Pub Date : 2021-05-01DOI: 10.52228/jrub.2021-34-1-10
Deepali Nagre, R. Xalxo, V. Chandrakar, S. Keshavkant
The ability of melatonin to regulate number of physiological and biochemical processes under different environmental stresses has been widely studied in plants. So, this investigation was done to study the protective roles of melatonin on Cicer arietinum L. grown under arsenic stress. Subjecting Cicer arietinum L. seeds to arsenic stress caused significant decreases in germination percentage, radicle growth, biomass accumulation, protein content and activities of antioxidant enzymes. On the other hand, melatonin treatment significantly increased growth parameters and protein quantity via improving antioxidant enzyme systems as compared with their corresponding untreated controls.
{"title":"Impact of Melatonin on Growth and Antioxidant Activity of Cicer arietinum L. Grown under Arsenic Stress","authors":"Deepali Nagre, R. Xalxo, V. Chandrakar, S. Keshavkant","doi":"10.52228/jrub.2021-34-1-10","DOIUrl":"https://doi.org/10.52228/jrub.2021-34-1-10","url":null,"abstract":"\u0000 The ability of melatonin to regulate number of physiological and biochemical processes under different environmental stresses has been widely studied in plants. So, this investigation was done to study the protective roles of melatonin on Cicer arietinum L. grown under arsenic stress. Subjecting Cicer arietinum L. seeds to arsenic stress caused significant decreases in germination percentage, radicle growth, biomass accumulation, protein content and activities of antioxidant enzymes. On the other hand, melatonin treatment significantly increased growth parameters and protein quantity via improving antioxidant enzyme systems as compared with their corresponding untreated controls.\u0000","PeriodicalId":17214,"journal":{"name":"Journal of Ravishankar University (PART-B)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90839323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-05-01DOI: 10.52228/jrub.2021-34-1-9
S. Jain, S. Dewangan
The continuous rising abstraction resolution of distant police work sensors sets new interest for applications victimization this information. For mining valuable information from far flung police work data, various classifiers hooked in to the supernatural examination of individual pixels are projected and big advancement has been accomplished. Even so, these methodologies have their restrictions, for the foremost half they manufacture "salt and pepper" boisterous outcomes. to beat such problems, object-arranged image examination strategy hooked in to multi-resolution division methodology was advanced and it's been used for various application functions effectively. During this examination, a productive remotely detected image smart understanding technique hooked in to image division and geographical information framework (GIS) was projected, within the 1st place, division hooked in to mean shift was utilized to amass the underlying parts from distant police work footage. At that time, apply vectorization (Raster to Vector Convertor) strategy to supply polygons from the divided image and highlight attributions, as an example, ghostly, shape, surface then on square measure removed by zonal investigation hooked in to distinctive formation and polygons. At last, creating getting ready take a look at and administered characterization square measure dispensed. just about all means that square measure accomplished in geo-data framework with the exception of image division. supported the investigation, we have a tendency to engineered up a product arrangement of remotely detected image examination. Contrasted and also the understanding methodology of a business programming eCognition, the projected one was gettable and practiced once applied to the Quick bird remotely detected footage.
远程警务传感器的抽象分辨率不断提高，为利用这些信息的应用带来了新的兴趣。为了从广泛的警察工作数据中挖掘有价值的信息，各种分类器被投射到对单个像素的超自然检查中，并取得了很大的进展。即便如此，这些方法也有其局限性，对于前一半人来说，它们制造了“盐和胡椒”的喧闹结果。针对这一问题，提出了与多分辨率分割方法相结合的目标排列图像检测策略，并有效地应用于各种应用功能。在这次测试中，我们提出了一种高效的远程检测图像智能理解技术，该技术与图像分割和地理信息框架(GIS)相结合，首先，利用与均值移位相结合的分割来收集远程警察工作录像中的基础部分。然后，采用矢量化(Raster to Vector Convertor)策略，从分割后的图像中提取多边形并突出属性，例如，通过区域调查去除的鬼影、形状、表面和平方度量，钩住了独特的形状和多边形。最后，创建准备查看和管理表征方措施分配。除了图像分割之外，几乎所有的平方测量都是在地理数据框架中完成的。在调查的支持下，我们倾向于设计出一种远程检测图像检测的产品安排。对比了商业编程认知的理解方法，并将投影的认知方法应用于“快鸟”遥测录像中，得到了可理解和实践的结果。
{"title":"Remotely Sensed Image Based on Robust Segmentation and GIS System","authors":"S. Jain, S. Dewangan","doi":"10.52228/jrub.2021-34-1-9","DOIUrl":"https://doi.org/10.52228/jrub.2021-34-1-9","url":null,"abstract":"\u0000 The continuous rising abstraction resolution of distant police work sensors sets new interest for applications victimization this information. For mining valuable information from far flung police work data, various classifiers hooked in to the supernatural examination of individual pixels are projected and big advancement has been accomplished. Even so, these methodologies have their restrictions, for the foremost half they manufacture \"salt and pepper\" boisterous outcomes. to beat such problems, object-arranged image examination strategy hooked in to multi-resolution division methodology was advanced and it's been used for various application functions effectively. During this examination, a productive remotely detected image smart understanding technique hooked