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Chemical characterization of Cantharellus cibarius and Lactarius piperatus methanolic extracts – potential supplements in laboratory rearing of Plodia interpunctella (Lepidoptera: Pyralidae) 红斑蝥和胡椒乳虱甲醇提取物的化学特性及其在实验室饲养斑间Plodia(鳞翅目:蚜科)中的潜在补充物
IF 2.7 2区 农林科学 Q1 ENTOMOLOGY Pub Date : 2025-11-29 DOI: 10.1016/j.jspr.2025.102903
Dragana Z. Predojević , Nevena N. Petrović , Tomislav B. Tosti , Filip N. Vukajlović , Marijana M. Kosanić , Snežana B. Pešić
Dietary supplements are concentrated sources of vitamins, minerals, or other substances with nutritional or physiological effects intended to supplement the regular diet. The aim of this study was the chemical characterization of methanolic extracts from two edible mushrooms, Cantharellus cibarius Fr. (1821) (Cantharellaceae) and Lactarius piperatus (L.) Roussel (1806) (Russulaceae), and the investigation of their potential use as dietary supplements in the rearing of Plodia interpunctella (Hübner, 1813) (Lepidoptera: Pyralidae) laboratory populations. This was examined by assessing the effect of the extracts on survival rate and life history parameters of P. interpunctella: mean developmental duration (MDD), adult emergence dynamics, longevity and fecundity on maize kernels. The attractant potential of the mushroom extracts was also investigated, by examining their influence on the oviposition preference of mated females. The highest number of emerged adults was recorded with 1 % L. piperatus extract (46.5 ± 5.27). Significantly shorter MDD was observed with 5 % C. cibarius (32.48 ± 0.53) and 5 % L. piperatus (32.73 ± 0.41) extract treatments, compared to the control. Adult emergence lasted significantly longer in the treatments (15.75 ± 0.25), compared to the control. The highest fecundity was observed with 5 % L. piperatus extract treatment (163.38 ± 10.21). The oviposition preference test confirmed that treatments with 2 % C. cibarius extracts are attractant, while L. piperatus extracts have no attractant potential. Chemical analysis of both extracts was performed, including qualitative and quantitative analysis of polyphenols, analysis of mineral elements, and determination of anions of organic acids, sugars and sugar alcohols, confirming a high nutrient content.
膳食补充剂是维生素、矿物质或其他具有营养或生理作用的物质的浓缩来源,旨在补充常规饮食。本文研究了两种食用菌Cantharellus cibarius Fr. (1821) (Cantharellaceae)和Lactarius piperatus (L.)的甲醇提取物的化学性质。Roussel (1806) (Russulaceae),以及它们作为膳食补充剂在饲养斑间Plodia interpunctella (h bner, 1813)(鳞翅目:Pyralidae)实验室种群中的潜在用途的研究。通过测定不同提取物对棉铃虫成虫存活率和生活史参数(平均发育期、成虫羽化动态、寿命和玉米籽粒繁殖力)的影响来验证这一点。通过观察其对交配雌性产卵偏好的影响,研究了香菇提取物的引诱电位。以1%胡椒柳提取物的出蛹率最高(46.5±5.27)只。5% cibarius(32.48±0.53)和5% L. piperatus(32.73±0.41)提取物组的MDD显著短于对照组。处理组成虫羽化时间显著长于对照组(15.75±0.25)。5%花椒提取液处理的繁殖力最高(163.38±10.21)。产卵偏好试验结果表明,2%毒姬鼠提取物有引诱作用,而油梨提取物无引诱作用。对两种提取物进行了化学分析,包括多酚的定性和定量分析,矿物元素的分析,有机酸、糖和糖醇的阴离子的测定,证实了其高营养含量。
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引用次数: 0
Peptides from Leptogenys processionalis exhibit Antimicrobial activity against key pathogens of postharvest pomegranate (Punica granatum L.) 对石榴采后主要病原菌的抑菌活性研究
IF 2.7 2区 农林科学 Q1 ENTOMOLOGY Pub Date : 2025-11-29 DOI: 10.1016/j.jspr.2025.102894
B.N. Ramesh , C.V. Babu , S.D. Pradeep , E. Dilip , P.A. Niveditha
