Pub Date : 2020-10-02DOI: 10.19106/JMEDSCI005204202006
Nelson Sudiyono, Rudy Handoyo
Carpal tunnel syndrome (CTS) is the most common neuropathy compression syndrome. The effectiveness of low-level laser therapy (LLLT) as one of the conservative therapy for CTS is still debatable. High-intensity laser therapy (HILT) is developed with higher energy and deeper tissue penetration than LLLT. This study aimed to compare the effect of HILT and LLLT on sensory and motoric electrophysiologic parameters in moderate CTS patients. This was an experimental randomized pre and post-test group study. Sixteen patients (fifteen females and one male) with moderate CTS were randomly assigned into two groups. The HILT group was given HILT with analgesic dosage 10 J/cm2 and biostimulation dosage 120 J/cm2. The LLLT group was given LLLT with dosage 6 J/cm2. All treatments were given for ten sessions in 2 weeks. Combined sensory index (CSI), sensory nerve conduction velocity (SNCV), and distal motoric latency (DML) were recorded before and three days after the last treatment. The mean decrease of CSI was more significant in HILT group (-0.37±0.37 ms; p=0.03). There were no significant differences in the mean increase of SNCV (HILT = 3.16±3.15 m/s, LLLT= 2.74±1.42 m/s; p=0.73) and mean decrease in DML between two groups (HILT = - 0.20 ± 0.18 ms , LLLT = - 0.14 ± 0.21 ms; p=0.52). In conclusion, the HILT is more effective than LLLT in improving the CSI values in moderate CTS patients.
{"title":"Comparison of high-intensity and low-level laser therapy effecton combined sensory index, sensory conduction velocity and distal motoric latency: a study in moderate carpal tunnel syndrome patients","authors":"Nelson Sudiyono, Rudy Handoyo","doi":"10.19106/JMEDSCI005204202006","DOIUrl":"https://doi.org/10.19106/JMEDSCI005204202006","url":null,"abstract":"Carpal tunnel syndrome (CTS) is the most common neuropathy compression syndrome. The effectiveness of low-level laser therapy (LLLT) as one of the conservative therapy for CTS is still debatable. High-intensity laser therapy (HILT) is developed with higher energy and deeper tissue penetration than LLLT. This study aimed to compare the effect of HILT and LLLT on sensory and motoric electrophysiologic parameters in moderate CTS patients. This was an experimental randomized pre and post-test group study. Sixteen patients (fifteen females and one male) with moderate CTS were randomly assigned into two groups. The HILT group was given HILT with analgesic dosage 10 J/cm2 and biostimulation dosage 120 J/cm2. The LLLT group was given LLLT with dosage 6 J/cm2. All treatments were given for ten sessions in 2 weeks. Combined sensory index (CSI), sensory nerve conduction velocity (SNCV), and distal motoric latency (DML) were recorded before and three days after the last treatment. The mean decrease of CSI was more significant in HILT group (-0.37±0.37 ms; p=0.03). There were no significant differences in the mean increase of SNCV (HILT = 3.16±3.15 m/s, LLLT= 2.74±1.42 m/s; p=0.73) and mean decrease in DML between two groups (HILT = - 0.20 ± 0.18 ms , LLLT = - 0.14 ± 0.21 ms; p=0.52). In conclusion, the HILT is more effective than LLLT in improving the CSI values in moderate CTS patients.","PeriodicalId":17474,"journal":{"name":"Journal of thee Medical Sciences (Berkala Ilmu Kedokteran)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91505799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-10-02DOI: 10.19106/JMEDSCI005204202002
Rima Wirenviona, R. I’tishom, Siti Khaerunnisa
Lead is a toxic material that can have negative effects on reproductive organs. Lead exposure reduces the activity of endogenous antioxidant enzymes and increases the number of oxidants in the body. High free radicals will affect spermatogenesis and result in decreased motile spermatozoa. Antioxidants are known to protect the motility of spermatozoa, and adequate antioxidants can be found in Dutch eggplants (Solanumbetaceum). This study aimed to evaluate the effect of S. betaceum on spermatozoa motility after exposure to lead acetate. This study is a true experimental design with a randomized post-test-only control group design. Forty male Balb/C mice 12 weeks old were randomly divided into 5 groups: two control groups (C0, C1) and three treatment groups (T1, T2, T3).The C0 received distilled water, and the C1 received 75 mg/kg BW lead acetate. The T1, T2 and T3 received 100, 200 and 400 mg/kg BW of S. betaceum, respectively, an hour before exposed lead acetate. The data were analyzed using one-way ANOVA with a significant level of p <0.05. A significantly increasein the mean total motility of spermatozoa in T1, T2, and T3 was reported.This study indicates that S. betaceum have a protective effect on spermatozoa motility when exposed to lead acetate.
