Journal of Oral Pathology & Medicine最新文献

{"title":"Can Isotretinoin Induce Cytogenetic Damage in Oral Cells?","authors":"Thiago Guedes Pinto, Wilton Mitsunari Takeshita, Patricia Ramos Cury, Jean Nunes dos Santos, Daniel Araki Ribeiro","doi":"10.1111/jop.70077","DOIUrl":"10.1111/jop.70077","url":null,"abstract":"","PeriodicalId":16588,"journal":{"name":"Journal of Oral Pathology & Medicine","volume":"55 2","pages":"175-176"},"PeriodicalIF":2.3,"publicationDate":"2025-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145488886","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Therapeutic Potential of Cannabidiol in Dentistry: A Systematic Review From Cellular Mechanisms to Clinical Trials","authors":"Chatvadee Kornsuthisopon,&nbsp;Ajjima Chansaenroj,&nbsp;Sornkanok Vimolmangkang,&nbsp;Lakshman P. Samaranayake,&nbsp;Thanaphum Osathanon","doi":"10.1111/jop.70081","DOIUrl":"10.1111/jop.70081","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>CBD holds substantial promise in medical applications. This review aims to comprehensively analyse the current status of cannabidiol (CBD) in dentistry.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>A systematic search of databases including PubMed-MEDLINE, Scopus, Embase, Cochrane Library, World Intellectual Property Organization (WIPO), European Patent Office (EPO), and the United States Patent and Trademark Office (USPTO) was conducted. Peer-reviewed journal manuscripts focusing on cell studies, clinical trials, and registered patents related to CBD and its derivatives in dentistry were summarised. Inclusion criteria were studies on CBD in dentistry, including original research and patents, published in English between 2013 and mid-2023 (articles) or early 2024 (patents), with full-text availability. Excluded were non-dentistry studies, unpublished or non-peer-reviewed reports, and duplicates using Microsoft Excel. The risk of bias was evaluated using the Cochrane RoB 2 tool. Two observers independently screened the articles for inclusion in the present study to mitigate bias. Cohen's kappa was used to measure inter-rater agreement.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>The total number of included studies was 57. Cell-based studies demonstrated CBD's effectiveness in modulating cellular responses and anti-inflammatory properties, especially in oral-origin cells, and its impact on osteogenic differentiation. Research, including clinical trials and patents, has shown CBD's benefits in treating pain and inflammation in the maxillofacial area, notably in conditions such as radiation-induced mucositis. CBD research in dental pain and inflammation is advanced, but studies on CBD's role in regenerative dentistry remain limited.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>More studies on the mineralisation of oro-facial structures are necessary to fully understand CBD's role in regenerative dentistry. This study was supported by the Faculty of Dentistry, Chulalongkorn University. This study was registered in the PROSPERO (ID: CRD4201055832) and Open Science Framework (OSF) database (osf.io/z3bd8). The PRISMA guideline was followed to include the relevant full-text papers.</p>\u0000 </section>\u0000 </div>","PeriodicalId":16588,"journal":{"name":"Journal of Oral Pathology & Medicine","volume":"55 2","pages":"177-190"},"PeriodicalIF":2.3,"publicationDate":"2025-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145451653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ZNF582 Inhibits Head and Neck Squamous Cell Carcinoma Progression and Stemness via MAPK and PI3K/AKT Signaling Pathways","authors":"Yaqing Mao,&nbsp;Shuo Liu,&nbsp;Zhen Qin,&nbsp;Wenbo Zhang,&nbsp;Lingfei Jia,&nbsp;Xin Peng","doi":"10.1111/jop.70079","DOIUrl":"10.1111/jop.70079","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Head and neck squamous cell carcinoma (HNSCC) is an aggressive malignancy characterized by high metastatic potential and poor prognosis. Previous studies have focused on the correlation between hypermethylated ZNF582 and HNSCC prognosis, particularly in diagnostic contexts, but the underlying molecular mechanisms remain poorly understood. This study explored the biological function and underlying mechanisms of ZNF582 in HNSCC.