Pub Date : 2009-03-06DOI: 10.2174/1874840600902010017
T. Seran, I. Brintha
A field experiment was conducted at the Agronomy farm, Eastern University, Sri Lanka to study the biological and economic efficiency of radish (Raphanus sativus L.) intercropped with vegetable amaranthus (Amaranthus tricolor L.). Treatments were radish as sole crop (T1), vegetable amaranthus as sole crop (T2), 20/50 cm paired row radish with three (T3) or four (T4) rows of vegetable amaranthus in between paired rows and 25/40 cm paired row radish with three (T5) or two (T6) rows of vegetable amaranthus in between paired rows. The results showed that, there was no significant difference (P>0.05) in area time equivalency ratio (ATER) among intercropping treatments and significant differences (P<0.05) were obtained in the land equivalent ratio (LER) and crop performance ratio (CPR). LER (1.31) and CPR (1.71) were superior in T3 and T4 respectively. In case of economic indices, gross return was significantly differed (P<0.01) among treatments. It was high in T3 followed by T4. Intercropping system recorded significantly high (P<0.01) cost of cultivation over intercropping system. Among intercropping, T4 resulted maximum net return, monetary equivalent ratio, cost benefit ratio and per day return over other intercropping treatments and sole crops. This experiment revealed that T4 would be the most efficient system in both biological and economical point of view.
{"title":"Biological and Economic Efficiency of Radish (Raphanus sativus L.)Intercropped with Vegetable Amaranthus (Amaranthus tricolor L.)","authors":"T. Seran, I. Brintha","doi":"10.2174/1874840600902010017","DOIUrl":"https://doi.org/10.2174/1874840600902010017","url":null,"abstract":"A field experiment was conducted at the Agronomy farm, Eastern University, Sri Lanka to study the biological and economic efficiency of radish (Raphanus sativus L.) intercropped with vegetable amaranthus (Amaranthus tricolor L.). Treatments were radish as sole crop (T1), vegetable amaranthus as sole crop (T2), 20/50 cm paired row radish with three (T3) or four (T4) rows of vegetable amaranthus in between paired rows and 25/40 cm paired row radish with three (T5) or two (T6) rows of vegetable amaranthus in between paired rows. The results showed that, there was no significant difference (P>0.05) in area time equivalency ratio (ATER) among intercropping treatments and significant differences (P<0.05) were obtained in the land equivalent ratio (LER) and crop performance ratio (CPR). LER (1.31) and CPR (1.71) were superior in T3 and T4 respectively. In case of economic indices, gross return was significantly differed (P<0.01) among treatments. It was high in T3 followed by T4. Intercropping system recorded significantly high (P<0.01) cost of cultivation over intercropping system. Among intercropping, T4 resulted maximum net return, monetary equivalent ratio, cost benefit ratio and per day return over other intercropping treatments and sole crops. This experiment revealed that T4 would be the most efficient system in both biological and economical point of view.","PeriodicalId":166627,"journal":{"name":"The Open Horticulture Journal","volume":"15 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2009-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127307719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2009-03-06DOI: 10.2174/1874840600902010006
H. Luo, S. K. Lee, J. He
In this study, butterhead lettuce (Lactuca sativa L. cv. Baby Butter) plants were grown at three root-zone tem- peratures (RZTs): 25 o C, 30 o C and ambient-RZT (A-RZT) ranging from 26 o C-42 o C while their shoots were maintained at hot ambient temperature ranging from 26 o C-42 o C. Three phosphorus (P) concentrations: -25% P (minus P, 23.25ppm), control (31.00ppm) and +25% P (plus P, 38.75ppm) were supplied to the plants at each RZT using Netherlands Standard Nutrient Solution. Interactions between RZT and P concentrations on productivity, root morphology, maximum photosyn- thetic O2 evolution (Pmax), P uptake and its partitioning between shoot and root were studied. Lettuce plants grown with the plus P concentration at 25 o C-RZT had the highest productivity, highest Pmax, highest shoot and root P concentrations. However, all the root morphological parameters studied were the greatest in plants grown with the minus P at 25 o C-RZT. Higher P concentration in the solution and 25 o C-RZT resulted in higher portion of the absorbed P partitioned to the shoots. Integrated effects of RZT and P concentration on the productivity, root morphology, photosynthesis and P uptake were discussed.
