{"title":"Evaluation of Antibacterial Properties of Magnetic Iron Oxide Nanoparticles Synthesized using Echinops Persicus Extract Coated with Chloramphenicol","authors":"Reihaneh Ahmadieh, S. Mohseni","doi":"10.52547/sjimu.29.3.59","DOIUrl":"https://doi.org/10.52547/sjimu.29.3.59","url":null,"abstract":"","PeriodicalId":16041,"journal":{"name":"journal of ilam university of medical sciences","volume":"47 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87724412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mohamad Feizi, A. Behbahaninia, Saeed Reza Asemi Zavareh, N. Rostami
{"title":"Identification of a Suitable Place for Sanitary Landfill in Ilam","authors":"Mohamad Feizi, A. Behbahaninia, Saeed Reza Asemi Zavareh, N. Rostami","doi":"10.52547/sjimu.29.3.38","DOIUrl":"https://doi.org/10.52547/sjimu.29.3.38","url":null,"abstract":"","PeriodicalId":16041,"journal":{"name":"journal of ilam university of medical sciences","volume":"11 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72938010","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effectiveness of Mindfulness Training on EMotional Dysfunction and Academic Achievement of Junior Middle School Students","authors":"Meysam Mohebi, Majid Zargham hajebi, Nader Monirpoor","doi":"10.52547/sjimu.29.3.100","DOIUrl":"https://doi.org/10.52547/sjimu.29.3.100","url":null,"abstract":"","PeriodicalId":16041,"journal":{"name":"journal of ilam university of medical sciences","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86202650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Investigation of the Effects of Vitamin C on Resistance to 5-FU in Colon Cancer Cells Line HT29","authors":"A. Zarei, S. Ghorbian","doi":"10.52547/sjimu.29.3.49","DOIUrl":"https://doi.org/10.52547/sjimu.29.3.49","url":null,"abstract":"","PeriodicalId":16041,"journal":{"name":"journal of ilam university of medical sciences","volume":"42 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88834743","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"A Survey on the Observance rate of Research Ethics Principles in Approved Proposals at Ilam University of Medical Sciences, Ilam, Iran","authors":"A. Aivazi, M. Hoseiny-Rad, Mojtaba Parsa","doi":"10.52547/sjimu.29.3.12","DOIUrl":"https://doi.org/10.52547/sjimu.29.3.12","url":null,"abstract":"","PeriodicalId":16041,"journal":{"name":"journal of ilam university of medical sciences","volume":"56 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86939635","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Comparison of Antioxidant, Antibacterial, and Cytotoxic Effects of Essential Oil and Nanoemulsion of Clove Essential Oil","authors":"Atena Vafayi Malek, E. Karimi, E. Oskoueian","doi":"10.52547/sjimu.29.3.26","DOIUrl":"https://doi.org/10.52547/sjimu.29.3.26","url":null,"abstract":"","PeriodicalId":16041,"journal":{"name":"journal of ilam university of medical sciences","volume":"33 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88781451","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effects of oleuropein on malondialdehyde level and expression of \u0000GluN2B gene following passive avoidance memory impairment in mice","authors":"G. Mahmoodi, A. Moshfegh","doi":"10.52547/sjimu.29.3.92","DOIUrl":"https://doi.org/10.52547/sjimu.29.3.92","url":null,"abstract":"","PeriodicalId":16041,"journal":{"name":"journal of ilam university of medical sciences","volume":"31 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73935237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Phytochemical Investigation, Antioxidant Properties, and Identification\u0000 of Organic and Inorganic Compounds from the Carob (Ceratonia siliqua L.) Fruit","authors":"M. Nasiri, Babak Mokhtari, M. Kolahi, N. Pourreza","doi":"10.52547/sjimu.29.3.82","DOIUrl":"https://doi.org/10.52547/sjimu.29.3.82","url":null,"abstract":"","PeriodicalId":16041,"journal":{"name":"journal of ilam university of medical sciences","volume":"187 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74236400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mohsen Sharifirad, A. Poursaeed, F. Lashgarara, S. Mirdamadi
