Background/Purpose: To describe some complications of the radiofrequency ablation method in patients with non-small cell lung cancer at Nghe An Oncology Hospital. Methods: The non-controlled clinical intervention study, compared the results before and after on 43 non-small cell lung cancer patients at Nghe An Oncology Hospital. Results: 16 complications occurred, accounting for 37.2%. Complications after radiofrequency ablation include hemoptysis, pneumothorax, pneumothorax that must be drained, hemothorax, and pneumonia. The rate of complications in pneumothorax was the highest at 25.6, lower was hemoptysis at 11.6%, followed by post-procedural pneumonia at 4.7% in 1st and 14.3% in 2nd, the remaining complications accounted for a small rate. Conclusion: No statistically significant differences were found in age, sex, RFA frequency, RFA duration, and needle type used with complications after RFA.
{"title":"Some complications of the radiofrequency ablation method in patients with non-small cell lung cancer at Nghe An Oncology Hospital","authors":"Pham Vinh Hung, V. T. Huong, Nguyen Thi Mai Huong","doi":"10.56086/jcvb.v2i3.72","DOIUrl":"https://doi.org/10.56086/jcvb.v2i3.72","url":null,"abstract":"Background/Purpose: To describe some complications of the radiofrequency ablation method in patients with non-small cell lung cancer at Nghe An Oncology Hospital. \u0000Methods: The non-controlled clinical intervention study, compared the results before and after on 43 non-small cell lung cancer patients at Nghe An Oncology Hospital. \u0000Results: 16 complications occurred, accounting for 37.2%. Complications after radiofrequency ablation include hemoptysis, pneumothorax, pneumothorax that must be drained, hemothorax, and pneumonia. The rate of complications in pneumothorax was the highest at 25.6, lower was hemoptysis at 11.6%, followed by post-procedural pneumonia at 4.7% in 1st and 14.3% in 2nd, the remaining complications accounted for a small rate. \u0000Conclusion: No statistically significant differences were found in age, sex, RFA frequency, RFA duration, and needle type used with complications after RFA.","PeriodicalId":166965,"journal":{"name":"JOURNAL OF CONTROL VACCINE AND BIOLOGICALS","volume":"12 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123081694","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
N. T. Kiều, Doan Huu Thien, Le Thi Hai Yen, Nguyễn Thị Thu Hương, Nguyen Thi Tuyet Hoa, Do Thi Phuong
In early 2019, the National Institute for Control of Vaccines and Biologicals (NICVB) implemented a national-level project to establish the first national standard for rotavirus vaccines to be used in the quality control of rotavirus vaccines, code MCQG.Ro.01. However, a stability assessment of the national standard has not been carried out yet. NICVB had performed a study entitled "Stability assessment of the first national standard for rotavirus vaccine" with the purpose of providing the prediction shelf life through a thermally accelerated degradation study. In this study, the first national standard for rotavirus vaccine was stored at 4 °C, 25 °C, and 37 °C. At each temperature, the potency of the vaccine was evaluated after 3 days, 5 days, 7 days and 10 days. The stability data were analysed by JMP Pro13 software, the software used to predict the expiry date of vaccines and biological products based on the Arrhenius equation. As a result, the first national standard for rotavirus vaccine, code MCQG.Ro.01, has a predicted shelf life of 27 years when stored at -70 °C.