在本研究中，butterhead lettuce (Lactuca sativa L. cv.)在三个根区温度(RZTs): 25℃、30℃和环境温度(A-RZT)范围为26℃~ 42℃的条件下生长，芽部保持在26℃~ 42℃的高温环境温度下。在每个RZT下，使用荷兰标准营养液向植株提供三种磷浓度:-25% P (- P, 23.25ppm)、对照(31.00ppm)和+25% P (+ P, 38.75ppm)。研究了RZT与磷浓度对产量、根系形态、最大光合O2演化(Pmax)、磷吸收及其在地上部和根间分配的相互作用。25 o C-RZT + P处理的生菜产量最高，Pmax最高，地上部和根部磷含量最高。在25℃- rzt负磷处理下生长的植株根系形态参数均最大。溶液中磷浓度越高，25 o C-RZT处理的吸收磷分配到茎部的比例越高。讨论了RZT和P浓度对产量、根系形态、光合作用和P吸收的综合影响。
{"title":"Integrated Effects of Root-Zone Temperatures and Phosphorus Levels on Aeroponically-Grown Lettuce (Lactuca sativa L.) in the Tropics","authors":"H. Luo, S. K. Lee, J. He","doi":"10.2174/1874840600902010006","DOIUrl":"https://doi.org/10.2174/1874840600902010006","url":null,"abstract":"In this study, butterhead lettuce (Lactuca sativa L. cv. Baby Butter) plants were grown at three root-zone tem- peratures (RZTs): 25 o C, 30 o C and ambient-RZT (A-RZT) ranging from 26 o C-42 o C while their shoots were maintained at hot ambient temperature ranging from 26 o C-42 o C. Three phosphorus (P) concentrations: -25% P (minus P, 23.25ppm), control (31.00ppm) and +25% P (plus P, 38.75ppm) were supplied to the plants at each RZT using Netherlands Standard Nutrient Solution. Interactions between RZT and P concentrations on productivity, root morphology, maximum photosyn- thetic O2 evolution (Pmax), P uptake and its partitioning between shoot and root were studied. Lettuce plants grown with the plus P concentration at 25 o C-RZT had the highest productivity, highest Pmax, highest shoot and root P concentrations. However, all the root morphological parameters studied were the greatest in plants grown with the minus P at 25 o C-RZT. Higher P concentration in the solution and 25 o C-RZT resulted in higher portion of the absorbed P partitioned to the shoots. Integrated effects of RZT and P concentration on the productivity, root morphology, photosynthesis and P uptake were discussed.","PeriodicalId":166627,"journal":{"name":"The Open Horticulture Journal","volume":"24 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2009-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121390957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2009-03-06DOI: 10.2174/1874840600902010013
X. Chang, P. Alderson, C. Wright
Essential oil molecules released from fresh leaves of sweet basil (Ocimum basilicum L.) were collected in Tenax traps and analysed using a TD-GC/MS. During daytime from 09.00 to 17.00, there were no differences in the con- tent and composition of total essential oils, however the percentage of eugenol increased slightly. Significant differences were found in both the total content and composition of essential oils between young and mature leaves. The total content in young leaves was four times higher than in mature leaves, with fourfold, twofold and sixtyfold differences in the rela- tive contents of linalool, eugenol and methyl eugenol respectively. There was no difference observed in the relative con- tent of 1,8-cineole. The results therefore suggest that the defining sampling procedure is important when comparing the essential oils between treatments. Key Word: Sweet basil, Ocimum basilicum, essential oil composition, linalool, eugenol, methyl eugenol, 1, 8-cineole.
