Pub Date : 2017-04-25DOI: 10.4172/2150-3508.1000196
Rahmi Can Ozdemir, E.Ö. Aygül
The present study aimed to obtain gynogenetic zebrafish. For this purpose, zebrafish spermatozoa exposed to UV irradiation to make it haploid gynogenetic fish and heat shock was applied to haploid zygote in order to obtain gynogenetic diploid fish. Temperature, which is an important factor of the production, is taken into consideration in this study. In this respect, this study compared the results of 41.4°C and 41°C heat-shock applications and found that 12nd-24th-48th-72nd hour survival rate was maximum at 41,4°C (P<0.05). When considered from hatching rate (72th-78th hour) view at 41.4°C was 17.3 ± 3% in gynogenetic diploid group and heat-shock application at 41°C survival rate was 14 ± 2% in gynogenetic diploid group and there is no survivor in haploid group, was observed (P<0.05). The result of the karyotype analysis in haploid gynogenetic embryos, ruptured chromosome fragments was identified. Also in karyotype analysis of diploid gynogenetic embryos, 2n=50 chromosomes was identified. On 3rd day after fertilization, total body length of the haploid gynogenetic fish was 39.6% shorter and body thickness was 33% thicker than the diploid gynogenetic group. Our gynogenetic fish producing method and the different heat-shock applications improved survival rate of gynogenetic fish.
{"title":"Different Heat Shock Application Effect on Gynogenetic Production of Zebrafish (Danio Rerio)","authors":"Rahmi Can Ozdemir, E.Ö. Aygül","doi":"10.4172/2150-3508.1000196","DOIUrl":"https://doi.org/10.4172/2150-3508.1000196","url":null,"abstract":"The present study aimed to obtain gynogenetic zebrafish. For this purpose, zebrafish spermatozoa exposed to UV irradiation to make it haploid gynogenetic fish and heat shock was applied to haploid zygote in order to obtain gynogenetic diploid fish. \u0000Temperature, which is an important factor of the production, is taken into consideration in this study. In this respect, this study compared the results of 41.4°C and 41°C heat-shock applications and found that 12nd-24th-48th-72nd hour survival rate was maximum at 41,4°C (P<0.05). When considered from hatching rate (72th-78th hour) view at 41.4°C was 17.3 ± 3% in gynogenetic diploid group and heat-shock application at 41°C survival rate was 14 ± 2% in gynogenetic diploid group and there is no survivor in haploid group, was observed (P<0.05). \u0000The result of the karyotype analysis in haploid gynogenetic embryos, ruptured chromosome fragments was identified. Also in karyotype analysis of diploid gynogenetic embryos, 2n=50 chromosomes was identified. On 3rd day after fertilization, total body length of the haploid gynogenetic fish was 39.6% shorter and body thickness was 33% thicker than the diploid gynogenetic group. Our gynogenetic fish producing method and the different heat-shock applications improved survival rate of gynogenetic fish.","PeriodicalId":166175,"journal":{"name":"Fisheries and Aquaculture Journal","volume":"176 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116130539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-04-18DOI: 10.4172/2150-3508.1000195
E. Chow, K. H. Liong, E. Schoeters
A 12-weeks feeding trial was conducted to evaluate the effects of encapsulated butyric acid (ButiPEARL™) and microemulsified yellow carotenoid (Quantum GLO™ Y) on growth, immune parameters and their synergistic effect on pigmentation of hybrid catfish. In the experiment, the catfish was randomly divided into 12 groups of 15 fishes and then fed with four experimental diets containing 0.5 kg/t ButiPEARL™, 0.7 kg/t Quantum GLO™ Y, 0.5 kg/t ButiPEARL™ + 0.7 kg/t Quantum GLO™ Y, or none of these supplements (control diet). The results showed that the ButiPEARL™ + Quantum GLO™ Y fed group gave the highest yellowness (b*) score of 18.43 in the back muscle and almost double the total carotenoid measured in the fish muscle (151.35 mg/kg) compared to the Quantum™ Y fed group. This suggests that butyric acid in the diet had a synergistic effect on carotenoid absorption and pigmentation performance of catfish. The body weight of all treatment groups was significantly different from the control group and the catfish fed ButiPEARL™ alone had the highest body weight gained followed by the ButiPEARL™ + Quantum GLO™ Y fed group with an FCR improvement of 25 points and 13 points respectively over the control. There was no adverse effect on the immune system after feeding both butyric acid and carotenoid to the catfish and the immune parameters (number of leucocytes, erythrocyte, percent of hematocrit, haemoglobin and total protein) of ButiPEARL™ + Quantum GLO™ Y fed group were improved compared with the other groups. In conclusion, ButiPEARL™, Quantum GLO™ Y and the combination have positive effects on performance and pigmentation in catfish aquaculture.
