Journal of Stored Products Research最新文献_第6页

Transcriptome reveals patterns of response by Lasioderma serricorne (Coleoptera: Ptinidae) larvae to s-methoprene exposure

IF 2.7 2区农林科学 Q1 ENTOMOLOGY

Journal of Stored Products Research

Pub Date : 2024-12-24 DOI: 10.1016/j.jspr.2024.102521

Chao Huang , Jianhua Lü , Chunqi Bai , Yafei Guo , Chao Guo , Jizhen Song , Jiaqin Xi

Lasioderma serricorne is an important pest in the storage of tobacco and grains. s-methoprene is among the most widely used juvenile hormone analogues (JHA). However, the molecular mechanism of s-methoprene on Lasioderma serricorne was not clear. Thus, the survival number, pupation rate, mortality rate, and transcriptome of L. serricorne larvae (Larvae of L. serricorne exposed to s-methoprene at 0.0, 0.5, 5.0, and 50.0 × 10⁻⁵ mg/cm² for 2 weeks were referred to as LS0, LS0.5, LS5, and LS50, respectively) exposed to different doses of s-methoprene were studied. After 2 weeks of exposure to s-methoprene, there were significant differences in the number of larvae and pupation rate between the treatment and control groups. By analyzing the gene expression patterns and whole transcriptome of L. serricorne larvae, a total of 468, 4003 and 5132 differentially expressed genes (DEGs) were identified (LS0.5 vs LS0, LS5 vs LS0, and LS50 vs LS0, respectively). A total of 120 commonly up-regulated genes and 3659 commonly down-regulated genes were identified in LS5 vs LS0, and LS50 vs LS0. Weighted gene correlation expression network analysis (WGCNA) identified five modules comprising all transcripts, with the blue module closely related to the LS50. Moreover, the genes of the blue module were highly correlated with “biological processes (1162 genes)”, “cellular components (1507 genes)”, and “molecular functions (1446 genes)”. Three transcription factor genes TRINITY_DN3652_c0_g1_i12, TRINITY_DN6508_c0_g2_i1 and TRINITY_DN184_c0_g2_i2 were identified as hub genes in the blue module. The expression changes of these genes indicated that the expression levels of non-biological related genes, such as enzymes, protein metabolism, and signal transduction may change under s-methoprene stress. The present results revealed the molecular mechanism of the adaptability of L. serricorne to s-methoprene stress, providing a reference for further exploring the molecular mechanism of s-methoprene on L. serricorne.

{"title":"Transcriptome reveals patterns of response by Lasioderma serricorne (Coleoptera: Ptinidae) larvae to s-methoprene exposure","authors":"Chao Huang , Jianhua Lü , Chunqi Bai , Yafei Guo , Chao Guo , Jizhen Song , Jiaqin Xi","doi":"10.1016/j.jspr.2024.102521","DOIUrl":"10.1016/j.jspr.2024.102521","url":null,"abstract":"<div><div><em>Lasioderma serricorn</em>e is an important pest in the storage of tobacco and grains. <em>s</em>-methoprene is among the most widely used juvenile hormone analogues (JHA). However, the molecular mechanism of <em>s</em>-methoprene on <em>Lasioderma serricorn</em>e was not clear. Thus, the survival number, pupation rate, mortality rate, and transcriptome of <em>L. serricorne</em> larvae (Larvae of L. <em>serricorne</em> exposed to <em>s</em>-methoprene at 0.0, 0.5, 5.0, and 50.0 × 10<sup>−5</sup> mg/cm<sup>2</sup> for 2 weeks were referred to as LS0, LS0.5, LS5, and LS50, respectively) exposed to different doses of <em>s</em>-methoprene were studied. After 2 weeks of exposure to <em>s</em>-methoprene, there were significant differences in the number of larvae and pupation rate between the treatment and control groups. By analyzing the gene expression patterns and whole transcriptome of <em>L. serricorne</em> larvae, a total of 468, 4003 and 5132 differentially expressed genes (DEGs) were identified (LS0.5 <em>vs</em> LS0, LS5 <em>vs</em> LS0, and LS50 <em>vs</em> LS0, respectively). A total of 120 commonly up-regulated genes and 3659 commonly down-regulated genes were identified in LS5 <em>vs</em> LS0, and LS50 <em>vs</em> LS0. Weighted gene correlation expression network analysis (WGCNA) identified five modules comprising all transcripts, with the blue module closely related to the LS50. Moreover, the genes of the blue module were highly correlated with “biological processes (1162 genes)”, “cellular components (1507 genes)”, and “molecular functions (1446 genes)”. Three transcription factor genes TRINITY_DN3652_c0_g1_i12, TRINITY_DN6508_c0_g2_i1 and TRINITY_DN184_c0_g2_i2 were identified as hub genes in the blue module. The expression changes of these genes indicated that the expression levels of non-biological related genes, such as enzymes, protein metabolism, and signal transduction may change under <em>s</em>-methoprene stress. The present results revealed the molecular mechanism of the adaptability of <em>L. serricorne</em> to <em>s</em>-methoprene stress, providing a reference for further exploring the molecular mechanism of <em>s</em>-methoprene on <em>L. serricorne</em>.</div></div>","PeriodicalId":17019,"journal":{"name":"Journal of Stored Products Research","volume":"111 ","pages":"Article 102521"},"PeriodicalIF":2.7,"publicationDate":"2024-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143100503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Developing commercial-scale fresh sweetpotato root storage in tropical areas of sub-Saharan Africa

