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The impact of controlled atmosphere storage on maintaining physiological quality and productivity of soybean seeds 气调贮藏对保持大豆种子生理品质和产量的影响
IF 2.7 2区 农林科学 Q1 ENTOMOLOGY Pub Date : 2025-11-13 DOI: 10.1016/j.jspr.2025.102868
Magno Roberto Pasquetti Berghetti , Vagner Ludwig , Lucas Malmann Wendt , Francis Júnior Soldateli , Flavio Roberto Thewes , Fabiane Potella Rosato , Vanderlei Both , Diego Nicolau Follmann , Liege Camargo da Costa , Luciane Akmeri Tabaldi , Auri Brackmann
This study sought to investigate the efficacy of hermetic silos coupled with controlled atmospheres to extend quality of seeds. This study evaluated the influence of low partial oxygen, high carbon dioxide pressures and four storage temperatures (10, 15, 20, and 25 °C), on the physiological soybean quality seeds and subsequent crop yield. For the 2020/2021 season, soybeans seeds of NA 5909 RR cultivar, were stored for the conditions: {1} ambient, {2} 1.0 kPa O2 + 0.04 kPa CO2, and {3} 1.0 kPa O2 + 15.0 kPa CO2 for 6 months at 10, 15, 20, and 25 °C. In the 2021/2022 season, the conditions tested were: {1} ambient, {2} 1.0 kPa O2 + 0.0 kPa CO2, {3} 3.0 kPa O2 + 0.0 kPa CO2, and {4} 5.0 kPa O2 + 0.0 kPa CO2 at three temperatures (10, 15, and 20 °C). Moisture content, electrical conductivity, standard germination tests, seedling length and dry mass, hydrogen peroxide content, superoxide dismutase and guaiacol peroxidase activities were evaluated. After storage, the seeds were evaluated for field emergence, plant height, leaf area index, and yield. In the first and second year of evaluation, seeds stored at 10 °C under 1.0 kPa O2 show superior physiological quality in the assessed variables. In general, these condition (1.0 kPa O2 + 10 °C) increased field productivity, ranging from 82.0 to 421.0 kg ha−1 compared to ambient conditions and 268.7–978.0 kg ha−1 compared to seeds stored under other temperature conditions. High carbon dioxide pressures did not provide any additive benefit to low partial oxygen pressures in preserving the seeds physiological quality after storage. After six months of storage, all CA conditions maintained superior physiological quality of seeds, guaranteeing greater productivity, especially at low temperatures of 10 and 15 °C.
本研究旨在探讨密闭筒仓与控制气氛相结合对提高种子质量的效果。本研究评估了低偏氧、高二氧化碳压力和4种储存温度(10、15、20和25°C)对大豆生理品质种子和随后作物产量的影响。以2020/2021季NA 5909 RR品种大豆种子为研究对象,在10、15、20和25℃条件下,分别在{1}常温、{2}1.0 kPa O2 + 0.04 kPa CO2和{3}1.0 kPa O2 + 15.0 kPa CO2下贮藏6个月。在2021/2022赛季,测试条件为:{1}环境、{2}1.0 kPa O2 + 0.0 kPa CO2、{3}3.0 kPa O2 + 0.0 kPa CO2和{4}5.0 kPa O2 + 0.0 kPa CO2,温度分别为10、15和20℃。对水分含量、电导率、标准发芽试验、幼苗长和干质量、过氧化氢含量、超氧化物歧化酶和愈创木酚过氧化物酶活性进行了评价。贮藏后对种子进行出苗率、株高、叶面积指数和产量评价。在评价的第一年和第二年,在1.0 kPa O2和10°C条件下储存的种子在评价变量中表现出较好的生理品质。总的来说,与环境条件相比,这些条件(1.0 kPa O2 + 10°C)提高了田间产量,与其他温度条件相比,产量在82.0 ~ 421.0 kg ha - 1之间,在268.7 ~ 978.0 kg ha - 1之间。在贮藏后保持种子生理品质方面,高二氧化碳压力对低氧分压没有任何附加效益。经过6个月的贮藏,所有CA条件下的种子都保持了优异的生理品质,保证了更高的产量,特别是在10和15°C的低温条件下。
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引用次数: 0
Comparison of jute gunny bags with and without polypropylene for gram storage 含与不含聚丙烯的黄麻麻袋的克储存比较
IF 2.7 2区 农林科学 Q1 ENTOMOLOGY Pub Date : 2025-11-13 DOI: 10.1016/j.jspr.2025.102881
Abhinav Dubey , Indra Mani , Chandni , Roaf Ahmad Parray , Suresh Mahadev Nebapure , Shalini Gaur Rudra
Grain stored in jute bags is highly susceptible to loss, prompting the reinforcement of jute with polypropylene (PP) to improve storage characteristics. Jute fiber was reinforced with polypropylene in nine ratios (0–50 %) and evaluated for yarn strength and quality. Reinforced yarns were then woven into 50 kg storage bags for performance comparison when storing pulses (Bengal gram and green gram). Yarn count decreased by 7.1 %, while strength increased by 24.4 % with 50 % polypropylene. Fabric density and moisture regain decreased by 34 % and 59 %, respectively, enhancing moisture resistance and durability. Water vapor transmission rate was reduced by 39 %. The optimal blend of 58.5 % jute and 41.5 % polypropylene was found to be the most effective. The reinforced bags were also reported effectively biodegradable and having good stacking characteristics like jute bags as well as being biodegradable.
