Journal of Stored Products Research最新文献_第7页

Morphological and molecular characterization of major internal feeders in storage insect pests using SWISS- model

IF 2.7 2区农林科学 Q1 ENTOMOLOGY

Journal of Stored Products Research

Pub Date : 2024-12-22 DOI: 10.1016/j.jspr.2024.102523

B. Keerthana , G. Preetha , V.R. Saminathan , M. Murugan , T. Eevera , D. Ramesh

The present investigation focuses on morphological, morphometrics and molecular characterization of four significant insect pests of stored grains, Callosobruchus maculatus (F.), Callosobruchus chinensis (L.), Caryedon serratus (Olivier) and Sitophilus oryzae (L.). Detailed morphological descriptions of egg, larval, pupal and adult stages, including color patterns, anatomical features and genital structures were provided for each species. Morphometric measurements of various life stages of each pest was recorded. Molecular characterization of insects involved extracting genomic DNA and utilizing mitochondrial cytochrome oxidase I (mt COI) as a molecular marker to identify and differentiate the species. Sequencing and phylogenetic analysis confirmed species identities, revealing high genetic similarities with previously documented populations. The sequences were submitted to GenBank and an accession number was generated for Callosobruchus maculatus, Callosobruchus chinensis, Caryedon serratus, and Sitophilus oryzae. Additionally, protein modeling and structure validation were conducted using SWISS-MODEL with Ramachandran plots confirming the high quality of the predicted protein structures. This study aids in accurately identifying and understanding these pests morphology, morphometrics and molecular characterization and contributes to more effective pest management strategies.

{"title":"Morphological and molecular characterization of major internal feeders in storage insect pests using SWISS- model","authors":"B. Keerthana , G. Preetha , V.R. Saminathan , M. Murugan , T. Eevera , D. Ramesh","doi":"10.1016/j.jspr.2024.102523","DOIUrl":"10.1016/j.jspr.2024.102523","url":null,"abstract":"<div><div>The present investigation focuses on morphological, morphometrics and molecular characterization of four significant insect pests of stored grains, <em>Callosobruchus maculatus</em> (F.), <em>Callosobruchus chinensis</em> (L.), <em>Caryedon serratus</em> (Olivier) and <em>Sitophilus oryzae</em> (L.). Detailed morphological descriptions of egg, larval, pupal and adult stages, including color patterns, anatomical features and genital structures were provided for each species. Morphometric measurements of various life stages of each pest was recorded. Molecular characterization of insects involved extracting genomic DNA and utilizing mitochondrial cytochrome oxidase I (mt COI) as a molecular marker to identify and differentiate the species. Sequencing and phylogenetic analysis confirmed species identities, revealing high genetic similarities with previously documented populations. The sequences were submitted to GenBank and an accession number was generated for <em>Callosobruchus maculatus, Callosobruchus chinensis, Caryedon serratus,</em> and <em>Sitophilus oryzae</em>. Additionally, protein modeling and structure validation were conducted using SWISS-MODEL with Ramachandran plots confirming the high quality of the predicted protein structures. This study aids in accurately identifying and understanding these pests morphology, morphometrics and molecular characterization and contributes to more effective pest management strategies.</div></div>","PeriodicalId":17019,"journal":{"name":"Journal of Stored Products Research","volume":"111 ","pages":"Article 102523"},"PeriodicalIF":2.7,"publicationDate":"2024-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143104471","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Changes in intergranular air properties and fatty acid profile of wheat bulk in a large warehouse with air-conditioned oversummering

IF 2.7 2区农林科学 Q1 ENTOMOLOGY

Journal of Stored Products Research

Pub Date : 2024-12-20 DOI: 10.1016/j.jspr.2024.102520

Mengya Wang , Minghui Zhao , Xuhui Zhuang , Jianzhang Wu , Xingjun Li , Linlin Song , Yushan Jiang

