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Evaluation of non-host crops as trap crops to reduce Orobanche seed bank in tomato fields 非寄主作物作为陷阱作物在番茄田减少Orobanche种子库的评价
Pub Date : 2021-09-01 DOI: 10.26655/jrweedsci.2021.2.7
B. Acharya, A. Bista, Sanju Jyawali, Nabin Kumar Darai
Orobanche species, commonly known as broomrape, are root holo-parasite of the family Orobanchaceae.  In Nepal two species, Orobanche aegyptiaca and O. cernua are causing threat to wide range of important vegetables and crops of family Brassicaceae and Solanaceae. To evaluate non-host plants as potential trap crops, different crop plants were tested in two Orobanche infested tomato fields of Lalbandi, Sarlahi, South-Eastern part of Nepal in 2018 -19. Orobanche seed density in soil samples collected from plots before planting and after harvest of each crop species were recorded and compared for the seed bank. On the basis of Orobanche seed bank reduction in both the fields, the investigated crop species like Capsicum frutescens, Cicer arietinum, Lens culinaris and Vicia faba were classified as highly potential trap crops and reduced seed bank from 52 to 70%. Crops like Cuminum cyminum, Daucus carota, Foeniculum vulgare, Phaseolus vulgaris, Sessamum orientale and Trigonella foenum-graecum were classified as moderately potential trap crops and reduced Orobanche seed bank from 41 to 52 %. The other tested crops like Allium cepa, Allium sativum, Coriandrum sativum, Glycine max, Hordeum vulgare, Pisum sativum, Solanum tuberosum, Raphanus sativus are classified as non- potential trap crops. Among all the tested crops Capsicum frutescens, Cicer arietinum, Lens culinaris and Vicia faba were identified as the potential trap crop to reduce Orobanche seed bank in infested tomato fields.
龙葵属,俗称帚状花,是龙葵科的根寄生植物。在尼泊尔,两个物种埃及斑蝽和cernua斑蝽正在对十字花科和茄科的多种重要蔬菜和作物造成威胁。为了评估非寄主植物作为潜在诱捕作物的潜力,2018 - 2019年在尼泊尔东南部萨拉希地区拉尔班迪的两个Orobanche侵染番茄田对不同作物进行了试验。在每一种作物种植前和收获后收集的土壤样本中记录了土中小麦种子的密度,并与种子库进行了比较。在两地Orobanche种子库减少的基础上,将调查作物辣椒(Capsicum frutescens)、西芹(Cicer arietinum)、Lens culinaris和蚕豆(Vicia faba)分类为高潜力诱捕作物,种子库减少了52%至70%。Cuminum cyminum、Daucus carota、Foeniculum vulgare、Phaseolus vulgaris、Sessamum orientale和Trigonella foenum-graecum等作物被列为中等潜在陷阱作物,使Orobanche种子库从41%减少到52%。其他被试作物,如葱、葱、芫荽、甘氨酸、普通Hordeum vulgare、Pisum sativum、Solanum tuberosum、Raphanus sativus,被归为非势阱作物。在所有试验作物中,辣椒(Capsicum frutescens)、西芹(Cicer arietinum)、蓝丝(Lens culinaris)和蚕豆(Vicia faba)被确定为减少番茄侵染地Orobanche种子库的潜在诱捕作物。
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引用次数: 0
Allelopathic effect of crude plant extract on Ipomoea purpurea L. 植物粗提物对紫薯蓣的化感作用。
Pub Date : 2021-06-01 DOI: 10.26655/JRWEEDSCI.2021.2.5
Lucas A Castro, Danillo Oliveira Beltrão, Patrícia Guimarães Santos, Y. B. Carvalho, Nélio Renato Nogueira Filho, J. P. Costa, E. Marques
Weeds are among the main phytosanitary problems and are responsible for numerous losses in agriculture. Integrated management has become increasingly important in weed control. Therefore, the present study aimed to investigate the allelopathic effect of 24 plants on morning glory (Ipomoea purpurea L.). The tests were performed in a completely randomized design, both in in vitro and in vivo experiments. The in vitro germination data were used to calculate the time and germination index, and in the in vivo experiment, the length and fresh weight of the aerial part and root of the seedlings were evaluated, with subsequent comparison of averages by ANOVA. The plant extracts used exerted allelopathy, delaying (negative) and advancing (positive) the germination of this species. The extracts of banana, black plum, carqueja, Mexican sunflower, rosemary, guaco, lavender, lemongrass and garden mint resulted in high germination delays of up to +5 days. Rosemary, garden mint, Mexican sunflower and guaco extracts also reduced the germination rate by up to -10.5%. None of the tested extracts reduced the length of the aerial part of morning glory. In all treatments, shorter root length was observed. The extracts of lemongrass, rosemary and guaco reduced the dry weight of the aerial part, and in all treatments a lower fresh weight of roots was observed, except for the treatment with guaco. It can be concluded that the germination or growth of some problematic weeds such as I. purpurea may be affected by allelochemical extracts from different plants such as bananas, black plum, boldo, Mexican sunflower, rosemary, guaco, lavender, lemongrass, and garden mint.