{"title":"Ethanolic extract of Dutch eggplants (Solanum betaceum) protects spermatozoa motility exposed to lead acetate","authors":"Rima Wirenviona, R. I’tishom, Siti Khaerunnisa","doi":"10.19106/JMEDSCI005204202002","DOIUrl":"https://doi.org/10.19106/JMEDSCI005204202002","url":null,"abstract":"Lead is a toxic material that can have negative effects on reproductive organs. Lead exposure reduces the activity of endogenous antioxidant enzymes and increases the number of oxidants in the body. High free radicals will affect spermatogenesis and result in decreased motile spermatozoa. Antioxidants are known to protect the motility of spermatozoa, and adequate antioxidants can be found in Dutch eggplants (Solanumbetaceum). This study aimed to evaluate the effect of S. betaceum on spermatozoa motility after exposure to lead acetate. This study is a true experimental design with a randomized post-test-only control group design. Forty male Balb/C mice 12 weeks old were randomly divided into 5 groups: two control groups (C0, C1) and three treatment groups (T1, T2, T3).The C0 received distilled water, and the C1 received 75 mg/kg BW lead acetate. The T1, T2 and T3 received 100, 200 and 400 mg/kg BW of S. betaceum, respectively, an hour before exposed lead acetate. The data were analyzed using one-way ANOVA with a significant level of p <0.05. A significantly increasein the mean total motility of spermatozoa in T1, T2, and T3 was reported.This study indicates that S. betaceum have a protective effect on spermatozoa motility when exposed to lead acetate.","PeriodicalId":17474,"journal":{"name":"Journal of thee Medical Sciences (Berkala Ilmu Kedokteran)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76267854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-10-02DOI: 10.19106/JMEDSCI005204202008
Elanda Putri Madyaningtias, Arman Bausat, Jufri Latief, Nasser Mustari, Joko Hendarto, P. Prihantono
Fracture is defined by the loss of bone or cartilage continuity, either total or partial. Diabetes mellitus (DM) is a group of metabolic diseases characterized by hyperglycaemia resulting from defects in insulin secretion, insulin action, or both. HbA1c could be used as a diagnostic tool and determines the glycaemic control of DM patients. This study aimed to investigate the correlation between serum HbA1c levels and the healing process of postoperative fractures in DM patients. This was an observational study with a retrospective cohort design. The study population was DM patients who were admitted to the Department of Emergency, Dr. Wahidin Sudirohusodo General Hospital, Makassar due to long bone fracture within the period of September 2019-February 2020. The bone fractures healing was evaluated based on the radiographic union scale in tibial fractures (RUST) scores. The results are analysed using the Chi-square test. A total of 70DM patients who underwent fracture reduction surgery were involved in this study. Out of 35 patients with controlled DM (HbA1c 7%), and there were 13 (37.1%) patients and 22 (62.9%) patients with good and delayed union, respectively. The Chi-square test showed a significant relationship (p= 0.008). It can be concluded that the increase in HbA1c levels was related to the obstacles in bone fractures healing. Moreover, an infection contributes to the healing status of bone fractures.