Effect of air-condition on wheat's oversummering in a large flat warehouse urgently need to study. In this study, the air-condition was set at 25 °C in the second and third summer of wheat storage in No.19 warehouse of Pingyuan Grain Storage Depot, Shandong province, China. The intergranular air properties of grain bulk were calculated by our developed software. Compared with the control warehouse (No.17), the surface, second, third, and fourth layers of wheat bulk in No.19 warehouse during the running of air-conditioners decreased by 3.4 °C, 2 °C, 1.5 °C and 1.35 °C, respectively, the humidity ratio, relative humidity and wet-bulb temperature in the surface layer and whole bulk respectively decreased by 3.85 and 1.5 g/kg, 3.55% and 3.85%, 3.65 °C and 2.2 °C. Air-conditioning significantly decreased the bulk surface temperature. Free fatty acid (FFA) values in each layer of grain bulk increased continuously since warehousing, and the average FFA value in No.17 warehouse managed by conventional technology was below 40 mg KOH/100g. Twenty-seven species of fatty acids were identified in wheat. Contents of the total and unsaturated fatty acids increased respectively from 11703 to 9348 μg/g at 124-day storage to 14758 and 11834 μg/g at 841-day storage for wheat bulk in No.17 warehouse, but increased respectively from 11674 to 9321 μg/g to 16235 and 13027 μg/g for grain bulk in No.19 warehouse. Infrared spectroscopy confirmed that during 841-day storage, the absorption values of C-H stretching vibration (2919.7 cm⁻¹) of saturated carbon and C-H stretching vibration (3304.9 cm⁻¹) of unsaturated carbon in No.19 warehouse were significantly higher than those of No.17 warehouse. Scanning electron microscopy shows that air-conditioned warehouse effectively maintain the internal distinct microstructure of wheat grains. These results suggest that air-conditioning can reduce the bulk surface temperature and FAA value of stored wheat and keep the content of unsaturated fatty acids, and FFA value of properly stored wheat can be assumed as ≤50 mg KOH/100g.

{"title":"Changes in intergranular air properties and fatty acid profile of wheat bulk in a large warehouse with air-conditioned oversummering","authors":"Mengya Wang , Minghui Zhao , Xuhui Zhuang , Jianzhang Wu , Xingjun Li , Linlin Song , Yushan Jiang","doi":"10.1016/j.jspr.2024.102520","DOIUrl":"10.1016/j.jspr.2024.102520","url":null,"abstract":"<div><div>Effect of air-condition on wheat's oversummering in a large flat warehouse urgently need to study. In this study, the air-condition was set at 25 °C in the second and third summer of wheat storage in No.19 warehouse of Pingyuan Grain Storage Depot, Shandong province, China. The intergranular air properties of grain bulk were calculated by our developed software. Compared with the control warehouse (No.17), the surface, second, third, and fourth layers of wheat bulk in No.19 warehouse during the running of air-conditioners decreased by 3.4 °C, 2 °C, 1.5 °C and 1.35 °C, respectively, the humidity ratio, relative humidity and wet-bulb temperature in the surface layer and whole bulk respectively decreased by 3.85 and 1.5 g/kg, 3.55% and 3.85%, 3.65 °C and 2.2 °C. Air-conditioning significantly decreased the bulk surface temperature. Free fatty acid (FFA) values in each layer of grain bulk increased continuously since warehousing, and the average FFA value in No.17 warehouse managed by conventional technology was below 40 mg KOH/100g. Twenty-seven species of fatty acids were identified in wheat. Contents of the total and unsaturated fatty acids increased respectively from 11703 to 9348 μg/g at 124-day storage to 14758 and 11834 μg/g at 841-day storage for wheat bulk in No.17 warehouse, but increased respectively from 11674 to 9321 μg/g to 16235 and 13027 μg/g for grain bulk in No.19 warehouse. Infrared spectroscopy confirmed that during 841-day storage, the absorption values of C-H stretching vibration (2919.7 cm<sup>−1</sup>) of saturated carbon and C-H stretching vibration (3304.9 cm<sup>−1</sup>) of unsaturated carbon in No.19 warehouse were significantly higher than those of No.17 warehouse. Scanning electron microscopy shows that air-conditioned warehouse effectively maintain the internal distinct microstructure of wheat grains. These results suggest that air-conditioning can reduce the bulk surface temperature and FAA value of stored wheat and keep the content of unsaturated fatty acids, and FFA value of properly stored wheat can be assumed as ≤50 mg KOH/100g.</div></div>","PeriodicalId":17019,"journal":{"name":"Journal of Stored Products Research","volume":"111 ","pages":"Article 102520"},"PeriodicalIF":2.7,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143104470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Application of reuterin-containing edible coating to inhibit the growth of aflatoxigenic Aspergillus flavus on nutmeg

