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Coicis Semen Reduces Staphylococcus aureus Persister Cell Formation by Increasing Membrane Permeability 薏苡精液通过增加膜渗透性减少金黄色葡萄球菌固着细胞的形成
Q2 Engineering Pub Date : 2024-03-01 DOI: 10.5658/wood.2024.52.2.145
Min-jun Kim, Tae-Jong Kim
Unlike resistant cells, persister cells resist antibiotics due to a decreased cellular metabolic rate and can transition back to normal susceptible cells when the antibiotic is removed. These persister cells contribute to the chronic symptoms of infectious diseases and promote the emergence of resistant strains with continuous antibiotic exposure. Therefore, eliminating persister cells represents a promising approach to significantly enhance antibiotic efficacy. Here, we found that Coicis Semen extract reduced Staphylococcus aureus persister cells at a concentration of 0.5 g/L. Linoleic acid and oleic acid, the major components of Coicis Semen extract, exhibited a comparable reduction in persister cells when combined with three antibiotics: ciprofloxacin, oxacillin, and tobramycin. Conversely, these effects were nullified in the presence of the surfactant Tween 80 (1%), suggesting that the hydrophobic characteristics of linoleic acid and oleic acids play a pivotal role in reducing the number of S. aureus persister cells. Considering the concentration-dependent effects of linoleic acid and oleic acid, the persister-reducing activity of Coicis Semen extract was primarily attributed to these fatty acids. Moreover, Coicis Semen extract, linoleic acid, and oleic acid increased the cell membrane permeability of S. aureus . Interestingly, this effect was counteracted by 1% Tween 80, indicating a close association between the reduction of persister cells and the increase in cell membrane permeability. The identified compounds could thus be used to eliminate persister cells, thereby enhancing therapeutic efficacy and shortening treatment duration. When used in conjunction with antibiotics, they may also mitigate chronic symptoms and significantly reduce the emergence of antibiotic-resistant bacteria.
与耐药细胞不同,顽固细胞由于细胞新陈代谢率降低而对抗生素产生抗药性,当抗生素被移除时,顽固细胞又会变回正常的易感细胞。这些持久细胞会导致感染性疾病的慢性症状,并在持续接触抗生素的情况下促进耐药菌株的出现。因此,消除顽固细胞是显著提高抗生素疗效的有效方法。在这里,我们发现薏苡仁提取物在 0.5 克/升的浓度下可减少金黄色葡萄球菌的固着细胞。薏苡精提取物的主要成分亚油酸和油酸与环丙沙星、氧沙西林和妥布霉素三种抗生素联合使用时,可减少金黄色葡萄球菌的固着细胞。相反,在表面活性剂吐温 80(1%)存在的情况下,这些效果被抵消,这表明亚油酸和油酸的疏水特性在减少金黄色葡萄球菌宿主细胞数量方面发挥了关键作用。考虑到亚油酸和油酸的作用与浓度有关,薏苡仁提取物的减少宿主活性主要归功于这两种脂肪酸。此外,薏苡仁提取物、亚油酸和油酸增加了金黄色葡萄球菌细胞膜的通透性。有趣的是,1%的吐温80可以抵消这种效应,这表明顽固细胞的减少与细胞膜通透性的增加密切相关。因此,所发现的化合物可用于消除顽固细胞,从而提高疗效并缩短治疗时间。与抗生素一起使用时,它们还可以减轻慢性症状,并显著减少耐抗生素细菌的出现。
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引用次数: 0
Anti-termite Activity of Tamanu Bark Extract (Calophyllum inophyllum L.) 塔玛努树皮提取物(Calophyllum inophyllum L.)的抗蚁活性
Q2 Engineering Pub Date : 2024-03-01 DOI: 10.5658/wood.2024.52.2.134
Ainun Zalsabila, W. Syafii, T. Priadi, Syahidah
This study aimed to analyze the anti-termite properties of tamanu ( Calophyllum inophyllum L.) stem bark extracts against subterranean termites, specifically, Coptotermes curvignathus . The bark powder of C. inophyllum was extracted using different solvents, such as n-hexane, ethyl acetate, and methanol, using the maceration method. Anti-termite testing was performed using two paper disc methods: no-and two-choice tests. Whatman test paper was dripped with the extract solutions at concentrations of 4%, 6%, 8%, and 10% (w/v). Subsequently, the treated paper disc was placed into an acrylic tube, and the subterranean termite was added. The parameters utilized in the test included termite mortality and the weight loss of the test paper. The results revealed that the total extract yield of C. inophyllum stem bark was 30.24%. Furthermore, the extractive substances from C. inophyllum bark exhibited anti-termite activity. The most favorable outcomes were obtained with the n-hexane and ethyl acetate extracts at a concentration of 10%. The termite mortality and weight loss of the test paper were respectively 66% and 5.67% for the n-hexane extract and 66.67% and 6.19% for the ethyl acetate extract. In addition, the n-hexane extract contained friedelan-3-one, while the ethyl acetate extract contained 1,2-benzene dicarboxylic acid, dinonyl ester, and friedelan-3-one. The results suggested that these compounds are responsible for the observed anti-termite activity.
