Pub Date : 2021-11-01DOI: 10.18173/2354-1059.2021-0062
Tuan Trieu Anh, Viet Nguyen Xuan, Binh Thai Thanh
This study was conducted to evaluate the effects of farming methods and density on the survival rate, growth, yeild and economic efficency of commercial otter clam in the sea waters of Van Don, Quang Ninh. The otter clams with an initial length of 18,36 mm and an average body weight of 4,12 g were used in this study. Two farming methods were arranged: cage culture (basket) hanging on the raft (including experiments TN1, TN2, TN3 and TN4) and cage culture placed on tidal flats (including experiments TN5, TN6, TN7 and TN8). For each culture, there were 4 formulas for stocking density of 25 individualscage (100 individualsm2) (TN1, TN5), 35 individualscage (140 individualsm2) (TN2, TN6); 45 individualscage (180 individualsm2) (TN3, TN7); and 55 individualscage (220 individualsm2) (TN4, TN8). Each treatment was triplicate and lasted for 12 months, the results showed that the density and farming methods had different effects on growth, survival rate and yield of commercial otter clam. The weight of the commercial otter clam ranged from 28,76 to 41,74 gindividual, the length varied from 51,17 to 72,69 mmindividual, and the survival rate fluctuated from 57,4 to 78,3%. The highest growth was recorded in the TN1 with density of 25 individualscage, the difference between treatments was statistically significant (p≤ 0.05). The average yield of commercial otter clam increased with the increase of stocking density, ranging from 21,7 to 38,3 tonsha, the difference was statistically significant (p≤ 0,05). However, economic accounting showed that the economic efficiency was highest in TN2, the ottet clams were arranged in the cage hanging on rafts and stocking at a density of 35 individualscage (140 individualsm2). In this experiment, the average growth in weight was 38,91 gindividual and in length was 68,47 mmindividual, the survival rate was 76,4%, and the yield was 32,9 tonsha the economic profit reached 0,883 billion of Vietnam Dong and the profit rate reached 28,1, respectively. Based on these research results, it could be recommended to farmers to stock the otter clam with a density of 35 individualscage.
本研究旨在评价不同养殖方式和养殖密度对广宁文敦市商品水獭蚌成活率、生长、产量和经济效益的影响。本研究选用初始长度为18.36 mm，平均体重为4.12 g的水獭蛤。布置两种养殖方式:筏挂网箱(篮)养殖(包括试验TN1、TN2、TN3、TN4)和滩涂网箱养殖(包括试验TN5、TN6、TN7、TN8)。每种培养有4个放养密度公式:25只笼(100只m2) (TN1、TN5)、35只笼(140只m2) (TN2、TN6);45只笼(180只m2) (TN3, TN7);55只笼(220只m2) (TN4, TN8)。结果表明，密度和养殖方式对商品水獭蚌的生长、成活率和产量有不同程度的影响。商品水獭蚌体重为28.76 ~ 41.74 g个体，体长为51.17 ~ 72.69 mm个体，成活率为57.4% ~ 78.3%。以密度为25只/笼的TN1生长最高，处理间差异有统计学意义(p≤0.05)。商品水獭蚌平均产量随放养密度的增加而增加，在21.7 ~ 383 t / ha之间，差异有统计学意义(p≤0.05)。但经经济核算表明，TN2的经济效益最高，以35只/笼(140只/ m2)的密度挂筏放养。本试验平均增重38.91 g /单株，平均增长68 47 mm /单株，成活率76.4%，产量32.9吨/公顷，经济利润达0.8830亿越盾，利润率达28.1。根据以上研究结果，建议养殖户以35只/笼的密度放养水獭蚌。
{"title":"Combined effects of the farming methods and density on the survival rate, growth, yield, and economic efficiency of otter clam (Lutraria rhynchaena) in Van Don, Quang Ninh Province","authors":"Tuan Trieu Anh, Viet Nguyen Xuan, Binh Thai Thanh","doi":"10.18173/2354-1059.2021-0062","DOIUrl":"https://doi.org/10.18173/2354-1059.2021-0062","url":null,"abstract":"This study was conducted to evaluate the effects of farming methods and density on the survival rate, growth, yeild and economic efficency of commercial otter clam in the sea waters of Van Don, Quang Ninh. The otter clams with an initial length of 18,36 mm and an average body weight of 4,12 g were used in this study. Two farming methods were arranged: cage culture (basket) hanging on the raft (including experiments TN1, TN2, TN3 and TN4) and cage culture placed on tidal flats (including experiments TN5, TN6, TN7 and TN8). For each culture, there were 4 formulas for stocking density of 25 individualscage (100 individualsm2) (TN1, TN5), 35 individualscage (140 individualsm2) (TN2, TN6); 45 individualscage (180 individualsm2) (TN3, TN7); and 55 individualscage (220 individualsm2) (TN4, TN8). Each