Parisa Ahmadi, H. Alavi-Majd, S. Khodakarim, Leili Tapak, N. Kariman, Payam Amini, Forough Pazhuheian
Low birth weight (neonate weighing less than 2500 g) is associated with several maternal and fetal factors, all interrelated with each other [ 1 ]. This study is aimed to survey maternal risk factors associated with low birth weight neonates using data mining (Random Forest) to account for interactions between them. We also intended to compare Random Forest with traditional Logistic regression. The dataset used in the present study consisted of 600 volunteer pregnant women. This cross-sectional study was carried out in Milad hospital, Tehran, during 2005-2009. Ten potential risk factors that are commonly associated with low birth weight were selected by using Random Forest technique. Several criteria such as the area under ROC curve were considered in comparing Random Forest with Logistic Regression.According to both criteria, four top rank variables identified by Random Forest were pregnancy age, body mass index during the third three months of pregnancy, mother’s age and body mass index during the first three months of pregnancy, respectively. In addition, in terms of different criteria the Random Forest technique outperformed the Logistic regression (area under ROC curve: 93% ; Total Accuracy:95% ; Kappa Coefficient: 66%).The results of the present study showed that using Random Forest improved the prediction of low birth weight compared with Logistic Regression. This is because of the fact that the former accounts for all interactions between covariates. Therefore, this approach is a promising classifier for predicting low birth weight .
{"title":"Prediction of low birth weight using Random Forest: A comparison with Logistic Regression","authors":"Parisa Ahmadi, H. Alavi-Majd, S. Khodakarim, Leili Tapak, N. Kariman, Payam Amini, Forough Pazhuheian","doi":"10.22037/JPS.V8I3.15412","DOIUrl":"https://doi.org/10.22037/JPS.V8I3.15412","url":null,"abstract":"Low birth weight (neonate weighing less than 2500 g) is associated with several maternal and fetal factors, all interrelated with each other [ 1 ]. This study is aimed to survey maternal risk factors associated with low birth weight neonates using data mining (Random Forest) to account for interactions between them. We also intended to compare Random Forest with traditional Logistic regression. The dataset used in the present study consisted of 600 volunteer pregnant women. This cross-sectional study was carried out in Milad hospital, Tehran, during 2005-2009. Ten potential risk factors that are commonly associated with low birth weight were selected by using Random Forest technique. Several criteria such as the area under ROC curve were considered in comparing Random Forest with Logistic Regression.According to both criteria, four top rank variables identified by Random Forest were pregnancy age, body mass index during the third three months of pregnancy, mother’s age and body mass index during the first three months of pregnancy, respectively. In addition, in terms of different criteria the Random Forest technique outperformed the Logistic regression (area under ROC curve: 93% ; Total Accuracy:95% ; Kappa Coefficient: 66%).The results of the present study showed that using Random Forest improved the prediction of low birth weight compared with Logistic Regression. This is because of the fact that the former accounts for all interactions between covariates. Therefore, this approach is a promising classifier for predicting low birth weight .","PeriodicalId":16663,"journal":{"name":"Journal of paramedical sciences","volume":"73 1","pages":"36-43"},"PeriodicalIF":0.0,"publicationDate":"2017-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88750268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
F. Kazerouni, Hoda Zeynalian, F. Ebrahimi, A. Rahimipour, Mostafa Bakhshi, Roghaieh Samadi
Production of antibodies in chickens (IgY) has significantly attracted attention of scientists. Numerous publications have reported use of IgY in diagnosis, therapy and prophylaxis. Production of antigen-specific antibodies in chicken can help treat and prevent infectious diseases. The aim of this study was to assess the safety of IgY( anti E. coli O157:H7) on the antioxidant system in mice .Therefore in this study, three different doses of IgY against E. coli O157:H7 (0.9375, 1.875 and 3.75 g / kg) were administrated through oral route to 18 mice (treated groups) and PBS to the control group and 14 days after administration, blood samples were collected from the mice. Serum malondialdehyde (MDA) level and catalase, glutathione peroxidase and superoxide dismutase activity were measured using commercial kits. Oral administration of IgY against E. coli O157:H7 in doses of 0.9375, 1.875 and 3.75 g / kg caused no deaths and showed no toxic effects on mice. In this study, after 14 days of IgY administration there were no significant changes in the activity of antioxidant enzymes (catalase, glutathione peroxidase and superoxide dismutase) and MDA serum level compared to the control group. Our findings revealed that oral administration of IgY against E. coli O157:H7 does not show any toxic effects and does not disturb the antioxidant system in mice . These findings could be indicative of safety of oral administration of IgY in mice.