杂草是主要的植物检疫问题之一,是造成农业大量损失的原因。综合管理在杂草控制中变得越来越重要。因此,本研究旨在探讨24种植物对牵牛花(Ipomoea purpurea L.)的化感作用。实验采用完全随机设计,包括体内和体外实验。体外萌发数据计算萌发时间和萌发指数,体内实验中评估幼苗地上部和根部的长度和鲜重,随后采用方差分析比较平均值。所使用的植物提取物发挥化感作用,延迟(阴性)和促进(阳性)该物种的萌发。香蕉、黑梅、卡克哈树、墨西哥向日葵、迷迭香、鳄梨、薰衣草、柠檬草和花园薄荷的提取物导致萌发延迟高达5天。迷迭香、花园薄荷、墨西哥向日葵和鳄梨提取物也使发芽率降低了-10.5%。测试的提取物都没有减少牵牛花的空中部分的长度。在所有处理中,根长均较短。柠檬草、迷迭香和鳄梨提取物降低了地上部分的干重,除鳄梨处理外,所有处理的根鲜重都较低。由此可见,香蕉、黑梅、巴西胡椒、墨西哥向日葵、迷迭香、鳄梨、薰衣草、柠檬草和薄荷等植物的化感提取物可能会影响紫荆草等问题杂草的萌发或生长。
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引用次数: 3
New evaluation of Alternaria brassicicola isolates against Striga hermonthica seeds emergence on maize in Kenya 肯尼亚玉米抗稻月曲菌种子羽化的新鉴定
Pub Date : 2021-06-01 DOI: 10.26655/JRWEEDSCI.2021.2.8
Reuben Abogno Ogada, E. M. Njeru, A. Omari, Johna Kibrono Birgen
Striga hermonthica (Del.) Benth is also known as witchweed, is a highly noxious parasite of cereal in Sub- Saharan Africa leading to enormous economic losses of above 7 billion US$ yearly. Some of the suggested control methods have been ineffective, therefore, the weed has continued to increase its host range and area under infestation. The objective of this study was to evaluate Alternaria brassicicola isolates against Striga hermonthica seeds' emergence on maize. A greenhouse experiment was performed at KALRO/CYMMTRY Kibos station, Kisumu, Kenya during April to August, 2019. The experiment included local maize landrace known as “Rachar”, Striga hermonthica seeds Kibos ecotype and four strains of Alternaria brassicicola. The treatments were; Soils (Treated) + two maize seed + S. hermonthica seeds + each isolate (LM017, LM013, LM019a and NY021) independently and soils (Treated) + three maize seeds + S. hermonthica seeds only (Control). A. brassicicola isolates LM019a, LM013 and LM017 inhibited Striga seeds emergence by 78.9%, 57.3% and 29.1% respectively. However, isolate NY021 enhanced Striga seeds emergence by 30.4% over the control. This study, established that some strains of A. brassicicola can inhibit the emergence of Striga weed. Therefore, the fungus could be used as a possible bio-agent in the production of mycoherbicide against Striga weed.
