱脂牛初乳粉。在体外实验中,与 BSCP 相比,BCWP 的吞噬活性和淋巴细胞增殖指数较低。除了产品的物理特性外,本研究的观察结果还提供了有关 BCWP 和 BSCP 中存在的成分的见解,这可能会为其在各种食品形式中的应用铺平道路。
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引用次数: 0
Modified Gegen Qinlian Decoction Ameliorates DSS-Induced Ulcerative Colitis in Mice by Inhibiting Ferroptosis via Nrf2/GPX4 Pathway 改良葛根秦连煎剂通过 Nrf2/GPX4 通路抑制铁凋亡,从而改善 DSS 诱导的小鼠溃疡性结肠炎
IF 3.3 3区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-02-07 DOI: 10.1155/2024/6802701
Jinke Huang, Jiaqi Zhang, Zhihong Liu, Jing Ma, Fengyun Wang, Xudong Tang

Objective. Ferroptosis, a form of programmed cell death, is considered a novel target for the treatment of ulcerative colitis (UC). The aim of this study was to explore whether modified Gegen Qinlian decoction (MGQD) ameliorates UC in mice via mediating ferroptosis. Methods. Mice with dextran sulfate sodium- (DSS-) induced colitis were administered with MGQD for seven days. Subsequently, iron, malondialdehyde (MDA), glutathione (GSH), and reactive oxygen species (ROS) were measured. ELISA and immunohistochemistry were used to evaluate the levels of proinflammatory cytokines and tight junction proteins, respectively. Transmission electron microscopy was used to reveal mitochondrial morphology. Western blot and qRT-PCR analyses were used to assess the expression levels of the proteins of Nrf2/GPX4 pathway. The docking affinity of MGQD and Nrf2 was assessed using AutoDock Vina 1.1.2. Results. Ferroptosis was identified in mice with UC, as demonstrated by mitochondrial disruption, MDA and ROS production, iron overload, decrease in GSH level, and abnormal expression of core marker proteins of ferroptosis. After MGQD treatment, these characteristic changes of ferroptosis were significantly reversed, along with concomitant activation of the Nrf2/GPX4 pathway. Furthermore, molecular docking analysis revealed that MGQD had a high affinity for Nrf2. Conclusion. MGQD may ameliorate UC by inhibiting ferroptosis via the activation of Nrf2/GPX4 pathway. This study provided new insights into the application of MGQD complementary therapy for UC.

目的。铁凋亡是一种程序性细胞死亡,被认为是治疗溃疡性结肠炎(UC)的新靶点。本研究旨在探讨改良格根秦连煎(MGQD)是否能通过介导铁突变来改善小鼠的溃疡性结肠炎。研究方法给右旋糖酐硫酸钠(DSS)诱导的结肠炎小鼠服用改良型格根秦连煎(MGQD)七天。随后测量铁、丙二醛(MDA)、谷胱甘肽(GSH)和活性氧(ROS)。酶联免疫吸附试验和免疫组织化学分别用于评估促炎细胞因子和紧密连接蛋白的水平。透射电子显微镜用于显示线粒体形态。Western 印迹和 qRT-PCR 分析用于评估 Nrf2/GPX4 通路蛋白的表达水平。使用 AutoDock Vina 1.1.2 评估了 MGQD 与 Nrf2 的对接亲和力。结果在UC小鼠中发现了铁变态反应,表现为线粒体破坏、MDA和ROS产生、铁超载、GSH水平下降以及铁变态反应核心标志蛋白的异常表达。经MGQD治疗后,这些铁变态反应的特征性变化明显逆转,同时Nrf2/GPX4通路也被激活。此外,分子对接分析表明,MGQD 与 Nrf2 有很高的亲和力。结论MGQD可通过激活Nrf2/GPX4通路抑制铁变态反应,从而改善尿毒症。这项研究为MGQD辅助治疗UC的应用提供了新的见解。
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引用次数: 0
Application of Near-Infrared Spectroscopy to Rapidly Classify the Chinese Quince Fruits from Different Habitats 应用近红外光谱对不同生境的榠楂进行快速分类
IF 3.3 3区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-02-07 DOI: 10.1155/2024/6217243
Songfeng Diao, Xiaoqian Tang, Lin Huang, Yanjie Li, Xiongfei Fan, Wenhao Shao

