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Assessing Radiosensitivity: Effects of Acute Ionizing Radiation on Inflammation and Apoptosis in Macrophage Cell Line (RAW 264.7) 评估放射敏感性:急性电离辐射对巨噬细胞炎症和凋亡的影响(RAW 264.7)
Q2 Medicine Pub Date : 2023-10-09 DOI: 10.34172/jlms.2023.40
Zahra Bagheri-Hosseinabadi, Jaber Zafari, Fatemeh Javani Jouni, Hanieh Sadeghi, Mitra Abbasifard
Introduction: The responses of biological systems to various types of radiation have multifaceted dimensions. In the field of ionizing radiation, in vitro external gamma radiation therapy has primarily been studied as a model to elucidate the challenges that biological systems face from radiation effects. Exposure of cells/organisms to gamma radiation results in a cascade of ionization events that can cause severe and irreversible biological damage. However, the biological responses and oxidative stress-related mechanisms under acute radiation conditions remain poorly understood in inflammatory systems. The present study aimed to provide a model of the effect of ionizing radiation on macrophages, which play a pivotal role in the mechanisms of inflammation, to assess the impact of radiotherapy as an approach to treating inflammatory diseases. Methods: A macrophage cell line (RAW 264.7) was cultured and exposed to different doses of gamma radiation (4, 6, 8, 10 Gy). Cell viability, apoptosis, cell cycle, migration, nitric oxide (NO) and prostaglandin E2 (PGE2) production, expression of pro-inflammatory and apoptotic genes, and cytokine secretion of macrophages were also evaluated. Results: The results showed that gamma radiation at 4 Gy had a low effect on macrophage characteristics and cytokine secretion patterns. In contrast, higher doses (8 and 10 Gy) increased DNA damage, expression of apoptotic genes, and secretion of NO and PGE2 cytokines. 6 Gy radiation, the maximum radiation dose, showed moderate non-destructive effects and inflammation process modulation. In this study, doses higher than 6 Gy of Gamma radiation caused cell mortality. Conclusion: It appears that 6 Gy of gamma radiation modulates the inflammatory cascade caused by macrophage cells.
生物系统对各种类型的辐射的反应是多方面的。在电离辐射领域,体外γ射线治疗主要是作为一个模型来研究,以阐明生物系统面临的辐射效应的挑战。细胞/生物体暴露于伽马辐射会导致一系列电离事件,可造成严重和不可逆转的生物损伤。然而,炎症系统在急性辐射条件下的生物反应和氧化应激相关机制仍然知之甚少。巨噬细胞在炎症机制中起着关键作用,本研究旨在建立电离辐射对巨噬细胞影响的模型,以评估放射治疗作为治疗炎症性疾病的方法的影响。方法:培养巨噬细胞(RAW 264.7),并暴露于不同剂量的γ辐射(4,6,8,10 Gy)。观察巨噬细胞的细胞活力、凋亡、细胞周期、迁移、一氧化氮(NO)和前列腺素E2 (PGE2)的产生、促炎和凋亡基因的表达以及细胞因子的分泌。结果:4 Gy γ辐射对巨噬细胞特性和细胞因子分泌模式的影响较小。相反,较高剂量(8和10 Gy)增加了DNA损伤、凋亡基因的表达以及NO和PGE2细胞因子的分泌。最大辐射剂量为6gy,表现出中等的非破坏性作用和炎症过程调节。在这项研究中,高于6戈瑞的伽马辐射剂量导致细胞死亡。结论:6 Gy γ辐射可调节巨噬细胞引起的炎症级联反应。
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引用次数: 0
Capillary Hemangioma Managed With Laser Ablation: A Case Report 激光消融治疗毛细血管瘤1例
Q2 Medicine Pub Date : 2023-09-30 DOI: 10.34172/jlms.2023.39
Aravind Warrier S, Vaishnavi Muthukumaran, Aparna Venkatramakrishnan, Divyambika CV, Thamizhchelvan H, Muthukumar Santhanakrishnan
Introduction: Hemangiomas are hamartomatous benign tumours of the vascular tissue, which are common among infants and adolescents and are found in the head and neck region. Oral hemangiomas are infrequently encountered in the palate. Baseline soft tissue imaging must be considered for an appropriate diagnosis and treatment approach. Case Report: A 68-year-old female presented with a painless growth in the roof of her mouth that gradually increased in its size. She was subjected to intraoral ultrasonography and was diagnosed with capillary hemangioma. In this instance, laser ablation was chosen as the preferred treatment due to the complications associated with the conventional surgery. Conclusion: This case report enlightens the systematic diagnostic approach and treatment plan.
