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Inhibition of Biofilm Formation in Yersinia enterocolitica by Edible Plant Extracts Including Polygoni Multiflori Radix 何首乌等可食植物提取物对小肠结肠炎耶尔森菌生物膜形成的抑制作用
Q2 Engineering Pub Date : 2022-11-01 DOI: 10.5658/wood.2022.50.6.448
Youngseok Ham, Tae-Jong Kim
Yersinia enterocolitica , which causes yersiniosis, is a bacterium that produces biofilms effectively. The inhibition of biofilm formation provides a method for preventing infections with Y. enterocolitica . In this study, the inhibitory activity of Y. enterocolitica biofilm formation was investigated in a library of 140 edible plant methanol extracts including forest products. It was identified that the biofilm formation could be inhibited by 12 extracts of plants, Agastachis Herba, Agrimoniae Herba, Diospyros kaki leaves, Elsholtziae Herba, Ginkgonis Semen, Lycopi Herba, Melonis Pedicellus, Menthae Herba, Mori Radicis Cortex, Polygoni Multiflori Radix, Prunellae Spica, and Schizonepetae Spica. After changing the solvent to ethanol and water, the greatest inhibition of biofilm formation was produced by a 50% ethanol extract of Polygoni Multiflori Radix. A method to effectively prevent yersiniosis can be developed using the edible plant extracts identified in this study.
小肠结肠炎耶尔森菌是一种能有效产生生物膜的细菌,可引起耶尔森菌病。对生物膜形成的抑制提供了一种预防小肠结肠炎杆菌感染的方法。在本研究中,在含有140种可食用植物甲醇提取物(包括林产品)的文库中研究了小肠结肠炎Y菌生物膜形成的抑制活性。经鉴定,Agastachis Herba,Agrimoniae Herba,Diospyross kaki leaves,Elsholtzia Herba,Ginkgonis Semen,Lycopi Herba,Melonis Pedicellus,Mentae Herba、Mori Radicis Cortex,Polygoni Multiflori Radix,Prunellae Spica和Schizonepetae Spica等12种植物提取物均可抑制生物膜的形成。将溶剂改为乙醇和水后,何首乌50%乙醇提取物对生物膜形成的抑制作用最大。利用本研究中鉴定的可食用植物提取物,可以开发出一种有效预防耶尔森病的方法。
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引用次数: 0
Mask Region-Based Convolutional Neural Network (R-CNN) Based Image Segmentation of Rays in Softwoods 基于掩模区域的卷积神经网络(R-CNN)的软木光线图像分割
Q2 Engineering Pub Date : 2022-11-01 DOI: 10.5658/wood.2022.50.6.490
Hye-Ji Yoo, O. Kwon, Jeong-Wook Seo
The current study aimed to verify the image segmentation ability of rays in tangential thin sections of conifers using artificial intelligence technology. The applied model was Mask region-based convolutional neural network (Mask R-CNN) and softwoods (viz. Picea jezoensis , Larix gmelinii , Abies nephrolepis , Abies koreana , Ginkgo biloba , Taxus cuspidata , Cryptomeria japonica , Cedrus deodara , Pinus koraiensis ) were selected for the study. To take digital pictures, thin sections of thickness 10–15 μm were cut using a microtome, and then stained using a 1:1 mixture of 0.5% astra blue and 1% safranin. In the digital images, rays were selected as detection objects, and Computer Vision Annotation Tool was used to annotate the rays in the training images taken from the tangential sections of the woods. The performance of the Mask R-CNN applied to select rays was as high as 0.837 mean average precision and saving the time more than half of that required for Ground Truth. During the image analysis process, however, division of the rays into two or more rays occurred. This caused some errors in the measurement of the ray height. To improve the image processing algorithms, further work on combining the fragments of a ray into one ray segment, and increasing the precision of the boundary between rays and the neighboring tissues is required.