鸡体内产生的抗体(IgY)引起了科学家们的极大关注。许多出版物报道了IgY在诊断、治疗和预防中的应用。鸡体内产生抗原特异性抗体有助于治疗和预防传染病。为了研究抗O157:H7大肠杆菌IgY对小鼠抗氧化系统的安全性，本研究采用三种不同剂量的抗O157:H7大肠杆菌IgY(0.9375、1.875和3.75 g / kg)分别口服给药组(18只)和对照组(PBS)，给药后14 d采集小鼠血液。测定血清丙二醛(MDA)水平及过氧化氢酶、谷胱甘肽过氧化物酶和超氧化物歧化酶活性。口服剂量分别为0.9375、1.875和3.75 g / kg的IgY抗大肠杆菌O157:H7，未造成小鼠死亡，对小鼠无毒性作用。在本研究中，IgY给药14天后，抗氧化酶(过氧化氢酶、谷胱甘肽过氧化物酶和超氧化物歧化酶)活性和血清丙二醛水平与对照组相比无显著变化。我们的研究结果表明，口服IgY抗大肠杆菌O157:H7没有任何毒性作用，也不会干扰小鼠的抗氧化系统。这些发现可能表明口服IgY小鼠的安全性。
{"title":"Assessment of the safety of chicken egg yolk antibody (IgY) consumption by lipid peroxidation marker in mice","authors":"F. Kazerouni, Hoda Zeynalian, F. Ebrahimi, A. Rahimipour, Mostafa Bakhshi, Roghaieh Samadi","doi":"10.22037/JPS.V8I3.17032","DOIUrl":"https://doi.org/10.22037/JPS.V8I3.17032","url":null,"abstract":"Production of antibodies in chickens (IgY) has significantly attracted attention of scientists. Numerous publications have reported use of IgY in diagnosis, therapy and prophylaxis. Production of antigen-specific antibodies in chicken can help treat and prevent infectious diseases. The aim of this study was to assess the safety of IgY( anti E. coli O157:H7) on the antioxidant system in mice .Therefore in this study, three different doses of IgY against E. coli O157:H7 (0.9375, 1.875 and 3.75 g / kg) were administrated through oral route to 18 mice (treated groups) and PBS to the control group and 14 days after administration, blood samples were collected from the mice. Serum malondialdehyde (MDA) level and catalase, glutathione peroxidase and superoxide dismutase activity were measured using commercial kits. Oral administration of IgY against E. coli O157:H7 in doses of 0.9375, 1.875 and 3.75 g / kg caused no deaths and showed no toxic effects on mice. In this study, after 14 days of IgY administration there were no significant changes in the activity of antioxidant enzymes (catalase, glutathione peroxidase and superoxide dismutase) and MDA serum level compared to the control group. Our findings revealed that oral administration of IgY against E. coli O157:H7 does not show any toxic effects and does not disturb the antioxidant system in mice . These findings could be indicative of safety of oral administration of IgY in mice.","PeriodicalId":16663,"journal":{"name":"Journal of paramedical sciences","volume":"1 1","pages":"29-35"},"PeriodicalIF":0.0,"publicationDate":"2017-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85540232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Exposure rate constant (G) relates activity of a point source to exposure rate at certain distance. It’s an important parameter in radiation protection, medical uses and radiological assessments. In this study, exposure rate constant for three radionuclides include 60 Co, 22 Na and 111 In were calculated with FLUKA Monte Carlo method. Each source was simulated and exposure rate at different distances of 20, 30, 40, 60, 80, 100, 120, 150, 200 and 300cm from the sources were measured. For dose measurements, a farmer ionization chamber was simulated with active volume of 0.6cm 3 , inner electrode of aluminium with diameter of1mm and wall of pure graphite with thickness of 0.75mm. Calculated exposure rate constants for 60 Co, 22 Na and 111 In at 20 to 300cm from the source ranged from 33.45 to 0.16 mR/(mCi.h), 29.12 to 0.10 mR/(mCi.h) and 8.73 to 0.03 mR/(mCi.h) respectively which show a decreasing trend. Comparison of our results with other studies show that there are good agreement for 60 Co and 22 Na; the present values for exposure rate constant for 111 In was higher than previous reports.
