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Inhibition of iron-induced cofilin activation and inflammation in microglia by a novel cofilin inhibitor. 一种新型 cofilin 抑制剂可抑制铁诱导的小胶质细胞中 cofilin 的活化和炎症。
IF 4.2 3区 医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-11-18 DOI: 10.1111/jnc.16260
Faheem Shehjar, Antonisamy William James, Reetika Mahajan, Zahoor A Shah

Neuroinflammatory conditions linked to iron dysregulation pose significant challenges in neurodegenerative diseases. Iron-loaded microglia are observed in the brains of patients with various neuroinflammatory conditions, yet how iron overload affects microglial function and contributes to various neuroinflammatory processes is poorly understood. This in vitro study elucidates the relationship between excess iron, cofilin activation, and microglial function, shedding light on potential therapeutic avenues. Iron overload was induced in Human Microglial Clone 3 cells using ferrous sulfate, and the expressions of ferritin heavy chain, ferritin light chain, divalent metal transporter 1, cofilin, p-cofilin, nuclear factor-κB (NF-κB), and various inflammatory cytokines were analyzed using real-time quantitative polymerase chain reaction, immunocytochemistry, Western blotting, and enzyme-linked immunosorbent assay. Results revealed a notable increase in cofilin, NF-κB, and inflammatory cytokine expression levels following excess iron exposure. Moreover, treatment with deferoxamine (DFX), a known iron chelator, and a novel cofilin inhibitor (CI) synthesized in our laboratory demonstrate a mitigating effect on iron-induced cofilin expression. Furthermore, both DFX and CI exhibit promising outcomes in mitigating the inflammatory consequences of excess iron, including the expression of pro-inflammatory cytokines and NF-κB activation. These findings suggest that both DFX and CI can potentially alleviate microglia-induced neuroinflammation by targeting both iron dysregulation and cofilin-mediated pathways. Overall, this study provides valuable insights into iron-induced cofilin activation and microglial activation, offering avenues for potential targeted therapies for neuroinflammatory conditions associated with iron and cofilin dysregulation in neurodegenerative diseases.

与铁失调有关的神经炎症给神经退行性疾病带来了巨大挑战。在各种神经炎症患者的大脑中都能观察到铁负荷过重的小胶质细胞,但人们对铁负荷过重如何影响小胶质细胞功能并导致各种神经炎症过程还知之甚少。这项体外研究阐明了过量铁、cofilin 激活和小胶质细胞功能之间的关系,为潜在的治疗途径提供了启示。使用硫酸亚铁诱导人小胶质细胞克隆 3 细胞铁超载,并使用实时定量聚合酶链反应、免疫细胞化学、Western 印迹和酶联免疫吸附试验分析铁蛋白重链、铁蛋白轻链、二价金属转运体 1、cofilin、p-cofilin、核因子-κB(NF-κB)和各种炎症细胞因子的表达。结果显示,暴露于过量铁后,cofilin、NF-κB 和炎性细胞因子的表达水平显著增加。此外,用一种已知的铁螯合剂去铁胺(DFX)和我们实验室合成的一种新型纤网蛋白抑制剂(CI)进行治疗,可减轻铁诱导的纤网蛋白表达。此外,DFX 和 CI 在减轻过量铁引起的炎症后果(包括促炎症细胞因子的表达和 NF-κB 的激活)方面都表现出良好的效果。这些研究结果表明,DFX 和 CI 有可能通过针对铁失调和 cofilin 介导的途径来减轻小胶质细胞诱导的神经炎症。总之,这项研究为了解铁诱导的cofilin活化和小胶质细胞活化提供了有价值的见解,为治疗神经退行性疾病中与铁和cofilin失调相关的神经炎症提供了潜在的靶向疗法途径。
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引用次数: 0
Neurofilament heavy phosphorylated epitopes as biomarkers in ageing and neurodegenerative disease. 作为老化和神经退行性疾病生物标志物的神经丝蛋白重磷酸化表位。
IF 4.2 3区 医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-11-18 DOI: 10.1111/jnc.16261
Laura F De Paoli, Matthew T K Kirkcaldie, Anna E King, Jessica M Collins

From the day we are born, the nervous system is subject to insult, disease and degeneration. Aberrant phosphorylation states in neurofilaments, the major intermediate filaments of the neuronal cytoskeleton, accompany and mediate many pathological processes in degenerative disease. Neuronal damage, degeneration and death can release these internal components to the extracellular space and eventually the cerebrospinal fluid and blood. Sophisticated assay techniques are increasingly able to detect their presence and phosphorylation states at very low levels, increasing their utility as biomarkers and providing insights and differential diagnosis for the earliest stages of disease. Although a variety of studies focus on single or small clusters of neurofilament phosphorylated epitopes, this review offers a wider perspective of the phosphorylation landscape of the neurofilament heavy subunit, a major intermediate filament component in both ageing and disease.