简介:血管瘤是血管性组织的错构瘤性良性肿瘤,常见于婴儿和青少年,多见于头颈部。口腔血管瘤很少发生在上颚。基线软组织成像必须考虑一个适当的诊断和治疗方法。病例报告:一名68岁女性,她的上颚无痛性生长并逐渐增大。经口内超声检查,诊断为毛细血管瘤。在本例中,由于常规手术的并发症,选择激光消融作为首选治疗方法。结论:本病例报告对系统的诊断方法和治疗方案有所启发。
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引用次数: 0
Impact of Er,Cr:YSGG Laser, Sandblast, and Acid Etching Surface Modification on Surface Topography of Biodental Titanium Implants Er、Cr:YSGG激光、喷砂和酸蚀表面改性对生物牙钛种植体表面形貌的影响
Q2 Medicine Pub Date : 2023-09-30 DOI: 10.34172/jlms.2023.38
Mohammed A Abdulla, Radhwan H Hasan, Osama Hazim Al-Hyani
Introduction: Several techniques have been used to modify the surface of commercially pure titanium (CPTi) implants to improve osseointegration using lasers, sandblasts, sandblasts with acid etching, and other modalities. For implant-osseointegration, surface features like chemical composition of a surface, topography, and surface energy are essential. The present comparative study aimed to compare the impact of Er,Cr:YSGG laser, sandblasting, and acid etching implant surface modifications on the surface topography, roughness, and element chemical composition of the Ti dental implant. Methods: Thirty CPTi dental implants were divided into three groups according to the surface modification (n=10 for each group): Group A: Sandblasting with acid etching (SLActive), group B: Sandblasting, and group C: Er,Cr:YSGG laser surface modifications. The modified surfaces were analyzed using scanning electron microscopy (SEM), profilometer, and energy dispersive x-ray spectrometry (EDS). Results: One-way analysis of variance (ANOVA) showed that there were significant differences in the mean values of average roughness (Ra) of the three groups (P<0.05). Tukey’s post hoc test showed that the average roughness (Ra) of laser-surface modification (group C) of the implant had the highest mean value (2.30 µm) among the different groups, while sandblasted surface modification (group B) of the implant had the lowest mean value (1.39 µm). The SLActive (group A) sandblast with acid etching had a mean value of 1.63 µm. SEM analysis showed that significantly modified surface topographies and different element concentrations were found within all modified groups. Conclusion: The Er,Cr:YSGG laser irradiation increased the implant surface roughness value after implant surface modification, compared to sandblasts and sandblasts with acid etching application. The observations for the SEM-EDS analysis revealed several elements with different concentrations, which were affected by the physical–chemical characteristics of the surface modification techniques. The SEM analysis showed a significant modification in implant surface topographies of the tested groups.
导读:目前已经有几种技术被用来修饰商业纯钛(CPTi)植入物的表面,以改善骨整合,包括激光、喷砂、酸蚀喷砂和其他方式。对于种植体-骨整合,表面特征,如表面的化学成分,地形和表面能是必不可少的。本对比研究旨在比较Er、Cr:YSGG激光、喷砂和酸蚀种植体表面修饰对钛牙种植体表面形貌、粗糙度和元素化学成分的影响。方法:将30颗CPTi种植体按表面修饰情况分为3组(每组10颗):A组:酸蚀喷砂(SLActive)组,B组:喷砂组,C组:Er,Cr:YSGG激光表面修饰组。利用扫描电镜(SEM)、轮廓仪(profilometer)和能量色散x射线能谱仪(EDS)对改性后的表面进行分析。结果:单因素方差分析(ANOVA)显示,三组平均粗糙度(Ra)的平均值差异有统计学意义(P<0.05)。Tukey’s事后检验显示,激光表面修饰组(C组)的平均粗糙度Ra在不同组中平均值最高(2.30µm),喷砂表面修饰组(B组)的平均值最低(1.39µm)。酸蚀SLActive (A组)喷砂的平均值为1.63µm。扫描电镜分析表明,在所有修饰组中,表面形貌和元素浓度都发生了显著变化。结论:与喷砂和酸蚀喷砂相比,Er,Cr:YSGG激光照射提高了种植体表面改性后的表面粗糙度值。SEM-EDS分析表明,不同表面改性工艺的物理化学特性会影响到不同浓度的元素。扫描电镜分析显示,试验组种植体表面形貌有明显改变。
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引用次数: 0
Analysis of the Temperature Elevation of the Dual-Wavelength Diode Laser and the Er, Cr: YSGG Laser in Oral Soft Tissue Incisions 双波长二极管激光与Er, Cr: YSGG激光在口腔软组织切口中的温升分析
Q2 Medicine Pub Date : 2023-09-25 DOI: 10.34172/jlms.2023.37
Alaa Jamal Al-Ani, Ammar Saleh Al-Alawi, Hanan Jafer Taher
