Pub Date : 2021-03-10DOI: 10.22067/JPP.2021.32832.0
Ayoub Shafei, M. Rastgoo, E. I. Darbandi, A. Ghanbari
Introduction Herbicide safeners are chemical compounds used in combination with herbicides to make them "safer" that is, to reduce the effect of the herbicide on crop plants, and to improve selectivity between crop plants versus weed species being targeted by the herbicide. Herbicide safeners can be used to pretreat crop seeds prior to planting, or they can be sprayed on plants as a mixture with the herbicide. Salicylic acid (SA) and other salicylates are known to affect various physiological and biochemical activities of plants and may play a key role in regulating their growth and productivity. This hormone is synthesized by many plants and is accumulated in the plant tissues as the impact of unfavorable abiotic factors, contributing to the increase of plants resistance to stress. The aim of this study was to evaluate the efficacy of salicylic acid as a safener in corn tolerance to non-selective herbicide; sethoxydim, and the response of two weed species of johnsongrass (Sorghum halepense (L.) Pers.) and junglerice (Echinochloa colona (L.) Link) to it. Materials and methods The effect of sethoxydim (0.5, 22.5, 45, 94, 187 and 375 grams of active ingredient per hectare), and salicylic acid (in concentrations of 0, 1 and 2 mM) as factorial experiment in a completely randomized design were investigated to evaluate the response of fresh weight of corn, johnsongrass and junglerice. Salicylic acid treatment was used in seed priming form also. Pre-treatment of salicylic acid was done three days before herbicide spraying. Spraying different doses of sethoxydim herbicide in a complete five-leaf stage, was done using a mobile rail sprayer (Matabi, Spain) equipped with a flat-fan nozzle (8002), a delivery of 220 L ha-1 and a spray pressure of 200 kPa. To investigate the changes in photosynthesis in the treated plants, chlorophyll fluorescence of the leaves was evaluated on the sixth day after application of herbicides by PEA model fluorometer. Three weeks after spraying the herbicide, the shoots of the plants were removed from the surface of the pot and their fresh weight was measured. Analysis of variance was performed using generalized linear models (GLM) in SAS 9.2 software environment. Before performing analysis of variance to determine the normality of the data from Anderson-Darling test in Minitab ver. 17 were used, and in cases of non-normal data, Box-cox two-way power transformation was used in the same software. Analysis of variance, was performed as a factorial experiment for each plant to investigate the effect of the herbicide dose of sethoxydim and the concentration of salicylic acid and their interaction. The trend of wet weight changes of different species under the influence of different amounts of herbicide sethoxydim was investigated using the four parameters log-logistic equation.Results and discussion Dose-response curves of fresh weight of the treated plants showed that weeds were significantly affected by sethoxydim compared to corn.
除草剂安全剂是与除草剂联合使用以使其“更安全”的化合物,即减少除草剂对作物的影响,并提高作物与除草剂所针对的杂草物种之间的选择性。除草剂安全剂可用于在播种前对作物种子进行预处理,也可与除草剂混合喷洒在植物上。水杨酸(Salicylic acid, SA)和其他水杨酸类化合物影响植物的多种生理生化活性,可能在调节植物生长和生产力方面发挥关键作用。该激素由多种植物合成,在不利的非生物因素的影响下在植物组织中积累,有助于提高植物的抗逆性。本研究的目的是评价水杨酸作为一种安全剂对玉米非选择性除草剂的耐受性;四羟乙基醚(sethoxydim)及其对两种强生草(Sorghum halepense (L.))的响应棘球蚴(棘球蚴)和棘球蚴(棘球蚴)链接)。材料与方法采用完全随机设计的析因试验,以乙氧基肟(0.5、22.5、45、94、187和375 g /公顷)和水杨酸(浓度分别为0、1和2 mM)对玉米、强johngrass和山茱萸鲜重的影响进行研究。水杨酸处理也用于种子激发形式。在喷洒除草剂前3天对水杨酸进行预处理。采用移动式导轨式喷雾器(西班牙Matabi),在完整的五叶期喷洒不同剂量的乙氧基醚除草剂,喷雾器配备扁平扇形喷嘴(8002),喷施量为220 L ha-1,喷雾压力为200 kPa。为了研究施用除草剂后植株光合作用的变化,采用PEA模型荧光仪测定了施用除草剂后第6天叶片的叶绿素荧光。喷洒除草剂三周后,将植株的芽从花盆表面取出,并测量其鲜重。在SAS 9.2软件环境下,采用广义线性模型(GLM)进行方差分析。在进行方差分析以确定Minitab - ver中安德森-达林检验数据的正态性之前。在非正态数据情况下,在同一软件中使用Box-cox双向功率变换。采用方差分析作为析因试验,考察了乙氧基肟除草剂用量与水杨酸浓度的影响及其交互作用。采用四参数logistic方程研究了不同除草剂乙氧基肟用量对不同种属植物湿重变化的影响趋势。结果与讨论结果表明,与玉米相比,乙氧基肟对杂草鲜重的影响显著。在所有物种中,随着水杨酸浓度的增加,ED50增加。在所有三个水平水杨酸处理中,水杨酸作为引种处理的该参数最大,而丛林水稻的该参数最小。在2 mM / L水杨酸处理下,ED50的最高有效成分含量为210 g /公顷,在丛林水稻中,非水杨酸处理的ED50最低有效成分含量为31.5 g /公顷。玉米和杂草的Kutsky曲线显示,使用类固醇除草剂后,荧光叶绿素增加,但水杨酸处理会降低叶绿素荧光率,因此,在水杨酸处理最低(2 mM / L)的情况下,杂草和玉米在除草后没有草的对照中观察到。总的来说,本试验结果表明,在乙氧基肟的作用下,玉米、约翰逊草和丛林草3种植物对水杨酸叶面施用均表现出正响应。因此,为了使水杨酸作为一种安全剂,应在玉米中进行水杨酸种子处理。综上所述,水杨酸由于在窄叶植物中有乙氧基醚的使用,可以作为一种抗旱性物质。但作为一种安全剂,由于对作物和杂草的反应没有区别,不能与叶面喷施一样普遍使用。因此,在使用水杨酸作为乙氧基醚的免疫剂时,其种子处理效果较好。