本研究旨在利用人工智能技术验证光线在针叶树切向薄片中的图像分割能力。应用的模型是基于Mask区域的卷积神经网络(Mask R-CNN),并选择了一些软木(即日本云杉、落叶松、冷杉、红松、银杏、红豆杉、日本柳杉、雪松、红松)进行研究。为了拍摄数字照片,使用切片机切割厚度为10-15μm的薄片,然后使用0.5%阿斯特拉蓝和1%藏红的1:1混合物进行染色。在数字图像中,选择射线作为检测对象,并使用计算机视觉注释工具对从木材切向截面拍摄的训练图像中的射线进行注释。应用于选择射线的Mask R-CNN的性能高达0.837的平均精度,并且节省了超过Ground Truth所需时间的一半的时间。然而,在图像分析过程中,出现了将射线分割为两条或更多条射线的情况。这在射线高度的测量中造成了一些误差。为了改进图像处理算法,需要进一步将射线片段组合成一个射线片段,并提高射线与相邻组织之间边界的精度。
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引用次数: 0
Conversion of Glucose and Xylose to 5-Hydroxymethyl furfural, Furfural, and Levulinic Acid Using Ethanol Organosolv Pretreatment under Various Conditions 不同条件下乙醇-有机溶剂预处理葡萄糖和木糖制备5-羟甲基糠醛、糠醛和乙酰丙酸
Q2 Engineering Pub Date : 2022-11-01 DOI: 10.5658/wood.2022.50.6.475
K. Gwak, Chae-Hwi Yoon, Jong-Chan Kim, Jong-Hwa Kim, Young-Min Cho, I. Choi
The objective of this study was to understand the conversion characteristics of glucose and xylose using the major monosaccharide standards for lignocellulosic biomass. The acid-catalyzed organosolv pretreatment conducted using ethanol was significantly different from the acid-catalyzed process conducted in an aqueous medium. 5-hydroxymethylfurfural (5-HMF), levulinic acid and furfural were produced from glucose conversion. The maximum yield of 5-HMF was 5.5%, at 200℃, when 0.5% sulfuric acid was used. The maximum yield of levulinic acid was 21.5%, at 220℃, when 1.0% sulfuric acid was used. Furfural was produced from xylose conversion and under 0.5% sulfuric acid, furfural reached the maximum yield 48.5% at 210℃. Ethyl levulinate and methyl levulinate were also formed from the glucose standard following the esterification reaction conducted under conditions of the combined conversion method, which proceeded under both ethanol-rich and water-rich conditions.
本研究的目的是使用木质纤维素生物质的主要单糖标准来了解葡萄糖和木糖的转化特性。使用乙醇进行的酸催化有机溶剂预处理与在水性介质中进行的酸催化剂工艺显著不同。葡萄糖转化生成5-羟甲基糠醛(5-HMF)、乙酰丙酸和糠醛。当硫酸用量为0.5%时,在200℃时,5-HMF的最大产率为5.5%。在220℃,使用1.0%硫酸时,乙酰丙酸的最大产率为21.5%。木糖转化制备糠醛,在0.5%硫酸作用下,糠醛在210℃时的最高产率为48.5%。在富乙醇和富水条件下进行的组合转化法条件下进行酯化反应后,乙酰丙酸乙酯和乙酰丙酸甲酯也由葡萄糖标准物形成。
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引用次数: 0
Evaluation of Anti-Asthmatic Activity of Essential Oils from the Lauraceae Family in Lipopolysaccharide (LPS)-Stimulated NCI-H292 Cells 樟科精油对脂多糖(LPS)刺激NCI-H292细胞抗哮喘活性的评价
Q2 Engineering Pub Date : 2022-11-01 DOI: 10.5658/wood.2022.50.6.414
Jiyoon Yang, Su-Yeon Lee, Hyunjeong Na, Soo-Kyeong Jang, M. Park
The Lauraceae family has commercial uses, such as in the food, pharmaceutical
樟科具有商业用途,如食品、医药
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引用次数: 1
Mechanical Properties of Cork Composite Boards Reinforced with Metal, Glass Fiber, and Carbon Fiber 金属、玻璃纤维和碳纤维增强软木复合板的机械性能
Q2 Engineering Pub Date : 2022-11-01 DOI: 10.5658/wood.2022.50.6.427
Min-Seong Cha, So-Jeong Yoon, Jin-Ho Kwon, H. Byeon, Han-Min Park
For effective applicability of reinforced cork, cork composites reinforced with metal, glass fiber, and carbon fiber were developed, and the effects of the reinforcing materials on the mechanical properties of cork composites were investigated. The bending moduli of elasticity (MOE) of cork composites were in the 32.7–35.9 MPa range, while the bending strength values were in the 1.62–1.73 MPa range. The strength performance decreased in the order cork-metal > cork-carbon fiber > cork-glass fiber. The bending MOEs were improved by 29%–41% compared with simple cork boards, while the bending strengths of reinforced cork were 35%–45% higher. The strength performance significantly improved following the incorporation of thin mesh materials into the middle layer of the studied cork composites. The bending strains of the cork composites were remarkably higher compared with oak wood, making them promising for applications that require bending processing, such as curved jointing. The internal bond strengths of the cork composites were 0.26–0.44 MPa, approximately 0.36–0.60 times lower compared with medium-density fiber boards.