The ecological habitats of Chinese quince (Chaenomeles speciosa Nakai) fruits affect their phenotype. Currently, limited or no rapid method exists for classifying Chinese quince fruit from different ecosystems. This study developed a partial least squares discriminant analysis (PLS-DA) classification model to effectively and nondestructively classify 663 Chinese quince fruit samples from six environments in 2020. PLS-DA models and other variable selection approaches were used in this study. The near-infrared spectroscopy (NIRs) absorption spectra of raw Chinese quince fruit samples from six habitats showed a similar shape. The spectra of each environment showed little variance. The raw fruit spectra varied significantly among habitat categories after the first derivative preprocessing phase. The uninformative variable elimination (UVE) variable selection approach had greater calibration and validation set specificity of 0.93 and 0.98. This study found the best classification specificity using the UVE variable selection approach compared to other methods including the PLS-DA model without variable selection. The UVE approach improved Yunnan habitat categorization specificity from 86% to 88% when integrated with PLS-DA. Additionally, the validation set for quinces originating from Anhui, Chongqing, Hubei, Shandong, and Zhejiang achieved an ideal classification score of 100%. The findings of the study indicated that PLS-DA can serve as an alternative approach for classifying the habitats of Chinese quince fruits. When used in conjunction with other methods, this technique can assist researchers, scientists, and industry professionals in identifying the main factors responsible for significant variations in the habitats, composition, and quality of Chinese quince fruits.

中国榅桲(Chaenomeles speciosa Nakai)果实的生态栖息地会影响其表型。目前,对来自不同生态系统的榠楂果实进行分类的快速方法非常有限,甚至没有。本研究建立了偏最小二乘判别分析(PLS-DA)分类模型,对来自 2020 年六种环境的 663 个榠楂果实样本进行了有效的非破坏性分类。本研究采用了 PLS-DA 模型和其他变量选择方法。来自六种生境的榠楂生果样品的近红外光谱(NIRs)吸收光谱显示出相似的形状。各环境的光谱差异很小。在第一导数预处理阶段后,不同生境类别的生果光谱差异很大。无信息变量消除(UVE)变量选择方法的校准和验证集特异性更高,分别为 0.93 和 0.98。与其他方法(包括未进行变量选择的 PLS-DA 模型)相比,本研究发现使用 UVE 变量选择方法的分类特异性最好。将 UVE 方法与 PLS-DA 结合使用时,云南栖息地分类特异性从 86% 提高到了 88%。此外,原产于安徽、重庆、湖北、山东和浙江的榅桲的验证集达到了 100%的理想分类得分。研究结果表明,PLS-DA 可以作为中国榅桲果实生境分类的替代方法。当与其他方法结合使用时,该技术可帮助研究人员、科学家和行业专业人员确定造成中国榅桲果实生境、成分和质量显著变化的主要因素。
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引用次数: 0
BLSENet: A Novel Lightweight Bilinear Convolutional Neural Network Based on Attention Mechanism and Feature Fusion Strategy for Apple Leaf Disease Classification BLSENet:基于注意机制和特征融合策略的新型轻量级双线性卷积神经网络,用于苹果叶病分类
IF 3.3 3区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-02-06 DOI: 10.1155/2024/5561625
Tianyu Fang, Jialin Zhang, Dawei Qi, Mingyu Gao

Accurate identification of apple leaf diseases is of great significance for improving apple yield. The lesion area of the apple leaf disease image is small and vulnerable to background interference, which easily leads to low recognition accuracy. To solve this problem, a lightweight bilinear convolutional neural network (CNN) model named BLSENet based on attention mechanism is designed. The model consists of two subnetworks, and each subnetwork is embedded with a Squeeze-and-Excitation (SE) module. By using the feature extraction ability of the two subnetworks and combining the bilinear feature CONCAT operation, the multiscale features of the image are obtained. Compared with the unimproved model LeNet-5 (84.63%), BLSENet has higher accuracy in the test set, which indicates that SE module and bilinear feature fusion have a positive effect on the performance of the model, and BLSENet has the ability to identify apple leaf diseases. The model has achieved the expected goal and can provide technical support for accurate identification and real-time monitoring of apple disease images.