{"title":"STABILITY ASSESSMENT OF THE FIRST NATIONAL STANDARD FOR ROTAVIRUS VACCINE","authors":"N. T. Kiều, Doan Huu Thien, Le Thi Hai Yen, Nguyễn Thị Thu Hương, Nguyen Thi Tuyet Hoa, Do Thi Phuong","doi":"10.56086/jcvb.v2i3.67","DOIUrl":"https://doi.org/10.56086/jcvb.v2i3.67","url":null,"abstract":"In early 2019, the National Institute for Control of Vaccines and Biologicals (NICVB) implemented a national-level project to establish the first national standard for rotavirus vaccines to be used in the quality control of rotavirus vaccines, code MCQG.Ro.01. However, a stability assessment of the national standard has not been carried out yet. NICVB had performed a study entitled \"Stability assessment of the first national standard for rotavirus vaccine\" with the purpose of providing the prediction shelf life through a thermally accelerated degradation study. In this study, the first national standard for rotavirus vaccine was stored at 4 °C, 25 °C, and 37 °C. At each temperature, the potency of the vaccine was evaluated after 3 days, 5 days, 7 days and 10 days. The stability data were analysed by JMP Pro13 software, the software used to predict the expiry date of vaccines and biological products based on the Arrhenius equation. As a result, the first national standard for rotavirus vaccine, code MCQG.Ro.01, has a predicted shelf life of 27 years when stored at -70 °C.","PeriodicalId":166965,"journal":{"name":"JOURNAL OF CONTROL VACCINE AND BIOLOGICALS","volume":"81 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124902220","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
N. T. Huong, Nguyen Thi Trinh, P. V. Hung, Nguyen Thi Tu Linh
Background/Purpose: To evaluate cell block (CB) and cell smear (CS) techniques in the diagnostic cytology of body-cavity fluids. Methods: The cross-sectional descriptive study was conducted based on the anatomical results of 106 patients of Viet Tiep Hospital Results: Most of the samples were pleural fluid, accounting for 73.6%, pink fluid was the main color (46.2%), and the amount of fluid taken from each specimen was mostly from 201 - 250 ml (53.8%). Through CB, staining H&E, and immunohistochemistry, we identified 49 cancer cases, most of which were adenocarcinomas accounting for 87.8%. On the cell block technique, all adenocarcinoma samples have large cell nuclei, increased nucleus/cytoplasmic ratio, over 90% have glandular structure, over 80% of chromatin is crude, the nucleus is clear and there is space around the tumor. Conclusion: Comparing the results of cytology between CB and CS techniques shows that CB has a higher diagnostic value.
{"title":"Evaluating cell block and cell smear technique in the body-cavity fluids cytology technique at Viet Tiep Hospital","authors":"N. T. Huong, Nguyen Thi Trinh, P. V. Hung, Nguyen Thi Tu Linh","doi":"10.56086/jcvb.v2i3.70","DOIUrl":"https://doi.org/10.56086/jcvb.v2i3.70","url":null,"abstract":"Background/Purpose: To evaluate cell block (CB) and cell smear (CS) techniques in the diagnostic cytology of body-cavity fluids. \u0000Methods: The cross-sectional descriptive study was conducted based on the anatomical results of 106 patients of Viet Tiep Hospital \u0000Results: Most of the samples were pleural fluid, accounting for 73.6%, pink fluid was the main color (46.2%), and the amount of fluid taken from each specimen was mostly from 201 - 250 ml (53.8%). Through CB, staining H&E, and immunohistochemistry, we identified 49 cancer cases, most of which were adenocarcinomas accounting for 87.8%. On the cell block technique, all adenocarcinoma samples have large cell nuclei, increased nucleus/cytoplasmic ratio, over 90% have glandular structure, over 80% of chromatin is crude, the nucleus is clear and there is space around the tumor. \u0000Conclusion: Comparing the results of cytology between CB and CS techniques shows that CB has a higher diagnostic value.","PeriodicalId":166965,"journal":{"name":"JOURNAL OF CONTROL VACCINE AND BIOLOGICALS","volume":"32 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128134594","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Luu Thi Dung, N. T. Ly, Nguyen Thi Hong Dinh, P. Hung, N. Tung, Doan Huu Thien
Based on Bovine serum albumin (BSA) is commonly used as a culture medium of vaccine production. According to the World Health Organization, residual BSA with high concentrations in vaccines has an adverse effect on human health. Therefore, the research team evaluated the technical parameters including accuracy, accuracy and linearity of the procedure for determining BSA content by ELISA method in vaccines. The residual BSA content in the experimental vaccine (Varivax) was carried out according to the procedure of the BSA diagnostic kit, China by the Elisa method. The sample vaccine is diluted in 3 dilutions: 1/20, 1/30, 1/40 so that the OD value of the sample vaccine is within the range of OD values at BSA calibration curve concentrations from 2, 5ng/ml - 40 ng/ml. Results of study showed that the process specifications were determined including accuracy (t-table (- 2.29 - 2.39) < tα = 2.571), precision (%CV=0.79-1, 21%), linearity (R2 = 0.995, %CV = 0.28 - 4.47%, Δi=-2.47 - 10.95%). We conclussion that the process of determining BSA content by the Elisa method was evaluated as satisfactory with the specifications of accuracy, precision and linearity.