{"title":"Evaluation of induction of sethoxydim tolerance in maize (Zea mays L.) using salicylic acid as a safener","authors":"Ayoub Shafei, M. Rastgoo, E. I. Darbandi, A. Ghanbari","doi":"10.22067/JPP.2021.32832.0","DOIUrl":"https://doi.org/10.22067/JPP.2021.32832.0","url":null,"abstract":"Introduction Herbicide safeners are chemical compounds used in combination with herbicides to make them \"safer\" that is, to reduce the effect of the herbicide on crop plants, and to improve selectivity between crop plants versus weed species being targeted by the herbicide. Herbicide safeners can be used to pretreat crop seeds prior to planting, or they can be sprayed on plants as a mixture with the herbicide. Salicylic acid (SA) and other salicylates are known to affect various physiological and biochemical activities of plants and may play a key role in regulating their growth and productivity. This hormone is synthesized by many plants and is accumulated in the plant tissues as the impact of unfavorable abiotic factors, contributing to the increase of plants resistance to stress. The aim of this study was to evaluate the efficacy of salicylic acid as a safener in corn tolerance to non-selective herbicide; sethoxydim, and the response of two weed species of johnsongrass (Sorghum halepense (L.) Pers.) and junglerice (Echinochloa colona (L.) Link) to it. Materials and methods The effect of sethoxydim (0.5, 22.5, 45, 94, 187 and 375 grams of active ingredient per hectare), and salicylic acid (in concentrations of 0, 1 and 2 mM) as factorial experiment in a completely randomized design were investigated to evaluate the response of fresh weight of corn, johnsongrass and junglerice. Salicylic acid treatment was used in seed priming form also. Pre-treatment of salicylic acid was done three days before herbicide spraying. Spraying different doses of sethoxydim herbicide in a complete five-leaf stage, was done using a mobile rail sprayer (Matabi, Spain) equipped with a flat-fan nozzle (8002), a delivery of 220 L ha-1 and a spray pressure of 200 kPa. To investigate the changes in photosynthesis in the treated plants, chlorophyll fluorescence of the leaves was evaluated on the sixth day after application of herbicides by PEA model fluorometer. Three weeks after spraying the herbicide, the shoots of the plants were removed from the surface of the pot and their fresh weight was measured. Analysis of variance was performed using generalized linear models (GLM) in SAS 9.2 software environment. Before performing analysis of variance to determine the normality of the data from Anderson-Darling test in Minitab ver. 17 were used, and in cases of non-normal data, Box-cox two-way power transformation was used in the same software. Analysis of variance, was performed as a factorial experiment for each plant to investigate the effect of the herbicide dose of sethoxydim and the concentration of salicylic acid and their interaction. The trend of wet weight changes of different species under the influence of different amounts of herbicide sethoxydim was investigated using the four parameters log-logistic equation.Results and discussion Dose-response curves of fresh weight of the treated plants showed that weeds were significantly affected by sethoxydim compared to corn.","PeriodicalId":16965,"journal":{"name":"Journal of Plant Protection","volume":"70 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73675930","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A field experiment was conducted to screen 20 rice genotypes against brown spot disease caused by Bipolaris oryzae under natural epiphytotic field conditions at Bangaun, Dang, Nepal, from June 2018 to March 2019. The experiment was carried out in a randomized complete block design with 3 replications. Sawa Mansuli was taken as susceptible check and Sabitri as a resistant check in the experiment. Disease assessment was done by calculating disease severity and Area Under the Disease Progress Curve (AUDPC). Among the evaluated genotypes, disease severity and AUDPC varied in the field experiments. Rice genotypes showed the resistance to highly susceptible reactions based on AUDPC value which ranged from 88.51 to 260.65. Among the evalauted rice genotypes in the field experiment, the highest mean AUDPC value was recorded with Basmati (260.65) followed by Radha-13 (172.80) and the lowest was recorded with Kathe Jhinuwa (88.51). Similarly, the highest severity rate was recorded with Basmati (25.91) followed by Radha-13 (21.00) and Tilki (20.75) and the lowest was recorded with Kathe Jhinuwa (11.03) which was at par with Radha-4 (11.11) followed by Sukhadhan-1 (12.02) and Sabitri (12.06). The highest grain yield was recorded with Sarju-52 (4.32 t/ha) followed by Sabitri (4.19 t/ha). Grain yield was negatively correlated with mean AUDPC by 14.77%. Kathe Jhinuwa, Radha-4, and Sabitri can be used for higher grain yield purposes under similar field conditions and also can be utilized as the source of resistance in a plant breeding program.
