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Impact of Hydrochloric acid on breaking seed dormancy and germination enhancement in native Alhagi graecorum Boiss ( Al-Agool ). 盐酸对本地Alhagi graecorum Boiss (Al-Agool)打破种子休眠和促进发芽的影响。
Pub Date : 2023-08-07 DOI: 10.51984/jopas.v22i2.2496
O. Sharashy
Despite the great importance of legumes plants, most of them have a hard and impermeable testa, which causes physical dormancy of the seeds and prevents germination even when environmental conditions are favorable. This is considered one of the major problems in successful seed germination.  Alhagi graecorum plant is a wild  native legume. It has a hard and impermeable testa, which causes physical dormancy of seeds, and to overcome hard coat imposed dormancy, softening  the  hard seed coat by soaking Hydrochloric acid ( HCl ). The present study was conducted to evaluate various duration of soaking seeds in HCL to breaking physical dormancy for           A.graecorum in 10, 20, and 30 minutes. The results  showed that, final germination percentage (FGP) in treated seeds significantly increased when compared with the control were 55, 62 and  68 % respectively, while in control was only 12 %. Seed germination influenced by HCL is due to its capability to break the hard seed coat that leads to water absorption and  imbibition of seeds. As for the mean daily germination ( MDG ), the results revealed that, there were significance increased in seventh day of sowing in germination between the treated seeds in duration of soaking 20 and 30 min were 23 and 24 % respectively, as they were most effective in germination, while the germination in the control  stopped in the fifth day of sowing at 12 %. While the mean germination time (MGT), the results indicated that the seeds soaked in acid recorded 9.66, 9.13, and 8.62 days, respectively. The minimum MGT for treated seeds was detected in 30 min., 8.62 days. The reason for the long period of the mean germination time may be due to the use of a low concentration of hydrochloric acid, so I recommend using higher concentrations of acid to obtain a lower mean germination time.
尽管豆科植物非常重要,但它们大多数都有坚硬且不透水的皮,这导致种子处于物理休眠状态,即使在环境条件有利的情况下也无法发芽。这被认为是种子成功发芽的主要问题之一。扁豆是一种野生豆科植物。它的种皮坚硬而不透水,这导致了种子的物理休眠,为了克服坚硬的种皮强加的休眠,通过浸泡盐酸(HCl)软化坚硬的种皮。本研究评价了不同时间的HCL浸泡时间在10、20和30分钟对破茧蜂生理休眠的影响。结果表明,处理后种子的最终发芽率(FGP)分别较对照显著提高了55%、62%和68%,而对照仅为12%。HCL对种子萌发的影响是由于它能够破坏坚硬的种皮,从而导致种子的吸水和吸胀。结果表明,浸泡20 min和30 min处理种子的平均日发芽率在播种第7天显著提高,分别达到23%和24%,达到最有效发芽率,而对照组在播种第5天萌发停止,达到12%。结果表明,酸浸种子的平均发芽时间分别为9.66、9.13和8.62 d。处理后种子的最小MGT为30 min, 8.62 d。平均发芽时间较长的原因可能是由于使用了低浓度的盐酸,所以我建议使用浓度较高的酸来获得较低的平均发芽时间。
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引用次数: 0
Simulation Software as a Teaching Aid for Some Electronic Circuits Applications 仿真软件在某些电子电路教学中的应用
Pub Date : 2023-06-21 DOI: 10.51984/jopas.v22i1.2603
Zuhor Farag Edris
Electronic circuit technology is an important field with several scientific and practical applications. and that technology has been found that most modern electronic devices required nonlinear elements such as diodes, Transistors and operational amplifiers. Diodes have a variety of applications in electronics, including rectification, voltage demodulators, voltage regulation, logic gates and so on. In addition, transistors have many applications, which are widely used in electronics for amplification, switching, digital logic circuits, Power regulation, and oscillators. However, op-amps are electronic devices that can be used in a wide range of applications including Amplification, inverting, noninverting, summers, integrators, filtering, and comparators.This paper presents some examples of experimental simulation using Simulink software as a demonstration to students to make them understand the fundamentals of these nonlinear elements. Some experimental simulations were dealt with using diodes as a half-wave rectification, full-wave rectifier voltage regulator, and voltage limiter. However, the other experimental simulations dealt with OP-amp as an inverting, non-inverting, summer, subtractor, integrator and differentiator. Additionally, an operational amplifier is an important element in the design of active filters. Students can easily learn how to make experiment with this software in order to learn at their own time and pace. This helps visualize the fundaments of Op-Amp as well as any other physical system.
