Ghasem Valizadeh, S. Ebrahimi, Morteza Gholami, Mohsen Mazaheritehrani, H. Rezadoost, A. Sonboli
Abbreviations: DPPH, 2,2-Diphenyl-1-picrylhydrazyl; BHT, Butylated Hydroxytoluene; CC, Column Chromatography; RSA, Radical Scavenging Activity; HMBC, Heteronuclear Multiple Bond Correlation; HSQC, Heteronuclear Single Quantum Coherence Background: Medicinal plants play a significant role in preventing and treating diseases due to their traditional uses. Objective: The aim of the study was to isolate, purify and identify phytochemicals from the n- hexane extract of Teucrium hircanicum L. roots and evaluate the antioxidant activity of the extract and its purified compounds. Methods: The n- hexane extract (2.27 g) was extracted from the roots of this plant. The extract was fractionated by column chromatography with normal phase by eluting with n- hexane-EtOAc and following with EtOAc-Methanol. Fractions with similar phytochemical fingerprints combined to produce 23 main fractions. Final purification was carried out by preparative reversed-phase HPLC-UV. The structures of isolated were secured by different spectroscopic methods such as 1D, 2D NMR, and mass spectroscopy methods and comparing of these data with literature reported values. The antioxidant activity of the n- hexane extract of T. hircanicum roots and its purified diterpenoids was evaluated in DPPH assay and radical scavenging activity was calculated. Results: Extraction and isolation methods were used to purify three rearranged abietane-type diterpenoids villosin A ( 1 ), teuvincenone B ( 2 ) and 5, 8, 11, 13, 15-abietapentaen-7-one ( 3) , a phenethyl ester namely 4-hydroxyphenethyl pentacosanoate ( 4 ) and one sterol namely 22-dehydroclerosterol ( 5 ). Conclusion: The n- hexane extract from roots of T. hircanicum and three rearranged abietane type diterpenoids were showed good antioxidant activities ranged from 3.5-4.3 μg/ml compared with the reference value (BHT = 16.5 μg/ml).
{"title":"Rearranged abietane diterpenoids from roots of Teucrium hircanicum L.","authors":"Ghasem Valizadeh, S. Ebrahimi, Morteza Gholami, Mohsen Mazaheritehrani, H. Rezadoost, A. Sonboli","doi":"10.52547/jmp.20.79.24","DOIUrl":"https://doi.org/10.52547/jmp.20.79.24","url":null,"abstract":"Abbreviations: DPPH, 2,2-Diphenyl-1-picrylhydrazyl; BHT, Butylated Hydroxytoluene; CC, Column Chromatography; RSA, Radical Scavenging Activity; HMBC, Heteronuclear Multiple Bond Correlation; HSQC, Heteronuclear Single Quantum Coherence Background: Medicinal plants play a significant role in preventing and treating diseases due to their traditional uses. Objective: The aim of the study was to isolate, purify and identify phytochemicals from the n- hexane extract of Teucrium hircanicum L. roots and evaluate the antioxidant activity of the extract and its purified compounds. Methods: The n- hexane extract (2.27 g) was extracted from the roots of this plant. The extract was fractionated by column chromatography with normal phase by eluting with n- hexane-EtOAc and following with EtOAc-Methanol. Fractions with similar phytochemical fingerprints combined to produce 23 main fractions. Final purification was carried out by preparative reversed-phase HPLC-UV. The structures of isolated were secured by different spectroscopic methods such as 1D, 2D NMR, and mass spectroscopy methods and comparing of these data with literature reported values. The antioxidant activity of the n- hexane extract of T. hircanicum roots and its purified diterpenoids was evaluated in DPPH assay and radical scavenging activity was calculated. Results: Extraction and isolation methods were used to purify three rearranged abietane-type diterpenoids villosin A ( 1 ), teuvincenone B ( 2 ) and 5, 8, 11, 13, 15-abietapentaen-7-one ( 3) , a phenethyl ester namely 4-hydroxyphenethyl pentacosanoate ( 4 ) and one sterol namely 22-dehydroclerosterol ( 5 ). Conclusion: The n- hexane extract from roots of T. hircanicum and three rearranged abietane type diterpenoids were showed good antioxidant activities ranged from 3.5-4.3 μg/ml compared with the reference value (BHT = 16.5 μg/ml).","PeriodicalId":16582,"journal":{"name":"Journal of Medicinal Plants","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46545090","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
is a medicinal plant from Zygophyllaceae family. This of 27 genera and 285 species. Objective: The present study was performed to evaluate the chemical composition of the essential oil of Z. eichwaldii and evaluation of biological activities of its extracts . Methods: Essential oils extraction of Z. eichwaldii aerial parts was performed by three different procedures and its chemical composition were evaluated by GC and GC-MS analyses. Evaluation of the biological activities was carried out by spectrophotometric methods. Results: GC-MS analysis showed menthol, thymol and palmitic acid as major components of the plant essential oils. In biological activity evaluation, while methanol extract showed moderate to week potency in antioxidant assessments, the ethyl acetate fraction was strong in these tests (IC 50 = 178.63 µg/ml in DPPH assay and 70.52 % inhibition in β-carotene/linoleic acid tests). This fraction also showed significant phenol, flavonoid and tannin contents (99.83, 118.29 and 188.05 μg/mg, respectively). Also, plant extracts exhibited considerable antimicrobial activities against most selected bacteria. Conclusion: Significant amount of phenol, flavonoid and tannin compounds in the ethyl acetate fraction and high antimicrobial activity against most bacterial strains, candidates this plant as a good case for further studies in this respect.