IF 2.7 2区农林科学 Q1 ENTOMOLOGY

Journal of Stored Products Research

Pub Date : 2024-12-18 DOI: 10.1016/j.jspr.2024.102519

Widiati Purnawita , Winiati Pudji Rahayu , Hanifah Nuryani Lioe , Siti Nurjanah

Contamination of Aspergillus flavus (A. flavus) and aflatoxin in nutmeg remains problematic due to the poor postharvest handling practices and the climate, which is favorable for fungal growth in Indonesia. Using the natural antifungal agents can be an alternative measure to control the aflatoxin-producing fungal growth. This current study aimed to evaluate the efficacy of reuterin in inhibiting aflatoxigenic A. flavus growth on the nutmeg during storage. Reuterin at concentrations of 4, 6, 8, and 10 × MIC (1 MIC = 6 mM) was incorporated into a carboxymethyl cellulose (CMC)-based edible coating to coat the surface of nutmeg spiked with A. flavus conidia (2.5 × 10² CFU/g). Results showed that reuterin effectively extended the lag phase of A. flavus growth on the coated nutmeg and disrupted the hyphal development and conidiation. At a minimum concentration of 48 mM (equal to 3.63 mg/mL coating solution or 630 mg/kg nutmeg), reuterin inhibited completely the growth of A. flavus on the nutmeg stored at an RH of 97% and a temperature of 30 °C for 30 days. This study confirmed that the application of reuterin incorporated into a CMC-based edible coating was an effective measure for inhibiting aflatoxigenic A. flavus growth on the nutmeg during storage.

{"title":"Application of reuterin-containing edible coating to inhibit the growth of aflatoxigenic Aspergillus flavus on nutmeg","authors":"Widiati Purnawita , Winiati Pudji Rahayu , Hanifah Nuryani Lioe , Siti Nurjanah","doi":"10.1016/j.jspr.2024.102519","DOIUrl":"10.1016/j.jspr.2024.102519","url":null,"abstract":"<div><div>Contamination of <em>Aspergillus flavus</em> (<em>A. flavus</em>) and aflatoxin in nutmeg remains problematic due to the poor postharvest handling practices and the climate, which is favorable for fungal growth in Indonesia. Using the natural antifungal agents can be an alternative measure to control the aflatoxin-producing fungal growth. This current study aimed to evaluate the efficacy of reuterin in inhibiting aflatoxigenic <em>A. flavus</em> growth on the nutmeg during storage. Reuterin at concentrations of 4, 6, 8, and 10 × MIC (1 MIC = 6 mM) was incorporated into a carboxymethyl cellulose (CMC)-based edible coating to coat the surface of nutmeg spiked with <em>A. flavus</em> conidia (2.5 × 10<sup>2</sup> CFU/g). Results showed that reuterin effectively extended the lag phase of <em>A. flavus</em> growth on the coated nutmeg and disrupted the hyphal development and conidiation. At a minimum concentration of 48 mM (equal to 3.63 mg/mL coating solution or 630 mg/kg nutmeg), reuterin inhibited completely the growth of <em>A. flavus</em> on the nutmeg stored at an RH of 97% and a temperature of 30 °C for 30 days. This study confirmed that the application of reuterin incorporated into a CMC-based edible coating was an effective measure for inhibiting aflatoxigenic <em>A. flavus</em> growth on the nutmeg during storage.</div></div>","PeriodicalId":17019,"journal":{"name":"Journal of Stored Products Research","volume":"111 ","pages":"Article 102519"},"PeriodicalIF":2.7,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143104469","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sublethal impact of pirimiphos-methyl and etofenprox on mandible and hindwing morphology in Alphitobius diaperinus

IF 2.7 2区农林科学 Q1 ENTOMOLOGY

Journal of Stored Products Research

Pub Date : 2024-12-16 DOI: 10.1016/j.jspr.2024.102482

Demeter Lorentha S. Gidari , Vladimir Žikić , Maria C. Boukouvala , Nickolas G. Kavallieratos , Maja Lazarević