IF 2.7 2区农林科学 Q1 ENTOMOLOGY

Journal of Stored Products Research

Pub Date : 2024-12-23 DOI: 10.1016/j.jspr.2024.102522

Tanya Stathers , Benard Otieno , Bethwel Kipkoech , Debbie Rees , Marcelo Precoppe , Penina Muoki , Jan Low

As food systems across sub-Saharan Africa transform and urbanise, demand for all-year-round supplies for the raw materials is increasing. This demand can be met through combinations of staggering crop production, purchasing from diverse geographical areas, and storing produce. Focusing on the increasing use of orange-fleshed sweetpotato root puree in vitamin-A-rich food products, we ran a series of storage trials in Kenya to investigate whether commercial-scale fresh orange-fleshed sweetpotato storage could provide a stable supply of roots for puree production. The trials studied storage of roots of two sweetpotato varieties (Kabode and Vita), using washed versus unwashed roots in wooden crates, and mains grid power versus off-grid solar-powered storerooms for four-months. Following curing at 30 °C, roots were stored at 20–23 °C, and quality assessed. After four months storage, 54–59 % (Kabode) and 63–83 % (Vita) of initial root weight remained suitable for processing into puree. However, weevil and sprouting problems occurred. Vita outperformed Kabode for most criteria. Neither root washing nor different storerooms had a consistent effect on root quality. Subsequent trials in a solar-powered store investigated if lower temperatures of ≤15 °C and 90 % rh, with pre-harvest dehaulming could reduce weevil development, sprouting and rotting in stored roots. While control of sprouting and weevil damage was achieved and dehaulming improved stored root quality, while washing reduced it, high incidence of rotting and root weight loss occurred. Increasing store ventilation did not reduce rotting. We conclude that further trials with well controlled storage environments are required to understand how raw root quality, dehaulming, harvesting and handling practices, curing conditions, air exchange, and packaging materials affect quality during fresh root storage.

{"title":"Developing commercial-scale fresh sweetpotato root storage in tropical areas of sub-Saharan Africa","authors":"Tanya Stathers , Benard Otieno , Bethwel Kipkoech , Debbie Rees , Marcelo Precoppe , Penina Muoki , Jan Low","doi":"10.1016/j.jspr.2024.102522","DOIUrl":"10.1016/j.jspr.2024.102522","url":null,"abstract":"<div><div>As food systems across sub-Saharan Africa transform and urbanise, demand for all-year-round supplies for the raw materials is increasing. This demand can be met through combinations of staggering crop production, purchasing from diverse geographical areas, and storing produce. Focusing on the increasing use of orange-fleshed sweetpotato root puree in vitamin-A-rich food products, we ran a series of storage trials in Kenya to investigate whether commercial-scale fresh orange-fleshed sweetpotato storage could provide a stable supply of roots for puree production. The trials studied storage of roots of two sweetpotato varieties (Kabode and Vita), using washed versus unwashed roots in wooden crates, and mains grid power versus off-grid solar-powered storerooms for four-months. Following curing at 30 °C, roots were stored at 20–23 °C, and quality assessed. After four months storage, 54–59 % (Kabode) and 63–83 % (Vita) of initial root weight remained suitable for processing into puree. However, weevil and sprouting problems occurred. Vita outperformed Kabode for most criteria. Neither root washing nor different storerooms had a consistent effect on root quality. Subsequent trials in a solar-powered store investigated if lower temperatures of ≤15 °C and 90 % rh, with pre-harvest dehaulming could reduce weevil development, sprouting and rotting in stored roots. While control of sprouting and weevil damage was achieved and dehaulming improved stored root quality, while washing reduced it, high incidence of rotting and root weight loss occurred. Increasing store ventilation did not reduce rotting. We conclude that further trials with well controlled storage environments are required to understand how raw root quality, dehaulming, harvesting and handling practices, curing conditions, air exchange, and packaging materials affect quality during fresh root storage.</div></div>","PeriodicalId":17019,"journal":{"name":"Journal of Stored Products Research","volume":"111 ","pages":"Article 102522"},"PeriodicalIF":2.7,"publicationDate":"2024-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143100502","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mechanisms behind the post harvest sprouting of onions (allium cepa) and future implications of prevention strategies