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>ZNF582 expression was analyzed in HNSCC and non-tumor tissues. Functional assays, including colony formation, wound healing, Transwell, TUNEL assays, and xenograft models, were performed in HNSCC cell lines with or without ZNF582 overexpression. To assess cancer stemness, fluorescence-activated cell sorting, tumorsphere formation assay, extreme limiting dilution assay, and an orthotopic model were used. High-throughput RNA sequencing was performed to identify the altered signaling pathways.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Bioinformatics analysis revealed that low ZNF582 expression in HNSCC correlated with poor prognosis. Overexpression of ZNF582 suppressed HNSCC proliferation, migration, and invasion, while promoting apoptosis both in vitro and in vivo. ZNF582 also reduced cancer stem cell activity and metastasis in HNSCC. Mechanically, ZNF582 overexpression inhibited the MAPK and PI3K/AKT signaling pathways.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>Our findings suggested that ZNF582 overexpression inhibits HNSCC progression via the MAPK and PI3K/AKT signaling pathways, highlighting <i>ZNF582</i> as a potential therapeutic target gene for HNSCC treatment.</p>\u0000 </section>\u0000 </div>","PeriodicalId":16588,"journal":{"name":"Journal of Oral Pathology & Medicine","volume":"55 2","pages":"219-231"},"PeriodicalIF":2.3,"publicationDate":"2025-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145451716","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CIAPIN1 as a Promoter of Oral Squamous Cell Carcinoma Progression","authors":"Shan Yang,&nbsp;Changsheng Sun,&nbsp;Zhikai Fang,&nbsp;Hui Jin,&nbsp;Chang Li,&nbsp;Hongfeng Zang,&nbsp;Liu Yang,&nbsp;Lili Li,&nbsp;Wuliji Saiyin","doi":"10.1111/jop.70083","DOIUrl":"10.1111/jop.70083","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objective</h3>\u0000 \u0000 <p>This study aims to investigate the relationship between the expression of cytokine-induced apoptosis inhibitor 1 (CIAPIN1) and the development of oral squamous cell carcinoma (OSCC).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Materials and Methods</h3>\u0000 \u0000 <p>Bioinformatics analyzed CIAPIN1 in pan-cancers; IHC detected CIAPIN1 expression in OSCC and healthy samples; qRT-PCR and WB assayed CIAPIN1 mRNA and protein levels in OSCC and normal cells; MTT, scratch, transwell, flow cytometry, and WB evaluated OSCC cell viability, migration, invasion, EMT, and apoptosis after CIAPIN1 knockdown.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>CIAPIN1 abnormally expressed in multiple tumors, especially OSCC, is linked to prognosis. IHC showed elevated CIAPIN1 in OSCC tissues (<i>p</i> &lt; 0.001). qRT-PCR and WB results showed CIAPIN1 was upregulated in CAL-27 (qRT-PCR: <i>p</i> &lt; 0.05, WB: <i>p</i> &lt; 0.05), SAS (qRT-PCR: <i>p</i> &lt; 0.05, WB: <i>p</i> &lt; 0.01), and SCC9 (qRT-PCR: <i>p</i> &lt; 0.01, WB: <i>p</i> &lt; 0.001) cells compared to HOK cells. After the knockdown of CIAPIN1 in SAS and SCC9 cells, MTT assays revealed reduced cell viability (SAS: <i>p</i> &lt; 0.001, SCC9: <i>p</i> &lt; 0.001); scratch tests showed suppressed migration ability (SAS: <i>p</i> &lt; 0.01, SCC9: <i>p</i> &lt; 0.001); transwell assays indicated inhibited invasion capability (SAS: <i>p</i> &lt; 0.01, SCC9: <i>p</i> &lt; 0.001); flow cytometry revealed increased apoptosis rates (SAS: <i>p</i> &lt; 0.01, SCC9: <i>p</i> &lt; 0.01); WB analysis showed reduced N-cadherin expression and increased E-cadherin expression (SAS for N-cadherin: <i>p</i> &lt; 0.05, SCC9 for N-cadherin: <i>p</i> &lt; 0.01; SAS for E-cadherin: <i>p</i> &lt; 0.01, SCC9 for E-cadherin: <i>p</i> &lt; 0.05), indicating CIAPIN1 promotes EMT.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>CIAPIN1 contributes to the malignant progression of oral squamous cell carcinoma.