{"title":"Essential oil analysis and biological activities of the aerial parts of Zygophyllum eichwaldii C. A. Mey., a native plant from Iran","authors":"A. Mazoochi, S. A. Pourmousavi, A. Bamoniri","doi":"10.52547/jmp.20.79.85","DOIUrl":"https://doi.org/10.52547/jmp.20.79.85","url":null,"abstract":"is a medicinal plant from Zygophyllaceae family. This of 27 genera and 285 species. Objective: The present study was performed to evaluate the chemical composition of the essential oil of Z. eichwaldii and evaluation of biological activities of its extracts . Methods: Essential oils extraction of Z. eichwaldii aerial parts was performed by three different procedures and its chemical composition were evaluated by GC and GC-MS analyses. Evaluation of the biological activities was carried out by spectrophotometric methods. Results: GC-MS analysis showed menthol, thymol and palmitic acid as major components of the plant essential oils. In biological activity evaluation, while methanol extract showed moderate to week potency in antioxidant assessments, the ethyl acetate fraction was strong in these tests (IC 50 = 178.63 µg/ml in DPPH assay and 70.52 % inhibition in β-carotene/linoleic acid tests). This fraction also showed significant phenol, flavonoid and tannin contents (99.83, 118.29 and 188.05 μg/mg, respectively). Also, plant extracts exhibited considerable antimicrobial activities against most selected bacteria. Conclusion: Significant amount of phenol, flavonoid and tannin compounds in the ethyl acetate fraction and high antimicrobial activity against most bacterial strains, candidates this plant as a good case for further studies in this respect.","PeriodicalId":16582,"journal":{"name":"Journal of Medicinal Plants","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48720451","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Khanavi, S. Ghadami, Gh Sadaghiani-Tabrizi, M. Delnavazi
Kelussia odoratissima Mozaff. from Apiaceae family is a perennial herbaceous plant endemic to the west of Iran. The aromatic aerial parts of this species are traditionally used by indigenous people to flavor some local foods, as well as for various therapeutic purposes. Objective: The present study was designed to analyze phenolic compounds and essential oil constituents of K . odoratissima fruits. Methods: The n -butanol fraction obtained from hydroalcoholic extract of K. odoratissima fruits was investigated by chromatography on normal phase and Sephadex LH-20 columns. Chemical structures of the isolated compounds were clarified by ¹H-NMR and ¹³C-NMR spectral analyses. Essential oil constituents of the fruits were also analyzed using GC-MS. Results: Phytochemical investigation of the K. odoratissima fruits resulted in the isolation of five flavonol glycosides; isorhamnetin 3-O-glucoside ( 1 ), quercetin 3-O-glucoside (isoquercetin) ( 2 ), isorhamnetin 3-O-rutinoside (narcissin) ( 3 ), isorhamnetin 3-O-glucuronide ( 4 ) and quercetin 3-O-glucuronide (mequilianin) ( 5 ). GC-MS analysis of the fruits essential oil led to the identification of the thirty six compounds, of which ( Z )-ligustilide (15.93 %), δ -cadinene (12.26 %) and germacrene D (12.18 %) were the main compounds. Conclusion: The results of this study introduce K. odoratissima fruits as a source of flavonoid glycosides and phthalate derivatives. The presence of these compounds with known biological properties and health beneficial effects provides more medicinal potentials for the fruits of K. odoratissima and suggest it an appropriate option for further studies.