{"title":"Calculation of exposure rate constant for 60Co, 22Na and 111In Sources with FLUKA Monte Carlo Code","authors":"Daryoush Khoramian, V. Saba","doi":"10.22037/JPS.V8I3.12143","DOIUrl":"https://doi.org/10.22037/JPS.V8I3.12143","url":null,"abstract":"Exposure rate constant (G) relates activity of a point source to exposure rate at certain distance. It’s an important parameter in radiation protection, medical uses and radiological assessments. In this study, exposure rate constant for three radionuclides include 60 Co, 22 Na and 111 In were calculated with FLUKA Monte Carlo method. Each source was simulated and exposure rate at different distances of 20, 30, 40, 60, 80, 100, 120, 150, 200 and 300cm from the sources were measured. For dose measurements, a farmer ionization chamber was simulated with active volume of 0.6cm 3 , inner electrode of aluminium with diameter of1mm and wall of pure graphite with thickness of 0.75mm. Calculated exposure rate constants for 60 Co, 22 Na and 111 In at 20 to 300cm from the source ranged from 33.45 to 0.16 mR/(mCi.h), 29.12 to 0.10 mR/(mCi.h) and 8.73 to 0.03 mR/(mCi.h) respectively which show a decreasing trend. Comparison of our results with other studies show that there are good agreement for 60 Co and 22 Na; the present values for exposure rate constant for 111 In was higher than previous reports.","PeriodicalId":16663,"journal":{"name":"Journal of paramedical sciences","volume":"448 1","pages":"18-21"},"PeriodicalIF":0.0,"publicationDate":"2017-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82908350","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
H. Zarei, H. Mozdarani, A. Mahmoudzadeh, Mohsen Bakhshandeh, N. Nayeri
The most important problem with radiotherapy is the limitation of whole body irradiation of a metastatic patient. There are evidence showing that similar effect will occur in non-irradiated tumors similar to the irradiated ones. This effect is called abscopal effect. In the present study, the abscopal effect on local induced mice breast cancers has been investigated. One million of 4T1 mice breast cancer cell line was injected to balb/c mice subcutaneously while being under anesthesia. After the growth of tumors till becoming palpable, one of two induced tumors were exposed to total 28 Gy, with gamma rays emitted from a cobalt -60 tele-therapy machine in 14 fractions with 2 Gy daily doses. Tumor volumes were measured, using the caliper. The data was analyzed by the use of non-parametrical and ANOVA tests. Similar growth in non-irradiated control tumors was seen. After 10 or 11 fractions of one- side irradiation and total dose of 20 to 22 Gy, however, non-irradiated tumors, similar to irradiated ones, showed similar effect, reduction of size and volume different from control groups (P<0.05).The reduction of non-irradiated tumors relative to irradiation of another tumor in the same mouse is the emblem of occurrence of the abscopal effect. The mechanism of the abscopal effect is unknown but it could be related to the release of cytokines from irradiated tumors with their receptors existing on the surface of non-irradiated tumors. By induction of this effect, the remission probability of small metastases after local irradiation could be possible.