IF 2.7 2区农林科学 Q1 ENTOMOLOGY

Journal of Stored Products Research

Pub Date : 2024-12-23 DOI: 10.1016/j.jspr.2024.102518

Mahesh Kumar Samota , Shilpa S. Selvan , Poonam Choudhary , Amit Nath , Arvind Ahlawat , Varinda

Globally, onions are an important vegetables used primarily as a flavoring agents. Onion Bulbs has three major stage in physiology viz rest, dormancy and sprout. During post harvest storage sprouting is the major problem that causes loss of onions. The sprouting in bulbs controlled by different factors such as concentration of plant hormones, biochemical and molecular changes. Therefore, control of sprouting in onion bulbs is commercially crucial for shelf life management. At present, technologies like maleic hydrazide, 1-methyl cyclopropane, γ-radiation, and ozone used to prevent onion sprouting but these technologies have some negative impact on health so need to explore alternative prevention strategies. Recently, the role of various plant hormones in sprout initiation, elongation, and development in onions during storage was emerged. In this review, emphasized on onion physiology, hormonal genes associated with dormancy, and factor affecting the sprouting. Also, summarized the mechanism, physico-chemical changes and prevention strategies in onion bulbs during sprouting.

{"title":"Mechanisms behind the post harvest sprouting of onions (allium cepa) and future implications of prevention strategies","authors":"Mahesh Kumar Samota , Shilpa S. Selvan , Poonam Choudhary , Amit Nath , Arvind Ahlawat , Varinda","doi":"10.1016/j.jspr.2024.102518","DOIUrl":"10.1016/j.jspr.2024.102518","url":null,"abstract":"<div><div>Globally, onions are an important vegetables used primarily as a flavoring agents. Onion Bulbs has three major stage in physiology viz rest, dormancy and sprout. During post harvest storage sprouting is the major problem that causes loss of onions. The sprouting in bulbs controlled by different factors such as concentration of plant hormones, biochemical and molecular changes. Therefore, control of sprouting in onion bulbs is commercially crucial for shelf life management. At present, technologies like maleic hydrazide, 1-methyl cyclopropane, γ-radiation, and ozone used to prevent onion sprouting but these technologies have some negative impact on health so need to explore alternative prevention strategies. Recently, the role of various plant hormones in sprout initiation, elongation, and development in onions during storage was emerged. In this review, emphasized on onion physiology, hormonal genes associated with dormancy, and factor affecting the sprouting. Also, summarized the mechanism, physico-chemical changes and prevention strategies in onion bulbs during sprouting.</div></div>","PeriodicalId":17019,"journal":{"name":"Journal of Stored Products Research","volume":"111 ","pages":"Article 102518"},"PeriodicalIF":2.7,"publicationDate":"2024-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143104916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Morphological and molecular characterization of major internal feeders in storage insect pests using SWISS- model

IF 2.7 2区农林科学 Q1 ENTOMOLOGY

Journal of Stored Products Research

Pub Date : 2024-12-22 DOI: 10.1016/j.jspr.2024.102523

B. Keerthana , G. Preetha , V.R. Saminathan , M. Murugan , T. Eevera , D. Ramesh

The present investigation focuses on morphological, morphometrics and molecular characterization of four significant insect pests of stored grains, Callosobruchus maculatus (F.), Callosobruchus chinensis (L.), Caryedon serratus (Olivier) and Sitophilus oryzae (L.). Detailed morphological descriptions of egg, larval, pupal and adult stages, including color patterns, anatomical features and genital structures were provided for each species. Morphometric measurements of various life stages of each pest was recorded. Molecular characterization of insects involved extracting genomic DNA and utilizing mitochondrial cytochrome oxidase I (mt COI) as a molecular marker to identify and differentiate the species. Sequencing and phylogenetic analysis confirmed species identities, revealing high genetic similarities with previously documented populations. The sequences were submitted to GenBank and an accession number was generated for Callosobruchus maculatus, Callosobruchus chinensis, Caryedon serratus, and Sitophilus oryzae. Additionally, protein modeling and structure validation were conducted using SWISS-MODEL with Ramachandran plots confirming the high quality of the predicted protein structures. This study aids in accurately identifying and understanding these pests morphology, morphometrics and molecular characterization and contributes to more effective pest management strategies.