{"title":"DETERMINATE ACCURACY AND LINEARITY OF BSA TEST IN VACCINE BY ELISA","authors":"Luu Thi Dung, N. T. Ly, Nguyen Thi Hong Dinh, P. Hung, N. Tung, Doan Huu Thien","doi":"10.56086/jcvb.v2i3.64","DOIUrl":"https://doi.org/10.56086/jcvb.v2i3.64","url":null,"abstract":"Based on Bovine serum albumin (BSA) is commonly used as a culture medium of vaccine production. According to the World Health Organization, residual BSA with high concentrations in vaccines has an adverse effect on human health. Therefore, the research team evaluated the technical parameters including accuracy, accuracy and linearity of the procedure for determining BSA content by ELISA method in vaccines. \u0000The residual BSA content in the experimental vaccine (Varivax) was carried out according to the procedure of the BSA diagnostic kit, China by the Elisa method. The sample vaccine is diluted in 3 dilutions: 1/20, 1/30, 1/40 so that the OD value of the sample vaccine is within the range of OD values at BSA calibration curve concentrations from 2, 5ng/ml - 40 ng/ml. \u0000Results of study showed that the process specifications were determined including accuracy (t-table (- 2.29 - 2.39) < tα = 2.571), precision (%CV=0.79-1, 21%), linearity (R2 = 0.995, %CV = 0.28 - 4.47%, Δi=-2.47 - 10.95%). \u0000We conclussion that the process of determining BSA content by the Elisa method was evaluated as satisfactory with the specifications of accuracy, precision and linearity.","PeriodicalId":166965,"journal":{"name":"JOURNAL OF CONTROL VACCINE AND BIOLOGICALS","volume":"88 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128629403","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
P. Thuy, V. T. Hương, Phạm Thị Minh, Pham Trung Duc, Phung Dang Thi Ngoc
Introduction/ Objectives: According to WHO, clinical samples collected in retrospective clinical studies are required to provide stability claims for each storage condition, such as duration at different temperatures and freeze/thaw cycles[1]. This study was conducted to evaluate the effects of multiple freeze/thaw (FT) cycles on the concentration of RNA SARS-CoV-2 (extracted from retrospective clinical sample) by using Real-time PCR method. Methods: Descriptive laboratory study. We used a commercial Real-time PCR assay and reference standard for quantification of RNA concentration after each FT cycle. Results: We observed that the extracted RNA SARS-CoV-2 remained stable and did not show significantly different Ct values (equivalent to viral loads) over 10 FT cycles (p >0.05), compared to those Ct values at time point 0. Variations of Ct values over 10 FT cycles ranged from 0.64% to 1.64%. The mean difference in viral load was 0,12log10IU/ml. Conclusions: Our study results proved that RNA SARS-CoV-2 extracted from clinical samples can remain stable over 10 freeze-thaw cycles and can be used as a evaluation panel for quality control of in vitro diagnostic test kits detecting SARS-CoV-2.