Insecticidal applications can cause morphological deformities to the exposed insects or their offspring. Pirimiphos-methyl and etofenprox have been previously evaluated against Alphitobius diaperinus, but there is no knowledge about their effect on the morphology of the F1 generation. This study examines the effect of both insecticides on mandible and hindwing morphology of A. diaperinus adult progeny. Regarding the shape of the mandibles in both female and male individuals, the two insecticides induced significant deformations compared to the control group. Etofenprox caused more vigorous morphological changes vs. pirimiphos-methyl. In terms of hindwings, Principal Component Analysis (PCA) did not reveal distinction between females or males exposed to either etofenprox or pirimiphos-methyl. Both insecticides affected the mouthparts of the F1 generation adults of A. diaperinus, which could lead to reduced feeding ability and thus to insufficient population growth.

{"title":"Sublethal impact of pirimiphos-methyl and etofenprox on mandible and hindwing morphology in Alphitobius diaperinus","authors":"Demeter Lorentha S. Gidari , Vladimir Žikić , Maria C. Boukouvala , Nickolas G. Kavallieratos , Maja Lazarević","doi":"10.1016/j.jspr.2024.102482","DOIUrl":"10.1016/j.jspr.2024.102482","url":null,"abstract":"<div><div>Insecticidal applications can cause morphological deformities to the exposed insects or their offspring. Pirimiphos-methyl and etofenprox have been previously evaluated against <em>Alphitobius diaperinus,</em> but there is no knowledge about their effect on the morphology of the F1 generation. This study examines the effect of both insecticides on mandible and hindwing morphology of <em>A. diaperinus</em> adult progeny. Regarding the shape of the mandibles in both female and male individuals, the two insecticides induced significant deformations compared to the control group. Etofenprox caused more vigorous morphological changes vs. pirimiphos-methyl. In terms of hindwings, Principal Component Analysis (PCA) did not reveal distinction between females or males exposed to either etofenprox or pirimiphos-methyl. Both insecticides affected the mouthparts of the F1 generation adults of <em>A. diaperinus</em>, which could lead to reduced feeding ability and thus to insufficient population growth.</div></div>","PeriodicalId":17019,"journal":{"name":"Journal of Stored Products Research","volume":"111 ","pages":"Article 102482"},"PeriodicalIF":2.7,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143180505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bioactive composition, microstructure, and physicochemical properties of Arbutus unedo berries dried using different techniques

IF 2.7 2区农林科学 Q1 ENTOMOLOGY

Journal of Stored Products Research

Pub Date : 2024-12-14 DOI: 10.1016/j.jspr.2024.102501

Ibtissem Refas , Malek Amiali , Oluwafemi Ayodele George , Kieu Hiep Le , Narjes Malekjani , Abdolreza Kharaghani

This research examined the impact of infrared (IR) and convective drying (CV) at varying temperatures (40, 60, and 80 °C) and freeze-drying (FD) on the microstructure, drying kinetics, and quality attributes of strawberry tree fruit (Arbutus unedo L.), including phenolic content (TPC), flavonoids (TFC), anthocyanins (TA), antioxidant activity, color, rehydration ratio, shrinkage, and bulk density. Results showed that elevated temperatures accelerated the drying process, with IR at 80 °C reducing the duration by 69% compared to CV. IR drying was particularly effective in preserving the fruit's red color, achieving the lowest ΔE values (8 ± 4.59) and showing minimal color change at 80 °C. While drying generally increased TFC and TPC, the impact on antioxidant activity was negligible, except in IR drying at 60 °C. However, TA was largely reduced, mainly due to prolonged processing times. SEM analysis highlighted that FD samples maintained a more open structure, while IR samples showed desirable microstructure preservation, leading to improved moisture retention. The Midilli model best described the drying process of the strawberry tree berries, with diffusivity ranging from 0.96 to 7.07 ( × 10⁻¹⁰ m²/s) for CV and 0.42 to 2.57 ( × 10⁻⁹ m²/s) for IR. Activation energies were calculated as 45.72 kJ/mol for CV and 41.91 kJ/mol for IR. Overall, the study suggests that IR at 80 °C is the most efficient method for drying Arbutus unedo, based on its effectiveness in drying time reduction, preservation of color, anthocyanins, microstructural integrity, and minimizing shrinkage, while maximizing the rehydration ratio compared to CV.

