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Correction to “Comparison of DNA Extraction Methods for the Detection of Canned Tuna Species with DNA Mini-Barcoding” 更正“DNA微型条形码检测金枪鱼罐头品种的DNA提取方法比较”
IF 2.9 3区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2025-09-26 DOI: 10.1155/jfq/9828214

A. J. Emmi, B. Fatusin, and R. S. Hellberg, “Comparison of DNA Extraction Methods for the Detection of Canned Tuna Species with DNA Mini-Barcoding,” Journal of Food Quality 2023 (2023): 7121260, https://doi.org/10.1155/2023/7121260.

In the article, there are errors in Section 2.3:

“The reaction mixture for canned tuna fish samples included the following: 11.2 mL sterile H2O, 17.5 mL Qiagen HotStarTaq Plus Master Mix (2X), 0.7 mL each of one 10-μM forward primer (CR_F) and two 10-μM reverse primers (CR_R1 and CR_R2) (Mitchell and Hellberg, 2016), and 4.2 mL of DNA template, for a total reaction volume of 35 μL.”

Should read:

“The reaction mixture for canned tuna fish samples included the following: 11.2 μL sterile H2O, 17.5 μL Qiagen HotStarTaq Plus Master Mix (2X), 0.7 μL each of one 10-μM forward primer (CR_F) and two 10-μM reverse primers (CR_R1 and CR_R2) (Mitchell and Hellberg, 2016), and 4.2 μL of DNA template, for a total reaction volume of 35 μL.”

“Precast 2.0% E-Gels with ethidium bromide (Invitrogen, Carlsbad, CA, USA) were used to confirm DNA amplification, with 20 mL PCR product loaded into each sample lane.”

Should read:

“Precast 2.0% E-Gels with ethidium bromide (Invitrogen, Carlsbad, CA, USA) were used to confirm DNA amplification, with 20 μL PCR product loaded into each sample lane.”

Lastly, there are errors in Section 3.4:

“Legally, the term “light tuna” applies to tuna with a Munsell value not greater than 5.3, while the term “white tuna” is reserved specifically for albacore and must not exceed a Munsell value of 6.3 (21 CFR 161.190 canned tuna). The presence of albacore tuna in a sample labeled as “light tuna” was also reported by Mitchell and Hellberg [6]. Both instances of mislabeling identified in the current study could cause potential harm to at-risk groups, such as women and children, because of differences in mercury levels between tuna species.”

Should read:

“Section 3.4: Legally, the term “light tuna” applies to tuna with a Munsell value not darker than 5.3, while the term “white tuna” is reserved specifically for albacore and must not be darker than a Munsell value of 6.3 (21 CFR 161.190 canned tuna). The presence of albacore tuna in a sample labeled as “light tuna” was also reported by Mitchell and Hellberg [6]. Both labeling concerns identified in the current study could cause potential harm to at-risk groups, such as women and children, because of differences in mercury levels between tuna species.”

We apologize for these errors.