Introduction: Lasers are one of the most advantageous tools that have been used in a variety of medical fields. Soft tissue management is an important part of oral and maxillofacial surgery. There are different surgical procedures for management; one of them is laser surgery. The purpose of this study was to compare the dual-wavelength diode laser and the Er, Cr: YSGG laser to choose the most effective laser for a soft tissue incision in oral and maxillofacial surgery regarding temperature elevation. Methods: A dual-wavelength diode laser (810 and 980 nm) with 1.5 W and 2.5 W power outputs and an Er, Cr: YSGG (2780 nm) with 2.5 W and 3.5 W power outputs were used to make 50 incisions in six freshly dissected sheep tongues. The temperature means were measured at initial, maximum temperatures, as well as the temperature rise (difference between initial and maximum), and all were compared between the groups of soft tissue. Results: The lowest mean temperature rise was observed with a 2.5 W Er, Cr: YSGG laser, and the highest mean temperature rise was observed with a 2.5 W diode laser, and all samples from the two lasers showed a statistically significant difference (P<0.05) in the temperature rise except the 1.5 W diode, which is higher but not statistically significantly different from the 3.5 W Er, Cr: YSGG laser (P=0.100). Conclusion: The power output of 2.5 W of an Er, Cr: YSGG laser produced low levels of temperature rise compared to a diode laser and produced surgical incisions with a lower probability of heat damage to surrounding tissues.
激光是最有利的工具之一,已被用于各种医疗领域。软组织处理是口腔颌面外科的重要组成部分。有不同的外科治疗方法;其中之一就是激光手术。本研究的目的是比较双波长二极管激光和Er, Cr: YSGG激光,以选择最有效的激光用于口腔颌面外科软组织切口的温度升高。方法:采用功率分别为1.5 W和2.5 W的双波长二极管激光器(810和980 nm)和功率分别为2.5 W和3.5 W的Er, Cr: YSGG激光器(2780 nm)在6只新鲜解剖的羊舌上切开50个切口。测量初始温度、最高温度、温升(初始与最高温度之差)的温度均值,并比较各组软组织间的温度均值。结果:2.5 W Er, Cr: YSGG激光器的平均温升最低,2.5 W二极管激光器的平均温升最高,除1.5 W二极管激光器的温升高于3.5 W Er, Cr: YSGG激光器外,两种激光器样品的温升差异均有统计学意义(P= 0.05),但差异无统计学意义(P=0.100)。结论:与二极管激光器相比,功率为2.5 W的Er, Cr: YSGG激光器产生的温升水平较低,手术切口对周围组织的热损伤概率较低。
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引用次数: 0
Therapeutic Effects of Low-Level Laser on Male Infertility: A Systematic Review 低水平激光治疗男性不育症的疗效综述
Q2 Medicine Pub Date : 2023-09-25 DOI: 10.34172/jlms.2023.36
Amirreza Eghbaldoost, Seyed Pooria Salehi Mashhadsari, Erfan Ghadirzadeh, Alireza Ghoreifi, Farzad Allameh
Introduction: The objective of this study was to assess the effectiveness and safety of photobiomodulation (PBM) in the treatment of male infertility. Methods: We searched Google Scholar, PubMed, and the reference sections of relevant papers published from January 1, 2000 to September 23, 2022. We retrieved all publications related to the impact of PBM on male infertility. After reviewing the titles, abstracts, and full texts, we included fifteen papers in the research. The studies involved 477 semen samples (in vitro studies) and 70 male participants (randomized clinical trials). Results: All 14 in vitro studies that evaluated effectiveness reported that PBM was successful in increasing the proportion of progressive sperms in semen samples. Various methods were used to evaluate the safety. One study with a sample size of 58 concluded that PBM was not a safe treatment, whereas the other ten studies confirmed its safety. Only one clinical trial evaluated the effect of laser acupuncture on male infertility and found improvements in sperm progressive motility without any serious adverse effects. Conclusion: All 15 studies evaluating effectiveness reported that the low-level laser was effective for increasing the proportion of progressive sperm in semen samples and that it was safe to use. However, due to the heterogeneity of population characteristics, source characteristics, duration of exposure, sample size, and instruments for measuring safety and efficacy, we cannot conclude that the positive results obtained from the reviewed studies are solely attributable to the low-level laser on the sperm samples.