为了增强软木的有效适用性,开发了金属、玻璃纤维和碳纤维增强软木复合材料,并研究了增强材料对软木复合材料力学性能的影响。软木复合材料的弯曲弹性模量(MOE)在32.7–35.9 MPa范围内,而弯曲强度值在1.62–1.73 MPa范围内。强度性能下降的顺序为软木金属>软木碳纤维>软木玻璃纤维。与简单软木板相比,弯曲弹性模量提高了29%-41%,而增强软木的弯曲强度提高了35%-45%。在所研究的软木复合材料的中间层中加入薄网格材料后,强度性能显著提高。与橡木相比,软木复合材料的弯曲应变明显更高,这使其有望用于需要弯曲加工的应用,如弯曲连接。软木复合材料的内部粘结强度为0.26–0.44 MPa,与中密度纤维板相比约低0.36–0.60倍。
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引用次数: 3
Synchrotron X-Ray Diffraction Studies on Crystalline Domains in Urea–Formaldehyde Resins at Low Molar Ratio 低摩尔比脲醛树脂晶体结构的同步加速器x射线衍射研究
Q2 Engineering Pub Date : 2022-09-01 DOI: 10.5658/wood.2022.50.5.353
E. Wibowo, Byung‐Dae Park, V. Causin, Dongyup Hahn
The crystalline domain of thermosetting urea–formaldehyde (UF) resins at low formaldehyde-to-urea (F/U) molar ratios (≤ 1.0) is known to be responsible for their poor performance as wood adhesives. Crystallization has been observed in 1.0 F/U UF resins during the addition reaction stage and at the end of the synthesis process (neat UF resins). The crystallinity and X-ray diffraction (XRD) spectra of the uncured neat UF resins, on the other hand, differed significantly from those of the cured neat UF resins, raising the possibility that their crystal structures were also different. This study demonstrates for the first time that the crystalline domains in 1.0 F/U UF resins generated from uncured and cured samples are identical. Despite having a lower crystallinity value, the synchrotron XRD patterns of purified neat UF resins were equivalent to the XRD patterns of cured neat UF resins. Transmission electron microscope images of the cured UF resins showed that the crystals were lamellar structures. This finding suggests that the crystal at low molar ratio UF resins are isotropic polycrystals with random orientation.
众所周知,在低甲醛与尿素(F/U)摩尔比(≤1.0)下,热固性脲醛(UF)树脂的结晶域是其作为木材粘合剂性能较差的原因。在添加反应阶段和合成过程结束时,在1.0F/U的UF树脂中观察到结晶(纯UF树脂)。另一方面,未固化的纯UF树脂的结晶度和X射线衍射(XRD)光谱与固化的纯脲醛树脂的结晶性和X射线XRD光谱显著不同,这增加了它们的晶体结构也不同的可能性。本研究首次证明,由未固化和固化样品产生的1.0F/U UF树脂中的结晶畴是相同的。尽管具有较低的结晶度值,但纯化的纯UF树脂的同步加速器XRD图谱与固化的纯脲醛树脂的XRD图谱相当。固化后的UF树脂的透射电镜图像显示,其晶体为层状结构。这一发现表明,低摩尔比UF树脂的晶体是具有随机取向的各向同性多晶体。
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引用次数: 0
Monitoring of Moisture and Dimensional Behaviors of Nail-Laminated Timber (NLT)-Concrete Slab Exposed to Outdoor Air 暴露在室外空气中的钉层合木材(NLT)-混凝土板的水分和尺寸特性监测
Q2 Engineering Pub Date : 2022-09-01 DOI: 10.5658/wood.2022.50.5.301
Sung-Wook Hwang, Hyunwoo Chung, Taekyeong Lee, Kyung-Sun Ahn, S. Pang, Jun-Sun Bang, H. Kwak, Jung-Kwon Oh, H. Yeo
The moisture and dimensional behaviors of a nail-laminated timber (NLT)–concrete slab composed of an NLT–plywood composite and topping concrete are monitored for 385 days. The slab is developed for using as flexural elements such as floors. The humidity control of wood gently introduces significant fluctuations under the ambient relative humidity into the slab, and fluctuations in the relative humidity result in dimensional changes. The equilibrium moisture content of the slab increases from 6.7% to 15.3% during the monitoring period, resulting in a width (radial) strain of 0.58%. The length (longitudinal) strain is negligible, and the height (tangential) strain is excluded from the analysis because of abstruse signal patterns generated. Concrete pouring causes a permanent increase in the width of the NLT–plywood composite. However, the width deforms because the weight of the concrete mixture loosens the nail-laminated structure, not because of the significant amount of moisture in the mixture. The dimensional stabilization effect of the nail-laminated system is demonstrated as the composite strain is lower than the total strain of lumber and plywood, which are elements constituting the nail-laminated structure.