treatment was triplicate and lasted for 12 months, the results showed that the density and farming methods had different effects on growth, survival rate and yield of commercial otter clam. The weight of the commercial otter clam ranged from 28,76 to 41,74 gindividual, the length varied from 51,17 to 72,69 mmindividual, and the survival rate fluctuated from 57,4 to 78,3%. The highest growth was recorded in the TN1 with density of 25 individualscage, the difference between treatments was statistically significant (p≤ 0.05). The average yield of commercial otter clam increased with the increase of stocking density, ranging from 21,7 to 38,3 tonsha, the difference was statistically significant (p≤ 0,05). However, economic accounting showed that the economic efficiency was highest in TN2, the ottet clams were arranged in the cage hanging on rafts and stocking at a density of 35 individualscage (140 individualsm2). In this experiment, the average growth in weight was 38,91 gindividual and in length was 68,47 mmindividual, the survival rate was 76,4%, and the yield was 32,9 tonsha the economic profit reached 0,883 billion of Vietnam Dong and the profit rate reached 28,1, respectively. Based on these research results, it could be recommended to farmers to stock the otter clam with a density of 35 individualscage.","PeriodicalId":17007,"journal":{"name":"Journal of Science Natural Science","volume":"24 7","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91434874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-11-01DOI: 10.18173/2354-1059.2021-0083
Man Le Thi, Na Nguyen Quoc, H. Tran Thi Thanh, Hong La Viet, Bang Cao Phi
The HSP90 gene family has been shown to play an important role in the tolerance and development of plants. Papaya, which is a fruit crop with high nutritional value, is native to the tropics but now is widely cultivated in many subtropical regions of the world. Therefore, papaya plants have to face many environmental factors during their life. This study aims to identify and analyze the HSP90 gene family in papaya by bioinformatics method. A total of seven HSP90 genes have been identified in the genome of papaya (Carica papaya L.) by using the bioinformatic methods. The full-length genomic sequence of papaya HSP90 genes were ranging from 2650 to 8136 nucleotides, non continuous coding, with number of intro ranging from two to 19. The predicted protein sequences included from 348 to 796 amino acids, according to the molecular weight ranged from 39.92 to 90.61 kDa. Among seven CpHSP90, the two CpHSP90-1 and CpHSP90-4 were considered pseudogenes due to their small size. These proteins were acidic with a pI value ranging from 4.69 to 5.42, except CpHSP90-1 (pI 7.03). Based on the protein structure, subcellular localization and the phylogenic analysis, the papaya HSP90 were divided into two groups, I (cytoplasmic HSP90, four members) and II (organelle HSP90, three members). Analysis of transcriptomes showed that the papaya HSP90s were differentially expressed in different tissues at different development stages. In which, most of the papaya HSP90 is highly expressed in flower buds or in fruits at stage 2 or stage 3. CpHSP90-2 had the highest level of expression, followed by CpHSP90-5. In contrast, CpHSP90-1 was not expressed or very weakly expressed in these studied tissues. All of seven HSP90 genes of papaya were induced by freeze-thaw awakening treatment (in comparison with control treatment), among them, CpHSP90-1 was strongest induced by stress (12.13-folds), however, it was a pseudogene and had a very low level of basal expression. CpHSP90-2 had a high induction level (2.81- folds), and also had a high basal expression level compared to other HSP90 genes of papaya. The results of this work have an important significance and will serve as a base for the further research on gene cloning, functional analysis of HSP90 genes and breeding of papaya in response to environmental abiotic stresses and the development of this fruit crop.