Striga hermonthica (Del)Benth也被称为独脚金,是撒哈拉以南非洲谷物中的一种剧毒寄生虫,每年造成超过70亿美元的巨大经济损失。一些建议的控制方法无效,因此,杂草继续增加其寄主范围和侵染面积。本研究的目的是评价分离株对玉米赤月曲菌种子萌发的抑制作用。2019年4月至8月,在肯尼亚基苏木的KALRO/CYMMTRY Kibos站进行了温室试验。该试验包括当地的地方玉米品种“Rachar”、稻月Striga种子Kibos生态型和4个油菜互交菌株系。治疗方法是;土壤(处理过的)+两粒玉米种子+月月花种子+独立分离物(LM017、LM013、LM019a和NY021),土壤(处理过的)+三粒玉米种子+月月花种子(对照)。菌株LM019a、LM013和LM017对striiga种子出苗的抑制作用分别为78.9%、57.3%和29.1%。与对照相比,分离株NY021的出苗率提高了30.4%。本研究证实,某些菌株的芸苔菌可以抑制striiga杂草的出现。因此,该真菌可作为一种生物制剂用于生产抗曲带菌杀菌剂。
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引用次数: 1
Prevalence and socio-economic impact of Striga (Striga hermonthica) in sorghum producing areas of east and west Hararghe zones, Ethiopia 衣索比亚哈拉尔河东部和西部高粱产区斯特里加(Striga hermonthica)的流行和社会经济影响
Pub Date : 2021-06-01 DOI: 10.26655/jrweedsci.2021.3.7
Lemma Degebasa Olkeba, T. Wondimu, Z. Bekeko, K. Belete
The field survey focused on potential sorghum-producing east and west Hararghe zones in six districts comprising 18 villages in the 2019 cropping season. Overall, 720 growers were nominated for considering the socio-economic impact. The representative growers were purposively selected. Data collected were Striga counts per m2 and per plant, time of Striga introduction, awareness and impression of farmers, prevalence, management used, severity, and collective actions to manage Striga. Both genders were included. Data were collected from interviews and analyzed. Statistical software (SPSS) summarizes the information. Results showed two Striga species, Striga hermonthica and Striga asiatica were observed and recorded. Striga hermonthica is more distributed than Striga asiatica in all the study locations and its occurrence diverse between locations. The maximum levels of Striga occurrence was perceived at Kile-besidimo (92%), Edobaso (85%), Kufakas (82%), Kotora (80%), Homacho Riana(78%), Bal’ina arba (74%), Dire gudina (72%), Bishan babile,(66%), Qufa (65%), Oda Anesso (48%), Ijakechu (45%), Umer kulle (40%), Homacho Eba (38%) and Tofik (35%). In contradict less number of Striga prevalence was perceived at Bareda (29%), Haro Adii (27%), Jiru gemachu (25%), and Homacho dayo (23%) striga per m2 in assessed fields. The striga count per plant was also recorded from each site. Sorghum yield loss due to Striga across surveyed villages was estimated to range between 0% and 80%. The management practices focused on improving the shortages suggested for controlling Striga in the districts.
实地调查的重点是2019年种植季哈拉尔河东部和西部6个地区18个村庄的高粱生产潜力。考虑到社会经济影响,总共有720名种植者被提名。有目的地选择具有代表性的种植户。收集的数据包括每平方米和每株斯特里加菌数、引进斯特里加菌的时间、农民对斯特里加菌的认识和印象、流行情况、使用的管理、严重程度和管理斯特里加菌的集体行动。男女都包括在内。从访谈中收集数据并进行分析。统计软件(SPSS)总结了这些信息。结果:观察到并记录到2种Striga,即她月Striga和亚洲Striga。在所有的研究地点,她月Striga的分布都比亚洲Striga多,而且在不同地点的分布也不同。在Kile-besidimo(92%)、Edobaso(85%)、Kufakas(82%)、Kotora(80%)、Homacho Riana(78%)、Bal 'ina arba(74%)、Dire gudina(72%)、Bishan babile(66%)、Qufa(65%)、Oda Anesso(48%)、Ijakechu(45%)、Umer kulle(40%)、Homacho Eba(38%)和Tofik(35%),认为斯特里加病毒的最高发生率为:相反,在被评估的农田中,Bareda(29%)、Haro Adii(27%)、Jiru gemachu(25%)和Homacho dayo(23%)的Striga患病率较低。每个站点还记录了每株株的striga计数。据估计,受调查村庄因斯特里加造成的高粱产量损失在0%至80%之间。管理实践的重点是改善为控制地区的斯特里加而建议的短缺。
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引用次数: 0
Effect of weed management strategies on the yield performance of aromatic rice in Boro season 不同杂草管理策略对Boro季香稻产量的影响
Pub Date : 2021-03-01 DOI: 10.26655/jrweedsci.2021.1.1
S. Sarker, S. Paul, U. Sarker, K. Saha, M. A. R. Sarkar