</p>\u0000 </section>\u0000 </div>","PeriodicalId":16588,"journal":{"name":"Journal of Oral Pathology & Medicine","volume":"55 2","pages":"209-218"},"PeriodicalIF":2.3,"publicationDate":"2025-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145445165","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"COVID-19 Dry Mouth Induced by SARS-CoV-2 Not Binding Directly to ACE2 but Interacting Electrostatically With Lipid Raft in Salivary Glands","authors":"Hironori Tsuchiya","doi":"10.1111/jop.70082","DOIUrl":"10.1111/jop.70082","url":null,"abstract":"","PeriodicalId":16588,"journal":{"name":"Journal of Oral Pathology & Medicine","volume":"55 2","pages":"173-174"},"PeriodicalIF":2.3,"publicationDate":"2025-11-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145438273","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Salivary and Serum Cytokine Levels in Oral Chronic Graft-Versus-Host Disease: A Longitudinal Study on the Role of IL-6, IL-1α, and IL-17","authors":"Francesco Ferella,&nbsp;Letizia Nitro,&nbsp;Fabiana Allevi,&nbsp;Federico Biglioli,&nbsp;Matilde Coccapani,&nbsp;Giovanni Felisati,&nbsp;Giorgia Pugliese,&nbsp;Giorgio Ghilardi,&nbsp;Linda Montavoci,&nbsp;Anna Caretti,&nbsp;Alberto Maria Saibene","doi":"10.1111/jop.70076","DOIUrl":"10.1111/jop.70076","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Chronic graft-versus-host disease (cGVHD) is a significant complication following hematopoietic cell transplantation, often manifesting with oral lesions that severely impact quality of life. Cytokines such as IL-6, IL-1α, and IL-17 are implicated in inflammation and may serve as biomarkers for disease activity and therapeutic response in cGVHD.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>A prospective monocentric study was conducted on 10 patients with oral cGVHD at San Paolo Hospital, Milan, between April 2023 and January 2024. Salivary and serum levels of IL-6, IL-1α, and IL-17 were measured pre- and post- topical clobetasol treatment for the oral mucosa using enzyme-linked immunoassay (ELISA). Patients underwent clinical assessments for oral pain and dryness using visual analog scales (VAS) and the Oral Mucositis Rating Scale (OMRS). Wilcoxon and Spearman correlation tests were used for statistical analysis.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Significant reductions in serum IL-17 (<i>p</i> = 0.006) and trends towards reduced salivary IL-6 (<i>p</i> = 0.066) were observed post-treatment. No significant changes were found in IL-1α levels. Clinical assessments showed significant improvement in pain and OMRS scores post-therapy (<i>p</i> &lt; 0.05). No correlation was found between cytokine levels and clinical symptoms.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>While IL-6 and IL-17 play roles in cGVHD, only IL-17 showed significant reduction after topical steroid therapy. This suggests IL-17's potential as a marker for inflammatory activity in cGVHD. Serum IL-17 may serve as a useful biomarker in oral cGVHD. Future studies with larger cohorts are needed to further validate these findings and explore the role of salivary cytokines in disease monitoring.</p>\u0000 </section>\u0000 </div>","PeriodicalId":16588,"journal":{"name":"Journal of Oral Pathology & Medicine","volume":"55 2","pages":"201-208"},"PeriodicalIF":2.3,"publicationDate":"2025-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145390587","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Simple and Efficient Method for Analyzing Murine Gingival Immune Cells Using Flow Cytometry: An Animal Study","authors":"Wenting Jiang,&nbsp;Xuekang Wang,&nbsp;Yiping Wei,&nbsp;Ping Lv,&nbsp;Ying Wang,&nbsp;Wenjie Hu","doi":"10.1111/jop.70074","DOIUrl":"10.1111/jop.70074","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Elucidating the immune cell network is of great significance for understanding the pathogenesis of periodontitis. Flow cytometry is the preferred tool for quantifying the composition of immune cells in the pathological process of inflammation. Perfusion prior to tissue harvesting is necessary for flow cytometric analysis of various tissues. However, whether perfusion is necessary for flow cytometric analysis of murine gingival tissues has never been reported.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Method</h3>\u0000 \u0000 <p>Forty-eight mice were randomly divided into four groups: control + non-perfused group, control + perfused group, periodontitis + non-perfused group, and periodontitis + perfused group. Ligation-induced experimental periodontitis was established in periodontitis mice. The circulating blood was perfused out in the perfused groups before harvesting tissue samples. Flow cytometry was used to analyze the composition of immune cells in murine palatal gingival tissues.