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Medicinal Plants: Domestication, Biotechnology and Regional Importance 药用植物:驯化、生物技术和区域重要性
Q3 Medicine Pub Date : 2021-01-01 DOI: 10.1007/978-3-030-74779-4
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引用次数: 5
Evaluating the quality of commercial Iranian honeys 评价伊朗商业蜂蜜的质量
Q3 Medicine Pub Date : 2021-01-01 DOI: 10.52547/jmp.20.78.14
F. Nabati, F. Khalighi-Sigaroodi, M. Kashefi, Seyed Vahid Ghasemi, Hadis Sadri, F. Tajabadi
Background: Honey is one of the most valuable foods that used in human societies to treat many diseases due to its healing properties for centuries. The lack of an objective way to distinguish natural honey from counterfeit honey has strengthened the field of profiteering in this area and has led to the supply of counterfeit honey instead of natural honey. So honey quality must be controlled analytically with the aim of guaranteeing the reality and preserving the consumer from commercial speculation. Objective: The present study was conducted to evaluate 24 samples of 9 commercial brands of honey in Tehran’s markets with number 1 to 9 (1-AB, 2-TF, 3-DP, 4-JK, 5-SN, 6-SK, 7-IM, 8-MD, and 9-MH). A number of physical, chemical, and biological parameters of the samples were evaluated. Methods: Carbohydrate composition was determined by GC/Mass to evaluate the contents of fructose, glucose, and sucrose. 5-hydroxymethyl-2-furfuraldehyde (HMF) was quantified by HPLC-UV and other physicochemical quality parameters including moisture, pH, proline content; and diastase activity were also evaluated according to the Codex Alimentarius. Results: Only 2 brands met all major international specifications. Although all the parameters of the other brands were conformed, the diastase activity was not in a specific range. Conclusion: The diastasis activity is considered as the most important factor to evaluate honey quality based on the results of this study.
背景:蜂蜜是人类社会中最有价值的食物之一,由于其治疗特性,几个世纪以来一直用于治疗许多疾病。由于缺乏客观的方法来区分天然蜂蜜和假冒蜂蜜,这加强了这一领域的暴利,导致假冒蜂蜜代替天然蜂蜜的供应。因此,必须对蜂蜜质量进行分析控制,以保证蜂蜜质量的真实性,避免消费者受到商业炒作的影响。目的:本研究对德黑兰市场上9个商业品牌(1- ab、2-TF、3-DP、4-JK、5-SN、6-SK、7-IM、8-MD和9- mh)的24个样品进行了评价。对样品的一些物理、化学和生物学参数进行了评估。方法:采用GC/Mass法测定糖的组成,测定果糖、葡萄糖和蔗糖的含量。5-羟甲基-2-糠醛(HMF)采用HPLC-UV及其他理化质量参数(水分、pH、脯氨酸含量)定量;并根据《食品法典》评价淀粉酶活性。结果:只有2个品牌符合所有主要国际标准。虽然其他品牌的所有参数都符合,但淀粉酶活性不在一个特定的范围内。结论:在本研究的基础上,认为蜂蜜的挥发活性是评价蜂蜜品质的最重要因素。
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引用次数: 3
Effect of salicylic acid and yeast extract on caffeic acid derivatives production in Echinacea purpurea L. 水杨酸和酵母提取物对紫锥菊咖啡酸衍生物生产的影响。
Q3 Medicine Pub Date : 2021-01-01 DOI: 10.52547/jmp.20.78.36
M. Abdoli, Zhila Mehrpooya, Mohammad Reza Talebian
Background: Echinacea has been used in remedies to prevent and a wide range of diseases like common cold, simple cough, pulmonary infections, dermatologic disorder and chronic diseases due to immunodeficiency. Objective : The present study was conducted to investigate the effects of salicylic acid and yeast extract foliar application on caffeic acid derivatives production in coneflower. Methods: Two factorial experiments were performed individually under greenhouse conditions during the years 2017-2018. In the first experiment, the effect of salicylic acid (0, 80, 160 and 320 mg/L) and each at four exposure times (24, 48, 72 and 96 hours) was studied. Also, in the second experiment, the effect of yeast extract (0, 0.75, 1 and 1.5 g/L) and each at four exposure times was investigated. Results: The results indicated that main effects and interactions of different concentrations of salicylic acid and yeast extract at different exposure times on caffeic acid derivatives content were significant (P ˂ 0.01). The highest cichoric acid, caftaric acid and echinacoside production was obtained after 96 hours of treatment at 160 mg/L salicylic acid treated plants. The highest cichoric acid production 7.150 mg/g DW was obtained after 96 hours of treatment at 1.5 g/L yeast extract treated plants, this was 3.58-fold higher compared to respective control. The lowest level of caffeic acid derivatives production was observed in control plants. Conclusion: In conclusion, salicylic acid and yeast extract foliar application have a positive effect on caffeic acid derivatives production in Echinacea purpurea aerial parts.