{"title":"Bioactive composition, microstructure, and physicochemical properties of Arbutus unedo berries dried using different techniques","authors":"Ibtissem Refas , Malek Amiali , Oluwafemi Ayodele George , Kieu Hiep Le , Narjes Malekjani , Abdolreza Kharaghani","doi":"10.1016/j.jspr.2024.102501","DOIUrl":"10.1016/j.jspr.2024.102501","url":null,"abstract":"<div><div>This research examined the impact of infrared (IR) and convective drying (CV) at varying temperatures (40, 60, and 80 °C) and freeze-drying (FD) on the microstructure, drying kinetics, and quality attributes of strawberry tree fruit (<em>Arbutus unedo</em> L.), including phenolic content (TPC), flavonoids (TFC), anthocyanins (TA), antioxidant activity, color, rehydration ratio, shrinkage, and bulk density. Results showed that elevated temperatures accelerated the drying process, with IR at 80 °C reducing the duration by 69% compared to CV. IR drying was particularly effective in preserving the fruit's red color, achieving the lowest ΔE values (8 ± 4.59) and showing minimal color change at 80 °C. While drying generally increased TFC and TPC, the impact on antioxidant activity was negligible, except in IR drying at 60 °C. However, TA was largely reduced, mainly due to prolonged processing times. SEM analysis highlighted that FD samples maintained a more open structure, while IR samples showed desirable microstructure preservation, leading to improved moisture retention. The Midilli model best described the drying process of the strawberry tree berries, with diffusivity ranging from 0.96 to 7.07 ( × 10<sup>−10</sup> m<sup>2</sup>/s) for CV and 0.42 to 2.57 ( × 10<sup>−9</sup> m<sup>2</sup>/s) for IR. Activation energies were calculated as 45.72 kJ/mol for CV and 41.91 kJ/mol for IR. Overall, the study suggests that IR at 80 °C is the most efficient method for drying <em>Arbutus unedo</em>, based on its effectiveness in drying time reduction, preservation of color, anthocyanins, microstructural integrity, and minimizing shrinkage, while maximizing the rehydration ratio compared to CV.</div></div>","PeriodicalId":17019,"journal":{"name":"Journal of Stored Products Research","volume":"111 ","pages":"Article 102501"},"PeriodicalIF":2.7,"publicationDate":"2024-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143104467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chemical compositions and insecticidal activities of Tetradium ruticarpum essential oils against Lasioderma serricorne

IF 2.7 2区农林科学 Q1 ENTOMOLOGY

Journal of Stored Products Research

Pub Date : 2024-12-14 DOI: 10.1016/j.jspr.2024.102517

Yu-Chen Zhang , Ao Liu , Jia-Zhu Wang , Yuan-Tong Qi , Shu-Shan Du

Stored products are susceptible to insect infestation. Many plant essential oils (EOs) have been used to control various pests, and compared to conventional pesticides, these natural products generally pose lower risks to humans and the environment. This study aimed to evaluate the insecticidal and repellent properties of EOs extracted from Tetradium ruticarpum (formerly Euodia rutaecarpa) fruits at different collection times against Lasioderma serricorne. EOs were obtained through hydrodistillation and analyzed using gas chromatography-mass spectrometry (GC-MS). The oil yield was 1.18 v/w. A total of 41 compounds were identified, with monoterpenes dominating in most samples. Except for the TRF-7 sample, where the main compound was 1,3-dicyclohexylpropane (45.9%), the predominant compound in all other EOs was 3-carene, with contents exceeding 87%. According to Principal Component Analysis (PCA), the composition of TRF-7 EO differed significantly from the other EOs due to the different collection times. The bio-assays showed that EOs demonstrated strong contact toxicity and repellent effects against L. serricorne, but fumigant toxicity was relatively weak. Among the tested samples, TRF-2 and TRF-4 exhibited the highest contact toxicity, with LD₅₀ values of 2.76 mg/L air and 4.08 mg/L air, respectively. Repellency was effective at higher concentrations but diminished with lower doses and extended exposure. These results suggest that EOs from T. ruticarpum fruits hold potential as botanical insecticides for protecting stored products.