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Experimental periodontitis induced alveolar bone resorption and inflammatory cell infiltration. Perfusion can successfully remove blood from the vessels of murine periodontal tissues. Flow cytometric analysis showed that there was no significant difference in the proportion of various immune cells in murine gingival tissues between the perfusion groups and the non-perfusion groups.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>The present study was the first to use flow cytometry to compare perfused mouse gingiva with non-perfused mouse gingiva and found no significant difference in immune cell composition between the two groups. In addition, this study provides a simple and efficient protocol for the collection and processing of murine gingival tissue, which could provide facilities for understanding the role and mechanism of the immune cell network in periodontal diseases.</p>\u0000 </section>\u0000 </div>","PeriodicalId":16588,"journal":{"name":"Journal of Oral Pathology & Medicine","volume":"55 2","pages":"191-200"},"PeriodicalIF":2.3,"publicationDate":"2025-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145390468","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Clarified Açaí Supplementation and Photobiomodulation on the Parotid Glands of Rats Submitted to Chemotherapy","authors":"Wallacy Watson Pereira Melo,&nbsp;Walessa Alana Bragança Aragão,&nbsp;Maria Karolina Martins Ferreira,&nbsp;Vinicius Ruan Neves dos Santos,&nbsp;Leonardo Oliveira Bittencourt,&nbsp;Paulo Fernando Santos Mendes,&nbsp;Deborah Ribeiro Frazão,&nbsp;José Mário Matos-Sousa,&nbsp;Hadassa Helez Neves Ferreira,&nbsp;José Messias Perdigão,&nbsp;Hannah Gil de Farias Morais,&nbsp;Herve Rogez,&nbsp;Roseana de Almeida Freitas,&nbsp;Manoela Domingues Martins,&nbsp;Rafael Rodrigues Lima,&nbsp;Renata Duarte de Souza-Rodrigues","doi":"10.1111/jop.70072","DOIUrl":"10.1111/jop.70072","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>The antineoplastic drugs used in anticancer treatment regimens can induce changes in normal tissues, including salivary glands. This study evaluated the effects of clarified açaí and photobiomodulation (PBM) on the oxidative biochemistry and microstructure of the parotid glands of rats with oral mucositis.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Male rats (<i>n</i> = 54) were divided into five groups: Negative control (no mucositis); Positive control (mucositis without treatment); PBM; Clarified açaí; and PBM + clarified açaí. Oral mucositis was induced by intraperitoneal injection of 5-fluorouracil on days 0 (60 mg/kg) and 2 (40 mg/kg). On days 3 and 4, bilateral scarification of the buccal mucosa was performed. On days 0 (negative controls), 8 and 10 (other groups), parotid glands were collected. To evaluate oxidative biochemistry, the following analyses were performed: Antioxidant capacity test against peroxyl radicals (ACAP), Lipid Peroxidation Assay (LPO), and Nitric oxide metabolite (NOx). Additionally, histopathological, histomorphometric, and histochemical analyses were performed. Statistical analysis was performed using two-way ANOVA and Tukey's post-test (<i>p</i> &lt; 0.05).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Clarified açaí, alone or associated with photobiomodulation, increased antioxidant levels compared to the positive control on days 8 and 10. Regarding lipid peroxidation and nitric oxide metabolite, the positive control showed higher levels than the other groups. The morphological assessment showed that the clarified açaí and photobiomodulation groups maintained similar structures of the parenchyma, stroma, and acini to the negative control.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>The study results demonstrated that clarified açaí and photobiomodulation conferred biochemical and structural protection to the parotid glands against chemotherapy–induced damage.</p>\u0000 </section>\u0000 </div>","PeriodicalId":16588,"journal":{"name":"Journal of Oral Pathology & Medicine","volume":"55 1","pages":"120-132"},"PeriodicalIF":2.3,"publicationDate":"2025-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jop.70072","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145355065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Early Intervention of Subchondral Bone Resorption Mitigates Cartilage Degeneration in TMJOA","authors":"Meng-Nan Cao,&nbsp;Shi-Yang Feng,&nbsp;Chen-Chen Gao,&nbsp;Yang Xiao,&nbsp;Yi-Xin Li,&nbsp;Jie Lei,&nbsp;Kai-Yuan Fu","doi":"10.1111/jop.70073","DOIUrl":"10.1111/jop.70073","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Excessive subchondral bone resorption is a typical manifestation in the early stage of osteoarthritis. This study is to verify whether and when intervening in subchondral bone could alleviate cartilage degeneration of temporomandibular joint osteoarthritis.