从我们出生的那一天起,神经系统就开始遭受损伤、疾病和退化。神经元细胞骨架的主要中间丝--神经丝的异常磷酸化状态,伴随并介导着退行性疾病的许多病理过程。神经元损伤、变性和死亡会将这些内部成分释放到细胞外空间,最终进入脑脊液和血液。先进的检测技术越来越能在极低水平上检测到它们的存在和磷酸化状态,从而提高了它们作为生物标记物的效用,并为疾病的早期阶段提供洞察力和鉴别诊断。尽管各种研究都侧重于神经丝蛋白磷酸化表位的单个或小簇,但本综述从更广阔的视角探讨了神经丝蛋白重亚基的磷酸化状况,神经丝蛋白重亚基是老化和疾病中的主要中间丝成分。
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引用次数: 0
Convergent effects of synthetic glucocorticoid dexamethasone and amyloid beta in human olfactory neurosphere-derived cells. 合成糖皮质激素地塞米松和淀粉样蛋白β在人类嗅觉神经球衍生细胞中的趋同效应
IF 4.2 3区 医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-11-18 DOI: 10.1111/jnc.16263
Zane Farnum, Radhika Mani, Aidan Bindoff, Richard Wilson, Adoni Fiotakis, Jessica Stephens, Ellie Cho, Alan Mackay-Sim, Duncan Sinclair

Stressful life events and glucocorticoid (stress) hormones appear to increase the risk of Alzheimer's disease and hasten its progression, but the reasons for this remain unclear. One potential explanation is that when amyloid β (Aβ) pathology is accumulating in the preclinical disease stage, glucocorticoid receptor signalling during stressful events exacerbates cellular dysfunction caused by Aβ. Alternatively, Aβ may disrupt glucocorticoid receptor signalling. To explore these possibilities, we investigated whether the synthetic glucocorticoid dexamethasone and Aβ have overlapping effects on the cellular proteome and whether Aβ influences canonical glucocorticoid receptor function. Human olfactory neurosphere-derived (ONS) cells, collected from the olfactory mucosa of six adult donors, were treated with soluble Aβ40 or Aβ42 followed by dexamethasone. Proteins were quantified by mass spectrometry. After 32 h treatment, Aβ40 and Aβ42 both induced profound changes in innate immunity-related proteins. After 72 h, Aβ42 formed widespread aggregates and induced few proteomic changes, whereas Aβ40 remained soluble and altered expression of mitochondrial and innate immunity-related proteins. ONS cells revealed overlapping impacts of Aβ40 and dexamethasone, with 23 proteins altered by both treatments. For 16 proteins (including eight mitochondrial proteins) dexamethasone counteracted the effects of Aβ40. For example, caspase 4 and methylmalonate-semialdehyde dehydrogenase were increased by Aβ40 and decreased by dexamethasone. Consistent with this finding, Aβ40 increased, but dexamethasone decreased, ONS cell proliferation. For seven proteins, including superoxide dismutase [Mn] mitochondrial, dexamethasone exacerbated the effects of Aβ40. For some proteins, including complement C3, the effects of dexamethasone differed depending on whether Aβ40 was present or absent. Neither Aβ species influenced glucocorticoid receptor nuclear translocation. Overall, this study revealed that glucocorticoid receptor signalling modifies the intracellular effects of Aß40, counteracting some effects and exacerbating others. It suggests that cellular mechanisms through which glucocorticoid receptor signalling influences Alzheimer's disease risk/progression are complex and determined by the balance of beneficial and detrimental glucocorticoid effects.