{"title":"Morphological and molecular characterization of major internal feeders in storage insect pests using SWISS- model","authors":"B. Keerthana , G. Preetha , V.R. Saminathan , M. Murugan , T. Eevera , D. Ramesh","doi":"10.1016/j.jspr.2024.102523","DOIUrl":"10.1016/j.jspr.2024.102523","url":null,"abstract":"<div><div>The present investigation focuses on morphological, morphometrics and molecular characterization of four significant insect pests of stored grains, <em>Callosobruchus maculatus</em> (F.), <em>Callosobruchus chinensis</em> (L.), <em>Caryedon serratus</em> (Olivier) and <em>Sitophilus oryzae</em> (L.). Detailed morphological descriptions of egg, larval, pupal and adult stages, including color patterns, anatomical features and genital structures were provided for each species. Morphometric measurements of various life stages of each pest was recorded. Molecular characterization of insects involved extracting genomic DNA and utilizing mitochondrial cytochrome oxidase I (mt COI) as a molecular marker to identify and differentiate the species. Sequencing and phylogenetic analysis confirmed species identities, revealing high genetic similarities with previously documented populations. The sequences were submitted to GenBank and an accession number was generated for <em>Callosobruchus maculatus, Callosobruchus chinensis, Caryedon serratus,</em> and <em>Sitophilus oryzae</em>. Additionally, protein modeling and structure validation were conducted using SWISS-MODEL with Ramachandran plots confirming the high quality of the predicted protein structures. This study aids in accurately identifying and understanding these pests morphology, morphometrics and molecular characterization and contributes to more effective pest management strategies.</div></div>","PeriodicalId":17019,"journal":{"name":"Journal of Stored Products Research","volume":"111 ","pages":"Article 102523"},"PeriodicalIF":2.7,"publicationDate":"2024-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143104471","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Changes in intergranular air properties and fatty acid profile of wheat bulk in a large warehouse with air-conditioned oversummering

IF 2.7 2区农林科学 Q1 ENTOMOLOGY

Journal of Stored Products Research

Pub Date : 2024-12-20 DOI: 10.1016/j.jspr.2024.102520

Mengya Wang , Minghui Zhao , Xuhui Zhuang , Jianzhang Wu , Xingjun Li , Linlin Song , Yushan Jiang

Effect of air-condition on wheat's oversummering in a large flat warehouse urgently need to study. In this study, the air-condition was set at 25 °C in the second and third summer of wheat storage in No.19 warehouse of Pingyuan Grain Storage Depot, Shandong province, China. The intergranular air properties of grain bulk were calculated by our developed software. Compared with the control warehouse (No.17), the surface, second, third, and fourth layers of wheat bulk in No.19 warehouse during the running of air-conditioners decreased by 3.4 °C, 2 °C, 1.5 °C and 1.35 °C, respectively, the humidity ratio, relative humidity and wet-bulb temperature in the surface layer and whole bulk respectively decreased by 3.85 and 1.5 g/kg, 3.55% and 3.85%, 3.65 °C and 2.2 °C. Air-conditioning significantly decreased the bulk surface temperature. Free fatty acid (FFA) values in each layer of grain bulk increased continuously since warehousing, and the average FFA value in No.17 warehouse managed by conventional technology was below 40 mg KOH/100g. Twenty-seven species of fatty acids were identified in wheat. Contents of the total and unsaturated fatty acids increased respectively from 11703 to 9348 μg/g at 124-day storage to 14758 and 11834 μg/g at 841-day storage for wheat bulk in No.17 warehouse, but increased respectively from 11674 to 9321 μg/g to 16235 and 13027 μg/g for grain bulk in No.19 warehouse. Infrared spectroscopy confirmed that during 841-day storage, the absorption values of C-H stretching vibration (2919.7 cm⁻¹) of saturated carbon and C-H stretching vibration (3304.9 cm⁻¹) of unsaturated carbon in No.19 warehouse were significantly higher than those of No.17 warehouse. Scanning electron microscopy shows that air-conditioned warehouse effectively maintain the internal distinct microstructure of wheat grains. These results suggest that air-conditioning can reduce the bulk surface temperature and FAA value of stored wheat and keep the content of unsaturated fatty acids, and FFA value of properly stored wheat can be assumed as ≤50 mg KOH/100g.

{"title":"Changes in intergranular air properties and fatty acid profile of wheat bulk in a large warehouse with air-conditioned oversummering","authors":"Mengya Wang , Minghui Zhao , Xuhui Zhuang , Jianzhang Wu , Xingjun Li , Linlin Song , Yushan Jiang","doi":"10.1016/j.jspr.2024.102520","DOIUrl":"10.1016/j.jspr.2024.102520","url":null,"abstract":"<div><div>Effect of air-condition on wheat's oversummering in a large flat warehouse urgently need to study. In this study, the air-condition was set at 25 °C in the second and third summer of wheat storage in No.19 warehouse of Pingyuan Grain Storage Depot, Shandong province, China. The intergranular air properties of grain bulk were calculated by our developed software. Compared with the control warehouse (No.17), the surface, second, third, and fourth layers of wheat bulk in No.19 warehouse during the running of air-conditioners decreased by 3.4 °C, 2 °C, 1.5 °C and 1.35 °C, respectively, the humidity ratio, relative humidity and wet-bulb temperature in the surface layer and whole bulk respectively decreased by 3.85 and 1.5 g/kg, 3.55% and 3.85%, 3.65 °C and 2.2 °C. Air-conditioning significantly decreased the bulk surface temperature. Free fatty acid (FFA) values in each layer of grain bulk increased continuously since warehousing, and the average FFA value in No.17 warehouse managed by conventional technology was below 40 mg KOH/100g. Twenty-seven species of fatty acids were identified in wheat. Contents of the total and unsaturated fatty acids increased respectively from 11703 to 9348 μg/g at 124-day storage to 14758 and 11834 μg/g at 841-day storage for wheat bulk in No.17 warehouse, but increased respectively from 11674 to 9321 μg/g to 16235 and 13027 μg/g for grain bulk in No.19 warehouse. Infrared spectroscopy confirmed that during 841-day storage, the absorption values of C-H stretching vibration (2919.7 cm<sup>−1</sup>) of saturated carbon and C-H stretching vibration (3304.9 cm<sup>−1</sup>) of unsaturated carbon in No.19 warehouse were significantly higher than those of No.17 warehouse. Scanning electron microscopy shows that air-conditioned warehouse effectively maintain the internal distinct microstructure of wheat grains. These results suggest that air-conditioning can reduce the bulk surface temperature and FAA value of stored wheat and keep the content of unsaturated fatty acids, and FFA value of properly stored wheat can be assumed as ≤50 mg KOH/100g.</div></div>","PeriodicalId":17019,"journal":{"name":"Journal of Stored Products Research","volume":"111 ","pages":"Article 102520"},"PeriodicalIF":2.7,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143104470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Application of reuterin-containing edible coating to inhibit the growth of aflatoxigenic Aspergillus flavus on nutmeg