前言:本研究的目的是评估光生物调节(PBM)治疗男性不育症的有效性和安全性。方法:检索2000年1月1日至2022年9月23日发表的相关论文的Google Scholar、PubMed和参考文献部分。我们检索了所有与PBM对男性不育影响相关的出版物。在审阅了题目、摘要和全文后,我们纳入了15篇论文。这些研究涉及477份精液样本(体外研究)和70名男性参与者(随机临床试验)。结果:所有14项评估有效性的体外研究报告称,PBM成功地增加了精液样本中进展精子的比例。采用多种方法评价其安全性。一项有58个样本量的研究得出结论,PBM不是一种安全的治疗方法,而其他10项研究证实了它的安全性。只有一项临床试验评估了激光针灸对男性不育症的影响,发现精子进行性运动的改善没有任何严重的不良反应。结论:所有15项评估有效性的研究都报告了低水平激光对增加精液样本中进展性精子的比例是有效的,并且是安全的。然而,由于人群特征、源特征、暴露时间、样本量以及测量安全性和有效性的仪器的异质性,我们不能得出结论,从所审查的研究中获得的阳性结果完全归因于低水平激光对精子样本的影响。
{"title":"Therapeutic Effects of Low-Level Laser on Male Infertility: A Systematic Review","authors":"Amirreza Eghbaldoost, Seyed Pooria Salehi Mashhadsari, Erfan Ghadirzadeh, Alireza Ghoreifi, Farzad Allameh","doi":"10.34172/jlms.2023.36","DOIUrl":"https://doi.org/10.34172/jlms.2023.36","url":null,"abstract":"Introduction: The objective of this study was to assess the effectiveness and safety of photobiomodulation (PBM) in the treatment of male infertility. Methods: We searched Google Scholar, PubMed, and the reference sections of relevant papers published from January 1, 2000 to September 23, 2022. We retrieved all publications related to the impact of PBM on male infertility. After reviewing the titles, abstracts, and full texts, we included fifteen papers in the research. The studies involved 477 semen samples (in vitro studies) and 70 male participants (randomized clinical trials). Results: All 14 in vitro studies that evaluated effectiveness reported that PBM was successful in increasing the proportion of progressive sperms in semen samples. Various methods were used to evaluate the safety. One study with a sample size of 58 concluded that PBM was not a safe treatment, whereas the other ten studies confirmed its safety. Only one clinical trial evaluated the effect of laser acupuncture on male infertility and found improvements in sperm progressive motility without any serious adverse effects. Conclusion: All 15 studies evaluating effectiveness reported that the low-level laser was effective for increasing the proportion of progressive sperm in semen samples and that it was safe to use. However, due to the heterogeneity of population characteristics, source characteristics, duration of exposure, sample size, and instruments for measuring safety and efficacy, we cannot conclude that the positive results obtained from the reviewed studies are solely attributable to the low-level laser on the sperm samples.","PeriodicalId":16224,"journal":{"name":"Journal of lasers in medical sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135867125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effect of the 1064 nm Nd: YAG Laser on the MICs of Antifungals Used in Clinical Practice for the Treatment of Fungal Nail Infections 1064 nm Nd: YAG激光对临床治疗真菌指甲感染抗真菌药物mic的影响
Q2 Medicine Pub Date : 2023-09-24 DOI: 10.34172/jlms.2023.35
Taraneh Razavyoon, Seyed Jamal Hashemi, Parvin Mansouri, Roshanak Daie Ghazvini, Sadegh Khodavaisy, Heydar Bakhshi, Saham Ansari, Zahra Rafat, Nahid Nikkhah, Bahram Mohajer, Shayesteh Razavyoon, Davoud Roostaei
Introduction: The fungal nail infection (onychomycosis) involves 18%-40% of all nail disorders, which, although not fatal, can cause mechanical, aesthetic, occupational, and economic problems. Drug treatments due to prolonged treatment periods, drug interactions, adverse effects, and slow progression may associate with numerous negative outcomes. This study aimed to evaluate the long-pulsed 1064-nm Nd: YAG laser effect on fungal colonies and subsequently possible change in the minimum inhibitory concentrations (MICs) of common antifungals compared with the same non-lasered colonies as a novel way to investigate laser and antifungal interaction. Methods: Sixty onychomycosis samples consisting of saprophyte (n=20), dermatophyte (n=20), and yeast (n=20) duplicate colonies were isolated. A series was treated by a long-pulsed 1064-nm Nd: YAG laser. Afterward, the MIC (CLSI-M38-A2 and CLSI-M27-A3) of two series against common antifungals were compared. Results: After 1064-nm Nd: YAG laser irradiation in all 20 tested saprophytes, the MICs of terbinafine (P value<0.035) were changed, and in all 20 tested dermatophytes, the MICs of voriconazole (P value<0.021) were changed. Also, in all 20 tested yeasts, the MICs of caspofungin (P value<0.037) were changed. Moreover, in saprophytes, dermatophytes, and yeasts, significant changes in the MICs of itraconazole (P value<0.032), terbinafine (P value<0.025), and caspofungin (P value<0.037) were detected. Our result showed the GM MICs of the 1064-nm Nd: YAG laser in all saprophyte, dermatophyte, and yeast groups were lower than in the control group. Conclusion: The present study indicated that the long-pulsed 1064-nm Nd: YAG laser significantly changes the MICs of antifungals in onychomycosis clinical samples.