紧张的生活事件和糖皮质激素(应激)似乎会增加阿尔茨海默病的风险并加速其进展,但其原因仍不清楚。一种可能的解释是,当淀粉样蛋白 β(Aβ)病理学在临床疾病前期积累时,应激事件中的糖皮质激素受体信号会加剧 Aβ 导致的细胞功能障碍。或者,Aβ可能会破坏糖皮质激素受体信号传导。为了探索这些可能性,我们研究了合成糖皮质激素地塞米松和Aβ是否对细胞蛋白质组有重叠作用,以及Aβ是否影响典型糖皮质激素受体的功能。用可溶性 Aβ40 或 Aβ42 处理人嗅神经球(ONS)细胞(采集自六名成年供体的嗅粘膜),然后用地塞米松处理。蛋白质通过质谱法进行定量。处理 32 小时后,Aβ40 和 Aβ42 均诱导先天免疫相关蛋白发生深刻变化。72 小时后,Aβ42 形成广泛的聚集体,诱导的蛋白质组变化很小,而 Aβ40 仍保持可溶性,并改变了线粒体和先天免疫相关蛋白质的表达。ONS细胞显示,Aβ40和地塞米松的影响是重叠的,两种处理方法都改变了23种蛋白质。地塞米松抵消了Aβ40对16种蛋白质(包括8种线粒体蛋白质)的影响。例如,Aβ40 使 Caspase 4 和甲基丙二酸半醛脱氢酶增加,而地塞米松使其减少。与这一发现相一致的是,Aβ40 增加了 ONS 细胞的增殖,而地塞米松则减少了增殖。对于包括线粒体超氧化物歧化酶[Mn]在内的七种蛋白质,地塞米松加剧了 Aβ40 的作用。对于补体 C3 等一些蛋白质,地塞米松的作用因 Aβ40 的存在与否而不同。两种 Aβ 均不影响糖皮质激素受体的核转位。总之,这项研究揭示了糖皮质激素受体信号改变了 Aß40 的细胞内效应,抵消了一些效应,加剧了另一些效应。研究表明,糖皮质激素受体信号影响阿尔茨海默病风险/进展的细胞机制是复杂的,并由糖皮质激素的有益和有害效应的平衡决定。
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引用次数: 0
A special focus on polyadenylation and alternative polyadenylation in neurodegenerative diseases: A systematic review. 特别关注神经退行性疾病中的多聚腺苷酸化和替代多聚腺苷酸化:系统综述。
IF 4.2 3区 医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-11-18 DOI: 10.1111/jnc.16255
Tarlan Yeganeh Markid, Azam Pourahmadiyan, Soroosh Hamzeh, Mirmohsen Sharifi-Bonab, Mohamad Reza Asadi, Abbas Jalaiei, Maryam Rezazadeh, Soudeh Ghafouri-Fard

Neurodegenerative diseases (NDDs) are one of the prevailing conditions characterized by progressive neuronal loss. Polyadenylation (PA) and alternative polyadenylation (APA) are the two main post-transcriptional events that regulate neuronal gene expression and protein production. This systematic review analyzed the available literature on the role of PA and APA in NDDs, with an emphasis on their contributions to disease development. A comprehensive literature search was performed using the PubMed, Scopus, Cochrane, Google Scholar, Embase, Web of Science, and ProQuest databases. The search strategy was developed based on the framework introduced by Arksey and O'Malley and supplemented by the inclusion and exclusion criteria. The study selection was performed by two independent reviewers. Extraction and data organization were performed in accordance with the predefined variables. Subsequently, quantitative and qualitative analyses were performed. Forty-seven studies were included, related to a variety of NDDs, namely Alzheimer's disease, Parkinson's disease, Huntington's disease, and amyotrophic lateral sclerosis. Disease induction was performed using different models, including human tissues, animal models, and cultured cells. Most investigations were related to PA, although some were related to APA or both. Amyloid precursor protein (APP), Tau, SNCA, and STMN2 were the major genes identified; most of the altered PA patterns were related to mRNA stability and translation efficiency. This review particularly underscores the key roles of PA and APA in the pathogenesis of NDDs through their mechanisms that contribute to gene expression dysregulation, protein aggregation, and neuronal dysfunction. Insights into these mechanisms may lead to new therapeutic strategies focused on the modulation of PA and APA activities. Further research is required to investigate the translational potential of targeting these pathways for NDD treatment.

神经退行性疾病(NDDs)是以神经元逐渐丧失为特征的常见疾病之一。多腺苷酸化(PA)和替代多腺苷酸化(APA)是调节神经元基因表达和蛋白质生成的两个主要转录后事件。本系统性综述分析了有关 PA 和 APA 在 NDDs 中作用的现有文献,重点是它们对疾病发展的贡献。我们使用 PubMed、Scopus、Cochrane、Google Scholar、Embase、Web of Science 和 ProQuest 数据库进行了全面的文献检索。检索策略是根据 Arksey 和 O'Malley 提出的框架制定的,并辅以纳入和排除标准。研究选题由两名独立审稿人完成。根据预先确定的变量进行数据提取和整理。随后,进行了定量和定性分析。共纳入 47 项研究,涉及多种 NDD,即阿尔茨海默病、帕金森病、亨廷顿病和肌萎缩侧索硬化症。疾病诱导使用了不同的模型,包括人体组织、动物模型和培养细胞。大多数研究与 PA 有关,但也有一些研究与 APA 或两者都有关。淀粉样前体蛋白(APP)、Tau、SNCA 和 STMN2 是已发现的主要基因;大多数 PA 模式的改变与 mRNA 的稳定性和翻译效率有关。这篇综述特别强调了 PA 和 APA 在 NDD 发病机制中的关键作用,因为它们的机制导致了基因表达失调、蛋白聚集和神经元功能障碍。对这些机制的深入了解可能会带来以调节 PA 和 APA 活性为重点的新治疗策略。要研究针对这些途径治疗 NDD 的转化潜力,还需要进一步的研究。
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引用次数: 0
Modulating the cholinergic system-Novel targets for deep brain stimulation in Parkinson's disease. 调节胆碱能系统--帕金森病深部脑刺激的新目标。
IF 4.2 3区 医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-11-18 DOI: 10.1111/jnc.16264
V Witzig, R Pjontek, S K H Tan, J B Schulz, F Holtbernd