本研究旨在分析茶树(Calophyllum inophyllum L.)茎皮提取物对地下白蚁(特别是Coptotermes curvignathus)的抗蚁特性。使用正己烷、乙酸乙酯和甲醇等不同溶剂,采用浸渍法提取茶树树皮粉末。抗白蚁试验采用两种纸盘法:无选择和双选择试验。在 Whatman 试纸上滴入浓度为 4%、6%、8% 和 10%(w/v)的提取物溶液。随后,将处理过的纸盘放入丙烯酸管中,并加入地下白蚁。试验中使用的参数包括白蚁死亡率和试纸的重量损失。结果显示,茶树茎皮的总萃取率为 30.24%。此外,从茶树树皮中提取的物质具有抗白蚁活性。浓度为 10%的正己烷和乙酸乙酯提取物的效果最好。正己烷提取物的白蚁死亡率和试纸重量损失率分别为 66% 和 5.67%,乙酸乙酯提取物的白蚁死亡率和试纸重量损失率分别为 66.67% 和 6.19%。此外,正己烷提取物中含有油炸鞣剂-3-酮,而乙酸乙酯提取物中含有 1,2-苯二羧酸、二壬酯和油炸鞣剂-3-酮。结果表明,这些化合物是产生所观察到的抗白蚁活性的原因。
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引用次数: 0
A Study on the Positioning Strategy of Wood Cultural Experience Center 伍德文化体验中心定位战略研究
Q2 Engineering Pub Date : 2024-03-01 DOI: 10.5658/wood.2024.52.2.175
Kyungrok Won, Jinwoong Byeon, Dowoong Yoon, Jonghye Park, Hanmin Park, Heeseop Byeon
The increase in atmospheric carbon dioxide concentrations is known to be closely associated with climate change and global warming. In this sense, considering that facilities for appropriate education and experience on wood, which is a carbon pool, have been required, this study targets the Wood Cultural Experience Centers, which are in current operation, examines and evaluates their operation status and policy changes
众所周知,大气中二氧化碳浓度的增加与气候变化和全球变暖密切相关。从这个意义上讲,考虑到需要建立适当的教育和体验设施,对作为碳库的木材进行教育和体验,本研究以目前正在运营的木材文化体验中心为对象,对其运营状况和政策变化进行研究和评估。
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引用次数: 0
Conversion Characteristics of Chemical Constituents in Liriodendron tulipifera and Their Influences on Biomass Recalcitrance during Acid-Catalyzed Organosolv Pretreatment 酸催化有机溶胶预处理过程中 Liriodendron tulipifera 中化学成分的转化特征及其对生物质再抗性的影响
Q2 Engineering Pub Date : 2024-03-01 DOI: 10.5658/wood.2024.52.2.101
K. Gwak, Jun-Ho Shin, Chae-Hwi Yoon, In-Gyu Choi
The conversion characteristics of the major components of Liriodendron tulipifera were investigated during acid-catalyzed organosolv pretreatment. Glucan in L. tulipifera was slowly hydrolyzed, whereas xylan was rapidly hydrolyzed. Simultaneous hydrolysis and degradation of xylan and lignin occurred; however, after complete hydrolysis of xylan at higher temperatures, lignin remained and was not completely degraded or solubilized. These conversion characteristics influence the structural properties of glucan in L. tulipifera . Critical hydrolysis of the crystalline regions in glucan occurred along with rapid hydrolysis of the amorphous regions in xylan and lignin. Breakdown of internal lignin and xylan bonds, along with solubilization of lignin, causes destruction of the lignin-carbohydrate complex. Over a temperature of 160℃, the lignin that remained was coalesced, migrated, and re-deposited on the surface of pretreated solid residue, resulting in a drastic increase in the number and content of lignin droplets. From the results, the characteristic conversions of each constituent and the changes in the structural properties in L. tulipifera effectively improved enzymatic hydrolysis in the range of 140℃–150℃. Therefore, it can be concluded that significant changes in the biomass recalcitrance of L. tulipifera occurred during organosolv pretreatment.