{"title":"Dietary Encapsulated Butyric Acid (Butipearl™) and Microemulsified Carotenoids (Quantum GLO™ Y) on the Growth, Immune Parameters and their Synergistic Effect on Pigmentation of Hybrid Catfish ( Clarias macrocephalus × Clarias gariepinus )","authors":"E. Chow, K. H. Liong, E. Schoeters","doi":"10.4172/2150-3508.1000195","DOIUrl":"https://doi.org/10.4172/2150-3508.1000195","url":null,"abstract":"A 12-weeks feeding trial was conducted to evaluate the effects of encapsulated butyric acid (ButiPEARL™) and microemulsified yellow carotenoid (Quantum GLO™ Y) on growth, immune parameters and their synergistic effect on pigmentation of hybrid catfish. In the experiment, the catfish was randomly divided into 12 groups of 15 fishes and then fed with four experimental diets containing 0.5 kg/t ButiPEARL™, 0.7 kg/t Quantum GLO™ Y, 0.5 kg/t ButiPEARL™ + 0.7 kg/t Quantum GLO™ Y, or none of these supplements (control diet). The results showed that the ButiPEARL™ + Quantum GLO™ Y fed group gave the highest yellowness (b*) score of 18.43 in the back muscle and almost double the total carotenoid measured in the fish muscle (151.35 mg/kg) compared to the Quantum™ Y fed group. This suggests that butyric acid in the diet had a synergistic effect on carotenoid absorption and pigmentation performance of catfish. The body weight of all treatment groups was significantly different from the control group and the catfish fed ButiPEARL™ alone had the highest body weight gained followed by the ButiPEARL™ + Quantum GLO™ Y fed group with an FCR improvement of 25 points and 13 points respectively over the control. There was no adverse effect on the immune system after feeding both butyric acid and carotenoid to the catfish and the immune parameters (number of leucocytes, erythrocyte, percent of hematocrit, haemoglobin and total protein) of ButiPEARL™ + Quantum GLO™ Y fed group were improved compared with the other groups. In conclusion, ButiPEARL™, Quantum GLO™ Y and the combination have positive effects on performance and pigmentation in catfish aquaculture.","PeriodicalId":166175,"journal":{"name":"Fisheries and Aquaculture Journal","volume":"57 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115730620","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-04-08DOI: 10.4172/2150-3508.1000194
Adamu Yimer, M. Mingist, B. Bekele
The objective of this study was to evaluate the quality of fish processed by open air rack, solar tent and smoking methods. African catfish (Clarias gariepinus) was filleted, washed, sliced, brine salted and processed by the selected methods, packed in plastic bags and stored at room temperature. Abalo (Brucea antidysenterica) and Olic tree (Olea europaea) were used as smoking wood. For moisture content test, 25 g of processed fish was put in an oven at 105°C and weight change of the samples was measured until the change become constant. It was calculated as the difference between the initial and final weight. Twenty-five gram of processed fish was taken aseptically and standard procedures of dilution and spread plating were done based on the type of microorganism to be identified. Then, the number of colonies were counted and changed into log 10 cfu/g. Solar tent reduced the moisture content to 20% and 23% for Nile tilapia and African catfish, respectively. Microbial load of solar tent dried fish samples was below the standard norm than open air rack and smoking methods. There was statistical difference between treatments (p=0.05). Solar tent drier produced better quality of fish product.