Parkinson's disease (PD) is the second-fastest growing neurodegenerative disease in the world. The major clinical symptoms rigor, tremor, and bradykinesia derive from the degeneration of the nigrostriatal pathway. However, PD is a multi-system disease, and neurodegeneration extends beyond the degradation of the dopaminergic pathway. Symptoms such as postural instability, freezing of gait, falls, and cognitive decline are predominantly caused by alterations of transmitter systems outside the classical dopaminergic axis. While levodopa and deep brain stimulation (DBS) of the subthalamic nucleus or globus pallidus internus effectively address PD primary motor symptoms, they often fall short in mitigating axial symptoms and cognitive impairment. Along these lines, the cholinergic system is increasingly recognized to play a crucial role in governing locomotion, postural stability, and cognitive function. Thus, there is a growing interest in bolstering the cholinergic tone by DBS of cholinergic targets such as the pedunculopontine nucleus (PPN) and nucleus basalis of Meynert (NBM), aiming to alleviate these debilitating symptoms resistant to traditional treatment strategies targeting the dopaminergic network. This review offers a comprehensive overview of the role of cholinergic dysfunction in PD. We discuss the impact of PPN and NBM DBS on the management of symptoms not readily accessible to established DBS targets and pharmacotherapy in PD and seek to provide guidance on patient selection, surgical approach, and stimulation paradigms.

帕金森病(Parkinson's disease,PD)是世界上发病率第二高的神经退行性疾病。其主要临床症状为僵直、震颤和运动迟缓,源于黑质纹状体通路的退化。然而,帕金森病是一种多系统疾病,神经退行性变不仅仅局限于多巴胺能通路的退化。姿势不稳、步态冻结、跌倒和认知能力下降等症状主要是由经典多巴胺能轴以外的递质系统改变引起的。虽然左旋多巴和对眼下核或苍白球内核的脑深部刺激(DBS)能有效解决帕金森病的主要运动症状,但它们往往无法减轻轴向症状和认知障碍。因此,人们越来越认识到胆碱能系统在支配运动、姿势稳定性和认知功能方面发挥着至关重要的作用。因此,越来越多的人开始关注通过对胆碱能靶点(如足底核(PPN)和梅内特基底核(NBM))进行 DBS 治疗来增强胆碱能张力,从而缓解这些对针对多巴胺能网络的传统治疗策略产生抗药性的衰弱症状。本综述全面概述了胆碱能功能障碍在帕金森病中的作用。我们讨论了 PPN 和 NBM DBS 对治疗帕金森病中既有 DBS 靶点和药物疗法难以奏效的症状的影响,并试图为患者选择、手术方法和刺激范式提供指导。
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引用次数: 0
Alterations of endocannabinoid signaling and microglia reactivity in the retinas of AD-like mice precede the onset of hippocampal β-amyloid plaques. AD样小鼠视网膜中内源性大麻素信号传导和小胶质细胞反应性的改变先于海马β淀粉样蛋白斑块的出现。
IF 4.2 3区 医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-11-18 DOI: 10.1111/jnc.16256
Annamaria Tisi, Lucia Scipioni, Giulia Carozza, Lucia Di Re, Giacomo Cimino, Camilla Di Meo, Sakthimala Palaniappan, Francesco Della Valle, Federico Fanti, Giacomo Giacovazzo, Dario Compagnone, Rita Maccarone, Sergio Oddi, Mauro Maccarrone