Antimicrobial peptides (AMPs) are eco-friendly alternatives to chemical pesticides due to their broad-spectrum activity and reduced risk of resistance. Their role in postharvest disease control of fruits such as pomegranate (Punica granatum L.) is still largely unexplored. In this study, crude AMPs from the predatory ant Leptogenys processionalis were evaluated for antibacterial and antifungal activity. The crude extract showed strong antibacterial effects against Escherichia coli, Staphylococcus aureus, and Xanthomonas spp., with inhibition zones up to 50 ± 1.22 mm2 in diameter at 70 μg/disk, and concentration-dependent antifungal activity against Alternaria alternata, reaching 94 ± 0.07 % inhibition at 500 μg/mL. RP-HPLC fractionation yielded three fractions, of which Fraction 1 had the highest protein concentration (2.09 mg/mL) and displayed maximum activity, producing inhibition zones up to 84.72 ± 1.60 mm2 in diameter at 300 μg/disk and 91.07 ± 0.37 % inhibition of Alternaria. alternata at 500 μg/mL. MALDI-TOF MS analysis revealed prominent peptide peaks, including one at 1794.13 m/z, probably responsible for antimicrobial effects. Importantly, fruit assays demonstrated that crude extract at 300 μg/mL achieved 98 ± 0.4 % inhibition, statistically equivalent to commercial fungicide treatment (97 ± 0.5 %). These results identify L. processionalis as a novel AMP source with strong potential for sustainable postharvest disease management in pomegranate.
抗菌肽(AMPs)具有广谱活性和较低的抗性风险,是化学农药的环保替代品。它们在石榴(Punica granatum L.)等水果采后病害防治中的作用仍未得到充分研究。本研究对掠食性蚂蚁(Leptogenys processionalis)的抗菌和抗真菌活性进行了评价。粗提物对大肠杆菌、金黄色葡萄球菌和黄单胞菌均有较强的抑菌作用,在70 μg/盘时抑菌带直径可达50±1.22 mm2,对交替孢霉的抑菌活性呈浓度依赖性,在500 μg/mL时抑菌率可达94±0.07%。反相高效液相色谱(RP-HPLC)分离得到3个部分,其中第1部分蛋白浓度最高(2.09 mg/mL),活性最高,在300 μg/盘时抑制区直径达84.72±1.60 mm2,抑制率为91.07±0.37%。交替剂量500 μg/mL。MALDI-TOF MS分析显示了突出的肽峰,其中一个位于1794.13 m/z,可能与抗菌作用有关。重要的是,水果试验表明,300 μg/mL粗提取物的抑制率为98±0.4%,与商业杀菌剂处理(97±0.5%)相当。这些结果表明,processionl是一种新的AMP来源,对石榴采后病害的可持续管理具有很强的潜力。
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引用次数: 0
Recent technological innovations and strategies for reducing post-harvest losses during bulk grain storage: Applications of IoT and non-destructive quality evaluation 减少散装粮食储存收获后损失的最新技术创新和策略:物联网和无损质量评估的应用
IF 2.7 2区 农林科学 Q1 ENTOMOLOGY Pub Date : 2025-11-29 DOI: 10.1016/j.jspr.2025.102893
Pratibha Jain , Mohammed Shafiq Alam , Manpreet Kaur Saini , Raouf Aslam
Ensuring food security, minimizing post-harvest losses, and improving grain storage methods are crucial for sustaining agricultural production. Currently, grain storage mismanagement contributes to significant losses, especially in developing countries. This review provides an in-depth analysis of global grain storage systems, traditional and modern technologies, and the critical factors influencing grain quality. It provides a recent report on the quantitative and qualitative losses caused by environmental factors, biological agents, and socio-economic conditions. Moreover, the study examines advancements in storage technologies, such as hermetic storage as a part of dry chain, bulk systems, and the integration of IoT for monitoring grain storage conditions. Through this comprehensive overview, the review offers solutions to optimize storage practices and reduce food losses, thereby contributing to food security and economic sustainability.