IF 2.7 2区农林科学 Q1 ENTOMOLOGY

Journal of Stored Products Research

Pub Date : 2024-12-18 DOI: 10.1016/j.jspr.2024.102519

Widiati Purnawita , Winiati Pudji Rahayu , Hanifah Nuryani Lioe , Siti Nurjanah

Contamination of Aspergillus flavus (A. flavus) and aflatoxin in nutmeg remains problematic due to the poor postharvest handling practices and the climate, which is favorable for fungal growth in Indonesia. Using the natural antifungal agents can be an alternative measure to control the aflatoxin-producing fungal growth. This current study aimed to evaluate the efficacy of reuterin in inhibiting aflatoxigenic A. flavus growth on the nutmeg during storage. Reuterin at concentrations of 4, 6, 8, and 10 × MIC (1 MIC = 6 mM) was incorporated into a carboxymethyl cellulose (CMC)-based edible coating to coat the surface of nutmeg spiked with A. flavus conidia (2.5 × 10² CFU/g). Results showed that reuterin effectively extended the lag phase of A. flavus growth on the coated nutmeg and disrupted the hyphal development and conidiation. At a minimum concentration of 48 mM (equal to 3.63 mg/mL coating solution or 630 mg/kg nutmeg), reuterin inhibited completely the growth of A. flavus on the nutmeg stored at an RH of 97% and a temperature of 30 °C for 30 days. This study confirmed that the application of reuterin incorporated into a CMC-based edible coating was an effective measure for inhibiting aflatoxigenic A. flavus growth on the nutmeg during storage.

{"title":"Application of reuterin-containing edible coating to inhibit the growth of aflatoxigenic Aspergillus flavus on nutmeg","authors":"Widiati Purnawita , Winiati Pudji Rahayu , Hanifah Nuryani Lioe , Siti Nurjanah","doi":"10.1016/j.jspr.2024.102519","DOIUrl":"10.1016/j.jspr.2024.102519","url":null,"abstract":"<div><div>Contamination of <em>Aspergillus flavus</em> (<em>A. flavus</em>) and aflatoxin in nutmeg remains problematic due to the poor postharvest handling practices and the climate, which is favorable for fungal growth in Indonesia. Using the natural antifungal agents can be an alternative measure to control the aflatoxin-producing fungal growth. This current study aimed to evaluate the efficacy of reuterin in inhibiting aflatoxigenic <em>A. flavus</em> growth on the nutmeg during storage. Reuterin at concentrations of 4, 6, 8, and 10 × MIC (1 MIC = 6 mM) was incorporated into a carboxymethyl cellulose (CMC)-based edible coating to coat the surface of nutmeg spiked with <em>A. flavus</em> conidia (2.5 × 10<sup>2</sup> CFU/g). Results showed that reuterin effectively extended the lag phase of <em>A. flavus</em> growth on the coated nutmeg and disrupted the hyphal development and conidiation. At a minimum concentration of 48 mM (equal to 3.63 mg/mL coating solution or 630 mg/kg nutmeg), reuterin inhibited completely the growth of <em>A. flavus</em> on the nutmeg stored at an RH of 97% and a temperature of 30 °C for 30 days. This study confirmed that the application of reuterin incorporated into a CMC-based edible coating was an effective measure for inhibiting aflatoxigenic <em>A. flavus</em> growth on the nutmeg during storage.</div></div>","PeriodicalId":17019,"journal":{"name":"Journal of Stored Products Research","volume":"111 ","pages":"Article 102519"},"PeriodicalIF":2.7,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143104469","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sublethal impact of pirimiphos-methyl and etofenprox on mandible and hindwing morphology in Alphitobius diaperinus

