Journal of Stored Products Research最新文献_第3页

Two peas in a pod: Efficacy of S-methoprene in combination with piperonyl butoxide for the control of different strains of the lesser grain borer, Rhyzopertha dominica (F.) (Coleoptera: Bostrychidae) 一荚两豆：s -甲氧二烯与胡椒酰丁二醇联用防治小螟虫不同品系的效果(鞘翅目:Bostrychidae)

IF 2.7 2区农林科学 Q1 ENTOMOLOGY

Journal of Stored Products Research

Pub Date : 2026-01-10 DOI: 10.1016/j.jspr.2026.102949

Maria Κ. Sakka , Graham Moores , Marina Gourgouta , Mariastela Vrontaki , Christos G. Athanassiou

The lesser grain borer, Rhyzopertha dominica (F.) (Coleoptera: Bostrychidae), is one of the most destructive pests of stored cereals worldwide, and resistance to insect growth regulators such as S-methoprene has been documented in several populations. In the present study, we evaluated the effects of S-methoprene alone and in combination with the synergist piperonyl butoxide (PBO) against susceptible (QRD14) and resistant (QRD551) strains of R. dominica. Bioassays were conducted on wheat treated with two concentrations of S-methoprene (0.1 and 0.3 mg/kg for QRD14; 10 and 30 mg/kg for QRD551 and two concentrations of PBO (0.27 and 1.35 g/kg). Treatments were applied under three exposure scenarios: (i) sequential application of S-methoprene followed by PBO with a 5-h interval, (ii) sequential application of PBO followed by S-methoprene with a 5-h interval, and (iii) simultaneous application of both compounds. Adult mortality was generally low in both strains, not exceeding 30 % even after 21 d, although the combination. In contrast, progeny production was almost completely suppressed in QRD14 across all treatments, while in the QRD551 strain, progeny suppression was dose-dependent and strongest when S-methoprene at 30 mg/kg was combined with PBO at 1.35 g/kg. These results demonstrate that progeny suppression, rather than adult mortality, is the key effect of S-methoprene against R. dominica, and that PBO can enhance its efficacy, particularly in resistant populations.

小纹螟（Rhyzopertha dominica）（鞘翅目：Bostrychidae）是世界上储藏谷物中最具破坏性的害虫之一，并且在一些种群中已记录到对s -甲氧丁二烯等昆虫生长调节剂的抗性。在本研究中，我们评估了s -甲基戊二烯单独和与增效剂胡椒酰丁醇（PBO）联合对多米尼克白僵菌敏感（QRD14）和耐药（QRD551）菌株的作用。采用两种浓度的s -甲基戊二烯（QRD14为0.1和0.3 mg/kg， QRD551为10和30 mg/kg）和两种浓度的PBO（0.27和1.35 g/kg）处理小麦进行生物测定。在三种暴露情景下进行处理：(i)顺序施用s -甲基戊二烯后再施用PBO，间隔5小时；（ii）顺序施用PBO后再施用s -甲基戊二烯，间隔5小时；（iii）同时施用两种化合物。两种菌株的成虫死亡率普遍较低，即使在21天后也不超过30%。相比之下，QRD14在所有处理中几乎完全抑制了后代的产生，而在QRD551菌株中，当30 mg/kg的s -甲氧二烯与1.35 g/kg的PBO联合使用时，后代的抑制是剂量依赖性的，并且最强。这些结果表明，s -甲基戊二烯对多米尼加小蠊的主要作用是抑制后代，而不是导致成虫死亡，而PBO可以增强其药效，特别是在耐药种群中。

{"title":"Two peas in a pod: Efficacy of S-methoprene in combination with piperonyl butoxide for the control of different strains of the lesser grain borer, Rhyzopertha dominica (F.) (Coleoptera: Bostrychidae)","authors":"Maria Κ. Sakka , Graham Moores , Marina Gourgouta , Mariastela Vrontaki , Christos G. Athanassiou","doi":"10.1016/j.jspr.2026.102949","DOIUrl":"10.1016/j.jspr.2026.102949","url":null,"abstract":"<div><div>The lesser grain borer, <em>Rhyzopertha dominica</em> (F.) (Coleoptera: Bostrychidae), is one of the most destructive pests of stored cereals worldwide, and resistance to insect growth regulators such as S-methoprene has been documented in several populations. In the present study, we evaluated the effects of S-methoprene alone and in combination with the synergist piperonyl butoxide (PBO) against susceptible (QRD14) and resistant (QRD551) strains of <em>R. dominica</em>. Bioassays were conducted on wheat treated with two concentrations of S-methoprene (0.1 and 0.3 mg/kg for QRD14; 10 and 30 mg/kg for QRD551 and two concentrations of PBO (0.27 and 1.35 g/kg). Treatments were applied under three exposure scenarios: (i) sequential application of S-methoprene followed by PBO with a 5-h interval, (ii) sequential application of PBO followed by S-methoprene with a 5-h interval, and (iii) simultaneous application of both compounds. Adult mortality was generally low in both strains, not exceeding 30 % even after 21 d, although the combination. In contrast, progeny production was almost completely suppressed in QRD14 across all treatments, while in the QRD551 strain, progeny suppression was dose-dependent and strongest when S-methoprene at 30 mg/kg was combined with PBO at 1.35 g/kg. These results demonstrate that progeny suppression, rather than adult mortality, is the key effect of S-methoprene against <em>R. dominica</em>, and that PBO can enhance its efficacy, particularly in resistant populations.</div></div>","PeriodicalId":17019,"journal":{"name":"Journal of Stored Products Research","volume":"116 ","pages":"Article 102949"},"PeriodicalIF":2.7,"publicationDate":"2026-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145925146","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Non-thermal sterilization of coconut water via double plasma ozonation: Inactivation kinetics and process design 双等离子体臭氧氧化椰子水的非热灭菌：失活动力学和工艺设计

IF 2.7 2区农林科学 Q1 ENTOMOLOGY

Journal of Stored Products Research

Pub Date : 2026-01-08 DOI: 10.1016/j.jspr.2026.102950

David Yudianto , Bella Mellisani , Hanafi , Henny Rochaeny , Nurhasanah , Septilina Melati Sirait , Alvina Nur Aini , Arya Ulilalbab , Andita Sayekti , Ellyas Alga Nainggolan , Klara Urbanova