After six months of storage, the dry weight loss in Bengal gram and green gram was 26 % and 67 %, respectively, in jute bags, compared to under 5 % in reinforced bags. Grain damage and insect infestation were also significantly lower, with 82 % and 87 % grain damage in jute bags, reduced to 29 % and 22 % in reinforced bags for both crops. Moisture and protein content in stored grains were also better preserved. Economic analysis indicated that reinforced bags saved ₹ $7.32 – $13.04 per bag, while traditional jute bags incurred losses of $25.62 – $28.53 per bag, proving reinforced jute bags to be an efficient, sustainable and cost-effective alternative for grain storage.
储存在黄麻袋中的粮食极易损失,因此需要用聚丙烯(PP)加固黄麻以改善储存特性。用聚丙烯按9种配比(0 ~ 50%)对黄麻纤维进行增强,并对成纱强度和质量进行了评价。然后将增强纱线编织成50公斤的储存袋,用于储存豆类(孟加拉克和绿克)时的性能比较。添加50%聚丙烯后,纱线支数降低7.1%,而强度提高24.4%。织物密度和回潮率分别降低了34%和59%,增强了防潮性和耐久性。水蒸气透过率降低39%。结果表明,以58.5%黄麻和41.5%聚丙烯的最佳配比为最佳配比。增强袋也被报道有效地生物降解,具有像黄麻袋一样好的堆叠特性,并且是可生物降解的。贮藏6个月后,黄麻袋中的孟加拉克和绿克干重分别下降26%和67%,而加固袋中的干重下降不到5%。两种作物的粮食损失率和虫害发生率也显著降低,黄麻袋的损失率分别为82%和87%,加固袋的损失率分别为29%和22%。贮藏谷物的水分和蛋白质含量也得到了较好的保存。经济分析表明,加固袋每袋节省7.32 - 13.04美元,而传统的黄麻袋每袋损失25.62 - 28.53美元,证明加固袋是一种高效、可持续和具有成本效益的粮食储存替代方案。
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引用次数: 0
Characterization of the midgut proteome of Galleria mellonella L. (Lepidoptera: Pyralidae) larvae during development 千圆蛾(鳞翅目:蚜科)幼虫发育过程中肠道蛋白质组特征
IF 2.7 2区 农林科学 Q1 ENTOMOLOGY Pub Date : 2025-11-12 DOI: 10.1016/j.jspr.2025.102889
Güler Toprak , Nusret Ayyildiz , Abdurrahman Ayvaz , Dilek Kaan
Galleria mellonella, the bigger wax moth, plagues beekeeping. It is also a good model organism in host-pathogen studies and can digest polymers, making it an attractive biotechnology contender. In 2018, the G. mellonella genome was sequenced, allowing for additional proteomics-based study on this species' complex and dynamic biochemical processes. In this study, we characterized the effects of larval development on the midgut proteome by grouping the larvae based on their body weight, which was determined to correspond to the I, II, III, and IV instar stages. Proteins were isolated from dissected midguts using the TCA-acetone method and separated by 2D-PAGE (Two-Dimensional Polyacrylamide Gel Electrophoresis). The resulting gels were imaged and visually analyzed to identify stage-specific protein spots. These spots were excised, digested into peptides with trypsin enzyme, and prepared for LC-MS/MS (Liquid Chromatography-Tandem Mass Spectrometry) analysis. A total of 136 distinct proteins were successfully identified from these analyses. Subsequent bioinformatics analysis showed that the majority of these proteins were localized to the cytoplasm and mitochondria. This study demonstrates that the midgut proteome of G. mellonella larvae undergoes significant dynamic changes in a stage-specific manner to meet the increasing metabolic and cellular remodeling demands of growth and metamorphosis. This conclusion is supported by the ontological analysis, which emphasizes the critical functions of these proteins in structural and metabolic processes. Our findings provide proteomic information on insect developmental biology and metabolism and lay the groundwork for understanding this insect's amazing flexibility. Biotechnological applications and pest management plans benefit from these findings.