{"title":"The correlation of HbA1c levels on the healing process of postoperative fractures in diabetes mellitus patients","authors":"Elanda Putri Madyaningtias, Arman Bausat, Jufri Latief, Nasser Mustari, Joko Hendarto, P. Prihantono","doi":"10.19106/JMEDSCI005204202008","DOIUrl":"https://doi.org/10.19106/JMEDSCI005204202008","url":null,"abstract":"Fracture is defined by the loss of bone or cartilage continuity, either total or partial. Diabetes mellitus (DM) is a group of metabolic diseases characterized by hyperglycaemia resulting from defects in insulin secretion, insulin action, or both. HbA1c could be used as a diagnostic tool and determines the glycaemic control of DM patients. This study aimed to investigate the correlation between serum HbA1c levels and the healing process of postoperative fractures in DM patients. This was an observational study with a retrospective cohort design. The study population was DM patients who were admitted to the Department of Emergency, Dr. Wahidin Sudirohusodo General Hospital, Makassar due to long bone fracture within the period of September 2019-February 2020. The bone fractures healing was evaluated based on the radiographic union scale in tibial fractures (RUST) scores. The results are analysed using the Chi-square test. A total of 70DM patients who underwent fracture reduction surgery were involved in this study. Out of 35 patients with controlled DM (HbA1c 7%), and there were 13 (37.1%) patients and 22 (62.9%) patients with good and delayed union, respectively. The Chi-square test showed a significant relationship (p= 0.008). It can be concluded that the increase in HbA1c levels was related to the obstacles in bone fractures healing. Moreover, an infection contributes to the healing status of bone fractures.","PeriodicalId":17474,"journal":{"name":"Journal of thee Medical Sciences (Berkala Ilmu Kedokteran)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84376730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-10-02DOI: 10.19106/JMEDSCI005204202001
Arif Yusuf Wicaksana, D. Nugrahaningsih, M. Wahyuningsih
Tagitinin C, an active constituent of Tithonia diversifolia (Hemsley) A. Gray, has been proven caninhibit the collagen deposition of keloid fibroblasts in vitro. However, its mechanism of action has not been widely studied. One possible mechanism involves growth factors and cytokines. Vascular endothelial growth factor (VEGF) and tumor necrosis factor alpha (TNF-α) play an important role in the collagen deposition. The study aimed to evaluate the effect of tagitinin C on VEGF and TNF-α expression in keloid fibroblasts culture. An experimental laboratory study using fibroblast cell lines at passages III and IV was performed. Treatments were divided into two groups i.e. the treatment groups after incubation with tagitinin Cat various concentration of 1, 0.5, 0.25, and 0.125μg/ mL for 72 h, and the control group using culture media without tagitinin C. Following after incubation, the VEGF and TNF-α levels of keloid fibroblast culture supernatant were measured by ELISA. Kruskal-Wallis test continued using Mann-Whitney test or one way Anova continued by independent t test were applied to evaluate the differences between groups. A p value of less than 0.05 was considered statistically significant. The VEGF levels significantly decreases in concentration-dependent manner after treatment of the tagitinin C at various concentrations (p<0.05). However, no significantly difference in TNF-α levels was observed (p> 0.05). In conclusion, tagitinin C decreases the VEGF expression of keloid fibroblasts. However, it has no effect on the TNF-α expression.