{"title":"Microbial Evaluation of Selected Post Harvest Processing Techniques for Quality Fish Product at Bahir Dar Town, Ethiopia","authors":"Adamu Yimer, M. Mingist, B. Bekele","doi":"10.4172/2150-3508.1000194","DOIUrl":"https://doi.org/10.4172/2150-3508.1000194","url":null,"abstract":"The objective of this study was to evaluate the quality of fish processed by open air rack, solar tent and smoking methods. African catfish (Clarias gariepinus) was filleted, washed, sliced, brine salted and processed by the selected methods, packed in plastic bags and stored at room temperature. Abalo (Brucea antidysenterica) and Olic tree (Olea europaea) were used as smoking wood. For moisture content test, 25 g of processed fish was put in an oven at 105°C and weight change of the samples was measured until the change become constant. It was calculated as the difference between the initial and final weight. Twenty-five gram of processed fish was taken aseptically and standard procedures of dilution and spread plating were done based on the type of microorganism to be identified. Then, the number of colonies were counted and changed into log 10 cfu/g. Solar tent reduced the moisture content to 20% and 23% for Nile tilapia and African catfish, respectively. Microbial load of solar tent dried fish samples was below the standard norm than open air rack and smoking methods. There was statistical difference between treatments (p=0.05). Solar tent drier produced better quality of fish product.","PeriodicalId":166175,"journal":{"name":"Fisheries and Aquaculture Journal","volume":"70 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129104810","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-03-23DOI: 10.4172/2150-3508.1000192
M. Harlıoğlu, Ardavan Farhadi
Iran’s appropriate geographical location (i.e., large brackish water source in the north, Caspian Sea, salt water source in the south, Persian Gulf and Gulf of Oman) and a wide range of brackish, freshwater and marine species (i.e., trout, carp, sturgeon, sea bass, sea bream, turbot, mackerel, sardine, tuna, sea cucumber, marine shrimp, crayfish) provides Iran to be a great fish producer country. The total fishery production was 947,352 tons in 2014. In this year, 575,512 tons (60.74% of total fishery production) of fish production were obtained from the capture fisheries and 371,840 tons (39.26% of total fishery production) of production was obtained from the aquaculture production. Fisheries in the Persian Gulf and Gulf of Oman are the most important fishery (93% of total fishery) in Iran. There has been a fast increase in the aquaculture production in Iran. For example, total aquaculture production for 2004 and 2014 was 124,560 and 349,365 tons, respectively. Therefore, the percentage of aquaculture in total fish production has been rising every year. The ratio of aquaculture production to total fish production was 26.26% in 2004, 32.65% in 2008 and 39.26% in 2014. Rainbow trout and carps are the main cultured freshwater fish species. In recent years, fisheries production export of Iran has been increased from US$ 85 million in 2004 to US$ 300 million in 2014. In conclusion, despite Iran’s long coastline, fishery has not been developed completely and has the potential to be developed more by enhancing aquaculture and fish cage culture.
