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Development of loop mediated isothermal amplification for rapid species detection of Armillaria ostoyae using assimilating probe 利用同化探针建立环介导等温扩增快速检测蜜环菌种
IF 1.1 Q3 FORESTRY Pub Date : 2022-05-19 DOI: 10.17221/161/2021-jfs
T. Tonka, Dagmar Stehlíková, L. Walterová, V. Čurn
We introduced here the first loop mediated isothermal amplification (LAMP) assay for the identification of honey fungus, Armillaria ostoyae, a basidiomycote playing an important role in spruce declines in the Palaearctic region. In total, 101 isolates, representing three Armillaria species, were used to develop a new LAMP assay to determine species specific identification. We have here described LAMP primers enhanced with fluorescent dye that are able to amplify A. ostoyae DNA and detect fungi in a fast single step reaction. The detection limit of LAMP was 1 pg of genomic DNA per reaction. We optimized a new LAMP assay for the rapid detection of A. ostoyae using the translation elongation factor 1-α (tef1) marker and fluorescence labelled oligonucleotide assimilating probe. The LAMP assay does not require any specialized equipment, hence it can be used in the field for the rapid detection of A. ostoyae even using the portable and mobile device. The specificity of the assay was confirmed by the use of A. ostoyae strains and Armillaria cepistipes and Armillaria gallica strains, respectively. In conclusion, the assay could be a rapid, specific, sensitive and low-cost tool for identification of A. ostoyae as well as the first step for expansion of this method in practical applications.
本文首次采用环介导等温扩增(LAMP)方法鉴定了古北地区云杉退化中起重要作用的蜜环菌蜜环菌。总共101株蜜环菌,代表3个蜜环菌种,建立了一种新的LAMP方法来确定种特异性鉴定。我们在这里描述了用荧光染料增强的LAMP引物,能够扩增a . ostoyae DNA并在快速的单步反应中检测真菌。LAMP的检出限为每次反应1pg基因组DNA。利用翻译延伸因子1-α (tef1)标记和荧光标记的寡核苷酸同化探针,优化了一种新的快速检测羊角霉的LAMP方法。LAMP法不需要任何专门的设备,因此即使使用便携式和移动设备,也可以在现场快速检测古伊螨。结果表明,该方法具有较好的特异性,并分别以牛耳蜜环菌、牛耳蜜环菌和高卢蜜环菌为检测对象。该方法可作为一种快速、特异、灵敏、低成本的鉴定方法,为进一步扩大该方法的实际应用奠定基础。
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引用次数: 0
Behaviours and attitudes of consumers towards bioplastics: An exploratory study in Italy 消费者对生物塑料的行为和态度:意大利的一项探索性研究
IF 1.1 Q3 FORESTRY Pub Date : 2022-04-21 DOI: 10.17221/26/2022-jfs
S. Notaro, A. Paletto, Elisabetta Lovera
Bio-based and biodegradable plastics produced from wood residues can have a positive impact on the environment by replacing conventional plastics. However, the current bioplastics market is held back by a lack of available information and weak marketing activities aimed at final consumers. To increase the available information, the present study investigated the consumers’ attitudes and behaviours towards bioplastic products. A web-based survey was conducted on a sample of potential consumers in Italy. 1 115 consumers filled out the questionnaire with a dropout rate in compilation of 14%. The results showed that the environmental characteristics of bioplastics (lower impact on climate change and renewable sources used to produce them) are considered more important by respondents than the non-environmental characteristics (technical properties, origin of raw material, potential trade-off between bioplastics and food production). The results highlighted that the most important behavioural factor is the purchase intentions, followed by control of perceived cost and subjective norm. It is interesting to emphasize that the cost of bioplastics compared to conventional plastics is a key variable in the choices of many Italian consumers. The results provided can be useful to the manufacturing industries to better understand the consumers’ attitudes towards bioplastics.