The experiment was conducted at the Agronomy Field Laboratory, Bangladesh Agricultural University, Mymensingh during November 2016 to May 2017 to study the effect of weed management strategies on the yield of aromatic fine rice in Boro season. The experiment comprised three aromatic fine rice varieties viz. BRRI dhan50, BRRI dhan63 and Basmati, and five weed management strategies viz. weedy check (no weeding), two hand weeding at 15 and 30 days after transplanting (DAT), pre-emergence herbicide, Panida 33 EC @ 2.5 l ha-1 + one hand weeding at 30 DAT, post emergence herbicide, Granite 240 SC @ 95 ml ha-1 + one hand weeding at 30 DAT, pre-emergence herbicide, Panida 33 EC @ 2.5 l ha-1 + post emergence herbicide, Granite 240 SC @ 95 ml ha-1. The experiment was laid out in a randomized complete block design with three replications. BRRI dhan50 produced the highest number of effective tillers hill-1 (8.87), number of grains panicle-1 (95.51 grain) and 1000-grain weight (21.45 g) and straw (5.59 t ha-1) yields compared to BRRI dhan63 and Basmati. The highest grain yield (4.31 t ha-1) was produced in BRRI dhan50 which was at par with BRRI dhan63 and the lowest one (3.21 t ha-1) was recoded in Basmati. In case of weed management, the highest number of effective tillers hill-1 (9.62), 1000-grain weight (21.70 g), grain (5.22 t ha-1) and straw (5.64 t ha-1) yields were obtained in pre-emergence herbicide + post emergence herbicide compared to other treatments. In interaction, the highest grain yield (5.54 t ha-1) was obtained in BRRI dhan50 with pre-emergence herbicide + post emergence herbicide, which was statistically identical to BRRI dhan63 with pre-emergence herbicide + post emergence herbicide. Therefore, BRRI dhan50 or BRRI dhan63 can be cultivated with pre-emergence herbicide + post emergence herbicide in Boro season for appreciable grain yields.
本试验于2016年11月至2017年5月在孟加拉国农业大学Mymensingh农学田间实验室进行,旨在研究Boro季节杂草管理策略对芳香细米产量的影响。实验由三个芳香优良水稻品种即BRRI dhan50十分,BRRI dhan63十分和印度香米,和五个杂草管理策略viz.杂草丛生的检查(没有除草),两个手除草15岁和移植后30天(DAT) pre-emergence除草剂,Panida 33 EC @ 2.5 l农业+ 30 DAT一方面除草,出现除草剂,花岗岩240 SC @ 95毫升农业+ 30 DAT一方面除草,pre-emergence除草剂,Panida 33 EC @ 2.5 l农业+帖子出现除草剂,花岗岩240 SC @ 95毫升ha-1。试验采用完全随机区组设计,设3个重复。与BRRI dhan63和Basmati相比,BRRI dhan50的有效分蘖数(8.87)、粒数(95.51)、千粒重(21.45 g)和秸秆(5.59 t hm -1)产量最高。BRRI dhan50的产量最高,为4.31 t ha-1,与BRRI dhan63相当,而Basmati的产量最低,为3.21 t ha-1。在杂草管理情况下,出苗期前除草剂+出苗期后除草剂处理的有效分蘖数、千粒重(21.70 g)、籽粒产量(5.22 t hm -1)和秸秆产量(5.64 t hm -1)高于其他处理。在互作中,出苗期前除草剂+出苗期后除草剂BRRI dhan50的籽粒产量最高(5.54 t hm -1),与出苗期前除草剂+出苗期后除草剂BRRI dhan63具有统计学上的一致性。因此,BRRI dhan50或BRRI dhan63可以在Boro季节使用出苗期前除草剂+出苗期后除草剂进行栽培,获得可观的产量。
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引用次数: 1
Soil seed bank and mapping Chromolaena odorata an invasive weed in agro-ecosystems of Serengeti district, Tanzania 坦桑尼亚塞伦盖蒂地区农业生态系统中入侵杂草臭草的土壤种子库与制图
Pub Date : 2020-09-12 DOI: 10.26655/jrweedsci.2021.1.3
G. Bulenga, K. P. Sibuga, E. Mtengeti
The spread of Chromolaena odorata in some parts of Serengeti district has stimulated interest in the prediction of their soil seed bank and mapping its distribution. Thirty clusters from each agro-ecosystem namely fallow land, grazing land, and cultivated land were sampled for soil seed bank assessment. Each cluster was randomly demarcated into five 20 m2 plots. Soils were collected from 0-5 cm, 5-10 cm, and 10-15 cm depths using a 10 cm diameter and 5 cm length cylinder. Coordinates were taken using a hand held GPS (Map76 cx) along transects. Each