{"title":"Iranian Fisheries Status: An Update (2004-2014)","authors":"M. Harlıoğlu, Ardavan Farhadi","doi":"10.4172/2150-3508.1000192","DOIUrl":"https://doi.org/10.4172/2150-3508.1000192","url":null,"abstract":"Iran’s appropriate geographical location (i.e., large brackish water source in the north, Caspian Sea, salt water source in the south, Persian Gulf and Gulf of Oman) and a wide range of brackish, freshwater and marine species (i.e., trout, carp, sturgeon, sea bass, sea bream, turbot, mackerel, sardine, tuna, sea cucumber, marine shrimp, crayfish) provides Iran to be a great fish producer country. The total fishery production was 947,352 tons in 2014. In this year, 575,512 tons (60.74% of total fishery production) of fish production were obtained from the capture fisheries and 371,840 tons (39.26% of total fishery production) of production was obtained from the aquaculture production. Fisheries in the Persian Gulf and Gulf of Oman are the most important fishery (93% of total fishery) in Iran. There has been a fast increase in the aquaculture production in Iran. For example, total aquaculture production for 2004 and 2014 was 124,560 and 349,365 tons, respectively. Therefore, the percentage of aquaculture in total fish production has been rising every year. The ratio of aquaculture production to total fish production was 26.26% in 2004, 32.65% in 2008 and 39.26% in 2014. Rainbow trout and carps are the main cultured freshwater fish species. In recent years, fisheries production export of Iran has been increased from US$ 85 million in 2004 to US$ 300 million in 2014. In conclusion, despite Iran’s long coastline, fishery has not been developed completely and has the potential to be developed more by enhancing aquaculture and fish cage culture.","PeriodicalId":166175,"journal":{"name":"Fisheries and Aquaculture Journal","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116906907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-03-23DOI: 10.4172/2150-3508.1000193
B. C. Patra, Shampa Patra, M. Bhattacharya
Early fingerlings of Anabas testudineus were nurtured in the laboratory for six weeks under altered nutritional status (different dietary protein level and source) and feeding regimes (ad libitum, ‘limited’, and ‘starvation’ intervals). Their RNA/DNA, Ca/P ratios and protein biosynthesis were determined with highly sensitive fluorescence technique, spectrophotometer and liquid scintillation system respectively. Well-fed early fingerlings (4-6 weeks age group) having higher RNA/DNA (2.11), Ca/P (1.82) ratio and protein biosynthesis rates both in muscle and liver have significantly differs from fingerlings with ‘limited’ food supply and 'starvation' intervals. Rate of protein biosynthesis >244.33 & 603.24 in muscle & liver respectively (count per minute mg protein-1) when compared with well-fed fingerlings and reared under 'limited' food supply and 'starvation' intervals. At ‘starvation’ interval no significant differences were recorded due to different diet treatment. However, fingerlings showed higher mortalities, decreased growth, RNA/DNA, Ca/P ratio and protein biosynthesis rate that may be caused by malnutrition. Fingerlings (early) sampled after 42 days in nearby areas during late monsoon (September) showed RNA/DNA, Ca/P ratio and protein biosynthesis rate were surprisingly low almost as low as ‘starved’ fingerlings, when judged on the basis of mean body weight as compared to well fed fish. It was also observed under microscope that some of these fingerlings were seriously affected by an intestinal disease and this may be due to the ingestion of unsuitable copepods. The low RNA/DNA, Ca/P ratio and protein biosynthesis rate in field samples and ‘starved’ fish are, therefore, interpreted as a sub-lethal result of this disease and malnutrition respectively.