Non-thermal ozonation using a double plasma system presents a promising alternative to conventional heat-based sterilization for maintaining the quality and microbial safety of coconut water. This study aimed to evaluate the inactivation kinetics of Geobacillus stearothermophilus, Bacillus cereus, and Bacillus subtilis during coconut water ozonation and to develop an optimized process design using kinetic parameters. The ozonation process was conducted with a double plasma generator at airflow rates of 1–4 L min⁻¹, corresponding to ozone concentrations of 209–720 mg L⁻¹. The inactivation kinetics followed first-order behavior, where higher ozone concentrations resulted in steeper inactivation slopes and shorter D-values. The D-values at 720 mg L⁻¹ were 30.19, 21.56, and 29.83 min for G. stearothermophilus, B. cereus, and B. subtilis, respectively, while corresponding Z-values were 4151.30, 2304.14, and 3286.38 mg L⁻¹. An optimization model was developed based on these parameters to predict ozonation time–concentration combinations for 3D, 6D, and 9D inactivation levels. The 9D inactivation line for G. stearothermophilus defined the critical design boundary ensuring total bacterial reduction. The optimized condition, ozonation at 720 mg L⁻¹ for 500 min, achieved theoretical 9-log reductions (99.9999999 %) across all bacteria tested. Despite relatively high energy demands, double plasma ozonation offers a viable non-thermal sterilization approach for extending coconut water shelf life while maintaining sensory quality. Future work should focus on scaling, energy optimization, and renewable power integration to enhance its industrial feasibility.

采用双等离子体系统的非热臭氧化是一种有希望的替代传统热基灭菌的方法，可以保持椰子汁的质量和微生物安全。本研究旨在评价嗜脂热地杆菌、蜡样芽孢杆菌和枯草芽孢杆菌在椰子水臭氧化过程中的失活动力学，并利用动力学参数进行工艺优化设计。臭氧化过程在双等离子体发生器上进行，气流速率为1 ~ 4 L min - 1，臭氧浓度为209 ~ 720 mg L - 1。失活动力学遵循一阶行为，较高的臭氧浓度导致更陡的失活斜率和更短的d值。在720 mg L - 1下，嗜脂嗜热杆菌、蜡样芽孢杆菌和枯草芽孢杆菌的d值分别为30.19、21.56和29.83 min，对应的z值分别为4151.30、2304.14和3286.38 mg L - 1。基于这些参数建立了优化模型，以预测臭氧化时间-浓度组合对3D、6D和9D失活水平的影响。硬脂嗜热杆菌的9D失活线确定了确保细菌总数减少的关键设计边界。优化条件为720 mg L−1臭氧氧化500 min，所有被测细菌的理论上减少量为9对数（99.9999999%）。尽管能量需求相对较高，但双等离子体臭氧氧化提供了一种可行的非热灭菌方法，可以延长椰子汁的保质期，同时保持感官质量。未来的工作应侧重于规模化、能源优化和可再生能源整合，以提高其产业可行性。

{"title":"Non-thermal sterilization of coconut water via double plasma ozonation: Inactivation kinetics and process design","authors":"David Yudianto , Bella Mellisani , Hanafi , Henny Rochaeny , Nurhasanah , Septilina Melati Sirait , Alvina Nur Aini , Arya Ulilalbab , Andita Sayekti , Ellyas Alga Nainggolan , Klara Urbanova","doi":"10.1016/j.jspr.2026.102950","DOIUrl":"10.1016/j.jspr.2026.102950","url":null,"abstract":"<div><div>Non-thermal ozonation using a double plasma system presents a promising alternative to conventional heat-based sterilization for maintaining the quality and microbial safety of coconut water. This study aimed to evaluate the inactivation kinetics of <em>Geobacillus stearothermophilus</em>, <em>Bacillus cereus</em>, and <em>Bacillus subtilis</em> during coconut water ozonation and to develop an optimized process design using kinetic parameters. The ozonation process was conducted with a double plasma generator at airflow rates of 1–4 L min<sup>−1</sup>, corresponding to ozone concentrations of 209–720 mg L<sup>−1</sup>. The inactivation kinetics followed first-order behavior, where higher ozone concentrations resulted in steeper inactivation slopes and shorter D-values. The D-values at 720 mg L<sup>−1</sup> were 30.19, 21.56, and 29.83 min for <em>G. stearothermophilus</em>, <em>B. cereus</em>, and <em>B. subtilis</em>, respectively, while corresponding Z-values were 4151.30, 2304.14, and 3286.38 mg L<sup>−1</sup>. An optimization model was developed based on these parameters to predict ozonation time–concentration combinations for 3D, 6D, and 9D inactivation levels. The 9D inactivation line for <em>G. stearothermophilus</em> defined the critical design boundary ensuring total bacterial reduction. The optimized condition, ozonation at 720 mg L<sup>−1</sup> for 500 min, achieved theoretical 9-log reductions (99.9999999 %) across all bacteria tested. Despite relatively high energy demands, double plasma ozonation offers a viable non-thermal sterilization approach for extending coconut water shelf life while maintaining sensory quality. Future work should focus on scaling, energy optimization, and renewable power integration to enhance its industrial feasibility.</div></div>","PeriodicalId":17019,"journal":{"name":"Journal of Stored Products Research","volume":"116 ","pages":"Article 102950"},"PeriodicalIF":2.7,"publicationDate":"2026-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145925145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synergistic insecticidal mechanism of 2-undecanone and decanal against Tribolium castaneum larvae via dual suppression of GST and FDa pathways 2-十一烷酮和癸烷酮通过双重抑制GST和FDa途径协同杀虫的机制

IF 2.7 2区农林科学 Q1 ENTOMOLOGY

Journal of Stored Products Research

Pub Date : 2026-01-07 DOI: 10.1016/j.jspr.2025.102938

Chunxue You , Yao Yao , Kaidi Dai , Jinming Chen , Yanming Wu , Wenjuan Zhang , Xiaoxue Yu , Chao Jiang , Xiumei Gao

Tribolium castaneum (Herbst) is a major stored-product pest, necessitating safer alternatives targeting its destructive larval stage. This study investigated the synergistic insecticidal effects of 2-undecanone and decanal, two active constituents from Houttuynia cordata essential oil, against T. castaneum larvae and elucidated the underlying mechanisms via quantitative real-time PCR (qRT-PCR), RNA interference (RNAi), histopathology, molecular docking, ELISA, and enzyme activity assays. Contact toxicity assays identified the 5:5 mixture as optimally effective, exhibiting significant synergy (R ≥ 1.5) and the lowest LD₅₀ (14.8 μg/larva). Whereas 2-undecanone alone upregulated farnesol dehydrogenase (FDa), glutathione S-transferase (GST), and cytochrome P450 (CYP6BQ7), the 5:5 mixture significantly suppressed the expression of FDa and GST (>60 %), accompanied by decreased GST enzyme activity and lower juvenile hormone (JH) III levels. RNAi silencing of FDa and GST confirmed their important roles in larval susceptibility. Molecular docking predicted interactions of both compounds with FDa and GST, implying potential cooperative inhibition. Histopathology revealed more severe midgut damage under the mixture than under either compound alone. In conclusion, the synergy arises from coordinated suppression of the GST-mediated detoxification and FDa-linked JH biosynthesis, offering a promising strategy for developing potent, eco-friendly botanical formulations for stored-product pest management.