指甲真菌感染(甲真菌病)占所有指甲疾病的18%-40%,虽然不是致命的,但可以引起机械、美学、职业和经济问题。药物治疗由于治疗时间延长、药物相互作用、不良反应和缓慢的进展可能与许多负面结果相关。本研究旨在评估长脉冲1064 nm Nd: YAG激光对真菌菌落的影响,以及与相同的非激光菌落相比,常见抗真菌药物的最低抑制浓度(mic)可能发生的变化,作为研究激光与抗真菌相互作用的新方法。方法:对60份甲真菌标本进行分离,其中包括腐生菌(n=20)、皮肤菌(n=20)和酵母菌(n=20)重复菌落。采用长脉冲1064 nm Nd: YAG激光处理。然后比较两个系列对常见抗真菌药物的MIC (CLSI-M38-A2和CLSI-M27-A3)。结果:1064 nm Nd: YAG激光照射20种腐生植物后,特比萘芬的mic均发生变化(P值为0.035),伏立康唑的mic均发生变化(P值为0.021)。此外,在所有20个被试酵母中,caspofungin的mic (P值<0.037)都发生了变化。此外,在腐生植物、皮肤植物和酵母中,检测到伊曲康唑(P值<0.032)、特比萘芬(P值<0.025)和卡泊芬素(P值<0.037)的mic有显著变化。结果表明,1064 nm Nd: YAG激光在腐生植物、皮肤植物和酵母菌组的转基因mic均低于对照组。结论:长脉冲1064 nm Nd: YAG激光可显著改变甲癣临床样品中抗真菌药物的mic。
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引用次数: 0
Evaluation of the Effects of Photodynamic Therapy With Methylene Blue on Different Candida Species In Vitro 亚甲基蓝光动力疗法对不同假丝酵母菌体外治疗效果的评价
Q2 Medicine Pub Date : 2023-09-18 DOI: 10.34172/jlms.2023.34
Faezeh Khozeimeh, Atefeh Tavangar, Mehdi Razaghi Abyaneh, Milad Amini, Marzieh Ghayour
Introduction: Oral candidiasis is the most prevalent opportunistic infection of the oral cavity. The most common cause of this infection is Candida albicans. Considering the side effects of conventional antifungal therapies, this study aimed to evaluate the efficacy of photodynamic therapy with new methylene blue (a photosensitizer) in inhibiting the growth of C. albicans, C. tropicalis, C. glabrata, and C. krusei in vitro. Methods: In this experimental study, 200 samples of standard suspension (0.5 McFarland) were prepared from C. albicans, C. tropicalis, C. glabrata, and C. krusei (50 samples from each species). The samples of each species were divided into five groups (n=10), including photodynamic therapy with a photosensitizer, with or without laser irradiation, nystatin treatment, laser therapy, and control. Next, cultivation of samples was performed on Sabouraud dextrose agar, and the colony-forming units were determined after 24 hours of incubation at 37 °C. Data were analyzed in SPSS version 22 by means of the Kruskal-Wallis test (P<0.05). Results: The most sensitive and resistant species to nystatin therapy were C. glabrata and C. krusei, respectively. On the other hand, C. krusei was the most sensitive species to photodynamic therapy, and C. glabrata was the most resistant type to this treatment. The highest therapeutic effectiveness was attributed to nystatin therapy, although photodynamic therapy was also effective. Laser therapy was recognized as the least effective method. Conclusion: Photodynamic therapy with new methylene blue, as a suitable adjunct therapy, can be effective in the management of candidiasis. It may also be a potential novel treatment for immunocompromised patients with oral candidiasis.