{"title":"Screening Rice Genotypes for Brown Spot Disease Resistance","authors":"A. Dhungana","doi":"10.52543/TJPP.15.2.1","DOIUrl":"https://doi.org/10.52543/TJPP.15.2.1","url":null,"abstract":"A field experiment was conducted to screen 20 rice genotypes against brown spot disease caused by Bipolaris oryzae under natural epiphytotic field conditions at Bangaun, Dang, Nepal, from June 2018 to March 2019. The experiment was carried out in a randomized complete block design with 3 replications. Sawa Mansuli was taken as susceptible check and Sabitri as a resistant check in the experiment. Disease assessment was done by calculating disease severity and Area Under the Disease Progress Curve (AUDPC). Among the evaluated genotypes, disease severity and AUDPC varied in the field experiments. Rice genotypes showed the resistance to highly susceptible reactions based on AUDPC value which ranged from 88.51 to 260.65. Among the evalauted rice genotypes in the field experiment, the highest mean AUDPC value was recorded with Basmati (260.65) followed by Radha-13 (172.80) and the lowest was recorded with Kathe Jhinuwa (88.51). Similarly, the highest severity rate was recorded with Basmati (25.91) followed by Radha-13 (21.00) and Tilki (20.75) and the lowest was recorded with Kathe Jhinuwa (11.03) which was at par with Radha-4 (11.11) followed by Sukhadhan-1 (12.02) and Sabitri (12.06). The highest grain yield was recorded with Sarju-52 (4.32 t/ha) followed by Sabitri (4.19 t/ha). Grain yield was negatively correlated with mean AUDPC by 14.77%. Kathe Jhinuwa, Radha-4, and Sabitri can be used for higher grain yield purposes under similar field conditions and also can be utilized as the source of resistance in a plant breeding program.","PeriodicalId":16965,"journal":{"name":"Journal of Plant Protection","volume":"661 1","pages":"29-39"},"PeriodicalIF":0.0,"publicationDate":"2020-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77687757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-07-22DOI: 10.22067/JPP.V34I3.85862
M. Esfandiari, S. Mohammadi, M. Jafari, Maryam Mousavinezhad Moghaddam
Introduction: Ants are the most ubiquitous insects on the earth with more than 12000 species which are classified into 21 subfamilies. Until now, more than 170 ant species have recorded in Iran. Mutualistic relationship between ants and scale insects have been reported in Iran. Such relationships between ants and insects are known as trophobiosis which is due to attraction of ants to honeydew of scale insects. Honeydew is a sugar-rich sticky liquid, secreted by aphids and some scale insects as they feed on plant sap. Ants provide protection from predators and parasitoids by building shelters around scale insect colonies. Such a relationship is mostly facultative and only a few taxa of mostly tropical or subtropical scale insects have obligate mutualism with ants. The coccoids with obligate mutualism display obvious behavioral and morphological adaptations for living with ants. Ants are also able to establish antagonistic interaction with honeydew-producing hemipterans and contribute positively to biological control by the suppression of pests. Materials and Methods: In a study during 2013-2014, colonies of the mealybug Paracoccus ficus Moghaddam, 2014 were investigated for mutualistic ants feeding on honeydew of the mealybug in fig orchards of Neyriz, Estahban and Shiraz cities of Fars province, South Iran. Samples were collected by hand, forceps and soft brush. The specimens were preserved in 75% alcohol in small glass vials and were transferred to the laboratory. The morphological keys were used for identification. Specimens were deposited in Insect and Mite Collection of Ahvaz, at Department of Plant Protection, Shahid Chamran University of Ahvaz. Results and Discussion: Totally, 6 species belonging to 3 subfamilies of ants were identified as follows: Myrmicinae: Monomorium abeillei Andre, 1881; Tetramorium sp.; Pheidole pallidula Nylander, 1849; Crematogaster antaris Forel, 1894; Formicinae: Cataglyphis lividus Andre, 1881; Dolichoderinae: Tapinoma simrothi Krausse, 1911. Among collected species, T. simrothi and P. pallidula were more frequent. T. simrothi has also recorded as most frequent mutualistic ants with aphids in Central Iran. Natural enemies exploit hemipterans which receive ant protection for their benefit. For example, some parasitoid wasps soliciting honeydew directly from aphids by antennation and mimicking ants during foraging in host patches. Researchers believe that preventing ants from colonies of Pseudococcidae scale insects is necessary for success in biological control of such pests. However, not all of ants have negative effects in biological control of pests. Predatory ants are less attracted to the honeydew and mostly feed on egg, larvae and adult insects. Moreover, some ants are important in pollination, soil improvement, and nutrient cycling. Conclusion: Behavioral and ecological should be carried on ants in pest management programs, and only if they have a negative influence on biological control of scale ins