Extra-cerebral manifestations of Alzheimer's disease (AD) develop in the retina, which is, therefore, considered a "window to the brain". Recent studies demonstrated the dysregulation of the endocannabinoid (eCB) system (ECS) in AD brain. Here, we explored the possible alterations of ECS and the onset of gliosis in the retina of AD-like mice. Tg2576 (TG) mice overexpressing the amyloid precursor protein (APP) were used at the age of 12 months, when hippocampal β-amyloid plaques had not been developed yet. Analysis of retinal gliosis showed a significant increase in the number of IBA1 (+) microglia cells in TG versus wild type (WT). Gliosis was not associated with retinal β-amyloid plaques, evident retinal degenerative signatures, or excitotoxicity; instead, oxidative stress burden was observed as increased acrolein levels. Analysis of the ECS (receptors/metabolic enzymes) through western blotting (WB) revealed the up-regulation of cannabinoid receptor 2 (CB2) and monoacylglycerol lipase (MAGL), the enzyme responsible for the degradation of 2-arachidonoylglycerol (2-AG), in TG retinas. Fluorescence intensity analysis of anti-CB2 and anti-MAGL immuno-stained cryosections was consistent with WB, showing their up-regulation throughout the retinal layers. No statistically significant differences were found for the other enzymes/receptors of the ECS under study. However, linear regression analysis for individual animals showed a significant correlation between CB2 and fatty acid amide hydrolase (FAAH), diacylglycerol lipase α/β (DAGLα/β), and APP; instead, a significant negative correlation was found between MAGL and APP. Finally, ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) demonstrated a significant reduction of 2-AG in TG retinas (~0.34 ng/mg) compared to WT (~1.70 ng/mg), while a trend toward increase was found for the other eCB anandamide (AEA). Overall, our data indicate that gliosis and ECS dysregulation-in particular of CB2, MAGL and 2-AG-occur in the retina of AD-like mice before retinal degeneration and development of hippocampal β-amyloid plaques.

阿尔茨海默病(AD)的脑外表现发生在视网膜,因此视网膜被认为是 "通往大脑的窗口"。最近的研究表明,AD 大脑中的内源性大麻素(eCB)系统(ECS)失调。在此,我们探讨了ECS的可能改变以及AD样小鼠视网膜胶质病变的发生。我们使用了过表达淀粉样前体蛋白(APP)的Tg2576(TG)小鼠,这些小鼠在12个月大时海马β淀粉样斑块尚未形成。视网膜胶质增生分析表明,与野生型(WT)相比,TG 的 IBA1(+)小胶质细胞数量显著增加。神经胶质增生与视网膜β-淀粉样蛋白斑块、明显的视网膜变性特征或兴奋性毒性无关;相反,氧化应激负担表现为丙烯醛水平的增加。通过 Western 印迹(WB)分析 ECS(受体/代谢酶)发现,在 TG 视网膜中,大麻素受体 2(CB2)和单酰甘油脂肪酶(MAGL)上调,后者是负责降解 2-arachidonoylglycerol (2-AG) 的酶。抗 CB2 和抗 MAGL 免疫染色冰冻切片的荧光强度分析与 WB 一致,显示它们在整个视网膜层中上调。所研究的 ECS 的其他酶/受体在统计学上没有发现明显差异。然而,对单个动物的线性回归分析表明,CB2 与脂肪酸酰胺水解酶(FAAH)、二酰甘油脂肪酶 α/β(DAGLα/β)和 APP 之间存在显著相关性;相反,MAGL 与 APP 之间存在显著负相关。最后,超高效液相色谱-串联质谱法(UPLC-MS/MS)显示,与 WT(约 1.70 ng/mg)相比,TG 视网膜中的 2-AG 显著减少(约 0.34 ng/mg),而另一种 eCB-anandamide(AEA)则呈上升趋势。总之,我们的数据表明,在视网膜退化和海马β-淀粉样蛋白斑块形成之前,类似AD的小鼠视网膜中会出现神经胶质增生和ECS失调,尤其是CB2、MAGL和2-AG。
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引用次数: 0
Elevated serum sodium is linked to increased amyloid-dependent tau pathology, neurodegeneration, and cognitive impairment in Alzheimer's disease. 血清钠的升高与阿尔茨海默病中淀粉样蛋白依赖性 tau 病变、神经变性和认知障碍的增加有关。
IF 4.2 3区 医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-11-06 DOI: 10.1111/jnc.16257
Yu-Han Chen, Zhi-Bo Wang, Xi-Peng Liu, Zhi-Qi Mao