口腔念珠菌病是口腔最常见的机会性感染。这种感染最常见的原因是白色念珠菌。考虑到常规抗真菌疗法的副作用,本研究旨在评估新型亚甲基蓝(光敏剂)光动力疗法在体外抑制白色念珠菌、热带念珠菌、光斑念珠菌和克鲁塞念珠菌生长的效果。方法:以白色念珠菌、热带念珠菌、光斑念珠菌和克鲁塞念珠菌各50份,制备标准悬浮液200份(0.5 McFarland)。每个物种的标本分为5组(n=10),包括光敏剂光动力治疗组、激光照射组、制霉菌素治疗组、激光治疗组和对照组。接下来,在Sabouraud葡萄糖琼脂上进行样品培养,37℃孵育24小时后测定菌落形成单位。数据在SPSS version 22中进行分析,采用Kruskal-Wallis检验(P<0.05)。结果:对制霉菌素治疗最敏感和最耐药的菌种分别为光秃秃夜蛾和克氏夜蛾。另一方面,对光动力处理最敏感的品种是克鲁西,对光动力处理最抗性的品种是光动力处理。最高的治疗效果归因于制霉菌素治疗,尽管光动力治疗也有效。激光治疗被认为是最不有效的方法。结论:新亚甲蓝光动力疗法是治疗念珠菌病的一种合适的辅助疗法。它也可能是一种潜在的治疗口腔念珠菌病免疫功能低下患者的新方法。
{"title":"Evaluation of the Effects of Photodynamic Therapy With Methylene Blue on Different <i>Candida</i> Species <i>In Vitro</i>","authors":"Faezeh Khozeimeh, Atefeh Tavangar, Mehdi Razaghi Abyaneh, Milad Amini, Marzieh Ghayour","doi":"10.34172/jlms.2023.34","DOIUrl":"https://doi.org/10.34172/jlms.2023.34","url":null,"abstract":"Introduction: Oral candidiasis is the most prevalent opportunistic infection of the oral cavity. The most common cause of this infection is Candida albicans. Considering the side effects of conventional antifungal therapies, this study aimed to evaluate the efficacy of photodynamic therapy with new methylene blue (a photosensitizer) in inhibiting the growth of C. albicans, C. tropicalis, C. glabrata, and C. krusei in vitro. Methods: In this experimental study, 200 samples of standard suspension (0.5 McFarland) were prepared from C. albicans, C. tropicalis, C. glabrata, and C. krusei (50 samples from each species). The samples of each species were divided into five groups (n=10), including photodynamic therapy with a photosensitizer, with or without laser irradiation, nystatin treatment, laser therapy, and control. Next, cultivation of samples was performed on Sabouraud dextrose agar, and the colony-forming units were determined after 24 hours of incubation at 37 °C. Data were analyzed in SPSS version 22 by means of the Kruskal-Wallis test (P<0.05). Results: The most sensitive and resistant species to nystatin therapy were C. glabrata and C. krusei, respectively. On the other hand, C. krusei was the most sensitive species to photodynamic therapy, and C. glabrata was the most resistant type to this treatment. The highest therapeutic effectiveness was attributed to nystatin therapy, although photodynamic therapy was also effective. Laser therapy was recognized as the least effective method. Conclusion: Photodynamic therapy with new methylene blue, as a suitable adjunct therapy, can be effective in the management of candidiasis. It may also be a potential novel treatment for immunocompromised patients with oral candidiasis.","PeriodicalId":16224,"journal":{"name":"Journal of lasers in medical sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135256550","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effect of Photodynamic Therapy on the Pushout Bond Strength of Resin-Based and Calcium Silicate-Based Endodontic Sealers 光动力疗法对树脂基和硅酸钙基根管密封剂推出结合强度的影响
Q2 Medicine Pub Date : 2023-09-10 DOI: 10.34172/jlms.2023.33
Saeede Zadsirjan, Mohammad Asnaashari, Atoosa Yazdani, Soolmaz Heidari, Tina Estarami
Introduction: This study aimed to assess the effect of photodynamic therapy (PDT) on the pushout bond strength (PBS) of resin-based and calcium silicate-based endodontic sealers. Methods: Forty single-canal teeth were assigned to four groups. After root canal preparation by the crown-down technique and smear layer removal, groups 1 and 2 served as the control group, and groups 3 and 4 underwent PDT by using a low-level diode laser as the light source and methylene blue as the photosensitizer (MB). The root canals in groups 1 and 3 were filled with AH26 sealer while the root canals in groups 2 and 4 were filled with Endoseal TCS. The samples were sectioned at 4, 8, and 12 mm from the apex tip (1mm thick slices). The PBS test was performed, and the mode of failure was determined in the coronal, middle, and apical thirds. The data were analyzed by the Kruskal-Wallis, Friedman, and Dunn tests. Results: No significant difference was noted in the PBS of resin-based and calcium silicate-based sealers in the apical, middle, or coronal thirds in the application of PDT (P>0.05). The PBS of AH26 was significantly higher than that of Endoseal TCS (P<0.05). Cohesive failure was the dominant mode of failure in the AH26 groups, while mixed failure was the dominant failure mode in the Endoseal TCS groups. Conclusion: PDT had no significant effect on the PBS of resin-based and calcium silicate-based sealers.