莫札夫。来自蜂科是伊朗西部特有的多年生草本植物。这种植物的芳香部分传统上被土著居民用来给一些当地食物调味，以及用于各种治疗目的。目的:对白藜芦醇的酚类化合物和挥发油成分进行分析。odoratissima水果。方法:采用正相色谱法和Sephadex LH-20柱色谱法对香果水醇提取物的正丁醇组分进行分析。通过¹H-NMR和¹³C-NMR谱分析确定了化合物的化学结构。同时采用气相色谱-质谱法分析了果实的挥发油成分。结果:通过植物化学分析，分离得到5种黄酮醇苷类化合物;异鼠李素3- o -葡萄糖苷(1)、槲皮素3- o -葡萄糖苷(异槲皮素)(2)、异鼠李素3- o -芦丁苷(水仙素)(3)、异鼠李素3- o -葡萄糖苷(4)和槲皮素3- o -葡萄糖苷(甲酰基氨基)(5)。GC-MS分析鉴定出36种化合物，其中(Z)- liguslide(15.93%)、δ -cadinene(12.26%)和germacene D(12.18%)为主要化合物。结论:本研究结果表明，臭臭果可作为类黄酮苷和邻苯二甲酸酯衍生物的来源。这些具有已知生物学特性和有益健康作用的化合物的存在为臭臭果提供了更多的药用潜力，并为进一步研究提供了合适的选择。
{"title":"Phytochemical constituents of the fruits of Kelussia odoratissima Mozaff., an aromatic plant endemic to Iran","authors":"M. Khanavi, S. Ghadami, Gh Sadaghiani-Tabrizi, M. Delnavazi","doi":"10.52547/jmp.20.79.1","DOIUrl":"https://doi.org/10.52547/jmp.20.79.1","url":null,"abstract":"Kelussia odoratissima Mozaff. from Apiaceae family is a perennial herbaceous plant endemic to the west of Iran. The aromatic aerial parts of this species are traditionally used by indigenous people to flavor some local foods, as well as for various therapeutic purposes. Objective: The present study was designed to analyze phenolic compounds and essential oil constituents of K . odoratissima fruits. Methods: The n -butanol fraction obtained from hydroalcoholic extract of K. odoratissima fruits was investigated by chromatography on normal phase and Sephadex LH-20 columns. Chemical structures of the isolated compounds were clarified by ¹H-NMR and ¹³C-NMR spectral analyses. Essential oil constituents of the fruits were also analyzed using GC-MS. Results: Phytochemical investigation of the K. odoratissima fruits resulted in the isolation of five flavonol glycosides; isorhamnetin 3-O-glucoside ( 1 ), quercetin 3-O-glucoside (isoquercetin) ( 2 ), isorhamnetin 3-O-rutinoside (narcissin) ( 3 ), isorhamnetin 3-O-glucuronide ( 4 ) and quercetin 3-O-glucuronide (mequilianin) ( 5 ). GC-MS analysis of the fruits essential oil led to the identification of the thirty six compounds, of which ( Z )-ligustilide (15.93 %), δ -cadinene (12.26 %) and germacrene D (12.18 %) were the main compounds. Conclusion: The results of this study introduce K. odoratissima fruits as a source of flavonoid glycosides and phthalate derivatives. The presence of these compounds with known biological properties and health beneficial effects provides more medicinal potentials for the fruits of K. odoratissima and suggest it an appropriate option for further studies.","PeriodicalId":16582,"journal":{"name":"Journal of Medicinal Plants","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44669223","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective: This study was planned to investigate the effects of foliar application of salicylic acid and yeast extract on production of valuable essential oil components in peppermint. Methods: A completely randomized design experiment with nine treatments consisting salicylic acid (40, 80, 160 and 320 mg/l), yeast extract (0.25, 0.75, 1 and 1.5 g/l) and distilled water (control) with three replications was carried out under greenhouse conditions. Results: In total, forty compounds were identified in the essential oils of the plant aerial parts. Menthone, menthol, piperitone, isopulegol and γ -terpinene were the major compounds of the oils studied. Menthone and menthol were 16.69 % and 14.39 % of the essential oils, respectively. Salicylic acid and yeast extract were increased menthone, neomenthol, piperitone, γ -terpinene and isomenthol acetate production 42, 60, 39, 59 and 34 % higher than control plants, respectively. Foliar application with 320 mg/l salicylic acid gave the best result in the enhancement of the major essential oil components of treated plants. The results of correlation between essential oil constituents showed that the neomenthol content had a significant positive correlation with menthone (r = 0.865 ** ), γ -terpinene (r = 0.848 ** ) and negative correlation with isopulegol (r = -0.886 ** ). Conclusion: The quality of essential oil of M. piperita were influenced by the foliar application of salicylic acid and yeast extract at the appropriate concentrations. Elicitation by 320 mg/l salicylic acid was the optimum treatment for menthone, neomenthol, γ -terpinene and piperitone production.