电子电路技术是一个具有多种科学和实际应用的重要领域。而且人们已经发现,大多数现代电子设备都需要非线性元件,如二极管、晶体管和运算放大器。二极管在电子学中有各种各样的应用,包括整流、电压解调器、电压调节、逻辑门等。此外,晶体管还有许多用途,广泛用于电子放大、开关、数字逻辑电路、功率调节和振荡器。然而,运算放大器是电子器件,可用于广泛的应用,包括放大,反相,非反相,夏季,积分器,滤波和比较器。本文用Simulink软件做了一些实验模拟的例子,让学生了解这些非线性元件的基本原理。用二极管作为半波整流、全波整流稳压器和限压器进行了一些实验模拟。然而,其他实验模拟处理运算放大器作为反相,非反相,夏季,减法器,积分器和微分器。此外,运算放大器是有源滤波器设计中的一个重要元素。学生可以很容易地学会如何用这个软件做实验,以便按照自己的时间和节奏学习。这有助于可视化运算放大器以及任何其他物理系统的基础。
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引用次数: 1
Methods of breaking seed physical dormancy and germination in native species of Alhagi graecorum Boiss (Al-Agool ) 土种Alhagi graecorum Boiss (Al-Agool)种子物理休眠和萌发的破茧方法
Pub Date : 2023-05-30 DOI: 10.51984/jopas.v22i1.2318
O. Sharashy
Alhagi graecorum is a plant  belonging to the Fabaceae family and grows as wild perennial shrubby species. Seeds of this species possess seed physical dormancy and need to be removed to enhance germination, breaking of dormancy treatments were imposed on seeds to improve germination. Treatments include scarification with  sulfuric acid ( H2SO4 ) for 10, 20, 30 and40 minutes. The results showed highly significant difference between control and all the treatments of germination percentage ( GP ) was 96%, 96%, 97% and 97%, respectively, while recorded in control only 12%. As for the mean daily germination ( MDG ), the results revealed that, there was a significant differences between all  treated seeds and control, which was the fastest and most effective seeds germination on the third day of sowing were 84, 89, 90, 92 % respectively, while in control was 0 %. Mean germination time    (MGT)  decreased in all treated seed but statistically same while significantly different from the control. The minimum time was recorded  for 20 and 30 minutes was 3.14 and 3.14 days respectively.            We conclude from the results obtained that, the use of concentrated sulfuric acid 98%  achieved the highest GP and  MDG and the lowest MGT. This is the efficient method of breaking seeds dormancy and germination for native species of Alhagi graecorum.
小檗属豆科植物,是一种野生多年生灌木。本种种子具有种子物理休眠,需要去除种子以促进萌发,对种子进行破休眠处理以提高萌发。处理方法包括用硫酸(H2SO4)切割10、20、30和40分钟。结果表明,不同处理的发芽率差异极显著,分别为96%、96%、97%和97%,而对照的发芽率仅为12%。在平均日发芽率(MDG)方面,各处理种子与对照差异显著,播后第3天种子最快、最有效发芽率分别为84%、89%、90%和92%,而对照为0%。各处理种子的平均发芽时间(MGT)均降低,但与对照相比差异有统计学意义。20分钟和30分钟的最短记录时间分别为3.14天和3.14天。结果表明,使用98%的浓硫酸可获得最高的GP和MDG,最低的MGT。这是一种有效的打破土生Alhagi graecorum种子休眠和萌发的方法。
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引用次数: 0
Experimental Efficiency of Single Pass Solar Air Heater 单道太阳能空气加热器的实验效率
Pub Date : 2023-05-28 DOI: 10.51984/jopas.v22i1.2192
Alsanossi M. Aboghrara, M. Shukra
The solar heat collector with the impingement of jet lower plate (SAHJF) experimentally designed and operated with typical parameters obtained from theoretical analysis to contained the maximum efficiency of the performance of thermal transfer. Then it was developed by replacing the flat absorber by the corrugated absorber (SAHJC) to enhance the efficiency. The flow rate of mass was selected equal and more than the typical value and the intensities of the solar radiation were chosen equal and less than the typical value to investigate the effect of both on the thermal transfer efficiency for SAHJF and SAHJC. Since the system works with a short range of temperature, the temperatures recorded were sensitive and needed a large accuracy. Therefore, an analysis method was proposed and applied to the data to be sure that it reflects the variance of temperature with different operating parameters. The correlation of temperature difference between the outlet and the inlet air with different flow rate and different intensities of irradiation for SAHJF and SAHJC was presented and compared.
