Pub Date : 2023-06-30DOI: 10.4038/jnsfsr.v51i2.11232
W. Samarasinghe, K. H. Jayawardena, C. Ranasinghe, S. Somaratne, G. Gunaherath
Non-healing wounds are a global health problem. Substances that enhance cell proliferation, angiogenesis, and prevention of bacterial infections accelerate the wound healing process. In this study, the wound healing potential of Ziziphus oenoplia, is investigated for its ability in cell proliferation, angiogenesis, and antibacterial potential. The potential of cell proliferation enhancement (mean percent wound closure) and angiogenic response (mean vascular index) of hexanes, dichloromethane, ethyl acetate, and methanol extracts of leaf and bark of Z. oenoplia were evaluated by scratch wound assay (SWA) using Madin-Darby Canine Kidney (MDCK) cells and chick chorioallantoic membrane (CAM) assay, respectively. The antibacterial activity of these extracts was evaluated against Bacillus subtilis, Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa by disc diffusion method. Enhanced cell proliferation was shown by the hexanes, dichloromethane, and ethyl acetate extracts of leaf and the hexanes extract of bark. An enhanced angiogenic response was shown by the methanol and ethyl acetate extracts of leaves and the methanol and hexanes extracts of bark. Dichloromethane extract of both leaf and bark showed considerable antibacterial activity against P. aeruginosa which is less susceptible to common antibiotics. SWA-directed fractionation of the hexanes extract of the leaf has resulted in the isolation and identification of an active fraction showing mean percent wound closure of 86.4% (positive control 90.2%) and mean vascular index of 34.5 (positive control 48.6). This fraction contained lupeol, α-amyrin, β-amyrin, hexacosanol, and octacosanol. The present study provides supportive evidence for the potential of wound healing enhancement of leaf and bark extracts of Z. oenoplia.
{"title":"In-vitro wound healing potential of Ziziphus oenoplia (L.) Miller","authors":"W. Samarasinghe, K. H. Jayawardena, C. Ranasinghe, S. Somaratne, G. Gunaherath","doi":"10.4038/jnsfsr.v51i2.11232","DOIUrl":"https://doi.org/10.4038/jnsfsr.v51i2.11232","url":null,"abstract":"\u0000Non-healing wounds are a global health problem. Substances that enhance cell proliferation, angiogenesis, and prevention of bacterial infections accelerate the wound healing process. In this study, the wound healing potential of Ziziphus oenoplia, is investigated for its ability in cell proliferation, angiogenesis, and antibacterial potential. The potential of cell proliferation enhancement (mean percent wound closure) and angiogenic response (mean vascular index) of hexanes, dichloromethane, ethyl acetate, and methanol extracts of leaf and bark of Z. oenoplia were evaluated by scratch wound assay (SWA) using Madin-Darby Canine Kidney (MDCK) cells and chick chorioallantoic membrane (CAM) assay, respectively. The antibacterial activity of these extracts was evaluated against Bacillus subtilis, Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa by disc diffusion method. Enhanced cell proliferation was shown by the hexanes, dichloromethane, and ethyl acetate extracts of leaf and the hexanes extract of bark. An enhanced angiogenic response was shown by the methanol and ethyl acetate extracts of leaves and the methanol and hexanes extracts of bark. Dichloromethane extract of both leaf and bark showed considerable antibacterial activity against P. aeruginosa which is less susceptible to common antibiotics. SWA-directed fractionation of the hexanes extract of the leaf has resulted in the isolation and identification of an active fraction showing mean percent wound closure of 86.4% (positive control 90.2%) and mean vascular index of 34.5 (positive control 48.6). This fraction contained lupeol, α-amyrin, β-amyrin, hexacosanol, and octacosanol. The present study provides supportive evidence for the potential of wound healing enhancement of leaf and bark extracts of Z. oenoplia.","PeriodicalId":17429,"journal":{"name":"Journal of the National Science Foundation of Sri Lanka","volume":" ","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48328070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-30DOI: 10.4038/jnsfsr.v51i2.11177
