Pub Date : 2019-03-01DOI: 10.19106/JMEDSCIE/005101201901
H. Hariawan, M. Soesatyo, M. Munawar, A. Ghozali
In-stent restenosis after stenting in vascular occurs secondary to the accumulation of smooth muscle cell and extracellular matrix. This condition is a major complication caused by the occurrence of neointimal hyperplasia (NIH). The study aimed to prove the role of miRNA-21 as a risk predictor of the NIH event in aorta of rabbits animal model who underwent bare metal (BM) type stent implantation and to know the miRNA-21 role in IL-6 and IL-8 expressions. This study was quasi experimental, coducted in Catheterization Laboratory Dr. Sardjito General Hospital Yogyakarta. Stent implantation was observed intravascular ultrasonography. Blood vessel which was already deployed by stent taken as sample for pathologic examination. Rabbit blood samples were collected on day 0, 7, and 28. Quantification miRNA-21 expression has been done with qPCR and ELISA approach. These 10 rabbits divided into two groups in which one group as control (without stent deployment, 4 models) and another group as intervention (with BM type stent deployment, 6 models). The increase in expression of miRNA-21 on the day 7 and day 28 in the intervention group compared to the control group. Neointimal hyperplasia increased in intervention group on day 7, and 28 were from mild grade to moderate, and severe respectively. In addition, levels of IL-6 and IL-8 increased on day 28 compared with on day 7. This study showed increase of miRNA-21 expression on day 7 and NIH rise from the mild to moderate. Furthermore, on day 28, that increases of miRNA-21 expression and NIH rose from to moderate to severe. The increase of miRNA-21 was also shown on day 7 and 28 followed by the increase of IL-6 and IL-8 levels.
{"title":"Correlation between micro-RNA-21 expression and inflammation cytokine in rabbits implanted with bare metal stent with the incidence of neo intimal hyperplasia","authors":"H. Hariawan, M. Soesatyo, M. Munawar, A. Ghozali","doi":"10.19106/JMEDSCIE/005101201901","DOIUrl":"https://doi.org/10.19106/JMEDSCIE/005101201901","url":null,"abstract":"In-stent restenosis after stenting in vascular occurs secondary to the accumulation of smooth muscle cell and extracellular matrix. This condition is a major complication caused by the occurrence of neointimal hyperplasia (NIH). The study aimed to prove the role of miRNA-21 as a risk predictor of the NIH event in aorta of rabbits animal model who underwent bare metal (BM) type stent implantation and to know the miRNA-21 role in IL-6 and IL-8 expressions. This study was quasi experimental, coducted in Catheterization Laboratory Dr. Sardjito General Hospital Yogyakarta. Stent implantation was observed intravascular ultrasonography. Blood vessel which was already deployed by stent taken as sample for pathologic examination. Rabbit blood samples were collected on day 0, 7, and 28. Quantification miRNA-21 expression has been done with qPCR and ELISA approach. These 10 rabbits divided into two groups in which one group as control (without stent deployment, 4 models) and another group as intervention (with BM type stent deployment, 6 models). The increase in expression of miRNA-21 on the day 7 and day 28 in the intervention group compared to the control group. Neointimal hyperplasia increased in intervention group on day 7, and 28 were from mild grade to moderate, and severe respectively. In addition, levels of IL-6 and IL-8 increased on day 28 compared with on day 7. This study showed increase of miRNA-21 expression on day 7 and NIH rise from the mild to moderate. Furthermore, on day 28, that increases of miRNA-21 expression and NIH rose from to moderate to severe. The increase of miRNA-21 was also shown on day 7 and 28 followed by the increase of IL-6 and IL-8 levels.","PeriodicalId":17474,"journal":{"name":"Journal of thee Medical Sciences (Berkala Ilmu Kedokteran)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89624384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-01DOI: 10.19106/jmedsci005102201908
Wahyu Priyatno, Supomo
Heart valve disease is a serious health probem due its significant effect on mortality.Surgical intervention plays an importan roleinthe management of moderate to the severe valvular heart disease. The evaluation of the patientoutcomes has been widely accepted as one of the important steps to improve the quality of patient care.Recognition of post operative complications might significantly affect the patient quality of life.Therefore, the identification of prognostic factors and morbidity of heart surgery patients can provide valuable insights on improving the quality of patient care.This study aimedto investigat the prognostic factors for heart valve surgery outcomes in Dr. Sardjito General Hospital, Yogyakarta, Indonesia. This study was conducted using data ofpatient’s medical records who underwent heart valve surgeryincluded morbidities and their prognostic factors.The data were analyzed using Chi-square test and logistic regression with significance level of 0.05. During 2010 to 2014, 82 patients underwent heart valve surgeries at the hospital. The kidney disorders and heart failure were significantly associated with the patient morbidity (p 0.05). Multivariate analysis showed that the heart failure is the most significant factor of morbidity. The risk of New York Heart Association (NYHA) class 3 patients were 7.18 times compared with NYHA class 2 patients. In conclusion, the heart failure is the most significant factor for the morbidityof heart valve surgery followed by the kidney disorders at the Dr. Sardjito General Hospital.
