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miR-26a-5p Attenuates Oxidative Stress and Inflammation in Diabetic Retinopathy through the USP14/NF-κB Signaling Pathway miR-26a-5p 通过 USP14/NF-κB 信号通路减轻糖尿病视网膜病变中的氧化应激和炎症反应
IF 1.9 4区 医学 Q3 OPHTHALMOLOGY Pub Date : 2024-01-19 DOI: 10.1155/2024/1470898
Jie Bian, Weizhong Ge, Zhengmei Jiang
<i>Purpose</i>. Diabetic retinopathy (DR) is an ocular disease caused by diabetes and may lead to vision impairment and even blindness. Oxidative stress and inflammation are two key pathogenic factors of DR. Recently, regulatory roles of different microRNAs (miRNAs) in DR have been widely verified. miR-26a-5p has been confirmed to be a potential biomarker of DR. Nevertheless, the specific functions of miR-26a-5p in DR are still unclear. <i>Methods</i>. Primary cultured mouse retinal Müller cells in exposure to high glucose (HG) were used to establish an <i>in vitro</i> DR model. Müller cells were identified via morphology observation under phase contrast microscope and fluorescence staining for glutamine synthetase. The <i>in vivo</i> animal models for DR were constructed using streptozotocin-induced diabetic C57BL/6 mice. Western blotting was performed to quantify cytochrome c protein level in the cytoplasm and mitochondria of Müller cells and to measure protein levels of glial fibrillary acidic protein (GFAP), ubiquitin-specific peptidase 14 (USP14), as well as factors associated with NF-<i>κ</i>B signaling (p-I<i>κ</i>B<i>α</i>, I<i>κ</i>B<i>α</i>, p-<span><svg height="11.4781pt" style="vertical-align:-3.12928pt" version="1.1" viewbox="-0.0498162 -8.34882 19.464 11.4781" width="19.464pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,0,0)"></path></g><g transform="matrix(.013,0,0,-0.013,6.812,0)"></path></g><g transform="matrix(.013,0,0,-0.013,13.052,0)"></path></g></svg>,</span> and <span><svg height="11.4781pt" style="vertical-align:-3.12928pt" version="1.1" viewbox="-0.0498162 -8.34882 19.464 11.4781" width="19.464pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,0,0)"><use xlink:href="#g121-110"></use></g><g transform="matrix(.013,0,0,-0.013,6.812,0)"><use xlink:href="#g113-55"></use></g><g transform="matrix(.013,0,0,-0.013,13.052,0)"><use xlink:href="#g113-54"></use></g></svg>)</span> in Müller cells or murine retinal tissues. ROS production was detected by CM-H2DCFDA staining, and the concentration of oxidative stress markers (MDA, SOD, and CAT) was estimated by using corresponding commercial kits. Quantification of mRNA expression was conducted by RT-qPCR analysis. The concentration of proinflammatory factors (TNF-<i>α</i>, IL-1<i>β,</i> and IL-6) was evaluated by ELISA. Hematoxylin-eosin staining for murine retinal tissues was performed for histopathological analysis. Immunofluorescence staining was conducted to determine NF-<i>κ</i>B <svg height="11.4781pt" style="vertical-align:-3.12928pt" version="1.1" viewbox="-0.0498162 -8.34882 19.464 11.4781" width="19.464pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,0,0)"><use xlink:href="#g121-110"></use></g><g transform="matrix(.013,0,0,-0.013,6.812,0)"><use xlink:href="#g113-55"></use>
目的。糖尿病视网膜病变(DR)是一种由糖尿病引起的眼部疾病,可导致视力受损甚至失明。氧化应激和炎症是导致 DR 的两个主要致病因素。近来,不同微RNA(miRNA)在DR中的调控作用已得到广泛验证,其中miR-26a-5p已被证实是DR的潜在生物标志物。然而,miR-26a-5p 在 DR 中的具体功能仍不清楚。研究方法利用暴露于高糖(HG)的原代培养小鼠视网膜 Müller 细胞建立体外 DR 模型。通过相差显微镜下的形态观察和谷氨酰胺合成酶的荧光染色来鉴定 Müller 细胞。利用链脲佐菌素诱导的 C57BL/6 糖尿病小鼠构建了 DR 的体内动物模型。用 Western 印迹法对 Müller 细胞的细胞质和线粒体中的细胞色素 c 蛋白水平进行定量,并测量 Müller 细胞或小鼠视网膜组织中胶质纤维酸性蛋白(GFAP)、泛素特异性肽酶 14(USP14)以及与 NF-κB 信号转导相关的因子(p-IκBα、IκBα、p- 和)的蛋白水平。ROS的产生通过CM-H2DCFDA染色检测,氧化应激标记物(MDA、SOD和CAT)的浓度通过相应的商业试剂盒估算。通过 RT-qPCR 分析对 mRNA 表达进行定量。促炎因子(TNF-α、IL-1β 和 IL-6)的浓度通过 ELISA 进行评估。对小鼠视网膜组织进行苏木精-伊红染色,以进行组织病理学分析。通过免疫荧光染色确定了 Müller 细胞中 NF-κB 的核转位。此外,还通过荧光素酶报告实验验证了 miR-26a-5p 与 USP14 之间的相互作用。结果在HG条件下,miR-26a-5p在Müller细胞中下调,而过表达miR-26a-5p可缓解HG诱导的Müller细胞功能障碍。此外,miR-26a-5p靶向USP14,并反向调节USP14的表达。此外,USP14 的敲除或 miR-26a-5p 的上调抑制了 HG 诱导的 NF-κB 信号的激活。拯救实验表明,miR-26a-5p 上调对 HG 诱导的 Müller 细胞功能障碍的保护作用会因 USP14 的过表达而逆转。此外,动物实验还检测到 USP14 在 DR 小鼠视网膜中的上调和 NF-κB 信号的激活。注射 miR-26a-5p 激动剂可改善 DR 小鼠视网膜组织病理学损伤,并降低促炎细胞因子和氧化应激标记物在视网膜中的浓度。结论:miR-26a-5p 通过靶向 USP14 和使 NF-κB 信号通路失活,抑制 DR 进展过程中的氧化应激和炎症反应。