{"title":"Identification and analysis of HSP90 genes in papaya (Carica papaya L.) by using bioinformatics method","authors":"Man Le Thi, Na Nguyen Quoc, H. Tran Thi Thanh, Hong La Viet, Bang Cao Phi","doi":"10.18173/2354-1059.2021-0083","DOIUrl":"https://doi.org/10.18173/2354-1059.2021-0083","url":null,"abstract":"The HSP90 gene family has been shown to play an important role in the tolerance and development of plants. Papaya, which is a fruit crop with high nutritional value, is native to the tropics but now is widely cultivated in many subtropical regions of the world. Therefore, papaya plants have to face many environmental factors during their life. This study aims to identify and analyze the HSP90 gene family in papaya by bioinformatics method. A total of seven HSP90 genes have been identified in the genome of papaya (Carica papaya L.) by using the bioinformatic methods. The full-length genomic sequence of papaya HSP90 genes were ranging from 2650 to 8136 nucleotides, non continuous coding, with number of intro ranging from two to 19. The predicted protein sequences included from 348 to 796 amino acids, according to the molecular weight ranged from 39.92 to 90.61 kDa. Among seven CpHSP90, the two CpHSP90-1 and CpHSP90-4 were considered pseudogenes due to their small size. These proteins were acidic with a pI value ranging from 4.69 to 5.42, except CpHSP90-1 (pI 7.03). Based on the protein structure, subcellular localization and the phylogenic analysis, the papaya HSP90 were divided into two groups, I (cytoplasmic HSP90, four members) and II (organelle HSP90, three members). Analysis of transcriptomes showed that the papaya HSP90s were differentially expressed in different tissues at different development stages. In which, most of the papaya HSP90 is highly expressed in flower buds or in fruits at stage 2 or stage 3. CpHSP90-2 had the highest level of expression, followed by CpHSP90-5. In contrast, CpHSP90-1 was not expressed or very weakly expressed in these studied tissues. All of seven HSP90 genes of papaya were induced by freeze-thaw awakening treatment (in comparison with control treatment), among them, CpHSP90-1 was strongest induced by stress (12.13-folds), however, it was a pseudogene and had a very low level of basal expression. CpHSP90-2 had a high induction level (2.81- folds), and also had a high basal expression level compared to other HSP90 genes of papaya. The results of this work have an important significance and will serve as a base for the further research on gene cloning, functional analysis of HSP90 genes and breeding of papaya in response to environmental abiotic stresses and the development of this fruit crop.","PeriodicalId":17007,"journal":{"name":"Journal of Science Natural Science","volume":"50 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90049886","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-11-01DOI: 10.18173/2354-1059.2021-0064
Hong Bui Minh, Tung Doan Manh
The study aimed to determine the species composition of beetles and their distribution in 3 habitats in Dong Son - Ky Thuong Nature Reserve, Quang Ninh. Direct investigation method of beetles at 3 habitats have been used. The results showed that there were 59 species of beetles belonging to 49 varieties and 18 families. The Scarabaeidae family and the Cerambycidae family with 12 species have the largest number of species. The Eulichadidae, Passalidae, Languriidae, Dytiscidae, Hydrophilidae, Lampyridae, Cleridae and Carabidae families have the lowest number of species with 1 species each. The primary forest habitat has the highest number of species with 35 species, accounting for 59.32% of the total species. The shrub grassland habitat has the least species with only 14 species, accounting for 23.73%. The number of species samples of beetles collected with light traps at night was11 species higher than that collected with racquets during the day.
{"title":"The initial research on species composition of insects (Coleoptera) at Dong Son - Ky Thuong Natural Reserve, Quang Ninh province","authors":"Hong Bui Minh, Tung Doan Manh","doi":"10.18173/2354-1059.2021-0064","DOIUrl":"https://doi.org/10.18173/2354-1059.2021-0064","url":null,"abstract":"The study aimed to determine the species composition of beetles and their distribution in 3 habitats in Dong Son - Ky Thuong Nature Reserve, Quang Ninh. Direct investigation method of beetles at 3 habitats have been used. The results showed that there were 59 species of beetles belonging to 49 varieties and 18 families. The Scarabaeidae family and the Cerambycidae family with 12 species have the largest number of species. The Eulichadidae, Passalidae, Languriidae, Dytiscidae, Hydrophilidae, Lampyridae, Cleridae and Carabidae families have the lowest number of species with 1 species each. The primary forest habitat has the highest number of species with 35 species, accounting for 59.32% of the total species. The shrub grassland habitat has the least species with only 14 species, accounting for 23.73%. The number of species samples of beetles collected with light traps at night was11 species higher than that collected with racquets during the day.","PeriodicalId":17007,"journal":{"name":"Journal of Science Natural Science","volume":"24 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72754153","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-11-01DOI: 10.18173/2354-1059.2021-0080
Trang Ha Thu, Huong Vuong Thi Quynh, Hoa Dieu Thi Mai, Sen Dao Thi, Dung Vu Thi, Quyen Nguyen Van
The Khang Dan 18 is one of the rice cultivars widely cultivated in Vietnam, which is threatened by saline intrusion. This study is conducted to establish an in vitro culture system for evaluate salt tolerance of the rice cultivar Khang Dan 18. First, different culture conditions, including plant growth regulator concentration, culture period, light regime, were optimized to induce and maintain callus in vitro. Culture media supplemented with 10 - 40 µM 2,4- Dichlorophenoxyacetic acid (2,4-D) are suitable for inducing callus. Different culture periods (1, 2 and 4 weeks) significantly affected the callus morphology and nature. The 10-hour light photoperiod is the most effective condition for callus induction and multiplication. Callus cultured in the medium supplemented exclusively with 2,4-D showed higher quality compared to those in media supplemented with 1-Naphthylacetic acid (NAA) and 2,4-D. Different photoperiods also affected callus differentiation in terms of rootshoot induction. The established culture system was then employed to evaluate salt tolerance of the callus. The growth of callus was significantly decreased when the concentrations of NaCl increased (3, 6 and 9‰, P = 0.05), especially at the concentration of 9‰ NaCl caused a strong decrease in callus weight and an obvious change in callus morphology. This culture system may be applied for in vitro salt stress studies on the cultivar Khang Dan 18 and similar rice cultivars.