IF 2.7 2区农林科学 Q1 ENTOMOLOGY

Journal of Stored Products Research

Pub Date : 2024-12-16 DOI: 10.1016/j.jspr.2024.102482

Demeter Lorentha S. Gidari , Vladimir Žikić , Maria C. Boukouvala , Nickolas G. Kavallieratos , Maja Lazarević

Insecticidal applications can cause morphological deformities to the exposed insects or their offspring. Pirimiphos-methyl and etofenprox have been previously evaluated against Alphitobius diaperinus, but there is no knowledge about their effect on the morphology of the F1 generation. This study examines the effect of both insecticides on mandible and hindwing morphology of A. diaperinus adult progeny. Regarding the shape of the mandibles in both female and male individuals, the two insecticides induced significant deformations compared to the control group. Etofenprox caused more vigorous morphological changes vs. pirimiphos-methyl. In terms of hindwings, Principal Component Analysis (PCA) did not reveal distinction between females or males exposed to either etofenprox or pirimiphos-methyl. Both insecticides affected the mouthparts of the F1 generation adults of A. diaperinus, which could lead to reduced feeding ability and thus to insufficient population growth.

{"title":"Sublethal impact of pirimiphos-methyl and etofenprox on mandible and hindwing morphology in Alphitobius diaperinus","authors":"Demeter Lorentha S. Gidari , Vladimir Žikić , Maria C. Boukouvala , Nickolas G. Kavallieratos , Maja Lazarević","doi":"10.1016/j.jspr.2024.102482","DOIUrl":"10.1016/j.jspr.2024.102482","url":null,"abstract":"<div><div>Insecticidal applications can cause morphological deformities to the exposed insects or their offspring. Pirimiphos-methyl and etofenprox have been previously evaluated against <em>Alphitobius diaperinus,</em> but there is no knowledge about their effect on the morphology of the F1 generation. This study examines the effect of both insecticides on mandible and hindwing morphology of <em>A. diaperinus</em> adult progeny. Regarding the shape of the mandibles in both female and male individuals, the two insecticides induced significant deformations compared to the control group. Etofenprox caused more vigorous morphological changes vs. pirimiphos-methyl. In terms of hindwings, Principal Component Analysis (PCA) did not reveal distinction between females or males exposed to either etofenprox or pirimiphos-methyl. Both insecticides affected the mouthparts of the F1 generation adults of <em>A. diaperinus</em>, which could lead to reduced feeding ability and thus to insufficient population growth.</div></div>","PeriodicalId":17019,"journal":{"name":"Journal of Stored Products Research","volume":"111 ","pages":"Article 102482"},"PeriodicalIF":2.7,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143180505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bioactive composition, microstructure, and physicochemical properties of Arbutus unedo berries dried using different techniques

IF 2.7 2区农林科学 Q1 ENTOMOLOGY

Journal of Stored Products Research

Pub Date : 2024-12-14 DOI: 10.1016/j.jspr.2024.102501

Ibtissem Refas , Malek Amiali , Oluwafemi Ayodele George , Kieu Hiep Le , Narjes Malekjani , Abdolreza Kharaghani

This research examined the impact of infrared (IR) and convective drying (CV) at varying temperatures (40, 60, and 80 °C) and freeze-drying (FD) on the microstructure, drying kinetics, and quality attributes of strawberry tree fruit (Arbutus unedo L.), including phenolic content (TPC), flavonoids (TFC), anthocyanins (TA), antioxidant activity, color, rehydration ratio, shrinkage, and bulk density. Results showed that elevated temperatures accelerated the drying process, with IR at 80 °C reducing the duration by 69% compared to CV. IR drying was particularly effective in preserving the fruit's red color, achieving the lowest ΔE values (8 ± 4.59) and showing minimal color change at 80 °C. While drying generally increased TFC and TPC, the impact on antioxidant activity was negligible, except in IR drying at 60 °C. However, TA was largely reduced, mainly due to prolonged processing times. SEM analysis highlighted that FD samples maintained a more open structure, while IR samples showed desirable microstructure preservation, leading to improved moisture retention. The Midilli model best described the drying process of the strawberry tree berries, with diffusivity ranging from 0.96 to 7.07 ( × 10⁻¹⁰ m²/s) for CV and 0.42 to 2.57 ( × 10⁻⁹ m²/s) for IR. Activation energies were calculated as 45.72 kJ/mol for CV and 41.91 kJ/mol for IR. Overall, the study suggests that IR at 80 °C is the most efficient method for drying Arbutus unedo, based on its effectiveness in drying time reduction, preservation of color, anthocyanins, microstructural integrity, and minimizing shrinkage, while maximizing the rehydration ratio compared to CV.