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引用次数: 0
Accommodation and Binocular Vision in Children with Myopic Anisometropia 近视性斜视儿童的屈光度和双眼视力
IF 1.9 4区 医学 Q3 OPHTHALMOLOGY Pub Date : 2024-01-16 DOI: 10.1155/2024/6525136
Chu-chu Zhuang, Ling Zhang, Shan-shan Pan, Yi-ning Wang, Jian-xin Guo
<i>Purpose</i>. To assess the differences in accommodation and binocular vision in children with myopic anisometropia and determine the correlation with anisometropia. <i>Method</i>. A total of 110 patients with myopia aged 8–15 years were recruited from June 2021 to February 2022 from the Affiliated Hospital of Xuzhou Medical University. Based on the interocular differences of spherical equivalent refraction, patients were divided into the isometropia (35 children), low anisometropia (LA group, 42 children), and high anisometropia (HA group, 33 children). The variables assessed were refraction, heterophoria, amplitude of accommodation (AMP), accommodative response (AR), gradient AC/A, positive and negative relative accommodation (PRA/NRA), and near stereopsis in the three groups. Pearson’s correlation coefficient tests were used to investigate the possible association between each parameter and interocular differences (IODs). <i>Results</i>. Among 110 subjects, there were 49 males and 61 females with a mean age of 11.39 ± 2.28 years. Compared with those in the isometropia group, AMP was lower and near stereopsis was higher in the LA group, and the distance and near heterophoria, PRA, AR, and near stereopsis were higher, and PRA, AMP, and gradient AC/A were lower in the HA group (all <span><svg height="9.2729pt" style="vertical-align:-0.6370001pt" version="1.1" viewbox="-0.0498162 -8.6359 19.289 9.2729" width="19.289pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,0,0)"></path></g><g transform="matrix(.013,0,0,-0.013,11.658,0)"></path></g></svg><span></span><span><svg height="9.2729pt" style="vertical-align:-0.6370001pt" version="1.1" viewbox="22.8711838 -8.6359 21.918 9.2729" width="21.918pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,22.921,0)"></path></g><g transform="matrix(.013,0,0,-0.013,29.161,0)"></path></g><g transform="matrix(.013,0,0,-0.013,32.125,0)"><use xlink:href="#g113-49"></use></g><g transform="matrix(.013,0,0,-0.013,38.365,0)"></path></g></svg>).</span></span> Compared with those in the LA group, the near stereopsis, AR, and the near stereopsis were higher in the HA group, and the gradient AC/A was lower (all <span><svg height="9.2729pt" style="vertical-align:-0.6370001pt" version="1.1" viewbox="-0.0498162 -8.6359 19.289 9.2729" width="19.289pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,0,0)"><use xlink:href="#g113-81"></use></g><g transform="matrix(.013,0,0,-0.013,11.658,0)"><use xlink:href="#g117-91"></use></g></svg><span></span><span><svg height="9.2729pt" style="vertical-align:-0.6370001pt" version="1.1" viewbox="22.8711838 -8.6359 21.918 9.2729" width="21.918pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,22.921,0)"><use xlink:href="#g113-49"
目的评估近视性斜视儿童在调节和双眼视力方面的差异,并确定其与斜视的相关性。方法。从 2021 年 6 月至 2022 年 2 月,在徐州医科大学附属医院共招募了 110 名 8-15 岁的近视患者。根据眼球等效屈光度的眼球间差异,将患者分为等视力组(35 名儿童)、低度异视力组(LA 组,42 名儿童)和高度异视力组(HA 组,33 名儿童)。评估的变量包括三组儿童的屈光度、异视角、适应振幅(AMP)、适应反应(AR)、梯度 AC/A、正负相对适应(PRA/NRA)和近立体视。采用皮尔逊相关系数检验来研究各参数与眼间距(IOD)之间可能存在的关联。结果。110 名受试者中有 49 名男性和 61 名女性,平均年龄为 11.39 ± 2.28 岁。与等视组相比,LA组的AMP较低,近立体视较高,而HA组的远近异视、PRA、AR和近立体视较高,PRA、AMP和梯度AC/A较低(均为)。与 LA 组相比,HA 组的近立体视、AR 和近立体视较高,梯度 AC/A 较低(全部)。然而,负相对视差()无明显差异。据观察,远近异视角、AR、AMP 和近立体视分别与 IODs 相关(r = -0.259,;r = -0.201,;r = 0.306,;r = -0.315,;r = 0.535,)。结论我们的研究结果表明,随着近视度数的增加,近视儿童的远近异视、AR、AMP和近立体视有恶化的趋势。
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引用次数: 0
Changes in Corneal Higher-Order Aberrations and Ocular Biometric Measurements after Phacoemulsification Combined with Goniosynechialysis in Primary Angle Closure/Glaucoma Patients 原发性角膜闭合症/青光眼患者接受超声乳化联合球鼻透析术后角膜高阶像差和眼部生物测量的变化
IF 1.9 4区 医学 Q3 OPHTHALMOLOGY Pub Date : 2024-01-12 DOI: 10.1155/2024/5833543
Jiali Xia, Siqi Guo, Fei Hu, Liqi Fan, Ling Yu, Jian Ye