甘蔗Tribolium castaneum （Herbst）是一种主要的储藏产品害虫，需要更安全的替代品来针对其破坏性的幼虫阶段。本研究通过实时荧光定量PCR （qRT-PCR）、RNA干扰（RNAi）、组织病理学、分子对接、ELISA和酶活性等方法，研究鱼腥草精油中2-十一烷酮和十烷酮两种有效成分对castaneum幼虫的协同杀虫作用，并阐明其作用机制。接触毒性试验表明，5:5的配伍效果最佳，协同效应显著（R≥1.5），LD50最低（14.8 μg/幼虫）。2-十烷酮单独上调法尼醇脱氢酶（FDa）、谷胱甘肽s -转移酶（GST）和细胞色素P450 (CYP6BQ7)，而5:5混合物显著抑制FDa和GST的表达（60%），并伴有GST酶活性降低和幼体激素（JH） III水平降低。FDa和GST的RNAi沉默证实了它们在幼虫易感性中的重要作用。分子对接预测了两种化合物与FDa和GST的相互作用，暗示了潜在的协同抑制作用。组织病理学显示，与单独使用任何一种化合物相比，混合物对中肠的损伤更为严重。综上所述，协同抑制gst介导的解毒和与fda相关的JH生物合成产生了协同作用，为开发有效的生态友好型植物制剂提供了一种有希望的策略，用于储存品有害生物管理。

{"title":"Synergistic insecticidal mechanism of 2-undecanone and decanal against Tribolium castaneum larvae via dual suppression of GST and FDa pathways","authors":"Chunxue You , Yao Yao , Kaidi Dai , Jinming Chen , Yanming Wu , Wenjuan Zhang , Xiaoxue Yu , Chao Jiang , Xiumei Gao","doi":"10.1016/j.jspr.2025.102938","DOIUrl":"10.1016/j.jspr.2025.102938","url":null,"abstract":"<div><div><em>Tribolium castaneum</em> (Herbst) is a major stored-product pest, necessitating safer alternatives targeting its destructive larval stage. This study investigated the synergistic insecticidal effects of 2-undecanone and decanal, two active constituents from <em>Houttuynia cordata</em> essential oil, against <em>T. castaneum</em> larvae and elucidated the underlying mechanisms via quantitative real-time PCR (qRT-PCR), RNA interference (RNAi), histopathology, molecular docking, ELISA, and enzyme activity assays. Contact toxicity assays identified the 5:5 mixture as optimally effective, exhibiting significant synergy (R ≥ 1.5) and the lowest LD<sub>50</sub> (14.8 μg/larva). Whereas 2-undecanone alone upregulated farnesol dehydrogenase (<em>FDa</em>), glutathione S-transferase (<em>GST</em>), and cytochrome P450 (<em>CYP6BQ7</em>), the 5:5 mixture significantly suppressed the expression of <em>FDa</em> and <em>GST</em> (>60 %), accompanied by decreased GST enzyme activity and lower juvenile hormone (JH) III levels. RNAi silencing of <em>FDa</em> and <em>GST</em> confirmed their important roles in larval susceptibility. Molecular docking predicted interactions of both compounds with FDa and GST, implying potential cooperative inhibition. Histopathology revealed more severe midgut damage under the mixture than under either compound alone. In conclusion, the synergy arises from coordinated suppression of the GST-mediated detoxification and FDa-linked JH biosynthesis, offering a promising strategy for developing potent, eco-friendly botanical formulations for stored-product pest management.</div></div>","PeriodicalId":17019,"journal":{"name":"Journal of Stored Products Research","volume":"116 ","pages":"Article 102938"},"PeriodicalIF":2.7,"publicationDate":"2026-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145925147","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A multi-defect detection framework for sweet potato based on feature fusion and adaptive attention under complex postharvest conditions 复杂采后条件下基于特征融合和自适应关注的甘薯多缺陷检测框架

IF 2.7 2区农林科学 Q1 ENTOMOLOGY

Journal of Stored Products Research

Pub Date : 2026-01-02 DOI: 10.1016/j.jspr.2025.102937

Xinyu Guo , Jian Zhang , Haitao Xiong , Tao Zhang , Ranbing Yang , Xufeng Wang

Sweet potato, as a major global economic and food crop, often suffers from surface defects due to its complex growth environment and harvesting processes. The long-term mixing of healthy and defective sweet potatoes during storage accelerates the physiological deterioration. This significantly reduces their edible quality and market value. To address this issue, this research presents SP-YOLOv11, a high-performance defect detection method designed to identify and grade four surface conditions of sweet potatoes in complex harvesting environments, including intact skin, minor defects, moderate defects, and severe defects. Specifically, first, a CPPA module is introduced, which employs a multi-branch feature extraction strategy to enhance the model's feature representation and detail-capturing ability. Next, an FDPN module is constructed, which, through multi-scale fusion and feature diffusion mechanisms, improves the adaptability to defects of different scales. Finally, an MSE-Detect module is introduced to focus on the key defect areas of sweet potatoes, further enhancing robustness in agricultural scenarios. To validate the effectiveness, eight ablation experiments are conducted. The results show that SP-YOLOv11 achieves the highest detection accuracy for sweet potato defects, with mAP@0.5 and mAP@0.5:0.95 reaching 98.50 % and 85.60 %, respectively. This research effectively overcomes the challenges of low efficiency, poor accuracy, and high damage rates in traditional defect detection methods. It significantly reduces the spread of diseases and physiological deterioration, while enhancing the post-harvest freshness retention and storage quality of sweet potatoes. The proposed approach provides technical support for their commercial processing and intelligent grading.