{"title":"Changes in essential oil composition of peppermint (Mentha x piperita L.) affected by yeast extract and salicylic acid foliar application","authors":"Morteza Motiee, M. Abdoli","doi":"10.52547/jmp.20.79.47","DOIUrl":"https://doi.org/10.52547/jmp.20.79.47","url":null,"abstract":"Objective: This study was planned to investigate the effects of foliar application of salicylic acid and yeast extract on production of valuable essential oil components in peppermint. Methods: A completely randomized design experiment with nine treatments consisting salicylic acid (40, 80, 160 and 320 mg/l), yeast extract (0.25, 0.75, 1 and 1.5 g/l) and distilled water (control) with three replications was carried out under greenhouse conditions. Results: In total, forty compounds were identified in the essential oils of the plant aerial parts. Menthone, menthol, piperitone, isopulegol and γ -terpinene were the major compounds of the oils studied. Menthone and menthol were 16.69 % and 14.39 % of the essential oils, respectively. Salicylic acid and yeast extract were increased menthone, neomenthol, piperitone, γ -terpinene and isomenthol acetate production 42, 60, 39, 59 and 34 % higher than control plants, respectively. Foliar application with 320 mg/l salicylic acid gave the best result in the enhancement of the major essential oil components of treated plants. The results of correlation between essential oil constituents showed that the neomenthol content had a significant positive correlation with menthone (r = 0.865 ** ), γ -terpinene (r = 0.848 ** ) and negative correlation with isopulegol (r = -0.886 ** ). Conclusion: The quality of essential oil of M. piperita were influenced by the foliar application of salicylic acid and yeast extract at the appropriate concentrations. Elicitation by 320 mg/l salicylic acid was the optimum treatment for menthone, neomenthol, γ -terpinene and piperitone production.","PeriodicalId":16582,"journal":{"name":"Journal of Medicinal Plants","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44610935","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Anjum, M. Sultan, S. Ferdosh, M. Kaisarul Islam, M. Rashid, L. Nahar, S. Sarker
medicine treatment of various human ailments, liver complications, infections, and headaches. Objective: To carry out phytochemical study, and antimicrobial and free-radical scavenging activity evaluation of E. fusca . Methods: Ground stem bark of this plant was extracted by maceration with methanol, partitioned with various organic solvents, and compounds were isolated by chromatographic means. Structures of isolated compounds were confirmed by spectroscopic analyses. The antibacterial activity was assessed by the disc diffusion method, and the free-radical scavenging activity was determined by DPPH assay. Results: The carbon tetrachloride soluble fraction of the methanol extract of E. fusca afforded shinpterocarpin ( 1 ), lupinifolin ( 2 ), 3,9-dihydroxy-4-(3,3-dimethylallyl) [6a R ,11a R ]-pterocarpan ( 3 ) and β-sitosterol ( 4 ). Compounds 1 - 3 showed considerable antimicrobial activity against five Gram-positive and eight Gram–negative bacterial and three fungal strains tested in this study. Compound 1 exhibited the highest zone of inhibition of 19.4 mm against Bacillus subtilis . Additionally, compounds showed free-radical scavenging effects in DPPH assay with the IC 50 values of 8.8, 7.7 and 7.9 µg/mL for compound 1 , 2 and 3 , respectively. However, they displayed some general toxicity in BSL assay. Conclusion: The isolation of bioactive compounds 1 - 3 supports some traditional medicinal uses of this plant. However, general toxicities found in the BSL assay might raise concerns regarding its safety, while offering a new avenue of future investigation on cytotoxicity of these compounds against human cancer cell lines.