soil sample was soaked for a minimum of 30 minutes in a solution of sodium hexametaphosphate (50 g/L) and sodium bicarbonate (25 g/L) and poured over a set of three different sieve sizes to remove debris. The results were converted to number of weed seeds per square meter, and statistically analysed using R software version 3.5.1. Quantum GIS (1.8.0) was used to map the distribution of the weed. The soil seed bank in all agro-ecosystems decreased significantly (p <0.05) from 0-5 cm to 10-15 cm soil depths. Mean weed seed densities varied depending on land use but significantly highest in fallow land followed by cultivated land (p <0.05). In cultivated land the top soil had more than twice Siam weed seed densities (1254 seeds/m2) than in the bottom soil layers (597 seeds/m2). The Siam weed was found to have ability to thrive in a wide variety of soils, hence increase its invasion success. Distribution maps of C. odorata in Serengeti district and surrounding areas provide insight on the aggressiveness of the weed according to its spread and infestation levels. Future research work should be carried out to study fallow age in relation to soil seed bank of Chromolaena odorata and physio-chemical properties of the soil and their influence on distribution of the weed.
在塞伦盖蒂地区的一些地区,番茄色霉的传播激发了人们对土壤种子库预测和绘制其分布图的兴趣。从每个农业生态系统中选取30个集群,即休耕地、放牧地和耕地,进行土壤种子库评估。每个集群随机划分为5个20平方米的地块。使用直径为10 cm、长度为5 cm的圆柱体从0-5 cm、5-10 cm和10-15 cm深度采集土壤。使用手持式GPS (Map76 cx)沿横断面获取坐标。每个土壤样品在六偏磷酸钠(50 g/L)和碳酸氢钠(25 g/L)的溶液中浸泡至少30分钟,然后倒在一组三种不同尺寸的筛子上以去除碎片。将结果换算为每平方米杂草种子数,采用R软件3.5.1进行统计分析。采用Quantum GIS(1.8.0)软件绘制杂草分布图。各农业生态系统土壤种子库在0 ~ 5 cm土层至10 ~ 15 cm土层显著降低(p <0.05)。不同土地类型的杂草种子密度差异显著,但休耕地最高,其次为耕地(p <0.05)。耕地表层暹罗杂草种子密度(1254粒/m2)是底层暹罗杂草种子密度(597粒/m2)的两倍多。暹罗杂草被发现有能力在各种各样的土壤中茁壮成长,因此增加了其入侵的成功。塞伦盖蒂地区及周边地区的臭草分布图,根据其蔓延和侵害程度,提供了对臭草侵袭性的深入了解。今后应进一步研究休耕年限与臭草土壤种子库的关系、土壤理化性质及其对臭草分布的影响。
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引用次数: 0
Performance of different herbicides and their combinations on weed control, growth and yield parameters of onion ( Allium cepa L.) 不同除草剂及其组合对洋葱杂草控制、生长及产量参数的影响
Pub Date : 2020-09-01 DOI: 10.26655/JRWEEDSCI.2020.3.7
S. El-Sagheer, Taha Yossef Hilal, S. Temerak, Sherif Abou
Two experiments were carried out to study the efficacy of herbicides with different rates for controlling broadleaf and narrow leaves weeds, growth and yield parameters of onion. The treatments of herbicides applied include the single of active ingredient and their combinations. The single of active ingredient such as Oxyfluorfen at the dose of 270; 180; and 90 g a.i./fed, Clethodim at the dose of 93.75; 62.5; and 31.25 g a.i./fed. The combination used the Oxyfluorfen + Clethodim such as 270+93.75; 180+62.5; and 90+31.25 g a.i./fed. Results showed that Weed control efficiency (WCE) for broadleaves species the maximum percent was recorded for Oxyfluorfen (270 g a.i./fed). In controlling narrow leaves weed species, the maximum percent was recorded in Clethodim (93.75 g a.i./fed). While controlling broadleaf and narrow leaves weeds, the maximum percent was recorded in Oxyfluorfen + Clethodim (270+93.75 g a.i./fed). Growth, and yield parameters as plant height, bulb weight and bulb diameters were recorded maximum in Oxyfluorfen + Clethodim (270+93.75 g a.i./fed) compared to rest for the treatments. We suggest that the best treatment was Oxyfluorfen + Clethodim (270+93.75 g a.i./fed) for controlling all species of weeds in the onion plant.