简介:本研究旨在评估光动力疗法(PDT)对树脂基和硅酸钙基根管密封剂的推出结合强度(PBS)的影响。方法:40颗单管牙分为4组。采用冠下法预备根管并去除涂片层后,1、2组作为对照组,3、4组以低能级二极管激光为光源,亚甲基蓝为光敏剂(MB)进行PDT治疗。1、3组用AH26密封剂填充根管,2、4组用Endoseal TCS填充根管。样品在距离尖端4、8和12 mm处切片(1mm厚的切片)。进行PBS试验,确定冠状、中间和根尖三分之一的失效模式。数据通过Kruskal-Wallis, Friedman和Dunn测试进行分析。结果:树脂基和硅酸钙基封口剂在根尖、中、冠状三分之一的PBS应用PDT时,差异无统计学意义(P>0.05)。AH26的PBS显著高于Endoseal TCS (p < 0.05)。AH26组以内聚性失效为主,Endoseal TCS组以混合性失效为主。结论:PDT对树脂基和硅酸钙基密封剂的PBS无显著影响。
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引用次数: 0
The Effect of Radiofrequency Therapy on Sexual Function in Female Cancer Survivors (Gynecologic and Breast) and Non-cancer Menopausal Women: A Single-Arm Trial. 射频治疗对女性癌症幸存者(妇科和乳腺)和非癌症更年期妇女性功能的影响:一项单臂试验。
IF 1.5 Q2 Medicine Pub Date : 2023-09-02 eCollection Date: 2023-01-01 DOI: 10.34172/jlms.2023.32
Maliheh Arab, Mahshid Vasef, Maryam Talayeh, Maryam Sadat Hosseini, Farah Farzaneh, Tahereh Ashrafganjoei

Introduction: Up to 90% of postmenopausal women and female cancer survivors may be affected by the genitourinary syndrome of menopause (GSM), with a negative impact on sexual function and quality of life. A novel energy-based device among the treatment options for GSM is radiofrequency therapy (RFT). RFT is a treatment option that uses energy from radio waves to heat the tissue. The objective of this study was to assess the impact of RFT on sexual function in female cancer survivors (gynecologic and breast) and non-cancer menopausal women. Methods: In a single-arm prospective trial, the efficacy of RFT in both female cancer survivors (gynecologic and breast) and non-cancer menopausal women with sexual dysfunction at a tertiary and referral center (Imam Hossein Medical Center, Tehran, Iran) was evaluated between April 2022 and December 2022. The study protocol consisted of 3 monthly RFT sessions. Examination was performed at baseline (T0) and 3 months after the last RFT session (T1). The primary outcome was sexual function, which was assessed using the Female Sexual Function Index (FSFI). In addition, adverse events were evaluated during treatment and at T1. Results: A total of 37 female cancer survivors (mean [SD] age: 49.4 [8.9] years) and 37 non-cancer menopausal women (mean [SD] age: 53.8 [5.5] years) were enrolled. Patients exposed to RFT showed a significant improvement in FSFI scores when compared to baseline scores for both female cancer survivors (13.07, 95% CI: 12.27 - 13.86) and non-cancer menopausal women (13.18, 95% CI: 12.34 - 14.03). There was no difference in FSFI total score improvement between the two groups (t(72)=0.06, P=0.951). There were no serious adverse events associated with RFT. Conclusion: The efficacy of RFT as a treatment for sexual dysfunction has been demonstrated in both non-cancer menopausal women and female cancer survivors. In both groups, a significant improvement was confirmed.