A. J. Emmi, B. Fatusin和R. S. Hellberg,“DNA提取方法与DNA迷你条形码检测罐装金枪鱼物种的比较”,《食品质量杂志》2023 (2023):7121260,https://doi.org/10.1155/2023/7121260.In文章中,2.3节有错误:“罐装金枪鱼样品的反应混合物包括以下内容:11.2 mL的灭菌水,17.5 mL Qiagen HotStarTaq Plus Master Mix (2X), 1个10-μM正引物(CR_F)和2个10-μM反向引物(CR_R1和CR_R2)各0.7 mL (Mitchell and Hellberg, 2016), 4.2 mL DNA模板,总反应体积为35 μL。应注意:金枪鱼罐头样品的反应混合物包括:11.2 μL无菌H2O, 17.5 μL Qiagen HotStarTaq Plus Master Mix (2X), 1个10 μ m正向引物(CR_F)和2个10 μ m反向引物(CR_R1和CR_R2)各0.7 μL (Mitchell and Hellberg, 2016), 4.2 μL DNA模板,总反应体积为35 μL。“使用含有溴化乙啶的预制2.0% e -凝胶(Invitrogen, Carlsbad, CA, USA)确认DNA扩增,每个样品通道装入20ml PCR产物。”应读:“使用含有溴化乙啶的预制2.0% e -凝胶(Invitrogen, Carlsbad, CA, USA)确认DNA扩增,每个样品通道装入20 μL PCR产物。”最后,在第3.4节中有错误:“法律上,术语“轻金枪鱼”适用于孟塞尔值不大于5.3的金枪鱼,而术语“白金枪鱼”专门用于长鳍金枪鱼,不得超过孟塞尔值6.3 (21 CFR 161.190罐装金枪鱼)。在标注为“轻金枪鱼”的样本中也发现了长鳍金枪鱼,这也是米切尔和海尔伯格的报道。目前研究中发现的这两种标签错误都可能对妇女和儿童等高危人群造成潜在伤害,因为不同金枪鱼种类的汞含量不同。”应阅读:“第3.4节:法律上,术语“浅金枪鱼”适用于蒙塞尔值不高于5.3的金枪鱼,而术语“白金枪鱼”专门用于长鳍金枪鱼,不得高于蒙塞尔值6.3 (21 CFR 161.190罐装金枪鱼)。在标注为“轻金枪鱼”的样本中也发现了长鳍金枪鱼,这也是米切尔和海尔伯格的报道。目前研究中发现的两种标签问题都可能对妇女和儿童等高危人群造成潜在伤害,因为不同金枪鱼种类的汞含量存在差异。”我们为这些错误道歉。
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引用次数: 0
Antimicrobial Resistance, Biofilms, and Toxin Profiles of Methicillin-Resistant Staphylococcus aureus in the Beef Value Chain of Bangladesh 孟加拉国牛肉价值链中耐甲氧西林金黄色葡萄球菌的抗菌素耐药性、生物膜和毒素谱
IF 2.9 3区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2025-09-24 DOI: 10.1155/jfq/7401437
Fahmida Jahan Fahim, Sohel Rana, Rimi Das, Mithu Chandra Roy, Md Mahmudul Hasan, Md Sodor Uddin, Nadia Sultana, Kazi Zinnah, Kulsum Begum Chowdhury, Eman Zahran, Monira Noor, Ferdaus Mohd Altaf Hossain

Methicillin-resistant Staphylococcus aureus (MRSA), responsible for various foodborne illnesses, presents substantial hurdles throughout the beef value chain due to its ability to form robust biofilms and produce different types of superantigenic toxins. This study aimed to characterize biofilm-producing S. aureus isolated from raw beef and ready-to-eat (RTE) food samples collected from Gazipur and Dhaka City Corporations. A total of 240 samples were collected, with 120 from each city corporation, comprising 60 raw beef and 60 RTE samples. These samples were examined for colony forming unit (CFU) to assess the safety margin limit, as well as undergo screening for MRSA presence, antibiotic sensitivity testing through the Kirby–Bauer disc diffusion method, toxin profiling, and the characterization of biofilm-forming genes (icaA, icaB, icaC, and icaD). Finally, we performed a phylogenetic analysis to study the evolutionary relationship among strains. Results revealed the presence of S. aureus (nuc gene) in 167 out of 240 samples, with CFU counts surpassing safety thresholds within the One Health interfaces suggested by the Bangladesh Food Safety Authority (maximum limit for raw meat 103 cfu/g and RTE 102 cfu/g). MRSA was detected in 40.83% of the total samples, all exhibiting resistance to several antibiotics. Positive isolates from both municipal corporations were completely (100%) resistant to oxacillin, ampicillin, and amoxicillin, yet demonstrated greater susceptibility to vancomycin (88.6%) and linezolid (34.1%). Additionally, Staphylococcal enterotoxin SEa was present in 45.79% of samples, followed by SEd (24.39%) and SEb (20.57%), while TSST-1 was present in only 2.81% of total samples. Again, it was observed that most of the isolates have biofilm formation capacity and harbored icaA (65%), icaD (61%), icaC (50%), and icaB (48%) biofilm-forming genes. The outcome of phylogenetic analysis revealed that our isolates have a close relationship with the isolates of blood samples from the United States. The findings highlight the critical need for enhanced food safety measures. Therefore, surveillance of MRSA in the beef value chain and the implementation of stringent hygiene practices are imperative to mitigate the risk of colonization and spread of MRSA strains that produce biofilms.