{"title":"Effect of tagitinin C isolated from kembang bulan [ Tithonia diversifolia (Hemsley) A. Gray] leaves on VEGF and TNF- α expressions of keloid fibroblast","authors":"Arif Yusuf Wicaksana, D. Nugrahaningsih, M. Wahyuningsih","doi":"10.19106/JMEDSCI005204202001","DOIUrl":"https://doi.org/10.19106/JMEDSCI005204202001","url":null,"abstract":"Tagitinin C, an active constituent of Tithonia diversifolia (Hemsley) A. Gray, has been proven caninhibit the collagen deposition of keloid fibroblasts in vitro. However, its mechanism of action has not been widely studied. One possible mechanism involves growth factors and cytokines. Vascular endothelial growth factor (VEGF) and tumor necrosis factor alpha (TNF-α) play an important role in the collagen deposition. The study aimed to evaluate the effect of tagitinin C on VEGF and TNF-α expression in keloid fibroblasts culture. An experimental laboratory study using fibroblast cell lines at passages III and IV was performed. Treatments were divided into two groups i.e. the treatment groups after incubation with tagitinin Cat various concentration of 1, 0.5, 0.25, and 0.125μg/ mL for 72 h, and the control group using culture media without tagitinin C. Following after incubation, the VEGF and TNF-α levels of keloid fibroblast culture supernatant were measured by ELISA. Kruskal-Wallis test continued using Mann-Whitney test or one way Anova continued by independent t test were applied to evaluate the differences between groups. A p value of less than 0.05 was considered statistically significant. The VEGF levels significantly decreases in concentration-dependent manner after treatment of the tagitinin C at various concentrations (p<0.05). However, no significantly difference in TNF-α levels was observed (p> 0.05). In conclusion, tagitinin C decreases the VEGF expression of keloid fibroblasts. However, it has no effect on the TNF-α expression.","PeriodicalId":17474,"journal":{"name":"Journal of thee Medical Sciences (Berkala Ilmu Kedokteran)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90922759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-10-02DOI: 10.19106/JMEDSCI005204202005
Daniel Mahendra Krisna, Akhada Maulana, Erwin Kresnoadi
Bladder obstruction due to benign prostatic hyperplasia (BPH) causes bladder walls alteration such as trabecular and diverticula. Although it is asymptomatic, however it affects the surgery complication. The aim of this study was to evaluate the correlation between bladder wall alteration and post-surgery complications in BPH patients. It was a descriptive observational study with cross-sectional design involving BPH patients who underwent histopathological examination and surgical intervention in the Bhayangkara Hospital Mataram from January 2010-December 2014. Data of the patients including age, place of birth, intraoperative finding, comorbid condition, and post-surgery complication were obtained from the medical record. The relationship between variables was analyzed using Pearson correlation test and linear regression test.A total 114 subjects were involved in this study with the most common age was in the range 60-69 years (43.0%). Seventy-fivesubjects (74.3%) had trans urethra resection of prostate (TURP). Trabecula (28.7%), diverticula (4.0%), and bladder stone (10.9%) were found when the surgical intervention performed. The most common post-surgical complication was chip or clot retention (56.4%). Significantly relationship betweenintraoperative finding with post-surgical complication was observed (r= 0.265; R2 = 0.07; p<0.05). In conclusion, there is correlation between bladder wall alteration and post-surgicalcomplication in BPH patients. Early bladder wall alteration screening is suggested in BPH patients before surgical intervention performed.
{"title":"Incidental bladder wall alteration as a surgical complication risk factor in Indonesian benign prostate hyperplasia patient","authors":"Daniel Mahendra Krisna, Akhada Maulana, Erwin Kresnoadi","doi":"10.19106/JMEDSCI005204202005","DOIUrl":"https://doi.org/10.19106/JMEDSCI005204202005","url":null,"abstract":"Bladder obstruction due to benign prostatic hyperplasia (BPH) causes bladder walls alteration such as trabecular and diverticula. Although it is asymptomatic, however it affects the surgery complication. The aim of this study was to evaluate the correlation between bladder wall alteration and post-surgery complications in BPH patients. It was a descriptive observational study with cross-sectional design involving BPH patients who underwent histopathological examination and surgical intervention in the Bhayangkara Hospital Mataram from January 2010-December 2014. Data of the patients including age, place of birth, intraoperative finding, comorbid condition, and post-surgery complication were obtained from the medical record. The relationship between variables was analyzed using Pearson correlation test and linear regression test.A total 114 subjects were involved in this study with the most common age was in the range 60-69 years (43.0%). Seventy-fivesubjects (74.3%) had trans urethra resection of prostate (TURP). Trabecula (28.7%), diverticula (4.0%), and bladder stone (10.9%) were found when the surgical intervention performed. The most common post-surgical complication was chip or clot retention (56.4%). Significantly relationship betweenintraoperative finding with post-surgical complication was observed (r= 0.265; R2 = 0.07; p<0.05). In conclusion, there is correlation between bladder wall alteration and post-surgicalcomplication in BPH patients. Early bladder wall alteration screening is suggested in BPH patients before surgical intervention performed.","PeriodicalId":17474,"journal":{"name":"Journal of thee Medical Sciences (Berkala Ilmu Kedokteran)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87899145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-10-02DOI: 10.19106/JMEDSCI005204202010