{"title":"Chemical compositions and insecticidal activities of Tetradium ruticarpum essential oils against Lasioderma serricorne","authors":"Yu-Chen Zhang , Ao Liu , Jia-Zhu Wang , Yuan-Tong Qi , Shu-Shan Du","doi":"10.1016/j.jspr.2024.102517","DOIUrl":"10.1016/j.jspr.2024.102517","url":null,"abstract":"<div><div>Stored products are susceptible to insect infestation. Many plant essential oils (EOs) have been used to control various pests, and compared to conventional pesticides, these natural products generally pose lower risks to humans and the environment. This study aimed to evaluate the insecticidal and repellent properties of EOs extracted from <em>Tetradium ruticarpum</em> (formerly <em>Euodia rutaecarpa</em>) fruits at different collection times against <em>Lasioderma serricorne</em>. EOs were obtained through hydrodistillation and analyzed using gas chromatography-mass spectrometry (GC-MS). The oil yield was 1.18 v/w. A total of 41 compounds were identified, with monoterpenes dominating in most samples. Except for the TRF-7 sample, where the main compound was 1,3-dicyclohexylpropane (45.9%), the predominant compound in all other EOs was 3-carene, with contents exceeding 87%. According to Principal Component Analysis (PCA), the composition of TRF-7 EO differed significantly from the other EOs due to the different collection times. The bio-assays showed that EOs demonstrated strong contact toxicity and repellent effects against <em>L. serricorne</em>, but fumigant toxicity was relatively weak. Among the tested samples, TRF-2 and TRF-4 exhibited the highest contact toxicity, with LD<sub>50</sub> values of 2.76 mg/L air and 4.08 mg/L air, respectively. Repellency was effective at higher concentrations but diminished with lower doses and extended exposure. These results suggest that EOs from <em>T. ruticarpum</em> fruits hold potential as botanical insecticides for protecting stored products.</div></div>","PeriodicalId":17019,"journal":{"name":"Journal of Stored Products Research","volume":"111 ","pages":"Article 102517"},"PeriodicalIF":2.7,"publicationDate":"2024-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143104468","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Carrageenan based edible coating modulates oxidative stress and delays cell wall polysaccharides disassembly in harvested grapefruit

IF 2.7 2区农林科学 Q1 ENTOMOLOGY

Journal of Stored Products Research

Pub Date : 2024-12-13 DOI: 10.1016/j.jspr.2024.102514

Sajid Ali , Sidra Riaz , Hafiz Umer Javed , Ahmad Sattar Khan , Maratab Ali , Safina Naz , Hasan Sardar , Muhammad Shahzad Saleem

The cell wall degradation results in firmness loss thus negatively affecting the overall eating quality. So, in this work, the influence of carrageenan [0, 0.5, 1.0 and 1.5% (w/v)] coating was studied on ‘Shamber’ grapefruit during storage at 20 ± 1 °C for 42 days (d). The 1.5% carrageenan concentration suppressed metabolic activities and reduced disease rate (about 41%), weight loss (around 46%), malondialdehyde, electrolytes leakage, superoxide anion and hydrogen peroxide. The higher antioxidant enzyme activity as well as total flavonoids, ascorbic acid and total phenolic contents were noted in 1.5% treatment. The 1.5% coating delayed the increase in water-soluble pectin (WSP) and suppressed the reduction of sodium carbonate soluble pectin (NCSP), protopectin, chelate soluble pectin (CSP), hemicellulose and cellulose content. The grapefruit coated with 1.5% carrageenan had lower activities of cellulase (CEL), polygalacturonase (PG), β-galactosidase (β-gal) and pectin methylesterase (PME) enzyme along with higher firmness. The 1.5% carrageenan preserved higher titratable acidity and reduced increase in total soluble solids and ripening index in coated grapefruit. In conclusion, 1.5% carrageenan coating could be considered appropriate for delaying the quality deterioration in grapefruit.