对由NLT胶合板复合材料和浇头混凝土组成的钉层木材混凝土板的水分和尺寸行为进行了385天的监测。该板是为使用弯曲元件如地板而开发的。木材的湿度控制轻轻地将环境相对湿度下的显著波动引入板材,相对湿度的波动导致尺寸变化。在监测期间,板坯的平衡含水率从6.7%增加到15.3%,导致宽度(径向)应变为0.58%。长度(纵向)应变可以忽略不计,高度(切向)应变由于产生的信号模式深奥而被排除在分析之外。混凝土浇筑导致nlt -胶合板复合材料的宽度永久增加。然而,宽度变形是因为混凝土混合物的重量使钉层压结构松动,而不是因为混合物中含有大量的水分。钉层合结构的复合应变小于构成钉层合结构的木材和胶合板的总应变,证明了钉层合结构的尺寸稳定效果。
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引用次数: 3
Investigation of Sound Absorption Ability of Hinoki Cypress (Chamaecyparis obtusa) Cubes 日本柏木(Chamaecyparis obtusa)立方体吸声性能的研究
Q2 Engineering Pub Date : 2022-09-01 DOI: 10.5658/wood.2022.50.5.365
E. Jang
an improvement in immune that helps ABSTRACT Today, commercialized Hinoki cypress cubes are used for fragrance, humidification, and pillows in Korea. In this study, the sound absorption ability of Hinoki cypress ( Chamaecyparis obtusa ) cubes was examined. The three groups of Hinoki cypress cubes were prepared depending on their dimension (L: 9 × 9 × 9, M: 7 × 7 × 7, S: 4 × 4 × 4 mm). Their sound absorption coefficient was examined after filling 6, 8, 10, and 12 cm height in impedance tubes, respectively. Overall, the sound absorption ability depending on dimension was superior in the M group compared to the L and S groups. Also, as the filling height increased, the sound absorption capacity increased. In sum, noise reduction coefficients (NRC) of all Hinoki cypress cubes were 0.41–0.59. Thus, this research found that Hinoki cypress cubes have a sound-absorbing function.
摘要:如今,在韩国,商业化的日红柏木方块被用于香料、保湿剂和枕头。本研究考察了日本柏木(Chamaecyparis obtusa)立方体的吸声能力。根据尺寸大小(L: 9 × 9 × 9, M: 7 × 7 × 7, S: 4 × 4 × 4 mm)制备了3组扁柏立方体。分别在阻抗管中填充6,8,10,12 cm高度后检测其吸声系数。总体而言,M组对尺寸的吸声能力优于L组和S组。随着填充高度的增加,吸声能力也随之增加。结果表明,各树种的降噪系数(NRC)均在0.41 ~ 0.59之间。因此,本研究发现,日木柏木立方体具有吸声功能。
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引用次数: 5
Antioxidative and Circadian Rhythm Regulation Effect of Quercus gilva Extract 栎树提取物的抗氧化及昼夜节律调节作用
Q2 Engineering Pub Date : 2022-09-01 DOI: 10.5658/wood.2022.50.5.338
Jin-Sung Huh, Sora Lee, Dong-Soo Kim, M. Choi, Hyunmo Choi, Kyung-Ha Lee
Herein, water and ethanol extracts were obtained from the leaves, branches, kernels, and pericarp of Quercus gilva and subsequently analyzed for antioxidant activity and circadian rhythm regulation effects. Candidate components that may affect circadian rhythm and antioxidant activity were investigated to discover potential functional materials. Antioxidant activity was analyzed via 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2’-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging activity assays, showing that the hot water extract exhibited higher activity than that of the ethanol extract. In particular, the branch extract showed high antioxidant activity. By measuring total contents of polyphenols, flavonoids, and tannins, the hot water branch extract showed the highest concentrations, highlighting their significant contribution to the antioxidant activity. Examination of the circadian rhythm regulation of each extract showed that the ethanol extract exhibited greater impacts on the circadian rhythm amplitude compared to the water extract. The branch ethanol extract induced circadian rhythm amplitude changes via clock gene Bmal1 expression regulation. Determination of 12 phenolic compound concentrations showed that the branch ethanol extract contained many phenolic compounds, including catechin. This suggests that these compounds affected circadian rhythm regulation. In conclusion, the hot water branch extract has potential as an natural antioxidant material, while the corresponding ethanol extract has potential as a functional material for regulating circadian rhythm.