{"title":"Prognostic factors for heart valve surgery outcomes in Dr Sardjito General Hospital Yogyakarta, Indonesia","authors":"Wahyu Priyatno, Supomo","doi":"10.19106/jmedsci005102201908","DOIUrl":"https://doi.org/10.19106/jmedsci005102201908","url":null,"abstract":"Heart valve disease is a serious health probem due its significant effect on mortality.Surgical intervention plays an importan roleinthe management of moderate to the severe valvular heart disease. The evaluation of the patientoutcomes has been widely accepted as one of the important steps to improve the quality of patient care.Recognition of post operative complications might significantly affect the patient quality of life.Therefore, the identification of prognostic factors and morbidity of heart surgery patients can provide valuable insights on improving the quality of patient care.This study aimedto investigat the prognostic factors for heart valve surgery outcomes in Dr. Sardjito General Hospital, Yogyakarta, Indonesia. This study was conducted using data ofpatient’s medical records who underwent heart valve surgeryincluded morbidities and their prognostic factors.The data were analyzed using Chi-square test and logistic regression with significance level of 0.05. During 2010 to 2014, 82 patients underwent heart valve surgeries at the hospital. The kidney disorders and heart failure were significantly associated with the patient morbidity (p 0.05). Multivariate analysis showed that the heart failure is the most significant factor of morbidity. The risk of New York Heart Association (NYHA) class 3 patients were 7.18 times compared with NYHA class 2 patients. In conclusion, the heart failure is the most significant factor for the morbidityof heart valve surgery followed by the kidney disorders at the Dr. Sardjito General Hospital.","PeriodicalId":17474,"journal":{"name":"Journal of thee Medical Sciences (Berkala Ilmu Kedokteran)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86933064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-01DOI: 10.19106/jmedsci005102201906
Rosmeri Handayani, Siti Muchayat Purnamaningsih, U. Sukorini
Non-alcoholic fatty liver disease (NAFLD) is liver disorders characterized by macrovesicular fatty liver, fibrosis, cirrhosis that not associated with alcohol consumption. The prevalence of NAFLD has risen with a pandemic of obesity. The increase of free fatty acid (FFA) oxidation will induce endoplasmic reticulum stress that cause mitochondrial dysfunction and lead to increase reactive oxygen species (ROS) production causing apoptosis of liver cells. The aim of study was to determine the prevalence of FFA in the obese group. This was an observational analytical study with cross-sectional design to determine the prevalence ratio of FFA in the obese group with NAFLD compared to the group without NAFLD. Obese women who fulfill the inclusion and exclusion criteria were involved in this study. Five mL venous blood sample was collected for the measurement of lipid profile, liver enzyme and FFA. Fatty liver was evaluated using abdominal USG. The Chi-square test was used to analyze different proportions of FFA between the both groups. Sixty four subjects were participated in this study and classified into obese with NAFLD (39 subjects) and obese without NAFLD (25 subjects). The prevalence ratio of FFA with cutoff value ≥2.66 nmol/mL in the obese group with NAFLD was 4.3 times higher than those without NAFLD (95% IC: 3.5 – 42.3; p<0.001).