{"title":"Variation in the essential oils in different leaves of basil (Ocimum basilicum L.) at day time.","authors":"X. Chang, P. Alderson, C. Wright","doi":"10.2174/1874840600902010013","DOIUrl":"https://doi.org/10.2174/1874840600902010013","url":null,"abstract":"Essential oil molecules released from fresh leaves of sweet basil (Ocimum basilicum L.) were collected in Tenax traps and analysed using a TD-GC/MS. During daytime from 09.00 to 17.00, there were no differences in the con- tent and composition of total essential oils, however the percentage of eugenol increased slightly. Significant differences were found in both the total content and composition of essential oils between young and mature leaves. The total content in young leaves was four times higher than in mature leaves, with fourfold, twofold and sixtyfold differences in the rela- tive contents of linalool, eugenol and methyl eugenol respectively. There was no difference observed in the relative con- tent of 1,8-cineole. The results therefore suggest that the defining sampling procedure is important when comparing the essential oils between treatments. Key Word: Sweet basil, Ocimum basilicum, essential oil composition, linalool, eugenol, methyl eugenol, 1, 8-cineole.","PeriodicalId":166627,"journal":{"name":"The Open Horticulture Journal","volume":"82 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2009-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132800360","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2009-03-06DOI: 10.2174/1874840600902010001
W. C. Lin, G. Block
Excised shoot tips of sweet potato (Ipomoea babatas L.) were incubated in H2O2 or NaCl aqueous solution for 24h or 48h prior to a 3-day chilling at 2.5°C. Severity of chilling injury was visually observed during a post-chilling 7-day recovery at 21°C, and scored at 0 to 5 (none to most severe injury). In the first experiment, when cv. Purple (PUR) sweet potato shoots were subjected to 3-day chilling at 2.5°C, a 48h pre-treatment of 150 mM H2O2 under 16h photoperiod re- duced chilling injury, but H2O2 showed no effect under 8h photoperiod. An increase of Oxygen Radical Absorbance Ca- pacity (ORAC) occurred two days after recovery at room temperature, and such increase in ORAC was negatively corre- lated with the severity of chilling injury symptoms observed after seven days at room temperature, indicating the possible protective nature of antioxidants. Because H2O2-reduced chilling injury occurred only on those pre-treated with 16h pho- toperiod, 16h was employed in subsequent NaCl experiments. In the second experiment, 4 cvs were used: Ace of Spades (ACE), B18, Purple (PUR), and Toka Toka Gold (TTG). Each cv formed a 3 x 2 factorial experiment: NaCl (0 mM, 200 mM or 400 mM) and incubation duration (24h or 48h). The effects of NaCl depended on cultivar (cv). NaCl at 200mM reduced chilling injury more for ACE than B18 and PUR, but NaCl increased the injury of TTG. The NaCl effects also depended on incubation duration (24h or 48h). Across 4 cvs the most beneficial NaCl treatment was 200 mM NaCl for 24h. In view of these results, both pre-treatments of H2O2 and NaCl reduced chilling injury of sweet potato shoots, sug- gesting that moderate stress imposed as a pre-treatment increased plant tolerance to subsequent chilling under specific conditions.
{"title":"Chilling injury of sweet potato shoots reduced by prior incubation of H2O2 and NaCl.","authors":"W. C. Lin, G. Block","doi":"10.2174/1874840600902010001","DOIUrl":"https://doi.org/10.2174/1874840600902010001","url":null,"abstract":"Excised shoot tips of sweet potato (Ipomoea babatas L.) were incubated in H2O2 or NaCl aqueous solution for 24h or 48h prior to a 3-day chilling at 2.5°C. Severity of chilling injury was visually observed during a post-chilling 7-day recovery at 21°C, and scored at 0 to 5 (none to most severe injury). In the first experiment, when cv. Purple (PUR) sweet potato shoots were subjected to 3-day