Journal of oral science最新文献

Purpose: Inhibitory synaptic currents from fast-spiking neurons (FSNs), a typical gamma-aminobutyric acid (GABA)ergic interneuron in the cerebral cortex, to pyramidal neurons are facilitated by insulin. FSNs frequently show electrical synapses to FSNs, however, the effect of insulin on these electrical synapses is unknown. The aim of this study was to evaluate effects of insulin on electrical synaptic potentials between FSNs.

Methods: Electrical synaptic potentials via gap junctions between FSNs were recorded to examine how insulin modulates these potentials in the rat insular cortex (IC).

Results: Bath application of insulin (10 nM), which increases the spike firing rate of pyramidal neurons and unitary inhibitory postsynaptic currents recorded from FSN to pyramidal neuron connections, slightly but significantly increased electrical synaptic currents. The mean ratio of electrical synapses, the coupling coefficient that is obtained by postsynaptic voltage responses divided by presynaptic voltage amplitude, was 8.3 ± 1.1% in control and 9.2 ± 1.1% (n = 14) during 10 nM insulin application. Input resistance and voltage responses to large hyperpolarizing currents (-140 pA) were not changed by insulin.

Conclusion: These results suggest that insulin facilitates spike synchronization by increasing electrical synaptic currents via gap junctions of GABAergic FSNs in the IC.

Purpose: To compare the plaque-removal efficacy of ultra-soft single-headed, triple-headed, and T-shaped toothbrushes and the subjective perceptions of users.

Methods: In this randomized, single-blind, crossover study, 30 healthy adult participants were randomly divided into three groups of 10, who tested the three types of toothbrushes in differing order, with a 1-week washout period between the tests. Dental plaque scores were recorded before and after toothbrushing, and questionnaires on subjective perceptions after toothbrushing were collected.

Results: The participants included 16 female participants and 14 male participants (average age: 29.4 ± 6.7 years). All three toothbrush types were effective in reducing dental plaque by 36-46%; however, the cleaning effect of the ultra-soft single-headed toothbrush was inferior to that of the triple-headed and T-shaped toothbrushes, the difference being statistically significant. The users' subjective perceptions suggested that the ultra-soft single-headed toothbrush was significantly superior to the other two toothbrush types. Plaque scores for the lower posterior tooth surfaces were higher than those for the upper posterior tooth surfaces, particularly on the left side, for both the ultra-soft single-headed and T-shaped toothbrushes.

Conclusion: Healthcare practitioners should identify the type of toothbrush bristles used by individuals and provide appropriate oral health information.

Purpose: The aim of this investigation was to determine whether the facial esthetics of subjects rated as "attractive" can be related to specific cephalometric soft tissue parameters.

Methods: The profile silhouettes of 100 subjects (45 males and 55 females) were rated by 60 esthetics specialists (20 orthodontists, 20 dentists, and 20 plastic surgeons) using a Likert scale, and 30 of the subjects were selected as being "attractive". The cephalometric measurements of the attractive group were then compared with norms for the general Caucasian population.

Results: The effects of specific measured parameters on profile beauty grades were assessed using the median test, and the following variables were found to show significant correlations between the Caucasian norms and the attractive profile group: the ratio of the upper to lower face height (P = 0.011), the ratio of the subnasale - labrale inferius (Sn-Li) and labrale inferius - menton (Li-Me`) lines (P = 0.011), the distance between the chin and the subnasale perpendicular (P = 0.002), upper lip thickness (P = 0.021), soft tissue chin thickness (P = 0.021), vertical height ratio (P = 0.021), and nasolabial angle (P = 0.021).

Conclusion: A straight profile with a fuller and more protruded upper lip, a higher nasal tip, and a smaller lower facial third are considered to be the most attractive facial features, and may be useful for improvement of facial esthetics.

Purpose: To evaluate the effect of intra-radicular cleaning protocols after post-space preparation (PSP) on marginal adaptation of a luting agent to root dentin.

Methods: Eighty bovine incisors were sectioned transversely to obtain 18-mm-long root sections. The roots were prepared with ProTaper instruments and filled using Tagger's hybrid technique. PSP was performed at a length of 14 mm using #3-5 Largo drills. The roots were distributed into eight groups according to the chelating solution used (17% EDTA or 0.2% Chitosan) and the chelating activation/delivery method employed (conventional needle irrigation [CNI], EndoActivator [EA], XP-Endo Finisher [XPF] or passive ultrasonic irrigation [PUI]). The fiber posts were cemented using self-adhesive resin cement, and the roots were sectioned to obtain three discs from each root third. The dentin discs were observed under an optical microscope at ×40 magnification to assess marginal adaptation. The different protocols were compared considering the frequency of the marginal adaptation scores using the Kruskal-Wallis test, followed by the Dwass-Steel-Critchlow-Fligner test (α = 5%).

Results: The use of 17% EDTA resulted in better marginal adaptation (P < 0.001). PUI yielded results that were similar to those for EA (P = 0.949) and superior to those for XPF (P = 0.020). The combination of 17% EDTA + PUI provided a greater number of interfaces without marginal gaps (P < 0.001).

Conclusion: The cleaning protocol influenced the marginal adaptation of resin cement to root dentin.