{"title":"Prevalence ratio of free fatty acid in obese group with non-alcoholic fatty liver disease","authors":"Rosmeri Handayani, Siti Muchayat Purnamaningsih, U. Sukorini","doi":"10.19106/jmedsci005102201906","DOIUrl":"https://doi.org/10.19106/jmedsci005102201906","url":null,"abstract":"Non-alcoholic fatty liver disease (NAFLD) is liver disorders characterized by macrovesicular fatty liver, fibrosis, cirrhosis that not associated with alcohol consumption. The prevalence of NAFLD has risen with a pandemic of obesity. The increase of free fatty acid (FFA) oxidation will induce endoplasmic reticulum stress that cause mitochondrial dysfunction and lead to increase reactive oxygen species (ROS) production causing apoptosis of liver cells. The aim of study was to determine the prevalence of FFA in the obese group. This was an observational analytical study with cross-sectional design to determine the prevalence ratio of FFA in the obese group with NAFLD compared to the group without NAFLD. Obese women who fulfill the inclusion and exclusion criteria were involved in this study. Five mL venous blood sample was collected for the measurement of lipid profile, liver enzyme and FFA. Fatty liver was evaluated using abdominal USG. The Chi-square test was used to analyze different proportions of FFA between the both groups. Sixty four subjects were participated in this study and classified into obese with NAFLD (39 subjects) and obese without NAFLD (25 subjects). The prevalence ratio of FFA with cutoff value ≥2.66 nmol/mL in the obese group with NAFLD was 4.3 times higher than those without NAFLD (95% IC: 3.5 – 42.3; p<0.001).","PeriodicalId":17474,"journal":{"name":"Journal of thee Medical Sciences (Berkala Ilmu Kedokteran)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89436659","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-03-06DOI: 10.19106/jmedsci005103201907
Hifdza Faza Felisha, H. Rinonce, S. Anwar, E. Dwianingsih
Introduction: Breast lump is a very common complaint in woman, especially during reproductive year. Fine Needle Aspiration Biopsy (FNAB) is less invasive procedure, usually performed as initial diagnosis prior to operative procedure. The accuracy of the FNAB in Indonesia needs to be elaborated. Aim: To evaluate the sensitivity and specificity of FNAB in diagnosing breast neoplasm. Methods: This research is a retrospective study with cross sectional design, involving 145 patients with breast lump who underwent FNAB and histopathology examination in Sardjito Hospital, Yogyakarta, from 2012 to 2014. Results: Data analysis showed that female to male ratio was 23.2:1, commonly occurred at 41-50 years old. Forty one cases (28.28%) were diagnosed as benign lesion with fibrocystic changes as the commonest case (11.19%). Malignant case was 104 cases (71.72%) with ductal carcinoma as the highest case (51.49%). FNAB achieved a sensitivity of 85.58%, a specificity of 100% and a total accuracy of 89.66% in determining the benign or malignant breast lump. The accuracy, sensitivity and specificity of FNAB in diagnosing ductal carcinoma were 83.58%, 85.51% and 81.54%, respectively. The accuracy, sensitivity and specificity of FNAB to diagnose fibrocystic changes lesion were 85.82%, 26.67% and 93.28%, respectively. Conclusion: FNAB can be used as an alternative diagnostic tool to diagnose breast neoplasm. It provides rapid, cheaper (economical), effective, valuable, and less invasive procedure in breast lump diagnosis. Keywords: FNAB, breast lump, sensitivity, specificity
{"title":"The accuracy of fine needle aspiration biopsy to diagnose breast neoplasm","authors":"Hifdza Faza Felisha, H. Rinonce, S. Anwar, E. Dwianingsih","doi":"10.19106/jmedsci005103201907","DOIUrl":"https://doi.org/10.19106/jmedsci005103201907","url":null,"abstract":"Introduction: Breast lump is a very common complaint in woman, especially during reproductive year. Fine Needle Aspiration Biopsy (FNAB) is less invasive procedure, usually performed as initial diagnosis prior to operative procedure. The accuracy of the FNAB in Indonesia needs to be elaborated. Aim: To evaluate the sensitivity and specificity of FNAB in diagnosing breast neoplasm. Methods: This research is a retrospective study with cross sectional design, involving 145 patients with breast lump who underwent FNAB and histopathology examination in Sardjito Hospital, Yogyakarta, from 2012 to 2014. Results: Data analysis showed that female to male ratio was 23.2:1, commonly occurred at 41-50 years old. Forty one cases (28.28%) were diagnosed as benign lesion with fibrocystic changes as the commonest case (11.19%). Malignant case was 104 cases (71.72%) with ductal carcinoma as the highest case (51.49%). FNAB achieved a sensitivity of 85.58%, a specificity of 100% and a total accuracy of 89.66% in determining the benign or malignant breast lump. The accuracy, sensitivity and specificity of FNAB in diagnosing ductal carcinoma were 83.58%, 85.51% and 81.54%, respectively. The accuracy, sensitivity and specificity of FNAB to diagnose fibrocystic changes lesion were 85.82%, 26.67% and 93.28%, respectively. Conclusion: FNAB can be used as an alternative diagnostic tool to diagnose breast neoplasm. It provides rapid, cheaper (economical), effective, valuable, and less invasive procedure in breast lump diagnosis. Keywords: FNAB, breast lump, sensitivity, specificity","PeriodicalId":17474,"journal":{"name":"Journal of thee Medical Sciences (Berkala Ilmu Kedokteran)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85459051","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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