{"title":"The probability of influence of the abscopal effect on reduction of similar far away 4T1 cell line tumors by irradiation of main tumor","authors":"H. Zarei, H. Mozdarani, A. Mahmoudzadeh, Mohsen Bakhshandeh, N. Nayeri","doi":"10.22037/JPS.V8I3.12674","DOIUrl":"https://doi.org/10.22037/JPS.V8I3.12674","url":null,"abstract":"The most important problem with radiotherapy is the limitation of whole body irradiation of a metastatic patient. There are evidence showing that similar effect will occur in non-irradiated tumors similar to the irradiated ones. This effect is called abscopal effect. In the present study, the abscopal effect on local induced mice breast cancers has been investigated. One million of 4T1 mice breast cancer cell line was injected to balb/c mice subcutaneously while being under anesthesia. After the growth of tumors till becoming palpable, one of two induced tumors were exposed to total 28 Gy, with gamma rays emitted from a cobalt -60 tele-therapy machine in 14 fractions with 2 Gy daily doses. Tumor volumes were measured, using the caliper. The data was analyzed by the use of non-parametrical and ANOVA tests. Similar growth in non-irradiated control tumors was seen. After 10 or 11 fractions of one- side irradiation and total dose of 20 to 22 Gy, however, non-irradiated tumors, similar to irradiated ones, showed similar effect, reduction of size and volume different from control groups (P<0.05).The reduction of non-irradiated tumors relative to irradiation of another tumor in the same mouse is the emblem of occurrence of the abscopal effect. The mechanism of the abscopal effect is unknown but it could be related to the release of cytokines from irradiated tumors with their receptors existing on the surface of non-irradiated tumors. By induction of this effect, the remission probability of small metastases after local irradiation could be possible.","PeriodicalId":16663,"journal":{"name":"Journal of paramedical sciences","volume":"30 1","pages":"22-28"},"PeriodicalIF":0.0,"publicationDate":"2017-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83828737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Aghaei, A. Farahnak, T. Golmohamadi, M. Eshraghian, Mohammad-bagher Molaei-Rad
The present work was designed to determine alkaline phosphatase (ALP) activity level in Fasciola hepatica parasite and Fasciola infected livers to evaluate the effects of liver infection on enzyme activity and to compare enzyme activity in liver and parasite. The sheep livers were collected and adult Fasciola hepatica parasites were isolated and washed with PBS buffer. Collected healthy and infected livers and parasite were homogenized and extract solutions were centrifuged and stored at -20°C. ALP enzymes activity was measured in the extract solution of samples. Proteins of the samples were measured and protein bands were detected through using Bradford and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) methods respectively. Independent two samples t-test was performed to determine significant difference between different groups. The mean values of the assayed ALP specific activities of infected and healthy livers and Fasciola hepatica parasite were estimated 0.163,0.133,0.048 U/mg protein/ml correspondingly. Gel electrophoresis (SDS-PAGE) of Fasciola hepatica and liver extract solution samples displayed different protein bands, including ALP enzyme. Statistical analysis did not show significant difference between enzyme’s activity in infected and healthy livers (P>0.05). However, the liver demonstrated more than ALP activity level to parasite (P<0.05). The findings show that ALP enzymes activity in Fasciola hepatica infected livers could not be concerned as a specific pathological biomarker in fascioliasis, but meanwhile this enzyme displays interest activity in parasite.