chilling at 2.5°C, a 48h pre-treatment of 150 mM H2O2 under 16h photoperiod re- duced chilling injury, but H2O2 showed no effect under 8h photoperiod. An increase of Oxygen Radical Absorbance Ca- pacity (ORAC) occurred two days after recovery at room temperature, and such increase in ORAC was negatively corre- lated with the severity of chilling injury symptoms observed after seven days at room temperature, indicating the possible protective nature of antioxidants. Because H2O2-reduced chilling injury occurred only on those pre-treated with 16h pho- toperiod, 16h was employed in subsequent NaCl experiments. In the second experiment, 4 cvs were used: Ace of Spades (ACE), B18, Purple (PUR), and Toka Toka Gold (TTG). Each cv formed a 3 x 2 factorial experiment: NaCl (0 mM, 200 mM or 400 mM) and incubation duration (24h or 48h). The effects of NaCl depended on cultivar (cv). NaCl at 200mM reduced chilling injury more for ACE than B18 and PUR, but NaCl increased the injury of TTG. The NaCl effects also depended on incubation duration (24h or 48h). Across 4 cvs the most beneficial NaCl treatment was 200 mM NaCl for 24h. In view of these results, both pre-treatments of H2O2 and NaCl reduced chilling injury of sweet potato shoots, sug- gesting that moderate stress imposed as a pre-treatment increased plant tolerance to subsequent chilling under specific conditions.","PeriodicalId":166627,"journal":{"name":"The Open Horticulture Journal","volume":"39 2","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2009-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134260709","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2008-11-21DOI: 10.2174/1874840600801010021
E. Curry
In many deciduous fruit growing regions of the world, because of such factors as lack of chilling hours during winter followed by protracted anthesis, variation in fruit maturity and ripening uniformity of 'Bartlett' (Pyrus Communis) pears is troublesome. Such variability makes timely harvest difficult, especially when labor is insufficient. Use of ReTain ® (aminoethoxyvinylglycine, AVG, Valent Bioscience, Walnut Creek, CA) applied 4 weeks before harvest has mitigated this somewhat by delaying maturity development in treated blocks, thereby providing multiple harvest windows. To fur- ther regulate ripening of ReTain ® -treated 'Bartlett' pears in cold storage, fruit were treated immediately after harvest by dipping fruit in aqueous solutions containing AVG at 0, 66, 132 or 264 mg l -1 and then kept in regular storage at -1 oC. In- ternal ethylene concentration of individual fruit was measured at harvest and monthly thereafter for 4 months. During the first 3 months in storage, ethylene production was well correlated with total (foliar plus dip) AVG applied. Treating 'Bartlett' pears after harvest with AVG appears more efficacious in reducing ripening variability during regular storage such that subsequent increase in ethylene is both consistent and predictable.
在世界上许多落叶水果种植区，由于诸如冬季缺乏冷却时间以及花期延长等因素，“巴特利特”(Pyrus Communis)梨的果实成熟度和成熟均匀性的变化是麻烦的。这种变化使得及时收获变得困难，尤其是在劳动力不足的情况下。在收获前4周使用ReTain®(aminoethoxyvinylglycine, AVG, Valent Bioscience, Walnut Creek, CA)可以通过延迟处理块的成熟发育来减轻这种情况，从而提供多个收获窗口。为了调节冷藏中经过ReTain®处理的‘Bartlett’梨的成熟，在收获后立即将果实浸入含有0、66、132或264 mgl -1的AVG水溶液中进行处理，然后在-1℃下进行常规储存。采收时测定单果内乙烯浓度，此后每月测定一次，连续4个月。在贮藏的前3个月，乙烯产量与施AVG总量(叶面加叶底)呈极显著相关。在收获后用AVG处理“巴特利特”梨，在减少常规储存期间的成熟变异性方面似乎更有效，因此随后乙烯的增加既一致又可预测。