{"title":"Mutualistic Ants (Hymenoptera: Formicidae) Associated with Paracoccus ficus in Fig Orchards of Fars Province, Iran","authors":"M. Esfandiari, S. Mohammadi, M. Jafari, Maryam Mousavinezhad Moghaddam","doi":"10.22067/JPP.V34I3.85862","DOIUrl":"https://doi.org/10.22067/JPP.V34I3.85862","url":null,"abstract":"Introduction: Ants are the most ubiquitous insects on the earth with more than 12000 species which are classified into 21 subfamilies. Until now, more than 170 ant species have recorded in Iran. Mutualistic relationship between ants and scale insects have been reported in Iran. Such relationships between ants and insects are known as trophobiosis which is due to attraction of ants to honeydew of scale insects. Honeydew is a sugar-rich sticky liquid, secreted by aphids and some scale insects as they feed on plant sap. Ants provide protection from predators and parasitoids by building shelters around scale insect colonies. Such a relationship is mostly facultative and only a few taxa of mostly tropical or subtropical scale insects have obligate mutualism with ants. The coccoids with obligate mutualism display obvious behavioral and morphological adaptations for living with ants. Ants are also able to establish antagonistic interaction with honeydew-producing hemipterans and contribute positively to biological control by the suppression of pests. \u0000Materials and Methods: In a study during 2013-2014, colonies of the mealybug Paracoccus ficus Moghaddam, 2014 were investigated for mutualistic ants feeding on honeydew of the mealybug in fig orchards of Neyriz, Estahban and Shiraz cities of Fars province, South Iran. Samples were collected by hand, forceps and soft brush. The specimens were preserved in 75% alcohol in small glass vials and were transferred to the laboratory. The morphological keys were used for identification. Specimens were deposited in Insect and Mite Collection of Ahvaz, at Department of Plant Protection, Shahid Chamran University of Ahvaz. \u0000Results and Discussion: Totally, 6 species belonging to 3 subfamilies of ants were identified as follows: \u0000Myrmicinae: Monomorium abeillei Andre, 1881; Tetramorium sp.; Pheidole pallidula Nylander, 1849; Crematogaster antaris Forel, 1894; Formicinae: Cataglyphis lividus Andre, 1881; Dolichoderinae: Tapinoma simrothi Krausse, 1911. \u0000Among collected species, T. simrothi and P. pallidula were more frequent. T. simrothi has also recorded as most frequent mutualistic ants with aphids in Central Iran. Natural enemies exploit hemipterans which receive ant protection for their benefit. For example, some parasitoid wasps soliciting honeydew directly from aphids by antennation and mimicking ants during foraging in host patches. Researchers believe that preventing ants from colonies of Pseudococcidae scale insects is necessary for success in biological control of such pests. However, not all of ants have negative effects in biological control of pests. Predatory ants are less attracted to the honeydew and mostly feed on egg, larvae and adult insects. Moreover, some ants are important in pollination, soil improvement, and nutrient cycling. \u0000Conclusion: Behavioral and ecological should be carried on ants in pest management programs, and only if they have a negative influence on biological control of scale ins","PeriodicalId":16965,"journal":{"name":"Journal of Plant Protection","volume":"311 1","pages":"357-360"},"PeriodicalIF":0.0,"publicationDate":"2020-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79977002","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-07-22DOI: 10.22067/JPP.V34I3.80386
M. R. S. Motlagh, M. Dashti
Introduction: Ricebakanae disease caused by Fusarium fujikuroiis one of the most important diseases of rice in Iran and in the world. Studies show that the disease has spread to a wide range of paddy fields worldwide, with losses in Japan up to 20%, in India up to 15%, and Thailand's northern and central areas have been reported to be 7.3% -14.7%. Symptoms include rice foot blackness and yellowish and then wilting of infected plants. The pathogen is more likely to attack the foot rot which is a sign of the abnormal growth of contaminated plants in the farm. Infected seedlings are slender and taller than healthy plants, and highly infected plants may die before or after transplantation. The tillering is reduced, consequently the leaves die in a short time. The fungus causes the disease threats the human and animal health through the production of phytotoxins. Identification of Fusarium species is currently confusing, on the other hand, several Fusarium species have always been isolated together with rice contaminated with the disease. Therefore, it is not clear which species of this fungus are the main reason of the disease. This fungus is soil-borne and has a long life in heavy soils. It has also a global expansion and is active in most parts of the world. Rice foot rot disease in all major rice producing countries in the world is considered as a seed disease and the transmission of the disease agent from one season to another season is mainly due to contaminated seed, but soil-borne fungus can be as well. Currently, treating the seeds with fungicides is the best method to control this disease, however using chemical pesticides can lead to environmental pollution. In this situation, it is important to achieve a healthy alternate method. For this purpose, biological control is one of the ways in which today a large investment is being made around the world. Materials and Methods: Samples of rice exhibiting the symptoms of bakanae were randomly collected from different parts of Gilan province, Iran. Pieces of organs with rot symptoms were cut and they were surface-disinfected after washing with 0.5% sodium hypochlorite solution. At the next step, they were washed with distilled water and dried on filter papers. Then, they were cultured in Petri dishes containing potato dextrose agar (PDA) culture medium and were placed in an incubator at 28°C for 3-5 days. Afterward, they were placed on a water-agar medium for purification and morphological identification. In this research, from a total of 80 samples collected from rice farms in Gilan province, 18 bacterial strains were isolated and the antagonistic ability of 8 strains of bacteria in the laboratory and greenhouse was investigated. For this purpose, in laboratory, dual culture method, volatile compounds, siderophore and antibiotic production were used. These bacterial strains were inoculated into rice under greenhouse conditions, and then the severity of the disease was determined in the tested tre