{"title":"Establishment of in vitro callus induction and multiplication for studying salt stress in the rice cultivar Khang Dan 18","authors":"Trang Ha Thu, Huong Vuong Thi Quynh, Hoa Dieu Thi Mai, Sen Dao Thi, Dung Vu Thi, Quyen Nguyen Van","doi":"10.18173/2354-1059.2021-0080","DOIUrl":"https://doi.org/10.18173/2354-1059.2021-0080","url":null,"abstract":"The Khang Dan 18 is one of the rice cultivars widely cultivated in Vietnam, which is threatened by saline intrusion. This study is conducted to establish an in vitro culture system for evaluate salt tolerance of the rice cultivar Khang Dan 18. First, different culture conditions, including plant growth regulator concentration, culture period, light regime, were optimized to induce and maintain callus in vitro. Culture media supplemented with 10 - 40 µM 2,4- Dichlorophenoxyacetic acid (2,4-D) are suitable for inducing callus. Different culture periods (1, 2 and 4 weeks) significantly affected the callus morphology and nature. The 10-hour light photoperiod is the most effective condition for callus induction and multiplication. Callus cultured in the medium supplemented exclusively with 2,4-D showed higher quality compared to those in media supplemented with 1-Naphthylacetic acid (NAA) and 2,4-D. Different photoperiods also affected callus differentiation in terms of rootshoot induction. The established culture system was then employed to evaluate salt tolerance of the callus. The growth of callus was significantly decreased when the concentrations of NaCl increased (3, 6 and 9‰, P = 0.05), especially at the concentration of 9‰ NaCl caused a strong decrease in callus weight and an obvious change in callus morphology. This culture system may be applied for in vitro salt stress studies on the cultivar Khang Dan 18 and similar rice cultivars.","PeriodicalId":17007,"journal":{"name":"Journal of Science Natural Science","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83014427","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-11-01DOI: 10.18173/2354-1059.2021-0066
Son Doan Hoang, Hai Do Van, Bach Tran The, Chen Cheng Wei, Duong La Anh, Hieu Trinh Van, Nhat Ha Huy, Tung Ngo Van
The genus Amblovenatum J.P. Roux is a small genus in the Thelypteridaceae. Previously, the genus Amblovenatum was named Amphineuron (Holttum, 1971). In 2007, David Middleton argued for the homonyms between the genus Ampineuron (Thelypteridaceae) and the genus Amphineurion (Apocynaceae). By 2009, the name Amblovenatum was used by J.P. Roux to replace the name Amphineuron. The genus Amblovenatum J.P. Roux has outstanding features in fronds, laminae, pinnae, lobes, segments, veinlets, sori, indusia, sporangia. Species of the genus Amblovanatum are distributed from East Africa to Polynesia; India to Northeast Australia; the remainder in Malesia and the Pacific, especially in New Guinea, and has been reported to be distributed in Vietnam. The study added information and explanations on the genus name change and updated the new name of the genus Amblovenatum. Description the morphological characteristics of the genus Amblovenatum, distribution, ecological biology, type; species description Amblovenatum terminans (Hook.) J.P. Roux includes scientific name, Vietnamese name, distribution, ecology, type, examined specimens.