{"title":"Regulating Ripening of ‘Bartlett’ Pears Using Preharvest Plus PostharvestAminoethoxyvinylglycine (AVG)","authors":"E. Curry","doi":"10.2174/1874840600801010021","DOIUrl":"https://doi.org/10.2174/1874840600801010021","url":null,"abstract":"In many deciduous fruit growing regions of the world, because of such factors as lack of chilling hours during winter followed by protracted anthesis, variation in fruit maturity and ripening uniformity of 'Bartlett' (Pyrus Communis) pears is troublesome. Such variability makes timely harvest difficult, especially when labor is insufficient. Use of ReTain ® (aminoethoxyvinylglycine, AVG, Valent Bioscience, Walnut Creek, CA) applied 4 weeks before harvest has mitigated this somewhat by delaying maturity development in treated blocks, thereby providing multiple harvest windows. To fur- ther regulate ripening of ReTain ® -treated 'Bartlett' pears in cold storage, fruit were treated immediately after harvest by dipping fruit in aqueous solutions containing AVG at 0, 66, 132 or 264 mg l -1 and then kept in regular storage at -1 oC. In- ternal ethylene concentration of individual fruit was measured at harvest and monthly thereafter for 4 months. During the first 3 months in storage, ethylene production was well correlated with total (foliar plus dip) AVG applied. Treating 'Bartlett' pears after harvest with AVG appears more efficacious in reducing ripening variability during regular storage such that subsequent increase in ethylene is both consistent and predictable.","PeriodicalId":166627,"journal":{"name":"The Open Horticulture Journal","volume":"16 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2008-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114632115","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2008-10-20DOI: 10.2174/1874840600801010015
A. Yemets, Y. Blume
The results of artificial polyploidization for horticultural and agronomic plant species are presented in this re- view. The data on use of the classical antimitotic drug colchicine for chromosome doubling are reported. The efficacy of other compounds such as dinitroanilines and phosphorothioamidates in polyploidy induction as compared to colchicine has been summarized. The molecular basis of highly specific binding of dinitroanilines and phosphorothioamidates with plant tubulin for induction of efficient polyploidy are discussed.
{"title":"Progress in Plant Polyploidization Based on Antimicrotubular Drugs","authors":"A. Yemets, Y. Blume","doi":"10.2174/1874840600801010015","DOIUrl":"https://doi.org/10.2174/1874840600801010015","url":null,"abstract":"The results of artificial polyploidization for horticultural and agronomic plant species are presented in this re- view. The data on use of the classical antimitotic drug colchicine for chromosome doubling are reported. The efficacy of other compounds such as dinitroanilines and phosphorothioamidates in polyploidy induction as compared to colchicine has been summarized. The molecular basis of highly specific binding of dinitroanilines and phosphorothioamidates with plant tubulin for induction of efficient polyploidy are discussed.","PeriodicalId":166627,"journal":{"name":"The Open Horticulture Journal","volume":"243 8","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2008-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114017561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2008-10-20DOI: 10.2174/1874840600801010001
P. Bhowmik, S. Basu, A. Alam
Fresh asparagus (Asparagus officinalis L.) deteriorates rapidly after harvest. Early enzymatic changes that fol- low harvesting of asparagus are important factors contributing to postharvest deterioration. We held asparagus spears at 25°C for up to 5 days after harvest and examined changes in the activities of Acid invertase (AI), Sucrose Synthase (SS), Glutamine Synthetase (GS) and Phenylalanine ammonia Lyase (PAL) in both top and bottom portions of the spears. Solu- ble AI activity increased during the first day of storage and after that it decreased gradually up to five days in both top and bottom portions. Sucrose synthase activity was higher in the bottom portion than in the top; its activity declined daily for five days. Sucrose content was negatively correlated with invertase and sucrose synthase in both portions.GS enzyme ac- tivity in both top and bottom portion declined throughout the experimental period which may be related to increased am- monia accumulation. The increase in PAL activity continued until day 3 and declined thereafter.