大蜡蛾(Galleria mellonella)危害养蜂业。它也是宿主-病原体研究中的一个很好的模式生物,可以消化聚合物,使其成为一个有吸引力的生物技术竞争者。2018年,mellonella基因组测序,允许对该物种复杂和动态的生化过程进行更多基于蛋白质组学的研究。在这项研究中,我们根据幼虫的体重对其进行分组,确定了幼虫的体重对应于I、II、III和IV龄阶段,从而表征了幼虫发育对中肠蛋白质组的影响。用tca -丙酮法从解剖的中肠中分离蛋白质,用2D-PAGE(二维聚丙烯酰胺凝胶电泳)分离蛋白质。所得凝胶成像并进行视觉分析,以识别阶段特异性蛋白质斑点。将这些斑点切除,用胰蛋白酶酶切成多肽,制备用于LC-MS/MS(液相色谱-串联质谱)分析。从这些分析中,共成功鉴定出136种不同的蛋白质。随后的生物信息学分析表明,这些蛋白大部分定位于细胞质和线粒体。本研究表明,为满足生长和蜕变对代谢和细胞重塑的需求,大黄蜂幼虫中肠蛋白质组发生了显著的阶段性动态变化。这一结论得到了本体论分析的支持,本体论分析强调了这些蛋白质在结构和代谢过程中的关键功能。我们的发现提供了昆虫发育生物学和代谢的蛋白质组学信息,为理解这种昆虫惊人的灵活性奠定了基础。生物技术应用和病虫害管理计划受益于这些发现。
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引用次数: 0
Evaluation of reference genes for quantitative RT-PCR in Tribolium castaneum following bacterial challenge castaneum细菌侵染后定量RT-PCR内参基因的评价
IF 2.7 2区 农林科学 Q1 ENTOMOLOGY Pub Date : 2025-11-12 DOI: 10.1016/j.jspr.2025.102882
Huixing Ma , Baolin Deng , Qiuying He , Jisheng Liu
Quantitative real-time polymerase chain reaction (qRT-PCR) is a commonly used technique for quantifying gene expression. Selecting appropriate reference genes with relatively stable expression is crucial for data normalization. The red flour beetle (Tribolium castaneum), a globally significant pest affecting stored grains and agricultural products, frequently encounters bacterial pathogens in its environment. This study aims to evaluate the expression stability of candidate reference genes in T. castaneum larvae under infections by Gram-negative bacterium Escherichia coli and Gram-positive bacterium Staphylococcus aureus, thereby enabling reliable subsequent gene expression analysis in this species. The relative expression levels of eight candidate reference genes (ACTB, CAD, RpL13A, RpL32, RpS3, RpS6, RpS18, and Syx1A) across different tissues post-infection were measured by qRT-PCR. Comprehensive evaluation of the expression stability of these candidate reference genes was conducted employing the algorithms of ΔCq, geNorm, NormFinder, and BestKeeper. Our results identified RpS3 as the most stable reference gene in the non-infected control group, while RpL32 and RpL13A demonstrated higher expression stability. Under bacterial infections, RpS3 remained the most stably expressed gene, with RpL32, and RpL13A showing relatively high stability. Notably, RpL32 exhibited good expression stability during S. aureus infection. Therefore, RpS3 and RpL32 are recommended as reference genes for qRT-PCR analysis in T. castaneum larvae following bacterial infections, while RpL13A stands as a reliable alternative.