通过2个试验,研究了不同用量除草剂对阔叶和窄叶杂草的防治效果以及洋葱的生长和产量参数。除草剂的施用处理包括有效成分的单一施用和有效成分的组合施用。氟氧芬等有效成分的单一剂量为270;180;90 g a.i./ d,以93.75剂量给药;62.5;31.25 g a.i./fed。组合使用氟氧芬+氯虫灵如270+93.75;180 + 62.5;90+31.25 g a.i./fed。结果表明,氟氧芬(270 g a.i./饲料)对阔叶植物的除草效率最高;在控制窄叶杂草种类方面,竹竹的防效率最高(93.75 g a.i./饲料)。在阔叶杂草和窄叶杂草的防治中,氟虫芬+杀虫灵的防效率最高(270+93.75 g a.i./饲料)。与其他处理相比,氟氧芬+乳香(270+93.75 g a.i./饲料)处理的生长和产量参数(如株高、鳞茎质量和鳞茎直径)最高。结果表明,以氟氧芬+杀虫灵(270+93.75 g a.i./投喂)处理对洋葱所有杂草均有较好的防治效果。
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引用次数: 1
Weeds, Pollinators, and Parasitoids - Using Weeds for Insect Manipulation in Agriculture 杂草、传粉媒介和拟寄生物。在农业中利用杂草控制昆虫
Pub Date : 2020-09-01 DOI: 10.26655/JRWEEDSCI.2020.3.9
Blaire Kleiman, A. Primoli, S. Koptur, K. Jayachandran
The use of weeds as insectary plants is an emerging management tactic by agroecologists to sustain beneficial insect species. Fallow lands have always been used by insects, and are an important part of their diet in fragmented ecosystems. Weeds provide nectar and floral resources to beneficial insects, and provide resources to keep those insects within a field in between flowering events. Using weeds as a tool in agricultural production reliant on pollination allows farmers to increase yield, end herbicide use, and increase biodiversity of both plants and insects. Native weeds expand the range of native insects from natural areas into agroecosystems, supporting insects that buffer against lapses in pollination by agricultural honey bees. Weeds also support parasitoid and predatory insects by providing nectar and pollen to adults, as well as alternative prey. This review examines the plant-insect ecological interactions supported by weeds left within a farm, and their potential role in supporting pollinators and parasitoids.