甘薯作为全球主要的经济和粮食作物，由于其复杂的生长环境和收获过程，经常出现表面缺陷。健康红薯与缺陷红薯在贮藏过程中长期混合，加速了红薯的生理变质。这大大降低了它们的食用质量和市场价值。为了解决这一问题，本研究提出了一种高性能缺陷检测方法SP-YOLOv11，旨在识别复杂收获环境下红薯的四种表面状况，包括完整的果皮、轻微缺陷、中度缺陷和严重缺陷，并对其进行分级。具体而言，首先介绍了一个CPPA模块，该模块采用多分支特征提取策略来增强模型的特征表示和细节捕获能力；其次，构建FDPN模块，通过多尺度融合和特征扩散机制，提高对不同尺度缺陷的适应性。最后，引入mse检测模块，重点关注红薯的关键缺陷区域，进一步增强农业场景下的鲁棒性。为了验证该方法的有效性，进行了8次烧蚀实验。结果表明，SP-YOLOv11对红薯缺陷的检测准确率最高，mAP@0.5和mAP@0.5:0.95分别达到98.50%和85.60%。该研究有效地克服了传统缺陷检测方法效率低、精度差、损伤率高等缺点。显著降低了病害的传播和生理变质，提高了红薯采后的保鲜度和贮藏品质。该方法为其商业化加工和智能化分级提供了技术支持。

{"title":"A multi-defect detection framework for sweet potato based on feature fusion and adaptive attention under complex postharvest conditions","authors":"Xinyu Guo , Jian Zhang , Haitao Xiong , Tao Zhang , Ranbing Yang , Xufeng Wang","doi":"10.1016/j.jspr.2025.102937","DOIUrl":"10.1016/j.jspr.2025.102937","url":null,"abstract":"<div><div>Sweet potato, as a major global economic and food crop, often suffers from surface defects due to its complex growth environment and harvesting processes. The long-term mixing of healthy and defective sweet potatoes during storage accelerates the physiological deterioration. This significantly reduces their edible quality and market value. To address this issue, this research presents SP-YOLOv11, a high-performance defect detection method designed to identify and grade four surface conditions of sweet potatoes in complex harvesting environments, including intact skin, minor defects, moderate defects, and severe defects. Specifically, first, a CPPA module is introduced, which employs a multi-branch feature extraction strategy to enhance the model's feature representation and detail-capturing ability. Next, an FDPN module is constructed, which, through multi-scale fusion and feature diffusion mechanisms, improves the adaptability to defects of different scales. Finally, an MSE-Detect module is introduced to focus on the key defect areas of sweet potatoes, further enhancing robustness in agricultural scenarios. To validate the effectiveness, eight ablation experiments are conducted. The results show that SP-YOLOv11 achieves the highest detection accuracy for sweet potato defects, with <em>mAP@</em>0.5 and <em>mAP@</em>0.5:0.95 reaching 98.50 % and 85.60 %, respectively. This research effectively overcomes the challenges of low efficiency, poor accuracy, and high damage rates in traditional defect detection methods. It significantly reduces the spread of diseases and physiological deterioration, while enhancing the post-harvest freshness retention and storage quality of sweet potatoes. The proposed approach provides technical support for their commercial processing and intelligent grading.</div></div>","PeriodicalId":17019,"journal":{"name":"Journal of Stored Products Research","volume":"116 ","pages":"Article 102937"},"PeriodicalIF":2.7,"publicationDate":"2026-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145880499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Encapsulation of Zanthoxylum bungeanum essential oil in a tea saponin-stabilized nanoemulsion for enhanced insecticidal control of Cryptolestes ferrugineus 茶皂素稳定纳米乳包封花椒精油对铁隐蝇的杀虫效果研究

IF 2.7 2区农林科学 Q1 ENTOMOLOGY

Journal of Stored Products Research

Pub Date : 2026-01-02 DOI: 10.1016/j.jspr.2025.102934

Liya Zeng , Weihan Ding , Wenxiang Duan , Zhe Wang

To overcome the inherent limitations of Zanthoxylum bungeanum essential oil (ZBEO), such as high volatility and poor water solubility, a potentially green nanoemulsion was developed using tea saponin (TS) as a natural co-stabilizer with Tween 80 for the enhanced insecticidal control of Cryptolestes ferrugineus. The optimized formulation (ZBEO: Tween 80:TS = 2:1:1, w/w) exhibited a small droplet size (165.49 ± 2.98 nm), low polydispersity index (0.29 ± 0.02), and high negative zeta potential (−34.79 ± 3.02 mV). This stability originated from a synergistic mechanism between Tween 80 and TS: Tween 80 provided steric hindrance, while TS contributed electrostatic repulsion at the oil-water interface. Bioassays demonstrated that nanoencapsulation significantly enhanced the insecticidal efficacy of ZBEO, reducing the LD₅₀ for contact toxicity and the LC₅₀ for fumigation toxicity by 25 % and 41 %, respectively. Notably, after 90 days of storage, the nanoemulsion retained substantial bioactivity, with only a minifmal increase in LC₅₀ values, and remained significantly more effective than freshly prepared, unformulated ZBEO. The enhanced performance could be attributed to improved cuticular penetration and controlled release enabled by the TS-based stabilization system. This work confirms TS as an efficient natural co-stabilizer and presents the ZBEO nanoemulsion as a promising, high-efficacy candidate based on natural components for stored-grain pest management.

为了克服花椒精油挥发性高、水溶性差等固有的局限性，以茶皂素（TS）为天然助稳定剂，与Tween 80共同制备了一种绿色纳米乳，以增强对铁隐蝽的杀虫效果。优化后的配方（ZBEO: Tween 80:TS = 2:1:1, w/w）液滴尺寸小（165.49±2.98 nm），多分散性指数低（0.29±0.02），zeta负电位高（- 34.79±3.02 mV）。这种稳定性源于t80和TS之间的协同机制：t80提供空间位阻，而TS在油水界面提供静电斥力。生物实验表明，纳米包封能显著提高ZBEO的杀虫效果，使其接触毒性LD50和熏蒸毒性LC50分别降低25%和41%。值得注意的是，经过90天的储存，纳米乳保留了大量的生物活性，LC50值只有微小的增加，并且仍然比新鲜制备的未配制的ZBEO更有效。这种增强的性能可能归因于基于ts的稳定系统改善了角质层的渗透和控制释放。这项研究证实了TS是一种高效的天然共稳定剂，并提出了基于天然成分的ZBEO纳米乳作为一种有前途的、高效的储粮害虫治理候选物。