采用理论分析得到的典型参数,对具有射流下板撞击的太阳能集热器进行了实验设计和运行,以实现传热性能的最大效率。在此基础上,采用波纹型吸收器代替平板吸收器来提高效率。选取质量流量等于或大于典型值,太阳辐射强度等于或小于典型值,考察两者对SAHJF和SAHJC换热效率的影响。由于该系统的工作温度范围很短,因此记录的温度很敏感,需要很高的精度。因此,提出了一种分析方法,并对数据进行了分析,以确保其反映了温度在不同运行参数下的变化。给出了不同流量和不同辐照强度下SAHJF和SAHJC出口和进口空气温差的相关性,并进行了比较。
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引用次数: 0
تصميم منظومة حصاد مياه الأمطار بالمدينة الجامعية لجامعة بنغازي، ليبيا. 在利比亚班加西大学设计雨水收集系统。
Pub Date : 2023-05-21 DOI: 10.51984/jopas.v22i1.2448
لبنى سليمان بن طاهر
تعاني مدينة بنغازي شأنها شأن المدن الليبية الاخرى من قلة مصادر المياه المتجددة, على هذا الاساس كان لابد من التفكير بمصادر وتقنيات لتعويض ذلك النقص, كاعتماد أنظمة حصاد مياه الأمطار؛ تهدف هذه الدراسة الى احتساب كميات حصاد مياه الأمطار من أسطح المباني والطرق والساحات لمقر المدينة الجامعية لجامعة بنغازي باستخدام تقنيتي الاستشعار عن بعد ونظم المعلومات الجغرافية, بمساعدة البرامج Google Earth وArcGIS10.5, ودراسة مدى تغطيتها للاستهلاك البشري للمياه بمقر المدينة الجامعية؛ وفق نتائج الدراسة نجد ان الحد الأقصى السنوي لمجموع تساقط الأمطار الشهري وفق بيانات محطة بنينا للأرصاد للفترة من 1970 الى 2020, بفترة رجوع 25 عام هو mm460 وما يتم تجميعه وحصاده من اسطح المباني والساحات والطرق من مياه الامطار بالسنة هو314789.58m3 , ولتجميع هذه الكميات تم تصميم شبكة من ثلاث خطوط بقطر 200mm وفقا للمواصفات الفنية الليبية، بميول للخطوط من 0.002 الى 0.009, لتلتقي بخزان تجميع دائري بقطرm27 وعمقm3؛ بنهاية هذه الدراسة نوصي ان تتبنى ادارة جامعة بنغازي بصفة خاصة والدولة بصفة عامة نظام حصاد مياه من الاسطح المختلفة وتوفير المتطلبات اللازمة له من نظام النقل والتخزين والتنقية, وتوعيه المواطنين وارشادهم وتشجيعهم لتنفيذ هذه التقنية وايضا شركات الانشاء والتعمير العامة والخاصة, وان تستغل مياه الامطار التي يتم حصادها من الاسطح, في إنشاء الحدائق والبحيرات لإضافة منظر جمالي للمدينة الجامعية, كما ان حصاد مياه الامطار من اسطح مباني الكليات ومرافق الجامعة, يمنع تراكم البرك بها فبتالي يتم حماية العناصر الانشائية من تأثير الرطوبة الضار وحماية المرافق من تسرب مياه الامطار بداخلها.