T. Azeez, M. Osundina
Insulin resistance refers to the reduced physiological effects of insulin on various tissues. Insulin resistance has been implicated in the pathophysiology of non-alcoholic fatty liver disease (NAFLD), which is a spectrum of diseases ranging from hepatic steatosis on one end to steatohepatitis, liver cirrhosis and hepatocellular carcinoma on the other end. In most parts of the developed world, it is now the most commoncause of chronic liver disease and the most commonindication for liver transplantation. A similar findingis emerging in the developing world due to the rising prevalence of obesity and widespread adoption of Western lifestyles. Despite these epidemiological data, there are no universally approved medications for the treatment of NAFLD. The pathophysiological mechanisms of NAFLD essentially include adipose tissue insulin resistance, hepatic insulin resistance, inflammation and fibrosis. At the subcellular level, mitochondrial dysfunction, oxidative changes and endoplasmic reticulum dysfunction have been documented. Several drugs have been tested in vitro and in animal studies to target these pathophysiological mechanisms. Some are presently going through clinical trials, while others have already gone through clinical trials with variable results. Other potential target sites of drug development for the treatment of NAFLD are based on the complex pathophysiology of the disease. Insulin resistance plays an important role in the development of NAFLD. There are potential targets in the pathophysiology of NAFLD that can be explored in the development of medications for the disease.
{"title":"Insulin resistance and non-alcoholic fatty liver disease: a review of the pathophysiology and the potential targets for drug actions","authors":"T. Azeez, M. Osundina","doi":"10.19106/JMEDSCI005204202010","DOIUrl":"https://doi.org/10.19106/JMEDSCI005204202010","url":null,"abstract":"Insulin resistance refers to the reduced physiological effects of insulin on various tissues. Insulin resistance has been implicated in the pathophysiology of non-alcoholic fatty liver disease (NAFLD), which is a spectrum of diseases ranging from hepatic steatosis on one end to steatohepatitis, liver cirrhosis and hepatocellular carcinoma on the other end. In most parts of the developed world, it is now the most commoncause of chronic liver disease and the most commonindication for liver transplantation. A similar findingis emerging in the developing world due to the rising prevalence of obesity and widespread adoption of Western lifestyles. Despite these epidemiological data, there are no universally approved medications for the treatment of NAFLD. The pathophysiological mechanisms of NAFLD essentially include adipose tissue insulin resistance, hepatic insulin resistance, inflammation and fibrosis. At the subcellular level, mitochondrial dysfunction, oxidative changes and endoplasmic reticulum dysfunction have been documented. Several drugs have been tested in vitro and in animal studies to target these pathophysiological mechanisms. Some are presently going through clinical trials, while others have already gone through clinical trials with variable results. Other potential target sites of drug development for the treatment of NAFLD are based on the complex pathophysiology of the disease. Insulin resistance plays an important role in the development of NAFLD. There are potential targets in the pathophysiology of NAFLD that can be explored in the development of medications for the disease.","PeriodicalId":17474,"journal":{"name":"Journal of thee Medical Sciences (Berkala Ilmu Kedokteran)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88900151","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-06-12DOI: 10.19106/jmedscisi005203202016
S. Siswanto, O. S. Utama, Agit S. Adisetiadi, Maria E. Pranasakti, M. Hakim
Coronavirus disease 2019 (COVID-19) is a worldwide outbreak caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The World Health Organization (WHO) has declared it as a public health emergency of international concern (PHEIC) and eventually a pandemic. Many clinical trials have been conducted to investigate potential and effective therapies for COVID-19. Here we reported the outcome of three COVID-19 cases treated early with the combination of hydroxychloroquine and azithromycin. Early treatments of suspected or confirmed positive COVID-19 cases with this combination therapy is to avoid disease progressions into a more severe and irreversible state. In these cases, clinical, radiological, and laboratory features were followed up. No complications were observed. The COVID-19 patients treated with this early combination therapy showed good clinical and virological responses.