定量实时聚合酶链反应(qRT-PCR)是一种常用的基因表达定量技术。选择合适且表达相对稳定的内参基因是数据归一化的关键。红粉甲虫(Tribolium castaneum)是一种影响储存谷物和农产品的全球重要害虫,它经常在其环境中遇到细菌病原体。本研究旨在评估候选内参基因在革兰氏阴性菌大肠杆菌和革兰氏阳性菌金黄色葡萄球菌感染下castaneum幼虫中的表达稳定性,从而为后续该物种的基因表达分析提供可靠依据。采用qRT-PCR检测感染后不同组织中8个候选内参基因(ACTB、CAD、RpL13A、RpL32、RpS3、RpS6、RpS18、Syx1A)的相对表达量。采用ΔCq、geNorm、NormFinder、BestKeeper等算法对候选内参基因的表达稳定性进行综合评价。我们的研究结果表明,在未感染的对照组中,RpS3是最稳定的内参基因,而RpL32和RpL13A表现出更高的表达稳定性。在细菌感染下,RpS3是最稳定表达的基因,RpL32和RpL13A表现出相对较高的稳定性。值得注意的是,RpL32在金黄色葡萄球菌感染期间表现出良好的表达稳定性。因此,推荐RpS3和RpL32作为细菌感染后castaneum幼虫qRT-PCR分析的内参基因,而RpL13A是可靠的替代基因。
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引用次数: 0
Impact of modified atmosphere on two mite species Glycyphagus domesticus (Astigmatina: Glycyphagidae) and Caloglyphus oudemansi (Astigmatina: Acaridae) infesting stored wheat grains 改良空气对小麦储粮中两种螨的影响(英文:glycyhaidae
IF 2.7 2区 农林科学 Q1 ENTOMOLOGY Pub Date : 2025-11-11 DOI: 10.1016/j.jspr.2025.102877
Rania Atef , Sayeda S. Ahmed , Reham Abo-Shnaf , Morad F. Hassan
Stored grains are often infested by mites like Glycyphagus domesticus and Caloglyphus oudemansi, which compromise food quality and safety. This study evaluated the effectiveness of modified atmospheres enriched with CO2 or N2 against these mite species. Different concentrations of CO2 (20 %, 40 %, and 60 %) and N2 (97 % and 98 %) were tested for various exposure periods. Results showed that mortality rates increased with increase of exposure time and concentration. G. domesticus exhibited greater susceptibility than C. oudemansi, achieving 100 % egg mortality after 7 days at 60 % CO2 and 98 % N2, whereas C. oudemansi eggs reached a maximum mortality of 70 % after 12 days under the same conditions. Adults of both species were more susceptible than immature stages. For large-scale applications, GM3 (60 % CO2) and GM5 (98 % N2) treatments, with LT90 values, significantly reduced mite populations and weight loss in wheat grains over two months of storage compared to controls. In conclusion, these findings suggest that modified atmosphere is a viable method for controlling mites in stored grains. The use of 60 % CO2 or 98 % N2 can be recommended as a promising solution for managing G. domesticus and C. oudemansi infestations in stored wheat grains. This approach can contributing to reduced losses and improved food quality.
储存的谷物通常会受到像家养糖蚜和欧德曼糖蚜这样的螨虫的侵害,从而影响食品的质量和安全。本研究评价了富集CO2或N2的改良大气环境对这些螨种的防治效果。在不同的暴露时间测试了不同浓度的CO2(20%、40%和60%)和N2(97%和98%)。结果表明,随着暴露时间和暴露浓度的增加,死亡率呈上升趋势。在60% CO2和98% N2条件下,家蝇卵7 d后死亡率为100%,而在相同条件下,家蝇卵12 d后死亡率最高为70%。这两个物种的成虫都比未成熟的幼虫更易受感染。在大规模应用中,与对照相比,LT90值的GM3 (60% CO2)和GM5 (98% N2)处理在两个月的储存期间显著减少了小麦籽粒的螨虫数量和重量损失。综上所述,气调是一种有效的防螨方法。使用60%的CO2或98%的N2可以作为一种有希望的解决方案来管理小麦储粮中的家蝇和乌德曼弧菌侵染。这种方法有助于减少损失和提高食品质量。
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引用次数: 0
Atmospheric cold plasma exposure of cowpea weevils: Mortality kinetics of various life stages 大气冷等离子体暴露豇豆象鼻虫:不同生命阶段的死亡动力学
IF 2.7 2区 农林科学 Q1 ENTOMOLOGY Pub Date : 2025-11-11 DOI: 10.1016/j.jspr.2025.102878
Snehasis Chakraborty , Harshavardhan Reddy Settipalli , Bhadriraju Subramanyam, Kaliramesh Siliveru
The cowpea weevil, Callosobruchus maculatus (F.), infests stored cowpeas (Vigna unguiculata (L.) Walp.) and causes substantial postharvest losses. This study investigated atmospheric cold plasma (ACP) as non-thermal and non-chemical disinfestation method for all life stages of C. maculatus in cowpea seeds. ACP treatments were applied using a multipin-to-plate reactor under ambient air at voltages (Vrms) ranging from 5 to 25 kV and exposure times of 1–20 min. Five developmental stages (eggs, young and mature larvae, pupae, and adults)were individually exposed to ACP treatments, with survival assessed after 28 d. Mortality kinetics were modeled using Weibull and log-linear equations. Sensitivity indices and mortality parameters were calculated to evaluate stage-specific susceptibility. Adults were most susceptible, while pupae were least susceptible. The Weibull model showed a better fit (r2 > 0.85) and revealed mortality of each life stage to be non-linear. β-values deviated from 1, indicating survival curve shapes: pupae (β = 1.1) and adults (β = 1.45) exhibited shouldering trends, whereas eggs (β = 0.85) showed tailing trends. Scale parameters (δ) ranged from 3.9 min in adults at 5 kV to 223.2 min in pupae at 5 kV, highlighting significant stage-specific susceptibility. The pupae had the highest voltage resistance (RV = 162.7 V-1), requiring longer exposure at higher voltages. Complete mortality was experimentally validated at 25 kV for 15.8 min, which matched the lethal time for 99 % mortality (LT99) as predicted by the Weibull model. The optimized ACP treatment preserved seed quality, such as color profile (L∗, a∗, b∗), density, moisture, and germination potential. This evaluation provides a practical and scalable ACP-based disinfestation strategy for stored cowpeas, combining efficacy without compromising safety and quality.