耐甲氧西林金黄色葡萄球菌(MRSA)是导致各种食源性疾病的原因,由于其能够形成坚固的生物膜并产生不同类型的超抗原毒素,因此在整个牛肉价值链中存在重大障碍。本研究旨在鉴定从加济普尔和达卡市公司收集的生牛肉和即食食品样本中分离的产生生物膜的金黄色葡萄球菌。共收集了240份样本,其中120份来自每个城市公司,包括60份生牛肉和60份RTE样本。对这些样品进行菌落形成单位(CFU)检查以评估安全边际限制,并进行MRSA存在筛选,通过Kirby-Bauer圆盘扩散法进行抗生素敏感性测试,毒素谱分析和生物膜形成基因(icaA, icaB, icaC和icaD)的表征。最后,我们进行了系统发育分析,以研究菌株之间的进化关系。结果显示,240份样品中有167份存在金黄色葡萄球菌(nuc基因),CFU计数超过了孟加拉国食品安全局建议的One Health接口的安全阈值(生肉的最大限值为103 CFU /g, RTE的最大限值为102 CFU /g)。40.83%的样品检出MRSA,均表现出对几种抗生素的耐药。来自两家市政公司的阳性分离株对氧苄西林、氨苄西林和阿莫西林完全(100%)耐药,但对万古霉素(88.6%)和利奈唑胺(34.1%)更敏感。此外,45.79%的样品中存在葡萄球菌肠毒素SEa,其次是SEd(24.39%)和SEb(20.57%),而TSST-1仅占总样品的2.81%。再次观察到,大多数分离株具有生物膜形成能力,并携带icaA (65%), icaD (61%), icaC(50%)和icaB(48%)生物膜形成基因。系统发育分析结果表明,我们的分离株与美国的血液样本分离株有密切的关系。研究结果强调了加强食品安全措施的迫切需要。因此,对牛肉价值链中的MRSA进行监测和实施严格的卫生措施对于降低产生生物膜的MRSA菌株定植和传播的风险至关重要。
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引用次数: 0
A Realist Review of the Effectiveness of Sustainable Food Packaging Interventions in Food Waste and Healthy Eating 可持续食品包装干预食品浪费和健康饮食有效性的现实主义回顾
IF 2.9 3区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2025-09-24 DOI: 10.1155/jfq/1504945
Tian Zeng

The significant environmental and health impacts of food packaging underscore the need for more effective interventions to promote sustainable lifestyles and consumer well-being. This study conducted a realist review of 96 articles published in top-tier journals between 2019 and 2024, focusing on packaging interventions aimed at promoting healthy food consumption and food waste reduction. It explored the mechanisms and contextual factors that influence the effectiveness of such interventions in real-world settings. Using directed content analysis guided by a context–mechanism–outcome (CMO) framework, the study identified how, for whom, under what circumstances, and why sustainable food packaging interventions succeed. The findings yielded several guiding principles to enhance intervention effectiveness, including: (1) improving clarity of packaging communication to help consumers assess food healthiness; (2) involving consumers in packaging innovation to increase acceptance of novel technologies; and (3) developing business models that share responsibility, benefits, and risks across the food supply chain. This research contributes to the literature by (1) offering deeper insights into the contextual and mechanistic pathways that shape outcomes of sustainable food packaging interventions and (2) proposing 12 actionable principles to support healthier eating and food waste reduction for key stakeholders.

食品包装对环境和健康的重大影响突出表明,需要采取更有效的干预措施,促进可持续的生活方式和消费者福祉。本研究对2019年至2024年间发表在顶级期刊上的96篇文章进行了现实主义回顾,重点关注旨在促进健康食品消费和减少食物浪费的包装干预措施。它探讨了在现实环境中影响此类干预措施有效性的机制和背景因素。使用由上下文-机制-结果(CMO)框架指导的直接内容分析,该研究确定了如何,为谁,在什么情况下,以及为什么可持续食品包装干预成功。研究结果提出了提高干预效果的几项指导原则,包括:(1)提高包装宣传的清晰度,以帮助消费者评估食品健康;(2)让消费者参与包装创新,提高对新技术的接受度;(3)发展在整个食品供应链中分担责任、利益和风险的商业模式。本研究对文献的贡献在于:(1)对形成可持续食品包装干预结果的背景和机制途径提供了更深入的见解;(2)为关键利益相关者提出了12项可操作的原则,以支持更健康的饮食和减少食物浪费。
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引用次数: 0
Impact of Mandarin Peel and Pulp on the Physicochemical, Rheological, and Sensory Characteristics of Low-Fat Ice Cream 柑桔皮和果肉对低脂冰淇淋理化、流变学和感官特性的影响
IF 2.9 3区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2025-09-17 DOI: 10.1155/jfq/4623486
Hind Abdalluh Abu-Hiamed, Rafik Abd El-Rahman Mohammed Khalil, Wafa Daham Almarkhan, Sayed Mohamed Mokhtar