{"title":"Carrageenan based edible coating modulates oxidative stress and delays cell wall polysaccharides disassembly in harvested grapefruit","authors":"Sajid Ali , Sidra Riaz , Hafiz Umer Javed , Ahmad Sattar Khan , Maratab Ali , Safina Naz , Hasan Sardar , Muhammad Shahzad Saleem","doi":"10.1016/j.jspr.2024.102514","DOIUrl":"10.1016/j.jspr.2024.102514","url":null,"abstract":"<div><div>The cell wall degradation results in firmness loss thus negatively affecting the overall eating quality. So, in this work, the influence of carrageenan [0, 0.5, 1.0 and 1.5% (w/v)] coating was studied on ‘Shamber’ grapefruit during storage at 20 ± 1 °C for 42 days (d). The 1.5% carrageenan concentration suppressed metabolic activities and reduced disease rate (about 41%), weight loss (around 46%), malondialdehyde, electrolytes leakage, superoxide anion and hydrogen peroxide. The higher antioxidant enzyme activity as well as total flavonoids, ascorbic acid and total phenolic contents were noted in 1.5% treatment. The 1.5% coating delayed the increase in water-soluble pectin (WSP) and suppressed the reduction of sodium carbonate soluble pectin (NCSP), protopectin, chelate soluble pectin (CSP), hemicellulose and cellulose content. The grapefruit coated with 1.5% carrageenan had lower activities of cellulase (CEL), polygalacturonase (PG), β-galactosidase (β-gal) and pectin methylesterase (PME) enzyme along with higher firmness. The 1.5% carrageenan preserved higher titratable acidity and reduced increase in total soluble solids and ripening index in coated grapefruit. In conclusion, 1.5% carrageenan coating could be considered appropriate for delaying the quality deterioration in grapefruit.</div></div>","PeriodicalId":17019,"journal":{"name":"Journal of Stored Products Research","volume":"111 ","pages":"Article 102514"},"PeriodicalIF":2.7,"publicationDate":"2024-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143104466","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Towards food security with the Grain Shield web application for stored grain pest identification

IF 2.7 2区农林科学 Q1 ENTOMOLOGY

Journal of Stored Products Research

Pub Date : 2024-12-12 DOI: 10.1016/j.jspr.2024.102515

Muthukrishnan Sakthivel Santhanambika, Gopal Maheswari

As an agroeconomical country, grain storage in India is integral to the farmer's society. A significant portion of grains are lost to biotic and abiotic factors, out of which insect pests impact food security and economic stability. The preliminary step in mitigating these post-harvest losses is to identify the pest that has infested the grains. Hence, it is essential to find a solution in managing these pests to meet the nutritional demands of the burgeoning human population. In the era of digital advancement, artificial intelligence (AI) has made significant strides across various fields. This study delves into the potential of transfer learning (TL) as a tool for reducing grain losses by identifying pests infesting stored grains. Pre-trained models like VGG16, VGG19, InceptionV3, and Xception were employed and fine-tuned for pest recognition. The performance of these models was evaluated using metrics and a confusion matrix. The accuracy of the models VGG16, VGG19, InceptionV3, and Xception were 99.8%, 99.2%, 93%, and 95.8%, respectively, whereas VGG16 achieved superior performance in accuracy, precision, recall, and F1-score metrics. Confusion matrices provided insights into model strengths and weaknesses, emphasizing the importance of architecture and training data quality. Leveraging these findings, a web tool using Flask (Python) powered by VGG16 was developed to assist farmers in pest identification. This research demonstrates the potential of Transfer learning for combating post-harvest losses and promoting food security.

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引用次数: 0

New inlet duct arrangements for grain aeration in a small-scale silo: Computational simulation and experimental validation