{"title":"In vitro assay of alkaline phosphatase enzyme activity in liver and fasciola hepatica parasite","authors":"S. Aghaei, A. Farahnak, T. Golmohamadi, M. Eshraghian, Mohammad-bagher Molaei-Rad","doi":"10.22037/JPS.V8I3.11560","DOIUrl":"https://doi.org/10.22037/JPS.V8I3.11560","url":null,"abstract":"The present work was designed to determine alkaline phosphatase (ALP) activity level in Fasciola hepatica parasite and Fasciola infected livers to evaluate the effects of liver infection on enzyme activity and to compare enzyme activity in liver and parasite. The sheep livers were collected and adult Fasciola hepatica parasites were isolated and washed with PBS buffer. Collected healthy and infected livers and parasite were homogenized and extract solutions were centrifuged and stored at -20°C. ALP enzymes activity was measured in the extract solution of samples. Proteins of the samples were measured and protein bands were detected through using Bradford and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) methods respectively. Independent two samples t-test was performed to determine significant difference between different groups. The mean values of the assayed ALP specific activities of infected and healthy livers and Fasciola hepatica parasite were estimated 0.163,0.133,0.048 U/mg protein/ml correspondingly. Gel electrophoresis (SDS-PAGE) of Fasciola hepatica and liver extract solution samples displayed different protein bands, including ALP enzyme. Statistical analysis did not show significant difference between enzyme’s activity in infected and healthy livers (P>0.05). However, the liver demonstrated more than ALP activity level to parasite (P<0.05). The findings show that ALP enzymes activity in Fasciola hepatica infected livers could not be concerned as a specific pathological biomarker in fascioliasis, but meanwhile this enzyme displays interest activity in parasite.","PeriodicalId":16663,"journal":{"name":"Journal of paramedical sciences","volume":"26 1","pages":"13-17"},"PeriodicalIF":0.0,"publicationDate":"2017-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85287982","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Serotonin exerts an inhibitory effect on food intakes. D-Lys3 -GHRP-6 is a ghrelin receptor antagonist which inhibits fasting or ghrelin- induced food intake. In the present study, the effects of serotonin on food intakes were investigated in D-Lys 3 - GHRP-6- treated food-deprived male rats. Twenty food-deprived male rats in four groups (5 in each) received third cerebral ventricular injection of saline, 10µg serotonin, 20nmol D-Lys 3 -GHRP-6 or combination of D-Lys 3 -GHRP-6 and serotonin respectively. Mean of one hour food intakes were measured. Data was analyzed by one-way analysis of variance (ANOVA) with post hoc Tukey’s test. In all cases, P<0.05 was considered to be statistically significant. Serotonin, D-Lys 3 - GHRP-6 or combination of serotonin/D-Lys 3 - GHRP-6 decreased the mean food intakes significantly when compared to saline. A significant decrease was observed on mean food intakes between combination of D-Lys 3 -GHRP-6 and serotonin group and serotonin or D-Lys 3 - GHRP-6 groups. A significant decrease was not observed on food intakes between serotonin and D-Lys 3 - GHRP-6 groups. Serotonin and D-Lys 3 -GHRP-6 may exert synergistic inhibitory effects on food intake in rats. Decrease of the ghrelin pathway activity may have a role in mediating the inhibitory effects of serotonin on food intakes.
{"title":"Synergistic effects of serotonin and D-lys3- GHRP-6 on food intakes in food deprived male rats","authors":"F. Mahmoudi","doi":"10.22037/JPS.V8I3.15784","DOIUrl":"https://doi.org/10.22037/JPS.V8I3.15784","url":null,"abstract":"Serotonin exerts an inhibitory effect on food intakes. D-Lys3 -GHRP-6 is a ghrelin receptor antagonist which inhibits fasting or ghrelin- induced food intake. In the present study, the effects of serotonin on food intakes were investigated in D-Lys 3 - GHRP-6- treated food-deprived male rats. Twenty food-deprived male rats in four groups (5 in each) received third cerebral ventricular injection of saline, 10µg serotonin, 20nmol D-Lys 3 -GHRP-6 or combination of D-Lys 3 -GHRP-6 and serotonin respectively. Mean of one hour food intakes were measured. Data was analyzed by one-way analysis of variance (ANOVA) with post hoc Tukey’s test. In all cases, P<0.05 was considered to be statistically significant. Serotonin, D-Lys 3 - GHRP-6 or combination of serotonin/D-Lys 3 - GHRP-6 decreased the mean food intakes significantly when compared to saline. A significant decrease was observed on mean food intakes between combination of D-Lys 3 -GHRP-6 and serotonin group and serotonin or D-Lys 3 - GHRP-6 groups. A significant decrease was not observed on food intakes between serotonin and D-Lys 3 - GHRP-6 groups. Serotonin and D-Lys 3 -GHRP-6 may exert synergistic inhibitory effects on food intake in rats. Decrease of the ghrelin pathway activity may have a role in mediating the inhibitory effects of serotonin on food intakes.","PeriodicalId":16663,"journal":{"name":"Journal of paramedical sciences","volume":"44 1","pages":"9-12"},"PeriodicalIF":0.0,"publicationDate":"2017-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87347539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Fatemeh Taghavirad, M. Hamidpour, M. Soleimani, Abass Hjifathali