{"title":"Encapsulation of Zanthoxylum bungeanum essential oil in a tea saponin-stabilized nanoemulsion for enhanced insecticidal control of Cryptolestes ferrugineus","authors":"Liya Zeng , Weihan Ding , Wenxiang Duan , Zhe Wang","doi":"10.1016/j.jspr.2025.102934","DOIUrl":"10.1016/j.jspr.2025.102934","url":null,"abstract":"<div><div>To overcome the inherent limitations of <em>Zanthoxylum bungeanum</em> essential oil (ZBEO), such as high volatility and poor water solubility, a potentially green nanoemulsion was developed using tea saponin (TS) as a natural co-stabilizer with Tween 80 for the enhanced insecticidal control of <em>Cryptolestes ferrugineus</em>. The optimized formulation (ZBEO: Tween 80:TS = 2:1:1, w/w) exhibited a small droplet size (165.49 ± 2.98 nm), low polydispersity index (0.29 ± 0.02), and high negative zeta potential (−34.79 ± 3.02 mV). This stability originated from a synergistic mechanism between Tween 80 and TS: Tween 80 provided steric hindrance, while TS contributed electrostatic repulsion at the oil-water interface. Bioassays demonstrated that nanoencapsulation significantly enhanced the insecticidal efficacy of ZBEO, reducing the LD<sub>50</sub> for contact toxicity and the LC<sub>50</sub> for fumigation toxicity by 25 % and 41 %, respectively. Notably, after 90 days of storage, the nanoemulsion retained substantial bioactivity, with only a minifmal increase in LC<sub>50</sub> values, and remained significantly more effective than freshly prepared, unformulated ZBEO. The enhanced performance could be attributed to improved cuticular penetration and controlled release enabled by the TS-based stabilization system. This work confirms TS as an efficient natural co-stabilizer and presents the ZBEO nanoemulsion as a promising, high-efficacy candidate based on natural components for stored-grain pest management.</div></div>","PeriodicalId":17019,"journal":{"name":"Journal of Stored Products Research","volume":"116 ","pages":"Article 102934"},"PeriodicalIF":2.7,"publicationDate":"2026-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145880497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sustainable and regulatory perspectives on stimuli-responsive protein-based films for postharvest food and feed preservation 刺激反应蛋白薄膜在采后食品和饲料保存中的可持续和调控前景

IF 2.7 2区农林科学 Q1 ENTOMOLOGY

Journal of Stored Products Research

Pub Date : 2025-12-31 DOI: 10.1016/j.jspr.2025.102947

Moslem Sabaghi , Mohammad M. Seyedalmoosavi , Sedighe Tavasoli

Stimuli-responsive nutri-packaging has emerged as a promising strategy for preserving the quality, safety, and nutritional integrity of perishable food and feed products. This review focuses on protein-based sustainable packaging materials, highlighting performance enhancement strategies and the design of stimuli-responsive films, including physically, chemically, and biologically responsive systems. These materials are engineered to delay senescence, inhibit microbial growth, and limit nutrient degradation in fruits, vegetables, and compound feeds. Sustainability aspects are addressed through eco-design approaches, valorization of agro-industrial by-products, and Life Cycle Assessment, while the emerging role of Digital Twin technologies is discussed as a tool for simulating and optimizing packaging performance across the life cycle. Relevant regulatory and safety frameworks for food and feed applications are reviewed, along with future perspectives such as responsive modified atmosphere packaging and biosensor integration. Overall, this review provides a multidisciplinary outlook on protein-based stimuli-responsive films aligned with Sustainable Development Goals 3, 9, and 12.

刺激反应型营养包装已经成为一种很有前途的策略，可以保持易腐食品和饲料产品的质量、安全性和营养完整性。本文综述了以蛋白质为基础的可持续包装材料，重点介绍了性能增强策略和刺激反应膜的设计，包括物理，化学和生物反应系统。这些材料被设计用来延缓衰老，抑制微生物生长，限制水果、蔬菜和复合饲料中的营养物质降解。可持续性方面通过生态设计方法，农业工业副产品的价值和生命周期评估来解决，而数字孪生技术的新兴作用作为模拟和优化整个生命周期包装性能的工具进行了讨论。综述了食品和饲料应用的相关监管和安全框架，以及未来的前景，如响应性改性大气包装和生物传感器集成。总体而言，本文综述了与可持续发展目标3、9和12相一致的基于蛋白质的刺激反应电影的多学科前景。

{"title":"Sustainable and regulatory perspectives on stimuli-responsive protein-based films for postharvest food and feed preservation","authors":"Moslem Sabaghi , Mohammad M. Seyedalmoosavi , Sedighe Tavasoli","doi":"10.1016/j.jspr.2025.102947","DOIUrl":"10.1016/j.jspr.2025.102947","url":null,"abstract":"<div><div>Stimuli-responsive nutri-packaging has emerged as a promising strategy for preserving the quality, safety, and nutritional integrity of perishable food and feed products. This review focuses on protein-based sustainable packaging materials, highlighting performance enhancement strategies and the design of stimuli-responsive films, including physically, chemically, and biologically responsive systems. These materials are engineered to delay senescence, inhibit microbial growth, and limit nutrient degradation in fruits, vegetables, and compound feeds. Sustainability aspects are addressed through eco-design approaches, valorization of agro-industrial by-products, and Life Cycle Assessment, while the emerging role of Digital Twin technologies is discussed as a tool for simulating and optimizing packaging performance across the life cycle. Relevant regulatory and safety frameworks for food and feed applications are reviewed, along with future perspectives such as responsive modified atmosphere packaging and biosensor integration. Overall, this review provides a multidisciplinary outlook on protein-based stimuli-responsive films aligned with Sustainable Development Goals 3, 9, and 12.</div></div>","PeriodicalId":17019,"journal":{"name":"Journal of Stored Products Research","volume":"116 ","pages":"Article 102947"},"PeriodicalIF":2.7,"publicationDate":"2025-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145880383","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ultrasound-enhanced ferulic acid treatment modulates starch gel structure to inhibit retrogradation in rice starch during storage 超声增强阿魏酸处理调节淀粉凝胶结构，抑制大米淀粉在储存过程中的退化

IF 2.7 2区农林科学 Q1 ENTOMOLOGY

Journal of Stored Products Research

Pub Date : 2025-12-31 DOI: 10.1016/j.jspr.2025.102946

Chen Tian , Yuxin Mu , Qianyu Hang , Xingbo Zhuang , Leiqing Pan , Cheng Zhang , Chao Ding , Qiang Liu

Starch retrogradation compromises product quality, digestibility, and shelf life, posing a challenge for the food industry in achieving stable starch-based products. Developing green and scalable inhibition strategies is therefore a key industry objective. Ultrasound-enhanced ferulic acid (FA) treatment was demonstrated to synergistically enhance rice starch gel stability. Ultrasonic cavitation modified starch matrices, increasing FA binding sites and strengthening hydrogen bonding with starch chains. Compared with non-sonicated controls, ultrasound-enhanced FA treatment reduced gel hardness by 17 % during 20d storage at 4 °C, improved water-holding capacity, and delayed amylopectin recrystallization. It also suppressed water mobility, with T₂ relaxation shortened by 50 ms, while forming stable FA-starch complexes that impeded chain reorganization. These results highlight ultrasound as a potent enhancer of FA's anti-retrogradation functionality through mechano-acoustic modification of starch molecular architecture, offering a promising pathway for developing sustainable, high-quality starch-based foods.