S. Y. Liew, Y. Sivasothy, N. N. Shaikh, K. Javaid, DM Isa, V. Lee, M. Choudhary, K. Awang
The most prevalent subtype of diabetes is Type 2 Diabetes Mellitus which results from an abnormal postprandial increase in blood glucose. Inhibition of the carbohydrate-hydrolysing enzymes (alpha-glucosidase and alpha-amylase) in the human digestive organs can control blood glucose levels, making it an important strategy in the management of Type 2 Diabetes Mellitus. A majority of the oral synthetic drugs which have been developed to treat Type 2 Diabetes Mellitus are expensive and have undesirable side effects. As a result, plant-derived remedies have become preferred alternatives as they are easily available, affordable and less harmful. Angustine (1) the major constituent in Nauclea subdita (Korth.) Steud., and (E)-labda-8(17),12-dien-15,16-dial (2) and zerumin A (3) of Alpinia pahangensis Ridley were evaluated for their α-glucosidase inhibitory activity. (E)-Labda-8(17),12-dien-15,16-dial (2) (IC50 = 39.7 μM) was identified as the most potent inhibitor among all three, followed by angustine (1) (IC50 = 48.1 μM) and zerumin A (3) (IC50 = 53.3 μM). Enzyme kinetic studies indicated that angustine (1) was a mixed-type inhibitor, while (E)-labda-8(17),12-dien-15,16-dial (2), and zerumin A (3) were non-competitive inhibitors. Molecular docking and molecular dynamics (MD) simulation studies were performed to predict the key interactions between the ligands and the target protein, 1AGM (complex of acarbose with glucoamylase from Aspergillus awamori). The results of the study of binding interaction energy suggested that angustine (1) has the potential to be used as a natural drug lead in the treatment of type 2 diabetes mellitus.
{"title":"Natural α-glucosidase inhibitors from selected medicinal plants in Malaysia","authors":"S. Y. Liew, Y. Sivasothy, N. N. Shaikh, K. Javaid, DM Isa, V. Lee, M. Choudhary, K. Awang","doi":"10.4038/jnsfsr.v51i2.11177","DOIUrl":"https://doi.org/10.4038/jnsfsr.v51i2.11177","url":null,"abstract":"\u0000The most prevalent subtype of diabetes is Type 2 Diabetes Mellitus which results from an abnormal postprandial increase in blood glucose. Inhibition of the carbohydrate-hydrolysing enzymes (alpha-glucosidase and alpha-amylase) in the human digestive organs can control blood glucose levels, making it an important strategy in the management of Type 2 Diabetes Mellitus. A majority of the oral synthetic drugs which have been developed to treat Type 2 Diabetes Mellitus are expensive and have undesirable side effects. As a result, plant-derived remedies have become preferred alternatives as they are easily available, affordable and less harmful. Angustine (1) the major constituent in Nauclea subdita (Korth.) Steud., and (E)-labda-8(17),12-dien-15,16-dial (2) and zerumin A (3) of Alpinia pahangensis Ridley were evaluated for their α-glucosidase inhibitory activity. (E)-Labda-8(17),12-dien-15,16-dial (2) (IC50 = 39.7 μM) was identified as the most potent inhibitor among all three, followed by angustine (1) (IC50 = 48.1 μM) and zerumin A (3) (IC50 = 53.3 μM). Enzyme kinetic studies indicated that angustine (1) was a mixed-type inhibitor, while (E)-labda-8(17),12-dien-15,16-dial (2), and zerumin A (3) were non-competitive inhibitors. Molecular docking and molecular dynamics (MD) simulation studies were performed to predict the key interactions between the ligands and the target protein, 1AGM (complex of acarbose with glucoamylase from Aspergillus awamori). The results of the study of binding interaction energy suggested that angustine (1) has the potential to be used as a natural drug lead in the treatment of type 2 diabetes mellitus.","PeriodicalId":17429,"journal":{"name":"Journal of the National Science Foundation of Sri Lanka","volume":" ","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45805696","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-30DOI: 10.4038/jnsfsr.v51i2.11230