{"title":"EVALUATION OF THE STABILITY OF RNA SARS-CoV-2 IN MULTIPLE FREZEE-THAW CYCLES USING REAL-TIME PCR METHOD","authors":"P. Thuy, V. T. Hương, Phạm Thị Minh, Pham Trung Duc, Phung Dang Thi Ngoc","doi":"10.56086/jcvb.v2i3.62","DOIUrl":"https://doi.org/10.56086/jcvb.v2i3.62","url":null,"abstract":"Introduction/ Objectives: According to WHO, clinical samples collected in retrospective clinical studies are required to provide stability claims for each storage condition, such as duration at different temperatures and freeze/thaw cycles[1]. This study was conducted to evaluate the effects of multiple freeze/thaw (FT) cycles on the concentration of RNA SARS-CoV-2 (extracted from retrospective clinical sample) by using Real-time PCR method. \u0000Methods: Descriptive laboratory study. We used a commercial Real-time PCR assay and reference standard for quantification of RNA concentration after each FT cycle. \u0000Results: We observed that the extracted RNA SARS-CoV-2 remained stable and did not show significantly different Ct values (equivalent to viral loads) over 10 FT cycles (p >0.05), compared to those Ct values at time point 0. Variations of Ct values over 10 FT cycles ranged from 0.64% to 1.64%. The mean difference in viral load was 0,12log10IU/ml. \u0000Conclusions: Our study results proved that RNA SARS-CoV-2 extracted from clinical samples can remain stable over 10 freeze-thaw cycles and can be used as a evaluation panel for quality control of in vitro diagnostic test kits detecting SARS-CoV-2.","PeriodicalId":166965,"journal":{"name":"JOURNAL OF CONTROL VACCINE AND BIOLOGICALS","volume":"10 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129751344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Application on the Realtime RT-PCR assay is a method to determine the presence of viruses through the detection of genetic material of the SARS-CoV-2 virus, which is a highly accurate method. This study was conducted to validate the Realtime RT-PCR assay for the detection of E gene of SARS-CoV-2 at National Institute for Control of Vaccines and Biologicals (NICVB). Descriptive study in the laboratory. We determined limit of detection (LOD), reproducibility and analytical specificity of the Realtime RT-PCR assay for the detection of E gene of SARS-CoV-2 according WHO guidelines. The study results included as follow the limit of detection (LOD) reached LOD 95 is 5.2 copies/reaction (95%CI 3.3- 8.0); The accuracy with mean CV (%) of repeatability reached 2.00% and reproducibility reached 1.74% and analytical specificity reached 100%. The results all met the approval criteria and comparable to published data by WHO group. Based on results we successful application of Realtime RT-PCR assay for the detection of E gene of SARS-CoV-2 at National Institute for Control of Vaccines and Biologicals
{"title":"Application of Realtime RT-PCR assay for the detection of E gene of SARS-CoV-2","authors":"Dinh Thi Phuong Thao, V. T. Hương, P. Thuy","doi":"10.56086/jcvb.v2i3.68","DOIUrl":"https://doi.org/10.56086/jcvb.v2i3.68","url":null,"abstract":"Application on the Realtime RT-PCR assay is a method to determine the presence of viruses through the detection of genetic material of the SARS-CoV-2 virus, which is a highly accurate method. This study was conducted to validate the Realtime RT-PCR assay for the detection of E gene of SARS-CoV-2 at National Institute for Control of Vaccines and Biologicals (NICVB). \u0000Descriptive study in the laboratory. We determined limit of detection (LOD), reproducibility and analytical specificity of the Realtime RT-PCR assay for the detection of E gene of SARS-CoV-2 according WHO guidelines. \u0000The study results included as follow the limit of detection (LOD) reached LOD 95 is 5.2 copies/reaction (95%CI 3.3- 8.0); The accuracy with mean CV (%) of repeatability reached 2.00% and reproducibility reached 1.74% and analytical specificity reached 100%. The results all met the approval criteria and comparable to published data by WHO group. \u0000Based on results we successful application of Realtime RT-PCR assay for the detection of E gene of SARS-CoV-2 at National Institute for Control of Vaccines and Biologicals","PeriodicalId":166965,"journal":{"name":"JOURNAL OF CONTROL VACCINE AND BIOLOGICALS","volume":"18 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127661943","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Nguyen Thi Trinh, N. T. Huong, Tran Hong Tram, N. T. Kiều
Background/Purpose: To contrast the results of cell block diagnosis with histopathological results and describe some factors that affect the quality of cell blocks. Methods: The cross-sectional descriptive study was conducted based on the anatomical results of 106 patients of Viet Tiep Hospital Results: The cell block technique had a sensitivity of 84.4% significantly higher than the sensitivity of the cell smear of 57.8%. The specificity of both tests is above 80%. Cellblock test results were less accurate in yellow fluid samples (13.3%). Blood fluid and pink fluid samples both have over 90% accuracy. The difference was statistically significant with p<0.05. Cellblock test results were less accurate in samples with a sample count of ≤150 ml (52.9%). Samples with a greater number of specimens were over 80% accurate. The difference was statistically significant with p<0.05. There was no difference in the accuracy of cell block test results between specimens at different sites, cancer types, cancer origins, age groups, and sex of the subjects studied. Conclusion: The cell block technique improves the number of malignant diagnoses compared to histopathology, and has higher sensitivity and lower specificity than the cell smear technique. Factors related to the accuracy of cell block test results include the color of the test fluid and the number of fluids. There was no difference in the accuracy of CB test results on different specimens, different cancer types, different cancer origins, different ages, and genders.