与利比亚其他城市一样,班加西市也缺乏可再生水资源,因此,必须考虑利用哪些资源和技术来弥补这一短缺,例如采用雨水收集系统;本研究的目的是利用遥感和地理信息系统技术,在谷歌地球和arcis10.5方案的协助下,计算班加西大学大学总部建筑物、道路和广场的雨水收集情况,并研究该大学本部的人类用水覆盖率;根据研究结果,从1970年至2020年(25年)期间,根据我们建造的气象站提供的数据,年均降水量为314789.58m3,收集收集的水量为3147889.5m3。在本研究结束时,我们建议班加西大学的行政部门,特别是国家行政部门,从不同的表面采集系统,提供运输、储存和净化系统的要求,提高公众对这一技术的认识、指导和鼓励,以及公共和私营建筑和建筑公司,利用雨水收集的雨水建立公园和湖泊,增加大学校园的美景,并从大学校园和大学设施的屋顶收集雨水。为了防止池塘里积聚,水分就能防止水分的有害影响,也能保护设施不被雨水冲走。
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引用次数: 0
Review: Electrochemical biosensors based on ZnO nanostructures 综述:基于ZnO纳米结构的电化学生物传感器
Pub Date : 2023-05-17 DOI: 10.51984/jopas.v22i1.1456
مريم خويطر
In the last few decades’ electrochemical biosensors have witnessed vast developments due to the broad range of different applications, including health care and medical diagnosis, environmental monitoring and assessment, food industry, and drug delivery. Integration of nanostructured material with different disciplines and expertise of electrochemistry, solid-state physics, material science, and biology has offered the opportunity of a future generation of highly rapid, sensitive, stable, selective, and novel electrochemical biosensor devices.Among metal oxide nanomaterials, ZnO nanostructures are one of the most important nanomaterials in today’s nanotechnology research. Such nanostructures have been studied intensely not only for their extraordinary structural, optical, and electronic properties but also for their prominent performance in diverse novel applications such as photonics, optics, electronics, drug delivery, cancer treatment, bio-imaging, etc. However, functionality of these nanomaterials is eventually dictated by the capability to govern their properties including shape, size, position, and crystalline structure on the nanosized scale.This review aims to update the outstanding advancement in the developments of the enzymatic and non-enzymatic biosensors using a different structure of ZnO nanomaterials. After a coverage of the basic principles of electrochemical biosensors, we highlight the basic features of ZnO as a potential anticancer agent. focused attention gives to functionalized biosensors based on ZnO nanostructures for detecting biological analytes, such as glucose, cholesterol, L-lactic acid, uric acid, metal ions, and pH.
在过去的几十年里,电化学生物传感器由于广泛的不同应用而取得了巨大的发展,包括医疗保健和医疗诊断,环境监测和评估,食品工业和药物输送。纳米结构材料与电化学、固态物理、材料科学和生物学的不同学科和专业知识的整合,为未来一代高度快速、敏感、稳定、选择性和新型电化学生物传感器设备提供了机会。在金属氧化物纳米材料中,氧化锌纳米结构是当今纳米技术研究中最重要的纳米材料之一。这种纳米结构不仅因其非凡的结构、光学和电子特性而受到广泛研究,而且在光子学、光学、电子学、药物输送、癌症治疗、生物成像等各种新应用中表现突出。然而,这些纳米材料的功能最终取决于在纳米尺度上控制其特性的能力,包括形状、大小、位置和晶体结构。本文综述了不同结构氧化锌纳米材料在酶和非酶生物传感器方面的研究进展。在介绍了电化学生物传感器的基本原理之后,我们重点介绍了氧化锌作为潜在抗癌剂的基本特征。重点研究了基于ZnO纳米结构的功能化生物传感器,用于检测葡萄糖、胆固醇、l -乳酸、尿酸、金属离子和pH等生物分析物。
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引用次数: 1
أثر البناء المخالف والتوسع العشوائي على البيئة الحضرية لمدينة سبها 违规建筑和肆意扩张对塞卜哈城市环境的影响
Pub Date : 2023-05-07 DOI: 10.51984/jopas.v22i1.2468
أحمد محمد الحضيري, محمد الولي عبدالقادر الشريف