Hematopoietic stem cells (HSC), are considered as an important source for HSCT transplantation. There are several regulators for stem cells migration and homing, among which the nervous system is an important one. This study is going to investigate the role of nervous system in homing of hematopoietic stem cells. In an experimental study, mononuclear cells were isolated from peripheral blood and treated by a beta adrenergic receptors blocker (Sotalol). After treating the cells with different concentrations 0.5- 500 (mM/ml) for 1- 24 hours, RNA was extracted and the expression of VLA4, VLA5 and CXCR4 genes were determinated through RT-PCR method.The results of this study demonstrated high expression in genes associated with homing of the cells after being treated with drug for 1 hour: CXCR4 gene expression increases in 10 and 50 mM/ml, VLA4 gene expression in 50mM/ml and VLA5 gene expression in 10 mM/ml concentration of Sotalol.Using beta adrenergic receptors blockers in appropriate time and dose of drug can affect the expression of genes which involved in HSCs homing and will lead to high success rate of transplantation of these cells.
{"title":"Evaluation of CXCR4, VLA4, and VLA5 expression in peripheral mobilized hematopoietic stem cells in presence of Sotalol","authors":"Fatemeh Taghavirad, M. Hamidpour, M. Soleimani, Abass Hjifathali","doi":"10.22037/JPS.V8I3.15233","DOIUrl":"https://doi.org/10.22037/JPS.V8I3.15233","url":null,"abstract":"Hematopoietic stem cells (HSC), are considered as an important source for HSCT transplantation. There are several regulators for stem cells migration and homing, among which the nervous system is an important one. This study is going to investigate the role of nervous system in homing of hematopoietic stem cells. In an experimental study, mononuclear cells were isolated from peripheral blood and treated by a beta adrenergic receptors blocker (Sotalol). After treating the cells with different concentrations 0.5- 500 (mM/ml) for 1- 24 hours, RNA was extracted and the expression of VLA4, VLA5 and CXCR4 genes were determinated through RT-PCR method.The results of this study demonstrated high expression in genes associated with homing of the cells after being treated with drug for 1 hour: CXCR4 gene expression increases in 10 and 50 mM/ml, VLA4 gene expression in 50mM/ml and VLA5 gene expression in 10 mM/ml concentration of Sotalol.Using beta adrenergic receptors blockers in appropriate time and dose of drug can affect the expression of genes which involved in HSCs homing and will lead to high success rate of transplantation of these cells.","PeriodicalId":16663,"journal":{"name":"Journal of paramedical sciences","volume":"260 1","pages":"1-8"},"PeriodicalIF":0.0,"publicationDate":"2017-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86971102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
H. Moghaddasi, R. Rabiei, F. Asadi, A. Mohammadpour
National Health Information Network (NHIN) is a network in which all health care organizations, government agencies and other health-related organizations are connected to each other to exchange information about health. Due to the necessity of a framework for NHIN development, in this paper, according to the literature review, a definition for NHIN framework was provided, and then the NHIN related projects were reviewed in the United States of America (USA) and the United Kingdom (UK), NHIN and National Programme for Information Technology in the NHS (NPfIT), respectively. The Review of NHIN framework in the countries studied show some similarities and differences in each dimension that are discussed in this framework. NHIN guiding principles in the NHS NPfIT were not regarded or were considered incomplete, compared to the US. NHIN architecture in the US is decentralized while it is centralized in the UK. Based on the review of NHIN framework, these two countries represent important points that can be used in many other countries. However, it can be said that the development of NHIN does not only mean the implementation of national system or systems, or the binding of local health information systems, but It also needs to build on a framework in which many of the issues related to the formation of NHIN would be considered; including the cooperation between government, private sector and stakeholders with regard to local, national and international needs.