确保粮食安全、尽量减少收获后损失和改进粮食储存方法对维持农业生产至关重要。目前,粮食储存管理不善造成了重大损失,特别是在发展中国家。本文对全球粮食储存系统、传统与现代技术以及影响粮食品质的关键因素进行了深入分析。它提供了一份关于环境因素、生物制剂和社会经济条件造成的数量和质量损失的最新报告。此外,该研究还考察了存储技术的进步,例如作为干链一部分的密封存储、散装系统以及用于监测粮食存储条件的物联网集成。通过这一全面概述,本报告提出了优化储存做法和减少粮食损失的解决方案,从而促进粮食安全和经济可持续性。
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引用次数: 0
Feeding preference of Sitophilus oryzae (L) (Coleoptera: Curculionidae) on dried noodles stored at varied temperature and humidity: The perspective of volatile components 米象蛾(鞘翅目:弯蝇科)对不同温度和湿度条件下的干面的取食偏好:挥发性成分的研究
IF 2.7 2区 农林科学 Q1 ENTOMOLOGY Pub Date : 2025-11-28 DOI: 10.1016/j.jspr.2025.102899
Qianqian Zhang, Lei Shen, Shuyi Liu, Yajing Qi, Wenshen He, Liming Yue, Bin Xu
Dried noodles, as typical cereal-based foods, are susceptible to insect infestation during storage, such as Sitophilus oryzae (L.) (Coleoptera: Curculionidae), which could cause deterioration of food quality and may endanger public health. This study investigated the relationship between the volatile components of dried noodles under different temperature and humidity conditions and the feeding preference of S. oryzae. S. oryzae showed a greater preference for dried noodles from low-refined white flour (N3). Changes in the volatile compounds of dried noodles, caused by temperature and humidity, affected the feeding preference of S. oryzae. The attraction of dried noodles to S. oryzae was mitigated under 20 °C storage conditions, and dried noodles stored at 32 °C, 55 % RH, and 65 % RH even exhibited repellent effects against the S. oryzae during the later stages of storage. The content of volatile components was the highest in dried noodles stored at 75 %RH. Humidity had a greater effect than temperature on the content of volatile components in dried noodles. 2,8,8-trimethyldecane and eicosane were the key components in the feeding preference of S. oryzae. S. oryzae showed negative behavioural responses to 2-methyloctan-1-ol, 2-ethylhexyl N-propyl carbamate, 9-octylheptadecane, and hexadecyl nonyl ether‌. The results showed that keeping the ambient temperature below 20 °C and 55 % RH can reduce the exogenous infestation of S. oryzae in dried noodles. These findings provide a new idea for the precise control of pests during the storage of dried noodles and other cereal products, as well as the application of food volatiles as potential attractants or repellents.
干面作为典型的谷类食品,在贮存过程中容易受到米象(Sitophilus oryzae, L.)(鞘翅目:蚊科),会导致食物品质恶化,危害公众健康。研究了不同温度和湿度条件下干面挥发性成分与米曲菌取食偏好的关系。米曲霉对低精制白面粉制成的干面条表现出更大的偏好(N3)。温度和湿度引起的干面挥发性物质的变化影响了米曲菌的取食偏好。在20℃的贮藏条件下,干面对米曲菌的吸引力减弱,在32℃、55% RH和65% RH的贮藏条件下,干面在贮藏后期甚至表现出对米曲菌的排斥作用。在75% RH条件下,挥发性成分含量最高。湿度对干面挥发性成分含量的影响大于温度。2,8,8-三甲基癸烷和廿烷是影响米曲霉取食偏好的关键成分。米曲霉对2-甲基辛烷-1-醇、2-乙基己基氨基甲酸酯、9-辛基十七烷和十六烷基壬基醚表现出负反应。结果表明,保持环境温度在20℃以下,相对湿度在55%以下,可以减少米曲菌在干面中的外源侵染。这些研究结果为干面等谷物产品贮藏过程中害虫的精确控制以及食品挥发物作为潜在引诱剂或驱避剂的应用提供了新的思路。
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引用次数: 0
Impact of factitious food on the life table, functional and numerical response parameters of Blaptostethus pallescens Poppius (hemiptera: Anthocoridae) 人为食物对淡纹蠓生命表、功能及数值响应参数的影响(半翅目:蚁科)
IF 2.7 2区 农林科学 Q1 ENTOMOLOGY Pub Date : 2025-11-27 DOI: 10.1016/j.jspr.2025.102888
Mayur Deepak Wabhitkar , Manmeet Brar Bhullar , Arshpreet Singh , Azadeh Farazmand , Shiwani Sharma , Jenia Thakur