淀粉降解会影响产品质量、消化率和保质期，这对食品工业实现稳定的淀粉基产品提出了挑战。因此，开发绿色和可扩展的抑制策略是一个关键的行业目标。超声增强阿魏酸（FA）处理可协同提高大米淀粉凝胶的稳定性。超声空化修饰淀粉基质，增加FA结合位点，增强与淀粉链的氢键。与非超声对照相比，超声增强FA处理在4°C储存20天期间降低了17%的凝胶硬度，提高了持水能力，并延迟了支链淀粉的再结晶。它还抑制了水的流动性，使T2弛豫缩短了50 ms，同时形成了稳定的fa -淀粉配合物，阻碍了链重组。这些结果表明，超声波通过对淀粉分子结构的机械声修饰，可以有效增强FA的抗退化功能，为开发可持续的高质量淀粉基食品提供了一条有希望的途径。

{"title":"Ultrasound-enhanced ferulic acid treatment modulates starch gel structure to inhibit retrogradation in rice starch during storage","authors":"Chen Tian , Yuxin Mu , Qianyu Hang , Xingbo Zhuang , Leiqing Pan , Cheng Zhang , Chao Ding , Qiang Liu","doi":"10.1016/j.jspr.2025.102946","DOIUrl":"10.1016/j.jspr.2025.102946","url":null,"abstract":"<div><div>Starch retrogradation compromises product quality, digestibility, and shelf life, posing a challenge for the food industry in achieving stable starch-based products. Developing green and scalable inhibition strategies is therefore a key industry objective. Ultrasound-enhanced ferulic acid (FA) treatment was demonstrated to synergistically enhance rice starch gel stability. Ultrasonic cavitation modified starch matrices, increasing FA binding sites and strengthening hydrogen bonding with starch chains. Compared with non-sonicated controls, ultrasound-enhanced FA treatment reduced gel hardness by 17 % during 20d storage at 4 °C, improved water-holding capacity, and delayed amylopectin recrystallization. It also suppressed water mobility, with T<sub>2</sub> relaxation shortened by 50 ms, while forming stable FA-starch complexes that impeded chain reorganization. These results highlight ultrasound as a potent enhancer of FA's anti-retrogradation functionality through mechano-acoustic modification of starch molecular architecture, offering a promising pathway for developing sustainable, high-quality starch-based foods.</div></div>","PeriodicalId":17019,"journal":{"name":"Journal of Stored Products Research","volume":"116 ","pages":"Article 102946"},"PeriodicalIF":2.7,"publicationDate":"2025-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145880496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigations on sweet flag (Acorus calamus L.) based silver and zinc nanoparticles against Sitophilus oryzae L. in wheat 基于甜旗（Acorus calamus L.）的银锌纳米颗粒对小麦稻谷象虫的防治研究

IF 2.7 2区农林科学 Q1 ENTOMOLOGY

Journal of Stored Products Research

Pub Date : 2025-12-30 DOI: 10.1016/j.jspr.2025.102945

Kavita C. Desai , D.N. Kambrekar , V. Naveen , P.U. Krishnaraj , Suma Biradar , Rohini Sugandi , S.S. Chandrashekhar , Shivakumara K. T , Varshamrutha D. Jepi

The present study reports the green synthesis of silver nanoparticles (AgNPs) and zinc nanoparticles (ZnNPs) using Acorus calamus rhizome extract as a reducing and stabilizing agent, and their evaluation against the rice weevil, Sitophilus oryzae. The NPs were characterized through UV–visible spectrophotometry, particle size analysis, scanning electron microscopy with energy dispersive X-ray spectroscopy, X-ray diffraction, and Fourier-transform infrared spectroscopy, which confirmed their nanoscale dimensions, crystalline nature, and the presence of functional biomolecules involved in stabilization. Bioassays revealed strong insecticidal activity in a concentration-dependent manner. AgNPs at 2500 μg/mL induced 21.67 % adult mortality at 1 day after treatment (DAT) and achieved complete (100 %) mortality by 10 DAT, whereas ZnNPs at 2500 μg/mL caused 91.67 % mortality at 10 DAT and reached 100 % by 15 DAT. In comparison, ZnNPs at 500 μg/mL and A. calamus extract alone caused only 48.33 % and 36.67 % mortality, respectively, at 15 DAT, while untreated controls showed <10 % mortality. In addition to acute toxicity, AgNPs at 2500 μg/mL completely suppressed adult emergence and prevented seed damage and weight loss for up to 90 days under storage conditions. ZnNPs at 2500 μg/mL also provided significant protection, though slightly less effective. Importantly, seed germination remained unaffected across NPs treatments, indicating their safety for grain viability. Overall, green-synthesized AgNPs and ZnNPs demonstrate considerable potential as eco-friendly alternatives to synthetic insecticides for sustainable management of S. oryzae in stored grains.

本文报道了以菖蒲根提取物为还原剂和稳定剂的纳米银和纳米锌的绿色合成及其对水稻象鼻虫米象的防治效果。通过紫外可见分光光度法、粒度分析、扫描电子显微镜（x射线能谱）、x射线衍射和傅里叶变换红外光谱对NPs进行了表征，证实了它们的纳米尺度、晶体性质以及参与稳定化的功能性生物分子的存在。生物测定显示出较强的杀虫活性，且呈浓度依赖性。2500 μg/mL浓度的AgNPs在治疗后1天死亡率为21.67%，10天死亡率为100%，而2500 μg/mL浓度的ZnNPs在10天死亡率为91.67%，15天死亡率为100%。相比之下，500 μg/mL的ZnNPs和单独的菖蒲提取物在15 DAT时的死亡率分别为48.33%和36.67%，而未经处理的对照组死亡率为<； 10%。除急性毒性外，2500 μg/mL浓度的AgNPs在长达90天的储存条件下完全抑制成虫羽化，防止种子损伤和失重。2500 μg/mL的ZnNPs也具有显著的保护作用，但效果略差。重要的是，在不同的NPs处理下，种子萌发未受影响，这表明它们对谷物生存能力是安全的。总体而言，绿色合成AgNPs和ZnNPs显示出相当大的潜力，作为合成杀虫剂的生态友好替代品，可用于储粮中的稻瘟病菌的可持续管理。