{"title":"Update name of Amblovenatum J.P.Roux genus (Thelypteridaceae) and received new distribution of Amblovetatum terminans in Vietnam","authors":"Son Doan Hoang, Hai Do Van, Bach Tran The, Chen Cheng Wei, Duong La Anh, Hieu Trinh Van, Nhat Ha Huy, Tung Ngo Van","doi":"10.18173/2354-1059.2021-0066","DOIUrl":"https://doi.org/10.18173/2354-1059.2021-0066","url":null,"abstract":"The genus Amblovenatum J.P. Roux is a small genus in the Thelypteridaceae. Previously, the genus Amblovenatum was named Amphineuron (Holttum, 1971). In 2007, David Middleton argued for the homonyms between the genus Ampineuron (Thelypteridaceae) and the genus Amphineurion (Apocynaceae). By 2009, the name Amblovenatum was used by J.P. Roux to replace the name Amphineuron. The genus Amblovenatum J.P. Roux has outstanding features in fronds, laminae, pinnae, lobes, segments, veinlets, sori, indusia, sporangia. Species of the genus Amblovanatum are distributed from East Africa to Polynesia; India to Northeast Australia; the remainder in Malesia and the Pacific, especially in New Guinea, and has been reported to be distributed in Vietnam. The study added information and explanations on the genus name change and updated the new name of the genus Amblovenatum. Description the morphological characteristics of the genus Amblovenatum, distribution, ecological biology, type; species description Amblovenatum terminans (Hook.) J.P. Roux includes scientific name, Vietnamese name, distribution, ecology, type, examined specimens.","PeriodicalId":17007,"journal":{"name":"Journal of Science Natural Science","volume":"64 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88941915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-11-01DOI: 10.18173/2354-1059.2021-0063
Thuy Le Thi, Uyen Duong Thi Thao, Hang Do Ngan, Thao Nguyen Phuong, Van Anh Pham Thi, Sen Dao Thi
This study was conducted to evaluate the effects of two forms of static hydroponics and dynamic hydroponics along with two nutrient solutions, BK-fast and Hydro Umat, on the growth and quality of celery plants. The results showed that "Thera" variety celery grown in the dynamic hydroponic system grew better than the static hydroponic growing in both studied solutions, with the plant height reaching 35.02 - 37.3 cm, 8.79 - 9.02 of leavesplant, the fresh weight of 32.14 - 39.92 g. Regarding the quality parameters, the dynamic hydroponic form increased the content of reducing sugars, total organic acids and nitrate while reducing the content of the mineral elements including potassium, iron and magnesium that accumulated in the celery plant. For two nutrient solutions, Hydro Umat solution helped plants grow better and increased nitrate accumulation compared to BK-fast solution. However, the results on parameters containing of number of leavesplant, reducing sugar content, the content of vitamin C and total organic acid were not statistical difference between celery plants nourished by these two solutions.
{"title":"Effects of hydroponic forms and nutrient solutions on some parameters related to yield and quality of celery (Apium graveolens)","authors":"Thuy Le Thi, Uyen Duong Thi Thao, Hang Do Ngan, Thao Nguyen Phuong, Van Anh Pham Thi, Sen Dao Thi","doi":"10.18173/2354-1059.2021-0063","DOIUrl":"https://doi.org/10.18173/2354-1059.2021-0063","url":null,"abstract":"This study was conducted to evaluate the effects of two forms of static hydroponics and dynamic hydroponics along with two nutrient solutions, BK-fast and Hydro Umat, on the growth and quality of celery plants. The results showed that \"Thera\" variety celery grown in the dynamic hydroponic system grew better than the static hydroponic growing in both studied solutions, with the plant height reaching 35.02 - 37.3 cm, 8.79 - 9.02 of leavesplant, the fresh weight of 32.14 - 39.92 g. Regarding the quality parameters, the dynamic hydroponic form increased the content of reducing sugars, total organic acids and nitrate while reducing the content of the mineral elements including potassium, iron and magnesium that accumulated in the celery plant. For two nutrient solutions, Hydro Umat solution helped plants grow better and increased nitrate accumulation compared to BK-fast solution. However, the results on parameters containing of number of leavesplant, reducing sugar content, the content of vitamin C and total organic acid were not statistical difference between celery plants nourished by these two solutions.","PeriodicalId":17007,"journal":{"name":"Journal of Science Natural Science","volume":"2 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78980936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-11-01DOI: 10.18173/2354-1059.2021-0076
Hoa Dieu Thi Mai, Huong Nguyen Thi Thuy
This study aims to evaluate the growth and dry matter accumulation of stevia grown in lighting conditions with different spectral ratios, thereby finding the most suitable lighting rate. Materials are stevia stalks cultured in shoot medium, including: MS base supplemented with 20 gLsucrose; 8 gLagar; 0.15 mgLBAP, 0.2 mgLNAA, cultured for 4 weeks under a LED system with different color light ratios, using the following formulas: 40% red + 20% blue + 40% orange (CT1), 20% red + 20% blue + 60% yellow (CT2), 72% red + 14% blue + 14% white (CT3) and 80% red + 20% blue (CT4) and the control formula was the formula of fluorescent lamp irradiation (DC). Formula CT4 gave a result of 100% shoot formation, the average height of shoots was 48.5 mmshoot and the shoots were the best in quality. Rooting medium included: MS basal supplemented with 15 g sucrose, 8 gLagar, 0.2 mgLNAA for 4 weeks. The results also showed that the CT4 lighting formula was the most suitable for the photosynthesis and growth of cultured stevia in the rooting stage. The seedlings from this lighting system when grown in the net house were able to survive and grow better than the plants from the other lighting conditions.