<i>Purpose</i>. To compare corneal higher-order aberrations (HOAs), refractive error, and ocular biological parameters before and after phacoemulsification combined with goniosynechialysis (Phaco-GSL) in primary angle closure/glaucoma (PAC/PACG) patients with different axial lengths (ALs). <i>Methods</i>. In this prospective study, cataract patients diagnosed with PAC/PACG were categorized into two groups based on their ALs: the short AL group (AL ≤ 22.5 mm) and the normal AL group (22.5 < AL ≤ 24.5 mm). The pre- and postsurgery measurements of intraocular pressure (IOP) and best-corrected visual acuity (BCVA) were conducted at 1 day, 1 week, 1 month, 3 months, 6 months, and 12 months. Additionally, the assessments included corneal HOAs, the number of antiglaucoma medications, visual field parameters, manifest refraction, and other ocular biological parameters before surgery and at the final follow-up. <i>Results</i>. Prior to surgery, the two groups exhibited no significant differences, except for AL, curvature value, and <i>Z</i> (4, 0) of the posterior corneal surface (all <span><svg height="9.2729pt" style="vertical-align:-0.6370001pt" version="1.1" viewbox="-0.0498162 -8.6359 19.289 9.2729" width="19.289pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,0,0)"></path></g><g transform="matrix(.013,0,0,-0.013,11.658,0)"></path></g></svg><span></span><span><svg height="9.2729pt" style="vertical-align:-0.6370001pt" version="1.1" viewbox="22.8711838 -8.6359 21.918 9.2729" width="21.918pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,22.921,0)"></path></g><g transform="matrix(.013,0,0,-0.013,29.161,0)"></path></g><g transform="matrix(.013,0,0,-0.013,32.125,0)"><use xlink:href="#g113-49"></use></g><g transform="matrix(.013,0,0,-0.013,38.365,0)"></path></g></svg>).</span></span> Following surgery, BCVA improved, and IOP decreased significantly in both groups (<span><svg height="9.2729pt" style="vertical-align:-0.6370001pt" version="1.1" viewbox="-0.0498162 -8.6359 19.289 9.2729" width="19.289pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,0,0)"><use xlink:href="#g113-81"></use></g><g transform="matrix(.013,0,0,-0.013,11.658,0)"><use xlink:href="#g117-91"></use></g></svg><span></span><span><svg height="9.2729pt" style="vertical-align:-0.6370001pt" version="1.1" viewbox="22.8711838 -8.6359 21.918 9.2729" width="21.918pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,22.921,0)"><use xlink:href="#g113-49"></use></g><g transform="matrix(.013,0,0,-0.013,29.161,0)"><use xlink:href="#g113-47"></use></g><g transform="matrix(.013,0,0,-0.013,32.125,0)"><use xlink:href="#g113-49"></use></g><g transform="matrix(.013,0,0,-0.013,38.365,0)"><use xlink:href="#g113-50"></use></g></svg>).</spa
目的。比较不同轴长(ALs)的原发性闭角型/青光眼(PAC/PACG)患者在接受超声乳化联合声神经透析(Phaco-GSL)前后的角膜高阶像差(HOA)、屈光不正和眼部生物参数。方法。在这项前瞻性研究中,被诊断为 PAC/PACG 的白内障患者根据其轴长分为两组:短轴长组(轴长小于 22.5 毫米)和正常轴长组(22.5 < 轴长小于 24.5 毫米)。手术前后分别在 1 天、1 周、1 个月、3 个月、6 个月和 12 个月测量眼压(IOP)和最佳矫正视力(BCVA)。此外,评估还包括角膜 HOAs、抗青光眼药物的数量、视野参数、屈光表现以及手术前和最终随访时的其他眼部生物参数。结果显示手术前,除角膜厚度、曲率值和角膜后表面 Z(4,0)(均为)外,两组患者无明显差异。手术后,两组的 BCVA 均有所改善,眼压也明显下降()。两组患者的前角膜HOA和全角膜HOA以及Z(3,-3)都有所增加(全部),其中正常角膜AL组的全角膜Z(3,-3)的增加幅度明显高于短角膜AL组()。正常 AL 组还表现出轻微的远视趋势()。两组的视野指数和平均偏差均有显著变化()。结论。Phaco-GSL导致角膜HOAs增加,尤其是三叶形,根据患者的AL而变化。角膜屈光度正常的患者在手术后往往会转向远视。
{"title":"Changes in Corneal Higher-Order Aberrations and Ocular Biometric Measurements after Phacoemulsification Combined with Goniosynechialysis in Primary Angle Closure/Glaucoma Patients","authors":"Jiali Xia, Siqi Guo, Fei Hu, Liqi Fan, Ling Yu, Jian Ye","doi":"10.1155/2024/5833543","DOIUrl":"https://doi.org/10.1155/2024/5833543","url":null,"abstract":"&lt;i&gt;Purpose&lt;/i&gt;. To compare corneal higher-order aberrations (HOAs), refractive error, and ocular biological parameters before and after phacoemulsification combined with goniosynechialysis (Phaco-GSL) in primary angle closure/glaucoma (PAC/PACG) patients with different axial lengths (ALs). &lt;i&gt;Methods&lt;/i&gt;. In this prospective study, cataract patients diagnosed with PAC/PACG were categorized into two groups based on their ALs: the short AL group (AL ≤ 22.5 mm) and the normal AL group (22.5 &lt; AL ≤ 24.5 mm). The pre- and postsurgery measurements of intraocular pressure (IOP) and best-corrected visual acuity (BCVA) were conducted at 1 day, 1 week, 1 month, 3 months, 6 months, and 12 months. Additionally, the assessments