in to image division and geographical information framework (GIS) was projected, within the 1st place, division hooked in to mean shift was utilized to amass the underlying parts from distant police work footage. At that time, apply vectorization (Raster to Vector Convertor) strategy to supply polygons from the divided image and highlight attributions, as an example, ghostly, shape, surface then on square measure removed by zonal investigation hooked in to distinctive formation and polygons. At last, creating getting ready take a look at and administered characterization square measure dispensed. just about all means that square measure accomplished in geo-data framework with the exception of image division. supported the investigation, we have a tendency to engineered up a product arrangement of remotely detected image examination. Contrasted and also the understanding methodology of a business programming eCognition, the projected one was gettable and practiced once applied to the Quick bird remotely detected footage. \u0000","PeriodicalId":17214,"journal":{"name":"Journal of Ravishankar University (PART-B)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80255176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-09-23DOI: 10.52228/jrub.2019-32-1-11
Shrabani Karan, R. C. Agrawal
Investigations on ion-transport and materials properties of poly (ethylene oxide) (PEO) based Zn2+ conducting Nano-Composite Polymer Electrolyte (NCPE) membranes: [90 PEO: 10 Zn (CF3SO3)2] + xAl2O3, have been reported. NCPE films have been prepared by a completely dry hot-press cast technique using Solid Polymer Electrolyte (SPE) composition: [90 PEO: 10 Zn (CF3SO3)2] as I phase and Al2O3 nano-filler particles (< 50 nm) as II- Phase dispersoid. In an earlier study, SPE used here as I phase host has been identified as optimum room temperature conducting film exhibiting (srt) ~1.01 x 10-5 S/cm. As a consequence of fractional dispersal of nano-filler particles in SPE, additional srt enhancement of an order of magnitude was obtained. This has been referred as NCPE OCC film. Ion transport behavior in NCPE OCC has been characterized in terms of ionic conductivity (?), total ionic (tion)/cation (t+) transport numbers which have been measured using different ac/dc techniques. Temperature dependent conductivity study has also been carried out to understand the mechanism of ion transport and to compute activation energy (Ea) from ‘log s-1/T’ plot. Materials and thermal properties have been characterized with the help of SEM, XRD, FTIR and DSC / TGA techniques.
{"title":"Ion Transport and Materials Characterization Studies on Hot-Press Cast Zn2+ Conducting Nano-Composite Polymer Electrolyte (NCPE) Films: [90 PEO: 10 Zn (CF3SO3)2] + xAl2o3","authors":"Shrabani Karan, R. C. Agrawal","doi":"10.52228/jrub.2019-32-1-11","DOIUrl":"https://doi.org/10.52228/jrub.2019-32-1-11","url":null,"abstract":"\u0000 Investigations on ion-transport and materials properties of poly (ethylene oxide) (PEO) based Zn2+ conducting Nano-Composite Polymer Electrolyte (NCPE) membranes: [90 PEO: 10 Zn (CF3SO3)2] + xAl2O3, have been reported. NCPE films have been prepared by a completely dry hot-press cast technique using Solid Polymer Electrolyte (SPE) composition: [90 PEO: 10 Zn (CF3SO3)2] as I phase and Al2O3 nano-filler particles (< 50 nm) as II- Phase dispersoid. In an earlier study, SPE used here as I phase host has been identified as optimum room temperature conducting film exhibiting (srt) ~1.01 x 10-5 S/cm. As a consequence of fractional dispersal of nano-filler particles in SPE, additional srt enhancement of an order of magnitude was obtained. This has been referred as NCPE OCC film. Ion transport behavior in NCPE OCC has been characterized in terms of ionic conductivity (?), total ionic (tion)/cation (t+) transport numbers which have been measured using different ac/dc techniques. Temperature dependent conductivity study has also been carried out to understand the mechanism of ion transport and to compute activation energy (Ea) from ‘log s-1/T’ plot. Materials and thermal properties have been characterized with the help of SEM, XRD, FTIR and DSC / TGA techniques. \u0000","PeriodicalId":17214,"journal":{"name":"Journal of Ravishankar University (PART-B)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81009408","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-09-23DOI: 10.52228/jrub.2019-32-1-10
Rimsha Lakesh
The objective of the present empirical piece of research work is to examine the moderation effect of gender on the relationship between occupational aspiration and career maturity. Following the stratified random sampling technique 1000 students were drawn from different schools at Durg city, to serve as participants in the present research work. Career maturity was measured by Career Maturity Inventory (Gupta, 1989). Occupational aspiration was measured by Grewal (1975). Moderation effect was worked out through hierarchical multiple regression analysis. Result of the study indicated that, gender was significant moderator on the relationship between occupational aspiration and career maturity. It is concluded that there is sufficient empirical and statistical evidence of the moderation effect of gender on the relationship between relationship between occupational aspiration and career maturity.