{"title":"Investigations on sweet flag (Acorus calamus L.) based silver and zinc nanoparticles against Sitophilus oryzae L. in wheat","authors":"Kavita C. Desai , D.N. Kambrekar , V. Naveen , P.U. Krishnaraj , Suma Biradar , Rohini Sugandi , S.S. Chandrashekhar , Shivakumara K. T , Varshamrutha D. Jepi","doi":"10.1016/j.jspr.2025.102945","DOIUrl":"10.1016/j.jspr.2025.102945","url":null,"abstract":"<div><div>The present study reports the green synthesis of silver nanoparticles (AgNPs) and zinc nanoparticles (ZnNPs) using <em>Acorus calamus</em> rhizome extract as a reducing and stabilizing agent, and their evaluation against the rice weevil, <em>Sitophilus oryzae</em>. The NPs were characterized through UV–visible spectrophotometry, particle size analysis, scanning electron microscopy with energy dispersive X-ray spectroscopy, X-ray diffraction, and Fourier-transform infrared spectroscopy, which confirmed their nanoscale dimensions, crystalline nature, and the presence of functional biomolecules involved in stabilization. Bioassays revealed strong insecticidal activity in a concentration-dependent manner. AgNPs at 2500 μg/mL induced 21.67 % adult mortality at 1 day after treatment (DAT) and achieved complete (100 %) mortality by 10 DAT, whereas ZnNPs at 2500 μg/mL caused 91.67 % mortality at 10 DAT and reached 100 % by 15 DAT. In comparison, ZnNPs at 500 μg/mL and <em>A. calamus</em> extract alone caused only 48.33 % and 36.67 % mortality, respectively, at 15 DAT, while untreated controls showed <10 % mortality. In addition to acute toxicity, AgNPs at 2500 μg/mL completely suppressed adult emergence and prevented seed damage and weight loss for up to 90 days under storage conditions. ZnNPs at 2500 μg/mL also provided significant protection, though slightly less effective. Importantly, seed germination remained unaffected across NPs treatments, indicating their safety for grain viability. Overall, green-synthesized AgNPs and ZnNPs demonstrate considerable potential as eco-friendly alternatives to synthetic insecticides for sustainable management of <em>S. oryzae</em> in stored grains.</div></div>","PeriodicalId":17019,"journal":{"name":"Journal of Stored Products Research","volume":"116 ","pages":"Article 102945"},"PeriodicalIF":2.7,"publicationDate":"2025-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145880382","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Deep optimizer-informed empirical twin for cold plasma jet-assisted carrot drying: Towards a smarter alternative to black-box AI-based models 深度优化告知经验双胞胎冷等离子射流辅助胡萝卜干燥：朝着更智能的替代黑箱人工智能模型

IF 2.7 2区农林科学 Q1 ENTOMOLOGY

Journal of Stored Products Research

Pub Date : 2025-12-30 DOI: 10.1016/j.jspr.2025.102939

Bobby Shekarau Luka , Satyananda Kar , Suryasunil Rath , Mohammed Haris Siddiqui , Khwaja Osama

This study presents an innovative framework that integrates Hybrid Machine Learning (HML) and Deep Optimizer-Informed Empirical Modelling (DOIEM) to predict moisture dynamics during cold plasma jet-assisted drying of carrot slices. Carrot samples were bifacially treated with cold plasma for varying durations of 30, 60 and 90 s and dried using vacuum and convective tray dryers. Moisture ratio data were modelled using Decision Tree, Support Vector Regression, Gaussian Process Regression, Deep Feed-Forward Neural Network, and their hybrid. The HML framework demonstrated strong predictive capability across drying methods and pre-treatment conditions, maintaining consistent performance when tested on unseen data. The DOIEM approach further enhanced traditional empirical models by using a deep-learning optimizer (Adam) to improve parameter estimation and generate a two-dimensional moisture map for an offline digital twin. The resulting digital twin tracked and updated model parameters to reflect spatiotemporal changes in moisture, offering excellent prediction and interpretability. Comparatively, DOIEM provided superior modelling performance compared to the hybrid ML model in both vacuum and tray drying. The DOIEM-based twin also showed strong potential for integration into sensor-driven smart drying systems. This work establishes a practical and data-driven foundation for intelligent food drying applications and demonstrates how digital twin technologies can support real-time monitoring and control in agri-food processing.

本研究提出了一个创新的框架，该框架集成了混合机器学习（HML）和深度优化告知经验模型（DOIEM），以预测冷等离子体射流辅助干燥胡萝卜片过程中的水分动态。胡萝卜样品用冷等离子体双面处理30、60和90秒，然后用真空和对流托盘干燥器干燥。采用决策树、支持向量回归、高斯过程回归、深度前馈神经网络及其混合模型对含水率数据进行建模。HML框架在干燥方法和预处理条件下表现出强大的预测能力，在对未知数据进行测试时保持一致的性能。DOIEM方法通过使用深度学习优化器（Adam）来改进参数估计，并为离线数字孪生生成二维湿度图，从而进一步增强了传统的经验模型。由此产生的数字孪生跟踪和更新了模型参数，以反映湿度的时空变化，提供了极好的预测和可解释性。相比之下，DOIEM在真空和托盘干燥中都提供了优于混合ML模型的建模性能。基于doiem的孪生体也显示出集成到传感器驱动的智能干燥系统中的强大潜力。这项工作为智能食品干燥应用奠定了实用和数据驱动的基础，并展示了数字孪生技术如何支持农业食品加工中的实时监控。

{"title":"Deep optimizer-informed empirical twin for cold plasma jet-assisted carrot drying: Towards a smarter alternative to black-box AI-based models","authors":"Bobby Shekarau Luka , Satyananda Kar , Suryasunil Rath , Mohammed Haris Siddiqui , Khwaja Osama","doi":"10.1016/j.jspr.2025.102939","DOIUrl":"10.1016/j.jspr.2025.102939","url":null,"abstract":"<div><div>This study presents an innovative framework that integrates Hybrid Machine Learning (HML) and Deep Optimizer-Informed Empirical Modelling (DOIEM) to predict moisture dynamics during cold plasma jet-assisted drying of carrot slices. Carrot samples were bifacially treated with cold plasma for varying durations of 30, 60 and 90 s and dried using vacuum and convective tray dryers. Moisture ratio data were modelled using Decision Tree, Support Vector Regression, Gaussian Process Regression, Deep Feed-Forward Neural Network, and their hybrid. The HML framework demonstrated strong predictive capability across drying methods and pre-treatment conditions, maintaining consistent performance when tested on unseen data. The DOIEM approach further enhanced traditional empirical models by using a deep-learning optimizer (Adam) to improve parameter estimation and generate a two-dimensional moisture map for an offline digital twin. The resulting digital twin tracked and updated model parameters to reflect spatiotemporal changes in moisture, offering excellent prediction and interpretability. Comparatively, DOIEM provided superior modelling performance compared to the hybrid ML model in both vacuum and tray drying. The DOIEM-based twin also showed strong potential for integration into sensor-driven smart drying systems. This work establishes a practical and data-driven foundation for intelligent food drying applications and demonstrates how digital twin technologies can support real-time monitoring and control in agri-food processing.</div></div>","PeriodicalId":17019,"journal":{"name":"Journal of Stored Products Research","volume":"116 ","pages":"Article 102939"},"PeriodicalIF":2.7,"publicationDate":"2025-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145880498","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Experimental study, performance analysis, and optimization of hot Air–Membrane coupled dehumidification for ultra-drying of pumpkin seeds 南瓜籽超干燥热空气-膜耦合除湿的实验研究、性能分析及优化