The present study evaluates the life table, functional and numerical response of Blaptostethus pallescens (Poppius) when reared on three different factitious preys: Tyrophagus putrescentiae (Schrank), Corcyra cephalonica (Stainton), and Phthorimaea operculella (Zeller). Significant variation was observed in developmental time, adult longevity, and reproductive parameters across the three diets. Individuals fed on T. putrescentiae exhibited prolonged pre-adult development and reduced adult longevity and fecundity compared to those fed on lepidopteran eggs. Female fecundity was highest on C. cephalonica and P. operculella eggs, while lowest on T. putrescentiae. Life table analysis revealed that the intrinsic rate of increase (r) was significantly lower on T. putrescentiae, indicating suboptimal population growth, whereas lepidopteran egg diets promoted higher reproductive and population parameters. Functional response analysis confirmed a type II response across all prey, with the highest attack rate (3.5 h−1) and shortest handling time (0.0079 h) recorded on T. putrescentiae. Despite lower fecundity, T. putrescentiae supported full development and survival of B. pallescens, suggesting its potential as a factitious prey in mass rearing systems. This predator completed its life cycle inside breadboxes containing wheat bran and yeast, indicating feasibility for use in predatory sachets. These findings support the use of B. pallescens in biological control programs, particularly in greenhouse systems, where cost-effective and locally adaptable rearing methods are urgently needed. The study also highlights the promise of T. putrescentiae as a prey species for slow-release systems, offering a practical solution for enhancing macrobial biocontrol strategies in India. Although T. putrescentiae is known to produce allergens, the adoption of simple safety measures, such as wearing masks and gloves can effectively minimize any associated risks during handling or rearing.
本研究评估了三种不同的人工饵料:腐Tyrophagus purescentiae (Schrank)、头颈Corcyra cephalonica (Stainton)和有盖Phthorimaea operculella (Zeller)饲养时,灰鳞Blaptostethus pallescens (Poppius)的生命表、功能和数值反应。三种饮食在发育时间、成虫寿命和生殖参数上观察到显著差异。与以鳞翅目卵为食的个体相比,以腐翅虫为食的个体表现出较长的成虫前发育时间和较短的成虫寿命和繁殖力。雌虫的产卵能力以头孢丝虫和盖孢丝虫的卵最高,而以腐殖丝虫的卵最低。生命表分析显示,腐斑蝶的内在增长率(r)明显较低,表明种群增长不理想,而鳞翅目的卵饮食促进了更高的繁殖和种群参数。功能反应分析证实所有猎物均为II型反应,腐尸的攻击率最高(3.5 h−1),处理时间最短(0.0079 h)。尽管繁殖力较低,但腐尸螟支持白僵菌的完全发育和存活,表明其在大规模饲养系统中有作为人工猎物的潜力。这种捕食者在含有麦麸和酵母的面包盒中完成其生命周期,这表明在捕食小袋中使用的可行性。这些发现支持在生物防治计划中使用白僵菌,特别是在温室系统中,迫切需要具有成本效益和适合当地的饲养方法。该研究还强调了腐尸霉作为缓释系统猎物的前景,为加强印度的微生物生物防治策略提供了切实可行的解决方案。虽然已知腐殖弓形虫会产生过敏原,但采取简单的安全措施,如戴口罩和手套,可以有效地减少处理或饲养过程中的任何相关风险。
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引用次数: 0
Legume seed macronutrient composition influences tri-trophic host–parasitoid dynamics in a stored-legume system 豆科植物种子常量营养成分对贮藏系统中三营养寄主-寄生蜂动态的影响
IF 2.7 2区 农林科学 Q1 ENTOMOLOGY Pub Date : 2025-11-26 DOI: 10.1016/j.jspr.2025.102900
Soumaya Haouel-Hamdi , Nada Abdelkader , Emna Boushih , Jouda Mediouni-Ben Jemâa
This study investigated the tritrophic interactions among legume host plants, the bruchid pest Callosobruchus maculatus, and its parasitoid Dinarmus basalis, emphasizing the effects of biochemical composition on parasitoid performance. C. maculatus strains developing on protein and carbohydrate rich seeds were associated with higher body weight, large size, and improved biological parameters, including longevity, adult emergence rates, and fertility. Female fecundity and egg viability in C. maculatus were positively correlated with larval protein and carbohydrate intake, reflecting the quality of the host seed resource. Parasitoid fitness, essential for effective biological control of host populations, depends critically on substantial protein, lipid, and carbohydrate acquisition for survival and reproductive success. Our focus was specifically on the correlations between adult parasitoid life history parameters and host