{"title":"Moderating effect of gender on the association between occupational Aspiration and carrier maturity","authors":"Rimsha Lakesh","doi":"10.52228/jrub.2019-32-1-10","DOIUrl":"https://doi.org/10.52228/jrub.2019-32-1-10","url":null,"abstract":"\u0000 The objective of the present empirical piece of research work is to examine the moderation effect of gender on the relationship between occupational aspiration and career maturity. Following the stratified random sampling technique 1000 students were drawn from different schools at Durg city, to serve as participants in the present research work. Career maturity was measured by Career Maturity Inventory (Gupta, 1989). Occupational aspiration was measured by Grewal (1975). Moderation effect was worked out through hierarchical multiple regression analysis. Result of the study indicated that, gender was significant moderator on the relationship between occupational aspiration and career maturity. It is concluded that there is sufficient empirical and statistical evidence of the moderation effect of gender on the relationship between relationship between occupational aspiration and career maturity. \u0000","PeriodicalId":17214,"journal":{"name":"Journal of Ravishankar University (PART-B)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85598602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-09-23DOI: 10.52228/jrub.2019-32-1-12
Swati Sahu
An electrical model of dye-sensitized solar cell (DSSC) is derived on continuity and transport equations for all the four charged species i.e. electrons, iodide ions (I-), triiodide ions (I3-) and cations. The device model comprises of a pseudo-homogeneous active layer, where solar photovoltaic effect including both diffusion of electrons in nanoporous TiO2 layer as well as ions in electrolyte occur, and a bulk electrolyte layer, where only ions diffuse take place. The distribution of the electrons, iodide and tri-iodide ions as function of the pseudo-homogeneous active layer thickness of the DSSC under both the open-circuit and short-circuit operation conditions were performed. Parametric studies were conducted to analyze (J–V) characteristic of the DSSC with three different sets of porosity and also for different sets of TiO2 layer thicknesses.
{"title":"Electrical Modeling of Dye-Sensitized Solar Cells for Improving the Overall Photoelectric Conversion Efficiency","authors":"Swati Sahu","doi":"10.52228/jrub.2019-32-1-12","DOIUrl":"https://doi.org/10.52228/jrub.2019-32-1-12","url":null,"abstract":"\u0000 An electrical model of dye-sensitized solar cell (DSSC) is derived on continuity and transport equations for all the four charged species i.e. electrons, iodide ions (I-), triiodide ions (I3-) and cations. The device model comprises of a pseudo-homogeneous active layer, where solar photovoltaic effect including both diffusion of electrons in nanoporous TiO2 layer as well as ions in electrolyte occur, and a bulk electrolyte layer, where only ions diffuse take place. The distribution of the electrons, iodide and tri-iodide ions as function of the pseudo-homogeneous active layer thickness of the DSSC under both the open-circuit and short-circuit operation conditions were performed. Parametric studies were conducted to analyze (J–V) characteristic of the DSSC with three different sets of porosity and also for different sets of TiO2 layer thicknesses. \u0000","PeriodicalId":17214,"journal":{"name":"Journal of Ravishankar University (PART-B)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83333118","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-09-23DOI: 10.52228/jrub.2019-32-1-2
Digeshwar Prasad Sahu
Water is crucial and it has always been considered throughout history as a natural resource for the survival of humanity and other living beings therefore globally the shortage of quality water and its availability is an important issue. Groundwater is the only source of water of the Swami Vivekananda Airport Raipur, and it faces water crisis when the groundwater level decreases. In this scenario, Rain Water Harvesting (RWH) can contribute considerably to tackle these problems. Rainwater treatment systems can be installed at different potential locations in study area based on their physical site conditions such as slope, elevation etc. The airport has 38955.62 Square meters rooftop area out of 2.108 Square Kilometres total area, water collected from the rooftop will fulfil approximately 30% of the total daily demand of the airport. Apart from this airport is properly designed drainage system storm water is collected and separated into three potential zones. Zone I, II and III having area 0.2393, 0.4307 and 1.4382 sq. Kms respectively, which produce a different runoff amount based on maximum daily rainfall. Storm water is treated by Slow Sand Filter (SSF) of rate of 200 litres per hour per square meter and stored under design capacity, size and number of treatment unit depend on the maximum discharge at their respective zones. Treated rainwater is utilized for recharge purpose through the recharge trench when excess water comes from the rainwater tank. The cost-benefit analysis also done for before and after implementation of the project.