IF 2.7 2区农林科学 Q1 ENTOMOLOGY

Journal of Stored Products Research

Pub Date : 2025-12-30 DOI: 10.1016/j.jspr.2025.102930

Yan Wang , Jiaoling Wang , Yongchang Li , Tianhang Ding , Feihu Song , Guangyuan Jin , Xianfeng Li , Chunfang Song , Weidong Song

Ultra-dry storage (less than 5 % moisture content, d.b.) can prolong the storage life of seeds. A hot air-membrane coupled dehumidification drying (HAMDH) system was developed for ultra-drying pumpkin seeds. Pumpkin seeds were dried at different temperatures (40 °C, 50 °C, and 60 °C) and ambient relative humidities (3 %, 7 %, and 11 %) to 3.5 % (d.b.). Drying kinetics, mathematical modeling, energy consumption, and seed quality, including germination characteristics, membrane lipid peroxidation, and antioxidant enzyme activities, were systematically investigated. The results showed that the Page model accurately described the drying behavior (R² > 0.998), with effective moisture diffusivity (D_eff) between 3.31 × 10⁻¹⁰ and 9.56 × 10⁻¹⁰ m²/s. Drying time decreased significantly with increasing temperature, while specific energy consumption (SEC) was influenced by both temperature and relative humidity. At 50 °C, pumpkin seeds still maintained good germination characteristics, with the lowest malondialdehyde (MDA) content and higher antioxidant enzyme activity compared to those at 40 °C. Response surface methodology (RSM) for multi-objective optimization was used to optimize the process parameters, identifying 51.5 °C and 7.5 % humidity as optimal. The predicted optimal values were as follows: drying time of 225.56 min, D_eff of 5.80 × 10⁻¹⁰ m²/s, Germination percentage of 95.56 %, MDA content of 3.32 nmol/g, and catalase activity of 268.22 U/(g·min), respectively. This study demonstrates that the HAMDH process could extend the storage lifespan of seeds while maintaining their bioactivity in oilseed crops.

超干贮藏（水分含量低于5%，d.b）可以延长种子的贮藏寿命。研制了一种用于南瓜籽超干燥的热空气-膜耦合除湿干燥系统。南瓜籽在不同温度（40°C、50°C和60°C）和环境相对湿度（3%、7%和11%）至3.5% （d.b.b）下干燥。系统地研究了干燥动力学、数学模型、能量消耗和种子质量，包括发芽特性、膜脂过氧化和抗氧化酶活性。结果表明，Page模型能较好地描述干燥行为（R2 > 0.998），有效水分扩散系数（Deff）在3.31 × 10−10 ~ 9.56 × 10−10 m2/s之间。干燥时间随温度的升高而显著缩短，而比能耗（SEC）受温度和相对湿度的共同影响。与40℃处理相比，50℃处理的南瓜籽仍保持良好的萌发特性，丙二醛（MDA）含量最低，抗氧化酶活性较高。采用多目标优化响应面法（RSM）对工艺参数进行优化，确定51.5℃、7.5%湿度为最佳工艺参数。预测的最优值为：干燥时间225.56 min, Deff 5.80 × 10−10 m2/s，发芽率95.56%，MDA含量3.32 nmol/g，过氧化氢酶活性268.22 U/（g·min）。本研究表明，HAMDH处理可以延长油籽作物种子的贮藏寿命，同时保持其生物活性。

{"title":"Experimental study, performance analysis, and optimization of hot Air–Membrane coupled dehumidification for ultra-drying of pumpkin seeds","authors":"Yan Wang , Jiaoling Wang , Yongchang Li , Tianhang Ding , Feihu Song , Guangyuan Jin , Xianfeng Li , Chunfang Song , Weidong Song","doi":"10.1016/j.jspr.2025.102930","DOIUrl":"10.1016/j.jspr.2025.102930","url":null,"abstract":"<div><div>Ultra-dry storage (less than 5 % moisture content, <em>d.b.</em>) can prolong the storage life of seeds. A hot air-membrane coupled dehumidification drying (HAMDH) system was developed for ultra-drying pumpkin seeds. Pumpkin seeds were dried at different temperatures (40 °C, 50 °C, and 60 °C) and ambient relative humidities (3 %, 7 %, and 11 %) to 3.5 % (d.b.). Drying kinetics, mathematical modeling, energy consumption, and seed quality, including germination characteristics, membrane lipid peroxidation, and antioxidant enzyme activities, were systematically investigated. The results showed that the Page model accurately described the drying behavior (<em>R</em><sup>2</sup> > 0.998), with effective moisture diffusivity (<em>D</em><sub><em>eff</em></sub>) between 3.31 × 10<sup>−10</sup> and 9.56 × 10<sup>−10</sup> m<sup>2</sup>/s. Drying time decreased significantly with increasing temperature, while specific energy consumption (<em>SEC</em>) was influenced by both temperature and relative humidity. At 50 °C, pumpkin seeds still maintained good germination characteristics, with the lowest malondialdehyde (MDA) content and higher antioxidant enzyme activity compared to those at 40 °C. Response surface methodology (RSM) for multi-objective optimization was used to optimize the process parameters, identifying 51.5 °C and 7.5 % humidity as optimal. The predicted optimal values were as follows: drying time of 225.56 min, <em>D</em><sub><em>eff</em></sub> <em>of</em> 5.80 × 10<sup>−10</sup> m<sup>2</sup>/s, Germination percentage of 95.56 %, MDA content of 3.32 nmol/g, and catalase activity of 268.22 U/(g·min), respectively. This study demonstrates that the HAMDH process could extend the storage lifespan of seeds while maintaining their bioactivity in oilseed crops.</div></div>","PeriodicalId":17019,"journal":{"name":"Journal of Stored Products Research","volume":"116 ","pages":"Article 102930"},"PeriodicalIF":2.7,"publicationDate":"2025-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145880501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0