{"title":"Early hydroxychloroquine and azithromycin as combined therapy for COVID-19: a case series","authors":"S. Siswanto, O. S. Utama, Agit S. Adisetiadi, Maria E. Pranasakti, M. Hakim","doi":"10.19106/jmedscisi005203202016","DOIUrl":"https://doi.org/10.19106/jmedscisi005203202016","url":null,"abstract":"Coronavirus disease 2019 (COVID-19) is a worldwide outbreak caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The World Health Organization (WHO) has declared it as a public health emergency of international concern (PHEIC) and eventually a pandemic. Many clinical trials have been conducted to investigate potential and effective therapies for COVID-19. Here we reported the outcome of three COVID-19 cases treated early with the combination of hydroxychloroquine and azithromycin. Early treatments of suspected or confirmed positive COVID-19 cases with this combination therapy is to avoid disease progressions into a more severe and irreversible state. In these cases, clinical, radiological, and laboratory features were followed up. No complications were observed. The COVID-19 patients treated with this early combination therapy showed good clinical and virological responses.","PeriodicalId":17474,"journal":{"name":"Journal of thee Medical Sciences (Berkala Ilmu Kedokteran)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87746847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-06-02DOI: 10.19106/jmedsci005203202002
M. Mauny, R. Danarto, D. S. Heriyanto
Study Background : Testicular torsion is a genitourinary emergency most common in children and emergencies requiring second surgery in adolescents after acute appendicitis1. Testicular torsion is more common on the left with a 1.2: 1 ratio, which is probably caused by a slightly longer spermatic cord on the left2. Testicular torsion can occur at any age, but the peak incidence is at age 14, with a peak of second occurrence at 1 year of age3. At 1 year of age, testicular torsion is a major cause of acute scrotum (83%). At the age of 3 - 13 years, the most frequent diagnosis is the torsion of the testicular appendix. After the age of 17 years, epididymitis is the most frequent diagnosis (75%)4. Method: This study is an experimental study, post-test only control group design, the sample was randomly divided into 4 groups, 2 intervention groups and 2 control groups. In this study the independent variables are the duration of the right torsion-detection treatment of the testes and the injection of Methylprednisolone in Wistar male rats. The dependent variables are mRNA expression in apoptosis and anti-apoptosis genes in rats’ ipsilateral and contralateral testes. . Results and Discussion: In this study, two-way ANOVA is used to analyze data between groups. Significant decrease in BAX gene mRNA expression (p <0.05) with Methylprednisolone was present when group 2 (TD) was compared with group 4 (TD-MP). Significant increase in the expression of BCL-2 anti-apoptotic gene (p <0.05) with Methylprednisolone was present when group 1 (T) was compared with group 3 (T-MP). When group 2 (TD) compared with group 4 (TD-MP) also showed a significant increase in the expression of BCL-2 anti-apoptotic gene (p <0.05). Conclusion: The administration of Methylprednisolone in the case of testicular torsion proved to be significant in increasing the expression of the BCL-2 gene that could be a protective mechanism against germ cell apoptosis in testicular tissue.