{"title":"Efficient Storm Water Management and Rainwater Harvesting System: a Case Study in Swami Vivekananda Airport Raipur","authors":"Digeshwar Prasad Sahu","doi":"10.52228/jrub.2019-32-1-2","DOIUrl":"https://doi.org/10.52228/jrub.2019-32-1-2","url":null,"abstract":"\u0000 Water is crucial and it has always been considered throughout history as a natural resource for the survival of humanity and other living beings therefore globally the shortage of quality water and its availability is an important issue. Groundwater is the only source of water of the Swami Vivekananda Airport Raipur, and it faces water crisis when the groundwater level decreases. In this scenario, Rain Water Harvesting (RWH) can contribute considerably to tackle these problems. Rainwater treatment systems can be installed at different potential locations in study area based on their physical site conditions such as slope, elevation etc. The airport has 38955.62 Square meters rooftop area out of 2.108 Square Kilometres total area, water collected from the rooftop will fulfil approximately 30% of the total daily demand of the airport. Apart from this airport is properly designed drainage system storm water is collected and separated into three potential zones. Zone I, II and III having area 0.2393, 0.4307 and 1.4382 sq. Kms respectively, which produce a different runoff amount based on maximum daily rainfall. Storm water is treated by Slow Sand Filter (SSF) of rate of 200 litres per hour per square meter and stored under design capacity, size and number of treatment unit depend on the maximum discharge at their respective zones. Treated rainwater is utilized for recharge purpose through the recharge trench when excess water comes from the rainwater tank. The cost-benefit analysis also done for before and after implementation of the project. \u0000","PeriodicalId":17214,"journal":{"name":"Journal of Ravishankar University (PART-B)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82488167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-09-23DOI: 10.52228/jrub.2019-32-1-6
Anshita Gupta
In India, Chhattisgarh is famous as Herbal state with its beautiful natural resources of forests and agricultural fields and value addition has been done so far in Chhattisgarh to maintain or improve the quality of the plants before the material reaches the industry. Presently, the herbal industry in Chhattisgarh is not very organized. The plant derived gums, mucilages from natural sources like carrageen an, thaumatin, lard, storax, agar, gum acacia, tragacanth and many more to name comply with many requirements of pharmaceutical excipients. These can be preferred for formulation development as being stable and involving less regulatory issues as compared to their synthetic counter parts. They can also be easily modified to meet the specific needs, thereby being a potent and economic vehicle for delivering active pharmaceutical ingredient in formulation. Thus our concerned and aims of the study is to throw light on the potential of natural excipients which can be proposed to be used as diluent, binder, disintegrant as well as lubricant in various types of formulations as they are biocompatible and capable of giving additional nutrition to the developed dosage form from indigeneous sources of Chhattisgarh state which could serve for cost effective and economical medicament and will enhance the value addition to the proposed herb and herbal species.
