Dietary supplements are concentrated sources of vitamins, minerals, or other substances with nutritional or physiological effects intended to supplement the regular diet. The aim of this study was the chemical characterization of methanolic extracts from two edible mushrooms, Cantharellus cibarius Fr. (1821) (Cantharellaceae) and Lactarius piperatus (L.) Roussel (1806) (Russulaceae), and the investigation of their potential use as dietary supplements in the rearing of Plodia interpunctella (Hübner, 1813) (Lepidoptera: Pyralidae) laboratory populations. This was examined by assessing the effect of the extracts on survival rate and life history parameters of P. interpunctella: mean developmental duration (MDD), adult emergence dynamics, longevity and fecundity on maize kernels. The attractant potential of the mushroom extracts was also investigated, by examining their influence on the oviposition preference of mated females. The highest number of emerged adults was recorded with 1 % L. piperatus extract (46.5 ± 5.27). Significantly shorter MDD was observed with 5 % C. cibarius (32.48 ± 0.53) and 5 % L. piperatus (32.73 ± 0.41) extract treatments, compared to the control. Adult emergence lasted significantly longer in the treatments (15.75 ± 0.25), compared to the control. The highest fecundity was observed with 5 % L. piperatus extract treatment (163.38 ± 10.21). The oviposition preference test confirmed that treatments with 2 % C. cibarius extracts are attractant, while L. piperatus extracts have no attractant potential. Chemical analysis of both extracts was performed, including qualitative and quantitative analysis of polyphenols, analysis of mineral elements, and determination of anions of organic acids, sugars and sugar alcohols, confirming a high nutrient content.
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