Pub Date : 2019-01-01DOI: 10.5958/0973-9149.2019.00014.5
A. Baghel, D. Chhabra, R. Sharda, S. Audarya, R. Sikrodia
{"title":"Detection of Biofilm Forming Ability of Staphylococci Causing Bovine Mastitis Using Phenotypic and Genotypic Assays","authors":"A. Baghel, D. Chhabra, R. Sharda, S. Audarya, R. Sikrodia","doi":"10.5958/0973-9149.2019.00014.5","DOIUrl":"https://doi.org/10.5958/0973-9149.2019.00014.5","url":null,"abstract":"","PeriodicalId":16062,"journal":{"name":"Journal of Immunology and Immunopathology","volume":"18 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73232779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-01DOI: 10.5958/0973-9149.2019.00003.0
A. Dadawala, B. Mondal, H. N. Kher, H. Chauhan, A. Bhagat, M. D. Shrimali, B. Chandel
{"title":"Detection of Bluetongue Virus Antigen in Various Samples from Gujarat State, India","authors":"A. Dadawala, B. Mondal, H. N. Kher, H. Chauhan, A. Bhagat, M. D. Shrimali, B. Chandel","doi":"10.5958/0973-9149.2019.00003.0","DOIUrl":"https://doi.org/10.5958/0973-9149.2019.00003.0","url":null,"abstract":"","PeriodicalId":16062,"journal":{"name":"Journal of Immunology and Immunopathology","volume":"15 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89421409","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-01DOI: 10.5958/0973-9149.2019.00017.0
M. Shahzad, R. Garg, A. Devi, Shobha, F. Sheikh, H. Ram
{"title":"Molecular Detection of Babesia bigemina in Subclinically Infected Cattle","authors":"M. Shahzad, R. Garg, A. Devi, Shobha, F. Sheikh, H. Ram","doi":"10.5958/0973-9149.2019.00017.0","DOIUrl":"https://doi.org/10.5958/0973-9149.2019.00017.0","url":null,"abstract":"","PeriodicalId":16062,"journal":{"name":"Journal of Immunology and Immunopathology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90367117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-01DOI: 10.5958/0973-9149.2019.00001.7
O. R. V. Kumar, B. R. Singh, K. Sinha, Z. B. Dubal, B. S. Pruthvishree, Ramkumar N Rupner, Karthikeyan.
{"title":"Tackling Antimicrobial Resistance: Current Approaches","authors":"O. R. V. Kumar, B. R. Singh, K. Sinha, Z. B. Dubal, B. S. Pruthvishree, Ramkumar N Rupner, Karthikeyan.","doi":"10.5958/0973-9149.2019.00001.7","DOIUrl":"https://doi.org/10.5958/0973-9149.2019.00001.7","url":null,"abstract":"","PeriodicalId":16062,"journal":{"name":"Journal of Immunology and Immunopathology","volume":"14 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87193289","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-01DOI: 10.5958/0973-9149.2019.00018.2
V. Manjunatha, M. Rout, M. Krishna, N. Jaisingh, V. Srivastava, S. Byregowda
{"title":"Gastrointestinal Amebiasis Due to Entamoeba Histolytica in a Captive Male Zebra (Equus quagga)","authors":"V. Manjunatha, M. Rout, M. Krishna, N. Jaisingh, V. Srivastava, S. Byregowda","doi":"10.5958/0973-9149.2019.00018.2","DOIUrl":"https://doi.org/10.5958/0973-9149.2019.00018.2","url":null,"abstract":"","PeriodicalId":16062,"journal":{"name":"Journal of Immunology and Immunopathology","volume":"78 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82848063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-01DOI: 10.5958/0973-9149.2019.00004.2
V. Manjunatha, M. Rout, Umashankar, N. Salian, L. Kshama, S. Byregowda
Parasitic infections are widespread among reptiles and may cause serious problems. Any data on its prevalence may be useful for their health management in captivity. Hence, a survey through faecal sample examination was conducted to assess the helminth fauna in captive reptiles [Caiman crocodile (Caiman latirostris), Bengal monitor lizard (Varanus bengalensis) and Stared tortoise (Geochelone elegans)], maintained at Bannerghatta Biological Park, Bengaluru, Karnataka. A total of 23 faecal samples from apparently healthy Caiman crocodiles, 6 Bengal monitor lizards and 240 stared tortoises were collected over a period of 12 months during 2015–2016 and were screened using prescribed floatation and sedimentation technique followed by microscopic identification of eggs. We could detect ova of 6 (26.08%) Physaloptera sp. in Caiman crocodile, 3 (50%) Physaloptera sp. in Bengal monitor lizard and 157 (65.41%) oxyurid pinworms in stared tortoise. Upon necropsy, Physaloptera worms were recovered from the stomach of Bengal monitor lizard and pinworms from the stomach and intestine of stared tortoise. This study contributes to our knowledge on prevalence of parasites in the said reptiles in captivity.