利用杂草作为食虫植物是农业生态学家用来维持有益昆虫物种的一种新兴管理策略。休耕土地一直是昆虫的栖息地,在支离破碎的生态系统中,休耕土地是昆虫饮食的重要组成部分。杂草为益虫提供花蜜和花卉资源,并在花期之间提供资源将这些昆虫留在田间。在依赖授粉的农业生产中,将杂草作为一种工具,使农民能够提高产量,结束除草剂的使用,并增加植物和昆虫的生物多样性。原生杂草将原生昆虫的范围从自然区域扩展到农业生态系统,支持昆虫缓冲农业蜜蜂授粉的失误。杂草还通过为成虫提供花蜜和花粉以及其他猎物来支持寄生性和掠食性昆虫。本文综述了农田杂草支持的植物-昆虫生态相互作用及其在支持传粉媒介和寄生蜂中的潜在作用。
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引用次数: 6
Chemical and molecular characteristics of winter oilseed rape (Brassica napus L.) volunteers from the soil seed bank 土壤种子库中冬季油菜(Brassica napus L.)志愿者的化学和分子特征
Pub Date : 2020-06-01 DOI: 10.26655/JRWEEDSCI.2020.3.10
A. Liersch, J. Bocianowski, W. Popławska, F. Wielebski, I. Bartkowiak-Broda
Oilseed rape (OSR) has one of the highest potential for gene flow through seed and pollen. Volunteer plants are one of the most important sources of contamination of OSR crop of different quality cultivars. The aim of this study was to estimate the abundance of soil seed bank after the harvest of high erucic (HE) cultivar Maplus and to determine biochemical and molecular characteristics of OSR volunteers. The investigation comprised volunteers of oilseed rape obtained from the soil seed bank in two localities in Poland: Dlon and Zielecin (Greater Poland Voivodeship). The seeds of volunteers and reference cultivars were analyzed using biochemical (erucic acid and glucosinolates [GLS]) and molecular biology methods. In seeds of volunteers, erucic acid content ranged from 0 to 57.4% of all fatty acids and glucosinolate content ranged from 5.2 to 105.0 µmol g−1 of seeds. In sowing seeds of the cultivar Maplus, the erucic acid content was 57% and total glucosinolates were 12.6 µmol g−1 of seeds. RAPD markers were used to determine genetic similarity coefficient and relationship between polymorphic RAPD markers and erucic acid and total GLS content. Twenty RAPD markers showed statistically significant association with erucic acid and GLS content in seeds. Volunteers originating from the investigated soil seed bank were characterized by different content of erucic acid and GLS in seeds typical for different types of oilseed rape cultivars: double low (00, canola type), cultivars with zero erucic acid and high GLS content (0HG) and traditional cultivars with high erucic acid and high GLS content (HEHG). The results of this study, especially the presence in soil seed bank the seeds of very old cultivars, confirm that seeds of rapeseed of different origin may persist for a long time at the stage of secondary dormancy.
油菜(OSR)是通过种子和花粉进行基因流动的潜力最大的植物之一。志愿植物是不同品质品种OSR作物污染的重要来源之一。本研究的目的是估计高芥子(high erucic, Maplus)品种收获后土壤种子库的丰度,并确定OSR志愿者的生化和分子特征。调查人员包括从波兰两个地方的土壤种子库获得的油菜志愿者:德隆和齐耶林(大波兰省)。采用生化(芥酸和硫代葡萄糖苷[GLS])和分子生物学方法对志愿者和参考品种的种子进行分析。在志愿者的种子中,芥酸含量在所有脂肪酸中占0 ~ 57.4%,硫代葡萄糖苷含量在种子中占5.2 ~ 105.0µmol g−1。在播种Maplus种子时,芥子酸含量为57%,总硫代葡萄糖苷含量为12.6µmol g−1。利用RAPD标记测定遗传相似系数及多态性RAPD标记与芥酸和总GLS含量的关系。20个RAPD标记与种子中芥酸和GLS含量呈显著相关。来自被调查土壤种子库的志愿者种子中芥酸和GLS含量不同,具有不同类型油菜品种的典型特征:双低(00,油菜型)、零芥酸和高GLS含量(0HG)的品种和高芥酸和高GLS含量(HEHG)的传统品种。本研究的结果,特别是在土壤种子库中存在着非常古老的品种的种子,证实了不同来源的油菜籽在次生休眠阶段可能会持续很长时间。
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引用次数: 0
Seed germination and seedling growth of some crops and weed seeds under different environmental conditions 不同环境条件下某些作物和杂草种子的发芽和幼苗生长
Pub Date : 2020-06-01 DOI: 10.26655/JRWEEDSCI.2020.3.8
Mallik Baby Babita Das, B. Acharya, M. Saquib, M. Chettri
Seed germination of some crops (Brassica campestris and Triticum aestivum) and weed seeds (Ageratum conyzoides, Bidens pilosa, Cyperus rotundus and Galinsoga parviflora) was studied under different environmental conditions like moisture (concentrations 3, 6, 9, 12, 15ml), temperature (5, 10, 15, 20 and 25˚C), pH (value 4, 5, 6, 7, 8 and 9) and light (normal, red, yellow, blue, green and dark color). For the crops too much lower or higher moisture was not favorable for germination and growth. Experiments under different moisture conditions showed that C. rotundus and G. parviflora do not require more moisture to germinate and grow. Seed germination of both crops enhanced insignificantly at 15 to 20˚C treatments. Seed germination of all weed seeds was insignificantly high at 10 to 15˚C. The percentage of seed germination of both crops increased significantly in normal and green light. Seed germination of A. conyzoides, C. rotundus and G. parviflora was insignificantly different in normal, red, yellow and green light. Germination of all weed seeds was completely inhibited by blue and dark light. The shoot and root length of weed A. conyzoides was found to be significantly high (P=0.05) in yellow light treatment, but in B. pilosa, it was high in red and green light. Similarly in C. rotundus, shoot and root length were high in normal light but in of G. parviflora it was high in green light treatment. Seed germination and seedling growth were higher in pH5-7 in most cases.