准确识别苹果叶病对提高苹果产量意义重大。苹果叶病图像的病斑面积小,易受背景干扰,容易导致识别准确率低。为解决这一问题,本文设计了一种基于注意力机制的轻量级双线性卷积神经网络(CNN)模型,命名为 BLSENet。该模型由两个子网络组成,每个子网络都嵌入了一个挤压激励(SE)模块。利用两个子网络的特征提取能力,结合双线性特征 CONCAT 运算,得到图像的多尺度特征。与未经改进的 LeNet-5 模型(84.63%)相比,BLSENet 在测试集中的准确率更高,这表明 SE 模块和双线性特征融合对模型的性能有积极影响,BLSENet 已具备识别苹果叶病的能力。该模型达到了预期目标,可为苹果病害图像的准确识别和实时监测提供技术支持。
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引用次数: 0
Determination of 60 Food-Borne Stimulant Drug Residues in Animal-Derived Foods by Solid-Phase Extraction Purification and Ultra-High-Performance Liquid Chromatography-Quadrupole/Orbitrap High-Resolution Mass Spectrometry 固相萃取净化和超高效液相色谱-四极杆/轨道阱高分辨质谱法测定动物源性食品中的 60 种食源性兴奋剂残留量
IF 3.3 3区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-02-01 DOI: 10.1155/2024/3859819
Liangna He, Qiang Li, Junmei Ma, Meirong Cao, Lixin Fan, Xiaowei Ren, Guohua Shi, Yan Zhang

A method for the rapid screening and confirmation of 60 food-borne stimulants in animal-derived foods was developed using the ultra-high-performance liquid chromatography-quadrupole/orbitrap high-resolution mass spectrometry (UPLC-Q/Orbitrap HRMS). After enzymatic hydrolysis at 37°C for 16 h, the samples were extracted with acetonitrile solution. The extraction solution was purified by PRiME HLB pass-through solid-phase extraction cartridge and redissolved by nitrogen blowing. The target compounds of reconstitution fluid were separated by Hypersil GOLD™ VANQUISH column (2.1 mm × 100 mm, 1.9 μm). The raw data were collected for the target compounds in Full Scan-ddMS2 mode, and then the Quan Browser module under Xcalibur software can be used to analyze the quantitative results of the samples. The combination of Tracefinder and mzVault software can realize the qualitative screening and confirmation of the samples. The results showed that the relative deviation of the exact mass of the 60 food-borne stimulants was less than 5.0 × 10−6, which had a good linear relationship in the corresponding concentration, and the correlation coefficient (R2) was greater than 0.9966. The detection limit ranged from 0.05 to 0.5 μg/kg. The quantification limit ranged from 0.1 to 1 μg/kg. The method recoveries ranged from 70.2% to 111.9% with relative standard deviations of 0.05% to 9.00% (n = 6). The method is easy to operate, covers a wide range of targets, and has high efficiency and accuracy. It is suitable for rapid screening and confirmation of various food-derived stimulants in animal-derived foods and provides assistance for food safety in the major events.

利用超高效液相色谱-四极杆/比特阱高分辨质谱(UPLC-Q/Orbitrap HRMS)建立了一种快速筛查和确认动物源性食品中60种食源性兴奋剂的方法。样品在 37°C 下酶水解 16 小时后,用乙腈溶液提取。萃取液经 PRiME HLB 直通式固相萃取柱净化,氮吹重新溶解。重组液中的目标化合物经 Hypersil GOLD™ VANQUISH 色谱柱(2.1 mm × 100 mm, 1.9 μm)分离。在 Full Scan-ddMS2 模式下采集目标化合物的原始数据,然后使用 Xcalibur 软件下的 Quan Browser 模块分析样品的定量结果。结合 Tracefinder 和 mzVault 软件可实现对样品的定性筛选和确认。结果表明,60 种食源性兴奋剂的准确质量相对偏差小于 5.0 × 10-6,与相应的浓度呈良好的线性关系,相关系数()大于 0.9966。检出限为 0.05 至 0.5 μg/kg。定量限为 0.1 至 1 μg/kg。方法的回收率为 70.2% 至 111.9%,相对标准偏差为 0.05% 至 9.00%(n = 6)。该方法操作简便、适用目标物范围广、高效准确。该方法适用于快速筛查和确认动物源性食品中的各种食品源兴奋剂,为重大活动中的食品安全提供帮助。
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引用次数: 0
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Journal of Food Quality
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