豇豆象鼻虫Callosobruchus maculatus (F.)危害储藏豇豆(Vigna unguiculata (L.))。),并造成大量的收获后损失。研究了常压冷等离子体(ACP)对豇豆种子中斑曲霉(C. maculatus)全生命阶段的非热非化学除虫方法。ACP处理使用多针对板反应器,在环境空气中进行,电压范围为5至25 kV,暴露时间为1-20分钟。五个发育阶段(卵、幼幼虫和成熟幼虫、蛹和成虫)分别暴露于ACP处理下,28 d后评估存活率。死亡率动力学采用威布尔方程和对数线性方程建模。计算敏感性指数和死亡率参数以评估分期特异性易感性。成虫最易感染,蛹最不容易感染。威布尔模型拟合较好(r2 > 0.85),显示各生命阶段死亡率呈非线性。β值偏离1,表明蛹(β = 1.1)和成虫(β = 1.45)呈肩肩趋势,卵(β = 0.85)呈尾尾趋势。尺度参数(δ)从5 kV下成虫的3.9分钟到5 kV下蛹的223.2分钟不等,突出了显著的阶段特异性敏感性。蛹具有最高的耐压性(RV = 162.7 V-1),在较高的电压下需要较长的暴露时间。实验验证了在25 kV下15.8 min的完全死亡时间,符合Weibull模型预测的99%死亡率(LT99)的致死时间。优化后的ACP处理对种子的色型(L∗,a∗,b∗)、密度、水分和发芽势等品质均有较好的影响。该评价为储存豇豆提供了一种实用且可扩展的基于acp的除虫策略,既有效又不影响安全性和质量。
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引用次数: 0
Spatial and seasonal performance of Blattisocius tarsalis in stored grain environments 储粮环境中tarsalis的空间与季节特性
IF 2.7 2区 农林科学 Q1 ENTOMOLOGY Pub Date : 2025-11-11 DOI: 10.1016/j.jspr.2025.102884
Julia Parsons , José Miguel Campos-Rivela , Núria Agustí , María Teresa Martínez Ferrer , Meriem Dahmane , Marta da Silva Lopes , Jordi Riudavets
In large-scale grain storage facilities, various pests can infest stored grain causing significant damage. The predatory mite Blattisocius tarsalis has shown robust dispersal abilities in controlled environments, along with an effective capacity to prey on the eggs of common storage pests. However, its maximum dispersal potential and efficacy in suppressing pest populations under realistic storage conditions remain poorly understood. Furthermore, the impact of temperature fluctuations on its performance has yet to be fully clarified. In this study, two sets of experiments were conducted, one during the summer and the other during the early spring, to evaluate the dispersal efficacy of B. tarsalis released in a large pile of paddy rice. To assess both dispersal and predatory efficacy, eggs of the common storage pest Plodia interpunctella were used as bait. These were placed at varying depths, directions, and distances, up to 4 m from the release point. Our results demonstrate that B. tarsalis can disperse as far as 4 m and effectively control pest populations, irrespective of seasonal conditions. The mites also exhibited a marked preference for moving into the grain mass and away from light sources. Although this study focused on P. interpunctella, which lays eggs on the grain surface, the ability of B. tarsalis to penetrate the grain pile suggests it may also prey on other pest species that oviposit deeper within the grain mass. These findings underscore the potential of B. tarsalis as a sustainable and effective biological control agent in stored-product pest management.