{"title":"Evaluating the Nutritional Condition of an Indian Climbing Perch, Anabas Testudineus Fingerlings by the RNA/DNA, Ca/P Ratio and Protein Bio-Synthesis in Liver and Muscle","authors":"B. C. Patra, Shampa Patra, M. Bhattacharya","doi":"10.4172/2150-3508.1000193","DOIUrl":"https://doi.org/10.4172/2150-3508.1000193","url":null,"abstract":"Early fingerlings of Anabas testudineus were nurtured in the laboratory for six weeks under altered nutritional status (different dietary protein level and source) and feeding regimes (ad libitum, ‘limited’, and ‘starvation’ intervals). Their RNA/DNA, Ca/P ratios and protein biosynthesis were determined with highly sensitive fluorescence technique, spectrophotometer and liquid scintillation system respectively. Well-fed early fingerlings (4-6 weeks age group) having higher RNA/DNA (2.11), Ca/P (1.82) ratio and protein biosynthesis rates both in muscle and liver have significantly differs from fingerlings with ‘limited’ food supply and 'starvation' intervals. Rate of protein biosynthesis >244.33 & 603.24 in muscle & liver respectively (count per minute mg protein-1) when compared with well-fed fingerlings and reared under 'limited' food supply and 'starvation' intervals. At ‘starvation’ interval no significant differences were recorded due to different diet treatment. However, fingerlings showed higher mortalities, decreased growth, RNA/DNA, Ca/P ratio and protein biosynthesis rate that may be caused by malnutrition. Fingerlings (early) sampled after 42 days in nearby areas during late monsoon (September) showed RNA/DNA, Ca/P ratio and protein biosynthesis rate were surprisingly low almost as low as ‘starved’ fingerlings, when judged on the basis of mean body weight as compared to well fed fish. It was also observed under microscope that some of these fingerlings were seriously affected by an intestinal disease and this may be due to the ingestion of unsuitable copepods. The low RNA/DNA, Ca/P ratio and protein biosynthesis rate in field samples and ‘starved’ fish are, therefore, interpreted as a sub-lethal result of this disease and malnutrition respectively.","PeriodicalId":166175,"journal":{"name":"Fisheries and Aquaculture Journal","volume":"6 3 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123688944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-03-03DOI: 10.4172/2150-3508.1000189
Wehye As, Amponsah Skk, J. As
This study examined some aspects of population dynamics of 1776 specimen of S. maderensis (Lowe, 1988) from Liberian coastal waters, from April 2013 to September 2013 (total of six months) using the FiSAT II for analysis. From the results, the growth was assumed to follow the von Bertalanffy growth function with asymptotic length (L∞) and the growth coefficient (K) estimated at 44.63 cm total length and 0.38 year-1 respectively. The growth performance index, longevity and the theoretical age at birth (t0) were estimated as 2.88, 7.51 years and -0.387 year-1 respectively. The length at first capture (Lc50=13.99 cm) was lower than the length at first maturity (Lm50=29.75 cm), an indication that most of the harvested stock were juveniles. Instantaneous rate of total mortality, natural mortality and fishing mortality were estimated as 1.24 year-1, 0.81 year-1 and 0.43 year-1 respectively. The current exploitation rate (E) and maximum exploitation rate (Emax) were calculated as 0.34 and 0.36 respectively. Results from the study indicated that the exploitation of S. maderensis is at the maximum sustainable yield coupled with the presence of growth overfishing and intense fishing pressure. Therefore, urgent management actions including increasing fishing gears mesh size and regulating fishing effort is needed to protect the S. maderensis stock.
{"title":"Growth, Mortality and Exploitation of Sardinella maderensis (Lowe, 1838) in the Liberian coastal waters","authors":"Wehye As, Amponsah Skk, J. As","doi":"10.4172/2150-3508.1000189","DOIUrl":"https://doi.org/10.4172/2150-3508.1000189","url":null,"abstract":"This study examined some aspects of population dynamics of 1776 specimen of S. maderensis (Lowe, 1988) from Liberian coastal waters, from April 2013 to September 2013 (total of six months) using the FiSAT II for analysis. From the results, the growth was assumed to follow the von Bertalanffy growth function with asymptotic length (L∞) and the growth coefficient (K) estimated at 44.63 cm total length and 0.38 year-1 respectively. The growth performance index, longevity and the theoretical age at birth (t0) were estimated as 2.88, 7.51 years and -0.387 year-1 respectively. The length at first capture (Lc50=13.99 cm) was lower than the length at first maturity (Lm50=29.75 cm), an indication that most of the harvested stock were juveniles. Instantaneous rate of total mortality, natural mortality and fishing mortality were estimated as 1.24 year-1, 0.81 year-1 and 0.43 year-1 respectively. The current exploitation rate (E) and maximum exploitation rate (Emax) were calculated as 0.34 and 0.36 respectively. Results from the study indicated that the exploitation of S. maderensis is at the maximum sustainable yield coupled with the presence of growth overfishing and intense fishing pressure. Therefore, urgent management actions including increasing fishing gears mesh size and regulating fishing effort is needed to protect the S. maderensis stock.","PeriodicalId":166175,"journal":{"name":"Fisheries and Aquaculture Journal","volume":"49 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122454287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-03-03DOI: 10.4172/2150-3508.1000190