研究了几种作物(芸苔、小麦)和杂草(Ageratum conyzoides、Bidens pilosa、Cyperus rotundus和Galinsoga parviflora)种子在不同湿度(浓度3、6、9、12、15ml)、温度(5、10、15、20、25℃)、pH(值4、5、6、7、8、9)和光照(正常、红、黄、蓝、绿、暗色)条件下的萌发情况。过高或过低的水分都不利于作物的发芽和生长。不同水分条件下的试验结果表明,圆圆草和小花草的发芽和生长不需要更多的水分。在15 ~ 20℃处理下,两种作物的种子萌发均无显著提高。10 ~ 15℃时,所有杂草种子的萌发率均不显著升高。两种作物的种子发芽率在正常光和绿光下均显著提高。在正常光、红光、黄光和绿光下,圆锥花、圆形花和小花的种子萌发率差异不显著。蓝光和暗光对所有杂草种子的萌发均有抑制作用。黄光处理下,金合欢的茎长和根长显著高于黄光处理(P=0.05),而红、绿光处理下,金合欢的茎长和根长高于黄光处理(P=0.05)。在正常光照下,圆圆藤的茎长和根长都很高,而在绿光下,小叶藤的茎长和根长都很高。在pH5-7的条件下,大多数情况下种子萌发率和幼苗生长率较高。
{"title":"Seed germination and seedling growth of some crops and weed seeds under different environmental conditions","authors":"Mallik Baby Babita Das, B. Acharya, M. Saquib, M. Chettri","doi":"10.26655/JRWEEDSCI.2020.3.8","DOIUrl":"https://doi.org/10.26655/JRWEEDSCI.2020.3.8","url":null,"abstract":"Seed germination of some crops (Brassica campestris and Triticum aestivum) and weed seeds (Ageratum conyzoides, Bidens pilosa, Cyperus rotundus and Galinsoga parviflora) was studied under different environmental conditions like moisture (concentrations 3, 6, 9, 12, 15ml), temperature (5, 10, 15, 20 and 25˚C), pH (value 4, 5, 6, 7, 8 and 9) and light (normal, red, yellow, blue, green and dark color). For the crops too much lower or higher moisture was not favorable for germination and growth. Experiments under different moisture conditions showed that C. rotundus and G. parviflora do not require more moisture to germinate and grow. Seed germination of both crops enhanced insignificantly at 15 to 20˚C treatments. Seed germination of all weed seeds was insignificantly high at 10 to 15˚C. The percentage of seed germination of both crops increased significantly in normal and green light. Seed germination of A. conyzoides, C. rotundus and G. parviflora was insignificantly different in normal, red, yellow and green light. Germination of all weed seeds was completely inhibited by blue and dark light. The shoot and root length of weed A. conyzoides was found to be significantly high (P=0.05) in yellow light treatment, but in B. pilosa, it was high in red and green light. Similarly in C. rotundus, shoot and root length were high in normal light but in of G. parviflora it was high in green light treatment. Seed germination and seedling growth were higher in pH5-7 in most cases.","PeriodicalId":16980,"journal":{"name":"Journal of Research in Weed Science","volume":"23 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81643983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
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Journal of Research in Weed Science
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