由木材残留物生产的生物基和可生物降解塑料可以取代传统塑料,对环境产生积极影响。然而,由于缺乏可用信息和针对最终消费者的营销活动薄弱,目前的生物塑料市场受到阻碍。为了增加可获得的信息,本研究调查了消费者对生物塑料产品的态度和行为。对意大利的潜在消费者样本进行了网络调查,1115名消费者填写了问卷,编制的辍学率为14%。结果表明,受访者认为生物塑料的环境特性(对气候变化的影响较小以及用于生产生物塑料的可再生资源)比非环境特性(技术特性、原材料来源、生物塑料与食品生产之间的潜在权衡)更重要。结果显示,最重要的行为因素是购买意愿,其次是感知成本控制和主观规范。值得强调的是,与传统塑料相比,生物塑料的成本是许多意大利消费者选择的一个关键变量。提供的结果可以帮助制造业更好地了解消费者对生物塑料的态度。
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引用次数: 1
Evaluation of selected growth parameters of Paulownia cotevisa plantation in the Danubian Lowland 多瑙河低地泡桐人工林生长参数选择评价
IF 1.1 Q3 FORESTRY Pub Date : 2022-03-24 DOI: 10.17221/155/2021-jfs
M. Pástor, Jaroslav Jankovič, Martin Belko, J. Modranský
The main objective of this study was to assess the growth of the established Paulownia cotevisa plantation during an extended time period and compare it with values reviewed in the literature. Seven years after planting, mean diameter at breast height and height of the aboveground part of P. cotevisa 2® (P. cotevisa) plantation were similar to values reported in the literature and they reached 21.5 cm and 11.2 m, respectively. Besides the crown damage caused by wind, development of the P. cotevisa plantation established in the Danubian Lowland was not affected by any other harmful environmental factor or biological pest. The results suggest that P. cotevisa could be used to a larger extent in diversification of biomass production on abandoned arable lands of the Danubian Lowland.
本研究的主要目的是评估泡桐人工林在较长时间内的生长情况,并将其与文献中评述的值进行比较。种植7年后,白杨2®(P. cotevisa®)人工林胸脯高度和地上部分高度的平均直径与文献报道值相近,分别达到21.5 cm和11.2 m。在多瑙河低地建立的白杨人工林,除风对林冠造成的破坏外,不受其他有害环境因子和生物害虫的影响。结果表明,在多瑙河低地撂荒耕地上,cotevisa可在更大程度上实现生物质生产的多样化。
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引用次数: 0
Burned area determination using Sentinel-2 satellite images and the impact of fire on the availability of soil nutrients in Syria 利用Sentinel-2卫星图像确定叙利亚的烧伤面积以及火灾对土壤养分可用性的影响
IF 1.1 Q3 FORESTRY Pub Date : 2022-03-22 DOI: 10.17221/122/2021-jfs
Rukea Al-hasn, Raed Almuhammad
The objective of this research is the identification of burned forest areas that occurred in Syria from September 2nd to October 15th, 2020. Forest fire risk classes were determined using Sentinel-2 images. Normalized Burn Ratio (NBR), Differenced Normalized Burn Ratio (dNBR), and Burned Area Index for Sentinel-2 (BAIS2), and Normalized Difference Vegetation Index (NDVI) were used for the identification how much the forests have been destroyed and to establish fire risk classes. According to the study results, the size of the vegetation area that was destroyed due to fire was determined, and the probability of the forest fire exposure of these areas was established. The fires also altered some chemical properties in the soil during the combustion process. Thus, this study was focused on the impact of fire on the availability of soil nutrients. Soil samples were collected from three depths (0–10 cm, 10–20 cm and 20–30 cm) under the forest land a month after the fire in three locations: Al-Fazeen, Sawda and Gard Al-rihan. Pine (Pinus brutia) trees cover these areas. The results of this study indicated that the fire increased pH, EC and sand, the fire also led to an increase in the solubility of the available major soil elements N, P and K. There was an increase in the solubility of the soil microelements Zn, Cu, Mn and Fe while the content of organic material and silt and clay ratio decreased at the three sites in comparison with unburned soil.