Purpose: Osteoradionecrosis of the jaw is a therapy-resistant condition that may occur after treatment for head and neck cancer. The aim of this study was to investigate the incidence of osteoradionecrosis in patients with oropharyngeal cancer in relation to tooth extraction prior to radiation therapy.

Methods: Patients who had undergone radiation therapy for oropharyngeal cancer 5-10 years earlier were included and evaluated for the development of osteoradionecrosis (n = 75).

Results: Among the 75 patients, 62 had molar teeth present in the >50 Gy radiation field and 36 of those patients had teeth extracted prior to radiation therapy. Extraction of molars before radiotherapy significantly increased the risk of developing osteoradionecrosis (P < 0.05). There were no identifiable statistically significant correlations between the time from tooth extraction and the start of radiation therapy, the number of teeth in the radiation field, smoking habits, human papillomavirus-status, gender, age or tumor location and the development of osteoradionecrosis.

Conclusion: Tooth extraction prior to radiation therapy increases the risk of developing osteoradionecrosis. For patients with good oral hygiene and absence of dental disease, avoidance of tooth extraction in the radiation field could therefore reduce the risk of complications.

Purpose: The purpose of this study was to conduct basic research on the possibility of using cartilage tissue for hard-tissue reconstruction and to observe morphological changes in the transition of the cartilage to bone.

Methods: A 4-mm diameter bone defect was created in the right mandibular angle of rats. Cartilage, autologous bone, and artificial bone were grafted into the defect. Computed tomography (CT) was performed to measure the increase in bone volume. Further histological evaluation of the grafted site was performed.

Results: At 12 weeks, CT show that bone formation in the costal cartilage group was comparable to that in the autogenous bone group. Histologically, in the artificial bone group, a clear boundary was observed between the existing bone and defect, whereas in the costal cartilage and autologous bone groups, laminar plate bone repair of the defect was observed.

Conclusion: The findings in this study suggest that bone reconstruction achieved with cartilage grafting is almost equivalent to that with autogenous bone grafting and that bone reconstruction using cartilage is clinically feasible. In future, if regenerated cartilage is successfully applied clinically, bone reconstruction using regenerated cartilage may be feasible.

Purpose: To assess root temperature during filling techniques and quantify the volume of endodontic filling materials using infrared thermography (IT) and micro-computed tomography (micro-CT).

Methods: Ninety premolars were divided into three groups: lateral condensation (LC), single cone (SC) and thermomechanical compaction (TMC). For thermal analysis, 45 teeth were assessed using a FLIR T650sc IT camera during filling techniques and 45 teeth were scanned using a Nikon micro-CT to assess gutta-percha, cement, and void volumes. Descriptive and inferential statistical analyses were performed (non-parametric Mann-Whitney test, Kruskal-Wallis test, and Friedman test with Tukey's bidirectional analysis of variance).

Results: TMC showed the highest temperature increase at 15 s after the procedure and a significant temperature decrease at 45 s after its completion. TMC showed the largest volume of gutta-percha and LC the highest void volume.

Conclusion: The temperature increase generated by gutta-percha endodontic filling techniques is within acceptable limits. A greater volume of endodontic cement was observed for the SL and LC filling techniques.

Purpose: The purpose of this study was to assess the pull-out bond strength (PBS) of endodontically treated anterior teeth that had been restored with monolithic zirconia endocrowns using different extension depths (EDs) and post-and-core crowns after chewing simulation.

Methods: Thirty-six maxillary central teeth were used (n = 12). Group I: Glass fiber post-and-core crown, Group II: Endocrown with 3-mm EDs, Group III: Endocrown with 5-mm EDs. Restorations were fabricated from monolithic zirconia blocks using a CAM (computer-aided manufacturing) device. For cementation, conventional resin luting agent (Multilink N) was used. All samples were aged with a chewing simulator and PBS tests were conducted at a speed of 1 mm/min using an electromechanical servo universal testing machine. The values were recorded in MPa by dividing the failure load by the bonding area. One-way ANOVA and the post-hoc Tukey test were used for statistical analysis (P = 0.05).

Results: Group III demonstrated significantly greater PBS values than Groups I and II (P = 0.001). No significant difference was found between Groups I and II (P = 0.072).

Conclusion: Increasing the ED of an endocrown influences the PBS of endodontically treated anterior teeth restored with monolithic zirconia endocrowns.

This study aimed to clarify the usefulness of automatic cleaning in analysis of occlusal force. The maximum occlusal force was calculated in 151 healthy adults before cleaning (B), after manual cleaning (M), and after automatic cleaning (A) and compared among the three conditions. The absolute value of the error rate of A, based on M, was calculated. The occlusal forces in M and A were similar and significantly smaller than the occlusal force in B. The absolute error rate of A was 2.4%. These results suggest that automatic cleaning has a similar effect to manual cleaning and can be applied clinically.

Superficial angiomyxomas are myxoid mesenchymal tumors, and intraoral superficial angiomyxoma is extremely rare. This paper reports a novel case of a 41-year-old Japanese male patient with a 32 × 22 mm superficial angiomyxoma in the right soft palate. Tumor resection was performed and a polyglycolic acid sheet was attached. Over a 28-month follow-up, there was no evidence of disease recurrence. This paper also reviewed 11 cases of intraoral superficial angiomyxomas reported in previous literature. The condition was more common among middle-aged men. Surgical resection was the most common treatment, and local recurrence was observed in only one case.