Vascular dysfunction is implicated in the pathophysiology of Alzheimer's disease (AD). While sodium is essential for maintaining vascular function, its role in AD pathology remains unclear. We included 353 participants from the Alzheimer's Disease Neuroimaging Initiative (ADNI), assessing serum sodium levels, cerebrospinal fluid (CSF) and positron emission tomography (PET) biomarkers, magnetic resonance imaging (MRI), and cognitive function. An independent sample (N = 471) with available CSF sodium-related proteins and AD biomarkers was also included. Associations between serum sodium levels and AD pathology, neurodegeneration, and cognition were evaluated using linear regression models. Spearman's correlation analyses assessed the relationships between CSF sodium-related proteins and AD biomarkers. Higher serum sodium levels were associated with increased AD pathology, reduced hippocampal volume, and greater cognitive decline (all p < 0.05). The relationship between serum sodium and amyloid PET was evident in several AD-susceptible brain regions, including the neocortex and limbic system. Individuals with high serum sodium exhibited higher tau pathology, lower hippocampal volume, and more severe cognitive decline per unit increase in amyloid PET compared to those with low serum sodium (all p < 0.05). Among the 14 CSF sodium-related proteins, which were inter-correlated, six were significantly correlated with CSF AD pathology and amyloid PET, while two were correlated with hippocampal volume and cognitive function, with sodium channel subunit beta-2 (SCN2B) and sodium channel subunit beta-3 (SCN3B) showing the strongest correlations. These findings underscore the crucial role of serum sodium in AD progression, highlighting a potential network of sodium dysregulation involved in AD pathology. Targeting sodium may offer a novel therapeutic approach to slowing AD progression, particularly by impeding the progression of amyloid-related downstream events.

血管功能障碍与阿尔茨海默病(AD)的病理生理学有关。虽然钠对维持血管功能至关重要,但它在阿尔茨海默病病理中的作用仍不清楚。我们纳入了 353 名阿尔茨海默病神经影像学倡议(ADNI)的参与者,评估他们的血清钠水平、脑脊液(CSF)和正电子发射断层扫描(PET)生物标志物、磁共振成像(MRI)和认知功能。此外还包括一个独立样本(N = 471),该样本具有可用的脑脊液钠相关蛋白和注意力缺失症生物标志物。使用线性回归模型评估了血清钠水平与 AD 病理学、神经变性和认知能力之间的关系。斯皮尔曼相关性分析评估了 CSF 钠相关蛋白和 AD 生物标志物之间的关系。血清钠水平越高,AD 病变越严重,海马体积越小,认知能力下降越严重(所有 p
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引用次数: 0
The activation of inflammatory responses in the brain is potentiated by over-expressing acetylcholinesterase in myeloid lineage of transgenic mice. 在转基因小鼠的髓系中过度表达乙酰胆碱酯酶,可增强大脑炎症反应的激活作用。
IF 4.2 3区 医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-11-05 DOI: 10.1111/jnc.16251
Yingjie Xia, Xiaoyang Wang, Maggie Suisui Guo, Jiahui Wu, Jin Gao, Tina T X Dong, Karl W K Tsim

Acetylcholinesterase (AChE) has functions in neuroinflammation, beyond its classical role in neurotransmission. Understanding the role of AChE in neuroinflammation is of great significance, as it highlights the potential therapeutic targets for the treatment of neurodegenerative diseases. In an in vitro study, the expression of AChE was up-regulated in lipopolysaccharide (LPS)-induced microglia/macrophage and contrarily potentiated the inflammatory responses via disturbing the cholinergic anti-inflammatory pathway (CAP). However, the regulation of AChE in neuroinflammation has not been revealed in vivo yet. Here, we aim to uncover the inflammatory roles of microglial AChE in LPS-induced neuroinflammation by using the conditional AChE over-expression mouse model. AChE was specifically over-expressed in the myeloid cell linkage of mouse by applying CRISPR/cas9 combined with Cre-LoxP system. LPS was intraperitoneally injected into the mice to induce inflammation. The results showed that the inflammation, induced by LPS, was aggravated in the brain of transgenic mice having over-expression of AChE in microglia. The expressions of pro-inflammatory cytokines were robustly up-regulated in the brains of LPS-treated transgenic mice, as compared to the LPS-treated wildtypes. In parallel, the activations of microglia and astrocytes in hippocampus were enhanced significantly in AChE transgenic mice. Transcriptomics analysis further confirmed the severer inflammation in the transgenic mice than the wildtype after LPS administration. These findings shed light on the regulation of microglial AChE in neuroinflammation in vivo for the first time, presenting another angle to understand the role of AChE in neurodegenerative diseases.