biochemical composition. The sugar content of fourth-instar C. maculatus larvae significantly influenced parasitoid performance, with a notable positive relationship between larval sugar concentration and D. basalis sex ratio bias toward females. This represents the first comprehensive investigation of relationships between the biochemical composition of C. maculatus L4 larvae and the biological and demographic performance of its parasitoid D. basalis. Our results demonstrate that macronutrient content in both plant hosts and herbivorous pests plays a crucial role in determining the strength and outcomes of tritrophic interactions.
本研究研究了豆科寄主植物、毛糙蛾及其拟寄生物基底螯蟹之间的营养相互作用,重点研究了生化组成对拟寄生物性能的影响。在富含蛋白质和碳水化合物的种子上发育的黄斑弧菌菌株具有更高的体重、更大的体型和更好的生物学参数,包括寿命、成虫羽化率和繁殖力。黄斑夜蛾的雌虫繁殖力和卵活力与幼虫蛋白质和碳水化合物摄入量呈正相关,反映了寄主种子资源的质量。拟寄生物的适应性对寄主种群的有效生物控制至关重要,它主要依赖于大量蛋白质、脂质和碳水化合物的获取,以维持生存和繁殖成功。我们的重点是研究成虫生活史参数与寄主生化组成的相关性。4龄黄斑夜蛾幼虫含糖量对寄生蜂的寄生性能有显著影响,且含糖量与基底夜蛾的雌偏性率呈显著正相关。这是第一次全面研究斑夜蛾L4幼虫的生化组成与其寄生蜂基底夜蛾的生物学和人口统计学表现之间的关系。我们的研究结果表明,植物宿主和草食性害虫体内的宏量营养素含量在决定营养相互作用的强度和结果方面起着至关重要的作用。
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引用次数: 0
Resistance grade of different Triticum species toward Sitophilus oryzae L. (Coleoptera: Curculionidae) 不同小麦品种对米象虫的抗性等级(鞘翅目:粟象科)
IF 2.7 2区 农林科学 Q1 ENTOMOLOGY Pub Date : 2025-11-26 DOI: 10.1016/j.jspr.2025.102892
Salvatore Guarino , Shaun Gasparro , Giuliano Cerasa , Antonioni Acacio Campos Moliterno , Rosolino Ingraffia , Antonella Lo Porto , Giacomo Gargano , Paolo Ruisi , Dario Giambalvo , Ezio Peri
Varietal resistance in cereals represents a promising and sustainable strategy for managing biotic stressors, including insect pests during storage. In this study, eighteen genotypes from six cereal species - Triticum monococcum, T. dicoccum, T. spelta, T. durum, T. aestivum, and Triticosecale - were evaluated for their resistance to the rice weevil, Sitophilus oryzae L., one of the most destructive stored product pests. The objective was to assess differences in susceptibility by measuring some insect parameters, such as adult mortality, F1 progeny production and body size, along with grain traits such as kernel hardness and weight. For this scope, bioassays included both non-choice and two-way choice tests to evaluate insect performance and preference. Significant inter- and intra-specific variation in susceptibility was observed. In non-choice bioassays, Sitophilus oryzae generally showed higher adult mortality and lower adult progeny emergence feeding and ovipositing on T. durum and T. dicoccum genotypes, suggesting greater resistance of these genotypes; on the contrary T. monococcum and triticale appear more susceptible. Grain hardness was strongly and negatively correlated with adult progeny output, supporting its role as a mechanical barrier to larval development. However, kernel hardness did not influence adult body size, suggesting that individuals overcoming this barrier can still complete normal development. Kernel weight showed a positive correlation with female size, indicating a potential fitness advantage for the insects feeding on larger seeds. In two-choice assays, S. oryzae showed a preference for softer genotypes, particularly triticale Agostino and Tribeca, over harder genotypes such as durum wheat Perciasacchi and Aureo. These results provide new insights into the resistance potential of traditional and modern Triticum genotypes. The integration of resistant genotypes, especially from T. durum and T. dicoccum, into breeding programs and storage systems may contribute to reducing chemical inputs and improving long-term grain protection strategies.