本研究从栎树的叶、枝、仁和果皮中提取水和乙醇提取物,分析其抗氧化活性和昼夜节律调节作用。研究了可能影响昼夜节律和抗氧化活性的候选成分,以发现潜在的功能材料。通过2,2-二苯基-1-吡啶肼(DPPH)和2,2 ' -氮基-双(3-乙基苯并噻唑-6-磺酸)(ABTS)自由基清除实验分析了其抗氧化活性,结果表明热水提取物比乙醇提取物具有更高的抗氧化活性。其中,分枝提取物具有较高的抗氧化活性。通过对多酚类、黄酮类和单宁类总含量的测定,热水支提物的含量最高,显示出其对抗氧化活性的显著贡献。对每种提取物的昼夜节律调节的检查表明,与水提取物相比,乙醇提取物对昼夜节律幅度的影响更大。树枝乙醇提取物通过调节生物钟基因Bmal1的表达诱导昼夜节律振幅的变化。对12种酚类化合物的浓度测定表明,枝乙醇提取物中含有多种酚类化合物,其中包括儿茶素。这表明这些化合物影响昼夜节律调节。综上所述,热水分支提取物具有作为天然抗氧化物质的潜力,而相应的乙醇提取物具有作为调节昼夜节律功能物质的潜力。
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引用次数: 2
Influence of Alkali and Silane Treatment on the Physico-Mechanical Properties of Grewia serrulata Fibres 碱处理和硅烷处理对细齿Grewia纤维物理力学性能的影响
Q2 Engineering Pub Date : 2022-09-01 DOI: 10.5658/wood.2022.50.5.325
Bhupesh Jain, R. Mallya, S. Nayak, Srinivas Shenoy Heckadka, S. Prabhu, G. Mahesha, G. Sancheti
Grewia serrulata fibres were chemically treated with 3%, 6%, and 9% NaOH for the duration of 4 h. Additionally, the NaOH-treated fibres were also treated with 3 – (trimethoxysilyl) propyl methacrylate (silane). Properties such as density and tensile strength of the treated fibres were compared against the untreated fibres. The highest density was obtained in the case of 9% NaOH + silane treated fibres, which was 26.47% higher than untreated fibres, implying effective removal of hemicellulose. Likewise, the highest tensile strength was also obtained in the case of 9% NaOH + silane treated fibres. The increment observed in the tensile strength of the natural fibres was related to the removal of impurities, hemicellulose, and stress-raisers as well as deposition over the fibre surface that smoothed it. These observations were further validated by estimating changes in chemical constituents due to chemical treatment along with characterization techniques such as scanning electron microscopy and thermogravimetric analysis.
用3%、6%和9%的NaOH对Grewia serrulata纤维进行化学处理,持续4小时。此外,NaOH处理的纤维还用甲基丙烯酸3-(三甲氧基甲硅烷基)丙酯(硅烷)处理。将处理过的纤维的密度和拉伸强度等性能与未处理的纤维进行比较。在9%NaOH+硅烷处理的纤维的情况下获得了最高的密度,其比未处理的纤维高26.47%,这意味着半纤维素的有效去除。同样,在9%NaOH+硅烷处理的纤维的情况下也获得了最高的拉伸强度。观察到的天然纤维抗拉强度的增加与杂质、半纤维素和应力促进剂的去除以及在使其光滑的纤维表面上的沉积有关。通过估计化学处理引起的化学成分变化以及扫描电子显微镜和热重分析等表征技术,进一步验证了这些观察结果。
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引用次数: 3
期刊
Journal of the Korean wood science and technology
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