Yajuan Li, Da‐long Ren, Min Chen, Shuchao Ge, Bing Hu
Uncontrolled migration and excess recruitment of neutrophils can lead to persistent inflammation, tissue damage and disease. Myeloperoxidase is a remarkable target for further understanding the immune cell migration. This study tests the hypothesis that myeloperoxidase may regulate the immune cell activity and provides a new perspective on the treatment of immune diseases by exploring the mechanism of neutrophil migration. Studies of leukocyte migration in vivo in the zebrafish model, which set a collection of advantages both mammalian and cell lines, have gained widespread attention. In this study, we used tg (coro1a: eGFP; lyz: dsred2) and tg (lyz: eGFP) lines labelling both macrophages and neutrophils to study the effect of mpx on neutrophil chemotaxis to wounds. We found that myeloperoxidase was required for neutrophil migration to the wound site in injury-induced inflammation, but not required for neutrophil migration to the infection site in the infection-induced inflammation. Further, the regulation of myeloperoxidase was specific to neutrophil migration to wound inflammation, but was not necessary for macrophage migration. Thus, myeloperoxidase activity shows therapeutic potential for inflammatory disease related to neutrophil migration.
{"title":"Myeloperoxidase Inactivation Affects Neutrophil Recruitment in Zebrafish Injury-Induced Model","authors":"Yajuan Li, Da‐long Ren, Min Chen, Shuchao Ge, Bing Hu","doi":"10.4172/2150-3508.1000190","DOIUrl":"https://doi.org/10.4172/2150-3508.1000190","url":null,"abstract":"Uncontrolled migration and excess recruitment of neutrophils can lead to persistent inflammation, tissue damage and disease. Myeloperoxidase is a remarkable target for further understanding the immune cell migration. This study tests the hypothesis that myeloperoxidase may regulate the immune cell activity and provides a new perspective on the treatment of immune diseases by exploring the mechanism of neutrophil migration. Studies of leukocyte migration in vivo in the zebrafish model, which set a collection of advantages both mammalian and cell lines, have gained widespread attention. In this study, we used tg (coro1a: eGFP; lyz: dsred2) and tg (lyz: eGFP) lines labelling both macrophages and neutrophils to study the effect of mpx on neutrophil chemotaxis to wounds. We found that myeloperoxidase was required for neutrophil migration to the wound site in injury-induced inflammation, but not required for neutrophil migration to the infection site in the infection-induced inflammation. Further, the regulation of myeloperoxidase was specific to neutrophil migration to wound inflammation, but was not necessary for macrophage migration. Thus, myeloperoxidase activity shows therapeutic potential for inflammatory disease related to neutrophil migration.","PeriodicalId":166175,"journal":{"name":"Fisheries and Aquaculture Journal","volume":"39 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130422204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-01-18DOI: 10.4172/2150-3508.1000188
Ayeloja Aa, F. George, E. Sodeeq, Adebisi Gl
The study investigated the effect of price modulation policy of Federal Government of Nigeria on fish production in Oyo State, South West Nigeria. A multistage sampling technique was used to select 150 respondents from 9 wards within Oyo State using well-structured questionnaire to obtain information on socio-economic characteristics of the respondents, types of fish cultured and the impact of price modulation policy on profitability of fish production. Data were analyzed using descriptive statistics, regression and t-test analysis. There is influx of young men (57.5%) in fish production in the study area with 95% of them having between 1-5 years of experience. Clarias spp is the most cultured fish species in the study area and cost per kg of fish produced after price modulation had a negative and significant (p<0.05) effect on profitability of fish production during the period of this study. T-test further showed that there was significant difference between production cost before price modulation (PCBF) and production cost after price modulation (PCAF). This could have negative health and welfare implications on the citizenry. Policy makers should therefore put in place welfare packages that will ameliorate the effect of this policy.