谷物的品种抗性是一种有前途的可持续策略,可用于管理生物应激源,包括储存期间的害虫。本研究对6种谷物(单粒小麦、双粒小麦、粗粒小麦、硬粒小麦、aestivum小麦和小黑麦)18个基因型对稻谷象甲(稻谷象甲是最具破坏性的储藏产品害虫之一)的抗性进行了评价。目的是通过测量一些昆虫参数,如成虫死亡率、F1后代产量和体型,以及籽粒硬度和重量等谷物性状,来评估敏感性的差异。在这个范围内,生物测定包括非选择试验和双向选择试验,以评估昆虫的性能和偏好。观察到显着的种间和种内易感性差异。在非选择性生物测定中,米象蚜对硬粒稻和二粒稻均表现出较高的成虫死亡率和较低的成虫后代羽化、摄食和产卵,表明这两种基因型对米象蚜具有较强的抗性;相反,单球菌和小黑麦更易感染。籽粒硬度与成虫子代产量呈显著负相关,支持其作为幼虫发育的机械屏障的作用。然而,果仁硬度并不影响成虫的体型,这表明克服这个障碍的个体仍然可以完成正常的发育。籽粒重与雌虫体型呈正相关,表明以较大的种子为食的昆虫具有潜在的适应性优势。在两种选择试验中,稻谷球菌表现出对软基因型的偏爱,特别是小黑麦Agostino和Tribeca,而不是硬基因型,如硬粒小麦Perciasacchi和Aureo。这些结果为传统和现代小麦基因型的抗性潜力提供了新的见解。将抗性基因型,特别是来自硬粒稻和二粒稻的抗性基因型整合到育种计划和储存系统中,可能有助于减少化学品投入和改善长期粮食保护策略。
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引用次数: 0
Impact of commercial phosphine fumigation of stored-grains in concrete silos on survival and progeny of natural free-living insect pests 商品磷化氢熏蒸混凝土仓仓储粮对天然散居害虫存活及后代的影响
IF 2.7 2区 农林科学 Q1 ENTOMOLOGY Pub Date : 2025-11-26 DOI: 10.1016/j.jspr.2025.102895
Petar Kljajić, Goran Andrić, Marijana Pražić Golić
This study was conducted to examine the impact of commercial phosphine fumigation on survival and progeny of natural free-living insect populations in wheat and maize stored in concrete silos. Aluminium phosphide pellets (560 g/kg) were applied at the recommended dose of 20–25 pellets/t of grains (4–5 g of phosphine gas/t) in two airtight cells in one silo at Pančevo locality (wheat and maize) and in one without airtight cell, in silos in Perlez (wheat) and Vrbas (maize). Samples collected before fumigation were used for pre-treatment assessment of grains' infestation levels, while samples taken for post-treatment assessment served to determine insect survival after 4 weeks and progeny after 8, 12 and 16 weeks. All taken samples were kept and analysed in laboratory. In pre-treatment assessment of wheat we found two primary and three secondary pests in density rates 0–0.9 and 1-34 adults/sample/kg, respectively. In maize were recorded one primary and three secondary pests in rates 1.5–1.7 and 0.9–62 adults/sample/kg, respectively. Post-treatment assessments of treated grains showed no insect survival and progeny. However, in untreated grains we recorded population growth of all insect species, especially of primary pests, Rhyzopertha dominica (F.) in wheat in Pančevo, from 0 to 170.4 adults/kg, and Sitophilus zeamais (Motsch.), in maize in Pančevo from 1.5 to 82.6 adults/kg and in Vrbas from 1.7 to 74.9 adults/kg. The phosphine gas high impact on survival and progeny of all recorded natural free-living insects, regardless of grain and storage type is promising for its further use in the future.