因此,尽可能多地分离和鉴定这些菌株,有望使生物防治方法在水稻冠腐病防治管理中发挥更大的作用。
{"title":"Biological Control of Fusarium fujikuroi, the Causal Agent of Bakanae Using Some Antagonistic Bacteria in Gilan Province","authors":"M. R. S. Motlagh, M. Dashti","doi":"10.22067/JPP.V34I3.80386","DOIUrl":"https://doi.org/10.22067/JPP.V34I3.80386","url":null,"abstract":"Introduction: Ricebakanae disease caused by Fusarium fujikuroiis one of the most important diseases of rice in Iran and in the world. Studies show that the disease has spread to a wide range of paddy fields worldwide, with losses in Japan up to 20%, in India up to 15%, and Thailand's northern and central areas have been reported to be 7.3% -14.7%. Symptoms include rice foot blackness and yellowish and then wilting of infected plants. The pathogen is more likely to attack the foot rot which is a sign of the abnormal growth of contaminated plants in the farm. Infected seedlings are slender and taller than healthy plants, and highly infected plants may die before or after transplantation. The tillering is reduced, consequently the leaves die in a short time. The fungus causes the disease threats the human and animal health through the production of phytotoxins. Identification of Fusarium species is currently confusing, on the other hand, several Fusarium species have always been isolated together with rice contaminated with the disease. Therefore, it is not clear which species of this fungus are the main reason of the disease. This fungus is soil-borne and has a long life in heavy soils. It has also a global expansion and is active in most parts of the world. Rice foot rot disease in all major rice producing countries in the world is considered as a seed disease and the transmission of the disease agent from one season to another season is mainly due to contaminated seed, but soil-borne fungus can be as well. Currently, treating the seeds with fungicides is the best method to control this disease, however using chemical pesticides can lead to environmental pollution. In this situation, it is important to achieve a healthy alternate method. For this purpose, biological control is one of the ways in which today a large investment is being made around the world. Materials and Methods: Samples of rice exhibiting the symptoms of bakanae were randomly collected from different parts of Gilan province, Iran. Pieces of organs with rot symptoms were cut and they were surface-disinfected after washing with 0.5% sodium hypochlorite solution. At the next step, they were washed with distilled water and dried on filter papers. Then, they were cultured in Petri dishes containing potato dextrose agar (PDA) culture medium and were placed in an incubator at 28°C for 3-5 days. Afterward, they were placed on a water-agar medium for purification and morphological identification. In this research, from a total of 80 samples collected from rice farms in Gilan province, 18 bacterial strains were isolated and the antagonistic ability of 8 strains of bacteria in the laboratory and greenhouse was investigated. For this purpose, in laboratory, dual culture method, volatile compounds, siderophore and antibiotic production were used. These bacterial strains were inoculated into rice under greenhouse conditions, and then the severity of the disease was determined in the tested tre","PeriodicalId":16965,"journal":{"name":"Journal of Plant Protection","volume":"31 1","pages":"287-295"},"PeriodicalIF":0.0,"publicationDate":"2020-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78671116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2011-07-01DOI: 10.21608/JPPP.2011.86515
G. Abdu-Allah, V. Mommaerts, G. Smagghe
Under laboratory conditions, the acute toxicity of spinosad on adult workers of bumble bee Bombus terrestris L.was investigated through 96 hrs post-treatment by using three different exposure methods; orally, wet and dry contact. The results indicated that within 24 hrs, the 1/1 and 1/10 of maximum field recommended concentration (MFRC) of spinosad caused 100 % and 12-65 % mortality, respectively the three methods. While, the mortality in the control insects did not exceed 5 %. The highest LC50 value was detected in dry contact method at 6 hrs, the LC50=1046.15 ppm (2.62 MFRC). However, the lowest one in oral method was detected after 96 hrs, the LC50 values was 5.03 ppm (0.01 MFRC). At concentration 0.01 MFRC with dry contact,LT50 was 73 days but in the oral method had 14 days. The chronic effects of 1/100, 1/1000, 1/10000 of MFRC using the same three exposure methods were evaluated. The 1/100 of MFRC in oral method caused significant mortality in worker through 8 weeks. Moreover, reduction in survival of workers, drones produced, male delay emergency, and numbers of dead larvae. However, no significant differences were found between the control and the other treatment 1/1000 and 1/10000 MFRC using oral assay. In dry contact method, the concentrations from 1/1000 to 1/10 of MFRC spinosad had no negative effects. In wet contact method the concentrations 1/100 and 1/1000 had no negative effect, but the concentration 1/10 caused decreasing survival workers. Our results suggested that spinosad was highly toxic in wet contact method, although more safe in dry contact method to bumble bees under worse case laboratory condition with MFRC.