{"title":"Comparing cell block diagnostic results with histopathological results and some factors affecting the quality of cell blocks in the diagnosis of body cavity fluid cells at Viet Tiep Hospital","authors":"Nguyen Thi Trinh, N. T. Huong, Tran Hong Tram, N. T. Kiều","doi":"10.56086/jcvb.v2i3.71","DOIUrl":"https://doi.org/10.56086/jcvb.v2i3.71","url":null,"abstract":"Background/Purpose: To contrast the results of cell block diagnosis with histopathological results and describe some factors that affect the quality of cell blocks. \u0000Methods: The cross-sectional descriptive study was conducted based on the anatomical results of 106 patients of Viet Tiep Hospital \u0000Results: The cell block technique had a sensitivity of 84.4% significantly higher than the sensitivity of the cell smear of 57.8%. The specificity of both tests is above 80%. Cellblock test results were less accurate in yellow fluid samples (13.3%). Blood fluid and pink fluid samples both have over 90% accuracy. The difference was statistically significant with p<0.05. Cellblock test results were less accurate in samples with a sample count of ≤150 ml (52.9%). Samples with a greater number of specimens were over 80% accurate. The difference was statistically significant with p<0.05. There was no difference in the accuracy of cell block test results between specimens at different sites, cancer types, cancer origins, age groups, and sex of the subjects studied. \u0000Conclusion: The cell block technique improves the number of malignant diagnoses compared to histopathology, and has higher sensitivity and lower specificity than the cell smear technique. Factors related to the accuracy of cell block test results include the color of the test fluid and the number of fluids. There was no difference in the accuracy of CB test results on different specimens, different cancer types, different cancer origins, different ages, and genders.","PeriodicalId":166965,"journal":{"name":"JOURNAL OF CONTROL VACCINE AND BIOLOGICALS","volume":"21 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121481527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Nguyen Viet Anh, D. H. Anh, N. T. Ha, T. Phuong, N. T. Ly
Implementation of potency test for new generation Japanese Encephalitis vaccine (IMOJEV) is one of the functions of National Institute for Vaccines and Biologicals (NICVB) prior to market release. Potency test of IMOJEV was carried out by using Plaque Forming Unit (PFU) method for both standard and testing samples, the results was in the approved range of manufacturer. The process of validation of potency test for Japanese Encephalitis vaccine IMOJEV was built and implemented by practical validation method with following parameters: accuracy, repeatability, and intermediate precision of the potency method for Japanese encephalitis vaccine Results of study showed that the parameters of accuracy, repeatability and intermediate precision of potency test for Japanese Encephalitis IMOJEV were all qualified and in the approved range of manufacturer: titer of standard sample was about 4.25-5.09 log PFU/0.5 ml and titer of IMOJEV was between 4.0-5.8 log PFU/0.5ml Based on our study conclussion the the potency test for Japanese Encephalitis IMOJEV after validation was qualified for accuracy, repeatability and intermediate precision with coefficient of variation CV ≤ 25% so it is suitable with laboratory condition of NICVB.