本研究旨在研究商业磷化氢熏蒸对混凝土筒仓储存的小麦和玉米自然自由生活昆虫种群的存活和后代的影响。磷化铝颗粒(560 g/kg)按20-25粒/吨谷物(4-5克磷化气/吨)的推荐剂量施用于pan evo地区(小麦和玉米)一个仓内的两个密闭仓内和Perlez(小麦)和Vrbas(玉米)仓内的一个不密闭仓内。熏蒸前采集的样品用于处理前评估谷物侵染水平,处理后评估的样品用于测定4周后的昆虫存活率和8、12和16周后的后代。所有采集的样品均保存在实验室进行分析。在小麦处理前评估中,主要害虫2只,次要害虫3只,密度率分别为0 ~ 0.9和1 ~ 34只/样/kg。在玉米中主要害虫1只,次要害虫3只,危害率分别为1.5 ~ 1.7和0.9 ~ 62只/样/kg。处理后的评估显示,处理后的谷物没有昆虫存活和后代。然而,在未经处理的谷物中,我们记录了所有昆虫的种群增长,特别是主要害虫,在pan evo小麦中的Rhyzopertha (F.)在0到170.4成虫/kg之间,在pan evo玉米中的玉米象(Motsch.)在1.5到82.6成虫/kg之间,在Vrbas中在1.7到74.9成虫/kg之间。磷化氢气体对所有有记录的自然自由生活昆虫的生存和后代都有很高的影响,无论粮食和储存类型如何,它在未来的进一步利用是有希望的。
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引用次数: 0
Behavioural response of Sitophilus zeamais to essential oil-derived compounds 玉米象对精油衍生化合物的行为反应
IF 2.7 2区 农林科学 Q1 ENTOMOLOGY Pub Date : 2025-11-25 DOI: 10.1016/j.jspr.2025.102898
Inês Gonçalves de Sousa , Pedro Naves , Graça Barros , António Mexia , Carla Brites
Insect infestations in stored grain systems pose a significant threat to global food security by reducing both the quality and quantity of cereals. Sitophilus zeamais, the maize weevil, is one of the most destructive pests affecting stored cereals worldwide. While synthetic chemicals are commonly used for control, concerns regarding environmental safety, human health and the emergence of insecticide resistance have intensified the search for alternative control strategies. Essential oils and their constituents have emerged as promising eco-friendly alternatives. In this study, we evaluated the behavioural responses of S. zeamais to three isolated high-purity essential oil-derived compounds – 1,8-cineole, (S)-(−)-Limonene, and Eugenol – using a four-arm olfactometer. Adult S. zeamais were exposed to different concentrations of each compound, and repellence was inferred from the time spent in the central or distal zones of the olfactometer. The essential oil-derived compounds tested demonstrated promising repellent properties against weevils, as the insects spent more time away from the source or remained in the central region of the device, indicating repellent activity. Our findings contribute to the expanding body of research on botanical pest control methods and underscore the potential of these compounds to support the development of sustainable and environmentally friendly pest management strategies. These approaches can reduce dependency on synthetic chemicals while effectively limiting post-harvest losses in stored grains.