{"title":"Acute and chronic effects of spinosad on bumble bees, Bombus terrestris L. under laboratory conditions","authors":"G. Abdu-Allah, V. Mommaerts, G. Smagghe","doi":"10.21608/JPPP.2011.86515","DOIUrl":"https://doi.org/10.21608/JPPP.2011.86515","url":null,"abstract":"Under laboratory conditions, the acute toxicity of spinosad on adult workers of bumble bee Bombus terrestris L.was investigated through 96 hrs post-treatment by using three different exposure methods; orally, wet and dry contact. The results indicated that within 24 hrs, the 1/1 and 1/10 of maximum field recommended concentration (MFRC) of spinosad caused 100 % and 12-65 % mortality, respectively the three methods. While, the mortality in the control insects did not exceed 5 %. The highest LC50 value was detected in dry contact method at 6 hrs, the LC50=1046.15 ppm (2.62 MFRC). However, the lowest one in oral method was detected after 96 hrs, the LC50 values was 5.03 ppm (0.01 MFRC). At concentration 0.01 MFRC with dry contact,LT50 was 73 days but in the oral method had 14 days. The chronic effects of 1/100, 1/1000, 1/10000 of MFRC using the same three exposure methods were evaluated. The 1/100 of MFRC in oral method caused significant mortality in worker through 8 weeks. Moreover, reduction in survival of workers, drones produced, male delay emergency, and numbers of dead larvae. However, no significant differences were found between the control and the other treatment 1/1000 and 1/10000 MFRC using oral assay. In dry contact method, the concentrations from 1/1000 to 1/10 of MFRC spinosad had no negative effects. In wet contact method the concentrations 1/100 and 1/1000 had no negative effect, but the concentration 1/10 caused decreasing survival workers. Our results suggested that spinosad was highly toxic in wet contact method, although more safe in dry contact method to bumble bees under worse case laboratory condition with MFRC.","PeriodicalId":16965,"journal":{"name":"Journal of Plant Protection","volume":"137 1","pages":"677-690"},"PeriodicalIF":0.0,"publicationDate":"2011-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84750703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2011-07-01DOI: 10.21608/JPPP.2011.86513
M. Asran, M. Aboul-Nasr
Fusarium verticilliodes (Sacc.) Nirenberg (G) is a well known phytopathogenic fungus capable to infect a wide range of plants including maize. In addition; its toxic metabolites fumonisin (FB1 and FB2) are associated with Equina Leukoencephalo Malacia and induced hepatotoxic and carcinogenic effects in animals. Twenty two isolates of F. verticilliodes obtained from corn samples and grown on Barley medium, were added to sterile soil two weeks before sowing maize grains to test their ability to cause corn seedling damping-off. Thirteen isolates caused pre-emergence dampingoff, and two of them were significant. In addition one F. verticilliodes isolate was significantly affecting post emergency and four isolates exhibited the highest disease index. F. verticilliodes isolates which caused significant pre and post emergence damping-off as well as disease index were found to be fumonisin producers. Fourteen out of twenty two detached and moistened maize leaves showed necrotic lesions when they inoculated by F. verticilliodes spore suspensions. Further more the crude extracts of the tested F. verticilliodes isolates showed leaf lesions when they were spotted on the moisted maize leaf cuts, in addition to FB1 standard. Twelve of the pathogenic F. verticilliodes isolates produced fumonisins (eight; FB1 and four; FB1 & FB2); whereas fumonisins could not been detected in only two of the pathogenic isolates according to TLC.. These results suggest the great role of fumonisins that may be played as a virulence factor of F. verticilliodes on maize.
{"title":"Virulence of Fusarium verticilliodes,Sacc., nirenberg ,G,isolates on maize plants associated with fumonisins production","authors":"M. Asran, M. Aboul-Nasr","doi":"10.21608/JPPP.2011.86513","DOIUrl":"https://doi.org/10.21608/JPPP.2011.86513","url":null,"abstract":"Fusarium verticilliodes (Sacc.) Nirenberg (G) is a well known phytopathogenic fungus capable to infect a wide range of plants including maize. In addition; its toxic metabolites fumonisin (FB1 and FB2) are associated with Equina Leukoencephalo Malacia and induced hepatotoxic and carcinogenic effects in animals. Twenty two isolates of F. verticilliodes obtained from corn samples and grown on Barley medium, were added to sterile soil two weeks before sowing maize grains to test their ability to cause corn seedling damping-off. Thirteen isolates caused pre-emergence dampingoff, and two of them were significant. In addition one F. verticilliodes isolate was significantly affecting post emergency and four isolates exhibited the highest disease index. F. verticilliodes isolates which caused significant pre and post emergence damping-off as well as disease index were found to be fumonisin producers. Fourteen out of twenty two detached and moistened maize leaves showed necrotic lesions when they inoculated by F. verticilliodes spore suspensions. Further more the crude extracts of the tested F. verticilliodes isolates showed leaf lesions when they were spotted on the moisted maize leaf cuts, in addition to FB1 standard. Twelve of the pathogenic F. verticilliodes isolates produced fumonisins (eight; FB1 and four; FB1 & FB2); whereas fumonisins could not been detected in only two of the pathogenic isolates according to TLC.. These results suggest the great role of fumonisins that may be played as a virulence factor of F. verticilliodes on maize.","PeriodicalId":16965,"journal":{"name":"Journal of Plant Protection","volume":"24 1","pages":"653-662"},"PeriodicalIF":0.0,"publicationDate":"2011-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78217332","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2011-06-01DOI: 10.21608/JPPP.2011.86511
S. Allam
In Menoufiya Governorate, a field experiment was conducted as a completely randomized design, in spring period during the two successive season 2010 and 2011 using Kidney bean plantation. four different treatment were conducted as :Kidney bean plants alone in addition to the same plant intercropped on Mint, Fennel and Black cumin. The main crop, Kidney bean, plants were sown on 4 different spaces i.e.10, 20, 30 and 40cm. Kidney bean plants were investigated for abundance of the different stages of the two spotted spider mite Tetranychus urticae Koch :eggs, immatures and adults, as wells their predators Phytosiulus persimilis Athias-Henriot Euseius scutaulis Athias-Henriot (Family: Phytoseiidae). Inspection and investigation was conducted every two weeks: 15,30,45,60,75 and 90 days from sowing. Results showed that the mean values of T. urticae during the growth period clearly increased in the space of 10cm between plants while the reverse trend occurred with the space 40cm. Results showed also, there was a significant difference between the mean numbers of eggs, immatures and adults of Tetranychus urticae Koch infesting Kedney bean in different spaces during sowing.