本研究的目的是确定2020年9月2日至10月15日在叙利亚发生的烧毁森林地区。使用Sentinel-2图像确定森林火灾风险等级。采用归一化燃烧比(NBR)、差异归一化燃烧比(dNBR)、Sentinel-2的烧毁面积指数(BAIS2)和归一化植被指数(NDVI)来识别森林的破坏程度并建立火灾风险等级。根据研究结果,确定了因火灾而被破坏的植被面积大小,并建立了这些区域的森林火灾暴露概率。在燃烧过程中,大火还改变了土壤中的一些化学性质。因此,本研究的重点是火灾对土壤养分有效性的影响。在火灾发生一个月后,在Al-Fazeen、Sawda和Gard Al-rihan三个地点的林地下三个深度(0-10厘米、10-20厘米和20-30厘米)采集了土壤样本。松树(Pinus brutia)覆盖了这些地区。结果表明:与未燃烧的土壤相比,3个地点土壤中微量元素Zn、Cu、Mn、Fe的溶解度有所增加,有机质含量和粉土比均有所下降,土壤有机质含量和粉土比均有所下降。
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引用次数: 2
Changes of tree stem biomass in European forests since 1950 1950年以来欧洲森林树干生物量的变化
IF 1.1 Q3 FORESTRY Pub Date : 2022-03-15 DOI: 10.17221/135/2021-jfs
A. Lebedev, V. Kuzmichev
Based on the measurements of the biomass of the stems of 3 699 trees of Scots pine, Norway spruce, and silver birch in Europe since 1950, it has been shown that these tree species show a reduction in biomass and wood density. These results contradict the fact that the volume of wood is directly converted to biomass using the historical values of the conversion rates. From 1950 to 2020 the biomass of 1 m3 of the stem with bark decreased on average by 80 kg (–17%) for Scots pine, by 105 kg (–22%) for Norway spruce and by 92 kg (–15%) for silver birch. The results obtained should be taken into account when assessing the technical properties of wood and estimating carbon sequestration by forest biomass. Since decreasing trends in stem biomass have been identified for several tree species, the phenomenon may have a large degree of generality. Such studies should be continued both at the regional and national level and at the global level.
根据1950年以来对欧洲3699棵苏格兰松、挪威云杉和白桦树的茎生物量的测量,表明这些树种的生物量和木材密度都在减少。这些结果与使用转化率的历史值直接将木材体积转化为生物质的事实相矛盾。从1950年到2020年,1立方米带树皮的茎的生物量平均减少了80公斤(-17%),挪威云杉减少了105公斤(-22%),白桦减少了92公斤(-15%)。在评估木材的技术特性和估算森林生物量的固碳量时,应考虑到所获得的结果。由于一些树种的茎生物量呈下降趋势,因此这种现象可能具有很大程度的普遍性。这种研究应在区域和国家一级以及在全球一级继续进行。
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引用次数: 2
Contribution of the forest sector to the Uruguayan economy: A first approach with National Accounts 森林部门对乌拉圭经济的贡献:国民核算的第一种方法
IF 1.1 Q3 FORESTRY Pub Date : 2022-03-11 DOI: 10.17221/149/2021-jfs
V. Morales, Ernesto Pienika
The forest sector development in Uruguay has been based on planted forest areas, mainly with eucalyptus and pine. The Parliament passed the Forestry Law in 1987 and since then the sector has been growing rapidly. The objective of the study is to analyse the contribution of the forest sector in the Uruguayan economy, its evolution between 1997 and 2018 and its distribution in recent years. The research used information from the new National Accounts released by the Central Bank of Uruguay, and, in this framework, the forest sector is studied for the main macroeconomic variables and production. The results show that in the period 1997–2018, while the Uruguayan economy grew 2.6% in constant terms, on average, the forest sector grew 7.6% on average, with peaks in 2007–2008 and 2013–2015. In 2016, the forest production was allocated to exports (46.76%), which surpassed intermediate use (43.05%) after initial operations of the second pulp mill in the country. Furthermore, forest exports represented 10.5% of the country’s total exports of goods and services, showing an exporter profile.
乌拉圭森林部门的发展以种植林区为基础,主要种植桉树和松树。议会于1987年通过了《森林法》,自那时以来,该部门一直在迅速发展。该研究的目的是分析森林部门在乌拉圭经济中的贡献,其1997年至2018年的演变及其近年来的分布。这项研究使用了乌拉圭中央银行公布的新的国民核算资料,在这个框架内,研究了森林部门的主要宏观经济变量和产量。结果表明,在1997年至2018年期间,乌拉圭经济平均增长2.6%,而森林部门平均增长7.6%,2007年至2008年和2013年至2015年达到峰值。2016年,森林生产分配给出口(46.76%),超过了该国第二家纸浆厂初始运营后的中间用途(43.05%)。此外,森林出口占该国货物和服务出口总额的10.5%,显示出出口国的概况。
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引用次数: 0
Growth response of seven multipurpose tree species to climatic factors: A case study from northwestern Himalayas, India 7种多用途树种生长对气候因子的响应——以印度喜马拉雅山西北部为例
IF 1.1 Q3 FORESTRY Pub Date : 2022-03-08 DOI: 10.17221/159/2021-jfs
S. Panda, D. Bhardwaj, C. Thakur, Prashant Sharma, Dhirender Kumar