储粮系统中的虫害降低了谷物的质量和数量,对全球粮食安全构成重大威胁。玉米象甲(Sitophilus zeamais)是影响全球储藏谷物的最具破坏性的害虫之一。虽然合成化学品通常用于控制,但对环境安全、人类健康和杀虫剂抗药性出现的关切促使人们加紧寻找其他控制战略。精油及其成分已成为有希望的环保替代品。在这项研究中,我们使用四臂嗅觉仪评估了玉米玉米对三种分离的高纯度精油衍生化合物- 1,8-桉叶油素,(S)-(−)-柠檬烯和丁香酚-的行为反应。将玉米玉米螟成虫暴露于不同浓度的化合物中,并根据其在嗅觉仪中心或远端区域停留的时间推断出其驱避性。经测试的精油衍生化合物显示出对象鼻虫有良好的驱避性能,因为昆虫在远离源或留在装置的中心区域的时间更长,表明驱避活性。我们的发现有助于扩大植物害虫控制方法的研究范围,并强调这些化合物的潜力,以支持可持续和环境友好型害虫管理策略的发展。这些方法可以减少对合成化学品的依赖,同时有效地限制储存谷物的收获后损失。
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引用次数: 0
Efficient and lightweight deep learning model for small-object detection in stored grain pest management 储粮病虫害管理中小目标检测的高效轻量级深度学习模型
IF 2.7 2区 农林科学 Q1 ENTOMOLOGY Pub Date : 2025-11-15 DOI: 10.1016/j.jspr.2025.102890
Fuyan Sun , Zhizhong Guan , Zongwang Lyu , Tong Zhen , Shanshan Liu , Xiongfei Li
Timely and accurate pest detection in grain storage environments is essential for safeguarding food security. Traditional detection methods face significant challenges in efficiently and precisely identifying small pests. This study presents Pest-YOLO, a lightweight detection model based on YOLOv8n, specifically designed for stored grain pest identification. The model incorporates an Efficient Feature Enhancement Module (EFEM) that efficiently captures pixel-level details while reducing computational redundancy. A Detail-Enhanced YOLO Head (DEYH) is designed to improve computational efficiency through shared convolutional layers. Furthermore, an Attention-Based Intra-Scale Feature Interaction (AIFI) module is integrated into the backbone network to enhance global feature interactions, improving the overall fine-grained feature extraction capability. Additionally, the network structure is optimized by introducing a P2 detection layer while removing the P5 layer, thereby enhancing small-object detection accuracy while reducing computational overhead. The model was trained and evaluated on a custom Stored Grain Pest 5 (SGP5) dataset containing 8000 images of five common pest species. Experimental results demonstrate that Pest-YOLO achieves 95.0 % precision and 93.6 % recall, with mAP0.5 and mAP0.5:0.95 reaching 97.5 % and 60.0 %, respectively. With only 1.3M parameters and 4.7G FLOPs, the model demonstrates superior computational efficiency while maintaining high detection accuracy. When deployed on the NVIDIA Jetson Orin Nano edge computing platform, Pest-YOLO achieves real-time inference at 36.2 FPS, validating its practical applicability in resource-constrained environments. This research provides a practical solution for intelligent grain storage management and accurate pest identification.
在粮食储存环境中及时、准确地检测有害生物对保障粮食安全至关重要。传统的检测方法在有效、准确地识别小害虫方面面临重大挑战。本研究提出了一种基于YOLOv8n的轻量化检测模型pest - yolo,专门用于储粮害虫鉴定。该模型采用高效特征增强模块(EFEM),有效捕获像素级细节,同时减少计算冗余。细节增强YOLO Head (DEYH)通过共享卷积层来提高计算效率。此外,在骨干网中集成了基于注意力的尺度内特征交互(AIFI)模块,增强了全局特征交互,提高了整体细粒度特征提取能力。此外,通过在去掉P5层的同时引入P2检测层来优化网络结构,从而在提高小目标检测精度的同时降低了计算开销。该模型在一个定制的存储谷物害虫5 (SGP5)数据集上进行训练和评估,该数据集包含5种常见害虫的8000张图像。实验结果表明,Pest-YOLO的准确率为95.0%,召回率为93.6%,mAP0.5和mAP0.5:0.95分别达到97.5%和60.0%。该模型仅使用1.3M个参数和4.7G FLOPs,在保持较高检测精度的同时,具有优越的计算效率。当部署在NVIDIA Jetson Orin Nano边缘计算平台上时,Pest-YOLO实现了36.2 FPS的实时推理,验证了其在资源受限环境中的实际适用性。本研究为粮食仓储智能化管理和害虫准确识别提供了切实可行的解决方案。
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引用次数: 0
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Journal of Stored Products Research
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