背景:紫锥菊已被用于治疗和预防广泛的疾病,如普通感冒,单纯性咳嗽,肺部感染,皮肤病和慢性疾病由于免疫缺陷。目的:研究水杨酸和酵母浸膏叶面施用对牛角花中咖啡酸衍生物生产的影响。方法:2017-2018年在温室条件下分别进行两项析因试验。在第一个实验中,研究了水杨酸(0、80、160和320 mg/L)和分别在4个暴露时间(24、48、72和96 h)下的影响。在第二个实验中,研究了酵母浸膏(0、0.75、1和1.5 g/L)和每种酵母浸膏在4次暴露下的效果。结果:不同浓度水杨酸和酵母浸膏不同暴露时间对咖啡酸衍生物含量的主效应和交互作用显著(P小于0.01)。160 mg/L水杨酸处理96 h后,菊苣酸、果酸和紫锥菊苷的产量最高。1.5 g/L酵母浸膏处理96 h后,菊苣酸产量最高,为7.150 mg/g DW,是对照的3.58倍。对照植株咖啡酸衍生物的产量最低。结论:叶面施用水杨酸和酵母浸出物对紫锥菊地上部位咖啡酸衍生物的生成有积极影响。
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引用次数: 4
Some aspects of the adaptogenic potential of European mistletoe (Viscum album L.) extracts under variable physical performance 不同物理性能下欧洲槲寄生(Viscum album L.)提取物适应潜力的一些方面
Q3 Medicine Pub Date : 2021-01-01 DOI: 10.52547/JMP.20.77.60
D. Pozdnyakov, S. Adzhiahmetova, N. Chervonnaya, A. Voronkov, E. Oganesyan
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引用次数: 5
Regulation effects of Trigonella foenum-graecum seed extract on a mouse model of allergic asthma 葫芦巴种子提取物对小鼠变应性哮喘模型的调节作用
Q3 Medicine Pub Date : 2021-01-01 DOI: 10.52547/jmp.20.78.26
S. Athari, M. Keshavarz, Mahmood Khodadoost, M. Hamzeloo-Moghadam, A. Abbassian, R. Choopani
Background: Trigonella foenum-graecum L. (fenugreek) seeds have a long history of treating allergic asthma known as “Rabv” in Persian medicine. The protective effects of fenugreek on asthma have been noted in Persian medicine texts. Objective : The aim of the present study was to investigate the protective effects of aqueous fenugreek seed extract (AFSE) on the reduction of Th2 cytokines via decreasing the levels of mRNA expression of Th2 cytokines in bronchoalveolar lavage fluid (BALF). Methods: 28 female Balb/c mice were divided into four groups of seven animals. Negative and positive control groups received Phosphate-buffered saline and ovalbumin (OVA), respectively. The remaining two groups were firstly sensitized with OVA to induce asthma and then received Theophylline and AFSE. Thereafter, the mRNA expressions of Th2-type interleukins (IL-4, IL-5, and IL-13) and mucin5 as well as the concentrations of IL-5, IL-13, and IL-33 in BALF samples were measured and pathological alterations of lung tissues were analyzed. Results : AFSE treatment of Balb/c mice significantly decreased the number of eosinophils, the mRNA expressions of IL-4, IL-5, IL-13, and mucin5 and the concentrations of IL-5, IL-13, and IL33 in their BALF specimens. It also considerably prevented peribronchial and perivascular inflammations, mucus hypersecretion, and goblet cell hyperplasia in lung tissues in comparison to OVA-sensitized mice. Conclusion : The present study demonstrated that the aqueous fenugreek seeds extract could be an alternative medication for the treatment of allergic asthma.