研究了在酸催化有机溶胶预处理过程中郁金香主要成分的转化特性。郁金香中的葡聚糖被缓慢水解,而木聚糖则被快速水解。木聚糖和木质素同时发生水解和降解;然而,木聚糖在较高温度下完全水解后,木质素仍然存在,没有完全降解或溶解。这些转化特征影响了 L. tulipifera 中葡聚糖的结构特性。在木聚糖和木质素的无定形区域快速水解的同时,葡聚糖的结晶区域也发生了临界水解。木质素和木聚糖内部键的断裂以及木质素的溶解导致了木质素-碳水化合物复合物的破坏。在 160℃的温度下,残留的木质素凝聚、迁移并重新沉积在预处理过的固体残渣表面,导致木质素液滴的数量和含量急剧增加。从结果来看,在 140℃-150℃ 范围内,郁金香中各成分的特征转化率和结构特性的变化有效地改善了酶水解作用。因此,可以得出结论:在有机溶胶预处理过程中,郁金香的生物质再钙化性发生了显著变化。
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引用次数: 0
Utilization of Sapwood Waste of Fast-Growing Teak in Activated Carbon Production and Its Adsorption Properties 利用速生柚木边材废料生产活性炭及其吸附性能
Q2 Engineering Pub Date : 2024-03-01 DOI: 10.5658/wood.2024.52.2.118
J. Sutapa, G. Lukmandaru, S. Sunarta, R. Pujiarti, D. Irawati, Rizki Arisandi, Riska Dwiyanna, Robertus Danu Priyambodo
The sapwood portion of fast-growing teak is mostly ignored due to its inferior quality. One of the possibilities for utilizing sapwood waste is to convert it into activated carbon that has good adsorption capabilities. The raw materials used in this research were sapwood of 14-year-old fast-growing teak sapwood (FTS) waste, which was taken from three trees from community forests in Wonosari, Gunungkidul, Yogyakarta Special Region. FTS waste was taken from the bottom of the tree up to a height of 1.3 m. The activation process is conducted with an activation temperature of 750℃, 850℃, and 950℃. The heating duration consists of three variations: 30 min, 60 min, and 90 min. The quality evaluation parameters of activated carbon include yield, moisture content, volatile matter content, ash content, fixed carbon content, adsorption capacity of benzene, adsorption capacity of methylene blue, and adsorption capacity of iodine. The results showed that the activated carbon produced had the following quality parameters: yield of 75.61%; moisture content of 1.27%; volatile matter content of 9.98%; ash content of 5.43%; fixed carbon content of 84.58%; benzene absorption capacity of 8.58%; methylene blue absorption capacity of 87.73 mg/g; and iodine adsorption capacity of 948.19 mg/g. It can be concluded that activated carbon from FTS waste has good iodine adsorption, which fulfilled the SNI 06-3730-1995 quality standard. Due to the iodine adsorption ability of FTS waste activated carbon, the conversion of FTS waste to activated carbon is categorized as a potential method to increase the value of this material.