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Disc displacement without reduction was used to induce temporomandibular joint osteoarthritis. Alendronate was administered subcutaneously twice a week at a dosage of 60 μg/kg body weight for 1 week by two intervention methods: early administration (1 day after disc displacement without reduction surgery) and late administration (2 weeks after disc displacement without reduction surgery). Micro-CT was used to assess subchondral bone mass and microstructure. Hematoxylin–eosin staining, toluidine blue staining, tartrate-resistant acid phosphatase staining, immunofluorescence staining, and Western blot were applied to evaluate histopathological changes of the condylar cartilage and subchondral bone.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Early alendronate administration not only prevented subchondral bone resorption in early-stage temporomandibular joint osteoarthritis, but also suppressed chondrocyte apoptosis, cartilage extracellular matrix degeneration, as well as excessive subchondral bone formation of condyle in late-stage temporomandibular joint osteoarthritis. However, late alendronate administration had little effect on either subchondral bone or cartilage degenerative changes.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>Early inhibition of subchondral bone resorption could mitigate abnormal subchondral bone formation and condylar cartilage degeneration in late-stage temporomandibular joint osteoarthritis, which might be a promising strategy for temporomandibular joint osteoarthritis treatment.</p>\u0000 </section>\u0000 </div>","PeriodicalId":16588,"journal":{"name":"Journal of Oral Pathology & Medicine","volume":"55 1","pages":"133-146"},"PeriodicalIF":2.3,"publicationDate":"2025-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145345679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Panax notoginseng Saponins Alleviate OSF by Inhibiting Ferroptosis Through GPX4 Activation","authors":"Xinyue Zhang,&nbsp;Yujie Sun,&nbsp;Chenxi Zhao,&nbsp;Hong Zou,&nbsp;Liang Hu,&nbsp;Lixiang Wen,&nbsp;Qun Tang","doi":"10.1111/jop.70069","DOIUrl":"10.1111/jop.70069","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>Oral submucous fibrosis (OSF) is a chronic progressive disease characterized by fibrosis. <i>Panax notoginseng</i> saponins (PNS) are effective components of the traditional Chinese medicine <i>Panax notoginseng</i> and play an important role in the treatment of OSF. However, the underlying mechanism of action for this medicine remains unclear. The aim of this study was to explore the effects of ferroptosis in OSF.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Subjects and Methods</h3>\u0000 \u0000 <p>The mice were divided into six groups. Mouth openings were detected. The buccal tissues were harvested for histomorphological analysis, western blot, and RT-qPCR. The cells were harvested for CCK8 detection, western blot, RT-qPCR, TEM, immunofluorescence, fluorescent probe, and colorimetry.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>PNS improved mouth opening, alleviated tissue cell damage, and inhibited ferroptosis and fibrosis in OSF rat models. Our study found that ferroptosis is closely related to arecoline-induced OSF and that PNS inhibits ferroptosis by upregulating glutathione peroxidase 4 (GPX4) to alleviate OSF.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>The research established OSF in vivo and in vitro, respectively, and the changes in the expression of fibrosis and ferroptosis-related markers in these models were studied to provide a theoretical and experimental basis for the clinical prevention and treatment of OSF.</p>\u0000 </section>\u0000 </div>","PeriodicalId":16588,"journal":{"name":"Journal of Oral Pathology & Medicine","volume":"55 1","pages":"107-119"},"PeriodicalIF":2.3,"publicationDate":"2025-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145251591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}