included corneal HOAs, the number of antiglaucoma medications, visual field parameters, manifest refraction, and other ocular biological parameters before surgery and at the final follow-up. &lt;i&gt;Results&lt;/i&gt;. Prior to surgery, the two groups exhibited no significant differences, except for AL, curvature value, and &lt;i&gt;Z&lt;/i&gt; (4, 0) of the posterior corneal surface (all &lt;span&gt;&lt;svg height=\"9.2729pt\" style=\"vertical-align:-0.6370001pt\" version=\"1.1\" viewbox=\"-0.0498162 -8.6359 19.289 9.2729\" width=\"19.289pt\" xmlns=\"http://www.w3.org/2000/svg\" xmlns:xlink=\"http://www.w3.org/1999/xlink\"&gt;&lt;g transform=\"matrix(.013,0,0,-0.013,0,0)\"&gt;&lt;/path&gt;&lt;/g&gt;&lt;g transform=\"matrix(.013,0,0,-0.013,11.658,0)\"&gt;&lt;/path&gt;&lt;/g&gt;&lt;/svg&gt;&lt;span&gt;&lt;/span&gt;&lt;span&gt;&lt;svg height=\"9.2729pt\" style=\"vertical-align:-0.6370001pt\" version=\"1.1\" viewbox=\"22.8711838 -8.6359 21.918 9.2729\" width=\"21.918pt\" xmlns=\"http://www.w3.org/2000/svg\" xmlns:xlink=\"http://www.w3.org/1999/xlink\"&gt;&lt;g transform=\"matrix(.013,0,0,-0.013,22.921,0)\"&gt;&lt;/path&gt;&lt;/g&gt;&lt;g transform=\"matrix(.013,0,0,-0.013,29.161,0)\"&gt;&lt;/path&gt;&lt;/g&gt;&lt;g transform=\"matrix(.013,0,0,-0.013,32.125,0)\"&gt;&lt;use xlink:href=\"#g113-49\"&gt;&lt;/use&gt;&lt;/g&gt;&lt;g transform=\"matrix(.013,0,0,-0.013,38.365,0)\"&gt;&lt;/path&gt;&lt;/g&gt;&lt;/svg&gt;).&lt;/span&gt;&lt;/span&gt; Following surgery, BCVA improved, and IOP decreased significantly in both groups (&lt;span&gt;&lt;svg height=\"9.2729pt\" style=\"vertical-align:-0.6370001pt\" version=\"1.1\" viewbox=\"-0.0498162 -8.6359 19.289 9.2729\" width=\"19.289pt\" xmlns=\"http://www.w3.org/2000/svg\" xmlns:xlink=\"http://www.w3.org/1999/xlink\"&gt;&lt;g transform=\"matrix(.013,0,0,-0.013,0,0)\"&gt;&lt;use xlink:href=\"#g113-81\"&gt;&lt;/use&gt;&lt;/g&gt;&lt;g transform=\"matrix(.013,0,0,-0.013,11.658,0)\"&gt;&lt;use xlink:href=\"#g117-91\"&gt;&lt;/use&gt;&lt;/g&gt;&lt;/svg&gt;&lt;span&gt;&lt;/span&gt;&lt;span&gt;&lt;svg height=\"9.2729pt\" style=\"vertical-align:-0.6370001pt\" version=\"1.1\" viewbox=\"22.8711838 -8.6359 21.918 9.2729\" width=\"21.918pt\" xmlns=\"http://www.w3.org/2000/svg\" xmlns:xlink=\"http://www.w3.org/1999/xlink\"&gt;&lt;g transform=\"matrix(.013,0,0,-0.013,22.921,0)\"&gt;&lt;use xlink:href=\"#g113-49\"&gt;&lt;/use&gt;&lt;/g&gt;&lt;g transform=\"matrix(.013,0,0,-0.013,29.161,0)\"&gt;&lt;use xlink:href=\"#g113-47\"&gt;&lt;/use&gt;&lt;/g&gt;&lt;g transform=\"matrix(.013,0,0,-0.013,32.125,0)\"&gt;&lt;use xlink:href=\"#g113-49\"&gt;&lt;/use&gt;&lt;/g&gt;&lt;g transform=\"matrix(.013,0,0,-0.013,38.365,0)\"&gt;&lt;use xlink:href=\"#g113-50\"&gt;&lt;/use&gt;&lt;/g&gt;&lt;/svg&gt;).&lt;/spa","PeriodicalId":16674,"journal":{"name":"Journal of Ophthalmology","volume":"74 1","pages":""},"PeriodicalIF":1.9,"publicationDate":"2024-01-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139463538","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The Relationship between Complements and Age-Related Macular Degeneration and Its Pathogenesis 补体与老年性黄斑变性的关系及其发病机制
IF 1.9 4区 医学 Q3 OPHTHALMOLOGY Pub Date : 2024-01-02 DOI: 10.1155/2024/6416773
Liyuan Chu, Chaoran Bi, Caiming Wang, Hongyan Zhou
Age-related macular degeneration is a retinal disease that causes permanent loss of central vision in people over the age of 65. Its pathogenesis may be related to mitochondrial dysfunction, inflammation, apoptosis, autophagy, complement, intestinal flora, and lipid disorders. In addition, the patient’s genes, age, gender, cardiovascular disease, unhealthy diet, and living habits may also be risk factors for this disease. Complement proteins are widely distributed in serum and tissue fluid. In the early 21st century, a connection was found between the complement cascade and age-related macular degeneration. However, little is known about the effect of complement factors on the pathogenesis of age-related macular degeneration. This article reviews the factors associated with age-related macular degeneration, the relationship between each factor and complement, the related functions, and variants and provides new ideas for the treatment of this disease.