{"title":"Isolation, Characterization, Evaluation and Establishment of Polymeric Principles from Curcuma Angustifolia as Multitasking Excipient","authors":"Anshita Gupta","doi":"10.52228/jrub.2019-32-1-6","DOIUrl":"https://doi.org/10.52228/jrub.2019-32-1-6","url":null,"abstract":"\u0000 In India, Chhattisgarh is famous as Herbal state with its beautiful natural resources of forests and agricultural fields and value addition has been done so far in Chhattisgarh to maintain or improve the quality of the plants before the material reaches the industry. Presently, the herbal industry in Chhattisgarh is not very organized. The plant derived gums, mucilages from natural sources like carrageen an, thaumatin, lard, storax, agar, gum acacia, tragacanth and many more to name comply with many requirements of pharmaceutical excipients. These can be preferred for formulation development as being stable and involving less regulatory issues as compared to their synthetic counter parts. They can also be easily modified to meet the specific needs, thereby being a potent and economic vehicle for delivering active pharmaceutical ingredient in formulation. Thus our concerned and aims of the study is to throw light on the potential of natural excipients which can be proposed to be used as diluent, binder, disintegrant as well as lubricant in various types of formulations as they are biocompatible and capable of giving additional nutrition to the developed dosage form from indigeneous sources of Chhattisgarh state which could serve for cost effective and economical medicament and will enhance the value addition to the proposed herb and herbal species. \u0000","PeriodicalId":17214,"journal":{"name":"Journal of Ravishankar University (PART-B)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88079704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-09-23DOI: 10.52228/jrub.2019-32-1-9
R. Upadhyay
Nitrogen (N) is one of the most critical inputs and the current average nitrogen use efficiency (NUE) in the rice field is approximately 33%, poorest among cereals. Predominant form of N in aerobic soils is nitrate (NO3-) while ammonium (NH4+) exists in anaerobic soils. Development of cultivars with improved NH4+ or NO3- use efficiency by harnessing inherent significant variability for NUE can be an important approach. Considering these facts, the present study was established with one hundred twenty two and selected thirty two recombinant inbred lines (RILs) of two indica genotypes, Danteshwari × Dagad deshi under three nitrogen forms and three environments. The trend analysis of NH4+-N and NO3--N dynamics revealed that NH4+-N concentration persisted more under anaerobic condition and NO3--N concentration under aerobic conditions. Three way-ANOVA showed high level of significance for variance components (G, N, E) and their interactions effects (GXN, GXE, NXE, EXNXG) for yield and NUE and their component traits. Mean performance of genotypes depicted higher values for agronomically important traits i.e. yield and NUE under NH4+ as compared to NO3--N and N0. The phenotypic and genotypic data was statistically analyzed for QTLs identification for yield and NUE traits. A total of 58 QTLs conferring the corresponding five traits were detected under three N forms and two environments. We also investigated the different members of AMT (Ammonium transporters), NRT (Nitrate transporters), GS (Glutamine Synthetase) and GOGAT (Glutamate Synthase) genes, involved in NUE and analyzed the expression pattern of each gene using gene-specific primer in young rice seedlings. Collectively, OsGln1;1, OsGln1;2, OsGln1;3, OsGln2, OsGlt1 and OsGlt2 manifested different and reciprocal responses to nitrate and ammonium supply. The activity of enzymes NR, NiR, GS and GOGAT was significantly affected by NH4+and NO3- treatment. These results assist us to identify NH4+ and NO3- responsive cultivars which could be used for cultivation and/or used as parent’s in future breeding program to produce better nitrogen use efficiency varieties under water stress and non-stress conditions.
{"title":"Identification of QTLS for NH4+ and NO3- use efficiency under water stress and non-stress conditions and expression analysis of glutamine synthetase and nitrate reductase in rice (Oryza Sativa L.)","authors":"R. Upadhyay","doi":"10.52228/jrub.2019-32-1-9","DOIUrl":"https://doi.org/10.52228/jrub.2019-32-1-9","url":null,"abstract":"\u0000 Nitrogen (N) is one of the most critical inputs and the current average nitrogen use efficiency (NUE) in the rice field is approximately 33%, poorest among cereals. Predominant form of N in aerobic soils is nitrate (NO3-) while ammonium (NH4+) exists in anaerobic soils. Development of cultivars with improved NH4+ or NO3- use efficiency by harnessing inherent significant variability for NUE can be an important approach. Considering these facts, the present study was established with one hundred twenty two and selected thirty two recombinant inbred lines (RILs) of two indica genotypes, Danteshwari × Dagad deshi under three