{"title":"Flavonoid, pterocarpans and steroid from Erythrina fusca Lour. growing in Bangladesh: isolation, and antimicrobial and free-radical scavenging activity","authors":"A. Anjum, M. Sultan, S. Ferdosh, M. Kaisarul Islam, M. Rashid, L. Nahar, S. Sarker","doi":"10.52547/jmp.20.79.37","DOIUrl":"https://doi.org/10.52547/jmp.20.79.37","url":null,"abstract":"medicine treatment of various human ailments, liver complications, infections, and headaches. Objective: To carry out phytochemical study, and antimicrobial and free-radical scavenging activity evaluation of E. fusca . Methods: Ground stem bark of this plant was extracted by maceration with methanol, partitioned with various organic solvents, and compounds were isolated by chromatographic means. Structures of isolated compounds were confirmed by spectroscopic analyses. The antibacterial activity was assessed by the disc diffusion method, and the free-radical scavenging activity was determined by DPPH assay. Results: The carbon tetrachloride soluble fraction of the methanol extract of E. fusca afforded shinpterocarpin ( 1 ), lupinifolin ( 2 ), 3,9-dihydroxy-4-(3,3-dimethylallyl) [6a R ,11a R ]-pterocarpan ( 3 ) and β-sitosterol ( 4 ). Compounds 1 - 3 showed considerable antimicrobial activity against five Gram-positive and eight Gram–negative bacterial and three fungal strains tested in this study. Compound 1 exhibited the highest zone of inhibition of 19.4 mm against Bacillus subtilis . Additionally, compounds showed free-radical scavenging effects in DPPH assay with the IC 50 values of 8.8, 7.7 and 7.9 µg/mL for compound 1 , 2 and 3 , respectively. However, they displayed some general toxicity in BSL assay. Conclusion: The isolation of bioactive compounds 1 - 3 supports some traditional medicinal uses of this plant. However, general toxicities found in the BSL assay might raise concerns regarding its safety, while offering a new avenue of future investigation on cytotoxicity of these compounds against human cancer cell lines.","PeriodicalId":16582,"journal":{"name":"Journal of Medicinal Plants","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46350157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
F. T. Talebpour Amiri, F. Mirzaee, Seyedeh Nesa Hasanzadeh, R. Enayatifard, S. Shahani
Background: Proper wound management which improves the quality of life and reduces patient costs is required. Lamium album L. has been used in traditional medicine to heal skin wounds and recent studies show anti-inflammatory, haemostatic and antimicrobial properties of this plant. Objective: The effect of the ointment containing methanol extract of L. album aerial parts was evaluated on the full-thickness wound healing in rat model. Methods: Thirty-two Wistar rats (250-300 g) were used to be created a square full-thickness wound on the dorsal cervical area and randomly divided into four groups: I; control, II; ointment base, III; 1 % phenytoin cream and IV; 5 % L. album ointment. Wound size for determination of the percent of wound healing was measured on days 3, 5, 7 and 12 of the experiment. The excisional biopsies were evaluated histopathologically on the 12 th day of treatment according to the Abramov score method. Results: The herbal ointment significantly increased fibroblast maturation, collagen deposition and neovascularization compared to the control group. The wound healing rate was significantly increased in the group treated with L. album ointment, same as phenytoin group, on days 3, 7 and 12. Based on the spectrophotometric analysis, the extract contains phenol and flavonoid compounds. Conclusion: It seems that L. album could be considered as a new candidate for further studies in the field of wound healing.