صاحبت النمو الحضري السريع في عدد من الدول النامية بعض المشكلات الاجتماعية والاقتصادية والصحية والأمنية التي ما تنفك تتزايد مع ارتفاع معدلات النمو الحضري لا سيما إذا ما تم بعيداً عن الأسس والضوابط والمعايير التخطيطية والمعمارية. وقد ظهرت في العديد من المدن على اختلاف ظروفها مناطق ذات توسع عشوائي بسبب الضغط السكاني المتزايد سواء عن طريق النمو الطبيعي للسكان أم الهجرة إلى المدن بغية تحسين الأوضاع المعيشية والحصول على أفضل الخدمات والمرافق العامة، وقد كان لهذه المناطق آثار سلبية عمرانية وبيئية واجتماعية واقتصادية. هذه الورقة تحاول تسليط الضوء علي اثر التوسع العشوائي والمخالف علي البيئة الحضرية بمدينة سبها حيث يكتسب البحث اهمية كبيرة لكونه يتناول موضوعا يرتبط بالتخطيط العمراني السليم والذي يهدف الي توفير الخدمات الاساسية وتحسين الوضع السكني والبيئي والصحي للسكان بما يساعد اجهزة الدولة ومؤسساتها علي مكافحة العديد من المشكلات المصاحبة لهذه الظاهرة ومعرفة العوامل الكامنة وراءها وسبل معالجتها والحد من تفاقمها ووضع التصورات اللازمة لتفادي ظهور المزيد منها مستقبلا، كما توجت الدراسة في خاتمتها بمجموعة من النتائج والتوصيات لعلها تفيد صناع القرار للإسهام في حل هذه المشكلة.
一些发展中国家的城市快速增长伴随着一些社会、经济、卫生和安全问题,随着城市增长率的提高,特别是在远离规划和建筑基础、控制和标准的情况下,这些问题越来越多。由于人口压力越来越大,无论是通过人口的自然增长还是向城市迁移以改善生活条件和获得最佳公共服务和公用事业,许多城市都出现了人口扩张的地区,对城市、环境、社会和经济产生了不利影响。本文件试图突出塞卜哈市城市环境的不加区分和不公正扩展的影响,研究研究涉及到一个与健全的城市规划有关的主题,目的是提供基本服务和改善居民的住房、环境和健康状况,从而帮助国家机构和机构解决与这一现象有关的许多问题,了解这些问题的根源和解决这些问题的办法,并提出必要的看法,以避免今后出现更多的问题。这些建议可能有助于决策者帮助解决这一问题。
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引用次数: 0
Antibacterial Activity of Some Medicinal Plants against Some Bacteria Species and food spoilage bacteria 某些药用植物对某些细菌和食品腐败菌的抑菌活性
Pub Date : 2023-05-01 DOI: 10.51984/jopas.v22i1.2012
عبدالغني محمد ابوبكر, فاطمة خليفة القديري, نادية سالم الرويق, خليفة المهدي عبدالسلام علي
In the current study, some plants were selected to screen their potential antibacterial activity. For evaluating antibacterial activity, both aqueous and organic solvents) methanol and acetone (were used. The tested plants were Cymbopogon schoenanthus, Cotula cinerea, and Francoeuria crispa. Agar well diffusion method was used to test the antibacterial activity against two Gram negative (Brucella spp., Proteus spp.) and three Gram positive (Staphylococcus albus, Streptococcus spp., Bacillus subtilis) bacterial species. All extracts were effective antibacterial agents, being capable of inhibiting the growth of each examined bacteria. Based on the findings of this study, methanol, acetone and aqueous extracts of the leaves of C. schoenanthus, C. cinerea and F. crispa showed various antibacterial efficiency towards various bacterial species. Among plant extracts, the strongest antibacterial activity was shown by acetone extract of F. crispa against Streptococcus spp, with inhibition zone 27±1mm, Bacillus subtilis with inhibition zone of 26.27±4mm, followed by methanol against Streptococcus spp, and Brucella spp. with inhibition zone of 24±1 mm, 22±1mm respectively. Francoeuria crispa extracts exhibited the greatest antibacterial activity towards each of the evaluated bacteria, followed by Cymbopogon schoenanthus and Cotula cinerea. In comparison, methanol and acetone extracts showed pronounced inhibitory activity against both gram positive and gram negative bacteria. These findings demonstrate that certain plants may have antibacterial properties. This study suggests the possibility of applying Cymbopogon schoenanthus, Cotula cinerea, and Francoeuria crispa leaves as natural sources of antibacterial and may provide clues to clarify potential candidates for the future development of new chemotherapeutic drugs for the treatment of some infectious diseases.