背景:葫芦巴种子具有治疗过敏性哮喘的悠久历史,在波斯医学中被称为“Rabv”。葫芦巴对哮喘的保护作用已经在波斯医学文献中被注意到。目的:探讨胡芦巴种子水提物(AFSE)通过降低支气管肺泡灌洗液(BALF)中Th2细胞因子mRNA表达水平,对Th2细胞因子减少的保护作用。方法:28只雌性Balb/c小鼠分为4组,每组7只。阴性对照组和阳性对照组分别给予磷酸盐缓冲盐水和卵清蛋白(OVA)。其余两组先用OVA致敏诱导哮喘,再用茶碱和AFSE。检测BALF样品中th2型白细胞介素(IL-4、IL-5、IL-13)、mucin5 mRNA表达及IL-5、IL-13、IL-33浓度,分析肺组织病理改变。结果:AFSE处理Balb/c小鼠BALF标本中嗜酸性粒细胞数量、IL-4、IL-5、IL-13、mucin5 mRNA表达及IL-5、IL-13、il - 33浓度显著降低。与ova致敏小鼠相比,它还能显著防止支气管周围和血管周围炎症、粘液分泌过多和肺组织杯状细胞增生。结论:胡芦巴种子水提物可作为治疗过敏性哮喘的替代药物。
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引用次数: 0
Polyherbal tablet based on Iranian traditional medicine 以伊朗传统医药为基础的复方草药片
Q3 Medicine Pub Date : 2021-01-01 DOI: 10.52547/JMP.20.77.15
Maedeh Rezghi, S. Mortazavi, S. Fahimi, R. Choopani, Abbas Sheikholeslam, M. Hamzeloo-Moghadam
Background: Medicinal plants have important role for prevention and treatment of diseases regarding their traditional uses. In Iranian traditional medicine (ITM), herbal medicines have been considered as the key agents in the treatment of wide ranges of disorders. Traditional preparations can be reformulated to achieve pharmacopoeia standards as modern medicines. Objective: In this research, a polyherbal tablet namely “Monzej-e-balgham”, which is widely used for treating different diseases, has been formulated and quality control assessments done to present a proper formulation. Methods: Onopordum and Foeniculum Mill. were extracted with water, filtered, concentrated and dried with spray drying method. Thirteen tablets formulations by direct compression and pre- and post- formulation analyses were prepared. Finally, the most proper formulation was selected and evaluated physicochemically and microbiologically according to standard protocols. The total phenol content as marker of the tablets was determined and accelerated stability tests were conducted (40 °C/75 % RH) as per ICH guidelines for six months. Results: For the selected formulation (F2), the hardness, weight variation, friability, disintegration time and drug release were determined as 6.78 ± 0.28 kg/cm 2 , 714.36 ± 1.71 mg, 0.79 ± 0.05 %, 10.18 ± 0.39 min and 97.67 ± 0.16% in one hour, respectively. The amount of total phenol was found to be 25.15 ± 0.13 mg/tablet. Microbial levels were in agreement with requirements. In the accelerated stability tests, no significant changes were observed. Total phenol content decreased 1.03 % during 6 months. Conclusion: After completing complementary tests such as in vivo and clinical trials, the formulated tablet (F2) could be presented for mass production.