速生柚木的边材部分由于质量较差,大多被忽视。利用边材废料的可能性之一是将其转化为具有良好吸附能力的活性炭。本研究使用的原材料是 14 年树龄的速生柚木边材(FTS)废料,取自日惹特区古农基杜市沃诺萨里社区森林的三棵树。活化过程的活化温度分别为 750℃、850℃ 和 950℃。加热时间有三种变化:30 分钟、60 分钟和 90 分钟。活性炭的质量评价参数包括产率、水分含量、挥发物含量、灰分含量、固定碳含量、苯吸附量、亚甲基蓝吸附量和碘吸附量。结果表明,生产的活性炭具有以下质量参数:产率 75.61%;水分含量 1.27%;挥发物含量 9.98%;灰分含量 5.43%;固定碳含量 84.58%;苯吸附能力 8.58%;亚甲基蓝吸附能力 87.73 mg/g;碘吸附能力 948.19 mg/g。由此可以得出结论,从 FTS 废弃物中提取的活性炭具有良好的碘吸附性,符合 SNI 06-3730-1995 质量标准。由于 FTS 废弃物活性炭的碘吸附能力,将 FTS 废弃物转化为活性炭是提高该材料价值的一种潜在方法。
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引用次数: 0
Determination of the Boundary between Juvenile–Mature Wood of Diospyros kaki and Their Wood Anatomical Variations 确定 Diospyros kaki 幼木与成熟木的界限及其木质解剖变化
Q2 Engineering Pub Date : 2024-03-01 DOI: 10.5658/wood.2024.52.2.191
Eka Kartikawati, Bienitta, Fanany Wuri Prastiwi, W. D. Nugroho
Persimmon wood ( Diospyros kaki ) is a seasonal fruit-producing plant with a beautiful dark pattern in its wood that is suitable for high-quality furniture, sculptures and musical instruments. The utilization of persimmon wood can be improved by determining its anatomical characteristics, such as juvenile and mature wood. This study aimed to determine the boundaries between juvenile and mature wood and observe the anatomical properties of juvenile and mature wood and their variations in the axial direction. Three 30-year-old persimmon ( D. kaki ) trees grown in Karo, North Sumatra, Indonesia, were used in this study. The boundary between juvenile and mature wood was determined by measuring the fiber length and vessel element length from near the pith to near the bark. Anatomical observations were conducted in the juvenile and mature wood areas. The results showed that the average boundaries between juvenile and mature wood were 44.11 mm from the pith and were not significantly different in the axial direction of the trees. Furthermore, the wood anatomy categories of juvenile and mature wood differed significantly in terms of fiber diameter, fiber proportion, vessel proportion, and axial parenchyma proportion. In the axial direction, vessel diameter, ray parenchyma frequency, and ray parenchyma proportion at the base, middle, and top of the tree were significantly different.
柿木(Diospyros kaki)是一种季节性水果生产植物,其木材具有美丽的深色花纹,适合制作高品质的家具、雕塑和乐器。通过确定柿木的解剖特征(如幼木和成熟木),可以提高柿木的利用率。本研究旨在确定柿木幼年期和成熟期的界限,并观察柿木幼年期和成熟期的解剖特性及其轴向变化。本研究使用了生长在印度尼西亚北苏门答腊卡洛的三棵 30 年树龄的柿树(D. kaki)。通过测量从髓部附近到树皮附近的纤维长度和血管元素长度,确定了幼木和成熟木之间的界限。对幼木和成熟木区域进行了解剖观察。结果表明,幼木和成熟木的平均边界距离髓部 44.11 毫米,并且在树木的轴向方向上没有显著差异。此外,幼木和成熟木的木材解剖类别在纤维直径、纤维比例、血管比例和轴向实质结构比例方面存在显著差异。在轴向方向上,树木基部、中部和顶部的血管直径、射线实质频率和射线实质比例均有显著差异。
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引用次数: 0
Physical and Mechanical Properties of Light Red Meranti Treated with Boron Preservatives 用硼防腐剂处理过的浅红色柳桉的物理和机械特性
Q2 Engineering Pub Date : 2024-03-01 DOI: 10.5658/wood.2024.52.2.157
Man Djun Lee, Ridge Wei Cheong Tang, Zeno Michael, Miqdad Khairulmaini, Azmi Roslan, Ahmad Faidzal Khodori, Hazim Sharudin, Pui San Lee
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引用次数: 0
Fixed Bed Drying of Sugarcane Bagasse Using Solar Energy 利用太阳能固定床干燥甘蔗渣
Q2 Engineering Pub Date : 2024-01-01 DOI: 10.5658/wood.2024.52.1.47
Hyoung-Woo Lee, Hyun-Ook Kim, Dong-Hoon Lee, Don-Ha Choi, Seung-Gyu Kim