IF 2.7 2区农林科学 Q1 ENTOMOLOGY

Journal of Stored Products Research

Pub Date : 2024-12-12 DOI: 10.1016/j.jspr.2024.102511

Dariush Zare , Naser Razavizadeh , Kamran Maleki-Majd

The arrangement of air inlet ducts is a crucial factor influencing the temperature distribution within grain storage systems. Poorly designed ducts can result in uneven temperature distribution throughout the grain bulk, leading to localized conditions that promote fungal and insect growth, while also increasing the power consumption of the fan needed for aeration. This study aimed to evaluate the temperature distribution within bulk rough rice during aeration, using both numerical simulations and experimental methods, for three innovative inlet air duct arrangements -E, F, and H-each resembling the respective letter's shape. The study was conducted on a scaled-down model of a one-fourteenth real-size on-farm silo. Temperature data were collected at various locations within the grain bed, and the simulation outcomes were validated by comparing them to the experimental data. The results indicate that, for all three air inlet arrangements, the temperature distribution in both cross-sectional planes (parallel and perpendicular to the airflow direction) was uniform by the end of the aeration process. The time required to reach a temperature of 15.5 °C in both planes was approximately the same, highlighting the effective performance of these arrangements in different orientations. Among the duct arrangements in the experimental silo, the F-duct arrangement demonstrated superior ventilation performance, achieving the target temperature at least %25 faster than the E and H arrangements. The higher efficiency is attributed to its larger volumetric heat transfer coefficient. The H duct arrangement, however, offers other advantages, including a pressure drop that is at least 30% lower and a minimum 17% reduction in power consumption within the storage bed compared to the F and E duct configurations.

The numerical simulations closely aligned with the experimental data. The relative mean error values for volume flow rates of 0.0064, 0.014, and 0.018 m³.s⁻¹ ranged between 2 and 3.53%, 2.77–4.98%, and 2.42–8.52%, respectively. These results confirm the model's accuracy and underscore its potential as a valuable tool for optimizing silo inlet duct designs.

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引用次数: 0

Enhanced wheat flour safety through impact treatment: Effective control of Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae)

IF 2.7 2区农林科学 Q1 ENTOMOLOGY

Journal of Stored Products Research

Pub Date : 2024-12-10 DOI: 10.1016/j.jspr.2024.102513

Fuqiang Gao, Qianqian Zhang, Alkassoumi Hassane Hamadou, Bin Xu

Pests such as Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae) significantly threaten the storage quality of wheat flour, posing substantial preservation challenges. This study evaluated the effectiveness of impact insecticidal device on T. castaneum in wheat flour. Response surface methodology (RSM) was used to optimize the impacts of treatment parameters on wheat flour, and the quality changes examined. Multiple-response optimization indicated that a feed rate (6 t/h), rotor speed (3500 r/min, ∼3400×g) and 2 impact treatments represented the optimal condition. Under these conditions, the survival rate of insect eggs in wheat flour was zero, with 41.33 fragments >0.15 mm, 11.67 fragments >0.20 mm, and a damaged starch content of 23.37 UCDc. The application of optimized parameters to infected wheat flour resulted in similar quality as compared to untreated sample. Further increasing the impact intensity would affect its processing properties. This research demonstrated the potentiality to achieve zero pest presence in wheat flour using impact insecticidal device while maintaining acceptable flour quality, thus providing a promising solution for the flour processing industry.

{"title":"Enhanced wheat flour safety through impact treatment: Effective control of Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae)","authors":"Fuqiang Gao, Qianqian Zhang, Alkassoumi Hassane Hamadou, Bin Xu","doi":"10.1016/j.jspr.2024.102513","DOIUrl":"10.1016/j.jspr.2024.102513","url":null,"abstract":"<div><div>Pests such as <em>Tribolium castaneum</em> (Herbst) (Coleoptera: Tenebrionidae) significantly threaten the storage quality of wheat flour, posing substantial preservation challenges. This study evaluated the effectiveness of impact insecticidal device on <em>T. castaneum</em> in wheat flour. Response surface methodology (RSM) was used to optimize the impacts of treatment parameters on wheat flour, and the quality changes examined. Multiple-response optimization indicated that a feed rate (6 t/h), rotor speed (3500 r/min, ∼3400×<em>g</em>) and 2 impact treatments represented the optimal condition. Under these conditions, the survival rate of insect eggs in wheat flour was zero, with 41.33 fragments >0.15 mm, 11.67 fragments >0.20 mm, and a damaged starch content of 23.37 UCDc. The application of optimized parameters to infected wheat flour resulted in similar quality as compared to untreated sample. Further increasing the impact intensity would affect its processing properties. This research demonstrated the potentiality to achieve zero pest presence in wheat flour using impact insecticidal device while maintaining acceptable flour quality, thus providing a promising solution for the flour processing industry.</div></div>","PeriodicalId":17019,"journal":{"name":"Journal of Stored Products Research","volume":"111 ","pages":"Article 102513"},"PeriodicalIF":2.7,"publicationDate":"2024-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143104463","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0