{"title":"Price Modulation Policy of Federal Government of Nigeria: Effects on Fish Production","authors":"Ayeloja Aa, F. George, E. Sodeeq, Adebisi Gl","doi":"10.4172/2150-3508.1000188","DOIUrl":"https://doi.org/10.4172/2150-3508.1000188","url":null,"abstract":"The study investigated the effect of price modulation policy of Federal Government of Nigeria on fish production \u0000 in Oyo State, South West Nigeria. A multistage sampling technique was used to select 150 respondents from 9 \u0000 wards within Oyo State using well-structured questionnaire to obtain information on socio-economic characteristics \u0000 of the respondents, types of fish cultured and the impact of price modulation policy on profitability of fish production. \u0000 Data were analyzed using descriptive statistics, regression and t-test analysis. There is influx of young men (57.5%) \u0000 in fish production in the study area with 95% of them having between 1-5 years of experience. Clarias spp is the \u0000 most cultured fish species in the study area and cost per kg of fish produced after price modulation had a negative \u0000 and significant (p<0.05) effect on profitability of fish production during the period of this study. T-test further showed \u0000 that there was significant difference between production cost before price modulation (PCBF) and production cost \u0000 after price modulation (PCAF). This could have negative health and welfare implications on the citizenry. Policy \u0000 makers should therefore put in place welfare packages that will ameliorate the effect of this policy.","PeriodicalId":166175,"journal":{"name":"Fisheries and Aquaculture Journal","volume":"58 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129219155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2016-12-10DOI: 10.4172/2150-3508.1000179
M. Raja, R. Raja, P. Perumal
The microhabitat preference of zebrafish, Danio rerio from Karala river was investigated. The availability/ preference of microhabitat variables such as (i) distance from the stream bank (ii) water column depth (iii) mesohabitat (iv) water velocity (v) substratum and (vi) subaquatic vegetal cover were quantified based on underwater observations covering a total of 400 m2 of stream area. Microhabitat selectivity was analyzed by comparing the microhabitat availability in the study site and the microhabitat used by fish. Data availability and microhabitats usage pattern were used for calculating habitat availability/preference of D. rerio. In order to test the microhabitat preference of D. rerio the Principal Component Analysis (PCA) and Canonical Correspondence Analysis (CCA) were applied. In PCA the first three components with higher Eigenvalue accounted for about 98.46% of the total variance. The first component axis alone explained 86.33% of the variance with an Eigenvalue of 5.3 with high loadings (>0.7). CCA revealed a clear separation of the D. rerio along the microhabitat variables. Among the thirty two microhabitat variables, the forward selection procedure was found to be positively significant with eighteen microhabitat variables P<0.05. The preference of D. rerio in the studied streams viz: pool, plunge pool and isolated pool; slow flow, moderate flow and fast flow; and the substratum parameters: sand, gravel and leaf litter; depth parameters like Depth 1, Depth 2, Depth 3 and Depth 5; fish cover parameters such as root undercut and distance from bank parameters such as Distance from bank 1, Distance from bank 2 and Distance from bank 3. CCA revealed a clear distribution pattern of the D. rerio along the microhabitat variables.