在大型储粮设施中,储粮会受到各种害虫的侵扰,造成重大损失。捕食性螨tarsalis在受控环境中表现出强大的扩散能力,以及捕食常见储存害虫卵的有效能力。然而,在实际储存条件下,其最大扩散潜力和抑制害虫种群的功效仍然知之甚少。此外,温度波动对其性能的影响尚未得到充分澄清。本研究采用夏季和早春两组试验,对大面积水稻中释放的tarsalis的扩散效果进行了评价。以常见储存性害虫斑间Plodia interpunctella卵为饵,评价其扩散和捕食效果。这些装置被放置在不同的深度、方向和距离上,距离释放点最远可达4米。结果表明,无论季节条件如何,tarsalis都能有效地控制害虫种群,分散距离可达4 m。螨虫还表现出明显的偏好进入颗粒团和远离光源。虽然这项研究的重点是在谷物表面产卵的点间疟原虫,但tarsalis穿透谷物堆的能力表明,它也可能捕食在谷物堆深处产卵的其他害虫。这些发现强调了tarsalis作为一种可持续和有效的生物防治剂在储存品害虫管理中的潜力。
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引用次数: 0
Efficacy of putrescine treatments in preserving antioxidant stability and postharvest quality of mandarins during cold storage 腐胺处理对柑桔冷藏抗氧化稳定性及采后品质的影响
IF 2.7 2区 农林科学 Q1 ENTOMOLOGY Pub Date : 2025-11-11 DOI: 10.1016/j.jspr.2025.102886
Manpreet Kumar , Anil Kumar , Kashish Bajaj , Parmpal Singh Gill , Sukhjit Kaur Jawandha , Anil Sangwan
Mandarins (Citrus reticulata) are highly perishable fruits that undergo rapid physiological changes after harvest, including ripening, senescence, and tissue degradation, which compromise their quality and reduce storage life. The present study evaluated the efficacy of postharvest putrescine (PUT) dips on antioxidant stability and nutritive quality of mandarins during cold storage. Fruits were dipped in various concentrations of putrescine (1; 2; 3 mM) and stored at 5–6 °C for 75 days. Antioxidant enzyme activities: superoxide dismutase (SOD), peroxidase (POD), phenylalanine ammonia lyase (PAL) and catalase (CAT) were monitored along with oxidative markers, biochemical and physical quality parameters. By the end of storage, putrescine (3 mM) was most effective, maintaining significantly higher SOD (31.20 U min−1 g−1), CAT (46.58 U min−1 g−1), POD (33.14 U min−1 g−1), and PAL (239.40 U h−1 g−1) activities and reducing malondialdehyde (1.26 mmol g−1) accumulation. PUT treatments also preserved higher levels of ascorbic acid (216.14 mg L−1), flavonoids (0.18 mg g−1), total antioxidants, and titratable acidity (0.71 %) compared with control fruits. Furthermore, treated fruits exhibited lower weight loss (7.35 %), reduced decay incidence (8.97 %), and prolonged shelf life. Correlation analysis showed that flavonoids, and antioxidant activity were positively associated, whereas lipid peroxidation, weight loss, and decay were negatively related to nutritional quality. Principal component analysis separated treated and control fruits, with 2–3 mM putrescine closely associated with antioxidant traits, while controls aligned with deterioration parameters. These findings demonstrated that postharvest putrescine dips, in combination with low temperature storage, can effectively maintain the quality and extend the shelf life of Kinnow mandarins.
柑桔(Citrus reticulata)是一种高度易腐的水果,在收获后会经历快速的生理变化,包括成熟、衰老和组织降解,这些变化会影响其质量,降低其储存寿命。研究了采后腐胺(PUT)对柑桔冷藏过程中抗氧化稳定性和营养品质的影响。将水果浸泡在不同浓度的腐胺(1、2、3 mM)中,在5-6℃下保存75天。检测抗氧化酶活性:超氧化物歧化酶(SOD)、过氧化物酶(POD)、苯丙氨酸解氨酶(PAL)和过氧化氢酶(CAT)以及氧化标志物、生化和物理品质参数。贮藏结束时,腐胺(3 mM)最有效,维持了较高的SOD (31.20 U min−1 g−1)、CAT (46.58 U min−1 g−1)、POD (33.14 U min−1 g−1)和PAL (239.40 U h−1 g−1)活性,降低了丙二醛(1.26 mmol g−1)的积累。PUT处理的抗坏血酸(216.14 mg L−1)、类黄酮(0.18 mg g−1)、总抗氧化剂和可滴定酸度(0.71%)的含量也高于对照。此外,处理过的水果表现出较低的失重(7.35%)、较低的腐烂率(8.97%)和较长的保质期。相关分析表明,黄酮类化合物与抗氧化活性呈正相关,而脂质过氧化、体重减轻和腐烂与营养品质呈负相关。主成分分析表明,2 ~ 3 mM腐胺与抗氧化性状密切相关,而对照与变质参数一致。综上所述,采后腐胺浸渍与低温贮藏相结合,可以有效地保持金诺柑的品质,延长金诺柑的保质期。
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引用次数: 0
Chemical composition and bioefficacy of Pimenta dioica, Cinnamomum verum, and Eucalyptus globulus essential oils against stored-product beetles 洋红、肉桂和蓝桉精油对储藏品甲虫的化学成分及生物功效研究