背景：需要适当的伤口管理，以提高生活质量并降低患者成本。Lamium album L.在传统医学中被用于治疗皮肤伤口，最近的研究表明这种植物具有抗炎、止血和抗菌的特性。目的：评价木犀地上部分甲醇提取物软膏对大鼠全层创面愈合的影响。方法：32只Wistar大鼠（250～300g）在颈背部形成方形全厚伤口，随机分为四组：I组；对照，II；软膏基质，III；1%苯妥英乳膏和静脉滴注；5%L.相册膏。在实验的第3、5、7和12天测量用于确定伤口愈合百分比的伤口大小。根据Abramov评分法，在治疗第12天对切除的活检进行组织病理学评估。结果：与对照组相比，中药软膏显著增加了成纤维细胞的成熟、胶原沉积和新生血管的形成。在第3、7和12天，L.album软膏治疗组的伤口愈合率显著高于苯妥英钠组。根据分光光度分析，提取物中含有酚类和黄酮类化合物。结论：L.album有望成为创伤愈合领域进一步研究的新候选者。
{"title":"Therapeutic potential of ointment containing methanol extract of Lamium album L. on cutaneous wound healing in rats","authors":"F. T. Talebpour Amiri, F. Mirzaee, Seyedeh Nesa Hasanzadeh, R. Enayatifard, S. Shahani","doi":"10.52547/jmp.20.79.72","DOIUrl":"https://doi.org/10.52547/jmp.20.79.72","url":null,"abstract":"Background: Proper wound management which improves the quality of life and reduces patient costs is required. Lamium album L. has been used in traditional medicine to heal skin wounds and recent studies show anti-inflammatory, haemostatic and antimicrobial properties of this plant. Objective: The effect of the ointment containing methanol extract of L. album aerial parts was evaluated on the full-thickness wound healing in rat model. Methods: Thirty-two Wistar rats (250-300 g) were used to be created a square full-thickness wound on the dorsal cervical area and randomly divided into four groups: I; control, II; ointment base, III; 1 % phenytoin cream and IV; 5 % L. album ointment. Wound size for determination of the percent of wound healing was measured on days 3, 5, 7 and 12 of the experiment. The excisional biopsies were evaluated histopathologically on the 12 th day of treatment according to the Abramov score method. Results: The herbal ointment significantly increased fibroblast maturation, collagen deposition and neovascularization compared to the control group. The wound healing rate was significantly increased in the group treated with L. album ointment, same as phenytoin group, on days 3, 7 and 12. Based on the spectrophotometric analysis, the extract contains phenol and flavonoid compounds. Conclusion: It seems that L. album could be considered as a new candidate for further studies in the field of wound healing.","PeriodicalId":16582,"journal":{"name":"Journal of Medicinal Plants","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45337274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
O. C. Ejelonu, Simeon O. Oluba, Bankole O. Awolokun, O. Elekofehinti, I. G. Adanlawo
Background: Obesity is a medical condition that occurs as a result of excess body fat which increases the risk of several metabolic disorders. Non-availability of efficient classical treatment for obesity has led to propose alternative treatment using plant material. Aim: To investigated the effect of saponin-rich extract of Lindackeria dentata (SLD) on obesity and some specific genes involved in inflammation and insulin resistance in the high-fat diet (HFD) on mice. Setting: The plant leaves were collected from farmland in Igede Ekiti, South-western Nigeria and authenticated at a herbarium unit of the Department of Plant Science and Biotechnology, Ekiti State University, Ado Ekiti, Nigeria. Methods: Saponin-rich extracts from Lindackeria dentata leaves were extracted using standard procedures, HFD was given to some selected mice for 12 weeks whilst monitoring blood glucose and body weight (bw) of the mice. Obese mice were treated orally with SLD 25 mg/kg, 50 mg/kg, 100 mg/kg and 200 mg/kg bw for 3 weeks, sacrificed, organs were collected and some biochemical assays were performed. Expression of some genes and histopathological study of the pancreas were also carried out using a standard scientific protocol. Results: Saponin-rich extract of Lindackeria dentata treatment significantly reduced ( p < 0.05) bw and adipose fat deposit, and caused partial restoration of pancreatic islet expansion (100 mg/kg and 200 mg/kg) coupled with accentuated regulation of leptin, insulin and IL-10 gene (25 mg/kg, 50 mg/kg and 100 mg/kg) when compared with control groups. Conclusion: The present data clearly showed that SLD could be a good intervention in the treatment of obesity and its attendant metabolic disorders.