乙酰胆碱酯酶(AChE)除了在神经传导中发挥传统作用外,还在神经炎症中发挥作用。了解乙酰胆碱酯酶在神经炎症中的作用意义重大,因为它凸显了治疗神经退行性疾病的潜在治疗靶点。在一项体外研究中,AChE 在脂多糖(LPS)诱导的小胶质细胞/巨噬细胞中表达上调,并通过干扰胆碱能抗炎途径(CAP)增强炎症反应。然而,AChE 在神经炎症中的调控尚未在体内得到揭示。在这里,我们利用条件性 AChE 过度表达小鼠模型,旨在揭示小胶质细胞 AChE 在 LPS 诱导的神经炎症中的炎症作用。通过CRISPR/cas9结合Cre-LoxP系统,AChE被特异性地过度表达在小鼠的髓系细胞连接中。小鼠腹腔注射 LPS 诱导炎症。结果表明,在小胶质细胞中 AChE 过度表达的转基因小鼠脑内,LPS 诱导的炎症加剧。与经 LPS 处理的野生型小鼠相比,经 LPS 处理的转基因小鼠脑内促炎细胞因子的表达明显上调。与此同时,AChE 转基因小鼠海马中的小胶质细胞和星形胶质细胞的活化也显著增强。转录组学分析进一步证实,在给予 LPS 后,转基因小鼠的炎症比野生型严重。这些发现首次揭示了小胶质细胞 AChE 在体内神经炎症中的调控作用,为了解 AChE 在神经退行性疾病中的作用提供了另一个角度。
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引用次数: 0
Causal association between insulin sensitivity index and Alzheimer's disease. 胰岛素敏感性指数与阿尔茨海默病之间的因果关系。
IF 4.2 3区 医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-10-31 DOI: 10.1111/jnc.16254
Fang Xu, Shiyang Wu, Shan Gao, Xuan Li, Chen Huang, Yan Chen, Ping Zhu, Guiyou Liu

Evidence from observational and Mendelian randomization (MR) studies suggested that insulin resistance (IR) was associated with Alzheimer's disease (AD). However, the causal effects of different indicators of IR on AD remain inconsistent. Here, we aim to assess the causal association between the insulin sensitivity index (ISI), a measure of post-prandial IR, and the risk of AD. We first conducted primary and secondary univariable MR analyses. We selected 8 independent genome-wide significant (p < 5E-08, primary analyses) and 61 suggestive (p < 1E-05, secondary analyses) ISI genetic variants from large-scale genome-wide association studies (GWAS; N = 53 657), respectively, and extracted their corresponding GWAS summary statistics from AD GWAS, including IGAP2019 (N = 63 926) and FinnGen_G6_AD_WIDE (N = 412 181). We selected five univariable MR methods and used heterogeneity, horizontal pleiotropy test, and leave-one-out sensitivity analysis to confirm the stability of MR estimates. Finally, we conducted a meta-analysis to combine MR estimates from two non-overlapping AD GWAS datasets. We further performed multivariable MR (MVMR) to assess the potential mediating role of type 2 diabetes (T2D) on the association between ISI and AD using two MVMR methods. In univariable MR, utilizing 8 genetic variants in primary analyses, we found a significant causal association of genetically increased ISI with decreased risk of AD (OR = 0.79, 95% CI: 0.68-0.92, p = 0.003). Utilizing 61 genetic variants in secondary analyses, we found consistent findings of a causal effect of genetically increased ISI on the decreased risk of AD (OR = 0.89, 95% CI: 0.82-0.96, p = 0.003). Heterogeneity, horizontal pleiotropy test, and leave-one-out sensitivity analysis ensured the reliability of the MR estimates. In MVMR, we found no causal relationship between ISI and AD after adjusting for T2D (p > 0.05). We provide genetic evidence that increased ISI is significantly and causally associated with reduced risk of AD, which is mediated by T2D. These findings may inform prevention strategies directed toward IR-associated T2D and AD.

观察性研究和孟德尔随机化(MR)研究的证据表明,胰岛素抵抗(IR)与阿尔茨海默病(AD)有关。然而,不同的胰岛素抵抗指标对阿尔茨海默病的因果效应仍不一致。在此,我们旨在评估餐后胰岛素敏感性指数(ISI)与阿尔茨海默病风险之间的因果关系。我们首先进行了主要和次要的单变量 MR 分析。我们选择了 8 个独立的全基因组显著性(P 0.05)。我们提供的遗传学证据表明,ISI的增加与AD风险的降低有显著的因果关系,而AD风险的降低是由T2D介导的。这些发现可为针对与 IR 相关的 T2D 和 AD 的预防策略提供依据。
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引用次数: 0
Circulating medium- and long-chain acylcarnitines are associated with plasma P-tau181 in cognitively normal older adults. 认知能力正常的老年人体内循环的中链和长链酰基肉碱与血浆 P-tau181 相关。
IF 4.2 3区 医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-10-30 DOI: 10.1111/jnc.16244
Tahmida Sharmin, Pratishtha Chatterjee, James D Doecke, Nicholas J Ashton, Kevin Huynh, Steve Pedrini, Hamid R Sohrabi, Benjamin Heng, Shaun Eslick, Henrik Zetterberg, Kaj Blennow, Manohar Garg, Ralph N Martins