IF 2.7 2区 农林科学 Q1 ENTOMOLOGY Pub Date : 2025-11-09 DOI: 10.1016/j.jspr.2025.102883
Rajashekar Yallappa , Arunima V. Shine , Aparana Raj , Krishnaiah H. Eraiah , Chikkarasanahalli S. Vivek Babu
The study evaluates the chemical composition and insecticidal properties of essential oils (EOs) from Pimenta dioica, Cinnamomum verum, and Eucalyptus globulus as sustainable alternatives to chemical fumigants for stored grain protection. Gas Chromatography-Mass Spectrometry (GC-MS) analysis identified key compounds: 3-Allyl-6-methoxyphenol, tris(tert-butyldimethylsilyloxy)arsane, and limonene in P. dioica oil; methyl eugenol and cinnamyl ester in C. verum oil; and eucalyptol and α-pinene in E. globulus oil. These bioactive components are linked to their insecticidal efficacy. Fumigant activity was assessed, revealing LC50 values after 24 h of exposure for S. oryzae as 39.9, 147.8, and 38.2 μL L−1, and for T. castaneum as 24.9, 74.9, and 45.3 μL L−1, respectively. Among the EOs, E. globulus demonstrated the highest fumigant toxicity, achieving 100 % mortality at 425 μL/L within 72 h. Contact toxicity tests showed LD50 values of 0.69, 0.31, and 0.88 μL/cm2 for S. oryzae, and 1.09, 0.61, and 1.58 μL/cm2 for T. castaneum after 24 h, indicating moderate effectiveness. The findings underscore the potential of E. globulus as a strong fumigant and C. verum for contact toxicity, attributed to compounds like eucalyptol and methyl eugenol, respectively. These results highlight the potential of these EOs, particularly E. globulus, as eco-friendly alternatives for managing S. oryzae and T. castaneum infestations in stored grains, reducing reliance on synthetic chemical fumigants.
本研究评估了菊芋(Pimenta dioica)、肉桂(Cinnamomum verum)和蓝桉(Eucalyptus globulus)精油的化学成分和杀虫性能,作为化学熏蒸剂对储粮保护的可持续替代品。气相色谱-质谱(GC-MS)分析鉴定出主要化合物:3-烯丙基-6-甲氧基苯酚、三(叔丁基二甲基硅氧基)芳烷和柠檬烯;verum油中的甲基丁香酚和肉桂酯;桉树油和α-蒎烯。这些生物活性成分与其杀虫功效有关。熏蒸活性测定结果显示,暴露24 h后,稻瘟病菌的LC50分别为39.9、147.8和38.2 μL L−1,castaneum的LC50分别为24.9、74.9和45.3 μL L−1。其中,球芽孢杆菌的熏蒸毒性最高,在425 μL/L的熏蒸作用下,72 h内的致死率为100%。接触毒性试验结果显示,24 h后,稻瘟病菌的LD50值分别为0.69、0.31和0.88 μL/cm2, castaneum的LD50值分别为1.09、0.61和1.58 μL/cm2,表明熏蒸效果中等。这些发现强调了球芽孢杆菌作为一种强熏蒸剂和C. verum的接触毒性的潜力,分别归因于桉树酚和甲基丁香酚等化合物。这些结果突出了这些EOs的潜力,特别是E. globulus,作为管理储藏谷物中稻瘟病菌和castaneum侵染的环保替代品,减少了对合成化学熏蒸剂的依赖。
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引用次数: 0
Preferences of four stored product insects to different host grains and their influence on quality 4种储粮昆虫对不同寄主谷物的偏好及其对品质的影响
IF 2.7 2区 农林科学 Q1 ENTOMOLOGY Pub Date : 2025-11-08 DOI: 10.1016/j.jspr.2025.102876
Ali Yılmaz, Mustafa Bayram
Stored cereals and legumes are vital components of the human diet and play a critical role in global food security. However, these commodities are highly susceptible to losses during storage, primarily due to infestations by various insect species. Understanding the behaviour and biochemical impact of these pests is essential for effective postharvest management. This study investigated the activity patterns and effects of Oryzaephilus surinamensis (Linnaeus), Rhyzopertha dominica (Fabricius), Sitophilus granarius (Linnaeus), and Tribolium castaneum (Herbst) on wheat, bulgur (as processed wheat grain), corn, and chickpea at different times of day (morning, noon, and evening). Sitophilus granarius (Linnaeus) showed a strong preference for chickpea, with populations of 8.50 ± 2.12 (morning), 8.00 ± 4.24 (noon), and 8.50 ± 3.54 (evening) insects. T. castaneum activity peaked in the evening on wheat (4.00 ± 1.41) and bulgur (2.00 ± 0.00), but it was nearly absent in chickpea (1.00 ± 0.00 evening) and absent in corn (0.00 ± 0.00 morning) Rhyzopertha dominica (Fabricius) activity slightly increased in corn at noon (3.50 ± 0.71) and bulgur in the evening (3.50 ± 0.71), with minimal activity on chickpea in the morning (0.00 ± 0.00). Oryzaephilus surinamensis (Linnaeus) exhibited very low preference for chickpea (0.50 ± 0.71). Chemical analyses revealed that Tribolium castaneum (Herbst) caused the greatest starch losses in wheat (50.32 ± 2.93 %, wb), chickpea (45.29 ± 1.41 %, wb), and corn (46.29 ± 0.36 %, wb), and markedly increased TBARS levels in wheat (0.011 ± 0.002 → 0.043 ± 0.004 mg MDA/kg) and corn (0.017 ± 0.00 → 0.040 ± 0.028 mg MDA/kg). Oryzaephilus surinamensis (Linnaeus) reduced wheat protein by 51.2 % (16.81 ± 0.25 → 9.72 ± 0.87 %, wb) and chickpea starch by 17.9 % (20.66 ± 1.42 → 16.96 ± 0.45 %, wb). Sitophilus granarius (Linnaeus) decreased corn protein by 29.1 % (13.57 ± 1.10 → 9.63 ± 1.73 %, wb) and chickpea moisture by 25.8 % (12.43 ± 0.63 → 9.22 ± 0.25 %, wb). FTIR analyses further highlighted that Tribolium castaneum (Herbst) caused severe lipid oxidation (2928.5 cm−1) and protein degradation (1646.7 cm−1) in wheat and chickpea, while Sitophilus granarius (Linnaeus) influenced corn by stabilizing chitin–glucan complexes (1076.1 cm−1). Rhyzopertha dominica (Fabricius) altered bulgur through triglyceride esterification (1743.2 cm−1) and chickpea via lipid peroxidation (1336 cm−1), whereas Oryzaephilus surinamensis (Linnaeus) exhibited comparatively limited biochemical effects, collectively demonstrating that each insect species exerts distinct and significant impacts on grain composition, highlighting the urgent need for targeted and effective pest management strategies to safeguard the quality and nutritional value of stored cereals.
储存谷物和豆类是人类饮食的重要组成部分,在全球粮食安全中发挥着关键作用。然而,这些商品在储存期间极易受到损失,主要是由于各种昆虫的侵扰。了解这些害虫的行为和生化影响对有效的采后管理至关重要。研究了不同时段(早、中、晚)稻瘟病菌(Oryzaephilus sulinamensis, linnaus Rhyzopertha dominica, linnaus granophilus, Tribolium castaneum, Herbst)对小麦、干小麦(加工小麦)、玉米和鹰嘴豆的活性规律和影响。粗象象对鹰嘴豆有强烈的偏好,其种群数量分别为8.50±2.12(早晨)、8.00±4.24(中午)和8.50±3.54(晚上)。castaneum在小麦(4.00±1.41)和干小麦(2.00±0.00)上的活性在晚上达到峰值,而在鹰嘴豆(1.00±0.00)和玉米(0.00±0.00)上几乎不存在,在玉米(3.50±0.71)和干小麦(3.50±0.71)上的活性在中午(3.50±0.71)和晚上(3.50±0.71)上略有增加,在鹰嘴豆(0.00±0.00)上活性最小。苏里南稻霉(Linnaeus)对鹰嘴豆的偏好度极低(0.50±0.71)。化学分析结果表明,在小麦(50.32±2.93%,wb)、鹰嘴豆(45.29±1.41%,wb)和玉米(46.29±0.36%,wb)中,Tribolium castaneum (Herbst)造成的淀粉损失最大,显著提高了小麦(0.011±0.002→0.043±0.004 mg MDA/kg)和玉米(0.017±0.00→0.040±0.028 mg MDA/kg)的TBARS水平。Oryzaephilus sulinamensis (Linnaeus)减少小麦蛋白51.2%(16.81±0.25→9.72±0.87%,wb),鹰嘴豆淀粉17.9%(20.66±1.42→16.96±0.45%,wb)。玉米蛋白含量降低29.1%(13.57±1.10→9.63±1.73%,wb),鹰嘴豆水分含量降低25.8%(12.43±0.63→9.22±0.25%,wb)。FTIR分析进一步强调,Tribolium castaneum (Herbst)在小麦和鹰嘴豆中引起严重的脂质氧化(2928.5 cm−1)和蛋白质降解(1646.7 cm−1),而Sitophilus granarius (Linnaeus)通过稳定几丁质-葡聚糖复合物(1076.1 cm−1)影响玉米。Rhyzopertha dominica (Fabricius)通过甘油三酯酯化(1743.2 cm−1)改变了bulgur,鹰嘴豆(1336 cm−1)通过脂质过氧化(1336 cm−1)改变了鹰嘴豆,而Oryzaephilus surinamensis (Linnaeus)表现出相对有限的生化效应,共同表明每种昆虫对谷物组成都有不同且显著的影响。强调迫切需要有针对性和有效的有害生物管理战略,以保障储存谷物的质量和营养价值。
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引用次数: 0
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Journal of Stored Products Research
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