Consumer demand for low-fat products has increased due to their perceived association with reduced risks of obesity, cardiovascular disease, and certain cancers. Mandarin peel (MPE) and mandarin pulp (MPU) are rich sources of bioactive compounds, including dietary fibers, total phenolic content (TPC), antioxidant activity (AA), and vitamin C that may help mitigate disease risks associated with high-fat diets. The effect of using 4% and 7% MPE or 10% and 15% MPU in the manufacture of low-fat ice cream (LFIC) on its quality properties was investigated. Full-fat ice cream (FFIC; 6% fat) and LFIC (1% fat) were used as controls for comparison. The low-fat mixes enriched with MPE significantly exhibited higher specific gravity (SG), rheological characteristics, overrun, TPC, and AA than LFIC control. Also, adding MPU increased freezing point depression, TPC, and AA. Using of MPE in LFIC significantly (p < 0.05) decreased the melting rate as compared to the LFIC control or MPU-treated ice cream samples. Sensory analysis showed that MPE-treated (4% and 7%) samples had higher overall acceptability scores throughout 30 days of frozen storage than LFIC and ranked a similar overall acceptability of a full fat control with decreasing production costs by 17.74% and 17.53%, respectively, while using MPU enhanced flavor intensity with slight improvement for body and texture. The results of the present study valorize exploiting of MPE up to 7% in developing a novel LFIC with strong AA, health benefits, and desirable sensory properties.

消费者对低脂产品的需求有所增加,因为人们认为它们与降低肥胖、心血管疾病和某些癌症的风险有关。橘子皮(MPE)和橘子果肉(MPU)是生物活性化合物的丰富来源,包括膳食纤维、总酚含量(TPC)、抗氧化活性(AA)和维生素C,可能有助于减轻与高脂肪饮食相关的疾病风险。研究了低脂冰淇淋(LFIC)中添加4%和7% MPE或10%和15% MPU对其品质性能的影响。全脂冰淇淋(FFIC; 6%脂肪)和LFIC(1%脂肪)作为对照进行比较。与LFIC对照相比,富含MPE的低脂混合物具有更高的比重(SG)、流变特性、溢出、TPC和AA。同时,添加MPU可提高凝固点降、TPC和AA。与LFIC对照或mpu处理的冰淇淋样品相比,在LFIC中使用MPE显著降低了融化速度(p < 0.05)。感官分析表明,与LFIC相比,mpe处理(4%和7%)的样品在30天的冷冻储存过程中具有更高的总体可接受性得分,并且与全脂对照的总体可接受性相似,生产成本分别降低了17.74%和17.53%,而MPU则增强了风味强度,并略微改善了口感和质地。本研究的结果表明,在开发一种具有强AA、健康益处和理想感官特性的新型LFIC时,MPE的利用率可达7%。
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引用次数: 0
Prevalence and Antibiotic Resistance of Selected Foodborne Pathogens in Beef Sold Within Kumasi Metropolis, Ghana 在加纳库马西市出售的牛肉中选定食源性病原体的患病率和抗生素耐药性
IF 2.9 3区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2025-09-09 DOI: 10.1155/jfq/2991675
Patrick Amponsah Mensah, Daniel Adjei, Janice Ewurabena Williams, Grace Manu Arhin, Derrick Baffour Asare, Osei Frimpomaa Saviour, Josephine Adufa Manu, Emmanuel Fiifi Idun, Irene Kookailey Afutu, Richard Kwamena Abbiw, Sandra Abankwah-Kwarteng, Kwadwo Boampong

Foodborne pathogens pose significant food safety risks and understanding their presence and resistance patterns is crucial for public health interventions. This study assessed the prevalence and antibiotic resistance profiles of selected foodborne pathogens in raw beef samples from Kumasi Abattoir and Kejetia Market in the Kumasi Metropolis in Ghana. Beef samples were analyzed for Bacillus cereus, Listeria monocytogenes, and Escherichia coli. Antibiotic susceptibility testing was conducted to determine resistance profiles. The study revealed the varying prevalence of the pathogens, with B. cereus detected in 27.55% of the samples, L. monocytogenes in 55.41%, and Escherichia coli in 59.91%, respectively. Approximately 76% of all samples had at least one of the three bacteria, with microbial loads above bacteria-specific acceptable standards. Most of the E. coli strains were positive for ESBL, while all B. cereus and L. monocytogenes strains were multidrug resistant with multiple antibiotic-resistant indices of more than 0.2. Nine and four AMR patterns emerged in the L. monocytogenes and B. cereus strains, respectively. Regular surveillance is needed to monitor the prevalence and AMR profiles of foodborne pathogens to prevent potential foodborne outbreaks, influence regulatory and policy changes, plan AMR stewardship programmes, and maintain the confidence of consumers in meats for sale.