W. Wanninayake, D. Namawardana, R. Wanigasekara, K. Jayathilaka, R. P. Wijesundera, W. Siripala, M. I. Malik
Polymer solar cells (PSCs) have attracted tremendous interest as suitable candidates for harnessing solar energy in the recent years. The inherent optoelectronic properties of the inorganic transition metal oxide, negative type cuprous oxide (n-Cu2O), makes it an attractive candidate to improve the performance of PSCs when incorporated as the electron selective transport layers (ESTLs) in the device. In this study, inverted PSCs were fabricated on stainless steel (SS) substrates with n-Cu2O as the ESTL. The n-Cu2O films were prepared by electrodeposition method, followed by annealing under ambient conditions. The active layer material was prepared as bulk heterojunction blend using regioregular poly(3-hexylthiophene) (P3HT) and phenyl-C61-butyric acid methyl ester (PCBM). Poly-(4,3-ethylene dioxythiophene):poly(styrenesulphonate) (PEDOT:PSS) was used as the hole transport layer (HTL) and the final device structure was SS/n-Cu2O/P3HT:PCBM/PEDOT:PSS/Au. Annealing of the n-Cu2O ESTL in air was optimized observing the photoactive performance of the device. Optoelectronic performance of the devices was characterized using spectral response and dark and light current-voltage (I-V) measurements. n-Cu2O ESTL- incorporated devices have absorbed more photons in the short wavelength region of 450‒600 nm with the annealing of n-Cu2O ESTL due to the reduction of electron-hole recombination. The performance of the devices was significantly increased after incorporating pre-annealed n-Cu2O ESTL at 175 °C for 30 min in air. The maximum power conversion efficiency (PCE) was 0.35%.
{"title":"Efficiency boost of inverted polymer solar cells using electrodeposited n-type Cu2O electrons selective transport layers (ESTLs)","authors":"W. Wanninayake, D. Namawardana, R. Wanigasekara, K. Jayathilaka, R. P. Wijesundera, W. Siripala, M. I. Malik","doi":"10.4038/jnsfsr.v51i2.11230","DOIUrl":"https://doi.org/10.4038/jnsfsr.v51i2.11230","url":null,"abstract":"\u0000Polymer solar cells (PSCs) have attracted tremendous interest as suitable candidates for harnessing solar energy in the recent years. The inherent optoelectronic properties of the inorganic transition metal oxide, negative type cuprous oxide (n-Cu2O), makes it an attractive candidate to improve the performance of PSCs when incorporated as the electron selective transport layers (ESTLs) in the device. In this study, inverted PSCs were fabricated on stainless steel (SS) substrates with n-Cu2O as the ESTL. The n-Cu2O films were prepared by electrodeposition method, followed by annealing under ambient conditions. The active layer material was prepared as bulk heterojunction blend using regioregular poly(3-hexylthiophene) (P3HT) and phenyl-C61-butyric acid methyl ester (PCBM). Poly-(4,3-ethylene dioxythiophene):poly(styrenesulphonate) (PEDOT:PSS) was used as the hole transport layer (HTL) and the final device structure was SS/n-Cu2O/P3HT:PCBM/PEDOT:PSS/Au. Annealing of the n-Cu2O ESTL in air was optimized observing the photoactive performance of the device. Optoelectronic performance of the devices was characterized using spectral response and dark and light current-voltage (I-V) measurements. n-Cu2O ESTL- incorporated devices have absorbed more photons in the short wavelength region of 450‒600 nm with the annealing of n-Cu2O ESTL due to the reduction of electron-hole recombination. The performance of the devices was significantly increased after incorporating pre-annealed n-Cu2O ESTL at 175 °C for 30 min in air. The maximum power conversion efficiency (PCE) was 0.35%.","PeriodicalId":17429,"journal":{"name":"Journal of the National Science Foundation of Sri Lanka","volume":" ","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41776535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-11DOI: 10.4038/jnsfsr.v51i1.11121