{"title":"Study on some physiology characteristics and growth of Stevia rebaudiana Bertoni in vitro in LED monochromatic light system and green house stage","authors":"Hoa Dieu Thi Mai, Huong Nguyen Thi Thuy","doi":"10.18173/2354-1059.2021-0076","DOIUrl":"https://doi.org/10.18173/2354-1059.2021-0076","url":null,"abstract":"This study aims to evaluate the growth and dry matter accumulation of stevia grown in lighting conditions with different spectral ratios, thereby finding the most suitable lighting rate. Materials are stevia stalks cultured in shoot medium, including: MS base supplemented with 20 gLsucrose; 8 gLagar; 0.15 mgLBAP, 0.2 mgLNAA, cultured for 4 weeks under a LED system with different color light ratios, using the following formulas: 40% red + 20% blue + 40% orange (CT1), 20% red + 20% blue + 60% yellow (CT2), 72% red + 14% blue + 14% white (CT3) and 80% red + 20% blue (CT4) and the control formula was the formula of fluorescent lamp irradiation (DC). Formula CT4 gave a result of 100% shoot formation, the average height of shoots was 48.5 mmshoot and the shoots were the best in quality. Rooting medium included: MS basal supplemented with 15 g sucrose, 8 gLagar, 0.2 mgLNAA for 4 weeks. The results also showed that the CT4 lighting formula was the most suitable for the photosynthesis and growth of cultured stevia in the rooting stage. The seedlings from this lighting system when grown in the net house were able to survive and grow better than the plants from the other lighting conditions.","PeriodicalId":17007,"journal":{"name":"Journal of Science Natural Science","volume":"26 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89954052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-11-01DOI: 10.18173/2354-1059.2021-0067
Thao Truong Thi Hieu, Hoang Ho Dac Thai, Hung Le Thai, Lien Nguyen Thi Hong
There were three vegetation types in blowing sand dunes of Mui Ne, Phan Thiet city, Binh Thuan province; native sand binding grasses type, mix species of native grasses and low shrub type and evergreen trees biome in coastal sand dune type. Group of dominant typical species were identified in each vegetation type in which three significant canopy layers were confirmed with evergreen trees biome in coastal sand dune type. The similarity among different vegetation types was evaluated, Sorenson’s index (S) showed that similarity of species in the vegetation types native sand binding grasses and mix species of natuve grasses and low shrub is 0.61, Shannon index (H’) for all three types is low but the highest come from evergreen trees biome in coastal sand dune type (H’ = 0.17 for trees and 0.06 for vines and shrub species. Results and lessons learned from this study are referred to conservation and ecological restoration activities.
{"title":"Vegetable structure in the Bay Sand Dune of Mui Ne, Phan Thiet city, Binh Thuan province","authors":"Thao Truong Thi Hieu, Hoang Ho Dac Thai, Hung Le Thai, Lien Nguyen Thi Hong","doi":"10.18173/2354-1059.2021-0067","DOIUrl":"https://doi.org/10.18173/2354-1059.2021-0067","url":null,"abstract":"There were three vegetation types in blowing sand dunes of Mui Ne, Phan Thiet city, Binh Thuan province; native sand binding grasses type, mix species of native grasses and low shrub type and evergreen trees biome in coastal sand dune type. Group of dominant typical species were identified in each vegetation type in which three significant canopy layers were confirmed with evergreen trees biome in coastal sand dune type. The similarity among different vegetation types was evaluated, Sorenson’s index (S) showed that similarity of species in the vegetation types native sand binding grasses and mix species of natuve grasses and low shrub is 0.61, Shannon index (H’) for all three types is low but the highest come from evergreen trees biome in coastal sand dune type (H’ = 0.17 for trees and 0.06 for vines and shrub species. Results and lessons learned from this study are referred to conservation and ecological restoration activities.","PeriodicalId":17007,"journal":{"name":"Journal of Science Natural Science","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82828856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-11-01DOI: 10.18173/2354-1059.2021-0068
Sengsoulichan Dethvongsa, Vu Nguyen Anh, Van Tran Khanh
The experiment screening of potential cassava varieties for good tolerance to artificial waterlogging was evaluated on 17 varieties of cassava in vitro at 3 months of age after planting in potting soil for 12 days through physiological parameters: number of leavesplant, number of yellow leaves, number of wilted plants and chlorophyll content. The results showed that cassava varieties had different tolerance to waterlogging: some varieties showed physiological symptoms only after 3 days of growth on waterlogging conditions such as the number of leavesplant decreasing to 83.93 %. 93.75 %, 93.81 %, 95.88 % and 96.48 % in cassava varieties C86, C23, Hanoi3, C84 and C31, respectively. After 6 days of waterlogging, the percentage of yellow leaves in some varieties of cassava had been increased, attained up to 40 % of the total leavesplant turned yellow such as C71 (44.68 %) or C13 (53.12 %) varieties while other cassava varieties wilted and die 100 % such as Hanoi3, C10, C13, C34, C42, C66, C71, C84 and C86; along with the symtoms of morphological and growth of cassava varieties on waterlogging conditions, the total of chlorophyll content also decreased. After 9 days of waterlogging, only C60 variety remained chlorophyll content with 77.38 % but in other cassava varieties, the chlorophyll content was not more than 50 %. We founded that the longer the duration of waterlogging, the more tolerant response of the studied cassava varieties was revealed. After 12 days of experiment, we initially determined that the C60 variety was the best flood tolerance among the 17 studied varieties and this was a good source of materials on selecting the variety of flood tolerant cassava.