食源性病原体构成重大食品安全风险,了解它们的存在和耐药性模式对公共卫生干预至关重要。本研究评估了来自加纳库马西大都会库马西屠宰场和Kejetia市场的生牛肉样本中选定食源性病原体的流行情况和抗生素耐药性。对牛肉样品进行蜡样芽孢杆菌、单核增生李斯特菌和大肠杆菌的检测。进行抗生素药敏试验以确定耐药谱。研究结果显示,病原菌的检出率各不相同,蜡样芽孢杆菌检出率为27.55%,单核增生乳杆菌检出率为55.41%,大肠杆菌检出率为59.91%。大约76%的样品至少含有三种细菌中的一种,微生物负荷高于细菌特异性可接受标准。大部分大肠杆菌ESBL阳性,蜡样芽孢杆菌和单核增生乳杆菌均为多药耐药,多重耐药指数均大于0.2。单核增生乳杆菌和蜡样芽孢杆菌菌株分别出现9种和4种AMR模式。需要定期监测食源性病原体的流行情况和抗菌素耐药性概况,以防止潜在的食源性疫情,影响监管和政策变化,规划抗菌素耐药性管理规划,并保持消费者对出售肉类的信心。
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引用次数: 0
Microencapsulation Techniques for Probiotic Formulations: Current Scenario and Future Perspective 微胶囊技术用于益生菌制剂:现状和未来展望
IF 2.9 3区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2025-08-30 DOI: 10.1155/jfq/6738124
Tawseefa Jan, Rajeshwari Negi, Arbina Hilal, Simranjeet Kaur, Divjot Kour, Sofia Sharief Khan, Sheikh Shreaz, Praneet Chauhan, Neelam Yadav, Sarvesh Rustagi, Ashutosh Kumar Rai, Sangram Singh, Ajar Nath Yadav, Naseer Ahmed

Probiotics, beneficial microorganisms that can enhance human health, are susceptible to adverse effects from processing and storage and passage through the gastrointestinal tract. Various conventional and nonconventional formulations are accessible for administering probiotics, with the latter subject to fewer regulatory guidelines. Pharmaceutical formulations, part of the conventional category, are specifically crafted to deliver an effective quantity of viable microorganisms. However, probiotics are particularly sensitive to environmental and processing factors, which can compromise their viability within the final formulation. This review addresses a significant gap in the current literature by focussing on the encapsulation technologies developed to enhance the stability of probiotics throughout the storage period, providing physical protection through the digestive system and improving their colonisation ability. Key advancements in encapsulation processes, including innovative approaches, thermal treatments and strategies to overcome gastrointestinal conditions, are discussed in this review.

益生菌是一种可以增强人体健康的有益微生物,在加工、储存和通过胃肠道的过程中容易受到不利影响。各种常规和非常规配方可用于管理益生菌,后者受到较少的监管准则。药物制剂,传统类别的一部分,是专门制作以提供有效数量的活微生物。然而,益生菌对环境和加工因素特别敏感,这可能会损害它们在最终配方中的生存能力。这篇综述解决了目前文献中的一个重大空白,重点介绍了开发的胶囊技术,以提高益生菌在整个储存期间的稳定性,通过消化系统提供物理保护并提高其定植能力。本文综述了封装工艺的主要进展,包括创新方法、热处理和克服胃肠道疾病的策略。
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引用次数: 0
A Comprehensive Multicriteria Assessment of Frozen and Cold Storage Systems on Quality of Benthic or Pelagic Fish 冷冻和冷藏系统对底栖或远洋鱼类品质的综合多准则评估
IF 2.9 3区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2025-08-27 DOI: 10.1155/jfq/5557956
Gonca Alak, Rabia Nur Yavaş, Ayşe Kara, Sevda Urçar Gelen, Arzu Ucar, Nurinisa Esenbuğa, Rasheda Chowdhury