Solar energy is one of the most promising options for renewable energy and biomass is one of them. One of the main biomass sources, sugarcane bagasse, is produced annually in more than hundreds of nations worldwide exceeding 4.25 billion tons. To dry a 900-mm deep fixed bed of wet sugarcane bagasse, a solar air heater with a collector area of 2 m 2 was installed. Between October 10 th to 19 th in Gwangju, South Korea, a 9-day drying period, the solar collector received a total of 496,145 kJ of solar radiation. During this time, 54.5 kg of water was extracted from 133 kg of wet sugarcane bagasse (average green moisture content of 47.6% w
太阳能是最有前途的可再生能源之一,生物质能也是其中之一。甘蔗渣是主要的生物质能源之一,全世界数百个国家每年生产的甘蔗渣超过 42.5 亿吨。为了干燥 900 毫米深的湿甘蔗渣固定床,安装了一个集热器面积为 2 米 2 的太阳能空气加热器。10 月 10 日至 19 日,在韩国光州进行的为期 9 天的干燥过程中,太阳能集热器共接收了 496 145 千焦的太阳辐射。在此期间,从 133 公斤湿甘蔗渣(平均绿色含水量为 47.6% w)中提取了 54.5 公斤水。
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引用次数: 0
Evaluation of Adhesive Characteristics of Mixed Cross Laminated Timber (CLT) Using Yellow Popular and Softwood Structural Lumbers 使用黄色流行木材和软木结构木材评估混合交叉层压木材 (CLT) 的粘合特性
Q2 Engineering Pub Date : 2024-01-01 DOI: 10.5658/wood.2024.52.1.58
Keon-Ho Kim, Hyun-Mi Lee, Min Lee
.
.
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引用次数: 0
Drying Efficiency of Betung Bamboo Strips (Dendrocalamus asper) Based on Different Solar Drying Oven Designs 基于不同太阳能干燥炉设计的贝东竹条(Dendrocalamus asper)干燥效率
Q2 Engineering Pub Date : 2024-01-01 DOI: 10.5658/wood.2024.52.1.1
I. Sumardi, Anggit Kusuma Dewan Daru, Alfi Rumidatul, R. Dungani, Y. Suhaya, Neil Prihanto, Rudi Hartono
Betung bamboo ( Dendrocalamus asper ) is used as a building and handicraft material in Indonesia; however, bamboo needs to be dried to increase its stability. This study aimed to evaluate the efficiency of drying bamboo using solar energy and different drying oven designs. The betung bamboo pieces were dried using a direct solar dryer (direct drying) and an indirect solar dryer (indirect drying) and then the decrease in levels that occurred based on the relative humidity (RH) and temperature values achieved in the two dryers were compared. The highest average temperature in the direct indirect drying oven compartment was 60.1 ± 13.1℃ with 19.9 ± 16.4% RH and 60.2 ± 11.9℃ with 19.5 ± 15.5% RH, respectively. The drying defect in indirect drying was lower than that in direct drying, and indirect drying had a 61.7% greater average water loss than direct drying with significant difference (95%, analysis of variance) based on water loss/compartment volume parameters. Thus, the solar drying oven can be used to air-dry bamboo (14%) for 7 d from an initial moisture content of 70%–80% in bamboo strips. The results of this research can be used for small-scale bamboo processing industries that have limited use of electrical energy with quite good results.
竹子(Dendrocalamus asper)在印度尼西亚被用作建筑和手工艺品材料,但竹子需要经过干燥才能提高其稳定性。本研究旨在评估利用太阳能和不同烘干炉设计烘干竹子的效率。使用太阳能直接烘干机(直接烘干)和太阳能间接烘干机(间接烘干)对槟榔竹片进行烘干,然后比较了两种烘干机达到的相对湿度(RH)和温度值的降低程度。在直接间接干燥箱中,最高平均温度分别为 60.1 ± 13.1℃(相对湿度为 19.9 ± 16.4%)和 60.2 ± 11.9℃(相对湿度为 19.5 ± 15.5%)。间接干燥的干燥缺陷低于直接干燥,间接干燥的平均失水率比直接干燥高 61.7%,根据失水率/隔室容积参数,差异显著(95%,方差分析)。因此,太阳能干燥箱可用于对初始含水量为 70%-80% 的竹条进行 7 天的风干竹材(14%)。该研究成果可用于电能使用有限的小型竹材加工业,并取得了相当好的效果。
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引用次数: 0
期刊
Journal of the Korean wood science and technology
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