{"title":"Survey of Helminth Parasites in Reptiles under Captivity","authors":"V. Manjunatha, M. Rout, Umashankar, N. Salian, L. Kshama, S. Byregowda","doi":"10.5958/0973-9149.2019.00004.2","DOIUrl":"https://doi.org/10.5958/0973-9149.2019.00004.2","url":null,"abstract":"Parasitic infections are widespread among reptiles and may cause serious problems. Any data on its prevalence may be useful for their health management in captivity. Hence, a survey through faecal sample examination was conducted to assess the helminth fauna in captive reptiles [Caiman crocodile (Caiman latirostris), Bengal monitor lizard (Varanus bengalensis) and Stared tortoise (Geochelone elegans)], maintained at Bannerghatta Biological Park, Bengaluru, Karnataka. A total of 23 faecal samples from apparently healthy Caiman crocodiles, 6 Bengal monitor lizards and 240 stared tortoises were collected over a period of 12 months during 2015–2016 and were screened using prescribed floatation and sedimentation technique followed by microscopic identification of eggs. We could detect ova of 6 (26.08%) Physaloptera sp. in Caiman crocodile, 3 (50%) Physaloptera sp. in Bengal monitor lizard and 157 (65.41%) oxyurid pinworms in stared tortoise. Upon necropsy, Physaloptera worms were recovered from the stomach of Bengal monitor lizard and pinworms from the stomach and intestine of stared tortoise. This study contributes to our knowledge on prevalence of parasites in the said reptiles in captivity.","PeriodicalId":16062,"journal":{"name":"Journal of Immunology and Immunopathology","volume":"60 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88622362","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-01DOI: 10.5958/0973-9149.2019.00006.6
H. Rashid, M. Batra, R. Chauhan
One hundred chickens of one day age were divided randomly into five groups of 20 birds each. After two weeks of adaptation period, group I was kept as control, group II was fed with 0.2% Spirulina platensis powder till 90th day post treatment (DPT). Group III was administered 1.6 ppm cadmium as cadmium chloride monohydrate (CdCl2.H2O) in triple glass distilled water. Groups IV and V were administered 1.61 ppm cadmium in triple glass distilled water along with Spirulina platensis powder orally in standard recommended feed at the rate of 0.2% concentration. However, in Group V Spirulina platensis powder was given after 60th day post treatment (DPT) till 90th day post treatment (DPT). Humoral immune response was assessed by hemagglutination and hemagglutination inhibition assays from the serum separated from 5 chickens from each group at 0, 30th, 60th and 90th DPT and cell mediated immune response was assessed using lymphocyte stimulation test from 5 chickens from each group at 0, 30th, 60th and 90th DPT using heparinised peripheral blood. There was a significant decrease in humoral immune response in cadmium treated group III, as compared to group I control. In groups IV and V, Spirulina platensis showed significant increase in humoral immune response in chickens which were also treated with cadmium. There was no significant change in cell mediated immune response in any group throughout the period of experiment. Present study showed the immunotoxic property of cadmium in chickens even at NOAEL dose and also Spirulina platensis has shown immunopotentiating effects in birds even in which the immunity was down regulated due to cadmium.
{"title":"Effect of Spirulina platensis on Immunity in Cadmium Treated Chickens","authors":"H. Rashid, M. Batra, R. Chauhan","doi":"10.5958/0973-9149.2019.00006.6","DOIUrl":"https://doi.org/10.5958/0973-9149.2019.00006.6","url":null,"abstract":"One hundred chickens of one day age were divided randomly into five groups of 20 birds each. After two weeks of adaptation period, group I was kept as control, group II was fed with 0.2% Spirulina platensis powder till 90th day post treatment (DPT). Group III was administered 1.6 ppm cadmium as cadmium chloride monohydrate (CdCl2.H2O) in triple glass distilled water. Groups IV and V were administered 1.61 ppm cadmium in triple glass distilled water along with Spirulina platensis powder orally in standard recommended feed at the rate of 0.2% concentration. However, in Group V Spirulina platensis powder was given after 60th day post treatment (DPT) till 90th day post treatment (DPT). Humoral immune response was assessed by hemagglutination and hemagglutination inhibition assays from the serum separated from 5 chickens from each group at 0, 30th, 60th and 90th DPT and cell mediated immune response was assessed using lymphocyte stimulation test from 5 chickens from each group at 0, 30th, 60th and 90th DPT using heparinised peripheral blood. There was a significant decrease in humoral immune response in cadmium treated group III, as compared to group I control. In groups IV and V, Spirulina platensis showed significant increase in humoral immune response in chickens which were also treated with cadmium. There was no significant change in cell mediated immune response in any group throughout the period of experiment. Present study showed the immunotoxic property of cadmium in chickens even at NOAEL dose and also Spirulina platensis has shown immunopotentiating effects in birds even in which the immunity was down regulated due to cadmium.","PeriodicalId":16062,"journal":{"name":"Journal of Immunology and Immunopathology","volume":"7 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88672008","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-01DOI: 10.5958/0973-9149.2019.00009.1
S. Sharma, R. Hazari
Mast cell tumors (MCTs) are very common in dogs, less common in cats, and uncommon to rare in other species. The biological behavior of MCT is highly variable; some tumors are benign whereas others have aggressive growth and metastasize. Recent studies revealed the role of stem cell receptor (KIT) in the etiology of canine MCTs. KIT is normally expressed on hematopoietic cells and mast cells. Present communication describes a mast cell tumour with fast growing and recurring nature. Histopathologically tumour comprises of diffuse sheets of uniform dyscohesive round cells separated by incomplete fibrous septae. These cells have scant to moderate cytoplasm and round to oval nucleus with slightly opened up chromatin. Immunohistochemically diffuse patterns of KIT expression were seen in tumour cells with granular labeling of mast cells.