{"title":"Screening of potential cassava varieties for good tolerance under waterlogging conditions","authors":"Sengsoulichan Dethvongsa, Vu Nguyen Anh, Van Tran Khanh","doi":"10.18173/2354-1059.2021-0068","DOIUrl":"https://doi.org/10.18173/2354-1059.2021-0068","url":null,"abstract":"The experiment screening of potential cassava varieties for good tolerance to artificial waterlogging was evaluated on 17 varieties of cassava in vitro at 3 months of age after planting in potting soil for 12 days through physiological parameters: number of leavesplant, number of yellow leaves, number of wilted plants and chlorophyll content. The results showed that cassava varieties had different tolerance to waterlogging: some varieties showed physiological symptoms only after 3 days of growth on waterlogging conditions such as the number of leavesplant decreasing to 83.93 %. 93.75 %, 93.81 %, 95.88 % and 96.48 % in cassava varieties C86, C23, Hanoi3, C84 and C31, respectively. After 6 days of waterlogging, the percentage of yellow leaves in some varieties of cassava had been increased, attained up to 40 % of the total leavesplant turned yellow such as C71 (44.68 %) or C13 (53.12 %) varieties while other cassava varieties wilted and die 100 % such as Hanoi3, C10, C13, C34, C42, C66, C71, C84 and C86; along with the symtoms of morphological and growth of cassava varieties on waterlogging conditions, the total of chlorophyll content also decreased. After 9 days of waterlogging, only C60 variety remained chlorophyll content with 77.38 % but in other cassava varieties, the chlorophyll content was not more than 50 %. We founded that the longer the duration of waterlogging, the more tolerant response of the studied cassava varieties was revealed. After 12 days of experiment, we initially determined that the C60 variety was the best flood tolerance among the 17 studied varieties and this was a good source of materials on selecting the variety of flood tolerant cassava.","PeriodicalId":17007,"journal":{"name":"Journal of Science Natural Science","volume":"2 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79024082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-10-01DOI: 10.18173/2354-1059.2021-0051
Dao Pham Thi Bich, Ha Nguyen Thi Hoai, Cong Vu Thanh, Hoan Phung Van, Binh Tran Quoc, L. Nguyen Ngoc
C-phycocyanin (C-PC) was a phycobiliprotein found exclusively in cyanobacteria as Spirulina and exhibited a variety of pharmacological properties, which were not yet studied for any purpose in commercial applications in the pharmaceutical and cosmetic industry in Vietnam. At present, there are no other sources of natural phycocyanin than Spirulina, it is the sole efficient source of natural phycocyanin production under photoautotrophic conditions. The present research was conducted to determine an efficient extraction method from C-PC from Spirulina platensis dry biomass. Antioxidant activity of purified C-PC was investigated. Extractions were carried out using distilled water and Na-phosphate buffer pH 7.0 as solvents and freezethaw and ultrasonication techniques were applied to optimize the extraction process. Extraction using the freezethaw method proved to be the most efficient method. Phycocyanin purification from crude extracts was carried out by a combination of methods such as ammonium sulfate (NH4)2SO4 65% precipitation, dialysis filter, and Sephadex G-100 gel filtration chromatography, to give 121.63 ± 0.03 mgg C-PC content and 3.52 ± 0.04 purity. Antioxidant activity in vitro was determined by nitric oxide (NO) scavenging activity, percentage inhibition of NO by the C-PC in concentration 10 μgmL to 100 μgmL which significantly scavenged 55.89% to 91.54% of NO radicals, respectively. They exhibited strong NO radical scavenging capacity, the C-PC and vitamin C were able to reduce the stable NO radical to 50% reduction with EC50 of 4.53 and 3.66 µgmL. Research results showed that S. platenis dry biomass provided by Dalitra was potential for obtaining C-phycocyanin (12.16% of dry biomass) and it was evaluated as an antioxidant in vitro able to scavenge nitric oxide. C-PC (12.16% of dry biomass) and was evaluated as an antioxidant in vitro able to scavenge nitric oxide.