老年黄斑变性是一种视网膜疾病,会导致 65 岁以上的人永久丧失中心视力。其发病机制可能与线粒体功能障碍、炎症、细胞凋亡、自噬、补体、肠道菌群和脂质紊乱有关。此外,患者的基因、年龄、性别、心血管疾病、不健康的饮食和生活习惯也可能是该病的危险因素。补体蛋白广泛分布于血清和组织液中。21 世纪初,人们发现补体级联与老年性黄斑变性之间存在联系。然而,人们对补体因子对老年性黄斑变性发病机制的影响知之甚少。本文回顾了与老年性黄斑变性相关的因素、各因素与补体之间的关系、相关功能和变异,并为治疗这种疾病提供了新思路。
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引用次数: 0
Corneal Epithelial Thickness Mapping: A Major Review 角膜上皮厚度绘图:主要综述
IF 1.9 4区 医学 Q3 OPHTHALMOLOGY Pub Date : 2024-01-02 DOI: 10.1155/2024/6674747
Mohammad-Ali Abtahi, Amir Hushang Beheshtnejad, Golshan Latifi, Marjan Akbari-Kamrani, Sadegh Ghafarian, Ahmad Masoomi, Seyed Ali Sonbolastan, Hamidreaza Jahanbani-Ardakani, Mehrnaz Atighechian, Laleh Banan, Hosein Nouri, Seyed-Hossein Abtahi
The corneal epithelium (CE) is the outermost layer of the cornea with constant turnover, relative stability, remarkable plasticity, and compensatory properties to mask alterations in the underlying stroma. The advent of quantitative imaging modalities capable of producing epithelial thickness mapping (ETM) has made it possible to characterize better the different patterns of epithelial remodeling. In this comprehensive synthesis, we reviewed all available data on ETM with different methods, including very high-frequency ultrasound (VHF-US) and spectral-domain optical coherence tomography (SD-OCT) in normal individuals, corneal or systemic diseases, and corneal surgical scenarios. We excluded OCT studies that manually measured the corneal epithelial thickness (CET) (e.g., by digital calipers) or the CE (e.g., by confocal scanning or handheld pachymeters). A comparison of different CET measuring technologies and devices capable of producing thickness maps is provided. Normative data on CET and the possible effects of gender, aging, diurnal changes, refraction, and intraocular pressure are discussed. We also reviewed ETM data in several corneal disorders, including keratoconus, corneal dystrophies, recurrent epithelial erosion, herpes keratitis, keratoplasty, bullous keratopathy, carcinoma in situ, pterygium, and limbal stem cell deficiency. The available data on the potential role of ETM in indicating refractive surgeries, planning the procedure, and assessing postoperative changes are reviewed. Alterations in ETM in systemic and ocular conditions such as eyelid abnormalities and dry eye disease and the effects of contact lenses, topical medications, and cataract surgery on the ETM profile are discussed.
角膜上皮(CE)是角膜的最外层,具有不断更替、相对稳定、显著的可塑性以及掩盖下层基质变化的代偿特性。能够绘制上皮厚度图(ETM)的定量成像模式的出现,使得更好地描述上皮重塑的不同模式成为可能。在这份综合综述中,我们回顾了所有可用的 ETM 数据,包括在正常人、角膜或全身性疾病以及角膜手术情况下使用不同方法(包括甚高频超声波 (VHF-US) 和光谱域光学相干断层扫描 (SD-OCT))绘制的 ETM 图。我们排除了手动测量角膜上皮厚度(CET)(如使用数字卡尺)或角膜上皮厚度(CE)(如使用共焦扫描或手持式角膜测厚仪)的 OCT 研究。本报告对不同的 CET 测量技术和能够生成厚度图的设备进行了比较。我们还讨论了 CET 的标准数据以及性别、年龄、昼夜变化、屈光度和眼压可能造成的影响。我们还回顾了几种角膜疾病的 ETM 数据,包括角膜炎、角膜营养不良、复发性上皮糜烂、疱疹性角膜炎、角膜成形术、大泡性角膜病、原位癌、翼状胬肉和角膜缘干细胞缺乏症。本文回顾了 ETM 在指示屈光手术、计划手术和评估术后变化方面的潜在作用的现有数据。还讨论了眼睑异常和干眼症等全身性和眼部疾病对 ETM 的影响,以及隐形眼镜、局部用药和白内障手术对 ETM 的影响。
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引用次数: 0
Modulation of Hypoxia-Inducible Factors and Vascular Endothelial Growth Factor Expressions by Superfood Camu-Camu (Myrciaria dubia) Treatment in ARPE-19 and Fetal Human RPE Cells 超级食品金粟(Myrciaria dubia)对 ARPE-19 和胎儿人类 RPE 细胞中缺氧诱导因子和血管内皮生长因子表达的调节作用
IF 1.9 4区 医学 Q3 OPHTHALMOLOGY Pub Date : 2023-12-30 DOI: 10.1155/2023/6617981
Ayaka Nakai, Deokho Lee, Chiho Shoda, Kazuno Negishi, Hiroyuki Nakashizuka, Satoru Yamagami, Toshihide Kurihara
Background. Anti-vascular endothelial growth factor (anti-VEGF) therapy via intravitreal injection is an effective treatment for patients with abnormal ocular neovascularization, such as age-related macular degeneration (AMD) and diabetic macular edema (DME). However, prolonged and frequent anti-VEGF treatment is associated with a risk of local and systemic adverse events, including geographic atrophy, cerebrovascular disease, and death. Furthermore, some patients do not adequately respond to anti-VEGF therapy. Hypoxia-inducible factor (HIF) is a transcription factor that controls the expression of hypoxia-responsive genes involved in angiogenesis, inflammation, and metabolism. The HIF/VEGF pathway plays an important role in neovascularization, and the inhibition of HIF activation could be an effective biomolecular target for neovascular diseases. The demand for disease prevention or treatment using functional foods such as superfoods has increased in recent years. Few reports to date have focused on the antineovascular effects of superfoods in the retinal pigment epithelium (RPE). In light of the growing demand for functional foods, we aimed to find novel HIF inhibitors from superfoods worked in RPE cells, which could be an adjuvant for anti-VEGF therapy. Methods. Seven superfoods were examined to identify novel HIF inhibitor candidates using luciferase assay screening. We used the human RPE cell line ARPE-19 and fetal human RPE (fhRPE) to investigate the biomolecular actions of novel HIF inhibitors using quantitative PCR and western blotting. Results. Under CoCl2-induced pseudohypoxic condition and 1% oxygen hypoxic incubation, camu-camu (Myrciaria dubia) showed HIF inhibitory effects determined by luciferase assays. Camu-camu downregulated HIF-1α and VEGFA mRNA expressions in a concentration-dependent manner. Camu-camu also inhibited HIF-1α protein expressions, and its inhibitory effect was greater than that of vitamin C, which is present at high levels in camu-camu. Conclusion. The camu-camu extract suppressed the activation of HIF and VEGF in RPE cells. This could assist anti-VEGF therapy in patients with abnormal ocular neovascularization.
