This study sought to investigate the efficacy of hermetic silos coupled with controlled atmospheres to extend quality of seeds. This study evaluated the influence of low partial oxygen, high carbon dioxide pressures and four storage temperatures (10, 15, 20, and 25 °C), on the physiological soybean quality seeds and subsequent crop yield. For the 2020/2021 season, soybeans seeds of NA 5909 RR cultivar, were stored for the conditions: {1} ambient, {2} 1.0 kPa O2 + 0.04 kPa CO2, and {3} 1.0 kPa O2 + 15.0 kPa CO2 for 6 months at 10, 15, 20, and 25 °C. In the 2021/2022 season, the conditions tested were: {1} ambient, {2} 1.0 kPa O2 + 0.0 kPa CO2, {3} 3.0 kPa O2 + 0.0 kPa CO2, and {4} 5.0 kPa O2 + 0.0 kPa CO2 at three temperatures (10, 15, and 20 °C). Moisture content, electrical conductivity, standard germination tests, seedling length and dry mass, hydrogen peroxide content, superoxide dismutase and guaiacol peroxidase activities were evaluated. After storage, the seeds were evaluated for field emergence, plant height, leaf area index, and yield. In the first and second year of evaluation, seeds stored at 10 °C under 1.0 kPa O2 show superior physiological quality in the assessed variables. In general, these condition (1.0 kPa O2 + 10 °C) increased field productivity, ranging from 82.0 to 421.0 kg ha−1 compared to ambient conditions and 268.7–978.0 kg ha−1 compared to seeds stored under other temperature conditions. High carbon dioxide pressures did not provide any additive benefit to low partial oxygen pressures in preserving the seeds physiological quality after storage. After six months of storage, all CA conditions maintained superior physiological quality of seeds, guaranteeing greater productivity, especially at low temperatures of 10 and 15 °C.
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