{"title":"Bioactive composition, microstructure, and physicochemical properties of Arbutus unedo berries dried using different techniques","authors":"Ibtissem Refas , Malek Amiali , Oluwafemi Ayodele George , Kieu Hiep Le , Narjes Malekjani , Abdolreza Kharaghani","doi":"10.1016/j.jspr.2024.102501","DOIUrl":"10.1016/j.jspr.2024.102501","url":null,"abstract":"<div><div>This research examined the impact of infrared (IR) and convective drying (CV) at varying temperatures (40, 60, and 80 °C) and freeze-drying (FD) on the microstructure, drying kinetics, and quality attributes of strawberry tree fruit (<em>Arbutus unedo</em> L.), including phenolic content (TPC), flavonoids (TFC), anthocyanins (TA), antioxidant activity, color, rehydration ratio, shrinkage, and bulk density. Results showed that elevated temperatures accelerated the drying process, with IR at 80 °C reducing the duration by 69% compared to CV. IR drying was particularly effective in preserving the fruit's red color, achieving the lowest ΔE values (8 ± 4.59) and showing minimal color change at 80 °C. While drying generally increased TFC and TPC, the impact on antioxidant activity was negligible, except in IR drying at 60 °C. However, TA was largely reduced, mainly due to prolonged processing times. SEM analysis highlighted that FD samples maintained a more open structure, while IR samples showed desirable microstructure preservation, leading to improved moisture retention. The Midilli model best described the drying process of the strawberry tree berries, with diffusivity ranging from 0.96 to 7.07 ( × 10<sup>−10</sup> m<sup>2</sup>/s) for CV and 0.42 to 2.57 ( × 10<sup>−9</sup> m<sup>2</sup>/s) for IR. Activation energies were calculated as 45.72 kJ/mol for CV and 41.91 kJ/mol for IR. Overall, the study suggests that IR at 80 °C is the most efficient method for drying <em>Arbutus unedo</em>, based on its effectiveness in drying time reduction, preservation of color, anthocyanins, microstructural integrity, and minimizing shrinkage, while maximizing the rehydration ratio compared to CV.</div></div>","PeriodicalId":17019,"journal":{"name":"Journal of Stored Products Research","volume":"111 ","pages":"Article 102501"},"PeriodicalIF":2.7,"publicationDate":"2024-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143104467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chemical compositions and insecticidal activities of Tetradium ruticarpum essential oils against Lasioderma serricorne

IF 2.7 2区农林科学 Q1 ENTOMOLOGY

Journal of Stored Products Research

Pub Date : 2024-12-14 DOI: 10.1016/j.jspr.2024.102517

Yu-Chen Zhang , Ao Liu , Jia-Zhu Wang , Yuan-Tong Qi , Shu-Shan Du

Stored products are susceptible to insect infestation. Many plant essential oils (EOs) have been used to control various pests, and compared to conventional pesticides, these natural products generally pose lower risks to humans and the environment. This study aimed to evaluate the insecticidal and repellent properties of EOs extracted from Tetradium ruticarpum (formerly Euodia rutaecarpa) fruits at different collection times against Lasioderma serricorne. EOs were obtained through hydrodistillation and analyzed using gas chromatography-mass spectrometry (GC-MS). The oil yield was 1.18 v/w. A total of 41 compounds were identified, with monoterpenes dominating in most samples. Except for the TRF-7 sample, where the main compound was 1,3-dicyclohexylpropane (45.9%), the predominant compound in all other EOs was 3-carene, with contents exceeding 87%. According to Principal Component Analysis (PCA), the composition of TRF-7 EO differed significantly from the other EOs due to the different collection times. The bio-assays showed that EOs demonstrated strong contact toxicity and repellent effects against L. serricorne, but fumigant toxicity was relatively weak. Among the tested samples, TRF-2 and TRF-4 exhibited the highest contact toxicity, with LD₅₀ values of 2.76 mg/L air and 4.08 mg/L air, respectively. Repellency was effective at higher concentrations but diminished with lower doses and extended exposure. These results suggest that EOs from T. ruticarpum fruits hold potential as botanical insecticides for protecting stored products.