T. Kariyawasam, D. Nanayakkara, A. Sumanarathna, D. Weerasinghe, L. Suriyagoda, D. Jayatilake
{"title":"Assessment of submergence stress responses and mining allelic variations of submergence tolerance gene Sub1A in Sri Lankan rice germplasm","authors":"T. Kariyawasam, D. Nanayakkara, A. Sumanarathna, D. Weerasinghe, L. Suriyagoda, D. Jayatilake","doi":"10.4038/jnsfsr.v51i1.11121","DOIUrl":"https://doi.org/10.4038/jnsfsr.v51i1.11121","url":null,"abstract":"","PeriodicalId":17429,"journal":{"name":"Journal of the National Science Foundation of Sri Lanka","volume":" ","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45510264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-10DOI: 10.4038/jnsfsr.v51i1.10870
L. Chamath, L. Srimal, G. Sewvandi
{"title":"Assessment of transverse thermal conductivity of coir fibre using experimental, analytical, and numerical methods","authors":"L. Chamath, L. Srimal, G. Sewvandi","doi":"10.4038/jnsfsr.v51i1.10870","DOIUrl":"https://doi.org/10.4038/jnsfsr.v51i1.10870","url":null,"abstract":"","PeriodicalId":17429,"journal":{"name":"Journal of the National Science Foundation of Sri Lanka","volume":" ","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43939100","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-10DOI: 10.4038/jnsfsr.v51i1.10844
V. A. Rani, G. Guna, D. Prabhakaran, M. Thirumarimurugan
{"title":"Temperature control in an exothermic continuous stirred tank reactor","authors":"V. A. Rani, G. Guna, D. Prabhakaran, M. Thirumarimurugan","doi":"10.4038/jnsfsr.v51i1.10844","DOIUrl":"https://doi.org/10.4038/jnsfsr.v51i1.10844","url":null,"abstract":"","PeriodicalId":17429,"journal":{"name":"Journal of the National Science Foundation of Sri Lanka","volume":" ","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46218304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-10DOI: 10.4038/jnsfsr.v51i1.10469
{"title":"On recurrence relations for moments of dual generalized order statistics for a general transmuted power function distributions with characterizations","authors":"","doi":"10.4038/jnsfsr.v51i1.10469","DOIUrl":"https://doi.org/10.4038/jnsfsr.v51i1.10469","url":null,"abstract":"","PeriodicalId":17429,"journal":{"name":"Journal of the National Science Foundation of Sri Lanka","volume":" ","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46766483","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-10DOI: 10.4038/jnsfsr.v51i1.11667
{"title":"Predatory Journals; be aware of the spectrum!","authors":"","doi":"10.4038/jnsfsr.v51i1.11667","DOIUrl":"https://doi.org/10.4038/jnsfsr.v51i1.11667","url":null,"abstract":"","PeriodicalId":17429,"journal":{"name":"Journal of the National Science Foundation of Sri Lanka","volume":" ","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47513277","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-10DOI: 10.4038/jnsfsr.v51i1.10752
{"title":"Mechanically exfoliated graphene from Sri Lankan vein graphite for field effect transistor application","authors":"","doi":"10.4038/jnsfsr.v51i1.10752","DOIUrl":"https://doi.org/10.4038/jnsfsr.v51i1.10752","url":null,"abstract":"","PeriodicalId":17429,"journal":{"name":"Journal of the National Science Foundation of Sri Lanka","volume":" ","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48238349","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-10DOI: 10.4038/jnsfsr.v51i1.10730
{"title":"A comparison of model parameter estimation methods for complex survey survival data","authors":"","doi":"10.4038/jnsfsr.v51i1.10730","DOIUrl":"https://doi.org/10.4038/jnsfsr.v51i1.10730","url":null,"abstract":"","PeriodicalId":17429,"journal":{"name":"Journal of the National Science Foundation of Sri Lanka","volume":" ","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42866237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：