{"title":"Role of methylprednisolone on mRNA expression of BAX, BCL-2 gene in testicular torsion-detorsion of male albino Wistar rats","authors":"M. Mauny, R. Danarto, D. S. Heriyanto","doi":"10.19106/jmedsci005203202002","DOIUrl":"https://doi.org/10.19106/jmedsci005203202002","url":null,"abstract":"Study Background : Testicular torsion is a genitourinary emergency most common in children and emergencies requiring second surgery in adolescents after acute appendicitis1. Testicular torsion is more common on the left with a 1.2: 1 ratio, which is probably caused by a slightly longer spermatic cord on the left2. Testicular torsion can occur at any age, but the peak incidence is at age 14, with a peak of second occurrence at 1 year of age3. At 1 year of age, testicular torsion is a major cause of acute scrotum (83%). At the age of 3 - 13 years, the most frequent diagnosis is the torsion of the testicular appendix. After the age of 17 years, epididymitis is the most frequent diagnosis (75%)4. \u0000 \u0000Method: This study is an experimental study, post-test only control group design, the sample was randomly divided into 4 groups, 2 intervention groups and 2 control groups. In this study the independent variables are the duration of the right torsion-detection treatment of the testes and the injection of Methylprednisolone in Wistar male rats. The dependent variables are mRNA expression in apoptosis and anti-apoptosis genes in rats’ ipsilateral and contralateral testes. \u0000. \u0000Results and Discussion: In this study, two-way ANOVA is used to analyze data between groups. Significant decrease in BAX gene mRNA expression (p <0.05) with Methylprednisolone was present when group 2 (TD) was compared with group 4 (TD-MP). Significant increase in the expression of BCL-2 anti-apoptotic gene (p <0.05) with Methylprednisolone was present when group 1 (T) was compared with group 3 (T-MP). When group 2 (TD) compared with group 4 (TD-MP) also showed a significant increase in the expression of BCL-2 anti-apoptotic gene (p <0.05). \u0000 \u0000Conclusion: The administration of Methylprednisolone in the case of testicular torsion proved to be significant in increasing the expression of the BCL-2 gene that could be a protective mechanism against germ cell apoptosis in testicular tissue.","PeriodicalId":17474,"journal":{"name":"Journal of thee Medical Sciences (Berkala Ilmu Kedokteran)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86013904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-06-02DOI: 10.19106/jmedsci005203202003
Rahadian Yudo Hartantyo, Mohammad Rizky Mochtar Hidayat, A. Azzam, M. Mulyati
Sporadic dementia of Alzheimer’s type (SDAT) pathogenesis has not been revealed completely due to the difficulty in creating an appropriate animal model. The purpose of this study was to investigate the effect of single-dose intraperitoneal (IP) induction of streptozotocin (STZ) and lipopolysaccharide (LPS) on the β-amyloid levels and the brain function of experimental rats. Eighteen rats were divided into three groups i.e. control, TRE1 (STZ 60 mg.kg-1 BW + LPS 3 mg.kg-1 BW), and TRE2 (STZ 30 mg.kg-1 BW + LPS 1.5 mg.kg-1 BW). The substances were administered in a single dose. Behavioral tests were started at day-30 after injection, we performed Morris water maze (MWM) and novel object recognition (NOR) tests. Afterward, we measured whole brain and serum β-amyloid levels, as one of the biomarkers of Alzheimer’s Disease (AD), using the ELISA method. In MWM tests, the escape latency and time spent in the target quadrant of treatment groups were significantly higher than those in control at the day-5 MWM test and probe trial. The rats in treatment groups have negative discrimination indexes in NOR tasks, indicating that the rats could not remember the familiar object. Intraperitoneal STZ and LPS significantly increase soluble brain β-amyloid levels of treatment groups than those in the control group. In conclusion, the treatment of STZ (60 mg.kg-1 BW) and LPS (3 mg.kg-1 BW) indicated spatial and recognition memory impairment, along with an increase of brain soluble β-amyloid level in rats.