{"title":"Utilization intercropping in the reduction of the two spotted spider mite Tetranchus urticae koch,infesting kidney bean","authors":"S. Allam","doi":"10.21608/JPPP.2011.86511","DOIUrl":"https://doi.org/10.21608/JPPP.2011.86511","url":null,"abstract":"In Menoufiya Governorate, a field experiment was conducted as a completely randomized design, in spring period during the two successive season 2010 and 2011 using Kidney bean plantation. four different treatment were conducted as :Kidney bean plants alone in addition to the same plant intercropped on Mint, Fennel and Black cumin. The main crop, Kidney bean, plants were sown on 4 different spaces i.e.10, 20, 30 and 40cm. Kidney bean plants were investigated for abundance of the different stages of the two spotted spider mite Tetranychus urticae Koch :eggs, immatures and adults, as wells their predators Phytosiulus persimilis Athias-Henriot Euseius scutaulis Athias-Henriot (Family: Phytoseiidae). Inspection and investigation was conducted every two weeks: 15,30,45,60,75 and 90 days from sowing. Results showed that the mean values of T. urticae during the growth period clearly increased in the space of 10cm between plants while the reverse trend occurred with the space 40cm. Results showed also, there was a significant difference between the mean numbers of eggs, immatures and adults of Tetranychus urticae Koch infesting Kedney bean in different spaces during sowing.","PeriodicalId":16965,"journal":{"name":"Journal of Plant Protection","volume":"17 1","pages":"645-652"},"PeriodicalIF":0.0,"publicationDate":"2011-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82018163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2011-06-01DOI: 10.21608/JPPP.2011.86505
A. Said
The objective of present work was to compare the field efficiency of three tested pesticides, Selecron (profenofos), Marshal (carbosulfan) and Radiant (spinetoram), against larvae and adults of the tortoise beetle Cassida vittata (Vill.) inhabiting sugar beet fields in Sharkia Governorate during 2008/2009 and 2009/2010 sugar beet growing seasons. Data obtained revealed that when mortality rates were considered, Selecrone and Marshal were the most efficient compounds against larvae and adults of C. vittata. However, Radiant demonstrated a moderate toxic effect. Marshal and Selecrone showed an increase in sugar percentage (16.75 % and 14.5 % respectively) and total soluble solids (TSS %) (18 %, 17 % respectively) in the first season, while in the second season the increase in sugar percentage were (19.8 % and 19.10 % respectively), and TSS % was (22 % and 20 %, respectively). In the second season Radiant showed less percentages of sugar and (TSS) which are 16.70 % and 18.50 %, respectively.
{"title":"Effect of certain insecticides against sugar beet beetle Cassida vittata, vill., Coleoptera: Chrysomelidae, inhabiting sugar beet fields","authors":"A. Said","doi":"10.21608/JPPP.2011.86505","DOIUrl":"https://doi.org/10.21608/JPPP.2011.86505","url":null,"abstract":"The objective of present work was to compare the field efficiency of three tested pesticides, Selecron (profenofos), Marshal (carbosulfan) and Radiant (spinetoram), against larvae and adults of the tortoise beetle Cassida vittata (Vill.) inhabiting sugar beet fields in Sharkia Governorate during 2008/2009 and 2009/2010 sugar beet growing seasons. Data obtained revealed that when mortality rates were considered, Selecrone and Marshal were the most efficient compounds against larvae and adults of C. vittata. However, Radiant demonstrated a moderate toxic effect. Marshal and Selecrone showed an increase in sugar percentage (16.75 % and 14.5 % respectively) and total soluble solids (TSS %) (18 %, 17 % respectively) in the first season, while in the second season the increase in sugar percentage were (19.8 % and 19.10 % respectively), and TSS % was (22 % and 20 %, respectively). In the second season Radiant showed less percentages of sugar and (TSS) which are 16.70 % and 18.50 %, respectively.","PeriodicalId":16965,"journal":{"name":"Journal of Plant Protection","volume":"100 1","pages":"597-607"},"PeriodicalIF":0.0,"publicationDate":"2011-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80236738","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2011-04-01DOI: 10.21608/JPPP.2011.86477
M. Mohamed
Some plant families (Fabaceae,Asteraceae, Brassicaceae, Poaceae, Solenaceae, Cucurbitaceae....) have different allelopathic capability to produce and exude allelochemicals into their environments to suppress the growth of annual weeds in their surrounding area. In this study, wheat ( Triticum aestivum L.), corn ( Zea mays L.) and their associated annual weeds were exposed to the allelochemicals arised from their previousing crops during the crop rotation. The summer crops prior to wheat were maize, sorghum, panicum, alfalfa, cowpea, sunflower. While the winter crops prior to maize were safflower, barley, canola, rye, oat, wheat, respectively. For this target two field experiments were conducted at Maryout Experimental Station, Desert Research Center during 2003-2005 winter and summer seasons. Weed species associated to wheat exhibited substantial significant reduction in its fresh and dry weights as a result of growing wheat subsequent to maize, sorghum, alfalfa, sunflower, panicum and cowpea, respectively. Wheat grain yield and biological yield showed significant increases with the same species order after the decomposition period of crop residues. . The fresh and dry weights of weed species associated to maize were reduced significantly by sowing maize subsequent to wheat, barley, oat, rye and safflower, respectively. Maize grain yield and biological yield showed significant increases with the same species order, respectively. The families of Poaceae and Fabaceae was the best families that were used before wheat and maize in control of annual weeds associated with crops compared to other families. This work aimed to study the allelopathic capability of some plant families that precede each of wheat or maize in the agricultural cycle, to control the annual weeds associated to wheat or maize and same time to increase their growth and productivity.