简介:高达90%的绝经后妇女和癌症女性幸存者可能受到更年期泌尿生殖综合征(GSM)的影响,对性功能和生活质量产生负面影响。在GSM的治疗选择中,一种新型的基于能量的设备是射频治疗(RFT)。RFT是一种利用无线电波能量加热组织的治疗选择。本研究的目的是评估RFT对女性癌症幸存者(妇科和乳腺)和非癌症更年期妇女性功能的影响。方法:在一项单臂前瞻性试验中,评估了RFT在2022年4月至2022年12月期间在三级和转诊中心(伊朗德黑兰Imam Hossein医疗中心)对癌症女性幸存者(妇科和乳腺)和性功能障碍的非癌症更年期妇女的疗效。研究方案包括3个月的RFT会议。在基线(T0)和最后一次RFT治疗后3个月(T1)进行检查。主要结果是性功能,使用女性性功能指数(FSFI)进行评估。此外,在治疗期间和T1时对不良事件进行了评估。结果:共有37名癌症女性幸存者(平均[SD]年龄:49.4[8.9]岁)和37名非癌症更年期女性(平均[SS]年龄:53.8[5.5]岁)入选。与癌症女性幸存者(13.07,95%CI:12.27-13.86)和非癌症更年期女性(13.18,95%CI:12.34-14.03)的基线评分相比,接受RFT的患者的FSFI评分显著改善。两组的FSFI总分改善无差异(t(72)=0.06,P=0.951)。没有与RFT相关的严重不良事件。结论:RFT作为治疗性功能障碍的有效性已在非癌症更年期妇女和女性癌症幸存者中得到证实。在两组中,都证实了显著的改善。
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引用次数: 0
Different Effects of Low-Level Laser Therapy on the Proliferation of HT29 Cells in Culture and Xenograft Models. 低水平激光治疗对培养和异种移植模型中HT29细胞增殖的不同影响。
IF 1.5 Q2 Medicine Pub Date : 2023-08-30 eCollection Date: 2023-01-01 DOI: 10.34172/jlms.2023.31
Najmeh Malekzadeh Gonabadi, Mehdi Shamsara, Dor Mohammad Kordi Tamandani, Sahar Shojaei, Seyed Mehdi Alavi

Introduction: Different kinds of treatments have been developed to fight cancers. Low-level laser therapy (LLLT), also known as photobiomodulation therapy (PBMT), is a low-power monochromatic and coherent light that has been used successfully for healing injuries and combating malignancies. However, there are concerns about the application of LLLT to cancers due to the increased proliferation of some cancer cells after LLLT. Methods: This study investigated the effects of 650 nm and 870 nm lasers on the proliferation of HT29 colorectal cancer cell lines in vitro and in vivo. Results: The results showed that the laser with a wavelength of 870 nm did not meaningfully alter the proliferation of cultured cells. However, cell proliferation was promoted when the laser was applied within a wavelength of 650 nm. Treatment of HT29-derived tumors in nude mice with the 650 nm laser resulted in the decline of the tumor progression rate compared to controls. This result was inconsistent with the proliferative effects of the laser on the cultured cells. Conclusion: Cell behavior in response to LLLT might be different between cell culture and xenograft models.

引言:已经开发出不同种类的治疗方法来对抗癌症。低水平激光治疗(LLLT),也称为光生物调制治疗(PBMT),是一种低功率单色相干光,已成功用于治疗损伤和对抗恶性肿瘤。然而,由于LLLT后一些癌症细胞的增殖增加,人们担心LLLT在癌症中的应用。方法:研究650nm和870nm激光对HT29结直肠癌癌症细胞株体内外增殖的影响。结果:波长870 nm的激光对培养细胞的增殖没有明显的影响。然而,当在650nm的波长内施加激光时,促进了细胞增殖。与对照组相比,用650nm激光在裸鼠中治疗HT29衍生的肿瘤导致肿瘤进展率下降。这一结果与激光对培养细胞的增殖作用不一致。结论:细胞培养和异种移植物模型对LLLT的反应可能不同。
{"title":"Different Effects of Low-Level Laser Therapy on the Proliferation of HT29 Cells in Culture and Xenograft Models.","authors":"Najmeh Malekzadeh Gonabadi,&nbsp;Mehdi Shamsara,&nbsp;Dor Mohammad Kordi Tamandani,&nbsp;Sahar Shojaei,&nbsp;Seyed Mehdi Alavi","doi":"10.34172/jlms.2023.31","DOIUrl":"https://doi.org/10.34172/jlms.2023.31","url":null,"abstract":"<p><p><b>Introduction:</b> Different kinds of treatments have been developed to fight cancers. Low-level laser therapy (LLLT), also known as photobiomodulation therapy (PBMT), is a low-power monochromatic and coherent light that has been used successfully for healing injuries and combating malignancies. However, there are concerns about the application of LLLT to cancers due to the increased proliferation of some cancer cells after LLLT. <b>Methods:</b> This study investigated the effects of 650 nm and 870 nm lasers on the proliferation of HT29 colorectal cancer cell lines <i>in vitro</i> and <i>in vivo</i>. <b>Results:</b> The results showed that the laser with a wavelength of 870 nm did not meaningfully alter the proliferation of cultured cells. However, cell proliferation was promoted when the laser was applied within a wavelength of 650 nm. Treatment of HT29-derived tumors in nude mice with the 650 nm laser resulted in the decline of the tumor progression rate compared to controls. This result was inconsistent with the proliferative effects of the laser on the cultured cells. <b>Conclusion:</b> Cell behavior in response to LLLT might be different between cell culture and xenograft models.</p>","PeriodicalId":16224,"journal":{"name":"Journal of lasers in medical sciences","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2023-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10517571/pdf/jlms-14-e31.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41163667","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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Journal of lasers in medical sciences
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