Alzheimer's disease (AD) pathogenesis involves dysregulation in diverse biochemical processes. Nevertheless, plasma tau phosphorylated at threonine 181 (P-tau181), a recognised AD biomarker, has been described to reflect early-stage cortical amyloid-β (Aβ) deposition in cognitively normal (CN) adults. Therefore, identifying changes in plasma metabolites associated with plasma P-tau181 at the pre-clinical stage may provide insights into underlying biochemical mechanisms to better understand initial AD pathogenesis. In the current study, plasma P-tau181, quantified via single molecule array (Simoa) technology, and plasma metabolites, quantified via targeted-mass spectrometry, were investigated for associations in CN older adults and upon stratification by positron emission tomography (PET)-Aβ load. In addition, the P-tau181-linked metabolites were evaluated for cognitive performance and neuroimaging markers of AD and the potential to distinguish between CN Aβ- and CN Aβ+ individuals. Significant positive associations of medium- and long-chain acylcarnitines (ACs) were observed with P-tau181 in the entire cohort, CN Aβ- and CN Aβ+, suggesting a link between initial Aβ pathology and fatty acid oxidation-mediated energy metabolism pathways. However, in CN Aβ-, additional linear associations of P-tau181 were observed with muscle metabolism and nitric oxide homeostasis-associated metabolites. Upon investigating the P-tau181-linked metabolites for cognitive performance, significant inverse correlations of the verbal and visual episodic memory and the global composite score were noted in CN Aβ+ with medium- and long-chain ACs, suggesting prognostic value of ACs accompanying weaker cognitive performance. While investigating neuroimaging markers, ACs had positive associations with PET-Aβ load and inverse associations with hippocampal volume in CN Aβ+, indicating connections of ACs with initial AD pathogenesis. Furthermore, based on receiver operating characteristics analysis, the associated ACs potentially classified PET-Aβ status in older adults. Therefore, plasma P-tau181-linked circulating ACs may serve as potential prognostic markers for initial AD pathogenesis in CN older adults. However, further cross-sectional and longitudinal research in highly characterised AD cohorts is needed to validate current findings.

阿尔茨海默病(AD)的发病机制涉及多种生化过程的失调。然而,血浆中苏氨酸181磷酸化的tau(P-tau181)是公认的阿尔茨海默病生物标志物,据描述可反映认知能力正常(CN)成年人皮质淀粉样蛋白-β(Aβ)沉积的早期阶段。因此,在临床前阶段确定与血浆P-tau181相关的血浆代谢物的变化可能有助于深入了解潜在的生化机制,从而更好地理解AD的初期发病机制。在目前的研究中,通过单分子阵列(Simoa)技术量化的血浆P-tau181和通过靶向质谱技术量化的血浆代谢物被用于研究中老年人的相关性,并根据正电子发射断层扫描(PET)-Aβ负荷进行分层。此外,还评估了与 P-tau181 相关联的代谢物与认知能力和注意力缺失症神经影像学标志物的关系,以及区分 CN Aβ- 和 CN Aβ+ 人群的潜力。在整个队列、CN Aβ-和CN Aβ+人群中,观察到中链和长链酰基肉碱(AC)与P-tau181呈显著正相关,这表明最初的Aβ病理学与脂肪酸氧化介导的能量代谢途径之间存在联系。然而,在 CN Aβ- 中,还观察到 P-tau181 与肌肉代谢和一氧化氮稳态相关代谢物的线性关系。在研究 P-tau181 相关代谢物与认知能力的关系时,发现在中链和长链 AC 的 CN Aβ+ 中,言语和视觉外显记忆与总体综合评分呈显著的反向相关性,这表明伴随认知能力减弱的 AC 具有预后价值。在研究神经影像标记物时,ACs 与 PET-Aβ 负荷呈正相关,与 CN Aβ+ 的海马体积呈反相关,表明 ACs 与最初的 AD 发病机制有关。此外,根据接收器操作特性分析,相关的AC可对老年人的PET-Aβ状态进行分类。因此,血浆中与 P-tau181 链接的循环 ACs 可作为 CN 老年人初期 AD 发病的潜在预后标志物。不过,还需要在高度特征化的AD队列中开展进一步的横断面和纵向研究,以验证目前的发现。
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Journal of Neurochemistry
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