Efforts to improve food safety on a global scale have become a topic of focus in recent years. Different preservation techniques, including new technological methods, are constantly being developed to keep products at physically, nutritionally, and microbially consumable levels and to prevent losses. In this study, the deep-freezing technique, which is widely used in the food industry, was applied to four marine fish species with different habitats and fat content (whiting [Merlangius merlangus], sea bass [Dicentrarchus labrax], red mullet [Mullus barbatus], and anchovy [Engraulis encrasicolus]). The study aimed to determine the effects of three-month storage period (−20°C) on some quality parameters (water activity [aw], water holding capacity [WHC], myofibril fragmentation index [MFI], color [L, a and b], total volatile base nitrogen [TVB-N], thiobarbituric acid reactive substance [TBARS], and pH) in fish and the interactions between the main effects. The experiment design included two-way ANOVA to evaluate the influence of species, storage time, and their interaction on each parameter. In line with the data obtained, significant differences were detected among the fish for all parameters except TVB-N. In terms of storage time, all parameters except a, TBARS, and fat were found to be statistically significant. All interactions between fish type and storage time, except fat and TVB-N, were found to be significant. Notably, benthic fish species exhibited higher WHC and lower lipid oxidation compared to pelagic species. Fatty fish such as anchovy and red mullet showed increased TBARS levels, indicating greater susceptibility to lipid oxidation. MFI values varied significantly among species and storage times, with sea bass showing the highest degree of myofibrillar fragmentation, suggesting enhanced tenderness. Correlation analysis revealed that WHC positively correlated with aw, L, and MFI and negatively with TVB-N. The research findings were planned to fill important gaps regarding the effect of freezing technology on fillet quality according to fish species. The findings obtained in the current research constituted a modeling study and created a useful data library for elucidating the mechanism by which fish species and habitat may affect fillet quality. These results contribute to the development of species-specific freezing strategies and provide practical insights for improving frozen seafood quality in the food industry. Moreover, these results provide a strong motivation to further investigate fish species and habitats with different processing technologies in terms of fillet quality in food industries.

近年来,在全球范围内努力提高食品安全已成为一个焦点话题。不同的保存技术,包括新的技术方法,正在不断发展,以保持产品在物理,营养和微生物可消耗的水平,并防止损失。本研究将食品工业中广泛应用的深度冷冻技术应用于四种不同生境和脂肪含量的海洋鱼类(白鲑[Merlangius merlangus]、鲈鱼[Dicentrarchus labrax]、红鲻鱼[Mullus barbatus]和凤尾鱼[Engraulis encrasicolus])。研究了3个月(- 20℃)贮藏期对鱼体内水分活度[aw]、持水量[WHC]、肌原纤维断裂指数[MFI]、颜色[L∗,a∗和b∗]、总挥发性碱氮[TVB-N]、硫代巴比托酸活性物质[TBARS]和pH值等品质参数的影响及其主要影响之间的相互作用。实验设计采用双因素方差分析来评估品种、储存时间及其相互作用对各参数的影响。根据获得的数据,除TVB-N外,所有参数在鱼之间都存在显著差异。在储存时间方面,除a *、TBARS和脂肪外,所有参数均具有统计学意义。除脂肪和TVB-N外,鱼类种类与储存时间的交互作用均显著。值得注意的是,与远洋鱼类相比,底栖鱼类表现出更高的WHC和更低的脂质氧化。富含脂肪的鱼类,如凤尾鱼和红鲻鱼,TBARS水平升高,表明对脂质氧化更敏感。MFI值因种类和储存时间的不同而有显著差异,黑鲈肌纤维断裂程度最高,表明柔嫩程度增强。相关分析显示,WHC与aw、L *、MFI呈正相关,与TVB-N负相关。研究结果旨在填补冷冻技术对鱼片质量影响方面的重要空白。本研究的结果构成了一个模型研究,为阐明鱼类种类和生境对鱼片质量的影响机制提供了一个有用的数据库。这些结果有助于开发特定物种的冷冻策略,并为提高食品工业中冷冻海鲜的质量提供实用见解。此外,这些结果为进一步研究不同加工工艺的鱼类种类和栖息地对食品工业中鱼片质量的影响提供了强大的动力。
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引用次数: 0
Effects of Fuzhuan Brick Tea Supplemented With Areca Nuts on Serum and Hepatic Lipid Metabolism in Mice 附转砖茶加槟榔对小鼠血清和肝脏脂质代谢的影响
IF 2.9 3区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2025-08-26 DOI: 10.1155/jfq/1191562
Ming-Xi Zhou, Xing Tian, Zhong-Qin Wu, Ke Li, Zong-Jun Li

Objectives: Both areca nut and Fuzhuan brick tea have shown the potential to help people lose weight. This paper aims to study the effects of adding 20% (w/w) concentration of areca nut (FTA) in Fu brick tea on the serum and liver lipid metabolism of mice to offer new ideas for the treatment of obesity and hyperlipidemia and to provide a theoretical basis for the safe use and future development of the areca nut products.

Results: FTA may have reduced weight; prevented fat accumulation; attenuated the increase in LDL-C, FFA, GLU, INS, LEP, TC, TG, and MDA; and alleviated the reduction in HDL-C, TBA, SOD, and GSH. Furthermore, according to GO functional enrichment analysis, FTA may have also reversed the negative effects of a high-fat diet by upregulating the expression of FABP2, ACSL1, ACSL5, PLIN2, PLIN5, and ACOX1.