{"title":"Saponin-rich extracts reverse obesity and offer protection against obesity-induced inflammation in high-fat diet mice","authors":"O. C. Ejelonu, Simeon O. Oluba, Bankole O. Awolokun, O. Elekofehinti, I. G. Adanlawo","doi":"10.4102/JOMPED.V5I1.101","DOIUrl":"https://doi.org/10.4102/JOMPED.V5I1.101","url":null,"abstract":"Background: Obesity is a medical condition that occurs as a result of excess body fat which increases the risk of several metabolic disorders. Non-availability of efficient classical treatment for obesity has led to propose alternative treatment using plant material. Aim: To investigated the effect of saponin-rich extract of Lindackeria dentata (SLD) on obesity and some specific genes involved in inflammation and insulin resistance in the high-fat diet (HFD) on mice. Setting: The plant leaves were collected from farmland in Igede Ekiti, South-western Nigeria and authenticated at a herbarium unit of the Department of Plant Science and Biotechnology, Ekiti State University, Ado Ekiti, Nigeria. Methods: Saponin-rich extracts from Lindackeria dentata leaves were extracted using standard procedures, HFD was given to some selected mice for 12 weeks whilst monitoring blood glucose and body weight (bw) of the mice. Obese mice were treated orally with SLD 25 mg/kg, 50 mg/kg, 100 mg/kg and 200 mg/kg bw for 3 weeks, sacrificed, organs were collected and some biochemical assays were performed. Expression of some genes and histopathological study of the pancreas were also carried out using a standard scientific protocol. Results: Saponin-rich extract of Lindackeria dentata treatment significantly reduced ( p < 0.05) bw and adipose fat deposit, and caused partial restoration of pancreatic islet expansion (100 mg/kg and 200 mg/kg) coupled with accentuated regulation of leptin, insulin and IL-10 gene (25 mg/kg, 50 mg/kg and 100 mg/kg) when compared with control groups. Conclusion: The present data clearly showed that SLD could be a good intervention in the treatment of obesity and its attendant metabolic disorders.","PeriodicalId":16582,"journal":{"name":"Journal of Medicinal Plants","volume":"5 1","pages":"9"},"PeriodicalIF":0.0,"publicationDate":"2021-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48611175","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background: Ellagic acid, a major ellagitannin found in pomegranate extract, might be an attractive natural and safe bioactive compound for prevention of cardiac hypertrophy in many pathological conditions that are associated with elevated circulating angiotensin II (Ang II). Ang II stimulates multiple signal transduction pathways involved in hypertrophy including calcineurin/nuclear factor of activated T cell (NFAT). Objective: The present study aimed to explore the possible anti-hypertrophic activity of ellagic acid against Ang II-induced cardiomyocyte hypertrophy and the role of calcineurin/ NFAT signaling pathway in this action. Methods: H9c2 myocardial cells were treated with different concentrations of ellagic acid one hour before exposure to Ang II. Biological markers of cardiac hypertrophy including changes in cell size and protein content, and atrial natriuretic peptide (ANP) protein expression were assessed using light microscopy, Bradford method and western blotting, respectively. The effects of ellagic acid on the protein expression of calcineurin and nuclear localization of NFATc4 were also investigated using western blotting and immunofluorescence assay, respectively. Results: The results showed that pretreatment with ellagic acid could efficiently prevent Ang II-induced hypertrophic response which was associated with changes in hypertrophy-related biomarkers including increase in cell size and protein content, and ANP overexpression. Moreover, ellagic acid inhibited Ang II-induced calcineurin up-regulation and nuclear localization of NFATc4. Conclusion: In summary, our findings showed that ellagic acid effectively inhibited Ang II-induced cardiomyocyte hypertrophy. This is the first report demonstrating the role of calcineurin/NFAT pathway inhibition in this protective effects. Future in vivo studies are required to elucidate if ellagic acid could ameliorate cardiac hypertrophy and its transition to heart failure.
{"title":"Protective effect of ellagic acid against angiotensin II-induced cardiomyocyte hypertrophy in H9c2 myocardial cells: Role of calcineurin/NFAT pathway","authors":"F. Asadi, A. Razmi, F. Ghazizadeh, M. Shafiei","doi":"10.52547/jmp.20.78.1","DOIUrl":"https://doi.org/10.52547/jmp.20.78.1","url":null,"abstract":"Background: Ellagic acid, a major ellagitannin found in pomegranate extract, might be an attractive natural and safe bioactive compound for prevention of cardiac hypertrophy in many pathological conditions that are associated with elevated circulating angiotensin II (Ang II). Ang II stimulates multiple signal transduction pathways involved in hypertrophy including calcineurin/nuclear factor of activated T cell (NFAT). Objective: The present study aimed to explore the possible anti-hypertrophic activity of ellagic acid against Ang II-induced cardiomyocyte hypertrophy and the role of calcineurin/ NFAT signaling pathway in this action. Methods: H9c2 myocardial cells were treated with different concentrations of ellagic acid one hour before exposure to Ang II. Biological markers of cardiac hypertrophy including changes in cell size and protein content, and atrial natriuretic peptide (ANP) protein expression were assessed using light microscopy, Bradford method and western blotting, respectively. The