{"title":"VALIDATION OF POTENCY TEST OF NEW GENERATION JAPANESE ENCEPHALITIS VACCINE IMOJEV BY PLAQUE FORMING UNIT (PFU) METHOD","authors":"Nguyen Viet Anh, D. H. Anh, N. T. Ha, T. Phuong, N. T. Ly","doi":"10.56086/jcvb.v2i3.66","DOIUrl":"https://doi.org/10.56086/jcvb.v2i3.66","url":null,"abstract":"Implementation of potency test for new generation Japanese Encephalitis vaccine (IMOJEV) is one of the functions of National Institute for Vaccines and Biologicals (NICVB) prior to market release. Potency test of IMOJEV was carried out by using Plaque Forming Unit (PFU) method for both standard and testing samples, the results was in the approved range of manufacturer. The process of validation of potency test for Japanese Encephalitis vaccine IMOJEV was built and implemented by practical validation method with following parameters: accuracy, repeatability, and intermediate precision of the potency method for Japanese encephalitis vaccine \u0000Results of study showed that the parameters of accuracy, repeatability and intermediate precision of potency test for Japanese Encephalitis IMOJEV were all qualified and in the approved range of manufacturer: titer of standard sample was about 4.25-5.09 log PFU/0.5 ml and titer of IMOJEV was between 4.0-5.8 log PFU/0.5ml \u0000Based on our study conclussion the the potency test for Japanese Encephalitis IMOJEV after validation was qualified for accuracy, repeatability and intermediate precision with coefficient of variation CV ≤ 25% so it is suitable with laboratory condition of NICVB.","PeriodicalId":166965,"journal":{"name":"JOURNAL OF CONTROL VACCINE AND BIOLOGICALS","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131251920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Nguyen Duy Thai, Tran Hong Tram, P. Hung, Doan Huu Thien
The role of sleep deprivation in affecting on social behavior like aggression is not yet fully understood. This study shows how interactions between social factors affect on sleep and the aggressive behavior of adolescents. Data collected from 8,115 survey participants were analyzed by the linear regression model and the mediation model to find links between social factors, sleep deprivation and aggressive behavior by controlling key variables. The results of study show a significant increase in the aggressive behavior of young people who lack of sleep, abuse on-screen time, and are influenced by other factors like age, gender and family. Conclusions: The results demonstrate that on-screen time is an important factor that affects aggressive behavior through sleep deprivation.
{"title":"EXPLORING THE EFFECT OF SLEEP DEPRIVATION AND SOCIAL LIFE ON AGGRESSIVE BEHAVIOR AMONG ADOLESCENTS","authors":"Nguyen Duy Thai, Tran Hong Tram, P. Hung, Doan Huu Thien","doi":"10.56086/jcvb.v2i3.69","DOIUrl":"https://doi.org/10.56086/jcvb.v2i3.69","url":null,"abstract":"The role of sleep deprivation in affecting on social behavior like aggression is not yet fully understood. This study shows how interactions between social factors affect on sleep and the aggressive behavior of adolescents. \u0000Data collected from 8,115 survey participants were analyzed by the linear regression model and the mediation model to find links between social factors, sleep deprivation and aggressive behavior by controlling key variables. \u0000The results of study show a significant increase in the aggressive behavior of young people who lack of sleep, abuse on-screen time, and are influenced by other factors like age, gender and family. Conclusions: The results demonstrate that on-screen time is an important factor that affects aggressive behavior through sleep deprivation.","PeriodicalId":166965,"journal":{"name":"JOURNAL OF CONTROL VACCINE AND BIOLOGICALS","volume":"24 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123587635","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Andrographis paniculata is a traditional medicinal plant which has been known for various beneficial properties, such as the inhibition of bacterial growth and health enhancement properties. This study was carried out to evaluate the antibacterial effects, polyphenol contents and antioxidant activities