Identification of the species for dendrochronological studies is of great relevance to understand various aspects of climate change. However, in the northwestern Himalayan region, dendroclimatological investigations are confined to conifer species, with broadleaved species being disregarded. Thus, the present study was conducted to assess the growth response of seven multipurpose tree species (MPTs), namely Bauhinia variegata, Celtis australis, Grewia optiva, Paulownia fortunei, Toona ciliata, Ulmus villosa and Melia composita to local climate variables, viz. temperature as well as rainfall (seasonal, monthly, average) and CO2 level by evaluating the climatic signal in tree ring chronologies at Solan district, India (altitude 1 250 m) in the mid-hills of the northwestern Himalayas. The results indicated that only the maximum, rainy season temperature and CO2 level varied significantly (P < 0.05) between 1991 and 2017. Only G. optiva exhibited a significant (P < 0.05) tendency toward increased growth. C. australis has a remarkable negative correlation with temperature variables, viz. average, maximum, spring season, March temperature, whereas T. ciliata exhibits a positive correlation with temperature variables, such as rainy season, average and April temperature. Similarly, winter, total and December rainfall have a profound effect on P. fortunei, while March rainfall adversely affected the growth of B. variegata. On the other hand, G. optiva demonstrated sensitivity to both temperature (February and May) and rainfall variables (winter, February and May). U. villosa recorded a positive correlation with rainfall (autumn and October rainfall) but a negative correlation with temperature variables (maximum and April temperature). Elevated CO2 levels affected only two species (G. optiva, M. composita) out of the seven selected species. Our findings will contribute to a better understanding of the climate growth relationships of investigated tree species, as a result, to more accurate projections of the effects of climate change on these MPTs and directing future studies.
确定树木年代学研究的物种对了解气候变化的各个方面具有重要意义。然而,在西北喜马拉雅地区,树木气候学调查仅限于针叶树种,而阔叶树种被忽视。为此,本研究以印度索兰地区(海拔1250 m)为研究对象,通过树木年轮年表的气候信号,评估了7种多用途树种(紫荆花、南芹、青叶、泡桐、香椿、榆木和复合木犀)的生长对温度、降水(季节、月、平均)和CO2水平的响应。结果表明:1991 ~ 2017年,只有最高气温、雨季气温和CO2水平变化显著(P < 0.05);只有青花有显著(P < 0.05)的增长趋势。南菖蒲与平均、最高、春季、3月气温呈显著负相关,而纤毛菖蒲与雨季、平均、4月气温呈显著正相关。同样,冬季、全年和12月的降雨量对毛毡的生长有深远的影响,而3月的降雨量对毛毡的生长有不利影响。另一方面,对温度(2月和5月)和降雨(冬季、2月和5月)均表现出敏感性。与降雨量(秋季和10月降雨量)呈正相关,与气温(最高气温和4月气温)呈负相关。CO2浓度升高只影响了7个选择物种中的2个物种(G. optiva, M. composita)。我们的发现将有助于更好地理解所调查树种的气候生长关系,从而更准确地预测气候变化对这些mpt的影响,并指导未来的研究。
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引用次数: 2
What does the mandible say about wild boar: Ontogenetic development, sexual dimorphism and habitat preferences 下颌骨说明了野猪的什么:个体发育、两性二态性和栖息地偏好
IF 1.1 Q3 FORESTRY Pub Date : 2022-02-21 DOI: 10.17221/128/2021-jfs
Klára Košinová, R. Policht, O. Matějka, V. Hanzal, M. Ježek, T. Kušta, V. Hart
Wild boar (Sus scrofa L.) is one of the most discussed game species, distributed across Europe, therefore the management of this species is considered important. This management should be based on data presented, population quality and preferences and craniometric dimensions show the development of the individual and its prosperity. A sample of 148 male and 153 female wild boar mandibles was studied to compare differences in craniometric measurements, especially to find out wild boar environmental demands and population trends. The width of the caput mandibulae and angle of the mandible showed significant difference between males and females. Measurements analysed with forest area size and other data also showed that larger craniometric dimensions were reached in hunting areas with at least 200 ha of forested area, which may be due to the wild boar’s need for safety and vegetative cover in the first months of piglet development with respect to its home range. The development of young wild boar is dependent on rest and shelter in the first months of life. A forest cover of at least 200 ha appears to be sufficient in this respect. Information on habitat preferences and individual development can lead to improvements in wild boar management.