{"title":"Animal model for sporadic dementia of Alzheimer’s type (SDAT) using streptozotocin and lipopolysaccharide combinations in rats","authors":"Rahadian Yudo Hartantyo, Mohammad Rizky Mochtar Hidayat, A. Azzam, M. Mulyati","doi":"10.19106/jmedsci005203202003","DOIUrl":"https://doi.org/10.19106/jmedsci005203202003","url":null,"abstract":"Sporadic dementia of Alzheimer’s type (SDAT) pathogenesis has not been revealed completely due to the difficulty in creating an appropriate animal model. The purpose of this study was to investigate the effect of single-dose intraperitoneal (IP) induction of streptozotocin (STZ) and lipopolysaccharide (LPS) on the β-amyloid levels and the brain function of experimental rats. Eighteen rats were divided into three groups i.e. control, TRE1 (STZ 60 mg.kg-1 BW + LPS 3 mg.kg-1 BW), and TRE2 (STZ 30 mg.kg-1 BW + LPS 1.5 mg.kg-1 BW). The substances were administered in a single dose. Behavioral tests were started at day-30 after injection, we performed Morris water maze (MWM) and novel object recognition (NOR) tests. Afterward, we measured whole brain and serum β-amyloid levels, as one of the biomarkers of Alzheimer’s Disease (AD), using the ELISA method. In MWM tests, the escape latency and time spent in the target quadrant of treatment groups were significantly higher than those in control at the day-5 MWM test and probe trial. The rats in treatment groups have negative discrimination indexes in NOR tasks, indicating that the rats could not remember the familiar object. Intraperitoneal STZ and LPS significantly increase soluble brain β-amyloid levels of treatment groups than those in the control group. In conclusion, the treatment of STZ (60 mg.kg-1 BW) and LPS (3 mg.kg-1 BW) indicated spatial and recognition memory impairment, along with an increase of brain soluble β-amyloid level in rats.","PeriodicalId":17474,"journal":{"name":"Journal of thee Medical Sciences (Berkala Ilmu Kedokteran)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85542857","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-06-02DOI: 10.19106/jmedsci005203202007
A. Wibowo, Herfis Avidati, Sulis Ernawati
Hearing loss is a common occurrence that can decrease productivity and quality of life. The incidence of hearing loss is often experienced by adults due to several factors, and one influencing factor is cardiovascular disease. Hypertension is known to have an effect on decreasing inner ear vascularization that will result in hearing loss. The objective of this study was to evaluate the association of hearing loss and hypertension in non-ear infection patients. This case-control study recruited subjects who underwent audiometric examination at the Universitas Gadjah Mada Academic Hospital, Yogyakarta, from June 2018 - July 2019. Hypertension was determined based on the results of blood pressure examination based on JNC 8 criteria. Hearing threshold value was taken from pure tone audiometry. The association of hypertension with hearing loss was analysed in each group and between study groups. Sixty patients who underwent audiometric examination divided into two groups with 30 patients in each group were involved in this study. There was a significant relationship between hypertension and hearing loss (OR=13.1; 95%CI: 3.8-45.0; p= 0.001). Hypertension acts as a variable that significantly affects the incidence of hearing loss. Patients with hypertension have a greater risk of hearing loss compared to patients without hypertension.
{"title":"Association between hearing loss and hypertension in non ear infection patients in Universitas Gadjah Mada Academic Hospital","authors":"A. Wibowo, Herfis Avidati, Sulis Ernawati","doi":"10.19106/jmedsci005203202007","DOIUrl":"https://doi.org/10.19106/jmedsci005203202007","url":null,"abstract":"Hearing loss is a common occurrence that can decrease productivity and quality of life. The incidence of hearing loss is often experienced by adults due to several factors, and one influencing factor is cardiovascular disease. Hypertension is known to have an effect on decreasing inner ear vascularization that will result in hearing loss. The objective of this study was to evaluate the association of hearing loss and hypertension in non-ear infection patients. This case-control study recruited subjects who underwent audiometric examination at the Universitas Gadjah Mada Academic Hospital, Yogyakarta, from June 2018 - July 2019. Hypertension was determined based on the results of blood pressure examination based on JNC 8 criteria. Hearing threshold value was taken from pure tone audiometry. The association of hypertension with hearing loss was analysed in each group and between study groups. Sixty patients who underwent audiometric examination divided into two groups with 30 patients in each group were involved in this study. There was a significant relationship between hypertension and hearing loss (OR=13.1; 95%CI: 3.8-45.0; p= 0.001). Hypertension acts as a variable that significantly affects the incidence of hearing loss. Patients with hypertension have a greater risk of hearing loss compared to patients without hypertension.","PeriodicalId":17474,"journal":{"name":"Journal of thee Medical Sciences (Berkala Ilmu Kedokteran)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80086471","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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