Conclusion: FTA may favorably reverse the effects of a high-fat diet on physiological indices, blood indices, and hepatic lipid metabolism. Furthermore, at the genetic level, FTA may suppress body fat accumulation, improve lipid metabolism, relieve insulin resistance, and reduce chronic metabolic disease incidence via the PPAR signaling pathway.

目的:槟榔果和扶正砖茶都显示出帮助人们减肥的潜力。本文旨在研究在茯砖茶中添加20% (w/w)浓度的槟榔(FTA)对小鼠血清和肝脏脂质代谢的影响,为治疗肥胖和高脂血症提供新思路,为槟榔制品的安全使用和未来开发提供理论依据。结果:FTA可减轻体重;防止脂肪堆积;降低LDL-C、FFA、GLU、INS、LEP、TC、TG、MDA的升高;减轻了HDL-C、TBA、SOD和GSH的降低。此外,根据氧化石墨烯功能富集分析,FTA还可能通过上调FABP2、ACSL1、ACSL5、PLIN2、PLIN5和ACOX1的表达来逆转高脂肪饮食的负面影响。结论:FTA可逆转高脂饮食对生理指标、血液指标和肝脏脂质代谢的影响。此外,在遗传水平上,FTA可能通过PPAR信号通路抑制体脂积累,改善脂质代谢,缓解胰岛素抵抗,降低慢性代谢性疾病的发病率。
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引用次数: 0
Enhancing Cookie Quality Through the Incorporation of Germinated Chia Seed (Salvia hispanica L.) Flour: A Sustainable Bioprocessing Strategy 添加发芽奇亚籽提高饼干品质的研究面粉:可持续生物加工策略
IF 2.9 3区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2025-08-19 DOI: 10.1155/jfq/2890951
Tanjim Ahmed, Md. Rakibul Islam, Md. Hasan Tarek Mondal, Mrityunjoy Biswas, Qazi Shabab Mahmood, Pallab Biswas, Md. Akhtaruzzaman

The rising demand for nutrient-dense, functional food products has urged the expansion of sustainable bioprocessing techniques. One such method, germination, involves the controlled application of biological processes to transform seeds into germinated seeds. This process improves composite flours’ nutritional and technological properties, making them a promising component for innovative food products. Chia seeds, a popular superfood, are a possible substitute raw material in the food industry due to their high nutritive value. Additionally, they can produce a substantial amount of nutrient-rich green matter, which can be utilized as a source of biologically active substances. Considering the rising popularity of germination and the known health benefits of chia seeds, this study explored the impact of germinated chia seed flour (GCSF) and nongerminated chia seed flour (NCSF) on wheat flour-based cookies. The GCSF was prepared by sprouting chia seeds for 5 days, followed by drying and grinding into a fine powder. The study evaluated the impact of GCSF on the cookies’ nutritional profile, physicochemical properties, technological features, and organoleptic attributes. Cookies were prepared by formulating wheat flour with 10% GCSF (T1), 10% NCSF (T2), and 5% GCSF + 5% NCSF (T3), while the control sample (T0) was prepared using 100% wheat flour. The addition of GCSF into cookies notably (p < 0.05) enhanced their WAC (0.94 g H2O/g dry sample), OAC (0.97 g oil/g dry sample), crude protein (13.48%), and crude fiber (5.24%) content. Conversely, cookies formulated with NCSF exhibited elevated levels (p < 0.05) of crude fat (23.92%) and total carbohydrates (54.07%). However, the overall consumer acceptance of chia seed–based cookies was slightly diminished when using GCSF, as evidenced by the principal component analysis (PCA).