{"title":"Changes in Enzyme Activities During Postharvest Deterioration of GreenAsparagus Spears","authors":"P. Bhowmik, S. Basu, A. Alam","doi":"10.2174/1874840600801010001","DOIUrl":"https://doi.org/10.2174/1874840600801010001","url":null,"abstract":"Fresh asparagus (Asparagus officinalis L.) deteriorates rapidly after harvest. Early enzymatic changes that fol- low harvesting of asparagus are important factors contributing to postharvest deterioration. We held asparagus spears at 25°C for up to 5 days after harvest and examined changes in the activities of Acid invertase (AI), Sucrose Synthase (SS), Glutamine Synthetase (GS) and Phenylalanine ammonia Lyase (PAL) in both top and bottom portions of the spears. Solu- ble AI activity increased during the first day of storage and after that it decreased gradually up to five days in both top and bottom portions. Sucrose synthase activity was higher in the bottom portion than in the top; its activity declined daily for five days. Sucrose content was negatively correlated with invertase and sucrose synthase in both portions.GS enzyme ac- tivity in both top and bottom portion declined throughout the experimental period which may be related to increased am- monia accumulation. The increase in PAL activity continued until day 3 and declined thereafter.","PeriodicalId":166627,"journal":{"name":"The Open Horticulture Journal","volume":"794 ","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2008-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114048588","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2008-10-20DOI: 10.2174/1874840600801010007
G. Calmin, G. Dennler, L. Belbahri, A. Wigger, F. Lefort
This study aimed to assess microbial community diversity in recycled nutrient solutions used in soil-less glass- house cultivation of tomato. One hundred bacterial strains, twenty oomycetes and 6 fungi were isolated and identified through genomic DNA isolation, PCR amplification of the ribosomal DNA region and database interrogations. Similari- ties of ITS regions with known species ranged from 95% to 100%. This artificial ecosystem was shown to be microbio- logically diverse, since recovered isolate were close to 35 bacterial species, 11 oomycete species and 3 fungal species. Bacteria belonged almost exclusively to � -Proteobacteria and Firmicutes, with most represented genera being Bacillus, Acinetobacter, Klebsiella and Serratia. A few bacterial sequences grouped with clones similar to plant and human patho- gens, while other isolates could be protective bacteria such as Pseudomonas fluorescens. Oomycetes isolated mostly be- longed to the genus Pythium (19 isolates) and were phylogenetically related to common cosmopolitan soil inhabitants or phytopathogenic Pythium species. The six fungal isolates were in 2 genera, Rhizopus and Caesia; Rhizopus isolates were closely related to the post harvest pathogen Rhizopus stolonifer. This original work adds to the efforts of assessing micro- organism diversity in recycled nutrient solutions commonly used in glasshouse vegetable production; microbial diversity was high and included potential plant pathogens. This study demonstrated the existence of a wide cultivable microbial community in the nutrient solution before recycling and recirculation and supported the necessity for disinfecting nutrient solutions used in soil-less cultivation systems, during the recycling process, in order to ensure crop sanitation and avoid- ing plant disease spreading.
{"title":"Molecular Identification of Microbial Communities in the Recycled Nutrient Solution of a Tomato Glasshouse Soil-Less Culture","authors":"G. Calmin, G. Dennler, L. Belbahri, A. Wigger, F. Lefort","doi":"10.2174/1874840600801010007","DOIUrl":"https://doi.org/10.2174/1874840600801010007","url":null,"abstract":"This study aimed to assess microbial community diversity in recycled nutrient solutions used in soil-less glass- house cultivation of tomato. One hundred bacterial strains, twenty oomycetes and 6 fungi were isolated and identified through genomic DNA isolation, PCR amplification of the ribosomal DNA region and database interrogations. Similari- ties of ITS regions with known species ranged from 95% to 100%. This artificial ecosystem was shown to be microbio- logically diverse, since recovered isolate were close to 35 bacterial species, 11 oomycete species and 3 fungal species. Bacteria belonged almost exclusively to � -Proteobacteria and Firmicutes, with most represented genera being Bacillus, Acinetobacter, Klebsiella and Serratia. A few bacterial sequences grouped with clones similar to plant and human patho- gens, while other isolates could be protective bacteria such as Pseudomonas fluorescens. Oomycetes isolated mostly be- longed to the genus Pythium (19 isolates) and were phylogenetically related to common cosmopolitan soil inhabitants or phytopathogenic Pythium species. The six fungal isolates were in 2 genera, Rhizopus and Caesia; Rhizopus isolates were closely related to the post harvest pathogen Rhizopus stolonifer. This original work adds to the efforts of assessing micro- organism diversity in recycled nutrient solutions commonly used in glasshouse vegetable production; microbial diversity was high and included potential plant pathogens. This study demonstrated the existence of a wide cultivable microbial community in the nutrient solution before recycling and recirculation and supported the necessity for disinfecting nutrient solutions used in soil-less cultivation systems, during the recycling process, in order to ensure crop sanitation and avoid- ing plant disease spreading.","PeriodicalId":166627,"journal":{"name":"The Open Horticulture Journal","volume":"17 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2008-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116783910","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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