野猪(Sus scrofa L.)是讨论最多的狩猎物种之一,分布在欧洲各地,因此对该物种的管理被认为是重要的。这种管理应基于所提供的数据、人口质量和偏好以及显示个人发展及其繁荣程度的颅骨测量尺寸。研究了148个雄性和153个雌性野猪的下颌骨样本,比较了颅骨测量的差异,特别是了解了野猪的环境需求和种群趋势。下颌骨的宽度和下颌骨的角度在男性和女性之间有显著差异。根据森林面积大小和其他数据分析的测量结果还表明,在森林面积至少为200公顷的狩猎区,达到了更大的颅骨尺寸,这可能是由于野猪在仔猪发育的头几个月需要安全和植被覆盖,相对于其家园范围。野猪幼崽在出生后的头几个月依赖于休息和庇护。在这方面,至少200公顷的森林覆盖似乎就足够了。关于栖息地偏好和个体发展的信息可以改善野猪的管理。
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引用次数: 0
Time study of an all-terrain vehicle (ATV) with a forwarding trailer in the Eagle Mountains in the Czech Republic 全地形车(ATV)的时间研究与前进拖车在鹰山在捷克共和国
IF 1.1 Q3 FORESTRY Pub Date : 2022-02-21 DOI: 10.17221/148/2021-jfs
Zbyněk Kincl
In this research, the time consumption of a CF Moto Gladiator 800 all-terrain vehicle (ATV) with a Vahva Jussi 1500/320 log trailer was researched by the timber forwarding in the forests of the Eagle Mountains in the Czech Republic. The study was conducted in an area with a minimal slope, the terrain was full of obstacles, the average forwarding distance was 480 m, while the random harvesting of trees affected by bark beetle was carried out in the forest. A total of 81 forwarding cycles were measured. The elemental time study method and stepwise regression analysis were applied to develop the forwarding time prediction model. In the study, it was found that the total forwarding cycle time is mostly influenced by the forwarding distance and the number of logs loaded. The total average time per forwarding cycle was 22.53 min, of which an average of 40% was transport time and 60% was time for loading and unloading of logs. An average of 1.43 m3 of timber was forwarded per forwarding cycle. Therefore, the estimated productivity of the ATV forwarder was 3.89 m3·h–1.
本研究以一辆CF Moto Gladiator 800全地形车(ATV)和一辆Vahva Jussi 1500/320原木拖车为研究对象,在捷克共和国Eagle Mountains的森林中进行木材运输。本研究选取坡度最小的区域,地形充满障碍物,平均前进距离为480 m,在森林中随机采伐受树皮甲虫影响的树木。共检测到81个转发周期。采用元素时间研究方法和逐步回归分析建立了转发时间预测模型。研究发现,总的转发周期时间主要受转发距离和日志加载数量的影响。每个转发周期的总平均时间为22.53 min,其中平均40%为运输时间,60%为日志装卸时间。每个转发周期平均转发1.43立方米的木材。因此,ATV货代的生产率估计为3.89 m3·h-1。
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引用次数: 0
New technological solutions for waterlogged forests by cable yarding 水涝森林的电缆围场新技术解决方案
IF 1.1 Q3 FORESTRY Pub Date : 2022-02-21 DOI: 10.17221/94/2021-jfs
Artsiom Shoshyn, P. Protas, V. Štollmann
The article proposes new technological techniques for clear cutting in swampy areas using standing skylines, new construction of yarder and the technology of its operation. As a result of the research on using the Larix 3T-500 cable yarder in winter and summer, the operational negative peculiarities of tower yarder in waterlogged forests were identified. Analysing data on experimental comparative time studies, some measures to reduce the negative effect of the identified features on yarding, felling, lateral yarding were worked out. General recommendations for work performance in swampy logging areas were developed. Based on the initial data for each bundle (yarding distance, lateral yarding distance, average volume of the bundle, etc.) the calculation in the MathCad program was performed and a regression dependence of the yarder performance was obtained. Obtained numerical data on comparative time studies of the working cycle operations were summarized in bar charts, taking into account the loss of time on operations.
本文提出了利用立交线进行沼泽地区清伐的新技术、新工场的建设及其操作技术。通过对落叶松3T-500型索桁机冬季和夏季使用情况的研究,确定了塔桁机在涝林区的运行负特性。通过对试验对比时间研究数据的分析,提出了减少已识别特征对围垦、采伐、侧围垦的负面影响的措施。对沼泽伐木地区的工作绩效提出了一般性建议。根据每个管束的初始数据(码垛距离、横向码垛距离、管束平均体积等)在MathCad程序中进行计算,得到码垛性能的回归依赖关系。考虑到作业上的时间损失,以条形图的形式总结了在工作周期作业的比较时间研究中获得的数值数据。
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引用次数: 2
期刊
Journal of forest science
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