在本研究中,我们选择了一些植物来筛选它们潜在的抗菌活性。为了评估抗菌活性,使用了水溶剂和有机溶剂甲醇和丙酮。试验植物为仙花Cymbopogon schoenanthus, Cotula cinerea和franeuria crispa。采用琼脂孔扩散法对2种革兰氏阴性菌(布鲁氏菌、变形杆菌)和3种革兰氏阳性菌(白色葡萄球菌、链球菌、枯草芽孢杆菌)进行抑菌活性测定。所有提取物都是有效的抗菌剂,能够抑制每一种被检测细菌的生长。本研究结果表明,香南花、灰绿花和crispa三种植物叶片的甲醇、丙酮和水提物对不同细菌的抑菌效果不同。在植物提取物中,葡萄球菌丙酮提取物对链球菌和枯草芽孢杆菌的抑菌活性最强,分别为27±1mm和26.27±4mm,其次是甲醇提取物,分别为24±1mm和22±1mm。crispa提取物对细菌的抑菌活性最高,其次是Cymbopogon schoenanthus和Cotula cinerea。甲醇和丙酮提取物对革兰氏阳性菌和革兰氏阴性菌均有明显的抑制作用。这些发现表明某些植物可能具有抗菌特性。该研究提示了将雪南草、牛耳草和crispa franeuria叶片作为天然抗菌药物的可能性,并可能为未来开发新的化疗药物治疗某些感染性疾病提供线索。
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引用次数: 0
تأثير عمليات العرض، التسويق والتصنيع على تركيز الهستامين في بعض انواع الأسماك 供应、营销和制造过程对某些鱼类中的司胺浓度的影响
Pub Date : 2023-05-01 DOI: 10.51984/jopas.v22i1.2382
احميده الغراري الزقطاط, هاجر عمر التومي
تعتبر عمليات تصنيع وتعليب المنتجات السمكية مثل التونة، السردين والماكريل من الصناعات الغذائية الهامة، حيث تستهلك بكميات كبيرة محلياً وعالمياً، وذلك بسبب مكوناتها الغذائية الجيدة إضافة الى سهولة تجهيزها وتحضيرها، إلا أنها قد تتسبب أحياناً في حدوث بعض حالات التسمم للمستهلك مثل حالات التسمم بالهستامين، وهو أحد أهم المخاطر والتحديات التي تواجه مصنعي المنتجات السمكية المعلبة والطازجة. تهدف هذه الدراسة في الجزء الأول الى تحديد مدى تأثير خطوات حفظ الأسماك على تركيز الهستامين في المنتجات السمكية بعينات من السردين والماكريل الطازج بأحد المصانع المحلية ومدى مطابقتها للمواصفات القياسية الليبية والعالمية. كما تضمن الجزء الثاني سحب عينات عشوائية من الأسماك الطازجة تونة، سردين وماكريل من سوق السمك بمدينة طرابلس خلال ثلاث فترات: الصباحية، الظهيرة والمسائية بغرض دراسة علاقة تأثير الفترة الزمنية لعرض الأسماك بالسوق وتركيز الهستامين. تم تقدير تركيز الهستامين في العينات بإستخدام تقنية .ELISA كان متوسط تركيز الهستامين في عينات السردين الطازج خلال مراحل التصنيع وهي مرحلة الإستلام، بعد الغسل، نزع الأحشاء والتنظيف والتجميد السريع عند درجة حرارة 40 تحت الصفر المئوي3.39 ،5.53 ، 8.56، 10.19، 12.11 مغ/100غ على التوالي. أما عينات الماكريل فكانت نتائج تركيز الهستامين< 0.02، 0.34، 0.14، 0.63، 3.05 مغ/100غ خلال تلك المراحل على التوالي. اتضح وجود فروق معنوية عند مستوى معنوية 5% بين مرحلتي الإستلام والمنتج النهائي. أما فيما يخص نتائج عينات الأسماك الطازجة من سوق السمك وهي تونة، سردين وماكريل خلال الفترات الثلاثة فتبين ارتفاع تركيز الهستامين وخاصة في الفترة المسائية ووجود فروق معنوية بينها، مما يدل على إن لعمليات تجهيز الأسماك وزيادة فترة العرض دور في زيادة تركيز الهستامين وعليه من الضروري جداً الاهتمام بعمليات النظافة وتقليل فترات العرض والتجهيز وإستخدام نظم إدارة سلامة الأغذية في المصانع وأسواق بيع الأسماك.