{"title":"Allelopathic capability of some plant families on safe weed control in certain crops","authors":"M. Mohamed","doi":"10.21608/JPPP.2011.86477","DOIUrl":"https://doi.org/10.21608/JPPP.2011.86477","url":null,"abstract":"Some plant families (Fabaceae,Asteraceae, Brassicaceae, Poaceae, Solenaceae, Cucurbitaceae....) have different allelopathic capability to produce and exude allelochemicals into their environments to suppress the growth of annual weeds in their surrounding area. In this study, wheat ( Triticum aestivum L.), corn ( Zea mays L.) and their associated annual weeds were exposed to the allelochemicals arised from their previousing crops during the crop rotation. The summer crops prior to wheat were maize, sorghum, panicum, alfalfa, cowpea, sunflower. While the winter crops prior to maize were safflower, barley, canola, rye, oat, wheat, respectively. For this target two field experiments were conducted at Maryout Experimental Station, Desert Research Center during 2003-2005 winter and summer seasons. Weed species associated to wheat exhibited substantial significant reduction in its fresh and dry weights as a result of growing wheat subsequent to maize, sorghum, alfalfa, sunflower, panicum and cowpea, respectively. Wheat grain yield and biological yield showed significant increases with the same species order after the decomposition period of crop residues. . The fresh and dry weights of weed species associated to maize were reduced significantly by sowing maize subsequent to wheat, barley, oat, rye and safflower, respectively. Maize grain yield and biological yield showed significant increases with the same species order, respectively. The families of Poaceae and Fabaceae was the best families that were used before wheat and maize in control of annual weeds associated with crops compared to other families. This work aimed to study the allelopathic capability of some plant families that precede each of wheat or maize in the agricultural cycle, to control the annual weeds associated to wheat or maize and same time to increase their growth and productivity.","PeriodicalId":16965,"journal":{"name":"Journal of Plant Protection","volume":"39 1","pages":"463-473"},"PeriodicalIF":0.0,"publicationDate":"2011-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84363674","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2011-03-01DOI: 10.21608/JPPP.2011.86422
S. EL-Sayed
Some microelements, namely zinc, manganese and calcium were used singly or in combination with typical fungicide, Rhizolex-T 50 to investigate their effect towards the control of faba bean root-rot caused by Rhizoctonia solani. Laboratory, greenhouse and field studies were conducted during 2008/2009 & 2009/2010 seasons using the faba bean cultivar, Giza 40. Results of in vitro studies showed that microelements or Rhizolex-T significantly inhibited the mycelial growth of the pathogen, R. solani when compared with the untreated control.Under greenhouse conditions, all isolatedfungi proved to be pathogenic and caused preand postemergence damping-off while R. solani being the most virulent. All some microelements or the fungicide tested as seed treatments for field experiments led to an increase in the plant height . Rhizolex-T 50 followed by zinc were the most effective treatments in reducing the incidence of faba bean dampingoff. Calcium followed by zinc were the most effective in increasing the number of pods per plant, the weight of 100 seeds, and seed yield / plot. The most effective seed treatment in reducing the root-rot disease incidence was Rhizolex-T followed by zinc and then calcium.
{"title":"Control of faba bean root rot disease by using micro-elements and the fungicide, rhizolex-T","authors":"S. EL-Sayed","doi":"10.21608/JPPP.2011.86422","DOIUrl":"https://doi.org/10.21608/JPPP.2011.86422","url":null,"abstract":"Some microelements, namely zinc, manganese and calcium were used singly or in combination with typical fungicide, Rhizolex-T 50 to investigate their effect towards the control of faba bean root-rot caused by Rhizoctonia solani. Laboratory, greenhouse and field studies were conducted during 2008/2009 & 2009/2010 seasons using the faba bean cultivar, Giza 40. Results of in vitro studies showed that microelements or Rhizolex-T significantly inhibited the mycelial growth of the pathogen, R. solani when compared with the untreated control.Under greenhouse conditions, all isolatedfungi proved to be pathogenic and caused preand postemergence damping-off while R. solani being the most virulent. All some microelements or the fungicide tested as seed treatments for field experiments led to an increase in the plant height . Rhizolex-T 50 followed by zinc were the most effective treatments in reducing the incidence of faba bean dampingoff. Calcium followed by zinc were the most effective in increasing the number of pods per plant, the weight of 100 seeds, and seed yield / plot. The most effective seed treatment in reducing the root-rot disease incidence was Rhizolex-T followed by zinc and then calcium.","PeriodicalId":16965,"journal":{"name":"Journal of Plant Protection","volume":"04 1","pages":"295-304"},"PeriodicalIF":0.0,"publicationDate":"2011-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86285847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}