{"title":"A Survey on General Health Status of Paddy Field Workers in Mazandaran Province using the GHQ-28 Questionnaire","authors":"Mohsen Sharifirad, A. Poursaeed, F. Lashgarara, S. Mirdamadi","doi":"10.52547/sjimu.29.3.72","DOIUrl":"https://doi.org/10.52547/sjimu.29.3.72","url":null,"abstract":"","PeriodicalId":16041,"journal":{"name":"journal of ilam university of medical sciences","volume":"33 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90180700","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Atefeh Sadeghi Shermeh, S. Habibzadeh, Adeleh Taghikhani, S. Rafati, N. Seyed
Introduction: Dendritic cells are very important in basic studies and vaccine research, but isolation and culture of these cells face challenges due to their small number in tissues. Since there is no standard method, we addressed some of the factors affecting the efficiency of dendritic cell isolation and culture from BALB/c mouse bone marrow. Materials & Methods: Bone marrow cells isolated from femur and tibia, were seeded in plates and treated with GM-CSF. On day 3, half of the media was transferred to a new plate with fresh media plus GM-CSF. On day 5, cells were induced with CpG oligonucleotide. Cell survival as well as maturation markers of CD11C, MHC-II and CD86 were investigated by flow cytometry on day 5 (immature cells) and day 7 (mature cells). Ethics code: IR.PII.REC.1395.99 Findings: It was found that the presence of endotoxin in the culture of immature cells, significantly reduces the recovery of dendritic cells (p value <0.05). Less than one million cells seeded in non-treated 6-well plates and transfer of cells to a new plate on day 3 increased the efficiency of immature cell retrieval by preventing early maturation and premature death. Further treatment of immature cells with CpG on day 7 was used to induce the Th1 pathway. Significant difference (p value <0.05) with control group showed that CpG induces maturation after treatment with GM-CSF (without IL4). Discussion & Conclusions: Optimization of dendritic cell culture in terms of different culture conditions highly impacts the efficiency of immature cell formation and maturation.
{"title":"Optimized Mouse BMDC Isolation and Culture under \u0000Endotoxin-Free Conditions","authors":"Atefeh Sadeghi Shermeh, S. Habibzadeh, Adeleh Taghikhani, S. Rafati, N. Seyed","doi":"10.52547/sjimu.29.2.43","DOIUrl":"https://doi.org/10.52547/sjimu.29.2.43","url":null,"abstract":"Introduction: Dendritic cells are very important in basic studies and vaccine research, but isolation and culture of these cells face challenges due to their small number in tissues. Since there is no standard method, we addressed some of the factors affecting the efficiency of dendritic cell isolation and culture from BALB/c mouse bone marrow. Materials & Methods: Bone marrow cells isolated from femur and tibia, were seeded in plates and treated with GM-CSF. On day 3, half of the media was transferred to a new plate with fresh media plus GM-CSF. On day 5, cells were induced with CpG oligonucleotide. Cell survival as well as maturation markers of CD11C, MHC-II and CD86 were investigated by flow cytometry on day 5 (immature cells) and day 7 (mature cells). Ethics code: IR.PII.REC.1395.99 Findings: It was found that the presence of endotoxin in the culture of immature cells, significantly reduces the recovery of dendritic cells (p value <0.05). Less than one million cells seeded in non-treated 6-well plates and transfer of cells to a new plate on day 3 increased the efficiency of immature cell retrieval by preventing early maturation and premature death. Further treatment of immature cells with CpG on day 7 was used to induce the Th1 pathway. Significant difference (p value <0.05) with control group showed that CpG induces maturation after treatment with GM-CSF (without IL4). Discussion & Conclusions: Optimization of dendritic cell culture in terms of different culture conditions highly impacts the efficiency of immature cell formation and maturation.","PeriodicalId":16041,"journal":{"name":"journal of ilam university of medical sciences","volume":"115 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80866620","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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