金枪鱼、沙丁鱼和磷虾等鱼类产品的制造和包装是一个重要的食品行业,由于其良好的营养成分以及易于加工和加工,在当地和全球范围内大量消费,但有时也会导致某些消费者中毒,例如海司胺中毒,这是罐装和新鲜鱼类产品制造商面临的主要风险和挑战之一。本研究报告第一部分的目的是确定养护措施对当地一家工厂的沙丁鱼和和鲜虾类鱼产品中的甲基苯丙胺浓度的影响,以及这些措施是否符合利比亚和全球标准。第二部分还包括在三个期间从的黎波里鱼类市场抽取新鲜金枪鱼金枪鱼、沙丁鱼和鲭鱼:上午、下午和下午,以研究鱼类供应时间对市场的影响和海司胺浓度的关系。elisa在制造过程中,新鲜沙丁鱼样品中的平均浓度分别为3.39、5.53、8.56、10.19毫克/100毫克。在这些阶段,磷虾的结果分别为0.02、0.34、0.14、0.63、3.05毫克/100克。在接收阶段和最终产品之间的道德差异为5%。至于在三个时期从金枪鱼金枪鱼、沙丁鱼和鲭鱼市场采集的新鲜鱼类样品,结果表明,特别是在晚上,司胺酮浓度很高,而且之间存在着精神上的差异,这表明鱼加工过程和供应期的增加对提高司胺酮浓度起了作用,因此,需要注意清洁工作,减少供应和加工时间,并在工厂和鱼类销售市场使用食品安全管理系统。
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引用次数: 0
A simple & Rapid Method For Detecting Bacterial Myrosinase Corresponding Protein Band 一种简便快速检测细菌黑芥子酶对应蛋白带的方法
Pub Date : 2023-03-15 DOI: 10.51984/jopas.v22i1.2210
Abdulhadi Albaser, فيجترا لونغ ان, نمرة نسار, نورالهدى عبدالقادر, جون روسيتر
Myrosinases have significant scientific and medical implications. Unfortunately, detection and purification of myrosinase from microbes requires the use of highly cost substrates (glucosinolates) such as sinigrin and expensive instruments such as Fast Protein Liquid Chromatography and or ion exchange chromatography. In this work, we used only 20 mL of bacterial culture supplemented with sinigrin (10 mM) to obtain partially purified myrosinase. The crude protein extract was loaded onto native polyacrylamide gel and putative myrosinase band was identified and eluted. This step successfully minimised the numbers of protein bands of bacterial crude extracts to be further analysed. The current method describes a simple, rapid and cost effective protocol for isolation and detection of active bacterial myrosinases. Furthermore, our method can be used as a purification step.
黑芥子酶具有重要的科学和医学意义。不幸的是,从微生物中检测和纯化黑芥子酶需要使用高成本的底物(硫代葡萄糖苷),如sinigrin和昂贵的仪器,如快速蛋白质液相色谱和/或离子交换色谱。在这项工作中,我们只使用了20 mL的细菌培养物,补充了紫荆素(10 mM),获得了部分纯化的黑芥子酶。将粗蛋白提取液装于天然聚丙烯酰胺凝胶上,鉴定并洗脱推定的黑芥子酶带。这一步骤成功地减少了细菌粗提取物中待进一步分析的蛋白质条带的数量。目前的方法描述了一种简单、快速和经济有效的分离和检测活性细菌黑芥子酶的方法。此外,我们的方法可以作为一个纯化步骤。
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引用次数: 1
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Journal of Pure &amp; Applied Sciences
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