背景:药用植物在传统用途方面对疾病的预防和治疗具有重要作用。在伊朗传统医学中,草药被认为是治疗各种疾病的关键药物。传统制剂可以重新配制,以达到现代药物的药典标准。目的:本研究制备了一种广泛用于治疗不同疾病的多羟基片“Monzej-e-balgham”,并进行了质量控制评估,以提供合适的处方。方法:小茴香和小茴香。用水提取,过滤,浓缩,喷雾干燥。通过直接压片和制剂前后分析制备了13种片剂制剂。最后,选择了最合适的配方,并根据标准方案对其进行了物理化学和微生物学评估。测定了作为片剂标志的总酚含量,并根据ICH指南进行了为期六个月的加速稳定性测试(40°C/75%RH)。结果:所选制剂(F2)在1小时内的硬度、重量变化、脆性、崩解时间和药物释放度分别为6.78±0.28kg/cm2、714.36±1.71mg、0.79±0.05%、10.18±0.39min和97.67±0.16%。总酚含量为25.15±0.13mg/片。微生物水平符合要求。在加速稳定性试验中,未观察到显著变化。总酚含量在6个月内下降了1.03%。结论:在完成体内试验和临床试验等补充试验后,该制剂(F2)可以进行大规模生产。
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引用次数: 3
Essential oil bioactivity evaluation of the different populations of Cupressus against adult rice weevil (Sitophilus oryzae L.) 不同Cupressus种群对稻象甲成虫的精油生物活性评价
Q3 Medicine Pub Date : 2021-01-01 DOI: 10.52547/jmp.20.77.79
M. Labbafi, M. Ahvazi, F. Khalighi-Sigaroodi, H. Khalaj, S. Ahmadian, F. Tajabadi, M. Khani, S. Amini
Background: A significant amount of crop production is destroyed annually by pests in the storage and significant damage is caused to crops. Recently, the use of essential oils as an alternative to pesticides in pest control has been considered. Due to the presence of α -pinene, Cupressus genus is a significant source of plant insecticides that can act as fumigant, repellency and inhibitory insecticides. Objective: The aim of this study was to investigate the insecticidal properties, repellency and nutritional parameters of essential oils of Cupressus species on Sitophilus oryzae in 2018. Methods: The experiment was conducted in a factorial arrangement based on completely randomized design with 4 replications. The essential oils of the different species and populations of Cupressus were prepared by the Clevenger apparatus using a water distillation method. The major constituents in the essential oils were analyzed by GC-MS and the biological effects of the essential oils against adult S. oryzae were investigated. Results: In the analysis of chemical compounds, α -pinene was identified as the main essential oils of the above species. The result showed that among the species, C. arizonica showed the highest Fumigant toxicity to S . oryzae with LC 50 = 172.30 μl/L air. In investigating the effect of repellency on S. oryzae, essential oils of C. sempervirens L. var. horizontalis (France) with 80.61 % showed the highest distance. Conclusion: Due to the toxicity and high repellency effect of Cupressus essential oils on S. oryzae , this genus has high potential for use in storage pest control.
背景:每年有大量的作物生产被储存中的害虫破坏,并对作物造成重大损害。最近,人们考虑在害虫防治中使用精油作为杀虫剂的替代品。由于α-蒎烯的存在,柏属是植物杀虫剂的重要来源,可作为熏蒸剂、驱虫剂和抑制性杀虫剂。目的:本研究旨在研究2018年白柏属植物精油对米象的杀虫特性、驱避性和营养参数。方法:实验采用完全随机设计的析因安排,4次重复。采用Clevenger装置,采用水蒸馏法,制备了不同种类和种群的柏精油。采用气相色谱-质谱联用技术对其主要成分进行了分析,并对其对稻瘟病成虫的生物学效应进行了研究。结果:在化学成分分析中,α-蒎烯被鉴定为上述物种的主要精油。结果表明,在这些物种中,亚利桑那C.arizonica对S的熏蒸剂毒性最高。用LC50=172.30μ。在研究驱避性对米曲霉的影响时,法国产的C.sempervirens L.var.horizontalis精油的驱避性距离最高,达80.61%。结论:柏精油对米曲霉具有较高的驱避性和毒性,具有很高的贮藏害虫防治潜力。
{"title":"Essential oil bioactivity evaluation of the different populations of Cupressus against adult rice weevil (Sitophilus oryzae L.)","authors":"M. Labbafi, M. Ahvazi, F. Khalighi-Sigaroodi, H. Khalaj, S. Ahmadian, F. Tajabadi, M. Khani, S. Amini","doi":"10.52547/jmp.20.77.79","DOIUrl":"https://doi.org/10.52547/jmp.20.77.79","url":null,"abstract":"Background: A significant amount of crop production is destroyed annually by pests in the storage and significant damage is caused to crops. Recently, the use of essential oils as an alternative to pesticides in pest control has been considered. Due to the presence of α -pinene, Cupressus genus is a significant source of plant insecticides that can act as fumigant, repellency and inhibitory insecticides. Objective: The aim of this study was to investigate the insecticidal properties, repellency and nutritional parameters of essential oils of Cupressus species on Sitophilus oryzae in 2018. Methods: The experiment was conducted in a factorial arrangement based on completely randomized design with 4 replications. The essential oils of the different species and populations of Cupressus were prepared by the Clevenger apparatus using a water distillation method. The major constituents in the essential oils were analyzed by GC-MS and the biological effects of the essential oils against adult S. oryzae were investigated. Results: In the analysis of chemical compounds, α -pinene was identified as the main essential oils of the above species. The result showed that among the species, C. arizonica showed the highest Fumigant toxicity to S . oryzae with LC 50 = 172.30 μl/L air. In investigating the effect of repellency on S. oryzae, essential oils of C. sempervirens L. var. horizontalis (France) with 80.61 % showed the highest distance. Conclusion: Due to the toxicity and high repellency effect of Cupressus essential oils on S. oryzae , this genus has high potential for use in storage pest control.","PeriodicalId":16582,"journal":{"name":"Journal of Medicinal Plants","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46259086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 5
The efficient method for in vitro micropropagation of Ginkgo biloba L. 银杏离体微繁的有效方法。
Q3 Medicine Pub Date : 2021-01-01 DOI: 10.52547/jmp.20.78.78
A. Qaderi, A. Mehrafarin, S. Rezazadeh, N. Zarinpanjeh
Background : Ginkgo biloba L. leaf extract is used in medicine due to its therapeutic actions such as regulating cerebral blood flow, lowering oxidative stress, delaying the progress of dementia and diabetes. Objective: At the current study, the efficient micropropagation of G. biloba has been optimized by applying tissue culture method. Methods: Different explants (leaves, stems and lateral buds) were disinfected superficially and were cultured in WPM supplemented with various types and concentrations of plant growth regulators for shooting and subsequently for root induction. Results: The best treatment for shoot induction frequency (100 %), regenerated shoot length (2.47 cm) and number of regenerated leaves per explant (6.5) was achieved by culturing the lateral buds on WPM medium having Kin at 1 mg/L and IAA at 0.5 mg/L. The best root induction medium on the basis of root induction (100 %) and regenerated root lengths (8.5 cm) was WPM medium with IBA at 1 mg/L and AC at 2 g/L. After acclimatization, 60 % of regenerated platelets were survived. Finally, based on HPLC analysis, no significant difference was observed between the amount of quercetin in the leaves of propagated seedlings under in vitro conditions and their mother base. Conclusion: The optimized protocol proposed to be used as an efficient method for commercial micropropagation of ginkgo tree.
背景:银杏叶提取物具有调节脑血流、降低氧化应激、延缓痴呆和糖尿病进展等治疗作用,被广泛应用于医药领域。目的:本研究采用组织培养的方法,对双歧杆菌的高效增殖进行了优化。方法:对不同外植体(叶、茎和侧芽)进行表面消毒,在添加不同类型和浓度的植物生长调节剂的WPM中培养,然后进行生根诱导。结果:在Kin浓度为1 mg/L、IAA浓度为0.5 mg/L的WPM培养基上培养侧芽,获得了芽诱导频率(100%)、再生芽长(2.47 cm)和每外植体再生叶数(6.5)的最佳处理。以诱导率(100%)和再生根长(8.5 cm)为基础,WPM培养基中IBA浓度为1 mg/L, AC浓度为2 g/L。经驯化后,再生血小板成活率为60%。最后,通过HPLC分析,离体条件下繁殖苗叶片中槲皮素的含量与母本无显著差异。结论:该优化方案可作为银杏商业微繁的有效方法。
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引用次数: 1
Formulation and quality evaluation of Coriander Triphala tablet 芫荽三甲片的处方及质量评价
Q3 Medicine Pub Date : 2021-01-01 DOI: 10.52547/jmp.20.78.68
R. Choopani, H. Hajimehdipoor, K. Molaei, Haniye Kashafroodi, Fatemeh Tavakolifar, L. Ara
Background: Coriander Triphala is one of the famous drugs in traditional medicine which is consisted of Terminalia chebula , T. bellirica , Phyllanthus emblica , Coriandrum sativum , almond oil and honey. Traditional dosage forms should be converted to modern forms for better acceptance and suitable characteristics and stability. Objective: In the present investigation, the traditional form of Coriander Triphala was converted to film coated tablet