{"title":"Zebrafish in the Wild: Microhabitat Use by Zebrafish Danio rerio (Hamilton, 1822) from Karala River of Jalpaiguri District, Northern Bengal, India","authors":"M. Raja, R. Raja, P. Perumal","doi":"10.4172/2150-3508.1000179","DOIUrl":"https://doi.org/10.4172/2150-3508.1000179","url":null,"abstract":"The microhabitat preference of zebrafish, Danio rerio from Karala river was investigated. The availability/ preference of microhabitat variables such as (i) distance from the stream bank (ii) water column depth (iii) mesohabitat (iv) water velocity (v) substratum and (vi) subaquatic vegetal cover were quantified based on underwater observations covering a total of 400 m2 of stream area. Microhabitat selectivity was analyzed by comparing the microhabitat availability in the study site and the microhabitat used by fish. Data availability and microhabitats usage pattern were used for calculating habitat availability/preference of D. rerio. In order to test the microhabitat preference of D. rerio the Principal Component Analysis (PCA) and Canonical Correspondence Analysis (CCA) were applied. In PCA the first three components with higher Eigenvalue accounted for about 98.46% of the total variance. The first component axis alone explained 86.33% of the variance with an Eigenvalue of 5.3 with high loadings (>0.7). CCA revealed a clear separation of the D. rerio along the microhabitat variables. Among the thirty two microhabitat variables, the forward selection procedure was found to be positively significant with eighteen microhabitat variables P<0.05. The preference of D. rerio in the studied streams viz: pool, plunge pool and isolated pool; slow flow, moderate flow and fast flow; and the substratum parameters: sand, gravel and leaf litter; depth parameters like Depth 1, Depth 2, Depth 3 and Depth 5; fish cover parameters such as root undercut and distance from bank parameters such as Distance from bank 1, Distance from bank 2 and Distance from bank 3. CCA revealed a clear distribution pattern of the D. rerio along the microhabitat variables.","PeriodicalId":166175,"journal":{"name":"Fisheries and Aquaculture Journal","volume":"8 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131713567","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2016-12-05DOI: 10.4172/2150-3508.1000185
W. O. Oyediran, A. Omoare, Oladoyinbo Ob, B. O. Ajagbe, T. T. Dick
Constraints limiting the effective utilization of low-cost fish processing technologies among women in selected fishing communities of Lagos State, Nigeria were examined in this study. Ninety-six fish processors were purposively selected as sample size for this study. Chi-square analysis was used for the hypothesis testing in this study to examine the association between constraints and utilization. The mean age of the respondents was 37.8 years, 49.0% had primary school education and 77.1% got skills from their parents. Majority of the respondents did not utilize galvanized smoking kiln (92.7%) and smoking chokor (69.8%). High cost of processing equipment (2.75), poor electricity supply (2.74), poor awareness creation and capacity building (2.72) and inadequate extension support (2.67) as the major problems limiting the utilization of low-cost fish processing technologies in the study area. Result of chi-square presented reveals that significant relationship existed between constraints and utilization of low-cost fish processing technologies at p<0.05 level of significance. It is therefore recommended that effort should be geared towards awareness creation and public enlightenment to promote adoption of low-cost fish processing technologies in the study areas.
{"title":"Constraints Limiting the Effective Utilization of Low-Cost Fish Processing Technologies among Women in Selected Fishing Communities of Lagos State, Nigeria","authors":"W. O. Oyediran, A. Omoare, Oladoyinbo Ob, B. O. Ajagbe, T. T. Dick","doi":"10.4172/2150-3508.1000185","DOIUrl":"https://doi.org/10.4172/2150-3508.1000185","url":null,"abstract":"Constraints limiting the effective utilization of low-cost fish processing technologies among women in selected fishing communities of Lagos State, Nigeria were examined in this study. Ninety-six fish processors were purposively selected as sample size for this study. Chi-square analysis was used for the hypothesis testing in this study to examine the association between constraints and utilization. The mean age of the respondents was 37.8 years, 49.0% had primary school education and 77.1% got skills from their parents. Majority of the respondents did not utilize galvanized smoking kiln (92.7%) and smoking chokor (69.8%). High cost of processing equipment (2.75), poor electricity supply (2.74), poor awareness creation and capacity building (2.72) and inadequate extension support (2.67) as the major problems limiting the utilization of low-cost fish processing technologies in the study area. Result of chi-square presented reveals that significant relationship existed between constraints and utilization of low-cost fish processing technologies at p<0.05 level of significance. It is therefore recommended that effort should be geared towards awareness creation and public enlightenment to promote adoption of low-cost fish processing technologies in the study areas.","PeriodicalId":166175,"journal":{"name":"Fisheries and Aquaculture Journal","volume":"73 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128213885","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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