{"title":"Chemical compositions and insecticidal activities of Tetradium ruticarpum essential oils against Lasioderma serricorne","authors":"Yu-Chen Zhang , Ao Liu , Jia-Zhu Wang , Yuan-Tong Qi , Shu-Shan Du","doi":"10.1016/j.jspr.2024.102517","DOIUrl":"10.1016/j.jspr.2024.102517","url":null,"abstract":"<div><div>Stored products are susceptible to insect infestation. Many plant essential oils (EOs) have been used to control various pests, and compared to conventional pesticides, these natural products generally pose lower risks to humans and the environment. This study aimed to evaluate the insecticidal and repellent properties of EOs extracted from <em>Tetradium ruticarpum</em> (formerly <em>Euodia rutaecarpa</em>) fruits at different collection times against <em>Lasioderma serricorne</em>. EOs were obtained through hydrodistillation and analyzed using gas chromatography-mass spectrometry (GC-MS). The oil yield was 1.18 v/w. A total of 41 compounds were identified, with monoterpenes dominating in most samples. Except for the TRF-7 sample, where the main compound was 1,3-dicyclohexylpropane (45.9%), the predominant compound in all other EOs was 3-carene, with contents exceeding 87%. According to Principal Component Analysis (PCA), the composition of TRF-7 EO differed significantly from the other EOs due to the different collection times. The bio-assays showed that EOs demonstrated strong contact toxicity and repellent effects against <em>L. serricorne</em>, but fumigant toxicity was relatively weak. Among the tested samples, TRF-2 and TRF-4 exhibited the highest contact toxicity, with LD<sub>50</sub> values of 2.76 mg/L air and 4.08 mg/L air, respectively. Repellency was effective at higher concentrations but diminished with lower doses and extended exposure. These results suggest that EOs from <em>T. ruticarpum</em> fruits hold potential as botanical insecticides for protecting stored products.</div></div>","PeriodicalId":17019,"journal":{"name":"Journal of Stored Products Research","volume":"111 ","pages":"Article 102517"},"PeriodicalIF":2.7,"publicationDate":"2024-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143104468","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Carrageenan based edible coating modulates oxidative stress and delays cell wall polysaccharides disassembly in harvested grapefruit

IF 2.7 2区农林科学 Q1 ENTOMOLOGY

Journal of Stored Products Research

Pub Date : 2024-12-13 DOI: 10.1016/j.jspr.2024.102514

Sajid Ali , Sidra Riaz , Hafiz Umer Javed , Ahmad Sattar Khan , Maratab Ali , Safina Naz , Hasan Sardar , Muhammad Shahzad Saleem

The cell wall degradation results in firmness loss thus negatively affecting the overall eating quality. So, in this work, the influence of carrageenan [0, 0.5, 1.0 and 1.5% (w/v)] coating was studied on ‘Shamber’ grapefruit during storage at 20 ± 1 °C for 42 days (d). The 1.5% carrageenan concentration suppressed metabolic activities and reduced disease rate (about 41%), weight loss (around 46%), malondialdehyde, electrolytes leakage, superoxide anion and hydrogen peroxide. The higher antioxidant enzyme activity as well as total flavonoids, ascorbic acid and total phenolic contents were noted in 1.5% treatment. The 1.5% coating delayed the increase in water-soluble pectin (WSP) and suppressed the reduction of sodium carbonate soluble pectin (NCSP), protopectin, chelate soluble pectin (CSP), hemicellulose and cellulose content. The grapefruit coated with 1.5% carrageenan had lower activities of cellulase (CEL), polygalacturonase (PG), β-galactosidase (β-gal) and pectin methylesterase (PME) enzyme along with higher firmness. The 1.5% carrageenan preserved higher titratable acidity and reduced increase in total soluble solids and ripening index in coated grapefruit. In conclusion, 1.5% carrageenan coating could be considered appropriate for delaying the quality deterioration in grapefruit.

{"title":"Carrageenan based edible coating modulates oxidative stress and delays cell wall polysaccharides disassembly in harvested grapefruit","authors":"Sajid Ali , Sidra Riaz , Hafiz Umer Javed , Ahmad Sattar Khan , Maratab Ali , Safina Naz , Hasan Sardar , Muhammad Shahzad Saleem","doi":"10.1016/j.jspr.2024.102514","DOIUrl":"10.1016/j.jspr.2024.102514","url":null,"abstract":"<div><div>The cell wall degradation results in firmness loss thus negatively affecting the overall eating quality. So, in this work, the influence of carrageenan [0, 0.5, 1.0 and 1.5% (w/v)] coating was studied on ‘Shamber’ grapefruit during storage at 20 ± 1 °C for 42 days (d). The 1.5% carrageenan concentration suppressed metabolic activities and reduced disease rate (about 41%), weight loss (around 46%), malondialdehyde, electrolytes leakage, superoxide anion and hydrogen peroxide. The higher antioxidant enzyme activity as well as total flavonoids, ascorbic acid and total phenolic contents were noted in 1.5% treatment. The 1.5% coating delayed the increase in water-soluble pectin (WSP) and suppressed the reduction of sodium carbonate soluble pectin (NCSP), protopectin, chelate soluble pectin (CSP), hemicellulose and cellulose content. The grapefruit coated with 1.5% carrageenan had lower activities of cellulase (CEL), polygalacturonase (PG), β-galactosidase (β-gal) and pectin methylesterase (PME) enzyme along with higher firmness. The 1.5% carrageenan preserved higher titratable acidity and reduced increase in total soluble solids and ripening index in coated grapefruit. In conclusion, 1.5% carrageenan coating could be considered appropriate for delaying the quality deterioration in grapefruit.</div></div>","PeriodicalId":17019,"journal":{"name":"Journal of Stored Products Research","volume":"111 ","pages":"Article 102514"},"PeriodicalIF":2.7,"publicationDate":"2024-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143104466","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0