背景:芫荽是一种著名的传统药物,由苦参、苦参、余甘子、芫荽、杏仁油和蜂蜜组成。传统剂型应转换为现代剂型,以更好地接受和适合的特性和稳定性。目的:将香菜传统剂型转化为薄膜包衣片
{"title":"Formulation and quality evaluation of Coriander Triphala tablet","authors":"R. Choopani, H. Hajimehdipoor, K. Molaei, Haniye Kashafroodi, Fatemeh Tavakolifar, L. Ara","doi":"10.52547/jmp.20.78.68","DOIUrl":"https://doi.org/10.52547/jmp.20.78.68","url":null,"abstract":"Background: Coriander Triphala is one of the famous drugs in traditional medicine which is consisted of Terminalia chebula , T. bellirica , Phyllanthus emblica , Coriandrum sativum , almond oil and honey. Traditional dosage forms should be converted to modern forms for better acceptance and suitable characteristics and stability. Objective: In the present investigation, the traditional form of Coriander Triphala was converted to film coated tablet","PeriodicalId":16582,"journal":{"name":"Journal of Medicinal Plants","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70689353","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Improving biochemical traits, oleo-gum yield and compositions of asafoetida (Ferula assa-foetida L.) essential oil using 24-epibrassinolide in Kerman natural habitats (Iran) 利用24-表油菜素内酯改善伊朗Kerman自然生境阿魏精油生化特性、油胶产量和成分
Q3 Medicine Pub Date : 2021-01-01 DOI: 10.52547/JMP.20.77.93
A. Saadatfar, S. Jafari, I. Tavassolian
Background: Asafoetida ( Ferula assa-foetida L.) is an endangered endemic plant which is widely used in food, cosmetics and pharmaceutical industries. It is necessary to enhance the quantity and quality of its secondary metabolites in order to meet requirements of the industries. Objective: This study investigated the impact of 24-epibrassinolide (EBL) on biochemical traits, essential oil yield and chemical compositions of Ferula assa-foetida in its natural habitat. Methods: We foliar sprayed the EBL at the concentrations of 0, 0.1, 0.5 and 1 µM with three times during a week at fully matured leaves phase. One month after treatments application, leaf sampling and gum gathering of the plants was done and the mentioned parameters were measured. Results: ANOVA results indicated that 1 µM EBL showed the best results for chlorophyll a, b, total chlorophyll, carotenoids, reduced sugar and protein contents (17.77, 13.08, 31.57, 7.50, 40.45 and 0.34 mg/g FW). The main components of the essential oil are α -pinene (8.82 %), β -pinene (11.57 %) and myrcene (1.12 %) that showed a significant increase at 1 µM of EBL. Although, EBL at 0.5 µM was proved to be the best treatment for leaf total phenol content (79.37 mg/g FW) and sulfurous compounds such as: (E)- 1-propenyl sec-butyl disulfide (51.48 %), bis (1-methyl propyl) disulfide (0.9 %) and n- propyl sec-butyl disulfide (0.41 %). The highest amount of flavonoids (146.18; 162.56 mg/g FW) and essential oil yield (7.91 %; 8.16 %) were obtained at 0.5 and 1 µM EBL concentrations, respectively compared to the control (6 %). Conclusion: Our results indicated the promising and positive effects of EBL, as an environmental friendly strategy, to improve oleo-gum quantity and quality.
背景:阿魏(Ferula assa-foetida L.)是一种濒危的地方植物,广泛应用于食品、化妆品和制药行业。提高其次生代谢产物的数量和质量,以满足工业生产的需要。目的:研究24-表油菜素内酯(EBL)对阿魏(assa-foetida)自然生境生化性状、精油产量和化学成分的影响。方法:采用叶面喷施浓度分别为0、0.1、0.5、1µM的EBL,每周喷施3次。施用1个月后,对植株进行叶片取样和树胶采集,并对上述参数进行测定。结果:方差分析结果表明,1µM EBL处理对叶绿素a、b、总叶绿素、类胡萝卜素、还原糖和蛋白质含量的影响最佳(17.77、13.08、31.57、7.50、40.45和0.34 mg/g FW)。挥发油的主要成分为α -蒎烯(8.82%)、β -蒎烯(11.57%)和月桂烯(1.12%),在EBL浓度为1µM时显著升高。结果表明,0.5µM EBL处理对叶片总酚含量(79.37 mg/g FW)和含硫化合物(E)- 1-丙基仲丁基二硫(51.48%)、二(1-甲基丙基)二硫(0.9%)和正丙基仲丁基二硫(0.41%))的影响最大。黄酮类化合物含量最高(146.18;162.56 mg/g FW)和精油得率(7.91%;0.5µM和1µM EBL浓度下,与对照组(6%)相比,分别为8.16%。结论:本研究结果表明,EBL作为一种环境友好策略,在提高油胶数量和质量方面具有良好的前景和积极的作用。
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引用次数: 2
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Journal of Medicinal Plants
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