of extracts from Andrographis paniculata plant materials, so as to partly explain its traditional application. Plant materials were extracted with 6 solvents: hot water, ethanol, methanol, ethyl acetate, acetone and hexane. We applied the agar-well-diffusion method to evaluate the inhibitory effects of extracts on several bacteria strains, including: Bacillus subtilis ATCC 6633; Geobacillus stearothermophilus ATCC 7953; Pseudomonas aeruginosa ATCC 9027; Staphylococcus aureus ATCC 25023; Staphylococcus aureus ATCC 25923; Escherichia coli ATCC 85922; Escherichia coli ATCC 35218; Escherichia coli ATCC 25922 and Salmonella ATCC 13311. Reagents, such as Folin Ciocalteu and 1,1-diphenyl-2-picrylhydrazyl (DPPH), were used to measure the polyphenol contents and antioxidant activities of the extracts, which were then respectedly converted to mg acid chlorogenic equivelent and mg VTME equivelent per 100 mg plant powder. The results showed that extracts from Andrographis paniculata showed antibacterial effects, in which the ethanol extract showed the highest antibacterial effects on bacteria, as it induced inhibitory zones on all testes bacteria, with the largest one was on Escherichia coli ATCC 25922 (18.81±2.22 mm). Extracts with high polyphenol contents and antioxidant activities were methanol, hot water and ethanol, in which the highest polyphenol content belonged to methanol extract (with the value reached to 0.270±0.019 mg acid chlorogenic equivalent per 100 mg plant powder), and the strongest antioxidant activity belong to hot water extract (with the value reached to 0.691±0.115 mg VTME equivalent per 100 mg plant powder).
{"title":"A STUDY TO INVESTIGATE THE ANTIBACTERIAL EFFECTS, POLYPHENOL CONTENTS AND ANTIOXIDANT ACTIVITIES OF EXTRACTS FROM ANDROGRAPHIS PANICULATA MEDICINAL PLANTS","authors":"T. Hong, P. Huế, N. T. T. Ha, Nguyễn Thanh Hải","doi":"10.56086/jcvb.v2i3.65","DOIUrl":"https://doi.org/10.56086/jcvb.v2i3.65","url":null,"abstract":"Andrographis paniculata is a traditional medicinal plant which has been known for various beneficial properties, such as the inhibition of bacterial growth and health enhancement properties. This study was carried out to evaluate the antibacterial effects, polyphenol contents and antioxidant activities of extracts from Andrographis paniculata plant materials, so as to partly explain its traditional application. Plant materials were extracted with 6 solvents: hot water, ethanol, methanol, ethyl acetate, acetone and hexane. We applied the agar-well-diffusion method to evaluate the inhibitory effects of extracts on several bacteria strains, including: Bacillus subtilis ATCC 6633; Geobacillus stearothermophilus ATCC 7953; Pseudomonas aeruginosa ATCC 9027; Staphylococcus aureus ATCC 25023; Staphylococcus aureus ATCC 25923; Escherichia coli ATCC 85922; Escherichia coli ATCC 35218; Escherichia coli ATCC 25922 and Salmonella ATCC 13311. Reagents, such as Folin Ciocalteu and 1,1-diphenyl-2-picrylhydrazyl (DPPH), were used to measure the polyphenol contents and antioxidant activities of the extracts, which were then respectedly converted to mg acid chlorogenic equivelent and mg VTME equivelent per 100 mg plant powder. The results showed that extracts from Andrographis paniculata showed antibacterial effects, in which the ethanol extract showed the highest antibacterial effects on bacteria, as it induced inhibitory zones on all testes bacteria, with the largest one was on Escherichia coli ATCC 25922 (18.81±2.22 mm). Extracts with high polyphenol contents and antioxidant activities were methanol, hot water and ethanol, in which the highest polyphenol content belonged to methanol extract (with the value reached to 0.270±0.019 mg acid chlorogenic equivalent per 100 mg plant powder), and the strongest antioxidant activity belong to hot water extract (with the value reached to 0.691±0.115 mg VTME equivalent per 100 mg plant powder).","PeriodicalId":166965,"journal":{"name":"JOURNAL OF CONTROL VACCINE AND BIOLOGICALS","volume":"290 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132636522","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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