effects of ellagic acid on the protein expression of calcineurin and nuclear localization of NFATc4 were also investigated using western blotting and immunofluorescence assay, respectively. Results: The results showed that pretreatment with ellagic acid could efficiently prevent Ang II-induced hypertrophic response which was associated with changes in hypertrophy-related biomarkers including increase in cell size and protein content, and ANP overexpression. Moreover, ellagic acid inhibited Ang II-induced calcineurin up-regulation and nuclear localization of NFATc4. Conclusion: In summary, our findings showed that ellagic acid effectively inhibited Ang II-induced cardiomyocyte hypertrophy. This is the first report demonstrating the role of calcineurin/NFAT pathway inhibition in this protective effects. Future in vivo studies are required to elucidate if ellagic acid could ameliorate cardiac hypertrophy and its transition to heart failure.","PeriodicalId":16582,"journal":{"name":"Journal of Medicinal Plants","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70688774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
G. Mosleh, P. Badr, A. Azadi, Forough Afsari Sardari, A. Iraji, Sedigheh Khademian, Z. Abolhassanzadeh, A. Mohagheghzadeh
Abbreviations: DR, Damask rose; GC-MS, Gas chromatography-mass spectrometery; HPLC, High performance liquid chromatography; LOQ, Limits of quantitation; LOD, Limits of detection Background: Damask rose (DR) with the scientific name of Rosa × damascena Herrm. is a popular medicinal plant belonging to the Rosaceae family. According to the literature, DR cultivation was originally developed in the Layzangan valley, Darab region of Fars province in Iran. Objective: In this study, quality control and standardization of Layazangan DR flowers were investigated. Methods: Histological, physicochemical, and heavy metal analysis as well as the chromatographic fingerprints including GC-MS and HPLC analysis were studied. Results: According to the microscopic observations, secretory and simple trichromes in sepal, rosette crystals in ovule, and the tricolporate shape of pollen grain were found to be the major characteristics in DR flowers. The results of heavy metal analysis revealed that the amounts of zinc (82.5 ppm), copper (4.02 ppm), lead (0.11 ppm), and cadmium (not detected) were less than maximum permitted amounts. The essential oil of fresh petals was obtained by hydro-distillation and was analyzed by GC-MS method. Twenty-three components were identified representing 98.66 % of the total essential oil composition. Citronellol (41.44 %), nonadecane (16.44 %), and heneicosane (10.58 %) were the main components. According to HPLC analysis results, quercetin was determined as 7300.5 μg/g dried DR petals. Conclusion: The essential oil obtained from Layzangan DR is rich in citronellol compound. On the other hand, the diagnostic characteristics presented in this study can help in better quality control and standardization of DR samples. It could also provide information for the authentication of relevant undeclared samples as well as the detection of adulterated materials.
{"title":"Quality control of Rosa × damascena flowers from Layzangan of Fars province in Iran","authors":"G. Mosleh, P. Badr, A. Azadi, Forough Afsari Sardari, A. Iraji, Sedigheh Khademian, Z. Abolhassanzadeh, A. Mohagheghzadeh","doi":"10.52547/jmp.20.78.48","DOIUrl":"https://doi.org/10.52547/jmp.20.78.48","url":null,"abstract":"Abbreviations: DR, Damask rose; GC-MS, Gas chromatography-mass spectrometery; HPLC, High performance liquid chromatography; LOQ, Limits of quantitation; LOD, Limits of detection Background: Damask rose (DR) with the scientific name of Rosa × damascena Herrm. is a popular medicinal plant belonging to the Rosaceae family. According to the literature, DR cultivation was originally developed in the Layzangan valley, Darab region of Fars province in Iran. Objective: In this study, quality control and standardization of Layazangan DR flowers were investigated. Methods: Histological, physicochemical, and heavy metal analysis as well as the chromatographic fingerprints including GC-MS and HPLC analysis were studied. Results: According to the microscopic observations, secretory and simple trichromes in sepal, rosette crystals in ovule, and the tricolporate shape of pollen grain were found to be the major characteristics in DR flowers. The results of heavy metal analysis revealed that the amounts of zinc (82.5 ppm), copper (4.02 ppm), lead (0.11 ppm), and cadmium (not detected) were less than maximum permitted amounts. The essential oil of fresh petals was obtained by hydro-distillation and was analyzed by GC-MS method. Twenty-three components were identified representing 98.66 % of the total essential oil composition. Citronellol (41.44 %), nonadecane (16.44 %), and heneicosane (10.58 %) were the main components. According to HPLC analysis results, quercetin was determined as 7300.5 μg/g dried DR petals. Conclusion: The essential oil obtained from Layzangan DR is rich in citronellol compound. On the other hand, the diagnostic characteristics presented in this study can help in better quality control and standardization of DR samples. It could also provide information for the authentication of relevant undeclared samples as well as the detection of adulterated materials.","PeriodicalId":16582,"journal":{"name":"Journal of Medicinal Plants","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70688795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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