背景。通过玻璃体内注射抗血管内皮生长因子(anti-VEGF)疗法是治疗眼部异常新生血管(如老年性黄斑变性(AMD)和糖尿病性黄斑水肿(DME))患者的有效方法。然而,长期频繁的抗血管内皮生长因子治疗与局部和全身不良事件的风险相关,包括地理萎缩、脑血管疾病和死亡。此外,有些患者对抗血管内皮生长因子治疗没有充分反应。缺氧诱导因子(HIF)是一种转录因子,可控制参与血管生成、炎症和新陈代谢的缺氧反应基因的表达。HIF/VEGF 通路在新生血管形成中起着重要作用,抑制 HIF 的活化可成为治疗新生血管疾病的有效生物分子靶点。近年来,人们对利用超级食品等功能食品预防或治疗疾病的需求不断增加。迄今为止,很少有报道关注超级食品对视网膜色素上皮(RPE)的抗血管作用。鉴于人们对功能性食品的需求日益增长,我们旨在从超级食品中寻找可在 RPE 细胞中发挥作用的新型 HIF 抑制剂,这可作为抗血管内皮生长因子疗法的辅助手段。方法。我们研究了七种超级食品,通过荧光素酶测定筛选出新型 HIF 抑制剂候选物。我们利用人体 RPE 细胞系 ARPE-19 和胎儿人 RPE(fhRPE),采用定量 PCR 和 Western 印迹法研究新型 HIF 抑制剂的生物分子作用。结果。在CoCl2诱导的假缺氧条件和1%氧气的缺氧培养条件下,通过荧光素酶测定,Camu-camu(Myrciaria dubia)显示出抑制HIF的作用。Camu-camu 以浓度依赖的方式下调了 HIF-1α 和 VEGFA mRNA 的表达。Camu-camu 还能抑制 HIF-1α 蛋白质的表达,其抑制作用大于维生素 C,而维生素 C 在 Camu-camu 中的含量很高。结论茶树提取物能抑制 RPE 细胞中 HIF 和 VEGF 的活化。这有助于眼部异常新生血管患者的抗血管内皮生长因子治疗。
{"title":"Modulation of Hypoxia-Inducible Factors and Vascular Endothelial Growth Factor Expressions by Superfood Camu-Camu (Myrciaria dubia) Treatment in ARPE-19 and Fetal Human RPE Cells","authors":"Ayaka Nakai, Deokho Lee, Chiho Shoda, Kazuno Negishi, Hiroyuki Nakashizuka, Satoru Yamagami, Toshihide Kurihara","doi":"10.1155/2023/6617981","DOIUrl":"https://doi.org/10.1155/2023/6617981","url":null,"abstract":"<i>Background</i>. Anti-vascular endothelial growth factor (anti-VEGF) therapy via intravitreal injection is an effective treatment for patients with abnormal ocular neovascularization, such as age-related macular degeneration (AMD) and diabetic macular edema (DME). However, prolonged and frequent anti-VEGF treatment is associated with a risk of local and systemic adverse events, including geographic atrophy, cerebrovascular disease, and death. Furthermore, some patients do not adequately respond to anti-VEGF therapy. Hypoxia-inducible factor (HIF) is a transcription factor that controls the expression of hypoxia-responsive genes involved in angiogenesis, inflammation, and metabolism. The HIF/VEGF pathway plays an important role in neovascularization, and the inhibition of HIF activation could be an effective biomolecular target for neovascular diseases. The demand for disease prevention or treatment using functional foods such as superfoods has increased in recent years. Few reports to date have focused on the antineovascular effects of superfoods in the retinal pigment epithelium (RPE). In light of the growing demand for functional foods, we aimed to find novel HIF inhibitors from superfoods worked in RPE cells, which could be an adjuvant for anti-VEGF therapy. <i>Methods</i>. Seven superfoods were examined to identify novel HIF inhibitor candidates using luciferase assay screening. We used the human RPE cell line ARPE-19 and fetal human RPE (fhRPE) to investigate the biomolecular actions of novel HIF inhibitors using quantitative PCR and western blotting. <i>Results</i>. Under CoCl<sub>2</sub>-induced pseudohypoxic condition and 1% oxygen hypoxic incubation, camu-camu (<i>Myrciaria dubia</i>) showed HIF inhibitory effects determined by luciferase assays. Camu-camu downregulated <i>HIF-1α</i> and <i>VEGFA</i> mRNA expressions in a concentration-dependent manner. Camu-camu also inhibited HIF-1<i>α</i> protein expressions, and its inhibitory effect was greater than that of vitamin C, which is present at high levels in camu-camu. <i>Conclusion</i>. The camu-camu extract suppressed the activation of HIF and VEGF in RPE cells. This could assist anti-VEGF therapy in patients with abnormal ocular neovascularization.","PeriodicalId":16674,"journal":{"name":"Journal of Ophthalmology","volume":"206 1","pages":""},"PeriodicalIF":1.9,"publicationDate":"2023-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139066374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Different Methods of Secondary Intraocular Lens Implantation 二次眼内透镜植入的不同方法
IF 1.9 4区 医学 Q3 OPHTHALMOLOGY Pub Date : 2023-12-19 DOI: 10.1155/2023/9847067