{"title":"Cutaneous Mast Cell Tumour in Dog: A Case Study","authors":"S. Sharma, R. Hazari","doi":"10.5958/0973-9149.2019.00009.1","DOIUrl":"https://doi.org/10.5958/0973-9149.2019.00009.1","url":null,"abstract":"Mast cell tumors (MCTs) are very common in dogs, less common in cats, and uncommon to rare in other species. The biological behavior of MCT is highly variable; some tumors are benign whereas others have aggressive growth and metastasize. Recent studies revealed the role of stem cell receptor (KIT) in the etiology of canine MCTs. KIT is normally expressed on hematopoietic cells and mast cells. Present communication describes a mast cell tumour with fast growing and recurring nature. Histopathologically tumour comprises of diffuse sheets of uniform dyscohesive round cells separated by incomplete fibrous septae. These cells have scant to moderate cytoplasm and round to oval nucleus with slightly opened up chromatin. Immunohistochemically diffuse patterns of KIT expression were seen in tumour cells with granular labeling of mast cells.","PeriodicalId":16062,"journal":{"name":"Journal of Immunology and Immunopathology","volume":"19 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87117813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-01DOI: 10.5958/0973-9149.2019.00016.9
G. Sawale, M. Lakshman, N. Bulbule, R. Pardeshi, S. Raut, M. Chawak, Y. Ravikumar, D. Madhuri
{"title":"Effect of Avian Reovirus Infection on Antibody Response, Footpad Thickness and Body Weight in SPF Chicken","authors":"G. Sawale, M. Lakshman, N. Bulbule, R. Pardeshi, S. Raut, M. Chawak, Y. Ravikumar, D. Madhuri","doi":"10.5958/0973-9149.2019.00016.9","DOIUrl":"https://doi.org/10.5958/0973-9149.2019.00016.9","url":null,"abstract":"","PeriodicalId":16062,"journal":{"name":"Journal of Immunology and Immunopathology","volume":"26 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73462261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-01DOI: 10.5958/0973-9149.2019.00007.8
K. Karthik, M. Mashooq, V. Nagaleekar
Clostridium chauvoei, the causative of black quarter in ruminants, is an anaerobic spore forming organism, produces different types of exotoxins. Clostridium chauvoei toxin A (cctA) is an important toxin possessing hemolytic and cytotoxic property and also claimed to be a protective antigen. Characterization of the different regions of the toxin has not been carried out yet. Hence, the regions responsible for different activities of the toxin are not clear. As an initial step, amino-terminal region (34-75aa) of cctA gene was cloned and expressed in pRham-N-His-Sumo-Kan vector. Expressed protein was confirmed by SDS-PAGE analysis.
chauvoei梭状芽胞杆菌是一种厌氧孢子形成的生物，是反刍动物黑斑病的病原体，产生不同类型的外毒素。chauvoei Clostridium toxin A (cctA)是一种重要的毒素，具有溶血和细胞毒性，被认为是一种保护性抗原。目前还没有对毒素的不同区域进行表征。因此，负责毒素不同活动的区域并不清楚。首先克隆cctA基因氨基末端区(34-75aa)，并在pRham-N-His-Sumo-Kan载体中表达。通过SDS-PAGE分析证实表达蛋白。
{"title":"Expression of Amino-terminal Fragment of Clostridium chauvoei toxin A","authors":"K. Karthik, M. Mashooq, V. Nagaleekar","doi":"10.5958/0973-9149.2019.00007.8","DOIUrl":"https://doi.org/10.5958/0973-9149.2019.00007.8","url":null,"abstract":"Clostridium chauvoei, the causative of black quarter in ruminants, is an anaerobic spore forming organism, produces different types of exotoxins. Clostridium chauvoei toxin A (cctA) is an important toxin possessing hemolytic and cytotoxic property and also claimed to be a protective antigen. Characterization of the different regions of the toxin has not been carried out yet. Hence, the regions responsible for different activities of the toxin are not clear. As an initial step, amino-terminal region (34-75aa) of cctA gene was cloned and expressed in pRham-N-His-Sumo-Kan vector. Expressed protein was confirmed by SDS-PAGE analysis.","PeriodicalId":16062,"journal":{"name":"Journal of Immunology and Immunopathology","volume":"19 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85152168","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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