{"title":"National Health Information Network: Lessons Learned From the USA and the UK","authors":"H. Moghaddasi, R. Rabiei, F. Asadi, A. Mohammadpour","doi":"10.22037/JPS.V8I2.15209","DOIUrl":"https://doi.org/10.22037/JPS.V8I2.15209","url":null,"abstract":"National Health Information Network (NHIN) is a network in which all health care organizations, government agencies and other health-related organizations are connected to each other to exchange information about health. Due to the necessity of a framework for NHIN development, in this paper, according to the literature review, a definition for NHIN framework was provided, and then the NHIN related projects were reviewed in the United States of America (USA) and the United Kingdom (UK), NHIN and National Programme for Information Technology in the NHS (NPfIT), respectively. The Review of NHIN framework in the countries studied show some similarities and differences in each dimension that are discussed in this framework. NHIN guiding principles in the NHS NPfIT were not regarded or were considered incomplete, compared to the US. NHIN architecture in the US is decentralized while it is centralized in the UK. Based on the review of NHIN framework, these two countries represent important points that can be used in many other countries. However, it can be said that the development of NHIN does not only mean the implementation of national system or systems, or the binding of local health information systems, but It also needs to build on a framework in which many of the issues related to the formation of NHIN would be considered; including the cooperation between government, private sector and stakeholders with regard to local, national and international needs.","PeriodicalId":16663,"journal":{"name":"Journal of paramedical sciences","volume":"2 1","pages":"57-70"},"PeriodicalIF":0.0,"publicationDate":"2017-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82673691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ebola is one of the members of filoviruses family. It causes severe hemorrhagic with the human mortality rate of 50-90%. In the initial steps of infection, it enters the host cell by Glyco protein1 (GP1). GP1 is a big subunit with the molecular weight of 130 kDa. Its N-terminal domain is responsible for attaching the host cell. In the present study, a database containing 100.000 drug like chemicals which was obtained from Zinc has been screened. The top successive hits were then analyzed regarding Lipinski rules, oral toxicity value and recorded biological properties. Finally, 3 new ligands were introduced as new theoretical inhibitors of Ebola virus entry. Hit #1 (Heptacyclo[18.7.0.02,10.03,8.011,19.012,17.021,26] heptacosa1(20),2(10),3,5,7,11(19),12,14,16,21,23,25-didecaene-9,18,27-trione) indicated the binding affinity of -10.7 kcal/mol with previous biological reports indicating no toxicity on human cell line. Hit#3 (1,1',3,3'-tetraethyl-5'-(4-nitrophenyl)spiro[1,3-diazinane-5,6'-5H-furo[2,3-d]pyrimidine]-2,2',4,4',6 pentone) with the binding affinity of -9.7 kcal/mol theoretically passed pharmacological filters and the toxicity class of 4 theoretically revealed that this hit also can be a potential anti GP1 agent. Moreover, hit#4(10-(Hydroxymethyl)-7,18-dimethoxy-6,17-dimethyl-21-methyl-11.21 diazapentacyclo[11.7.1.02,11.04,9.015,20]henicosa-4(9),6,15(20),17-tetraene-5,8,12,16,19-pentone) also indicated the binding affinity of -9.7 kcal/mol and was matched with Lipinski rules and the toxicity class 4, suggesting that it can be safe in low dose usage. Based on the pharmacological properties, hit #1, 3 and 4 are presented as the new hypothetical drugs against GP1. Moreover, hit#1 has previously been recorded as nontoxic activity on human cell line and makes it an appropriate candidate for further in vitro and in vivo studies.