Matteo Forlini, B. Malyugin, Ike Ahmed, Gabor Scharioth, Rodolfo Mastropasqua, A. Mularoni
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引用次数: 0
Application of ImageJ in Optical Coherence Tomography Angiography (OCT-A): A Literature Review. ImageJ在光学相干断层血管造影(OCT-A)中的应用:文献综述。
IF 1.8 4区 医学 Q3 OPHTHALMOLOGY Pub Date : 2023-11-22 eCollection Date: 2023-01-01 DOI: 10.1155/2023/9479183
Masoud Rahimi, Esmaeil Asadi Khameneh, Hamid Riazi-Esfahani, Tahereh Mahmoudi, Elias Khalili Pour, Rahele Kafieh

Background: This study aimed to review the literature on the application of ImageJ in optical coherence tomography angiography (OCT-A) images.

Methods: A general search was performed in PubMed, Google Scholar, and Scopus databases. The authors evaluated each of the selected articles in order to assess the implementation of ImageJ in OCT-A images.

Results: ImageJ can aid in reducing artifacts, enhancing image quality to increase the accuracy of the process and analysis, processing and analyzing images, generating comparable parameters such as the parameters that assess perfusion of the layers (vessel density (VD), skeletonized density (SD), and vessel length density (VLD)) and the parameters that evaluate the structure of the layers (fractal dimension (FD), vessel density index (VDI), and lacunarity (LAC)), and the foveal avascular zone (FAZ) that are used widely in the retinal and choroidal studies), and establishing diagnostic criteria. It can help to save time when the dataset is huge with numerous plugins and options for image processing and analysis with reliable results. Diverse studies implemented distinct binarization and thresholding techniques, resulting in disparate outcomes and incomparable parameters. Uniformity in methodology is required to acquire comparable data from studies employing diverse processing and analysis techniques that yield varied outcomes.

Conclusion: Researchers and professionals might benefit from using ImageJ because of how quickly and correctly it processes and analyzes images. It is highly adaptable and potent software, allowing users to evaluate images in a variety of ways. There exists a diverse range of methodologies for analyzing OCTA images through the utilization of ImageJ. However, it is imperative to establish a standardized strategy to ensure the reliability and consistency of the method for research purposes.

背景:本研究旨在回顾ImageJ在光学相干断层血管造影(OCT-A)成像中的应用文献。方法:在PubMed、Google Scholar和Scopus数据库中进行常规检索。为了评估ImageJ在OCT-A图像中的实现,作者评估了每一篇选定的文章。结果:ImageJ可以帮助减少伪影,提高图像质量以提高过程和分析的准确性,处理和分析图像,生成可比较的参数,如评估层灌注的参数(血管密度(VD),骨架密度(SD)和血管长度密度(VLD))和评估层结构的参数(分形维数(FD),血管密度指数(VDI)和空隙度(LAC))。以及在视网膜和脉络膜研究中广泛使用的中央凹无血管区(FAZ),并建立诊断标准。当数据集很大时,它可以帮助节省时间,因为有许多插件和选项用于图像处理和分析,并提供可靠的结果。不同的研究采用不同的二值化和阈值化技术,导致不同的结果和不可比较的参数。从采用不同处理和分析技术产生不同结果的研究中获得可比数据需要方法上的一致性。结论:研究人员和专业人士可能会受益于使用ImageJ,因为它处理和分析图像的速度和准确性。它是一款适应性强、功能强大的软件,允许用户以多种方式评估图像。通过使用ImageJ分析OCTA图像有多种方法。然而,为了保证研究方法的可靠性和一致性,必须建立一个标准化的策略。
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引用次数: 0
Gender- and Age-Related Differences of Ocular Biometric Parameters in Patients Undergoing Cataract Surgery in Bosnia and Herzegovina. 波斯尼亚和黑塞哥维那白内障手术患者眼部生物特征参数的性别和年龄相关差异
IF 1.9 4区 医学 Q3 OPHTHALMOLOGY Pub Date : 2023-11-21 eCollection Date: 2023-01-01 DOI: 10.1155/2023/1950257
Edita Zvorničanin, Zoran Vatavuk, Maja Popović, Jasmin Zvorničanin

Purpose: The aim of the study is to determine the distribution and mutual relationship of ocular biometric parameters, as well as to evaluate gender- and age-related differences in patients undergoing cataract surgery in Bosnia and Herzegovina.

Materials and methods: It was a retrospective cross-sectional study of consecutive patients who underwent cataract surgery between January 2017 and December 2021 in a tertiary care clinic. All biometric measurements were performed using the optical biometer OA-2000 (Tomey, Nagoya, Japan).