nitrogen forms and three environments. The trend analysis of NH4+-N and NO3--N dynamics revealed that NH4+-N concentration persisted more under anaerobic condition and NO3--N concentration under aerobic conditions. Three way-ANOVA showed high level of significance for variance components (G, N, E) and their interactions effects (GXN, GXE, NXE, EXNXG) for yield and NUE and their component traits. Mean performance of genotypes depicted higher values for agronomically important traits i.e. yield and NUE under NH4+ as compared to NO3--N and N0. The phenotypic and genotypic data was statistically analyzed for QTLs identification for yield and NUE traits. A total of 58 QTLs conferring the corresponding five traits were detected under three N forms and two environments. We also investigated the different members of AMT (Ammonium transporters), NRT (Nitrate transporters), GS (Glutamine Synthetase) and GOGAT (Glutamate Synthase) genes, involved in NUE and analyzed the expression pattern of each gene using gene-specific primer in young rice seedlings. Collectively, OsGln1;1, OsGln1;2, OsGln1;3, OsGln2, OsGlt1 and OsGlt2 manifested different and reciprocal responses to nitrate and ammonium supply. The activity of enzymes NR, NiR, GS and GOGAT was significantly affected by NH4+and NO3- treatment. These results assist us to identify NH4+ and NO3- responsive cultivars which could be used for cultivation and/or used as parent’s in future breeding program to produce better nitrogen use efficiency varieties under water stress and non-stress conditions. \u0000","PeriodicalId":17214,"journal":{"name":"Journal of Ravishankar University (PART-B)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90850480","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-09-23DOI: 10.52228/jrub.2019-32-1-5
Shraddha K. Chauhan, L. Upadhyay
Ground water arsenic contamination is one of the major global issues in recent days. The long exposure of arsenic can cause hazardous health effects like skin cancer, lung cancer as well as heart diseases. Conventional methods for monitoring arsenic level is often strenuous and require highly skilled personals to perform the measurements, thus there is a need to develop a simple and cost- effective techniques for quick and continuous monitoring. Paper-based approach for detecting the arsenic is a low-cost, non-instrumental method and provides on-site detection of water contaminants. In this study a rapid, simple and affordable method for arsenic detection was developed. This methodology involves the generation of arsine gas inside the analysis system that reacts with the silver nitrate present on the paper analytical device (PAD). This reaction between silver nitrate and arsine gives a colored product that was detected by scanning the colored intensity with image processing software. The iron oxide nanoparticles act as a reducing agent that causes the generation of arsine gas from arsenic. These nanoparticles were synthesized through green technology by using henna extract. The whole reaction takes place in the glass vial with the rubber cap on it at which the PAD was placed. Experimental parameters like concentration of reducing agent, silver nitrate and reaction time were evaluated that affects the performance of the analytical process. Under optimal conditions the method showed the detection limit of 0.005ppm.
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Pub Date : 2019-09-21DOI: 10.52228/jrub.2018-31-1-5
A. Sahu, Shraddha Pandey, Vindhya Patel, R. Khatoon, M. Nirmal, Kalpana Wani, D. Sahu, J. Goswami, C. Bhatt, Geetanjali Deshlahare, H. Sharma, Manish Kumar Raia, Joyce Rai
A new spectrophotometric method has been developed for determination of fungicide myclobutanil is based on the bromination of myclobutanil to form dibromo myclobutanil which react with Potassium iodide-Potassium iodate mixture in the presence of leucocrystal voilet (LCV) to form a violet colored complex. The complex shows maximum absorbance at 590 nm. Beer’s law obeyed over the concentration range of 0.5-4.0 µg in final solution volume of 10 mL. The reproducibility assessed by carrying out seven days replicate analysis of a solution containing 10 µg of myclobutanil in a final solution of 10 mL. The molar absorptivity of the coloured system is 1.29×105 L mol-1cm-1 and Sandell’s sensitivity is 1.03×10-3 µg cm-2. The standard deviation and relative standard deviation for the absorbance value were found to be ±0.00652 and 1.14% respectively. The proposed method is free from the interference of other toxicants. The analytical parameters were optimized and the method was applied to the determination of myclobutanil in water, soil and food samples.
用分光光度法测定杀菌剂菌丁腈的方法是将菌丁腈溴化生成二溴型菌丁腈，二溴型菌丁腈与碘化钾-碘酸钾混合物在白结晶紫(LCV)存在下反应生成紫色络合物。该配合物在590 nm处显示出最大吸光度。在最终溶液体积为10ml的0.5-4.0µg浓度范围内，遵循Beer定律。通过对含有10µg丁腈的溶液在10ml的最终溶液中进行7天的重复分析来评估再现性。着色体系的摩尔吸光度为1.29×105 L mol- ccm -1，桑德尔灵敏度为1.03×10-3µg cm-2。吸光度值的标准偏差为±0.00652，相对标准差为1.14%。所提出的方法不受其他毒物的干扰。优化了分析参数，并将该方法应用于水、土壤和食品样品中菌丁腈的测定。
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