c -藻蓝蛋白(C-PC)是一种仅存在于螺旋藻等蓝藻细菌中的藻胆蛋白，具有多种药理特性，在越南尚未对其在制药和化妆品工业中的商业应用进行研究。目前，除了螺旋藻之外，没有其他天然藻蓝蛋白的来源，它是光自养条件下唯一有效的天然藻蓝蛋白生产来源。本研究旨在确定从螺旋藻干生物量中提取C-PC的有效方法。研究了纯化的C-PC的抗氧化活性。以蒸馏水和pH 7.0的Na-phosphate缓冲液为溶剂，采用冷冻/解冻和超声波技术对提取工艺进行优化。冻融法是最有效的提取方法。采用硫酸铵(NH4)2SO4 65%沉淀、透析过滤、Sephadex g -100凝胶过滤层析等方法对粗提物中藻蓝蛋白进行纯化，得到C-PC含量121.63±0.03 mgg，纯度3.52±0.04。体外抗氧化活性测定以10 ~ 100 μgmL浓度的C-PC对一氧化氮(NO)的清除活性和对NO的抑制率为指标，分别显著清除55.89% ~ 91.54%的NO自由基。C- pc和维生素C具有较强的NO自由基清除能力，可将稳定的NO自由基减少50%，EC50分别为4.53和3.66µgmL。研究结果表明，由Dalitra提供的S. platenis干生物量有潜力获得c -藻蓝蛋白(占干生物量的12.16%)，并被评价为具有清除一氧化氮的体外抗氧化剂。C-PC(占干生物量的12.16%)，并被评价为能够清除一氧化氮的体外抗氧化剂。
{"title":"IN VITRO ANTIOXIDANT ACTIVITY OF C-PHYCOCYANIN PURIFIED FROM Spirulina platensis DRY BIOMASS","authors":"Dao Pham Thi Bich, Ha Nguyen Thi Hoai, Cong Vu Thanh, Hoan Phung Van, Binh Tran Quoc, L. Nguyen Ngoc","doi":"10.18173/2354-1059.2021-0051","DOIUrl":"https://doi.org/10.18173/2354-1059.2021-0051","url":null,"abstract":"C-phycocyanin (C-PC) was a phycobiliprotein found exclusively in cyanobacteria as Spirulina and exhibited a variety of pharmacological properties, which were not yet studied for any purpose in commercial applications in the pharmaceutical and cosmetic industry in Vietnam. At present, there are no other sources of natural phycocyanin than Spirulina, it is the sole efficient source of natural phycocyanin production under photoautotrophic conditions. The present research was conducted to determine an efficient extraction method from C-PC from Spirulina platensis dry biomass. Antioxidant activity of purified C-PC was investigated. Extractions were carried out using distilled water and Na-phosphate buffer pH 7.0 as solvents and freezethaw and ultrasonication techniques were applied to optimize the extraction process. Extraction using the freezethaw method proved to be the most efficient method. Phycocyanin purification from crude extracts was carried out by a combination of methods such as ammonium sulfate (NH4)2SO4 65% precipitation, dialysis filter, and Sephadex G-100 gel filtration chromatography, to give 121.63 ± 0.03 mgg C-PC content and 3.52 ± 0.04 purity. Antioxidant activity in vitro was determined by nitric oxide (NO) scavenging activity, percentage inhibition of NO by the C-PC in concentration 10 μgmL to 100 μgmL which significantly scavenged 55.89% to 91.54% of NO radicals, respectively. They exhibited strong NO radical scavenging capacity, the C-PC and vitamin C were able to reduce the stable NO radical to 50% reduction with EC50 of 4.53 and 3.66 µgmL. Research results showed that S. platenis dry biomass provided by Dalitra was potential for obtaining C-phycocyanin (12.16% of dry biomass) and it was evaluated as an antioxidant in vitro able to scavenge nitric oxide. C-PC (12.16% of dry biomass) and was evaluated as an antioxidant in vitro able to scavenge nitric oxide.","PeriodicalId":17007,"journal":{"name":"Journal of Science Natural Science","volume":"512 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78144379","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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