{"title":"Ligand screening approach to find potential inhibitors of GP1 from Ebola Virus","authors":"G. Rigi, R. Armand, Ebrahim Torktaz","doi":"10.22037/JPS.V8I2.13549","DOIUrl":"https://doi.org/10.22037/JPS.V8I2.13549","url":null,"abstract":"Ebola is one of the members of filoviruses family. It causes severe hemorrhagic with the human mortality rate of 50-90%. In the initial steps of infection, it enters the host cell by Glyco protein1 (GP1). GP1 is a big subunit with the molecular weight of 130 kDa. Its N-terminal domain is responsible for attaching the host cell. In the present study, a database containing 100.000 drug like chemicals which was obtained from Zinc has been screened. The top successive hits were then analyzed regarding Lipinski rules, oral toxicity value and recorded biological properties. Finally, 3 new ligands were introduced as new theoretical inhibitors of Ebola virus entry. Hit #1 (Heptacyclo[18.7.0.02,10.03,8.011,19.012,17.021,26] heptacosa1(20),2(10),3,5,7,11(19),12,14,16,21,23,25-didecaene-9,18,27-trione) indicated the binding affinity of -10.7 kcal/mol with previous biological reports indicating no toxicity on human cell line. Hit#3 (1,1',3,3'-tetraethyl-5'-(4-nitrophenyl)spiro[1,3-diazinane-5,6'-5H-furo[2,3-d]pyrimidine]-2,2',4,4',6 pentone) with the binding affinity of -9.7 kcal/mol theoretically passed pharmacological filters and the toxicity class of 4 theoretically revealed that this hit also can be a potential anti GP1 agent. Moreover, hit#4(10-(Hydroxymethyl)-7,18-dimethoxy-6,17-dimethyl-21-methyl-11.21 diazapentacyclo[11.7.1.02,11.04,9.015,20]henicosa-4(9),6,15(20),17-tetraene-5,8,12,16,19-pentone) also indicated the binding affinity of -9.7 kcal/mol and was matched with Lipinski rules and the toxicity class 4, suggesting that it can be safe in low dose usage. Based on the pharmacological properties, hit #1, 3 and 4 are presented as the new hypothetical drugs against GP1. Moreover, hit#1 has previously been recorded as nontoxic activity on human cell line and makes it an appropriate candidate for further in vitro and in vivo studies.","PeriodicalId":16663,"journal":{"name":"Journal of paramedical sciences","volume":"1 1","pages":"31-36"},"PeriodicalIF":0.0,"publicationDate":"2017-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84727712","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Tyrosinase (Ec: 1.14.18.1) is a copper - containing enzyme which is distributed in all domains of life such as prokaryote, eukaryote, mammals, invertebrates and plants. Tyrosinase catalyzes the oxidation of monophenols to diphenols and diphenols to o-quinones . The tyrosinase crystallographic data shows two histidine -rich regions named CuA and CuB. A loop containing residues M374, S375 and V377 connects the CuA and CuB Centers. This loop is essential for stability of the enzyme. In this study, site directed mutagenesis was used for the replacement of M374 by Arginine and Lysine.in synthesized cDNA cloned in pET 28b (+) . These mutations don't affect the orientation of the Histidin 367(H367) side chain, resulting in loss of activity.
{"title":"Loss of Human Tyrosinase DOPA Oxidase Activity in Artificial M374 Arg and M 374 Lys Mutants","authors":"Roudabeh Behzadi Andouhjerdi, M. Sadeghizadeh","doi":"10.22037/JPS.V8I2.15240","DOIUrl":"https://doi.org/10.22037/JPS.V8I2.15240","url":null,"abstract":"Tyrosinase (Ec: 1.14.18.1) is a copper - containing enzyme which is distributed in all domains of life such as prokaryote, eukaryote, mammals, invertebrates and plants. Tyrosinase catalyzes the oxidation of monophenols to diphenols and diphenols to o-quinones . The tyrosinase crystallographic data shows two histidine -rich regions named CuA and CuB. A loop containing residues M374, S375 and V377 connects the CuA and CuB Centers. This loop is essential for stability of the enzyme. In this study, site directed mutagenesis was used for the replacement of M374 by Arginine and Lysine.in synthesized cDNA cloned in pET 28b (+) . These mutations don't affect the orientation of the Histidin 367(H367) side chain, resulting in loss of activity.","PeriodicalId":16663,"journal":{"name":"Journal of paramedical sciences","volume":"155-156 1","pages":"50-56"},"PeriodicalIF":0.0,"publicationDate":"2017-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73169740","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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