Results: The study evaluated 1278 eyes from 1278 consecutive cataract patients. The average age of all included patients was 69.4 ± 9.98 (range 40-96). A total of 672 eyes (52.58%) were from females. The mean axial length (AL), anterior chamber depth (ACD), lens thickness (LT), and mean keratometry were 23.46 ± 1.18 mm, 3.17 ± 0.40 mm, 4.54 ± 0.48 mm, and 43.42 ± 1.55D, respectively. Corneal astigmatism of ≥1D, >2D and >3D was found in 33.4%, 7.8% and 2.5% patients, respectively. Females were found to have shorter AL (p < 0.0001), shallower ACD (p < 0.0001) and steeper corneas (p < 0.0001). In both genders, AL, ACD and with the rule astigmatism showed a decreasing trend (p = 0.0001), while keratometry, the average cylinder, and against the rule astigmatism showed an increasing trend (p = 0.0001) with increasing age. Furthermore, in both genders, there was an increasing trend in ACD (p = 0.0001), and a decreasing trend in keratometry (p = 0.0001) and LT (p = 0.0001) with increasing AL.

Conclusions: This study provides useful reference data on ocular biometry for cataract surgeons in Bosnia and Herzegovina. Female patients tend to have steeper corneas, shorter AL and shallower AC than males, and these differences are independent of age or AL.

目的:研究的目的是确定眼部生物特征参数的分布和相互关系,以及评估波斯尼亚和黑塞哥维那白内障手术患者的性别和年龄相关差异。材料和方法:这是一项回顾性横断面研究,纳入了2017年1月至2021年12月在三级保健诊所接受白内障手术的连续患者。所有生物测量均使用OA-2000光学生物计(Tomey,名古屋,日本)进行。结果:本研究对1278例连续白内障患者的1278只眼进行了评估。所有患者的平均年龄为69.4±9.98岁(40 ~ 96岁)。女性672只,占52.58%。平均眼轴长(AL)、前房深度(ACD)、晶状体厚度(LT)、平均角膜度数分别为23.46±1.18 mm、3.17±0.40 mm、4.54±0.48 mm、43.42±1.55D。角膜散光≥1D、>2D和>3D分别占33.4%、7.8%和2.5%。女性AL较短(p p p p = 0.0001),随着年龄的增长,角膜度数、平均柱体、反规则散光呈增加趋势(p = 0.0001)。此外,随着al的增加,男女ACD呈上升趋势(p = 0.0001),角膜度数(p = 0.0001)和LT呈下降趋势(p = 0.0001)。结论:本研究为波黑白内障手术提供了有用的眼生物测量参考数据。女性患者的角膜比男性更陡,AL更短,AC更浅,这些差异与年龄或AL无关。
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引用次数: 0
A Prospective Study of Clinical Features of Anterior Uveitis in Taiwan 台湾地区前葡萄膜炎临床特征的前瞻性研究
4区 医学 Q3 OPHTHALMOLOGY Pub Date : 2023-11-07 DOI: 10.1155/2023/9647418
Wei-Yu Chiang, Shih-Chou Chen, Shwu-Jiuan Sheu, Hsi-Kung Kuo
In this study, we reported the patterns, epidemiology, and clinical features of anterior uveitis (AU) in Taiwan, an area of Eastern Asia. This prospective, cross-sectional case series study was performed to identify patients with AU at two tertiary medical centers (Kaohsiung Chang Gung Memorial Hospital and Kaohsiung Veterans General Hospital) located at the southern Taiwan between December 1, 2018, and March 31, 2020. The clinical diagnoses, ocular presentations, and laboratory data, including the results of the aqueous polymerase chain reaction tests, were investigated in these patients. A total of 112 patients, with a mean age of 48.9 years, were included. Most patients (87.5%) presented with unilateral eye disease, with 30 cases of ocular hypertension at the first presentation (27%). The most common clinical diagnoses were idiopathic AU (37.5%), human leukocyte antigen (HLA)-B27-associated acute AU (25.0%), and herpetic AU (18.8%). Among patients with herpetic AU, cytomegalovirus (CMV) was the most common pathogen (17/21, 81%). Compared to HLA-B27-associated acute AU, CMV-related AU was mostly observed in patients that were older in age, exhibited higher intraocular pressure, more keratic precipitates, greater iris atrophy, and more pseudophakia, but was least reported in those with posterior synechiae. This prospective study identified the pattern and clinical features of AU in southern Taiwan.
本研究报告东亚台湾地区前葡萄膜炎(AU)的型态、流行病学及临床特征。本前瞻性横断面病例系列研究于2018年12月1日至2020年3月31日在位于台湾南部的两家三级医疗中心(高雄长工纪念医院和高雄退伍军人总医院)进行,以确定患有AU的患者。研究了这些患者的临床诊断、眼部表现和实验室数据,包括水聚合酶链反应试验的结果。共纳入112例患者,平均年龄48.9岁。大多数患者(87.5%)表现为单侧眼病,其中30例首次出现高眼压(27%)。最常见的临床诊断为特发性AU(37.5%)、人白细胞抗原(HLA)- b27相关的急性AU(25.0%)和疱疹性AU(18.8%)。在疱疹性AU患者中,巨细胞病毒(CMV)是最常见的病原体(17/21,81%)。与hla - b27相关的急性AU相比,cmv相关的AU主要发生在年龄较大、眼压较高、角膜沉淀较多、虹膜萎缩较大和假性晶状体较多的患者中,但在后粘连患者中报道最少。本前瞻性研究确定了台湾南部AU的模式和临床特征。
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引用次数: 0
期刊
Journal of Ophthalmology
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