对营养丰富的功能性食品日益增长的需求促使了可持续生物加工技术的发展。其中一种方法,发芽,包括控制应用生物过程将种子转化为发芽的种子。这一过程改善了复合面粉的营养和技术特性,使其成为创新食品的有前途的成分。奇亚籽是一种受欢迎的超级食品,由于其高营养价值,在食品工业中是一种可能的替代原料。此外,它们可以产生大量富含营养的绿色物质,这些绿色物质可以用作生物活性物质的来源。考虑到奇亚籽的发芽和已知的健康益处,本研究探讨了发芽奇亚籽粉(GCSF)和未发芽奇亚籽粉(NCSF)对小麦粉饼干的影响。将奇亚籽发芽5 d,干燥磨成细粉制备GCSF。该研究评估了GCSF对饼干的营养成分、理化性质、工艺特征和感官属性的影响。采用10% GCSF (T1)、10% NCSF (T2)和5% GCSF + 5% NCSF (T3)的小麦粉配制饼干,对照样品(T0)采用100%小麦粉配制。GCSF显著提高了饼干的WAC (0.94 g H2O/g干样)、OAC (0.97 g oil/g干样)、粗蛋白质(13.48%)和粗纤维(5.24%)含量(p < 0.05)。相反,添加NCSF的饼干的粗脂肪(23.92%)和总碳水化合物(54.07%)含量升高(p < 0.05)。然而,当使用GCSF时,总体消费者对奇亚籽饼干的接受程度略有降低,主成分分析(PCA)证明了这一点。
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引用次数: 0
Determination of Four Cholinomimetics in Edible Fungi and Areca Nut Using Ultra-High Performance Liquid Chromatography Combined With Quadrupole/Time-of-Flight Mass Spectrometry 超高效液相色谱-四极杆/飞行时间质谱联用测定食用菌和槟榔中4种胆碱类物质
IF 2.9 3区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2025-08-18 DOI: 10.1155/jfq/7038821
Junmei Ma, Qiang Li, Yonghuan Yan, Lei Sun, Pin Lv, Yan Zhang

Background: Cholinomimetic compounds are a class of organic compounds found in natural plants that have significant biological activity as well as toxicity, and their dosage must be controlled, with side effects occurring in case of accidental ingestion or excessive intake. Accurate determination of four cholinomimetic compounds in edible fungi and areca nut can provide technical support to the relevant monitoring organizations, which is conducive to the protection of consumer health. In this study, we have established a workflow for the rapid screening and confirmation of four cholinomimetics in edible fungi and areca nut by using the QuEChERS pretreatment method and ultra-high performance liquid chromatography coupled with quadrupole/time-of-flight mass spectrometry (UPLC-Q-TOF/MS) for the first time.

Methods: The samples were extracted with acetonitrile, and separation was achieved on a HILIC column (2.1 mm × 100 mm, 1.6 μm) with acetonitrile and 20 mM ammonium formate aqueous solution as mobile phase with gradient elution. UPLC-Triple TOF™6600+ was used to collect the data of the target analytes in the mode of TOF-MS-IDA-MS/MS, and the data were analyzed by SCIEX OS software to realize the qualitative screening and confirmation of samples.

Results: The mass accuracy error of 4 cholinomimetics was lower than 5.0 × 10−6. All four cholinomimetic compounds had good linearity in a certain range, and the correlation coefficient (R2) was higher than 0.998. The limits of detection and limits of quantification of the method were 2.5–25.0 μg/kg and 5.0–50.0 μg/kg, respectively. The recoveries were between 81.0% and 99.1% at spiked level 5.0–500.0 μg/kg, with the relative standard deviation of 0.41%–4.00%.

Conclusion: The method is sensitive, accurate, and suitable for rapid screening and confirmation of 4 cholinomimetics in edible fungi and areca nut.

背景:拟胆碱化合物是一类天然植物中发现的具有显著生物活性和毒性的有机化合物,其剂量必须控制,误食或过量摄入会产生副作用。准确测定食用菌和槟榔中4种类胆碱化合物,可为相关监测机构提供技术支持,有利于保护消费者健康。本研究首次采用QuEChERS预处理方法和超高效液相色谱-四极杆/飞行时间质谱联用(UPLC-Q-TOF/MS)技术,建立了食用菌和槟榔中4种胆碱模拟物的快速筛选和鉴定流程。方法:用乙腈提取样品,以乙腈和20 mm甲酸铵水溶液为流动相,采用梯度洗脱,采用HILIC柱(2.1 mm × 100 mm, 1.6 μm)分离。采用UPLC-Triple TOF™6600+,以TOF-MS- ida -MS/MS模式采集目标分析物数据,采用SCIEX OS软件对数据进行分析,实现样品的定性筛选和确认。结果:4种仿胆碱制剂的质量精度误差均小于5.0 × 10−6。4种拟胆碱类化合物在一定范围内线性良好,相关系数(R2)均大于0.998。方法的检出限为2.5 ~ 25.0 μg/kg,定量限为5.0 ~ 50.0 μg/kg。加标水平为5.0 ~ 500.0 μg/kg时,加标回收率为81.0% ~ 99.1%,相对标准偏差为0.41% ~ 4.00%。结论:该方法灵敏、准确,适用于食用菌和槟榔中4种拟胆碱物质的快速筛选和确证。
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引用次数: 0
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Journal of Food Quality
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